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Quantitative PCR

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class="navbar_item__jtENX"><a href="/solutions/gene-editing-toolkit" class="hover-border">Gene Editing Toolkit</a></div></div><div class="navbar_subNavbarCol__ES8j1"><div class="navbar_item__jtENX"><p class="p-xs color-xLight">Providers</p></div><div class="navbar_item__jtENX"><a href="/solutions/ngs-solutions-providers" class="hover-border">NGS Solution Providers</a></div></div></div></div></div></nav><div class="content"><main class="subPage_main___aCKT"><div class="subPage_heroContainer__C4Thz"><div class="container_container__7U8A5"><div class="subPage_hero__3ifPE"><div class="subPage_heroContent__6tnnS"><h1 class="h2">qPCR</h1><p class="p-lg">Replace spreadsheet formulas with an automated and reproducible solution to calculate transcript abundance</p><div class="subPage_buttonContainer___xiv_"><div><div class="requestDemoForm_inlineForm__WV3Xi"><input type="email" autoComplete="email" placeholder="Enter your work email…" value=""/><button type="button" class="bold button_md__WVu1z button_normal__dQOO7 button_btn__DjQft" rel="noopener"><span class="button_glow__U5paJ" style="left:0px;top:0px"></span><span class="button_btnContent__oxNmH">Free Trial<!-- --> <div class="button_arrow__2z32B"><svg viewBox="0 0 24 24" fill="none" xmlns="http://www.w3.org/2000/svg" class="icon"><g style="transform:rotate(0deg);transform-origin:center"><path d="M5 12H19" stroke="currentcolor" stroke-width="2" stroke-linecap="round" stroke-linejoin="round"></path><path d="M12 5L19 12L12 19" stroke="currentcolor" stroke-width="2" stroke-linecap="round" stroke-linejoin="round"></path></g></svg></div></span></button></div></div></div></div></div></div></div><div class="container_container__7U8A5 subPage_section__oSAPp"><hr class="subPage_spaceDivider__X0obF subPage_divider__bcdjq"/><div class="subPage_twoColSection__9IX_2"><div><p class="p-md">Overview</p></div><div><div class="subPage_textBlock__k_kL6"><h2 class="p-xl regular color-medium">Data infrastructure and tools to tell you how much mRNA is in your sample</h2></div><div class="subPage_imageSectionContainer__lQ_fV"><video preload="none" poster="/_next/static/media/qpcr-plots-layout-placeholder.ac93a5bb.png" placeholder="/_next/static/media/qpcr-plots-layout-placeholder.ac93a5bb.png" loop="" muted="" playsInline="" class="subPage_image__8VfBu"><source src="/_next/static/videos/qpcr-plots-layout-1b15f7441555ecac1fb6af6c109129f2.mp4" type="video/mp4"/></video></div></div></div></div><div class="container_container__7U8A5 subPage_sectionbottom__kUALZ"><hr class="subPage_spaceDivider__X0obF subPage_divider__bcdjq"/><div class="subPage_twoColSection__9IX_2"><div><p class="p-md">Benefits</p></div><div><ul class="list list-no-bullet subPage_benefitsList__NBlvS"><li class="p-s text-block"><div class="featureItem_container__CMU5e subPage_alignCenter__7jx3K" role="listitem"><div class="featureItem_iconContainer__SHHQN"><!--$!--><template data-dgst="DYNAMIC_SERVER_USAGE"></template><!--/$--></div><div class="featureItem_textContainer__0ZABB"><p class="p-lg">How do my raw Cqs vary between wells?</p></div></div></li><hr class="subPage_divider__bcdjq"/><li class="p-s text-block"><div class="featureItem_container__CMU5e subPage_alignCenter__7jx3K" role="listitem"><div class="featureItem_iconContainer__SHHQN"><!