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(PDF) Genetic Diversity of Xanthomonas Citri Subsp. Citri, Causal Agent of Citrus Canker | YAHYA BIN AWANG / AGRI - Academia.edu
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window.loswp.shouldDetectTimezone = true; window.loswp.shouldShowBulkDownload = true; window.loswp.showSignupCaptcha = false window.loswp.willEdgeCache = false; window.loswp.work = {"work":{"id":33880483,"created_at":"2017-07-14T02:18:26.921-07:00","from_world_paper_id":163123874,"updated_at":"2024-11-14T10:59:54.884-08:00","_data":{"grobid_abstract":"A total of 25 samples of canker disease from different part of West Malaysia were isolated from three different hosts. After various diagnostic tests, the samples were identified as Xanthomonas citri subsp. citri (Xcc), and were also pathogenic to four tested citrus species. Molecular characterization using rep-PCR fingerprinting was carried out on the isolates. Cluster analysis using the combined banding patterns of ERIC and BOX-PCR clearly divided the isolates into different clusters according to their geographical origin, but not to their host species. A relatively high amount of genetic diversity was observed among isolates, as a group of isolates from a more restricted part of Malaysia separated from the rest with relatively low similarity, indicating that there might be distinct pathotypes of the bacterium present in Malaysia.","grobid_abstract_attachment_id":"53856243"},"document_type":"paper","pre_hit_view_count_baseline":null,"quality":"high","language":"en","title":"Genetic Diversity of Xanthomonas Citri Subsp. Citri, Causal Agent of Citrus Canker","broadcastable":true,"draft":null,"has_indexable_attachment":true,"indexable":true}}["work"]; window.loswp.workCoauthors = [66430774]; window.loswp.locale = "en"; window.loswp.countryCode = "SG"; window.loswp.cwvAbTestBucket = ""; window.loswp.designVariant = "ds_vanilla"; window.loswp.fullPageMobileSutdModalVariant = "full_page_mobile_sutd_modal"; window.loswp.useOptimizedScribd4genScript = false; window.loswp.appleClientId = 'edu.academia.applesignon';</script><script defer="" src="https://accounts.google.com/gsi/client"></script><div class="ds-loswp-container"><div class="ds-work-card--grid-container"><div class="ds-work-card--container js-loswp-work-card"><div class="ds-work-card--cover"><div class="ds-work-cover--wrapper"><div class="ds-work-cover--container"><button class="ds-work-cover--clickable js-swp-download-button" data-signup-modal="{"location":"swp-splash-paper-cover","attachmentId":53856243,"attachmentType":"pdf"}"><img alt="First page of “Genetic Diversity of Xanthomonas Citri Subsp. Citri, Causal Agent of Citrus Canker”" class="ds-work-cover--cover-thumbnail" src="https://0.academia-photos.com/attachment_thumbnails/53856243/mini_magick20190119-31541-1vlvpw3.png?1547897565" /><img alt="PDF Icon" class="ds-work-cover--file-icon" src="//a.academia-assets.com/assets/single_work_splash/adobe.icon-574afd46eb6b03a77a153a647fb47e30546f9215c0ee6a25df597a779717f9ef.svg" /><div class="ds-work-cover--hover-container"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span><p>Download Free PDF</p></div><div class="ds-work-cover--ribbon-container">Download Free PDF</div><div class="ds-work-cover--ribbon-triangle"></div></button></div></div></div><div class="ds-work-card--work-information"><h1 class="ds-work-card--work-title">Genetic Diversity of Xanthomonas Citri Subsp. Citri, Causal Agent of Citrus Canker</h1><div class="ds-work-card--work-authors ds-work-card--detail"><a class="ds-work-card--author js-wsj-grid-card-author ds2-5-body-md ds2-5-body-link" data-author-id="66430774" href="https://upm.academia.edu/YAHYABINAWANGAGRI"><img alt="Profile image of YAHYA BIN AWANG / AGRI" class="ds-work-card--author-avatar" src="https://0.academia-photos.com/66430774/29535832/27477985/s65_yahya_bin_awang._agri.jpg" />YAHYA BIN AWANG / AGRI</a></div><div class="ds-work-card--button-container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--work-card","attachmentId":53856243,"attachmentType":"pdf","workUrl":"https://www.academia.edu/33880483/Genetic_Diversity_of_Xanthomonas_Citri_Subsp_Citri_Causal_Agent_of_Citrus_Canker"}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--work-card","attachmentId":53856243,"attachmentType":"pdf","workUrl":"https://www.academia.edu/33880483/Genetic_Diversity_of_Xanthomonas_Citri_Subsp_Citri_Causal_Agent_of_Citrus_Canker"}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div></div><div