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font-weight: 300 !important; font-family: "Roboto Slab", helvetica, arial, sans-serif; top: 55px; left: 0px; background: #eab3a0; margin: 10px 0; padding: 10px 15px; } .hs-richtext { margin-bottom: 3px; font-family: "Roboto Slab", helvetica, arial, sans-serif; /* color: #FFFFFF;*/ font-weight: 400; font-size: 16px; line-height: 26px; } /* Hide the original checkbox */ ul.inputs-list.multi-container li.hs-form-checkbox label input, .hs-form-booleancheckbox-display input { height: 24px; left: 0; opacity: 0; position: absolute; top: 0; width: 24px; } /* Add new checkbox */ ul.inputs-list.multi-container li.hs-form-checkbox label span::before, .hs-form-booleancheckbox-display span::before { content: ""; border-radius: 0px; background-color: #ffffff; position: absolute; top: 4px; left: 8px; z-index: 1; display: block; width: 5px; height: 10px; border: solid #157479; border-width: 0 2px 2px 0; -webkit-transform: rotate(45deg); -ms-transform: rotate(45deg); transform: rotate(45deg); opacity: 0; -webkit-transition-duration: .1s; -o-transition-duration: .1s; transition-duration: .1s; -webkit-transition-property: border-color; -o-transition-property: border-color; transition-property: border-color; } /* Style new checked item */ ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hs-form-booleancheckbox-display span::after { content: ""; border: 2px solid #d9d9d9; border-radius: 3px; height: 20px; left: 0px; position: absolute; top: 0px; width: 20px; background: #fff; z-index: 0; display: block; transition-property: background-color, border-color, -webkit-box-shadow; -o-transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow; transition-property: background-color, border-color, box-shadow, -webkit-box-shadow; } /* Show when checked */ ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::after, .hs-form-booleancheckbox-display input:checked + span::after, ul.inputs-list.multi-container li.hs-form-checkbox label input:checked + span::before, .hs-form-booleancheckbox-display input:checked + span::before{ opacity: 1; } .hs-form-booleancheckbox-display input:checked+label:after, .hs-form-booleancheckbox-display input:checked+label:after { background: #fff; border-color: #157479; } /* Style when focused */ ul.inputs-list.multi-container li.hs-form-checkbox label input:focus + span::after, .hs-form-booleancheckbox-display input:focus + span::after { outline: 3px solid transparent; /* For Windows high contrast mode. */ box-shadow: 0 0 .3125rem rgba(0, 0, 0, .3); border-color: #157479; } .hs_contact_country{ clear: both; } .hs-button { border-radius: 65px !important; font-size: 14px; line-height: 1.3; letter-spacing: 1.12px; font-family: "Roboto Slab", helvetica, arial, sans-serif; font-weight: 700 !important; padding: 19px 23px !important; border: 2px solid; display: inline-block; text-decoration: none; text-transform: uppercase; -webkit-appearance: none !important; -moz-appearance: none !important; appearance: none !important; } .hbspt-form textarea{max-width: 100% !important; height: 186px !important; resize: none;} .hbspt-form textarea:focus{border: 1px solid #74cbc8 !important;} .hs-fieldtype-textarea .invalid.error{border: 2px solid #E74949 !important;} .hbspt-form fieldset{max-width: 100% !important; margin-bottom: 60px;} .hbspt-form .field {margin-bottom: 27px} .hs_recaptcha{ margin-top: 23px !important; margin-bottom: 27px !important; } .hs-button, .hs-form-field input[type=text], .hs-form-field input[type=email], .hs-form-field input[type=phone], .hs-form-field input[type=number], .hs-form-field input[type=tel], .hs-form-field input[type=date], .hs-form-field textarea { appearance: none; -webkit-appearance: none; -moz-appearance: none; } .hs-default-font-element, .hs-main-font-element { font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hbspt-form .hs-button { white-space: pre-wrap } .hbspt-form .hs-richtext { word-break: break-word } .hbspt-form.submitted-message { overflow: auto } .hbspt-form fieldset.form-columns-1 .hs-input { width: 95% } .hbspt-form fieldset.form-columns-1 .input { margin-right: 0px !important } .hbspt-form