Single Cell Western Blot Platform

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paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:h1_2_2_text_to_media_multi_cta:field_indv_h1"> <div><h1>Milo Single Cell Western Blot System</h1></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media_multi_cta:field_lg_sngl_frmtd_text_global"> <div><h4><strong>Meet Milo, the World's First Single-Cell Western Platform</strong></h4></div> </div> <div class="field_block:paragraph:h1_2_2_text_to_media_multi_cta:field_call_to_action_global"> <a href="/p/instruments/single-cell-western/request-quote" class="btn btn-primary btn-cta mr-3 mb-3" type="link"> Request Pricing </a> </div> <div class="field_block:paragraph:h1_2_2_text_to_media_multi_cta:field_multi_cta_links"> <div class="mt-3"> <a href="#benefits" class="text-white" type="link"> <u 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id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/instruments">Instruments</a> </li> <li> Single-Cell Western </li> </ol> </nav> </div> </div> </div> </div> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Single Cell Protein Analysis with Milo</h2> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/p/single-cell-western/milo_p100" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/bt-milo-single-cell-western-hero-img.png" alt="Milo Single Cell Western Blot Platform Hero Image" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/p/single-cell-western/milo_p100" > <h3 class="card_title_htag"> <div><h3>Meet Milo</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Milo™ performs Single Cell Western blot analysis on 1,000 single cells in one 4-hour experiment.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/p/single-cell-western/milo_p100" > Learn More About Milo </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/search?keywords=single%20cell%20western&view=condensed&category_attr=Single-Cell%20Western" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/proteinsimple-consumable-SCWestKit.png" alt="Single cell western blot consumables" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/search?keywords=single%20cell%20western&view=condensed&category_attr=Single-Cell%20Western" > <h3 class="card_title_htag"> <div><h3>Milo Consumables</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>The one-stop-shop for all the consumables you need to perform Single Cell Western blot analysis on Milo, including scWest kits, chips, and buffers.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/search?keywords=single%20cell%20western&view=condensed&category_attr=Single-Cell%20Western" > Milo Consumables </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/resources/single-cell-western-antibody-database" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/antibody-floating-480x480.png" alt="Floating Antibody Cell" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/single-cell-western-antibody-database" > <h3 class="card_title_htag"> <div><h3>Milo Antibodies</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Milo is an open platform so you can use any antibody to probe your target. Find antibodies validated for Milo.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/single-cell-western-antibody-database" > Milo Antibody Database </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>The Single Cell Western Blot Has Arrived</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Milo is the world’s first Single Cell Western blotting platform that measures protein expression in ~1000 single cells per run using <a href="/resources/single-cell-western-antibody-database">conventional Western blot antibodies</a>. Simply load your cell suspension on a scWest chip, then Milo performs lysis, SDS-PAGE separation, and UV-induced immobilization of each single cell lysate on-chip in a matter of minutes. Probe the scWest chip with <a href="/resources/single-cell-western-antibody-database">conventional Western blot antibodies</a> and use a microarray scanner to measure ~12 proteins per cell using a variety of <a href="/instruments/single-cell-western/multiplex-single-cell-westerns">multiplexing</a> strategies, or store your chip for up to 9 months for future analysis. </p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="/p/instruments/single-cell-western/request-quote" class="btn btn-primary" type="link"> Request Single Cell Western Blot Pricing </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/research-area-bioprocessing-cell-heterogeneity-750x400.jpg" alt="Bioprocessing Cell Heterogeneity Protein Expression with Milo" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h2><strong>Discover the World of Single Cell Proteomics with Milo</strong></h2> <p>Here are just a few examples of the single cell analysis studies that are made possible with Single Cell Western blot analysis on <a href="/p/single-cell-western/milo_p100">Milo</a>.</p></div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="d-flex flex-column h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/single-cell-western-analysis-hif-1a.png" alt="HIF1-a analysis with single cell western - Milo" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Profile Heterogeneous Proteins and Cell Types</h3></div> </h3> <div class="card_description flex-grow-1"> <div><p>Milo’s single cell heterogeneity analysis profiles heterogeneous proteins and cell types in your samples that would go undetected by <a href="/applications/western-blotting">traditional Western blot</a>.