data-auto_select="false" data-client_id="331998490334-rsn3chp12mbkiqhl6e7lu2q0mlbu0f1b" data-doc_id="53856243" data-landing_url="https://www.academia.edu/33880483/Genetic_Diversity_of_Xanthomonas_Citri_Subsp_Citri_Causal_Agent_of_Citrus_Canker" data-login_uri="https://www.academia.edu/registrations/google_one_tap" data-moment_callback="onGoogleOneTapEvent" id="g_id_onload"></div><div class="ds-top-related-works--grid-container"><div class="ds-related-content--container ds-top-related-works--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="0" data-entity-id="63667873" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/63667873/Evaluation_of_Phenotypic_and_Genotypic_Diversity_of_Iranian_strains_of_Xanthomonas_citri_subsp_citri_by_RAPD_PCR_in_Fars_Province">Evaluation of Phenotypic and Genotypic Diversity of Iranian strains of Xanthomonas citri subsp citri by RAPD-PCR in Fars Province</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="167777599" href="https://independent.academia.edu/KAyazpour">Kavous Ayazpour</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2014</p><p class="ds-related-work--abstract ds2-5-body-sm">Citrus bacterial canker is one of the most important citrus diseases caused by Xanthomonas citri subsp citri (Xcc). To assess the phenotypic and genotypic diversity of Xcc, 30 strains were isolated from infected areas of Fars province. All isolates were studied for physiological, biochemical and nutritional characters, host range and the existence of rpf gene using XAC02 and XAC01 primers. All strains were compared using seven RAPD primers. All strains were rod-shaped, motile, Gram-negative and obligative aerobic. They were negative in oxidase reaction but positive in catalase and hypersensitive reaction on tobacco. Using NTsys-PC software, numerical analysis of phenotypic characteristics showed that isolates are similar to each other at level 95%. In pathogenicity tests on limes (Citrus aurantifolia), all isolates showed typical symptoms of the disease after two weeks. In RAPD-PCR, using compositional analysis of seven random primers, two main groups at 75% similarity level were ob...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Evaluation of Phenotypic and Genotypic Diversity of Iranian strains of Xanthomonas citri subsp citri by RAPD-PCR in Fars Province","attachmentId":76020984,"attachmentType":"pdf","work_url":"https://www.academia.edu/63667873/Evaluation_of_Phenotypic_and_Genotypic_Diversity_of_Iranian_strains_of_Xanthomonas_citri_subsp_citri_by_RAPD_PCR_in_Fars_Province","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/63667873/Evaluation_of_Phenotypic_and_Genotypic_Diversity_of_Iranian_strains_of_Xanthomonas_citri_subsp_citri_by_RAPD_PCR_in_Fars_Province"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="1" data-entity-id="117954842" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/117954842/Genetic_Relationship_among_Worldwide_Strains_of_Xanthomonas_Causing_Canker_in_Citrus_Species_and_Design_of_New_Primers_for_Their_Identification_by_PCR">Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="33304568" href="https://independent.academia.edu/CuberoJ">J. Cubero</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Applied and Environmental Microbiology, 2002</p><p class="ds-related-work--abstract ds2-5-body-sm">Partial sequence analysis of the ribosomal operon in Xanthomonas axonopodis allowed discrimination among strains causing the A, B, and C types of citrus bacterial canker (CBC) and quantification of the relationship of these organisms with other species and pathovars in the same genus. Sets of primers based on sequence differences in the internally transcribed spacer and on a sequence from the plasmid gene pthA involved in virulence were designed for specific identification of xanthomonads causing CBC diseases. The two sets were validated with a collection of Xanthomonas strains associated with citrus species. The primer set based on ribosomal sequences had a high level of specificity for X. axonopodis pv. citri, whereas the set based on the pthA gene was universal for all types of CBC organisms. Moreover, the relationships among worldwide Xanthomonas strains causing CBC were analyzed by amplification of repetitive sequences (enterobacterial repetitive intergenic consensus and BOX el...