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SDS-PAGE and Sephacryl S-200 gel filtration were used to provide information about the molecular mass of the protein purified from tartary buckwheat. The protein was composed of 215 amino acid residues and showed strong IgE binding activity in an ELISA test to the sera colleted from two patients allergic to buckwheat. These results suggested that the purified 24 kDa protein from tartary buckwheat seeds was an important functional protein and was relatively specific for buckwheatallergic patients. It should be a very useful tool in the diagnosis of buckwheat allergy in the future.","publication_date":"2004,,","publication_name":"Bioscience, Biotechnology, and Biochemistry","grobid_abstract_attachment_id":"42757084"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Purification and Characterization of a 24 kDa Protein from Tartary Buckwheat Seeds","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [35205987]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "full_page_mobile_sutd_modal"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div 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data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><div class="ds-top-related-works--grid-container"><div class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="54968386" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/54968386/Cloning_Expression_and_Identification_of_Immunological_Activity_of_an_Allergenic_Protein_in_Tartary_Buckwheat">Cloning, Expression, and Identification of Immunological Activity of an Allergenic Protein in Tartary Buckwheat</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="36143838" 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href="https://www.academia.edu/54968386/Cloning_Expression_and_Identification_of_Immunological_Activity_of_an_Allergenic_Protein_in_Tartary_Buckwheat"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="56461278" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/56461278/Buckwheat_Fagopyrum_Esculentum_Allergenic_Seed_Proteins_are_Restricted_to_the_Embryo_and_not_Found_in_the_Endosperm">Buckwheat (Fagopyrum Esculentum) Allergenic Seed Proteins are Restricted to the Embryo and not Found in the Endosperm</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="35205987" href="https://independent.academia.edu/IvanKreft">Ivan Kreft</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2007</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Buckwheat (Fagopyrum Esculentum) Allergenic Seed Proteins are Restricted to the Embryo and not Found in the Endosperm","attachmentId":71837341,"attachmentType":"pdf","work_url":"https://www.academia.edu/56461278/Buckwheat_Fagopyrum_Esculentum_Allergenic_Seed_Proteins_are_Restricted_to_the_Embryo_and_not_Found_in_the_Endosperm","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/56461278/Buckwheat_Fagopyrum_Esculentum_Allergenic_Seed_Proteins_are_Restricted_to_the_Embryo_and_not_Found_in_the_Endosperm"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="61015073" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/61015073/Immunochemical_analysis_of_buckwheat_proteins_prolamins_and_their_allergenic_character">Immunochemical analysis of buckwheat proteins, prolamins and their allergenic character</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="37152638" href="https://independent.academia.edu/PeterBiacs">Peter Biacs</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Acta Alimentaria, 1999</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Immunochemical analysis of buckwheat proteins, prolamins and their allergenic character","attachmentId":74206370,"attachmentType":"pdf","work_url":"https://www.academia.edu/61015073/Immunochemical_analysis_of_buckwheat_proteins_prolamins_and_their_allergenic_character","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/61015073/Immunochemical_analysis_of_buckwheat_proteins_prolamins_and_their_allergenic_character"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="103653723" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/103653723/A_Review_on_Epidemiological_and_Clinical_Studies_on_Buckwheat_Allergy">A Review on Epidemiological and Clinical Studies on Buckwheat Allergy</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="36143838" href="https://independent.academia.edu/GunillaWieslander">Gunilla Wieslander</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Plants, 2021</p><p class="ds-related-work--abstract ds2-5-body-sm">Background: Cultivated buckwheat include two species originating from China: common buckwheat (Fagopyrum esculentum) and tartary buckwheat (Fagopyrum tartaricum). Buckwheat can cause IgE-mediated allergy, including severe allergic reactions and anaphylaxis. Exposure can occure when eating buckwheat food (food allergen), when producing or handling buckwheat food (occupational exposure) or when sleeping on buckwheat husk pillows (houeshold environmental exposure). Methods: A search on buckwheat allergy in the medical datbase PubMed from 1970–2020. Result: A number of allergenic proteins have been identified in common buckwheat (e.g., Fag e 1, Fag e 2 and Fag e 3) and in tartary buckwheat (e.g., Fag t 1, Fag t 2, Fag t 3). Clinically relevant cross-reactivity has been described between buckwheat and peanut, latex, coconut, quinoa, and poppy seed. The prevalence of buckwheat allergy in the population can be estimated as 0.1–0.4% in Japan, Korea and buckwheat consuming areas of China. Am...