CINXE.COM

NHP Luminex XL Cytokine Performance Premixed Kit (FCSTM21): R&D Systems

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r.ee.get(this.agentIdentifier).abort(),!1}}}({features:[R,_,_e,Ne,he,N,L,Pe,ke],loaderType:"pro"})})()})();</script> <link rel="profile" href="http://www.w3.org/1999/xhtml/vocab" /> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <meta name="keywords" content="nhp luminex" /> <link rel="canonical" href="https://www.rndsystems.com/products/nhp-xl-cytokine-luminex-performance-premixed-kit_fcstm21" /> <meta refresh_date="11/22/2024 11:55:01 PM" /> <title>NHP Luminex XL Cytokine Performance Premixed Kit (FCSTM21): R&D Systems</title> <meta name="description" content="Build your NHP Luminex Assay. 36 targets available. Tested for sensitivity, intra-assay precision, inter-assay precision, recovery, and linearity." /> <meta http-equiv="content-language" content="en-us" /> <link href="https://www.rndsystems.com/products/nhp-xl-cytokine-luminex-performance-premixed-kit_fcstm21" rel="alternate" hreflang="x-default" /> <link href="https://www.rndsystems.com/products/nhp-xl-cytokine-luminex-performance-premixed-kit_fcstm21" rel="alternate" hreflang="en" /> <link href="https://www.rndsystems.com/cn/products/nhp-xl-cytokine-luminex-performance-premixed-kit_fcstm21" rel="alternate" hreflang="zh-cn" /> <meta name="baidu-site-verification" content="NoHNFT2tiZ" /> <script id="json-ld" type="application/ld+json">[{"@context":"http://schema.org","@type":"Product","aggregateRating":{"@type":"AggregateRating","ratingValue":4.7000000000000002,"reviewCount":3},"offers":[],"url":"https://www.rndsystems.com/products/nhp-xl-cytokine-luminex-performance-premixed-kit_fcstm21","name":"NHP XL Cytokine Luminex\u00ae Performance Premixed Kit","productID":"FCSTM21","mpn":"FCSTM21","sku":"FCSTM21","brand":{"@type":"Brand","name":"R&D Systems"},"itemcondition":"new","image":["https://resources.rndsystems.com/images/datasheets/fcstm21_non-human-primate-xl-disc-premixed-luminex-perf-assay-kit-189202085348.png","https://resources.rndsystems.com/images/datasheets/fcstm21_non-human-primate-xl-disc-premixed-luminex-perf-assay-kit-2872020163247.jpg"],"review":[],"description":"<div class=\"col-xs-12\">\r\n<div class=\"\">\r\n<h3>Precision</h3>\r\n</div>\r\n\r\n<table class=\"table table-bordered table-striped\">\r\n\t<thead>\r\n\t\t<tr>\r\n\t\t\t<td align=\"left\" colspan=\"3\">\r\n\t\t\t<p>Intra-Assay Precision - is generated from the mean of the %CV&#39;s from 40 reportable results across two different concentrations of analytes in a single serum assay</p>\r\n\r\n\t\t\t<p>Inter-Assay Precision - is generated from the mean of the %CV&#39;s across two different concentrations of analytes across 31&nbsp;different serum assays.</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<th>&nbsp;</th>\r\n\t\t\t<th>n=40 (20x2 levels)</th>\r\n\t\t\t<th>n=31</th>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<th>Analyte</th>\r\n\t\t\t<th>Intra-Assay (%CV)</th>\r\n\t\t\t<th>Inter-Assay (%CV)</th>\r\n\t\t</tr>\r\n\t</thead>\r\n\t<tbody>\r\n\t\t<tr>\r\n\t\t\t<td>BDNF</td>\r\n\t\t\t<td>8.16</td>\r\n\t\t\t<td>13.3</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CCL2/MCP-1</td>\r\n\t\t\t<td>3.02</td>\r\n\t\t\t<td>10.5</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CCL5/RANTES</td>\r\n\t\t\t<td>3.72</td>\r\n\t\t\t<td>17.0</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CCL11/Eotaxin</td>\r\n\t\t\t<td>7.98</td>\r\n\t\t\t<td>15.4</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CCL20/MIP-3 alpha</td>\r\n\t\t\t<td>8.41</td>\r\n\t\t\t<td>17.3</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CD40 Ligand</td>\r\n\t\t\t<td>9.30</td>\r\n\t\t\t<td>15.0</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CXCL2/GRO beta</td>\r\n\t\t\t<td>7.76</td>\r\n\t\t\t<td>13.1</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CXCL10/IP-10</td>\r\n\t\t\t<td>2.95</td>\r\n\t\t\t<td>12.2</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CXCL11/I-TAC</td>\r\n\t\t\t<td>6.23</td>\r\n\t\t\t<td>13.7</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CXCL13/BLC</td>\r\n\t\t\t<td>5.79</td>\r\n\t\t\t<td>12.5</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>FGF basic</td>\r\n\t\t\t<td>5.60</td>\r\n\t\t\t<td>13.1</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>G-CSF</td>\r\n\t\t\t<td>5.55</td>\r\n\t\t\t<td>14.2</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>GM-CSF</td>\r\n\t\t\t<td>7.11</td>\r\n\t\t\t<td>14.1</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>Granzyme B</td>\r\n\t\t\t<td>9.76</td>\r\n\t\t\t<td>18.6</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IFN-alpha</td>\r\n\t\t\t<td>5.17</td>\r\n\t\t\t<td>12.4</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IFN-beta</td>\r\n\t\t\t<td>10.9</td>\r\n\t\t\t<td>15.2</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IFN-gamma</td>\r\n\t\t\t<td>6.36</td>\r\n\t\t\t<td>13.0</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-1 beta</td>\r\n\t\t\t<td>2.55</td>\r\n\t\t\t<td>12.7</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-10</td>\r\n\t\t\t<td>8.55</td>\r\n\t\t\t<td>14.1</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-12 