--$!--><template data-dgst="DYNAMIC_SERVER_USAGE"></template><!--/$--></div><div class="featureItem_textContainer__0ZABB"><p class="p-lg">What are the ∆∆Cqs for each condition?</p></div></div></li><hr class="subPage_divider__bcdjq"/><li class="p-s text-block"><div class="featureItem_container__CMU5e subPage_alignCenter__7jx3K" role="listitem"><div class="featureItem_iconContainer__SHHQN"><!--$!--><template data-dgst="DYNAMIC_SERVER_USAGE"></template><!--/$--></div><div class="featureItem_textContainer__0ZABB"><p class="p-lg">How does the fold change of my target gene change between conditions?</p></div></div></li></ul></div></div></div><div class="container_container__7U8A5 subPage_sectionbottom__kUALZ"><hr class="subPage_spaceDivider__X0obF subPage_divider__bcdjq"/><div class="subPage_twoColSection__9IX_2"><div><p class="p-s">Join leading biopharma companies who analyze their data on Latch</p></div><div><div class="subPage_logosRow__AUYXj"><img alt="Elsie Logo" loading="lazy" width="128" height="66.66666666666667" decoding="async" data-nimg="1" style="color:transparent" src="/_next/static/media/elsie.3656c15f.svg"/><img alt="Switch Logo" loading="lazy" width="118.66666666666667" height="66.66666666666667" decoding="async" data-nimg="1" style="color:transparent" src="/_next/static/media/switch-theraputics.85d0d4b8.svg"/><img alt="Orgonovo Logo" loading="lazy" width="175.33333333333334" height="66.66666666666667" decoding="async" data-nimg="1" style="color:transparent" src="/_next/static/media/organovo.a5ec1dfc.svg"/><img alt="Navega Logo" loading="lazy" width="126.66666666666667" height="66.66666666666667" decoding="async" data-nimg="1" style="color:transparent" src="/_next/static/media/navega-therapeutics.1ebc348f.svg"/><img alt="Dolororimics Logo" loading="lazy" width="205.33333333333334" height="66.66666666666667" decoding="async" data-nimg="1" style="color:transparent" src="/_next/static/media/doloromics.c79c8205.svg"/><img alt="Secarna Logo" loading="lazy" width="196" height="66.66666666666667" decoding="async" data-nimg="1" style="color:transparent" src="/_next/static/media/secarna.a7619027.svg"/></div></div></div></div><div class="container_container__7U8A5 subPage_benefitsCentered__P5ATt"><div class="featureItem_container__CMU5e subPage_alignCenter__7jx3K" role="listitem"><div class="featureItem_iconContainer__SHHQN"><!--$!--><template data-dgst="DYNAMIC_SERVER_USAGE"></template><!--/$--></div><div class="featureItem_textContainer__0ZABB"><p class="p-lg">Literally just stop using GraphPad Prism and Excel</p></div></div><div class="featureItem_container__CMU5e subPage_alignCenter__7jx3K" role="listitem"><div class="featureItem_iconContainer__SHHQN"><!--$!--><template data-dgst="DYNAMIC_SERVER_USAGE"></template><!--/$--></div><div class="featureItem_textContainer__0ZABB"><p class="p-lg">Reproducible, automated and auditable analysis</p></div></div></div><div class="subPage_diffSection__ejLdp"><div class="container_container__7U8A5 subPage_section__oSAPp"><div class="subPage_timeLineContainer__C1rwY"><div class="subPage_timeLine__a8rf4"></div><div class="subPage_body__irQyX subPage_timeLineHeader__ibWG2"><p class="p-lg">Analysis Lifecycle</p></div></div><div class="subPage_timeLineContainer__C1rwY"><div class="subPage_timeLine__a8rf4"><div class="subPage_number__XT_3b subPage_step2__d5S3E"><svg viewBox="0 0 24 