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR","attachmentId":113691574,"attachmentType":"pdf","work_url":"https://www.academia.edu/117954842/Genetic_Relationship_among_Worldwide_Strains_of_Xanthomonas_Causing_Canker_in_Citrus_Species_and_Design_of_New_Primers_for_Their_Identification_by_PCR","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/117954842/Genetic_Relationship_among_Worldwide_Strains_of_Xanthomonas_Causing_Canker_in_Citrus_Species_and_Design_of_New_Primers_for_Their_Identification_by_PCR"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="2" data-entity-id="73857536" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/73857536/tale_Based_Genetic_Diversity_of_Chinese_Isolates_of_the_Citrus_Canker_Pathogen_Xanthomonas_citri_subsp_citri">tale -Based Genetic Diversity of Chinese Isolates of the Citrus Canker Pathogen Xanthomonas citri subsp. citri</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="26180" href="https://sjtu.academia.edu/MuhammadZakria">Muhammad Zakria</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Plant Disease, 2013</p><p class="ds-related-work--abstract ds2-5-body-sm">Pathotype A of Xanthomonas citri subsp. citri, the cause of citrus bacterial canker (CBC), is assumed to have originated in southern China. PthA, a type III secreted transcriptional activator-like effector (TALE), is a major pathogenicity determinant in X. citri subsp. citri. To investigate the diversity of X. citri subsp. citri in China, genomic and plasmid DNA of 105 X. citri subsp. citri isolates, collected from nine citrus-growing provinces of China, were digested by BamHI and hybridized with an internal repeat region of pthA. Strains were classified into 14 different genotypes (designated A to N) based on the number and size of pthA homologues. Genotypes B and G represented 19 and 62% of the isolate collection, respectively. Genotypes J and L lacked pthA or a pthA-hybridizing fragment and were less virulent on grapefruit (C. paradisi) and sweet orange (C. sinensis) compared with strains containing pthA or a pthA homologue. The virulence of genotypes J and L was increased when t...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"tale -Based Genetic Diversity of Chinese Isolates of the Citrus Canker Pathogen Xanthomonas citri subsp. citri","attachmentId":83828251,"attachmentType":"pdf","work_url":"https://www.academia.edu/73857536/tale_Based_Genetic_Diversity_of_Chinese_Isolates_of_the_Citrus_Canker_Pathogen_Xanthomonas_citri_subsp_citri","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/73857536/tale_Based_Genetic_Diversity_of_Chinese_Isolates_of_the_Citrus_Canker_Pathogen_Xanthomonas_citri_subsp_citri"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="3" data-entity-id="8802967" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/8802967/Distribution_and_pathotype_identification_of_Xanthomonas_citri_subsp_citri_recovered_from_south_western">Distribution and pathotype identification of Xanthomonas citri subsp. citri recovered from south-western</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="7940163" href="https://king-saud.academia.edu/AmgadSaleh">Amgad Saleh</a></div><p class="ds-related-work--abstract ds2-5-body-sm">We investigated the distribution of Xanthomonas citri subsp. citri (Xcc) pathotypes in south-western region of Saudi Arabia. A total of 76 Xanthomonas like strains were isolated from different citrus species showing bacterial canker symptoms from citrus commercial farms, backyard orchards and nurseries. These strains were subjected to biochemical, molecular and pathogenicity tests on leaves of grapefruit (Citrus paradisi). Based on symptoms induced on leaves of grapefruit, these strains were divided into two groups. There were 21 strains that induced typical erumpent canker lesions with watersoaked margin, belonging to Xcc type A. On the other hand, strains that produced flat necrotic lesions with water-soaked margin on grapefruit leaves belonged to Xcc A* type (55 strains). The physiological and biochemical tests, ImmuneStrip ® assays and 16S rDNA analysis confirmed the identity of the two Xcc pathotypes: A and A*. Our results indicate that, the two pathogenic variants co-exist in southwestern region of Saudi Arabia and this could further favour the generation of new genetic variants through recombination and horizontal genetic exchange. The generation of new aggressive pathotypes may threaten the citrus plantation in Saudi Arabia.