p70</td>\r\n\t\t\t<td>4.92</td>\r\n\t\t\t<td>17.7</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-13</td>\r\n\t\t\t<td>7.97</td>\r\n\t\t\t<td>17.5</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-15</td>\r\n\t\t\t<td>4.99</td>\r\n\t\t\t<td>18.2</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-17A</td>\r\n\t\t\t<td>4.38</td>\r\n\t\t\t<td>19.0</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-2</td>\r\n\t\t\t<td>5.32</td>\r\n\t\t\t<td>18.1</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-21</td>\r\n\t\t\t<td>5.84</td>\r\n\t\t\t<td>19.0</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-4</td>\r\n\t\t\t<td>4.92</td>\r\n\t\t\t<td>17.5</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-5</td>\r\n\t\t\t<td>4.14</td>\r\n\t\t\t<td>16.5</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-6</td>\r\n\t\t\t<td>6.80</td>\r\n\t\t\t<td>17.8</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-7</td>\r\n\t\t\t<td>6.57</td>\r\n\t\t\t<td>17.7</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-8/CXCL8</td>\r\n\t\t\t<td>6.87</td>\r\n\t\t\t<td>17.5</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>PDGF-AA</td>\r\n\t\t\t<td>6.52</td>\r\n\t\t\t<td>25.0</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>PDGF-BB</td>\r\n\t\t\t<td>3.37</td>\r\n\t\t\t<td>16.9</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>PD-L1</td>\r\n\t\t\t<td>8.36</td>\r\n\t\t\t<td>19.4</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>TGF-alpha</td>\r\n\t\t\t<td>5.43</td>\r\n\t\t\t<td>18.7</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>TNF-alpha</td>\r\n\t\t\t<td>3.68</td>\r\n\t\t\t<td>17.2</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>VEGF</td>\r\n\t\t\t<td>4.79</td>\r\n\t\t\t<td>18.4</td>\r\n\t\t</tr>\r\n\t</tbody>\r\n</table>\r\n</div>\r\n\r\n<div class=\"col-xs-12\">\r\n<div class=\"\">\r\n<h3>Accuracy</h3>\r\n</div>\r\n\r\n<table class=\"table table-bordered table-striped\">\r\n\t<thead>\r\n\t\t<tr>\r\n\t\t\t<td colspan=\"3\">\r\n\t\t\t<p>Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk.</p>\r\n\r\n\t\t\t<p>Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.</p>\r\n </td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<th>&nbsp;</th>\r\n\t\t\t<th align=\"center\" colspan=\"1\">Linearity</th>\r\n\t\t\t<th align=\"center\" colspan=\"1\">Spiked<br />\r\n\t\t\tRecovery</th>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<th>Analyte</th>\r\n\t\t\t<th>Average %</th>\r\n\t\t\t<th>Average %</th>\r\n\t\t</tr>\r\n\t</thead>\r\n\t<tfoot>\r\n\t</tfoot>\r\n\t<tbody>\r\n\t\t<tr>\r\n\t\t\t<td>BDNF</td>\r\n\t\t\t<td>114</td>\r\n\t\t\t<td>HE</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CCL2/MCP-1</td>\r\n\t\t\t<td>75</td>\r\n\t\t\t<td>135</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CCL5/RANTES</td>\r\n\t\t\t<td>98</td>\r\n\t\t\t<td>HE</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CCL11/Eotaxin</td>\r\n\t\t\t<td>93</td>\r\n\t\t\t<td>138</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CCL20/MIP-3 alpha</td>\r\n\t\t\t<td>105</td>\r\n\t\t\t<td>111</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CD40 Ligand</td>\r\n\t\t\t<td>73</td>\r\n\t\t\t<td>105</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CXCL2/GRO beta</td>\r\n\t\t\t<td>95</td>\r\n\t\t\t<td>81</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CXCL10/IP-10</td>\r\n\t\t\t<td>78</td>\r\n\t\t\t<td>72</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CXCL11/I-TAC</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>97</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>CXCL13/BLC</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>114</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>FGF basic</td>\r\n\t\t\t<td colspan=\"2\" rowspan=\"1\">NV</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>G-CSF</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>67</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>GM-CSF</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>111</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>Granzyme B</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>122</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IFN-alpha</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>118</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IFN-beta</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>71</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IFN-gamma</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>124</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-1 beta</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>68</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-10</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>104</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-12 p70</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>109</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-13</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>118</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-15</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>90</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-17A</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>90</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-2</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>89</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-21</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>121</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-4</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>80</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-5</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>95</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-6</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>84</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-7</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>97</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>IL-8/CXCL8</td>\r\n\t\t\t<td>121</td>\r\n\t\t\t<td>HE</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>PDGF-AA</td>\r\n\t\t\t<td>101</td>\r\n\t\t\t<td>99</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>PDGF-BB</td>\r\n\t\t\t<td>103</td>\r\n\t\t\t<td>HE</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>PD-L1</td>\r\n\t\t\t<td>80</td>\r\n\t\t\t<td>HE</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>TGF-alpha</td>\r\n\t\t\t<td colspan=\"2\" rowspan=\"1\">NV</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>TNF-alpha</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>85</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td>VEGF</td>\r\n\t\t\t<td>NO</td>\r\n\t\t\t<td>97</td>\r\n\t\t</tr>\r\n\t</tbody>\r\n</table>\r\n</div>","additionalProperty":[{"@type":"PropertyValue","name":"Species","value":"Primate"},{"@type":"PropertyValue","name":"Assay Type","value":"Magnetic bead-based multiplex assay for the Luminex&#174; platform"}]},{"@context":"http://schema.org","@type":"Question","name":"Which primate species have been tested with the NHP XL Cytokine Premixed Kit?","acceptedAnswer":{"@type":"Answer","text":"The kit has been tested and validated with Cynomolgus monkey and Rhesus macaque samples. The kit is broadly designated as reactive with non-human primate samples due to the expected high homology across primate species."