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class="breadcrumb-current-title">Improved Method to Identify and Quantify Antibiotics in Chicken Meat</span></div></div></header><div class="site-inner"><div class="content-sidebar-wrap"><main class="content"><article class="post-1780 post type-post status-publish format-standard has-post-thumbnail category-chemical-elemental-analysis tag-antibiotics tag-lc-msms tag-poultry entry" aria-label="Improved Method to Identify and Quantify Antibiotics in Chicken Meat"><header class="entry-header"><h1 class="entry-title">Improved Method to Identify and Quantify Antibiotics in Chicken Meat</h1> <span class="post-info-author"> By <a href="https://www.thermofisher.com/blog/author/emilyhumphreys/" title="Posts by Emily Humphreys" rel="author">Emily Humphreys</a> </span> <span class="post-info-date"> 10.31.2013 </span> </header><div class="entry-content"><p><img decoding="async" class="alignright" style="float: right;" alt="raw chicken" src="https://www.thermofisher.com/blog/wp-content/uploads/2013/10/raw_chicken_200_across.jpg" width="200" height="157" />In Europe, the <a href="http://ec.europa.eu/food/index_en.htm" target="_blank">European Commission</a> maintains specific guidelines to regulate drug residues found in products of animal origin and ensure they are maintained at safe levels. For these guidelines to be effective, sensitive screening methods are needed to detect drugs residues in various samples, including meat products. <a href="http://en.wikipedia.org/wiki/Liquid_chromatography%E2%80%93mass_spectrometry" target="_blank">L</a><span><a href="http://en.wikipedia.org/wiki/Liquid_chromatography%E2%80%93mass_spectrometry" target="_blank">iquid chromatography–tandem mass spectrometry</a> (LC-MS/MS) has the ability to target a wide range of drug classes and has been used previously to screen different antibiotics in muscle and kidney samples.<sup>1</sup></span><sup> </sup>Scientists have now developed an improved strategy to identify and quantify veterinary drugs in meats using LC-MS/MS.<sup>2 </sup>This newly developed method has the ability to identify and quantify up to 36 different antibiotics from seven different chemical classes including <a href="http://en.wikipedia.org/wiki/Aminoglycoside" target="_blank">aminoglycosides</a>, <a href="http://en.wikipedia.org/wiki/Macrolide" target="_blank">macrolides</a>, <a href="http://en.wikipedia.org/wiki/Lincosamides" target="_blank">lincosamides</a>, <a href="http://en.wikipedia.org/wiki/Sulfonamide_%28chemistry%29" target="_blank">sulfonamides</a>, <a href="http://en.wikipedia.org/wiki/Tetracycline" target="_blank">tetracyclines</a>, <a href="http://en.wikipedia.org/wiki/Quinolone" target="_blank">quinolones</a> and <a href="http://en.wikipedia.org/wiki/Trimethoprim" target="_blank">trimethoprim</a>. Key to the success of this protocol was the advanced instrumentation employed by the scientists. Sample cleanup and chromatographic separation was completed using a <span>turbulent ﬂow chromatography <a href="http://www.thermoscientific.com/ecomm/servlet/productsdetail?storeId=11152&productId=11962145&ca=transcend" target="_blank">Transcend TLX-1 system</a> using Turbo Flow columns</span> (all from Thermo Scientific). Its automated platform eliminated the need for manual sample handling, thus being advantageous in both efficiency and cost effectiveness. The turbulent flow chromatography system was coupled to a <a href="http://www.thermoscientific.com/ecomm/servlet/productsdetail_11152___11961854_-1" target="_blank">TSQ Quantum Access Max triple quadruple mass spectrometer</a> (Thermo Scientific). Data was acquired using the selected reaction monitoring mode (SRM), with the SRM transitions finely tuned to each target <a href="http://en.wikipedia.org/wiki/Analyte" target="_blank">analyte</a>. Quantifier and qualifier transition ions confirmed the specificity of this protocol, and the precision was determined using spiked samples in replicate, as compared in different runs on different days. All in all, the accuracy was determined to be within acceptable ranges. To demonstrate the validity of this approach, the scientists examined the 20 samples of chicken meat and meat products purchased from a local market for traces of antibiotics. J<span>ust one out of the 36 antibiotics was present and in only one of the samples analyzed.</span> The drug, <a href="http://en.wikipedia.org/wiki/Enrofloxacin" target="_blank">enroﬂoxacin</a>, was found in chicken ham, and was far below the acceptable limit for veterinary drugs present in meats. After validating this protocol in-house according to the criteria speciﬁed for quantitative methods described in the European Commission Decision 2002/675/EC<sup>3</sup>, the researchers recommended this protocol to be used to enforce the limits described for veterinary drugs in consumable meats. References </p> <ol> <li>Granelli, K. & Branzell, C. (2007) “<a href="http://www.sciencedirect.com/science/article/pii/S0003267006023919">Rapid multi-residue screening of antibiotics in muscle and kidney by liquid chromatography-electrospray ionization-tandem mass spectrometry</a>.”, Analytica Chimica Acta 586(1-2) (pp. 289-952)</li> <li>Bousova, K., Senyuva, H. & Mittendorf, K. (2013) “<a href="http://www.ncbi.nlm.nih.gov/pubmed/23298848">Quantitative multi-residue method for determination antibiotics in chicken meat using turbulent ﬂow chromatography coupled to liquid chromatography–tandem mass spectrometry</a>.”, Journal of Chromatography A, 1274 (pp. 19-27)</li> <li>Commission Decision 2002/657/EC of 12th August implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of the results, Official of the Journal of European Communications L221(2002)8</li> </ol> <p> <em>Have you found this interesting? Like to know more? 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