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Distribution and pathotype identification of Xanthomonas citri subsp. citri recovered from south-western","attachmentId":35150275,"attachmentType":"pdf","work_url":"https://www.academia.edu/8802967/Distribution_and_pathotype_identification_of_Xanthomonas_citri_subsp_citri_recovered_from_south_western","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/8802967/Distribution_and_pathotype_identification_of_Xanthomonas_citri_subsp_citri_recovered_from_south_western"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="4" data-entity-id="111790725" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/111790725/Detection_of_viable_Xanthomonas_citri_pv_citri_the_causal_agent_of_citrus_canker_in_commercial_fruits_by_isolation_and_PCR_based_methods">Detection of viable Xanthomonas citri pv. citri, the causal agent of citrus canker in commercial fruits by isolation and PCR-based methods</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="7940163" href="https://king-saud.academia.edu/AmgadSaleh">Amgad Saleh</a></div><p class="ds-related-work--metadata ds2-5-body-xs">2015</p><p class="ds-related-work--abstract ds2-5-body-sm">Xanthomonas citri pv. citri ( Xcc ), the causal agent of citrus canker, is a quarantine pest in many citrus-growing countries and is strictly regulated by the international phytosanitary programs. Citrus fruits with canker-like symptoms imported from India and Pakistan were collected from Riyadh supermarkets, Saudi Arabia. Different diagnostic detection methods, including culturing, direct PCR, BIO-PCR and bioassays were applied on the sampled citrus fruits. The direct culturing on KCB medium succeeded in detecting living cells of Xcc in 49 out of the 217 lesions observed on the sampled citrus fruits. However, only 5.55 and 24.77% from lesion washates of Pakistani and Indian fruits, respectively, caused citrus canker lesions on the inoculated grapefruit and/or Mexican lime leaves compared with 100% from pure cultures. The application of 3 diagnostic primer sets (2/3, J-pth1/2 and Xac01/Xac02) confirmed the bacterial etiology of 83 out of the 217 lesions. Based on BIO-PCR assay, ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Detection of viable Xanthomonas citri pv. citri, the causal agent of citrus canker in commercial fruits by isolation and PCR-based methods","attachmentId":109222090,"attachmentType":"pdf","work_url":"https://www.academia.edu/111790725/Detection_of_viable_Xanthomonas_citri_pv_citri_the_causal_agent_of_citrus_canker_in_commercial_fruits_by_isolation_and_PCR_based_methods","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/111790725/Detection_of_viable_Xanthomonas_citri_pv_citri_the_causal_agent_of_citrus_canker_in_commercial_fruits_by_isolation_and_PCR_based_methods"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="5" data-entity-id="80533601" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/80533601/Distribution_and_pathotype_identification_of_Xanthomonas_citri_subsp_citri_recovered_from_southwestern_region_of_Saudi_Arabia">Distribution and pathotype identification of Xanthomonas citri subsp. citri recovered from southwestern region of Saudi Arabia</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="7940163" href="https://king-saud.academia.edu/AmgadSaleh">Amgad Saleh</a></div><p class="ds-related-work--metadata ds2-5-body-xs">African journal of microbiology research</p><p class="ds-related-work--abstract ds2-5-body-sm">We investigated the distribution of Xanthomonas citri subsp. citri (Xcc) pathotypes in south-western region of Saudi Arabia. A total of 76 Xanthomonas like strains were isolated from different citrus species showing bacterial canker symptoms from citrus commercial farms, backyard orchards and nurseries. These strains were subjected to biochemical, molecular and pathogenicity tests on leaves of grapefruit (Citrus paradisi). Based on symptoms induced on leaves of grapefruit, these strains were divided into two groups. There were 21 strains that induced typical erumpent canker lesions with watersoaked margin, belonging to Xcc type A. On the other hand, strains that produced flat necrotic lesions with water-soaked margin on grapefruit leaves belonged to Xcc A* type (55 strains). The physiological and biochemical tests, ImmuneStrip ® assays and 16S rDNA analysis confirmed the identity of the two Xcc pathotypes: A and A*. Our results indicate that, the two pathogenic variants co-exist in ...