</p> <p><strong><a href="/resources/literature/multiplexed-single-cell-western-analysis-red-blood-cells-for-biomarker-detection">Multiplexed Single Cell Heterogeneity Analysis of Single Red Blood Cells</a></strong></p> <p><strong><a href="/resources/literature/wes-milo-synergize-profile-immune-cell-populations-tumor-microenvironment">Single Cell Heterogeneity Analysis of the Tumor Microenvironment </a></strong></p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="d-flex flex-column h-100"> <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cellxvivo-immune-cell-differentiation-teaser-solo.png" alt="6-well culture dish with different immune cell types in each well" loading="lazy" > </div> </div> </div> <h3 class="card_title_htag"> <div><h3>Monitor Stem Cell Proteomics</h3></div> </h3> <div class="card_description flex-grow-1"> <div><p>Check out these App Notes to learn how Single Cell Western blotting with Milo can track and quantify single <a href="/research-areas/stem-cells">stem cell</a> proteomics and differentiation into <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-neuroscience">neural cell subtypes</a> and cardiomyocytes:</p> <p><strong><a href="/resources/literature/identify-quantify-neural-subtypes-single-cell-westerns">Identify and Quantify Neural Cell Subtypes</a></strong></p> <p><strong><a href="/resources/literature/mending-myocardium-tracking-cardiomyocyte-differentiation-single-cell-westerns">Track iPSC to Cardiomyocyte Differentiation</a></strong></p></div> </div> <div class="card-bottom-link"></div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="benefits"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_header_2_global"> <div><h2>Easier Than Single Cell Flow Cytometry</h2></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Milo is easier to use than other single cell analysis technologies like single cell flow cytometry. With Milo’s single cell analysis, you can detect proteins in single cells that escape detection by single cell <a href="/resources/instrument-applications/flow-cytometry-and-facs-cell-sorting-milo">flow cytometry</a> due to their intracellular location or due to the lack of flow cytometry-validated antibodies. You can also pair Milo with fluorescence-activated cell sorting (FACS) to sort cell subtypes or live/dead cells for downstream single cell analysis on Milo.</p> <p><strong><a href="/resources/literature/going-with-flow-milo-streamline-flow-cytometry-experiments">Learn How Milo Streamlines Your Single Cell Flow Cytometry Experiments</a></strong></p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/why-single-cell-westerns-graphs.jpg" alt="Milo’s single cell protein analysis" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/resources/literature/single-cell-westerns-advance-sickle-cell-disease-gene-therapies" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/single-cell-western-cell-gene-therapy-sickle-cell.png" alt="Single-cell Western Cell and Gene Therapy - sickle cell analysis " loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/single-cell-westerns-advance-sickle-cell-disease-gene-therapies" > <h3 class="card_title_htag"> <div><h3>Develop New Cell and Gene Therapies</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Reveal the functional potency of viral vectors and other gene delivery systems. Measure their gene editing efficiency by monitoring target protein expression in single cells following genetic transformation to develop new <a href="/resources/instrument-applications/accelerate-your-cell-gene-therapy-workflows-with-advanced-western-blotting-solutions">cell and gene therapies</a>.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/single-cell-westerns-advance-sickle-cell-disease-gene-therapies" > Track Gene Editing Efficiency </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/instruments/single-cell-western/protein-validation-of-your-single-cell-rna-sequencing-data-milo" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/protein-validation-workflow-milo.png" alt="Milo protein validation workflow of single-cell RNA seq data" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/instruments/single-cell-western/protein-validation-of-your-single-cell-rna-sequencing-data-milo" > <h3 class="card_title_htag"> <div><h3>Validate Single Cell Sequencing Data</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Pair Milo’s single cell proteomics with single cell RNA sequencing workflows for complete single cell multiomic analysis. Milo provides single cell protein expression information to <a href="/instruments/single-cell-western/protein-validation-of-your-single-cell-rna-sequencing-data-milo">validate your single cell RNA seq analysis single cell protein expression data</a>. </p></div> </div> <div class="card-bottom-link"> <div> <a href="/instruments/single-cell-western/protein-validation-of-your-single-cell-rna-sequencing-data-milo" > Validate scRNA Seq Analysis </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_header_2_global"> <div><h2>Publish Your Single Cell Western Blots in Premier Journals</h2></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p>Milo has proven its ability to provide new and profound scientific insight that <a href="/applications/western-blotting">traditional Western blots</a> cannot. This fact is reflected in the rank of the journals that feature articles with Single Cell Western blot data with the vast majority (74%) of articles featuring Single Cell Western blot data in top journals with impact factors of 7 or greater including <em>Science</em>, <em>Cell</em>, <em>Nature Methods</em>, <em>Nature Communications, </em>and <em>Cell Reports</em>. 32% of <a href="/resources/instrument-citations?instrument-platform=single-cell%20western">Single Cell Western blot publications</a> are in journals considered here to be in the premier category with an impact factor greater than 21.