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"A Review on Epidemiological and Clinical Studies on Buckwheat Allergy","attachmentId":103604642,"attachmentType":"pdf","work_url":"https://www.academia.edu/103653723/A_Review_on_Epidemiological_and_Clinical_Studies_on_Buckwheat_Allergy","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/103653723/A_Review_on_Epidemiological_and_Clinical_Studies_on_Buckwheat_Allergy"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="2391106" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/2391106/Clinical_manifestations_co_sensitizations_and_immunoblotting_profiles_of_buckwheat_allergic_patients">Clinical manifestations, co-sensitizations, and immunoblotting profiles of buckwheat-allergic patients</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="3094791" href="https://unito.academia.edu/EnricoHeffler">Enrico Heffler</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Allergy, 2011</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Clinical manifestations, co-sensitizations, and immunoblotting profiles of buckwheat-allergic patients","attachmentId":50647068,"attachmentType":"pdf","work_url":"https://www.academia.edu/2391106/Clinical_manifestations_co_sensitizations_and_immunoblotting_profiles_of_buckwheat_allergic_patients","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/2391106/Clinical_manifestations_co_sensitizations_and_immunoblotting_profiles_of_buckwheat_allergic_patients"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="56949002" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/56949002/Wild_Buckwheat_Is_Unlikely_to_Pose_a_Risk_to_Buckwheat_Allergic_Individuals">Wild Buckwheat Is Unlikely to Pose a Risk to Buckwheat-Allergic Individuals</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="31803907" href="https://un-lincoln.academia.edu/RichardGoodman">Richard Goodman</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Food Science, 2011</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Wild Buckwheat Is Unlikely to Pose a Risk to Buckwheat-Allergic Individuals","attachmentId":72085440,"attachmentType":"pdf","work_url":"https://www.academia.edu/56949002/Wild_Buckwheat_Is_Unlikely_to_Pose_a_Risk_to_Buckwheat_Allergic_Individuals","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/56949002/Wild_Buckwheat_Is_Unlikely_to_Pose_a_Risk_to_Buckwheat_Allergic_Individuals"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="2391145" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/2391145/ORIGINAL_ARTICLE_Clinical_manifestations_co_sensitizations_and_immunoblotting_profiles_of_buckwheat_allergic_patients_Buckwheat_allergy_in_a_series_of_24_patients">ORIGINAL ARTICLE: Clinical manifestations, co-sensitizations, and immunoblotting profiles of buckwheat-allergic patients: Buckwheat allergy in a series of 24 patients</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="3094791" href="https://unito.academia.edu/EnricoHeffler">Enrico Heffler</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Allergy, 2010</p><p class="ds-related-work--abstract ds2-5-body-sm">To cite this article: Heffler E, Nebiolo F, Asero R, Guida G, Badiu I, Pizzimenti S, Marchese C, Amato S, Mistrello G, Canaletti F, Rolla G. Clinical manifestations, co-sensitizations, and immunoblotting profiles of buckwheat-allergic patients. Allergy 2011; 66: 264–270.To cite this article: Heffler E, Nebiolo F, Asero R, Guida G, Badiu I, Pizzimenti S, Marchese C, Amato S, Mistrello G, Canaletti F, Rolla G. Clinical manifestations, co-sensitizations, and immunoblotting profiles of buckwheat-allergic patients. Allergy 2011; 66: 264–270.AbstractBackground: Buckwheat allergy is a rare food allergy in Europe and North America, whereas it is often described and studied in Asia. The aim of this study was to describe a series of patients with proven buckwheat allergy evaluated in an Italian allergy clinic. Co-sensitization to other food and inhalant allergens and immunoblotting profiles of buckwheat-allergic patients were studied.Methods: Patients with suspected buckwheat allergy who attended the allergy clinic between January 1, 2006, and September 30, 2008, were evaluated. All