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Distribution and pathotype identification of Xanthomonas citri subsp. citri recovered from southwestern region of Saudi Arabia","attachmentId":86883430,"attachmentType":"pdf","work_url":"https://www.academia.edu/80533601/Distribution_and_pathotype_identification_of_Xanthomonas_citri_subsp_citri_recovered_from_southwestern_region_of_Saudi_Arabia","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/80533601/Distribution_and_pathotype_identification_of_Xanthomonas_citri_subsp_citri_recovered_from_southwestern_region_of_Saudi_Arabia"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="6" data-entity-id="50579433" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/50579433/Molecular_Diversity_among_the_Isolates_of_Xanthomonas_axonopodis_pv_citri_Causing_Bacterial_Canker_in_Citrus">Molecular Diversity among the Isolates of Xanthomonas axonopodis pv. citri Causing Bacterial Canker in Citrus</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="144810350" href="https://independent.academia.edu/BhausahebGhorpade">Bhausaheb Ghorpade</a></div><p class="ds-related-work--abstract ds2-5-body-sm">Citrus has an important value as fruit crop. Present day citrus is delectable, juicy, and seedless is of great nutritional significance as well, additionally, it possesses enormous therapeutic qualities. Citrus canker caused by Xanthomonas axonopodis pv.citri is a damaging disease of acid lime. The bacterium infects the twigs, petioles, fruit stalks and fruits, resulting in both qualitative and quantitative loss in acid lime. The isolations and identification were made from the symptomatic samples collected from different districts, viz. Ahmednagar (XacI), Pune (XacII), solapur (XacIII), Nashik (XacIV), Belgaum (XacV), and grouped as per agro-ecological zones. The growth characteristics of all the isolates were studied on nutrient agar medium.Through morphological, biochemical and pathogenicity test isolates were identified and confirmed as Xanthomonas axonopodis pv.citri.The variability was studied among the five isolates of Xac by using 14 RAPD and 08 ISSR primers.Level of polymor...</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Molecular Diversity among the Isolates of Xanthomonas axonopodis pv. citri Causing Bacterial Canker in Citrus","attachmentId":68507705,"attachmentType":"pdf","work_url":"https://www.academia.edu/50579433/Molecular_Diversity_among_the_Isolates_of_Xanthomonas_axonopodis_pv_citri_Causing_Bacterial_Canker_in_Citrus","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/50579433/Molecular_Diversity_among_the_Isolates_of_Xanthomonas_axonopodis_pv_citri_Causing_Bacterial_Canker_in_Citrus"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="7" data-entity-id="43911761" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/43911761/Diagnosis_of_Xanthomonas_axonopodis_pv_citri_isolates_the_causal_agent_of_citrus_canker_by_pathogenicity_and_PCR_based_methods">Diagnosis of Xanthomonas axonopodis pv citri isolates, the causal agent of citrus canker by pathogenicity and PCR based methods</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="3564045" href="https://independent.academia.edu/PradeepManyam">Pradeep Manyam</a></div><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Diagnosis of Xanthomonas axonopodis pv citri isolates, the causal agent of citrus canker by pathogenicity and PCR based methods","attachmentId":64236242,"attachmentType":"pdf","work_url":"https://www.academia.edu/43911761/Diagnosis_of_Xanthomonas_axonopodis_pv_citri_isolates_the_causal_agent_of_citrus_canker_by_pathogenicity_and_PCR_based_methods","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/43911761/Diagnosis_of_Xanthomonas_axonopodis_pv_citri_isolates_the_causal_agent_of_citrus_canker_by_pathogenicity_and_PCR_based_methods"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="8" data-entity-id="35359160" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/35359160/Genetic_and_Pathological_Diversity_Among_Xanthomonas_axonopodis_citri_Strains_Causing_Citrus_Canker_in_Maharashtra_India">Genetic