Influence of Organic Supplements on Shoot Multiplication Efficiency of Phaius tankervilleae var. alba

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publication-listing mt-3 mb-3"> <h5 class="card-header" style="font-size:.9rem">Influence of Organic Supplements on Shoot Multiplication Efficiency of Phaius tankervilleae var. alba</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=T.%20Punjansing">T. Punjansing</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Nakkuntod"> M. Nakkuntod</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Homchan"> S. Homchan</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Inthima"> P. Inthima</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Kongbangkerd"> A. Kongbangkerd</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The influence of organic supplements on growth and multiplication efficiency of <em>Phaius tankervilleae </em>var. <em>alba</em> seedlings was investigated. 12 week-old seedlings were cultured on half-strength semi-solid Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose, 8 g/L agar and various concentrations of coconut water (0, 50, 100, 150 and 200 mL/L) combined with potato extract (0, 25 and 50 g/L) and the pH was adjusted to 5.8 prior to autoclaving. The cultures were then kept under constant photoperiod (16 h light: 8 h dark) at 25 ± 2 °C for 12 weeks. The highest number of shoots (3.0 shoots/explant) was obtained when cultured on the medium added with 50 ml/L coconut water and 50 g/L potato extract whereas the highest number of leaves (5.9 leaves/explant) and roots (6.1 roots/explant) could receive on the medium supplemented with 150 ml/L coconut water and 50 g/L potato extract. with 150 ml/L coconut water and 50 g/L potato extract. Additionally, plantlets of<em> P</em>. <em>tankervilleae </em>var. <em>alba</em> were transferred to grow into seven different substrates i.e. soil, sand, coconut husk chip, soil-sand mix (1: 1), soil-coconut husk chip mix (1: 1), sand-coconut husk chip mix (1: 1) and soil-sand-coconut husk chip mix (1: 1: 1) for four weeks. The results found that acclimatized plants showed 100% of survivals when sand, coconut husk chip and sand-coconut husk chip mix are used as substrates. The number of leaves induced by sand-coconut husk chip mix was significantly higher than that planted in other substrates (<em>P</em> > 0.05). Meanwhile, no significant difference in new shoot formation among these substrates was observed (<em>P</em> < 0.05). This precursory developing protocol was likely to be applied for more large scale of plant production as well as conservation of germplasm of this orchid species. <iframe src="https://publications.waset.org/abstracts/95318.pdf" style="width:100%; height:400px;" frameborder="0"></iframe> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=organic%20supplements" title="organic supplements">organic supplements</a>, <a href="https://publications.waset.org/abstracts/search?q=acclimatization" title=" acclimatization"> acclimatization</a>, <a href="https://publications.waset.org/abstracts/search?q=Phaius%20tankervilleae%20var.%20alba" title=" Phaius tankervilleae var. alba"> Phaius tankervilleae var. alba</a>, <a href="https://publications.waset.org/abstracts/search?q=orchid" title=" orchid"> orchid</a> </p> <a href="https://publications.waset.org/abstracts/95318/influence-of-organic-supplements-on-shoot-multiplication-efficiency-of-phaius-tankervilleae-var-alba" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/95318.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">231</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> 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Influence of Organic Supplements on Shoot Multiplication Efficiency of Phaius tankervilleae var. alba