}},{"@context":"http://schema.org","@type":"WebPage","breadcrumb":{"@context":"http://schema.org","@type":"BreadcrumbList","itemListElement":[{"@context":"http://schema.org","@type":"ListItem","position":1,"item":{"@context":"http://schema.org","@type":"Thing","@id":"https://www.rndsystems.com/","name":"Home"}},{"@context":"http://schema.org","@type":"ListItem","position":2,"item":{"@context":"http://schema.org","@type":"Thing","@id":"https://www.rndsystems.com/target/multiplex-cytokine-assay-kits","name":"Multiplex Cytokine Assay Kits"}},{"@context":"http://schema.org","@type":"ListItem","position":3,"item":{"@context":"http://schema.org","@type":"Thing","@id":"https://www.rndsystems.com/products/nhp-xl-cytokine-luminex-performance-premixed-kit_fcstm21","name":"NHP XL Cytokine Luminex\u00ae Performance Premixed Kit"}}]},"potentialaction":{"query":""}}]</script> <meta name="generator" content="Drupal 7 (https://www.drupal.org)" /> <link rel="shortlink" href="https://www.rndsystems.com/products/nhp-xl-cytokine-luminex-performance-premixed-kit_fcstm21" /> <meta property="og:site_name" content="www.rndsystems.com" /> <meta property="og:type" content="article" /> <meta property="og:url" content="https://www.rndsystems.com/products/nhp-xl-cytokine-luminex-performance-premixed-kit_fcstm21" /> <meta property="og:title" content="NHP XL Cytokine Luminex® Performance Premixed Kit" /> <meta property="og:image" content="https://resources.rndsystems.com/images/Social/2019RnDSystemslogoV.png" /> <style> @import url("https://www.rndsystems.com/sites/rndsystems.com/modules/jquery_update/replace/ui/themes/base/minified/jquery.ui.core.min.css?snds8q"); @import url("https://www.rndsystems.com/sites/rndsystems.com/modules/jquery_update/replace/ui/themes/base/minified/jquery.ui.theme.min.css?snds8q"); @import 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<a id="main-content"></a> <div class="region region-content"> <section id="block-system-main" class="block block-system clearfix"> <div class="row"> <div id="content" class="col-xs-12 col-sm-8 col-md-9 col-lg-9 col-xl-9"> <div id="content_column"> <div class="ds_title_table"> <h1 class="ds_title">NHP XL Cytokine Luminex® Performance Premixed Kit </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: FCSTM21</span> <br/> <span class="citations"> <a href="#product-citations" id="citation_count" class="jump_to" data-tab="3" data-tabname="product-citations"> 4 Citations </a> </span> <span class="reviews review_stars_5"> <a href="#product-reviews" id="product_reviews" class="jump_to" data-tab="6" data-tabname="product-reviews"> 3 Reviews </a> </span> <a href="/reviews/FCSTM21" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div> <!--<div class="formulations_row row"> <div class="formulations_wrapper col-xs-12"> </div> </div>--> <div class="product_wrapper row"> <div class="datasheet_luminex_availability_container col-xs-12 col-md-8 col-md-push-4"> <p class="datasheet_luminex_availability_text"> Build your own Luminex Assay with our Luminex Assay Customization Tool. </p> <p class="datasheet_luminex_availability"> U.S. orders usually ship in 5-10 business days </p> <div style="margin-bottom: 10px;"></div> <a href="#luminex_analyte_page" class="btn btn-primary">Build Now</a> </div> <div class="product_image_wrapper col-xs-12 col-md-4 col-md-pull-8"> <div class="bestseller_product"><span>Best Seller</span></div> <div class="product_image"> <span class="product_image_label"> </span> <a class="ds-image overview-images" data-toggle="lightbox" data-gallery="overview-images" onClick="event.preventDefault();" data-title="NHP Luminex Standard Curves (Cell Culture Supernates)" data-footer="NHP Luminex Standard Curves (Cell Culture Supernates)" title="Cat. No. FCSTM21 - NHP XL Cytokine Luminex&reg; Performance Premixed Kit" href="https://resources.rndsystems.com/images/datasheets/fcstm21_non-human-primate-xl-disc-premixed-luminex-perf-assay-kit-189202085348.png"> <img fetchpriority="high" src="https://resources.rndsystems.com/images/datasheets/fcstm21_non-human-primate-xl-disc-premixed-luminex-perf-assay-kit-189202085348.png" class="img-fluid" alt="NHP Luminex Standard Curves (Cell Culture Supernates)" title="NHP Luminex Standard Curves (Cell Culture Supernates)"> </a> <div class="search_image_other_count"> <span class="search_more_images">2 Images</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> <a class="ds-image" data-toggle="lightbox" data-gallery="overview-images" data-footer="NHP Luminex XL Cytokine Luminex Performance Premixed Kit" data-title="NHP Luminex XL Cytokine Luminex Performance Premixed Kit" title="Cat. No. FCSTM21 - NHP XL Cytokine Luminex&reg; Performance Premixed Kit" onClick="event.preventDefault();" href="https://resources.rndsystems.com/images/datasheets/fcstm21_non-human-primate-xl-disc-premixed-luminex-perf-assay-kit-2872020163247.jpg"> <img src="https://resources.rndsystems.com/images/datasheets/fcstm21_non-human-primate-xl-disc-premixed-luminex-perf-assay-kit-2872020163247.jpg" class="img-fluid" loading="lazy" alt="NHP Luminex XL Cytokine Luminex Performance Premixed Kit" title="NHP Luminex XL Cytokine Luminex Performance Premixed Kit"> </a> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/multiplex-cytokine-assay-kits?category=Luminex Assays" class="btn btn-secondary">All Multiplex Cytokine Assay Kits Luminex Assays</a> </div> </div> <div class="tabbed_content" id="rnd_luminex"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_3" data-tab="3" data-tabname="product-citations" id="dsTabs_product-citations"> <span>Citations (4)</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews (3) </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#luminex_analyte_page" id="product-anchor-luminex_analyte_page">Configure Your