and Pathological Diversity Among Xanthomonas axonopodis citri Strains Causing Citrus Canker in Maharashtra, India</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="17497850" href="https://independent.academia.edu/MArshiya">Minhaj Arshiya</a></div><p class="ds-related-work--abstract ds2-5-body-sm">The present study is based on biochemical characterization, pathogenicity test and DNA based technique. Here apart from biochemical test, RAPD analysis was used to distinguish and differentiate among 24 strains of Xac isolated from Marathwada region in Maharashtra. 200 strains of Xac isolated from Citrus lemona from different location within Marathwada region of Maharashtra and 24 strains representing all the 8 districts were used in this study. All these strains were purified and maintained on YDC (Yeast extract-1gm, D-glucose-2gm, Calcium carbonate-2gm) slants. The presently used primer could resolve genetic diversity and geographical distribution in the study of Xanthomonas axonopodis pv citri and RAPD marker gave reproducible and reliable results.</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Genetic and Pathological Diversity Among Xanthomonas axonopodis citri Strains Causing Citrus Canker in Maharashtra, India","attachmentId":55219432,"attachmentType":"pdf","work_url":"https://www.academia.edu/35359160/Genetic_and_Pathological_Diversity_Among_Xanthomonas_axonopodis_citri_Strains_Causing_Citrus_Canker_in_Maharashtra_India","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/35359160/Genetic_and_Pathological_Diversity_Among_Xanthomonas_axonopodis_citri_Strains_Causing_Citrus_Canker_in_Maharashtra_India"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-wsj-grid-card" data-collection-position="9" data-entity-id="60825231" data-sort-order="default"><a class="ds-related-work--title js-wsj-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/60825231/Genomics_of_Xanthomonas_citri_and_Related_Species">Genomics of Xanthomonas citri and Related Species</a><div class="ds-related-work--metadata"><a class="js-wsj-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="17121767" href="https://independent.academia.edu/JalanN">Neha Jalan</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Genomics of Plant-Associated Bacteria, 2014</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Genomics of Xanthomonas citri and Related Species","attachmentId":74098478,"attachmentType":"pdf","work_url":"https://www.academia.edu/60825231/Genomics_of_Xanthomonas_citri_and_Related_Species","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-wsj-grid-card-view-pdf" href="https://www.academia.edu/60825231/Genomics_of_Xanthomonas_citri_and_Related_Species"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div></div></div><div class="ds-sticky-ctas--wrapper js-loswp-sticky-ctas hidden"><div class="ds-sticky-ctas--grid-container"><div class="ds-sticky-ctas--container"><button class="ds2-5-button js-swp-download-button" data-signup-modal="{"location":"continue-reading-button--sticky-ctas","attachmentId":53856243,"attachmentType":"pdf","workUrl":null}">See full PDF</button><button class="ds2-5-button ds2-5-button--secondary js-swp-download-button" data-signup-modal="{"location":"download-pdf-button--sticky-ctas","attachmentId":53856243,"attachmentType":"pdf","workUrl":null}"><span class="material-symbols-outlined" style="font-size: 20px" translate="no">download</span>Download PDF</button></div></div></div><div class="ds-below-fold--grid-container"><div class="ds-work--container js-loswp-embedded-document"><div class="attachment_preview" data-attachment="Attachment_53856243" style="display: none"><div class="js-scribd-document-container"><div class="scribd--document-loading js-scribd-document-loader" style="display: block;"><img alt="Loading..." src="//a.academia-assets.com/images/loaders/paper-load.gif" /><p>Loading Preview</p></div></div><div style="text-align: center;"><div class="scribd--no-preview-alert js-preview-unavailable"><p>Sorry, preview is currently unavailable. You can download the paper by clicking the button above.