24" fill="none" xmlns="http://www.w3.org/2000/svg" class="subPage_icon__hkEMr"><path d="M5.00028 16.0001C5.00027 15.4479 5.44798 15.0001 6.00027 15.0001H6.01027C6.56255 15.0001 7.01027 15.4478 7.01028 16.0001C7.01029 16.5524 6.56259 17.0001 6.0103 17.0001H6.0003C5.44802 17.0001 5.00029 16.5524 5.00028 16.0001Z" fill="currentcolor"></path><path d="M10.0003 15.0001C9.44798 15.0001 9.00027 15.4478 9.00028 16.0001C9.00029 16.5524 9.44802 17.0001 10.0003 17.0001H10.0103C10.5626 17 11.0103 16.5523 11.0103 16C11.0103 15.4478 10.5626 15 10.0103 15.0001H10.0003Z" fill="currentcolor"></path><path fill-rule="evenodd" clip-rule="evenodd" d="M16.7601 2.99994L7.24006 3.0001C6.68193 3.00041 6.13443 3.15641 5.6601 3.45055C5.18624 3.74441 4.80366 4.16451 4.55528 4.66369L4.55456 4.66515L1.11504 11.5345C1.04172 11.6736 1.00021 11.8321 1.00021 12.0002L1.00032 18.0002C1.00033 18.7959 1.31642 19.5589 1.87903 20.1215C2.44165 20.6841 3.20472 21.0002 4.00037 21.0002L20.0004 20.9999C20.796 20.9999 21.5591 20.6838 22.1217 20.1212C22.6843 19.5585 23.0003 18.7955 23.0003 17.9998L23.0002 11.9998C23.0002 11.9871 23 11.9744 22.9995 11.9617C22.9937 11.8077 22.9531 11.6626 22.8854 11.5341L19.4456 4.66489L19.445 4.66357C19.1966 4.16434 18.8139 3.7442 18.34 3.45033C17.8657 3.1562 17.3182 3.00023 16.7601 2.99994ZM20.3811 10.9999L17.6559 5.55766L17.6546 5.55492C17.5718 5.38832 17.4442 5.24811 17.286 5.15007C17.128 5.05206 16.9457 5.00007 16.7598 4.99994L7.24043 5.0001C7.05446 5.00024 6.8722 5.05224 6.71415 5.15025C6.55604 5.2483 6.42842 5.38851 6.34563 5.55512L5.45009 5.11014L6.34427 5.55785L3.61928 11.0002L20.3811 10.9999ZM3.00023 13.0002L3.00032 18.0002C3.00032 18.2654 3.10568 18.5197 3.29322 18.7073C3.48076 18.8948 3.73512 19.0002 4.00034 19.0002L20.0003 18.9999C20.2656 18.9999 20.5199 18.8945 20.7074 18.707C20.895 18.5194 21.0003 18.2651 21.0003 17.9999L21.0002 12.9999L3.00023 13.0002Z" fill="currentcolor"></path></svg></div><div class="subPage_line__zNPwu"></div></div><div class="subPage_body__irQyX"><div class="subPage_textBlock__k_kL6 text-block"><h2 class="h5 luminescent-text">Stream to the Cloud from qPCR Machines</h2><p class="p-md">Scalable, cloud-native storage for machine outputs. A central location to organize teams. Graphical interface for scientists + CLI for programmers</p></div><div class="subPage_imageRow____XAk"><video preload="none" poster="/_next/static/media/qpcr-gdrive-import-placeholder.4b4319b4.png" placeholder="/_next/static/media/qpcr-gdrive-import-placeholder.4b4319b4.png" loop="" muted="" playsInline="" class="subPage_image__8VfBu"><source src="/_next/static/videos/qpcr-gdrive-import-2797a9475dcf1c3d428fdf74025e5103.mp4"/></video></div><div class="subPage_textBlock__k_kL6 text-block"><p class="p-md">Integrate directly with Google Drive. Drag files from the computer attached to your qPCR machine to a shared company Google account.</p></div></div></div><div class="subPage_timeLineContainer__C1rwY"><div class="subPage_timeLine__a8rf4"><div class="subPage_number__XT_3b subPage_step2__d5S3E"><svg viewBox="0 0 24 24" fill="none" xmlns="http://www.w3.org/2000/svg" class="subPage_icon__hkEMr"><path fill-rule="evenodd" clip-rule="evenodd" d="M15.7253 5.03849C16.2563 4.88677 16.8098 5.19426 16.9615 5.72529L20.9615 19.7253C21.1133 20.2563 20.8058 20.8098 20.2747 20.9615C19.7437 21.1133 19.1902 20.8058 19.0385 20.2747L15.0385 6.27473C14.8868 5.7437 15.1943 5.19021 15.7253 