fieldset.form-columns-1 input[type=checkbox], .hbspt-form fieldset.form-columns-1 input[type=radio] { width: auto } .hbspt-form fieldset.form-columns-2 .hs-form-field { width: 50%; float: left } .hbspt-form fieldset.form-columns-2 .input { margin-right: 0px !important; } .hbspt-form fieldset.form-columns-3 .hs-form-field { width: 32.7%; float: left } .hbspt-form fieldset.form-columns-3 .input { margin-right: 8px } .hbspt-form label.hs-hidden { visibility: hidden } .hbspt-form .hs-field-desc { width: 100% } .hs-custom-style .hs-input, .hs-custom-style fieldset { max-width: 100% } .hs-custom-style>div.form-columns-3 .hs-form-field, .hs-custom-style fieldset.form-columns-3 .hs-form-field { width: 33.3% } .hs-custom-style>div>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]), .hs-custom-style fieldset>div:last-of-type .hs-input:not([type=checkbox]):not([type=radio]) { width: 100%; max-width: 100% } .hs-custom-style>div input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]), .hs-custom-style fieldset input:not([type=image]):not([type=submit]):not([type=button]):not([type=radio]):not([type=checkbox]):not([type=file]) { box-sizing: border-box; padding: 0 15px; min-height: 27px } .hs-custom-style>div textarea, .hs-custom-style fieldset textarea { padding: 10px 15px } .hs-custom-style .hs-dependent-field>div .hs-input:not([type=checkbox]):not([type=radio]) { width: 100% } .legal-consent-container .field.hs-form-field { margin-bottom: 8px } .legal-consent-container .hs-field-desc.checkbox-desc { margin: -12px 0 0 21px } .legal-consent-container .hs-form-booleancheckbox-display input { float: left } .legal-consent-container .hs-form-booleancheckbox-display>span { display: block !important; margin-left: 30px !important; } .legal-consent-container .hs-form-booleancheckbox-display p { margin: 0; display: inline } .legal-consent-container .hs-error-msgs label { color: #f2545b } .legal-consent-container~.hs_recaptcha { margin-top: 18px } .cookie-reset-container { font-size: 14px; margin-bottom: 10px; text-align: right } #hs-outer-captcha-target, #hs-outer-captcha-target * { display: none; height: 0; width: 0 } .hubspot-link__container { font-size: 14px; padding-bottom: 40px; position: relative; color: #9fa0a2; font-family: "Roboto Slab", helvetica, arial, sans-serif; } .hubspot-link-text { color: #00a4bd; font-weight: 400 } .hubspot-link__container.sproket { color: #9fa0a2 } .hubspot-link { color: #9fa0a2 } .hubspot-link, .hubspot-link:hover { text-decoration: none } .hubspot-link:hover .hubspot-link-text { text-decoration: underline } .hubspot-link__icon { margin-bottom: -1px; margin-right: 5px } .hubspot-link__container.sproket .hubspot-link__icon { width: 30px; margin-right: 0; float: left; margin-top: -9px; margin-left: -5px } .hubspot_signup_form .hs_email{margin-bottom: 0px !important; width: 80% !important; float: left !important;} .hubspot_signup_form .hs_submit{width: 20% !important; float: left !important; } .hubspot_signup_form .hs-button{padding: 12.5px 15px !important;} .hubspot_signup_form .hs_email label{display: none !important;} .hubspot_signup_form ul.no-list{position: absolute !important; top: 48px !important;} .hubspot_signup_form {position: relative !important;} .hubspot_signup_form .submitted-message{color: #ab8940 !important; font-size: 22px !important; } .hubspot_white .hbspt-form label, .hubspot_white .hs-form-required { color: #fff; } .hubspot_white .hs-input, .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::before, .hubspot_white .hs-form-booleancheckbox-display span::before{ background-color: #fff !important; } .hubspot_white .hs_submit { text-align: center; } .hubspot_white .hs-form select.is-placeholder { opacity: 1; } .hubspot_white ul.inputs-list.multi-container li.hs-form-checkbox label span::after, .hubspot_white .hs-form-booleancheckbox-display span::after { border-color: #3f4852; } input[type=submit].hs-button.primary.large{ color: #fff; background: #00181e; border-color: #00181e !important; } input[type=submit].hs-button.primary.large:hover { background: #74cbc8 !important; border-color: #74cbc8 !important; } /***/ .hbspt-form .hs-input[type=checkbox]{ cursor: pointer; } .hs-form-booleancheckbox-display input:checked + span::after{ background: #fff; border-color: #157479; } .hbspt-form .form-columns-2 { display: flex; flex-flow: row wrap; -webkit-box-direction: normal; } .hbspt-form .hs_firstname { flex: 1; -webkit-box-flex: 1; } .hbspt-form .hs_submit{ margin-top: 60px; } .newsletter-form .hs_submit{margin-top: 45px;} .newsletter-form form .gfield_consent_description { border: none !important; } /*New Landing page*/ .landing-middle-section{ padding-right: 0px !important; } .left-col{max-width:93%} .left-col-para{padding-right:30px;} .left-col video{border-radius:4px;} /* Kit asssesment */ .kits_form_col .hbspt-form .hs-input[type=checkbox] { height: 20px !important; width: 20px !important; } .kits_form_col {padding-bottom: 45px;} .hs-richtext h4 {margin-bottom: 15px !important;} .kits_form_col label.hs-form-checkbox-display {margin-bottom: 15px;} .kits_form_col .hbspt-form li.hs-form-checkbox label span{ margin-left: 0px !important; padding-left: 31px; } .hbspt-form label.hs-form-radio-display { position: relative; padding: 4px 31px; } .hbspt-form .hs-form-radio-display span, .hbspt-form .hs-form-checkbox-display span { position: relative; cursor: pointer; font-size: 15px; font-weight: 300; } .hbspt-form .hs-input[type=radio] { position: absolute; left: 0; z-index: 2; width: 20px !important; height: 20px !important; outline: none; cursor: pointer; background-color: transparent !important; border-radius: 50%; } .hbspt-form .hs_protocol_synopsis_upload .input{position: relative;} .hbspt-form input[type="file"]::-webkit-file-upload-button { cursor: pointer; background-color: #157479; padding: 10px 20px !important; border: 1px solid #157479; border-top-left-radius: 0px; border-bottom-left-radius: 0px; border-top-right-radius: 6px; border-bottom-right-radius: 6px; color: #fff; position: absolute; top: 0; bottom: 0; right: 0; } .hbspt-form input[type="file"] { cursor: pointer; padding: 10px 20px !important; position: relative; top: 0; bottom: 0; right: 0; } /*Form Responsive CSS*/ @media only screen and (min-width: 1024px) and (max-width: 1366px){ .hs_email, .hs_contact_country { clear: both; } } @media only screen and (min-width: 992px) and (max-width: 1024px){ .hs_email, .hs_contact_country { clear: both; } } @media (min-width: 640px) and (max-width: 1120px) { .hbspt-form h1{font-size: 36px !important;} } @media screen and (min-width: 1120px) { .hbspt-form h1{font-size: 45px !important;margin-bottom: 17px !important;} .hbspt-form .gform_heading p{font-size: 17px !important;} } @media only screen and (min-width: 768px) and (max-width: 992px){ .hs_email, .hs_contact_country{ clear: both; } } @media (min-width: 767px) and (max-width: 1024px) { body .hubspot_signup_form .hs_email{width: 66% !important; float: left !important;} } @media screen and (max-width: 992px){ .assay-kit-hbspt-form .fl-col { width: 100%; } } @media screen and (max-width:812px){ .assay-kit-hbspt-form .fl-col { max-width: 100%; } .hbspt-form .hs-fieldtype-select .input:before{ right: 20px !important; } } @media screen and (max-width: 767px){ .hs_submit .actions{padding-top: 0px !important; margin-top: 0px !important;} body .hbspt-form fieldset.form-columns-2 .input {margin-right: 0px !important;} body fieldset.form-columns-3 .input{margin-right: 0px !important;} .hs-form-field .hs-input { width: 100% !important; } .hs-fieldtype-textarea .input{margin-right: 0px !important;} .hs_firstname, .hs_phone, .hs_development_area{ width: 100% !important; } } @media screen and (min-width: 640px){ .hbspt-form .hs_firstname, .kits_form_col .hs_email{ padding-right: 10px; } .hbspt-form .hs-lastname, .kits_form_col .hs_confirm_email{ padding-left: 10px; } .kits_form_col {padding-bottom: 108px;} .hs-richtext h4 {margin-bottom: 30px !important;} .kits_form_col label.hs-form-checkbox-display {margin-bottom: 20px;} } @media screen and (max-width: 640px){ .hbspt-form h1{font-size: 32px !important;} .hbspt-form .gform_heading p{font-size: 16px !important;} .hbspt-form .form-columns-2 .field{ -webkit-box-flex: 0; -ms-flex: 0 0 100%; flex: 0 0 100%; margin-bottom: .5rem; padding-left: 0; } } @media screen and (max-width: 