</p></div></div></div></div><div class="ds-sidebar--container js-work-sidebar"><div class="ds-related-content--container"><h2 class="ds-related-content--heading">Related papers</h2><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="0" data-entity-id="8525710" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/8525710/Repetitive_PCR_based_detection_of_Genetic_Diversity_in_Xanthomonas_axonopodis_pv_citri_Strains">Repetitive PCR based detection of Genetic Diversity in Xanthomonas axonopodis pv citri Strains</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="17497850" href="https://independent.academia.edu/MArshiya">Minhaj Arshiya</a></div><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Repetitive PCR based detection of Genetic Diversity in Xanthomonas axonopodis pv citri Strains","attachmentId":34903654,"attachmentType":"pdf","work_url":"https://www.academia.edu/8525710/Repetitive_PCR_based_detection_of_Genetic_Diversity_in_Xanthomonas_axonopodis_pv_citri_Strains","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/8525710/Repetitive_PCR_based_detection_of_Genetic_Diversity_in_Xanthomonas_axonopodis_pv_citri_Strains"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="1" data-entity-id="33976821" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/33976821/GENETIC_DIVERSITY_AMONG_XANTHOMONAS_CITRI_SUBSP_CITRI_STRAINS_IN_IRAN">GENETIC DIVERSITY AMONG XANTHOMONAS CITRI SUBSP. CITRI STRAINS IN IRAN</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="66598888" href="https://independent.academia.edu/mohannasoleymanpour">mohanna soleymanpour</a></div><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"GENETIC DIVERSITY AMONG XANTHOMONAS CITRI SUBSP. CITRI STRAINS IN IRAN","attachmentId":53930061,"attachmentType":"pdf","work_url":"https://www.academia.edu/33976821/GENETIC_DIVERSITY_AMONG_XANTHOMONAS_CITRI_SUBSP_CITRI_STRAINS_IN_IRAN","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/33976821/GENETIC_DIVERSITY_AMONG_XANTHOMONAS_CITRI_SUBSP_CITRI_STRAINS_IN_IRAN"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="2" data-entity-id="92787263" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/92787263/Genetic_diversity_of_Xanthomonas_citri_subsp_citri_in_citrus_orchards_in_northwest_Paran%C3%A1_state_Brazil">Genetic diversity of Xanthomonas citri subsp. citri in citrus orchards in northwest Paraná state, Brazil</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="38464026" href="https://independent.academia.edu/CarlosAlexandreZanutto">Carlos Alexandre Zanutto</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Phytopathologia Mediterranea, 2017</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Genetic diversity of Xanthomonas citri subsp. citri in citrus orchards in northwest Paraná state, Brazil","attachmentId":95703870,"attachmentType":"pdf","work_url":"https://www.academia.edu/92787263/Genetic_diversity_of_Xanthomonas_citri_subsp_citri_in_citrus_orchards_in_northwest_Paran%C3%A1_state_Brazil","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/92787263/Genetic_diversity_of_Xanthomonas_citri_subsp_citri_in_citrus_orchards_in_northwest_Paran%C3%A1_state_Brazil"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="3" data-entity-id="26174944" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/26174944/Genetic_Diversity_and_Pathogenicity_of_Xanthomonas_axonopodis_Strains_Inducing_Citrus_Canker_Disease_in_Iran_and_South_Korea">Genetic Diversity and Pathogenicity of Xanthomonas axonopodis Strains Inducing Citrus Canker Disease in Iran and South Korea</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="32740961" href="https://ugent.academia.edu/JSwings">J. Swings</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Indian Journal of Microbiology, 2011</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Genetic Diversity and Pathogenicity of Xanthomonas axonopodis Strains Inducing Citrus Canker Disease in Iran and South Korea","attachmentId":46501131,"attachmentType":"pdf","work_url":"https://www.academia.edu/26174944/Genetic_Diversity_and_Pathogenicity_of_Xanthomonas_axonopodis_Strains_Inducing_Citrus_Canker_Disease_in_Iran_and_South_Korea","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/26174944/Genetic_Diversity_and_Pathogenicity_of_Xanthomonas_axonopodis_Strains_Inducing_Citrus_Canker_Disease_in_Iran_and_South_Korea"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="4" data-entity-id="53124178" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/53124178/Recent_advances_in_the_understanding_of_Xanthomonas_citri_ssp_citri_pathogenesis_and_citrus_canker_disease_management">Recent