5.03849Z" fill="currentcolor"></path><path fill-rule="evenodd" clip-rule="evenodd" d="M12 5C12.5523 5 13 5.44772 13 6V20C13 20.5523 12.5523 21 12 21C11.4477 21 11 20.5523 11 20V6C11 5.44772 11.4477 5 12 5Z" fill="currentcolor"></path><path fill-rule="evenodd" clip-rule="evenodd" d="M8 7C8.55228 7 9 7.44772 9 8V20C9 20.5523 8.55228 21 8 21C7.44772 21 7 20.5523 7 20V8C7 7.44772 7.44772 7 8 7Z" fill="currentcolor"></path><path fill-rule="evenodd" clip-rule="evenodd" d="M4 3C4.55228 3 5 3.44772 5 4V20C5 20.5523 4.55228 21 4 21C3.44772 21 3 20.5523 3 20V4C3 3.44772 3.44772 3 4 3Z" fill="currentcolor"></path></svg></div><div class="subPage_line__zNPwu"></div></div><div class="subPage_body__irQyX"><div class="subPage_textBlock__k_kL6 text-block"><h2 class="h5 luminescent-text">Associate qPCR runs with experimental metadata</h2><p class="p-s">Create structured schemas to capture experimental context and associate with raw machine files + results</p></div><div class="subPage_imageRow____XAk"><img alt="" loading="lazy" width="1110" height="363" decoding="async" data-nimg="1" class="subPage_transparentImage__dKPV3 subPage_image__8VfBu" style="color:transparent" srcSet="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fqpc-data-registry.68b6d42d.png&amp;w=1200&amp;q=75 1x, /_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fqpc-data-registry.68b6d42d.png&amp;w=3840&amp;q=75 2x" src="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fqpc-data-registry.68b6d42d.png&amp;w=3840&amp;q=75"/></div><div class="subPage_fullWidthBento__OUUko subPage_fakeBentoBox__w0lIc"><p class="p-md color-medium">Use provided templates to fill out well plate metadata in software that scientists already trust - Excel + Sheets.</p><img alt="List of data within Latch" loading="lazy" width="844" height="730" decoding="async" data-nimg="1" class="subPage_image__8VfBu page_imageDownloadSpreadsheet__5G0fw" style="color:transparent" srcSet="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fqpcr-template-download.8f6675e2.png&amp;w=1080&amp;q=75 1x, /_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fqpcr-template-download.8f6675e2.png&amp;w=1920&amp;q=75 2x" src="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fqpcr-template-download.8f6675e2.png&amp;w=1920&amp;q=75"/><img alt="List of data within Latch" loading="lazy" width="844" height="730" decoding="async" data-nimg="1" class="subPage_image__8VfBu page_imageSpreadsheet__oHO_g" style="color:transparent" srcSet="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fqpcr-template.90636232.png&amp;w=1080&amp;q=75 1x, /_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fqpcr-template.90636232.png&amp;w=1920&amp;q=75 2x" src="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fqpcr-template.90636232.png&amp;w=1920&amp;q=75"/></div></div></div><div class="subPage_timeLineContainer__C1rwY"><div class="subPage_timeLine__a8rf4"><div class="subPage_number__XT_3b subPage_step2__d5S3E"><svg viewBox="0 0 24 24" fill="none" xmlns="http://www.w3.org/2000/svg" class="subPage_icon__hkEMr"><path fill-rule="evenodd" clip-rule="evenodd" d="M3 2C3.55228 2 4 2.44772 4 3V20H21C21.5523 20 22 20.4477 22 21C22 21.5523 21.5523 22 21 22H3C2.44772 22 2 21.5523 2 21V3C2 2.44772 2.44772 2 3 2ZM6 11C6 9.89543 6.89543 9 8 9H10C11.1046 9 12 9.89543 12 11V16C12 17.1046 11.1046 18 10 18H8C6.89543 18 6 17.1046 6 16V11ZM10 11H8V16H10V11ZM16 4C14.8954 4 14 