Assay</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#luminex" id="product-anchor-luminex">Product Features</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#luminex_accuracy_and_precision" id="product-anchor-luminex_accuracy_and_precision">Precision & Accuracy</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> </div> <div id="ds_summary" class="datasheet_section"> <h2 class="datasheet-product-title">NHP XL Cytokine Luminex® Performance Premixed Kit Summary</h2> <table class="technical_data_table"> <tbody> <tr> <td class="tdt_name">Panel</td> <td class="tdt_data">Luminex Performance NHP XL Cytokine Panel</td> </tr> <tr> <td class="tdt_name">Assay Type</td> <td class="tdt_data">Magnetic bead-based multiplex assay for the Luminex<sup>&#174;</sup> platform</td> </tr> <tr> <td class="tdt_name">Format</td> <td class="tdt_data">1 x 96-well microplate and Magnetic antibody-coated beads</td> </tr> <tr> <td class="tdt_name">Species</td> <td class="tdt_data">Primate</td> </tr> <tr> <td class="tdt_name">Analytes Detected</td> <td class="tdt_data">Please see analyte list in assay customization tool below.</td> </tr> <tr> <td class="tdt_name">Performance Validation</td> <td class="tdt_data">Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.<br/><br/>View <a target="_blank" href="https://www.rndsystems.com/products/luminex-performance-non-human-primate-xl-cytokine-panel">Luminex High Performance Non-human Primate XL validation data</a> on rndsystems.com.</td> </tr> </tbody> </table> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="FCSTM21"></div> <div id="luminex_analyte_page" class="page-container luminex-analyte-page"> <div class="row"> <div class="luminex-whole-column col-xs-12 col-sm-12"> <h2 class="luminex-analytes-summary">Make your selections below to configure your assay</h2> <div class="luminex-analytes-filter-container luminex-hide-on-load"> <div class="luminex-analytes-filter-element-wrapper"> <span class="luminex-analytes-filter-element luminex-analytes-filter-label">Filter Analytes</span> <span class="luminex-analytes-filter-element luminex-analytes-filter-input-area"> <input type="text" class="luminex-analytes-filter-input" id="luminex_input_filter_results" placeholder="Analyte"/> <span class="luminex-button luminex-button-filter-clear luminex-hide-on-load" id="luminex_button_filter_clear"></span> </span> <!-- end .luminex-analytes-filter-input-area --> </div> <!-- end .luminex-analytes-filter-element-wrapper --> <div class="luminex-analytes-select-wrapper"> <span class="luminex-analytes-filter-element luminex-analytes-select-all-container luminex-button-wrapper"> <span class="btn btn-quinary luminex-button luminex-button-select-all" id="luminex_button_select_all">Select All</span> </span> <!-- end .luminex-analytes-select-all-container --> <span class="luminex-analytes-filter-element luminex-analytes-select-none-container luminex-button-wrapper"> <span class="btn btn-quinary luminex-button luminex-button-select-none" id="luminex_button_select_none">Select None</span> </span> <!-- end .luminex-analytes-select-all-container --> </div> <!-- end .luminex-analytes-select-wrapper --> </div> <!-- end .luminex-analytes-filter-container --> <div class="luminex-analytes-results row" id="luminex_analytes_results"> </div> <!-- end .luminex-analytes-results --> <div class="luminex-assays-section luminex-right-side luminex-hide-on-load"> <div class="row"> <div class="luminex-kits-header col-xs-12"> <h2 class="luminex-kits-summary">Assays</h2> <p class="luminex-kits-instructions"> Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed. </p> <p><b>If you run into bead region incompatibilities, <a href="https://www.rndsystems.com/services/luminex-assays-custom-development-services" target="_blank">send your Luminex code</a> to our team and find out more.</b></p> </div> </div> <div class="luminex-splitting-area row"> <div class="luminex-splitting-mixing luminex-splitting-box col-xs-12 col-sm-6" id="luminex_splitting_mixing"> <div class="luminex-splitting-row luminex-splitting-row-header"> <h4 class="luminex-mixing-question luminex-splitting-question">How would you like these analytes mixed?</h4> </div> <!-- end .luminex-splitting-row-header --> <div class="luminex-splitting-row luminex-splitting-row-premixed"> <div class="luminex-splitting-row-select-premixed luminex-splitting-row-select-mixing luminex-splitting-row-select luminex-mixing-selected" data-mixing="premixed">Premixed<span class="mixing-info-icon structure-info-icon info-icon luminex-info-icon-mixing luminex-info-icon-premixed" data-placement="auto left" data-title="Beads and biotin antibodies are mixed before shipment" data-group="luminex-info-icon-mixing" data-name="luminex-info-icon-premixed"></span></div> </div> <!-- end .luminex-splitting-row-premixed --> <div class="luminex-splitting-row luminex-splitting-row-user-mixed"> <div class="luminex-splitting-row-select-user-mixed luminex-splitting-row-select-mixing luminex-splitting-row-select" data-mixing="user-mixed">User Mixed<span class="mixing-info-icon structure-info-icon info-icon luminex-info-icon-mixing luminex-info-icon-user-mixed" data-placement="auto left" data-title="Mix your own beads and biotin antibodies when your need them" data-group="luminex-info-icon-mixing" data-name="luminex-info-icon-user-mixed"></span></div> </div> <!-- end .luminex-splitting-row-user-mixed --> <div class="luminex-splitting-row-alert luminex-hide-on-load"> <div class="luminex-splitting-row-alert-content alert alert-warning">Please select at least one mixing option to see the kits.</div> </div> <!-- end .luminex-splitting-row-user-mixed --> </div> <!-- end .luminex-splitting-mixing --> <div class="luminex-splitting-dilution luminex-splitting-box col-xs-12 col-sm-6" id="luminex_splitting_dilution"> <div class="luminex-splitting-row luminex-splitting-row-header"> <h4 class="luminex-dilution-question luminex-splitting-question">Would you like to split based on dilution?</h4> </div> <!