540px){ .event-hero-section > div{ justify-items: flex-start !important; } } @media screen and (max-width: 414px){ body .hubspot_signup_form .hs_email {width: 66% !important;} body .hubspot_signup_form ul.no-list{top: 65px !important;} } @media screen and (max-width: 320px){ body .hubspot_signup_form .hs_email {width: 61% !important;} }</style> <style type="text/css">.about-presenter-section{display:none;} .space-hero-section{margin-bottom:0px !important;} .partner_bioAgilytix {padding-right: 2.5rem !important;} @media screen and (max-width: 540px){ .event-hero-section > div{ justify-items: flex-start !important; } }</style> <style type="text/css">/* Add your CSS code here. 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href="https://www.bioagilytix.com/scientific-expertise/">Scientific Expertise</a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/scientific-expertise/scientific-insights/">Scientific Insights</a></span> <span class="breadcrumb-separator"></span> <span><a href="https://www.bioagilytix.com/scientific-expertise/scientific-insights/posters/">Scientific Posters</a></span> <span class="breadcrumb-separator"></span> <span class="breadcrumb_last" aria-current="page">Validation of a Flow Cytometric Assay to Detect Antibodies to a Novel Cell Therapy for Immunogenicity Assessment</span></span></div></div></section><div class="em-news alignwide"><div class="em-news__content aligndefault"><p class=""><strong>Purpose:</strong></p><p class="">Genetically engineered cell therapies, specifically CAR (chimeric antigen receptor)-based cell therapies, are successful treatments in immune oncology. Now, alternative engineered cell-surface proteins, including cytokines, immune-evasion proteins, and other immunologic complexes show therapeutic promise. Immunogenicity to these engineered proteins is a safety concern during clinical studies. Thus, it is important to develop robust assays for measuring potential immune responses to these novel therapeutics. Herein, a cell-based, flow cytometric assay was validated to assess humoral immunogenicity to the engineered cell-surface protein in human serum. A cell-based assay was warranted, as the engineered cell-surface protein could not be purified for use in a standard ligand binding assay.</p><div class="wp-block-emagine-button gated-content__download-button em-mt--md em-mb--md" data-resource-type="file"> <a class="btn btn--size-md btn--style-primary has-primary-background-color" href="https://www.bioagilytix.com/wp-content/uploads/2024/06/Cyto-Poster-2023-Final.pdf" target="_blank" rel="noopener norefferer"> <span>Download Poster</span> </a></div><div class="pagination"><div class="nav-previous alignleft"><a href="https://www.bioagilytix.com/scientific-expertise/scientific-insights/toxicokinetics-of-cbx129801-a-bio-active-c-peptide-for-potential-replacement-therapy-in-type-1-diabetic-peripheralneuropathy-following-subcutaneous-injection-of-cbx129801-in-rats-in-a-6-month-study/" rel="prev">Toxicokinetics of CBX129801, a Bio active C peptide for Potential Replacement Therapy in Type 1 Diabetic PeripheralNeuropathy, Following Subcutaneous Injection of CBX129801 in Rats in a 6-Month Study with a 7-Week Recovery Period</a></div><div class="nav-next alignright"></div></div></div><div class="em-news--image-container"><div class="em-news__media"><div class="em-news__media-inner"> <img src="https://www.bioagilytix.com/wp-content/uploads/2024/06/Screen_Shot_2024-06-17_at_6.33.09_PM-removebg-preview.png" alt="" class="object-fit--cover" srcset="https://www.bioagilytix.com/wp-content/uploads/2024/06/Screen_Shot_2024-06-17_at_6.33.09_PM-removebg-preview.png 500w, https://www.bioagilytix.com/wp-content/uploads/2024/06/Screen_Shot_2024-06-17_at_6.33.09_PM-removebg-preview-300x300.png 300w, https://www.bioagilytix.com/wp-content/uploads/2024/06/Screen_Shot_2024-06-17_at_6.33.09_PM-removebg-preview-150x150.png 150w, https://www.bioagilytix.com/wp-content/uploads/2024/06/Screen_Shot_2024-06-17_at_6.33.09_PM-removebg-preview-100x100.png 100w" width="500" height="500"></div></div></div></div></article></main><footer 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