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Leptospira</title> <meta name="description" content="Search results for: Leptospira"> <meta name="keywords" content="Leptospira"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="Leptospira"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 7</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Leptospira</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Isolation of the Leptospira spp. from the Rice Farming Lands in the North of Iran by EMJH Media</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Rostampour%20Yasouri">S. Rostampour Yasouri</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Ghane"> M. Ghane</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leptospirosis is one the most important common diseases between human and live stock occurred by different species of Leptospira. This disease has been construed as the native in the northern provinces of Iran and risk of the infection with pathogenic is high. One hundred fifteen samples of water (67), soil (36) and feces of rodents (12) were collected from the rice fields of the suburbs of Tonekabon Township situated in northern part of Iran in 2012. The samples, after passage from membranous filters, were cultured in the liquid and solid EMJH medium and incubated at 30°C for 1 month. Leptospira spp. were isolated using culture technique, and the plates were studied from viewpoint of colony formation, microscopic observations and then identified by phenotyping tests. Finally, the identification of Leptospira genus was verified by PCR technique and 16S rRNA gene sequencing. Of 115 samples totally, 55 samples (47.82%) became positive by use of the culture technique which the positive cases included 47 water samples (70.14%) and 8 soil samples (22.22%), while the isolation was not accomplished from the sample of the rodents feces. Overall, according to these data, Leptospira spp. exists with high frequency in North Iran. Hence, based on foregoing evidence environments in the north of Iran are vehicles of Leptospira spp. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=EMJH%20Medium" title="EMJH Medium">EMJH Medium</a>, <a href="https://publications.waset.org/abstracts/search?q=Leptospira" title=" Leptospira"> Leptospira</a>, <a href="https://publications.waset.org/abstracts/search?q=Northern%20of%20Iran" title=" Northern of Iran"> Northern of Iran</a>, <a href="https://publications.waset.org/abstracts/search?q=rice%20fields" title=" rice fields"> rice fields</a> </p> <a href="https://publications.waset.org/abstracts/75162/isolation-of-the-leptospira-spp-from-the-rice-farming-lands-in-the-north-of-iran-by-emjh-media" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/75162.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">179</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Positivity of Pathogenic Leptospira in Pigs from Rural Communities on the Coast of Ecuador </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Veronica%20Barragan">Veronica Barragan</a>, <a href="https://publications.waset.org/abstracts/search?q=Ligia%20Luna"> Ligia Luna</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20Patricia%20Zambrano"> Maria Patricia Zambrano</a>, <a href="https://publications.waset.org/abstracts/search?q=Carlos%20%20Bulnes"> Carlos Bulnes</a>, <a href="https://publications.waset.org/abstracts/search?q=Eduardo%20Diaz"> Eduardo Diaz</a>, <a href="https://publications.waset.org/abstracts/search?q=Talima%20%20Pearson"> Talima Pearson</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leptospirosis impacts animal production and is responsible for important economic losses in the pig industry. Infection is associated with reproductive failures that lead to abortions, stillbirth, and perinatal mortality. The leptospira serogroups that have been traditionally linked to disease in pigs are Pomona, Australis, and Tarassovi. Unfortunately, knowledge about pig leptospirosis is biased towards infection in large-scale commercial farms from developed countries, where exposure is usually limited to host-specific serotypes. The aim of our study is to describe leptospirosis in pigs from rural communities located in the coast of Ecuador-South America, where leptospirosis is endemic. A particularity of these pigs is that, because they are usually raised in the backyard of their owner’s houses, exposure to other leptospira excreted by other animals is likely to occur. Therefore, we collected 420 kidney samples from pigs sacrificed at a local slaughterhouse, and Leptospira positivity was tested in all samples by amplifying the Lipl32 gen. Our results show pathogenic Leptospira positivity in 19.3% (81/420) of pigs. Microaglutination test was performed in 60 PCR positive samples with titers >1:100 in 17 pigs, titers of 1:50 in 28 pigs, and no MAT titers in 15 pigs even though Leptospira DNA was found in their kidneys. Interestingly, reacting serovars were very diverse, with 18.3% of pig sera reacting with two or more serovars. Additionally, serovar Canicola was found in 16.7% of pigs followed by Tarassovi (10%), Australis (6.7%), Pyogenes (5%), Icterohaemorrhageae (1.7%), and Grippotyphosa (1.7%). It is also important to highlight that most of the analyzed animals came from small-scale farms where pigs may be exposed to the pathogen by exposure to other domestic and peridomestic animals such as rats, dogs, horses, donkeys, and even wildlife. This would explain the finding of non-pig adapted Leptospira serovars such as Canicola, which is commonly reported in dogs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Leptospira" title="Leptospira">Leptospira</a>, <a href="https://publications.waset.org/abstracts/search?q=Lipl32" title=" Lipl32"> Lipl32</a>, <a href="https://publications.waset.org/abstracts/search?q=peridomestic" title=" peridomestic"> peridomestic</a>, <a href="https://publications.waset.org/abstracts/search?q=pig" title=" pig"> pig</a>, <a href="https://publications.waset.org/abstracts/search?q=serovar" title=" serovar"> serovar</a> </p> <a href="https://publications.waset.org/abstracts/124826/positivity-of-pathogenic-leptospira-in-pigs-from-rural-communities-on-the-coast-of-ecuador" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/124826.