</p> <p><strong><a href="/resources/literature/simple-western-produces-publication-ready-results">Learn How Single Cell Western Blot is a Proven, High-Impact Technology</a></strong></p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/publication-standards-tpn-2-525x200.png" alt="Protein Simple let's you meet scientific standards by delivering publication-ready results" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="resources"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--_-1-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--75-25"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_header_2_global"> <div><h2>Additional Single Cell Western Blot Resources</h2></div> </div> <div class="field_block:paragraph:3_1_text_to_media:field_lg_sngl_frmtd_text_global"> <div><ul> <li><a href="/resources/instrument-citations?instrument-platform=single-cell%20western">Search the Single Cell Western Blot Publication Database</a></li> <li><a href="/resources/literature/improving-antibody-performance-signal-enhancement-reagent-milo">Improve Antibody Performance with the Signal Enhancement Reagent</a></li> <li><a href="/resources/literature/dissociation-of-mouse-neural-tissue-single-cell-western-analysis">Learn How to Dissociate Neural Tissue for Analysis on Milo</a></li> <li><a href="/instruments/single-cell-western/single-cell-western-faqs">Single Cell Western FAQs</a></li> </ul></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:3_1_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/single-cell-genomics-icon.png" alt="Bio-Techne instruments can be applied to study single cell genomics" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="thin-banner banner_wrapper"> <div class="banner-dark"> <div> <a href="https://resources.bio-techne.com/bio-techne-assets/docs/literature/Milo_ProductBrochure_RevD_DIGITAL.pdf" > <button type="button" class="btn btn-primary"> Download the Milo Brochure </button> </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="testimonial"> <div > <div class="row"> <div class="col-md-4"> <div class="left-title"> <h2>Customer Success Stories</h2> </div> </div> <div class="col-md-8"> <div class="right-card bg-white border rounded p-5 mb-4"> <div class="carousel slide" id="carouselIndicators" data-ride="carousel"> <div class="row"> <div class="col-md-12"> <div class="carousel-inner"> <div class="carousel-item active">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-b788f911dd3" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/testimonials/milo-single-cell-western-chia-wei-cheng.png' alt='Chia-Wei Cheng, Ph.D.'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Chia-Wei Cheng, Ph.D. </span><br/> <span class="individual-testimonial__position"> Postdoctoral Fellow, MIT </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> With Milo, we observed a level of single cell protein expression heterogeneity in our purified intestinal stem cell population that was not previously possible with conventional Western blotting. </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10 mt-2"> <a class="font-weight-bold" href="/resources/testimonials/cheng-milo-goes-keto">Read More<span class="icon-cheveron-right"></span></a> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-bbb6ce93167" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/testimonials/single-cell-western-maya-kaelberer.png' alt='M. Maya Kaelberer Thumbnail'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> M. Maya Kaelberer, Ph.D. </span><br/> <span class="individual-testimonial__position"> Postdoctoral Associate, Duke University School of Medicine </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> We have been pairing Milo with single cell real time qPCR data. We have the RNA and we know the transcripts are present. We used Milo to confirm our results and quantify the amount of protein that is in each individual cell. </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10 mt-2"> <a class="font-weight-bold" href="/resources/testimonials/milo-validates-neuroepithelial-circuit">Read More<span class="icon-cheveron-right"></span></a> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-92cf659c099" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/testimonials/single-cell-western-yi-zhong.png' alt='Yi Zhong Thumbnail'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Yi Zhong, M.D., Ph.D. </span><br/> <span class="individual-testimonial__position"> Research Associate, Memorial Sloan Kettering Cancer Center </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> Milo permits study of proteins at the single cell level to understand the heterogeneity among metastatic cells within a population. </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10 mt-2"> <a class="font-weight-bold" href="/resources/testimonials/milo-heterogeneity-in-metastatic-cell-populations">Read More<span class="icon-cheveron-right"></span></a> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-342a9017d22" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/testimonials/single-cell-western-prashant.png' alt='Prashant Vijay Thakkar Thumbnail'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Prashant Vijay Thakkar, Ph.D. </span><br/> <span class="individual-testimonial__position"> Postdoctoral associate, Weill Cornell Medicine </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> I was looking at two different isoforms of a protein, and it was important to know if the cells were expressing just one versus the other or both in a given cell. That was tricky until Milo came. We’re also getting more relevant information because Milo allows us to look at expression of this protein in tissue biopsies. </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10 mt-2"> <a class="font-weight-bold" href="/resources/testimonials/milo-taxane-resistance-at-the-single-cell-level">Read More<span