-- end .luminex-splitting-row-header --> <div class="luminex-splitting-row luminex-splitting-row-do-not-split"> <div class="luminex-splitting-row-select-do-not-split luminex-splitting-row-select-dilution luminex-splitting-row-select luminex-dilution-selected" data-dilution="do-not-split">Do not split<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-" data-placement="auto left" data-title="Different sample type dilutions are not considered" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-do-not-split"></span></div> </div> <!-- end .luminex-splitting-row-do-not-split --> <div class="luminex-splitting-row luminex-splitting-row-split-serum"> <div class="luminex-splitting-row-select-split-serum luminex-splitting-row-select-dilution luminex-splitting-row-select" data-dilution="split-serum">Split based on serum<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-serum" data-placement="auto left" data-title="Panel is split based upon matching serum sample dilutions" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-serum"></span></div> </div> <!-- end .luminex-splitting-row-split-serum --> <div class="luminex-splitting-row luminex-splitting-row-split-plasma"> <div class="luminex-splitting-row-select-split-plasma luminex-splitting-row-select-dilution luminex-splitting-row-select" data-dilution="split-plasma">Split based on plasma<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-plasma" data-placement="auto left" data-title="Panel is split based upon matching plasma sample dilutions" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-plasma"></span></div> </div> <!-- end .luminex-splitting-row-split-plasma --> <div class="luminex-splitting-row luminex-splitting-row-split-cell-culture"> <div class="luminex-splitting-row-select-split-cell-culture luminex-splitting-row-select-dilution luminex-splitting-row-select" data-dilution="split-cell-culture">Split based on cell culture supernates<span class="dilution-info-icon structure-info-icon info-icon luminex-info-icon-dilution luminex-info-icon-cell-culture" data-placement="auto left" data-title="Panel is split based upon matching cell culture supernate sample dilutions" data-group="luminex-info-icon-dilution" data-name="luminex-info-icon-cell-culture"></span></div> </div> <!-- end .luminex-splitting-row-split-cell-culture --> </div> <!-- end .luminex-splitting-dilution --> </div> <!-- end .luminex-splitting-area --> <div class="luminex-kits-area row" id="luminex_kits_area"> </div> <!-- end .luminex-kits-area --> </div> <!-- end .luminex-assays-section .luminex-right-side .luminex-hide-on-load --> </div> <!-- end .luminex-whole-column --> </div> <!-- end .row --> </div> <!-- end #luminex_analyte_page --> <div id="luminex" class="datasheet_section"><div class="datasheet-luminex-content"> <p class="luminex-text"> Not finding your analyte of interest? View our complete Luminex offering in the full version of our <a href="/luminex/analytes">Luminex Assay Customization Tool</a>. We also offer <a href="/services/luminex-assays-custom-development-services">Custom Luminex Services</a>. </p> <div class="luminex-text-group luminex-product-features"> <div class="ds_lightBlueBar">Product Features</div> <p class="luminex-text"> Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples. </p> <ul class="luminex-list luminex-product-features-list"> <li class="luminex-list-item">Simultaneously profile up to <span class="luminex-analytes-displayed"></span> analytes of your choice in one sample</li> <li class="luminex-list-item">Magnetic format allows for easier wash steps</li> <li class="luminex-list-item">Undergoes similar validation testing as Quantikine<sup>&#174;</sup> ELISA Kits</li> <li class="luminex-list-item">Precise and Specific</li> <li class="luminex-list-item">Requires a small sample volume (&lt;50 &#181;L)</li> <li class="luminex-list-item">Run your assay in just 3-5 hours</li> <li class="luminex-list-item">Mass-calibrated standards for consistent results with every new lot of material</li> <li class="luminex-list-item">Enough reagents for one 96-well plate</li> </ul> <!-- end .luminex-product-features-list --> </div> <!-- end .luminex-product-features --> <div class="luminex-text-group luminex-kit-components"> <div class="ds_lightBlueBar"> Kit Components </div> <ul class="luminex-list luminex-kit-components-list"> <li class="luminex-list-item">Premixed cocktail of antibody-coated Magnetic beads</li> <li class="luminex-list-item">Premixed cocktail of biotinylated detection antibody</li> <li class="luminex-list-item">Standard Cocktail(s)</li> <li class="luminex-list-item">Bead Diluent</li> <li class="luminex-list-item">Biotin Antibody Diluent</li> <li class="luminex-list-item">Calibrator Diluent</li> <li class="luminex-list-item">Wash Buffer</li> <li class="luminex-list-item">Streptavidin-PE</li> <li class="luminex-list-item">One flat-bottom 96-well Microplate</li> <li class="luminex-list-item">Foil Plate Sealers (4)</li> <li class="luminex-list-item">Mixing Bottle</li> <li class="luminex-list-item">Standard Value Card**</li> <li class="luminex-list-item">High and low controls with Control Mean Value Card</li> </ul> <!-- end .luminex-kit-components-list --> <p class="luminex-text luminex-note"> **A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit. </p> </div> <!-- end .luminex-kit-components --> <div id="luminex_accuracy_and_precision"> <div class="col-xs-12"> <div class=""> <h3>Precision</h3> </div> <table class="table table-bordered table-striped"> <thead> <tr> <td align="left" colspan="3"> <p>Intra-Assay Precision - is generated from the mean of the %CV&#39;s from 40 reportable results across two different concentrations of analytes in a single serum assay</p> <p>Inter-Assay Precision - is generated from the mean of the %CV&#39;s across two different concentrations of analytes across 31&nbsp;different serum assays.