4.89543 14 6V16C14 17.1046 14.8954 18 16 18H18C19.1046 18 20 17.1046 20 16V6C20 4.89543 19.1046 4 18 4H16ZM16 6H18V16H16V6Z" fill="currentcolor"></path></svg></div><div class="subPage_line__zNPwu"></div></div><div class="subPage_body__irQyX"><div class="subPage_textBlock__k_kL6 text-block"><h2 class="h5 luminescent-text">Configure experimental design and visualize Cqs</h2><p class="p-s">Experimental designs are very individual and complex. Select housekeeping genes, condition columns, control values. Bring in additional variables like dose and drug name</p></div><div class="subPage_fakeBentoGrid__m9H1u"><div class="subPage_fakeBentoBox__w0lIc"><p class="p-md color-medium">Setup the experiment.</p><img alt="List of data within Latch" loading="lazy" width="844" height="730" decoding="async" data-nimg="1" class="subPage_image__8VfBu" style="color:transparent;position:absolute;top:100px;left:0;max-width:80%;border-top-right-radius:var(--bento-image-radius)" srcSet="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fsetup-experiment.39d20b08.png&amp;w=1080&amp;q=75 1x, /_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fsetup-experiment.39d20b08.png&amp;w=1920&amp;q=75 2x" src="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fsetup-experiment.39d20b08.png&amp;w=1920&amp;q=75"/></div><div class="subPage_fakeBentoBox__w0lIc"><p class="p-md color-medium">And preview the study design.</p><img alt="List of data within Latch" loading="lazy" width="844" height="730" decoding="async" data-nimg="1" class="subPage_image__8VfBu" style="color:transparent;position:absolute;top:100px;left:0;max-width:80%;border-top-right-radius:var(--bento-image-radius)" srcSet="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fpreview-study-design.be972335.png&amp;w=1080&amp;q=75 1x, /_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fpreview-study-design.be972335.png&amp;w=1920&amp;q=75 2x" src="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fpreview-study-design.be972335.png&amp;w=1920&amp;q=75"/></div></div><div class="subPage_textBlock__k_kL6 text-block"><p class="p-s">Generate publication (or slide deck) quality visualizations with graphical control over eg. plot type, formatting, color scheme, labels.</p></div><div class="subPage_imageRow____XAk"><img alt="" loading="lazy" width="1164" height="460" decoding="async" data-nimg="1" class="subPage_image__8VfBu" style="color:transparent" srcSet="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fchart-cq-by-well.dc5f55c3.png&amp;w=1200&amp;q=75 1x, /_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fchart-cq-by-well.dc5f55c3.png&amp;w=3840&amp;q=75 2x" src="/_next/image?url=%2F_next%2Fstatic%2Fmedia%2Fchart-cq-by-well.dc5f55c3.png&amp;w=3840&amp;q=75"/></div></div></div><div class="subPage_timeLineContainer__C1rwY"><div class="subPage_timeLine__a8rf4"><div class="subPage_number__XT_3b subPage_step2__d5S3E"><svg viewBox="0 0 24 24" fill="none" xmlns="http://www.w3.org/2000/svg" class="subPage_icon__hkEMr"><path fill-rule="evenodd" clip-rule="evenodd" d="M3 2C3.55228 2 4 2.44772 4 3V20H21C21.5523 20 22 20.4477 22 21C22 21.5523 21.5523 22 21 22H3C2.44772 22 2 21.5523 2 21V3C2 2.44772 2.44772 2 3 2ZM6 11C6 9.89543 6.89543 9 8 9H10C11.1046 9 12 9.89543 12 11V16C12 17.1046 11.1046 18 10 18H8C6.89543 18 6 17.1046 6 16V11ZM10 11H8V16H10V11ZM16 4C14.8954 4 14 4.89543 14 6V16C14 17.1046 14.8954 18 16 18H18C19.1046 18 20 17.1046 20 16V6C20 4.89543 19.1046 4 18 4H16ZM16 6H18V16H16V6Z" fill="currentcolor"></path></svg></div><div class="subPage_line__zNPwu"></div></div><div class="subPage_body__irQyX"><div class="subPage_textBlock__k_kL6 text-block"><h2 class="h5 luminescent-text">Calculate ∆∆Cqs (without spreadsheets)</h2><p class="p-s">Calculate ∆∆Cqs with a button click, without spreadsheet formulas. Anyone on the team can access and modify underlying Python code.