class="icon-cheveron-right"></span></a> </div> </div> </div> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <ol class="carousel-indicators"> <li data-target="#carouselIndicators" data-slide-to="0" class="active"> </li> <li data-target="#carouselIndicators" data-slide-to="1"> </li> <li data-target="#carouselIndicators" data-slide-to="2"> </li> <li data-target="#carouselIndicators" data-slide-to="3"> </li> </ol> </div> </div> <a class="carousel-control-prev" href="#carouselIndicators" role="button" data-slide="prev"> <span class="carousel-control-prev-icon" aria-hidden="true"></span> <span class="sr-only">Previous</span> </a> <a class="carousel-control-next" href="#carouselIndicators" role="button" data-slide="next"> <span class="carousel-control-next-icon" aria-hidden="true"></span> <span class="sr-only">Next</span> </a> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><h3>Requirements and Compatibility</h3> <figure class="responsive-figure-table"><table class="overflow-auto table table-bordered"> <thead> <tr> <th> <p>Sample type</p> </th> <th> <p>Suspension containing >10,000 cells</p> </th> </tr> </thead> <tbody> <tr> <td> <p>Cell diameter</p> </td> <td> <p>7-25 µm in suspension</p> </td> </tr> <tr> <td> <p>Cell type</p> </td> <td> <p>Mammalian cells; globular in suspension and unfixed</p> </td> </tr> <tr> <td> <p>Antibody requirement</p> </td> <td> <p>Standard unlabeled primaries and fluorescent secondaries</p> </td> </tr> <tr> <td> <p>Other equipment needed</p> </td> <td> <p>Open-format fluorescence microarray scanner capable of 5 µm resolution</p> </td> </tr> </tbody> </table></figure> <p> </p> <h3>Performance and Specifications</h3> <figure class="responsive-figure-table"><table class="overflow-auto table table-bordered"> <thead> <tr> <th> <p>Typical cell dilutions</p> </th> <th> <p>Yield capture and analysis of 1,000-2,000 cells per scWest chip</p> </th> </tr> </thead> <tbody> <tr> <td> <p>Molecular weight (MW) range</p> </td> <td> <p>15-175 kDa</p> </td> </tr> <tr> <td> <p>MW resolution</p> </td> <td> <p>10% difference in distinct spectral channels, as low as 30% differences in same spectral channel</p> </td> </tr> <tr> <td> <p>Typical target multiplexing</p> </td> <td> <p>Up to four proteins per cell by spectral and size-based multiplexing<br> Twelve-plus proteins per cell using stripping & reprobing</p> </td> </tr> <tr> <td> <p>Workflow time</p> </td> <td> <p>4-6 hours</p> </td> </tr> </tbody> </table></figure></div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <h2>Single Cell Western Blot Applications</h2> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-oncology" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/cancer-immune-cells-525x315-2.jpg" alt="immune cells attack cancer cell" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/advanced-western-blotting-solutions-for-oncology" > <h3 class="card_title_htag"> <div><h3>Cancer and Immuno-Oncology</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Transform your cancer & immuno-oncology research by utilizing next-generation Western blotting tools to give you richer views into your samples.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-oncology" > Cancer and Immuno-Oncology </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/accelerate-your-cell-gene-therapy-workflows-with-advanced-western-blotting-solutions" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/webinars/webinar-aav-lentivirus-thumbnail-350x200.png" alt="Testing for Lentivirus and AAVs" loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/accelerate-your-cell-gene-therapy-workflows-with-advanced-western-blotting-solutions" > <h3 class="card_title_htag"> <div>Cell and Gene Therapy</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/accelerate-your-cell-gene-therapy-workflows-with-advanced-western-blotting-solutions" > Tools for Cell and Gene Therapy </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/instruments/simple-western/applications/advanced-western-blotting-solutions-for-cell-signaling" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/cell-signaling-teaser-v2-525x315.jpg" alt="Solutions for Cell Signaling and Signal Pathways Analysis" loading="lazy" > </div> </a> <a class="card_title_link" href="/instruments/simple-western/applications/advanced-western-blotting-solutions-for-cell-signaling" > <h3 class="card_title_htag"> <div>Cell Signaling</div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Uncover phospho-protein heterogeneity and quantify how phosphorylation varies across stimulated and unstimulated cell populations.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/instruments/simple-western/applications/advanced-western-blotting-solutions-for-cell-signaling" > Cell Signaling Pathways </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="bg-white border rounded flex-column d-flex h-100"> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-neuroscience" > <div class="teaser-card-image-crop mb-3"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/ella-apps-neuroscience-2.jpg" alt="Neuroscience App image for Ella " loading="lazy" > </div> </a> <a class="card_title_link" href="/resources/instrument-applications/advanced-western-blotting-solutions-for-neuroscience" > <h3 class="card_title_htag"> <div><h3>Neuroscience</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems.</p></div> </div> <div class="card-bottom-link"> <div> <a href="/resources/instrument-applications/advanced-western-blotting-solutions-for-neuroscience" > Solutions for Neuroscience </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </article> </div> </div> </section> </main> </div> <div id="brandsbar-footer" class="bg-bt-primary"> <div class="container-wide container-fluid text-white"> <div class="row py-5"> 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