</p> </td> </tr> <tr> <th>&nbsp;</th> <th>n=40 (20x2 levels)</th> <th>n=31</th> </tr> <tr> <th>Analyte</th> <th>Intra-Assay (%CV)</th> <th>Inter-Assay (%CV)</th> </tr> </thead> <tbody> <tr> <td>BDNF</td> <td>8.16</td> <td>13.3</td> </tr> <tr> <td>CCL2/MCP-1</td> <td>3.02</td> <td>10.5</td> </tr> <tr> <td>CCL5/RANTES</td> <td>3.72</td> <td>17.0</td> </tr> <tr> <td>CCL11/Eotaxin</td> <td>7.98</td> <td>15.4</td> </tr> <tr> <td>CCL20/MIP-3 alpha</td> <td>8.41</td> <td>17.3</td> </tr> <tr> <td>CD40 Ligand</td> <td>9.30</td> <td>15.0</td> </tr> <tr> <td>CXCL2/GRO beta</td> <td>7.76</td> <td>13.1</td> </tr> <tr> <td>CXCL10/IP-10</td> <td>2.95</td> <td>12.2</td> </tr> <tr> <td>CXCL11/I-TAC</td> <td>6.23</td> <td>13.7</td> </tr> <tr> <td>CXCL13/BLC</td> <td>5.79</td> <td>12.5</td> </tr> <tr> <td>FGF basic</td> <td>5.60</td> <td>13.1</td> </tr> <tr> <td>G-CSF</td> <td>5.55</td> <td>14.2</td> </tr> <tr> <td>GM-CSF</td> <td>7.11</td> <td>14.1</td> </tr> <tr> <td>Granzyme B</td> <td>9.76</td> <td>18.6</td> </tr> <tr> <td>IFN-alpha</td> <td>5.17</td> <td>12.4</td> </tr> <tr> <td>IFN-beta</td> <td>10.9</td> <td>15.2</td> </tr> <tr> <td>IFN-gamma</td> <td>6.36</td> <td>13.0</td> </tr> <tr> <td>IL-1 beta</td> <td>2.55</td> <td>12.7</td> </tr> <tr> <td>IL-10</td> <td>8.55</td> <td>14.1</td> </tr> <tr> <td>IL-12 p70</td> <td>4.92</td> <td>17.7</td> </tr> <tr> <td>IL-13</td> <td>7.97</td> <td>17.5</td> </tr> <tr> <td>IL-15</td> <td>4.99</td> <td>18.2</td> </tr> <tr> <td>IL-17A</td> <td>4.38</td> <td>19.0</td> </tr> <tr> <td>IL-2</td> <td>5.32</td> <td>18.1</td> </tr> <tr> <td>IL-21</td> <td>5.84</td> <td>19.0</td> </tr> <tr> <td>IL-4</td> <td>4.92</td> <td>17.5</td> </tr> <tr> <td>IL-5</td> <td>4.14</td> <td>16.5</td> </tr> <tr> <td>IL-6</td> <td>6.80</td> <td>17.8</td> </tr> <tr> <td>IL-7</td> <td>6.57</td> <td>17.7</td> </tr> <tr> <td>IL-8/CXCL8</td> <td>6.87</td> <td>17.5</td> </tr> <tr> <td>PDGF-AA</td> <td>6.52</td> <td>25.0</td> </tr> <tr> <td>PDGF-BB</td> <td>3.37</td> <td>16.9</td> </tr> <tr> <td>PD-L1</td> <td>8.36</td> <td>19.4</td> </tr> <tr> <td>TGF-alpha</td> <td>5.43</td> <td>18.7</td> </tr> <tr> <td>TNF-alpha</td> <td>3.68</td> <td>17.2</td> </tr> <tr> <td>VEGF</td> <td>4.79</td> <td>18.4</td> </tr> </tbody> </table> </div> <div class="col-xs-12"> <div class=""> <h3>Accuracy</h3> </div> <table class="table table-bordered table-striped"> <thead> <tr> <td colspan="3"> <p>Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk.</p> <p>Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.</p> </td> </tr> <tr> <th>&nbsp;</th> <th align="center" colspan="1">Linearity</th> <th align="center" colspan="1">Spiked<br /> Recovery</th> </tr> <tr> <th>Analyte</th> <th>Average %</th> <th>Average %</th> </tr> </thead> <tfoot> </tfoot> <tbody> <tr> <td>BDNF</td> <td>114</td> <td>HE</td> </tr> <tr> <td>CCL2/MCP-1</td> <td>75</td> <td>135</td> </tr> <tr> <td>CCL5/RANTES</td> <td>98</td> <td>HE</td> </tr> <tr> <td>CCL11/Eotaxin</td> <td>93</td> <td>138</td> </tr> <tr> <td>CCL20/MIP-3 alpha</td> <td>105</td> <td>111</td> </tr> <tr> <td>CD40 Ligand</td> <td>73</td> <td>105</td> </tr> <tr> <td>CXCL2/GRO beta</td> <td>95</td> <td>81</td> </tr> <tr> <td>CXCL10/IP-10</td> <td>78</td> <td>72</td> </tr> <tr> <td>CXCL11/I-TAC</td> <td>NO</td> <td>97</td> </tr> <tr> <td>CXCL13/BLC</td> <td>NO</td> <td>114</td> </tr> <tr> <td>FGF basic</td> <td colspan="2" rowspan="1">NV</td> </tr> <tr> <td>G-CSF</td> <td>NO</td> <td>67</td> </tr> <tr> <td>GM-CSF</td> <td>NO</td> <td>111</td> </tr> <tr> <td>Granzyme B</td> <td>NO</td> <td>122</td> </tr> <tr> <td>IFN-alpha</td> <td>NO</td> <td>118</td> </tr> <tr> <td>IFN-beta</td> <td>NO</td> <td>71</td> </tr> <tr> <td>IFN-gamma</td> <td>NO</td> <td>124</td> </tr> <tr> <td>IL-1 beta</td> <td>NO</td> <td>68</td> </tr> <tr> <td>IL-10</td> <td>NO</td> <td>104</td> </tr> <tr> <td>IL-12 p70</td> <td>NO</td> <td>109</td> </tr> <tr> <td>IL-13</td> <td>NO</td> <td>118</td> </tr> <tr> <td>IL-15</td> <td>NO</td> <td>90</td> </tr> <tr> <td>IL-17A</td> <td>NO</td> <td>90</td> </tr> <tr> <td>IL-2</td> <td>NO</td> <td>89</td> </tr> <tr> <td>IL-21</td> <td>NO</td> <td>121</td> </tr> <tr> <td>IL-4</td> <td>NO</td> <td>80</td> </tr> <tr> <td>IL-5</td> <td>NO</td> <td>95</td> </tr> <tr> <td>IL-6</td> <td>NO</td> <td>84</td> </tr> <tr> <td>IL-7</td> <td>NO</td> <td>97</td> </tr> <tr> <td>IL-8/CXCL8</td> <td>121</td> <td>HE</td> </tr> <tr> <td>PDGF-AA</td> <td>101</td> <td>99</td> </tr> <tr> <td>PDGF-BB</td> <td>103</td> <td>HE</td> </tr> <tr> <td>PD-L1</td> <td>80</td> <td>HE</td> </tr> <tr> <td>TGF-alpha</td> <td colspan="2" rowspan="1">NV</td> </tr> <tr> <td>TNF-alpha</td> <td>NO</td> <td>85</td> </tr> <tr> <td>VEGF</td> <td>NO</td> <td>97</td> </tr> </tbody> </table> </div> </div> <div class="luminex-text-group luminex-right-for-me"> <div class="ds_lightBlueBar"> Which Luminex Assay is right for me? </div> <p class="luminex-text"> Assays for the Luminex platform are offered as <a href="/products/luminex-high-performance-assays">High Performance Assays</a> or <a href="/products/luminex-assays">Assays</a>. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. <a href="/products/which-luminex-assay-right-me">View a table comparing the features and benefits of these bead-based multiplex assays.</a> </p> </div> <!-- end .luminex-right-for-me --> <div class="luminex-text-group luminex-more-information"> <div class="ds_lightBlueBar"> More Information About Magnetic Bead-based Multiplex Kits For The Luminex<sup>&#174;</sup> Platform </div> <p class="luminex-text"> Magnetic <a href="/products/luminex-high-performance-assays">Luminex High Performance Assays</a> are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the <a href="/products/luminex-200-ruo-system-w-xponent-42_lx200-xpon-ruo">Luminex 100/200&#8482;</a> or <a href="/products/flexmap-3d-with-xponent-42_flexmap-3d-ruo">FLEXMAP 3D</a>, dual laser, flow-based sorting and detection platforms. </p> <p class="luminex-text"> Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems. </p> </div> <!-- end .luminex-more-information --> </div> <!