CINXE.COM

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No. FAB94972N - Human Adenosine A2aR/A2bR Alexa&nbsp;Fluor®&nbsp;700-conjugated Antibody" href="https://resources.rndsystems.com/categoryimages/rndsystems_antibodies.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/categoryimages/rndsystems_antibodies.jpg" class="img-fluid" alt="R&D Systems Antibodies" title="R&D Systems Antibodies"> </a> <div class="search_image_other_count"> <span class="search_more_images">1 Image</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/adenosine-a2ar-a2br?category=Primary Antibodies" class="btn btn-secondary">All Adenosine A2aR/A2bR Antibodies</a> </div> </div> <div class="tabbed_content" id="rnd_antibody"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_preparation_storage" id="product-anchor-ds_preparation_storage">Preparation and Storage</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_calculator" id="product-anchor-ds_calculator">Reconstitution Calculator</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_related_research_areas" id="product-anchor-ds_related_research_areas">Related Research Areas</a> </div> <h2 id="ds_summary">Human Adenosine A2aR/A2bR Alexa Fluor® 700-conjugated Antibody Summary</h2> <div class="datasheet_section"> <div class="ds_table summary_table"> <div class="ds_table_body">  <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Species Reactivity</div> <div class="ds_table_cell summary_table_data"> Human </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Specificity</div> <div class="ds_table_cell summary_table_data"> Detects human Adenosine A2aR and human Adenosine A2bR in flow cytometry. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Source</div> <div class="ds_table_cell summary_table_data"> Monoclonal Mouse IgG<sub>2B</sub> Clone # 599743 </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Purification</div> <div class="ds_table_cell summary_table_data"> Protein A or G purified from hybridoma culture supernatant </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Immunogen</div> <div class="ds_table_cell summary_table_data"> NS0 mouse myeloma cell line transfected with human Adenosine A2aR<br />Met1-Ser412<br />Accession # P29274 </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Formulation</div> <div class="ds_table_cell summary_table_data"> Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Label</div> <div class="ds_table_cell summary_table_data"> Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm) </div> </div> </div> </div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="FAB94972N"></div> <div id="ds_applications" class="datasheet_section"> <h2>Applications</h2> <div class="ds_table app_table"> <div class="ds_table_head"> <div class="ds_table_head_row"> <div class="ds_table_head_cell app_table_blank"></div> <div class="ds_table_head_cell app_table_rec_conc">Recommended Concentration</div> <div class="ds_table_head_cell app_table_sample">Sample</div> </div> </div> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell app_table_name">Flow Cytometry</div> <div class="ds_table_cell app_table_dilution">0.25-1 µg/10<sup>6</sup> cells</div> <div class="ds_table_cell app_table_sample">HEK293 Human Cell Line Transfected with Human Adenosine A2aR or Adenosine A2bR and eGFP</div> </div> </div> </div> <p>Please Note: Optimal dilutions should be determined by each laboratory for each application. <a href="/resources/protocols-troubleshooting-guides">General Protocols</a> are available in the Technical Information section on our website. </p> </div> <div id="ds_calculator" class="datasheet_section"> <div id="btn_calculator_wrapper"><div class="datasheet_calculator_toggle btn btn-secondary" id="btn_reconstitution">Reconstitution Calculator</div></div><div id="datasheet_calculators"><div id="btn_reconstitution_calc" class="datasheet_calculator"><h2>Reconstitution Calculator</h2><div id="reconstitution_calculator" class="calculator"> <form id="recCalc"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume (to add to vial)</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </span> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass (in vial)</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_mass_unit" class="calculator_input calculator_select"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </span> </div> <span class="calculator_divide calculator_operator">÷</span> <div class="calc_section"> <label for="reconstitution_calculator_concentration" class="calculator_label">Desired Reconstitution Concentration</label> <input id="reconstitution_calculator_concentration" name="reconstitution_calculator_concentration" type="text" maxlength="16" placeholder="conc." class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_concentration_unit" class="calculator_input calculator_select"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">ug/ml</option> <option value="ng/ml">ng/ml</option> </select> </span> </div> <div id="reconstitution_foot" class="calc_section"> <input type="submit" value="Calculate" id="button_reconstitution_calculator_calculate" class="calculator_button btn btn-secondary"/> </div> </form> </div> </div></div> </div> <div id="ds_preparation_storage" class="datasheet_section"> <h2>Preparation and Storage</h2> <div class="ds_table prep_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Shipping</div> <div class="ds_table_cell prep_table_data">The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Stability & Storage</div> <div class="ds_table_cell prep_table_data"><b>Protect from light. </b><b>Do not freeze.</b><UL><LI>12 months from date of receipt, 2 to 8 °C as supplied.</LI></UL> </div> </div> </div> </div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: Adenosine A2aR/A2bR</h2> <p><div>Adenosine is a ubiquitous endogenous molecule that affects almost all aspects of cellular physiology. The four Adenosine G protein-coupled receptors (GPCR) A<sub>1</sub>, A<sub>2A</sub>, A<sub>2B</sub>, and A<sub>3</sub> have been described as key metabolic and immune-checkpoint regulators implicated in the tumor escape from the host immune system becoming both, markers of pathologies, and useful targets for novel drugs. Adenosine receptors A<sub>2A</sub> and A<sub>2B</sub> (also known as ADORA2A and ADORA2B, respectively) have been also shown to play an important cardio-protective role.<br></div></p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Long Name</div> <div class="ds_table_cell background_table_data">Adenosine A2A Receptor/Adenosine A2B Receptor</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names</div> <div class="ds_table_cell background_table_data">Adenosine A2aR/A2bR</div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/fab94972n.pdf?v=20241105" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/human-adenosine-a2ar-a2br-alexa-fluor-700-conjugated-antibody-599743_fab94972n">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//aero.bio-techne.com/sds/pdf/fab94972n?v=20241105">SDS</a> </div> </div> </div> <div id="ds_animal_free" class="datasheet_section"> <h2>Product Specific Notices</h2> <br>This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354. </div> <div id="ds_related_research_areas" class="datasheet_section"> <h2>Related Research Areas</h2> <ul class="ds_details"> <li> <a href="/research-area/adenosine-receptors">Adenosine Receptors</a> </li> <li> <a href="/research-area/gpcrs-in-blood-pressure-regulation">GPCRs in Blood Pressure Regulation</a> </li> <li> <a href="/research-area/microglia-activation">Microglia Activation</a> </li> <li> <a href="/research-area/neurotransmitter-g-protein--coupled-receptors">Neurotransmitter G Protein-Coupled Receptors</a> </li> <li> <a href="/research-area/platelet-gpcrs">Platelet GPCRs</a> </li> </ul> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/primary-antibodies"> View all Antibody FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="fab94972n" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for Human Adenosine A2aR/A2bR Alexa Fluor® 700-conjugated Antibody</h3> <div class="row"> <div class="col-xs-12"> <div> <div class="row"> <div class="col-md-6"> <p>There are currently no reviews for this product. 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