patients underwent skin prick tests for a standard panel of inhalant and food allergens, prick-by-prick with buckwheat flour, buckwheat-specific IgE determinations, and double-blind placebo-controlled food challenge (DBPCFC) with buckwheat flour. Immunoblotting with buckwheat flour extract was performed on sera from buckwheat-allergic patients.Results: Among 72 patients with suspected buckwheat allergy, 30 (41.7%) were sensitized to buckwheat and 24 had a positive DBPCFC. The mean buckwheat IgE level was 6.23 kUA/l (range, 0.16 to >100 kUA/l). Several IgE-binding proteins were identified and grouped into three patterns: a 16-kDa band in patients with predominantly gastrointestinal symptoms with grass and wheat flour co-sensitization, a 25-kDa band in patients with predominantly cutaneous symptoms and a low frequency of co-sensitization, and a 40-kDa band in patients with anaphylaxis and a low frequency of co-sensitization.Conclusions: Buckwheat allergy is an emerging food allergy in Italy. We identified three distinct patterns of clinical and laboratory characteristics, suggesting that specific allergens could be more frequently associated with clinical manifestations of different severity.Background: Buckwheat allergy is a rare food allergy in Europe and North America, whereas it is often described and studied in Asia. The aim of this study was to describe a series of patients with proven buckwheat allergy evaluated in an Italian allergy clinic. Co-sensitization to other food and inhalant allergens and immunoblotting profiles of buckwheat-allergic patients were studied.Methods: Patients with suspected buckwheat allergy who attended the allergy clinic between January 1, 2006, and September 30, 2008, were evaluated. All patients underwent skin prick tests for a standard panel of inhalant and food allergens, prick-by-prick with buckwheat flour, buckwheat-specific IgE determinations, and double-blind placebo-controlled food challenge (DBPCFC) with buckwheat flour. Immunoblotting with buckwheat flour extract was performed on sera from buckwheat-allergic patients.Results: Among 72 patients with suspected buckwheat allergy, 30 (41.7%) were sensitized to buckwheat and 24 had a positive DBPCFC. The mean buckwheat IgE level was 6.23 kUA/l (range, 0.16 to >100 kUA/l). Several IgE-binding proteins were identified and grouped into three patterns: a 16-kDa band in patients with predominantly gastrointestinal symptoms with grass and wheat flour co-sensitization, a 25-kDa band in patients with predominantly cutaneous symptoms and a low frequency of co-sensitization, and a 40-kDa band in patients with anaphylaxis and a low frequency of co-sensitization.Conclusions: Buckwheat allergy is an emerging food allergy in Italy. We identified three distinct patterns of clinical and laboratory characteristics, suggesting that specific allergens could be more frequently associated with clinical manifestations of different severity.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"ORIGINAL ARTICLE: Clinical manifestations, co-sensitizations, and immunoblotting profiles of buckwheat-allergic patients: Buckwheat allergy in a series of 24 patients","attachmentId":50647052,"attachmentType":"pdf","work_url":"https://www.academia.edu/2391145/ORIGINAL_ARTICLE_Clinical_manifestations_co_sensitizations_and_immunoblotting_profiles_of_buckwheat_allergic_patients_Buckwheat_allergy_in_a_series_of_24_patients","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/2391145/ORIGINAL_ARTICLE_Clinical_manifestations_co_sensitizations_and_immunoblotting_profiles_of_buckwheat_allergic_patients_Buckwheat_allergy_in_a_series_of_24_patients"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="10226093" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/10226093/Improvement_of_surface_functionalities_including_allergenicity_attenuation_of_whole_buckwheat_protein_fraction_by_maillard_type_glycation_with_dextran">Improvement of surface functionalities, including allergenicity attenuation, of whole buckwheat protein fraction by maillard-type glycation with dextran. </a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="6375356" href="https://independent.academia.edu/%E7%94%B0%E6%BE%A4%E8%8C%82">田澤茂</a></div><p class="ds-related-work--abstract ds2-5-body-sm">The purpose of the current study was to determine the effects of the introduction of polysaccharide chains onto the molecular surface of buckwheat proteins on buckwheat protein surface functionality. The whole buckwheat protein fraction (WBP) was prepared using 50 mM phosphate buffer (pH 7.5) containing 0.5 M NaCl and covalently linked with 6 kDa, 17.5 kDa, 40 kDa, 70 kDa, or 200 kDa dextran by Maillard-type glycation through controlled dry-heating at 60°C and 79% relative humidity for two weeks. Conjugation with 40 kDa dextran improved the water solubility and emulsifying properties of WBP without causing a serious loss of available lysine; 84.9% of the free amino groups were conserved. In addition, we found that the introduction of dextran chains onto the molecular surfaces of WBP attenuated the antigenicity of WBP.