</p></div><div class="subPage_imageRow____XAk"><video preload="none" poster="/_next/static/media/qpcr-calculate-delta-delta-cq-placeholder.f2658efe.png" placeholder="/_next/static/media/qpcr-calculate-delta-delta-cq-placeholder.f2658efe.png" loop="" muted="" playsInline="" class="subPage_image__8VfBu"><source src="/_next/static/videos/qpcr-calculate-delta-delta-cq-a2ac986fb372184ad567d5815b0dc265.mp4"/></video></div></div></div><div class="subPage_timeLineContainer__C1rwY"><div class="subPage_timeLine__a8rf4"><div class="subPage_number__XT_3b subPage_step2__d5S3E"><svg viewBox="0 0 24 24" fill="none" xmlns="http://www.w3.org/2000/svg" class="subPage_icon__hkEMr"><path fill-rule="evenodd" clip-rule="evenodd" d="M3 2C3.55228 2 4 2.44772 4 3V20H21C21.5523 20 22 20.4477 22 21C22 21.5523 21.5523 22 21 22H3C2.44772 22 2 21.5523 2 21V3C2 2.44772 2.44772 2 3 2ZM6 11C6 9.89543 6.89543 9 8 9H10C11.1046 9 12 9.89543 12 11V16C12 17.1046 11.1046 18 10 18H8C6.89543 18 6 17.1046 6 16V11ZM10 11H8V16H10V11ZM16 4C14.8954 4 14 4.89543 14 6V16C14 17.1046 14.8954 18 16 18H18C19.1046 18 20 17.1046 20 16V6C20 4.89543 19.1046 4 18 4H16ZM16 6H18V16H16V6Z" fill="currentcolor"></path></svg></div><div class="subPage_line__zNPwu subPage_short__Bwrc7"></div></div><div class="subPage_body__irQyX"><div class="subPage_textBlock__k_kL6 text-block"><h2 class="h5 luminescent-text">Remove outliers and visualize target gene fold changes</h2></div><div class="subPage_paddingTop__ifHaU"><div class="subPage_imageRow____XAk"><video preload="none" poster="/_next/static/media/qpcr-calculate-delta-delta-cq-placeholder.f2658efe.png" placeholder="/_next/static/media/qpcr-calculate-delta-delta-cq-placeholder.f2658efe.png" loop="" muted="" playsInline="" class="subPage_image__8VfBu"><source src="/_next/static/videos/qpcr-outliers-ed2049da2f2b1864381c8365886d2b23.mp4"/></video></div></div></div></div></div></div><div class="container_container__7U8A5 subPage_sectionbottom__kUALZ"><div class="subPage_cta__XvjSN"><h3 class="luminescent-text center">Scientists wasting time in Excel and GraphPad Prism?<!-- --> </h3><p class="p-md center">Eliminate manual clicking and user error with an automated plotting solution for your lab.</p></div><div class="requestTrialBanner_container__LUVGK"><p class="h4">7-Day Free Trial</p><p class="p-md">Access the leading data platform for biology.</p><ul class="list-no-bullet list p-s requestTrialBanner_list__LHfd9"><li><svg xmlns="http://www.w3.org/2000/svg" width="24" height="24" viewBox="0 0 24 24" fill="none" stroke="currentColor" stroke-width="2" stroke-linecap="round" stroke-linejoin="round" class="lucide lucide-circle-check"><circle cx="12" cy="12" r="10"></circle><path d="m9 12 2 2 4-4"></path></svg> A scoping call to map out your scientific workflows</li><li><svg xmlns="http://www.w3.org/2000/svg" width="24" height="24" viewBox="0 0 24 24" fill="none" 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