-- end .datasheet-luminex-content --> </div> <div id="ds_scientific_data" class="datasheet_section"> <div id="ds_data_examples"> <h2>Scientific Data</h2> <div id="data_examples"> <div class="row data_ex_row"> <a id="ds_image_0" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> Multiplex Immunoassay </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-0" data-title="NHP Luminex Standard Curves (Cell Culture Supernates)" data-footer="NHP Luminex Standard Curves (Cell Culture Supernates)" title="Cat. No. FCSTM21 - NHP Luminex Standard Curves (Cell Culture Supernates)" href="https://resources.rndsystems.com/images/datasheets/fcstm21_non-human-primate-xl-disc-premixed-luminex-perf-assay-kit-189202085348.png"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/fcstm21_non-human-primate-xl-disc-premixed-luminex-perf-assay-kit-189202085348.png" class="img-fluid" alt="NHP Luminex Standard Curves (Cell Culture Supernates)" title="NHP Luminex Standard Curves (Cell Culture Supernates)"> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">NHP Luminex Standard Curves (Cell Culture Supernates)</span> </p> </div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/fcstm21.pdf?v=20241123" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/nhp-xl-cytokine-luminex-performance-premixed-kit_fcstm21">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//resources.rndsystems.com/pdfs/msds/fcstm21.pdf?v=20241123">SDS</a> </div> </div> </div> <div class="ds_lightBlueBar">Product Specific Notices</div> <div class="animal_bluebar"> Luminex® is a registered trademark of Luminex Corporation. </div> </div> <div class="tab_content tab_3" data-tab="3" data-tabname="product-citations" id="citations"> <a id="citations_section"></a> <h2><span class="citations">Citations for NHP XL Cytokine Luminex® Performance Premixed Kit</h2> <div id="citation"> <a id="dsTabCitations"></a> <p> R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. </p> <p> <strong><span id="maxcitations">4</span> Citations: Showing <span id="showfrom">1</span> - <span id="showto">4</span> </strong> <br/><strong>Filter your results:</strong> </p> <div class="row"> <div class="col-sm-12"> <div class="citations_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper filter_select"> <select id="selSpecies" title="Species"> <option value="All">All Species</option> <option class="citation_filterRadio" value="species" data-filter="Macaca leonina"> Macaca leonina </option> <option class="citation_filterRadio" value="species" data-filter="Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)"> Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque) </option> <option class="citation_filterRadio" value="species" data-filter="Primate - Macaca mulatta (Rhesus Macaque)"> Primate - Macaca mulatta (Rhesus Macaque) </option> <option class="citation_filterRadio" value="species" data-filter="Rhesus Macaque"> Rhesus Macaque </option> </select> </div> <input type="hidden" id="selAntibody" value="All"> <div class="select_wrapper filter_select"> <select id="selSampleType" title="Sample Types"> <option value="All">All Sample Types</option> <option class="citation_filterRadio" value="sampletype" data-filter="Cell Culture Supernates"> Cell Culture Supernates </option> <option class="citation_filterRadio" value="sampletype" data-filter="Plasma"> Plasma </option> <option class="citation_filterRadio" value="sampletype" data-filter="Serum"> Serum </option> </select> </div> </div> </div> </div> <div class="row"> <ol id="citations" class="col-xs-12" data-expandall="false" data-max-citations="4" data-max-show="10"> <li class="top-lined" data-species="Primate - Macaca mulatta (Rhesus Macaque)" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/37793211"> Chronic alcohol consumption dysregulates innate immune response to SARS-CoV-2 in the lung </a> </span><br/> Authors: Lewis, SA;Cinco, IR;Doratt, BM;Blanton, MB;Hoagland, C;Newman, N;Davies, M;Grant, KA;Messaoudi, I;<br/> EBioMedicine<br/> Species: Primate - Macaca mulatta (Rhesus Macaque)<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)" data-application="" data-sampletype="Plasma"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/37285333"> CCX559 is a potent, orally-administered small molecule PD-L1 inhibitor that induces anti-tumor immunity </a> </span><br/> Authors: Sullivan, KMC;Vilalta, M;Ertl, LS;Wang, Y;Dunlap, C;Ebsworth, K;Zhao, BN;Li, S;Zeng, Y;Miao, Z;Fan, P;Mali, V;Lange, C;McMurtrie, D;Yang, J;Lui, R;Scamp, R;Chhina, V;Kumamoto, A;Yau, S;Dang, T;Easterday, A;Liu, S;Miao, S;Charo, I;Schall, TJ;Zhang, P;<br/> PloS one<br/> Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Rhesus Macaque" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/35918043"> Recall responses in the lung environment are impacted by age in a pilot study of Mycobacterium bovis-BCG vaccinated rhesus macaques </a> </span><br/> Authors: JM Scordo, TJ Piergallin, AM Olmo-Fontá, A Thomas, HP Raué, M Slifka, J Turner<br/> Experimental gerontology, 2022-07-30;167(0):111904.<br/> Species: Rhesus Macaque<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Macaca leonina" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/35508619"> Liver-directed lentiviral gene therapy corrects hemophilia A mice and achieves normal-range factor VIII activity in non-human primates </a> </span><br/> Authors: M Milani, C Canepari, T Liu, M Biffi, F Russo, T Plati, R Curto, S Patarroyo-, D Drager, I Visigalli, C Brombin, P Albertini, A Follenzi, E Ayuso, C Mueller, A Annoni, L Naldini, A Cantore<br/> Nature Communications, 2022-05-04;13(1):2454.<br/> Species: Macaca leonina<br/> Sample Types: Serum<br/> </li> </ol> </div> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <ol id="faq_list"> <li> <span class="faq_question"><p>Which primate species have been tested with the NHP XL Cytokine Premixed Kit?</p></span> <ul class="faq_answer"> <li><p>The kit has been tested and validated with Cynomolgus monkey and Rhesus macaque samples. The kit is broadly designated as reactive with non-human primate samples due to the expected high homology across primate species.