advances in the understanding of Xanthomonas citri ssp. citri pathogenesis and citrus canker disease management</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="64890966" href="https://independent.academia.edu/FranklinBehlau">Franklin Behlau</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Molecular plant pathology, 2018</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Recent advances in the understanding of Xanthomonas citri ssp. citri pathogenesis and citrus canker disease management","attachmentId":70058761,"attachmentType":"pdf","work_url":"https://www.academia.edu/53124178/Recent_advances_in_the_understanding_of_Xanthomonas_citri_ssp_citri_pathogenesis_and_citrus_canker_disease_management","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" 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href="https://independent.academia.edu/NKositcharoenkul">Nuttima Kositcharoenkul</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Plant Pathology, 2011</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Detection of Xanthomonas citri subsp. citri from field samples using single-tube nested PCR","attachmentId":71350127,"attachmentType":"pdf","work_url":"https://www.academia.edu/55515563/Detection_of_Xanthomonas_citri_subsp_citri_from_field_samples_using_single_tube_nested_PCR","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" 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ds2-5-body-xs">2023</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"Epidemiological Markers for Citrus Canker caused by Xanthomonas citri pv. citri","attachmentId":116929586,"attachmentType":"pdf","work_url":"https://www.academia.edu/122217486/Epidemiological_Markers_for_Citrus_Canker_caused_by_Xanthomonas_citri_pv_citri","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/122217486/Epidemiological_Markers_for_Citrus_Canker_caused_by_Xanthomonas_citri_pv_citri"><span class="ds2-5-text-link__content">View PDF</span><span class="material-symbols-outlined" style="font-size: 18px" translate="no">chevron_right</span></a></div></div><div class="ds-related-work--container js-related-work-sidebar-card" data-collection-position="7" data-entity-id="91992179" data-sort-order="default"><a class="ds-related-work--title js-related-work-grid-card-title ds2-5-body-md ds2-5-body-link" href="https://www.academia.edu/91992179/Pathological_Bio_chemical_and_Molecular_diversity_amongst_the_isolates_of_Xanthomonas_axonopodis_pv_citri_causing_Citrus_canker_in_acid_lime_from_different_agro_climatic_region_of_India">Pathological, Bio-chemical and Molecular diversity amongst the isolates of Xanthomonas axonopodis pv. citri causing Citrus canker in acid lime from different agro-climatic region of India</a><div class="ds-related-work--metadata"><a class="js-related-work-grid-card-author ds2-5-body-sm ds2-5-body-link" data-author-id="2863370" href="https://mpkv.academia.edu/VivekChimote">Vivek Chimote</a></div><p class="ds-related-work--metadata ds2-5-body-xs">International Journal of 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Hailstones</a></div><p class="ds-related-work--metadata ds2-5-body-xs">Australasian Plant Disease Notes, 2015</p><div class="ds-related-work--ctas"><button class="ds2-5-text-link ds2-5-text-link--inline js-swp-download-button" data-signup-modal="{"location":"wsj-grid-card-download-pdf-modal","work_title":"First record of citrus canker, caused by Xanthomonas citri subsp. citri in Solomon Islands","attachmentId":52282964,"attachmentType":"pdf","work_url":"https://www.academia.edu/32017069/First_record_of_citrus_canker_caused_by_Xanthomonas_citri_subsp_citri_in_Solomon_Islands","alternativeTracking":true}"><span class="material-symbols-outlined" style="font-size: 18px" translate="no">download</span><span class="ds2-5-text-link__content">Download free PDF</span></button><a class="ds2-5-text-link ds2-5-text-link--inline js-related-work-grid-card-view-pdf" href="https://www.academia.edu/32017069/First_record_of_citrus_canker_caused_by_Xanthomonas_citri_subsp_citri_in_Solomon_Islands"><span 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