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Improvement of surface functionalities, including allergenicity attenuation, of whole buckwheat protein fraction by maillard-type glycation with dextran. ","attachmentId":36320403,"attachmentType":"pdf","work_url":"https://www.academia.edu/10226093/Improvement_of_surface_functionalities_including_allergenicity_attenuation_of_whole_buckwheat_protein_fraction_by_maillard_type_glycation_with_dextran","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/10226093/Improvement_of_surface_functionalities_including_allergenicity_attenuation_of_whole_buckwheat_protein_fraction_by_maillard_type_glycation_with_dextran"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="16080724" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/16080724/Buckwheat_Fagopyrum_esculentum_Moench_low_molecular_weight_seed_proteins_are_restricted_to_the_embryo_and_are_not_detectable_in_the_endosperm">Buckwheat (Fagopyrum esculentum Moench) low molecular weight seed proteins are restricted to the embryo and are not detectable in the endosperm</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="35205987" href="https://independent.academia.edu/IvanKreft">Ivan Kreft</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Plant Physiology and Biochemistry, 2005</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Buckwheat (Fagopyrum esculentum Moench) low molecular weight seed proteins are restricted to the embryo and are not detectable in the endosperm","attachmentId":42756383,"attachmentType":"pdf","work_url":"https://www.academia.edu/16080724/Buckwheat_Fagopyrum_esculentum_Moench_low_molecular_weight_seed_proteins_are_restricted_to_the_embryo_and_are_not_detectable_in_the_endosperm","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/16080724/Buckwheat_Fagopyrum_esculentum_Moench_low_molecular_weight_seed_proteins_are_restricted_to_the_embryo_and_are_not_detectable_in_the_endosperm"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="93871188" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/93871188/Screening_and_Selection_of_Hypoallergenic_Buckwheat_Species">Screening and Selection of Hypoallergenic Buckwheat Species</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="45791640" href="https://independent.academia.edu/arunnair41">arun nair</a></div><p class="ds-related-work--metadata ds2-5-body-xs">The Scientific World JOURNAL, 2002</p><p class="ds-related-work--abstract ds2-5-body-sm">Both common buckwheat (Fagopyrum esculentum) flour and meal cause an allergy in sensitive patients, and if unnoticed, it can be fatal. It has become a potential occupational hazard for some mill workers. The development of hypoallergenic buckwheat would be more efficient if natural mutants for allergenic protein are detected. A screening and selection method was developed using SDS-PAGE coupled with PCR techniques. SDS-PAGE analysis of 14 different species of buckwheat revealed thatF. lineareandF. urophyllumlack the 22-kDa major allergenic protein. PCR-based screening with specific primers for sequences encoding the allergenic protein was also effective in distinguishing the allergen-deficient species.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Screening and Selection of Hypoallergenic Buckwheat Species","attachmentId":96488000,"attachmentType":"pdf","work_url":"https://www.academia.edu/93871188/Screening_and_Selection_of_Hypoallergenic_Buckwheat_Species","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/93871188/Screening_and_Selection_of_Hypoallergenic_Buckwheat_Species"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":42757084,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":42757084,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_42757084" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. 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ds2-5-body-link" data-author-id="94193870" href="https://independent.academia.edu/RWitkowicz">Robert Witkowicz</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Journal of Cereal Science, 2016</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Basic chemical composition and bioactive compounds content in selected cultivars of buckwheat whole seeds, dehulled seeds and hulls","attachmentId":113400306,"attachmentType":"pdf","work_url":"https://www.academia.edu/117580599/Basic_chemical_composition_and_bioactive_compounds_content_in_selected_cultivars_of_buckwheat_whole_seeds_dehulled_seeds_and_hulls","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link 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