</p></li> </ul> </li> </ol> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/luminex-assays"> View all Luminex Assay FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="fcstm21" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for NHP XL Cytokine Luminex® Performance Premixed Kit</h3> <div class="row"> <div class="col-xs-12"> <div class="datasheet_section"> <div class="row"> <div class="col-md-6"> <p> Average Rating: 4.7 <span class="review_stars_5"></span> (Based on 3 Reviews) </p> <div class="review_rating_progress"> <div class="row"> <div class="col-xs-3"> 5 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="66.666666666667" aria-valuemin="0" aria-valuemax="100" style="width:66.666666666667%"> </div> </div> </div> <div class="col-xs-3"> 66.67% </div> </div> <div class="row"> <div class="col-xs-3"> 4 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="33.333333333333" aria-valuemin="0" aria-valuemax="100" style="width:33.333333333333%"> </div> </div> </div> <div class="col-xs-3"> 33.33% </div> </div> <div class="row"> <div class="col-xs-3"> 3 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="0" aria-valuemin="0" aria-valuemax="100" style="width:0%"> </div> </div> </div> <div class="col-xs-3"> 0% </div> </div> <div class="row"> <div class="col-xs-3"> 2 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="0" aria-valuemin="0" aria-valuemax="100" style="width:0%"> </div> </div> </div> <div class="col-xs-3"> 0% </div> </div> <div class="row"> <div class="col-xs-3"> 1 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="0" aria-valuemin="0" aria-valuemax="100" style="width:0%"> </div> </div> </div> <div class="col-xs-3"> 0% </div> </div> </div> </div> <div class="col-md-6"> <div class="well"> <h4>Have you used NHP XL Cytokine Luminex® Performance Premixed Kit?</h4> <h5><strong>Submit a review and receive an Amazon gift card.</strong></h5> <div class="review_reward_txt"> <p>$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image</p> <p>$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image</p> </div> <a class="btn btn-quinary" href="/reviews/FCSTM21"> Submit a Review </a> </div> </div> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="reviews_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper"> <select class="review_filter_rating"> <option value="all">All Ratings</option> <option value="5">5</option> <option value="4">4</option> </select> </div> <div class="select_wrapper"> <select class="review_filter_application"> <option value="all">All Applications</option> </select> </div> </div> </div> </div> <br/> <div class="review_row" data-rating="4" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_4">NHP XL Cytokine Luminex® Performance Premixed Kit</div> <div class="review_date">By <span class="review_reviewer">Anonymous</span> on 06/15/2022 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> Blood mononuclear cells (PBMCs) </span> </div> </div> </div> <div class="col-xs-12 col-sm-2"> <a class="ds-image" data-toggle="lightbox" href="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_fcstm21-06_73431_0.jpg" data-title="NHP XL Cytokine Luminex® Performance Premixed Kit FCSTM21" data-gallery="Review-images"> <img loading="lazy" class="img-fluid review_image" alt="NHP XL Cytokine Luminex® Performance Premixed Kit FCSTM21" title="NHP XL Cytokine Luminex® Performance Premixed Kit FCSTM21" src="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_fcstm21-06_73431_0.jpg"/> </a> </div> </div> <hr/> </div> <div class="review_row" data-rating="5" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_5">NHP XL Cytokine Luminex Performance Premixed Kit</div> <div class="review_date">By <span class="review_reviewer">Lu Bai</span> on 05/06/2022 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> Non human primary </span> </div> </div> </div> </div> <hr/> </div> <div class="review_row" data-rating="5" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_5">NHP XL Cytokine Luminex Performance Premixed Kit</div> <div class="review_date">By <span class="review_reviewer">Anonymous</span> on 10/16/2020 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> Plasma </span> </div> </div> <div class="review_how_used"><p>This kit works well with Drop Array plates</p></div> </div> <div class="col-xs-12 col-sm-2"> <a class="ds-image" data-toggle="lightbox" href="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_fcstm21_57571_0.png" data-title="NHP XL Cytokine Luminex Performance Premixed Kit FCSTM21" 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src="//resources.rndsystems.com/images/Pathways/Thumbnails/il-9-signaling-pathways-effects.png"> </a> </li> <li class="pathway_wrapper"> <a class="pathway_link" href="https://www.rndsystems.com/pathways/inflammasome-activation-pathways"> <span class="pathway_title">Inflammasome Activation Pathways</span> <img class="pathway_image" loading="lazy" alt="Inflammasome Activation Pathways" src="//resources.rndsystems.com/images/Pathways/Thumbnails/inflammasome-activation-pathways.png"> </a> </li> <li class="pathway_wrapper"> <a class="pathway_link" href="https://www.rndsystems.com/pathways/innate-lymphoid-cells-differentiation"> <span class="pathway_title">Innate Lymphoid Cells Differentiation</span> <img class="pathway_image" loading="lazy" alt="Innate Lymphoid Cells Differentiation" src="//resources.rndsystems.com/images/Pathways/Thumbnails/innate-lymphoid-cells-differentiation.jpg"> </a> </li> <li class="pathway_wrapper"> <a class="pathway_link" href="https://www.rndsystems.com/pathways/jak-stat-signaling-pathway"> <span class="pathway_title">Jak/STAT Signaling Pathway</span> <img class="pathway_image" loading="lazy" alt="Jak/STAT Signaling Pathway" src="//resources.rndsystems.com/images/Pathways/Thumbnails/jak-stat-signaling-pathways.png"> </a> </li> <li class="pathway_wrapper"> <a class="pathway_link" href="https://www.rndsystems.com/pathways/mesenchymal-stem-cells-lineage-specific-markers"> <span class="pathway_title">Mesenchymal Stem Cells &amp; Lineage-specific Markers</span> <img class="pathway_image" loading="lazy" alt="Mesenchymal Stem Cells &amp;amp; Lineage-specific Markers" src="//resources.rndsystems.com/images/Pathways/Thumbnails/mesenchymal-stem-cells.png"> </a> </li> <li class="pathway_wrapper"> <a class="pathway_link" href="https://www.rndsystems.com/pathways/mtor-signaling"> <span class="pathway_title">mTOR Signaling</span> <img class="pathway_image" loading="lazy" alt="mTOR Signaling" 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