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class="breadcrumb-link-text-wrap">Clinical Diagnostics</span></a><meta ></span> <span aria-label="breadcrumb separator">/</span> <span class="breadcrumb-current-title">Mpox surge and public health laboratories</span></div></div></header><div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-968 post type-post status-publish format-standard category-clinical-diagnostics tag-monkeypox entry" aria-label="Mpox surge and public health laboratories"><header class="entry-header"><h1 class="entry-title">Mpox surge and public health laboratories</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/askascientiststaff/" title="Posts by Ask A Scientist Staff" rel="author">Ask A Scientist Staff</a> </span> <span class="post-info-date"> 09.23.2024 </span> </header><div class="entry-content"><h2>Mpox cases</h2> <div style="background-color: #e2e3e4;padding: 20px;width: 30%;float: right;margin: 0 0 0 20px"> <h5>Learn more about our infectious disease testing solutions to support your research <a href="https://www.thermofisher.com/us/en/home/global/forms/life-science/infectious-disease-testing-solutions.html">here.</a></h5> </div> <p><span data-contrast="auto">With public health officials reporting more than 15,600 cases of mpox infections and 537 deaths in Central and East African countries, the WHO Director-General has “determined that the upsurge of mpox in the Democratic Republic of the Congo (DRC) and a growing number of countries in Africa constitutes a public health emergency of international concern.”</span><span data-contrast="auto"><sup>1</sup></span><span data-ccp-props="{"201341983":0,"335559739":160,"335559740":259}"> </span></p> <p><span data-contrast="auto">The clade 1 mpox variant, responsible for the majority of current infections, has been detected in neighboring DRC countries, and in an increasing number of non-African countries where this variant is not known to be endemic.</span><span data-contrast="auto"><sup>2,3</sup></span><span data-ccp-props="{"201341983":0,"335559739":160,"335559740":259}"> To date, only one case outside the endemic region has been confirmed, suggesting limited transmission to-date outside of Africa. </span></p> <h2>Public health labs respond to mpox virus</h2> <p><span data-contrast="auto">The CDC has issued an updated Health Alert Network advisory recommending that clinicians and laboratories “s</span><span data-contrast="none">end clinical specimens collected from patients who traveled from DRC or its neighboring countries, or had close or intimate contact with symptomatic people from these countries, to a laboratory that can perform clade-specific testing </span><span data-contrast="none">as quickly as possible”.</span><span data-contrast="none"><sup>2</sup></span><span data-ccp-props="{"201341983":0,"335559739":160,"335559740":259}"> </span></p> <p><span data-contrast="auto">For the most up-to-date information, please see the </span><a href="https://www.cdc.gov/poxvirus/mpox/outbreak/index.html"><span data-contrast="none">CDC Mpox outbreak</span></a><span data-contrast="auto"> website.</span><span data-ccp-props="{"201341983":0,"335559739":160,"335559740":259}"> </span></p> <p><span data-contrast="auto">WHO prefers detection of viral DNA by PCR as antigen- and antibody-based methods typically do not distinguish between Orthopoxviruses. Specimens collected directly from the rash—skin, fluid or crusts, or biopsy where feasible—are preferred.</span><span data-contrast="auto"><sup>4</sup></span><span data-ccp-props="{"201341983":0,"335559739":160,"335559740":259}"> </span></p> <div></div> <div style="background-color: #9bd3dd;padding: 20px;width: 100%"> <h2>What is Mpox virus (MPX or MPXV)?</h2> <ul> <li>Mpox is a DNA virus endemic to Central and West African countries.</li> <li>An Orthopoxvirus, mpox is part of the genus of viruses that cause diseases like smallpox. It is associated with skin lesions and lymph node swelling among other symptoms.</li> <li>Tropical rainforest animals including rodents and non-human primates are the primary carriers of mpox.</li> <li>There are two distinct clades of MPXV, a West African clade and a Congo Basin clade. People infected by virus from the Congo Basin/Central African clade experience approximately 10% case fatality rate while those infected by the West African clade experience a <4% case fatality rate.<sup>3</sup></li> </ul> </div> <h2><a name="orderinginfo"></a></h2> <div> <p class="p1">[1] If RNA and DNA analyses are performed on the same plate using a common thermal cycling protocol, the TaqPath™ 1‑Step Multiplex Master Mix (No ROX™) (Cat. No. <span class="s1">A28522</span>) can be used instead of TaqPath™ BactoPure™ Microbial Detection Master Mix (No ROX™).</p> <p><em>For research use only. Not for use in diagnostic procedures.</em></p> <p><strong>Sources</strong></p> <ol> <li><a href="https://www.who.int/news/item/14-08-2024-who-director-general-declares-mpox-outbreak-a-public-health-emergency-of-international-concern"><span data-contrast="none"><span data-ccp-charstyle="Hyperlink">https://www.who.int/news/item/14-08-2024-who-director-general-declares-mpox-outbreak-a-public-health-emergency-of-international-concern</span></span></a></li> <li><a href="https://emergency.cdc.gov/han/2024/han00513.asp"><span data-contrast="none"><span data-ccp-charstyle="Hyperlink">https://emergency.cdc.gov/han/2024/han00513.asp</span></span></a></li> <li><a href="https://www.folkhalsomyndigheten.se/the-public-health-agency-of-sweden/communicable-disease-control/disease-information-about-mpox/one-case-of-mpox-clade-i-reported-in-sweden/"><span data-contrast="none"><span data-ccp-charstyle="Hyperlink">https://www.folkhalsomyndigheten.se/the-public-health-agency-of-sweden/communicable-disease-control/disease-information-about-mpox/one-case-of-mpox-clade-i-reported-in-sweden/</span></span></a></li> <li><a href="https://www.who.int/publications/i/item/WHO-MPX-Laboratory-2024.1"><span data-contrast="none"><span data-ccp-charstyle="Hyperlink">https://www.who.int/publications/i/item/WHO-MPX-Laboratory-2024.1</span></span></a></li> </ol> <p>© 2022 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. 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