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">138</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Investigation of Leptospira Infection in Stray Animals in Thailand: Leptospirosis Risk Reduction in Human</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ruttayaporn%20Ngasaman">Ruttayaporn Ngasaman</a>, <a href="https://publications.waset.org/abstracts/search?q=Saowakon%20Indouang"> Saowakon Indouang</a>, <a href="https://publications.waset.org/abstracts/search?q=Usa%20Chethanond"> Usa Chethanond</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leptospirosis is a public health concern zoonosis in Thailand. Human and animals are often infected by contact with contaminated water. The infected animals play an important role in leptospira infection for both human and other hosts via urine. In humans, it can cause a wide range of symptoms, some of which may present mild flu-like symptoms including fever, vomiting, and jaundice. Without treatment, Leptospirosis can lead to kidney damage, meningitis, liver failure, respiratory distress, and even death. The prevalence of leptospirosis in stray animals in Thailand is unknown. The aim of this study was to investigate leptospira infection in stray animals including dogs and cats in Songkhla province, Thailand. Total of 434 blood samples were collected from 370 stray dogs and 64 stray cats during the population control program from 2014 to 2018. Screening test using latex agglutination for the detection of antibodies against Leptospira interrogans in serum samples shows 29.26% (127/434) positive. There were 120 positive samples of stray dogs and 7 positive samples of stray cats. Detection by polymerase chain reaction specific to LipL32 gene of Leptospira interrogans showed 1.61% (7/434) positive. Stray cats (5/64) show higher prevalence than stray dogs (2/370). Although active infection was low detected, but seroprevalence was high. This result indicated that stray animals were not active infection during sample collection but they use to get infected or in a latent period of infection. They may act as a reservoir for domestic animals and human in which stay in the same environment. In order to prevent and reduce the risk of leptospira infection in a human, stray animals should be done health checking, vaccination, and disease treatment. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=leptospirosis" title="leptospirosis">leptospirosis</a>, <a href="https://publications.waset.org/abstracts/search?q=stray%20animals" title=" stray animals"> stray animals</a>, <a href="https://publications.waset.org/abstracts/search?q=risk%20reduction" title=" risk reduction"> risk reduction</a>, <a href="https://publications.waset.org/abstracts/search?q=Thailand" title=" Thailand"> Thailand</a> </p> <a href="https://publications.waset.org/abstracts/104051/investigation-of-leptospira-infection-in-stray-animals-in-thailand-leptospirosis-risk-reduction-in-human" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/104051.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">133</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Leptospira Lipl32-Specific Antibodies: Therapeutic Property, Epitopes Characterization and Molecular Mechanisms of Neutralization</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Santi%20Maneewatchararangsri">Santi Maneewatchararangsri</a>, <a href="https://publications.waset.org/abstracts/search?q=Wanpen%20Chaicumpa"> Wanpen Chaicumpa</a>, <a href="https://publications.waset.org/abstracts/search?q=Patcharin%20Saengjaruk"> Patcharin Saengjaruk</a>, <a href="https://publications.waset.org/abstracts/search?q=Urai%20Chaisri"> Urai Chaisri </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leptospirosis is a globally neglected disease that continues to be a significant public health and veterinary burden, with millions of cases reported each year. Early and accurate differential diagnosis of leptospirosis from other febrile illnesses and the development of a broad spectrum of leptospirosis vaccines are needed. The LipL32 outer membrane lipoprotein is a member of Leptospira adhesive matrices and has been found to exert hemolytic activity to erythrocytes in vitro. Therefore, LipL32 is regarded as a potential target for diagnosis, broad-spectrum leptospirosis vaccines, and for passive immunotherapy. In this study, we established LipL32-specific mouse monoclonal antibodies, mAbLPF1 and mAbLPF2, and their respective mouse- and humanized-engineered single chain variable fragment (ScFv). Their antibodies’ neutralizing activities against Leptospira-mediated hemolysis in vitro, and the therapeutic efficacy of mAbs against heterologous Leptospira infected hamsters were demonstrated. The epitope peptide of mAb LPF1 was mapped to a non-contiguous carboxy-terminal β-turn and amphipathic α-helix of LipL32 structure contributing to phospholipid/host cell adhesion and membrane insertion. We found that the mAbLPF2 epitope was located on the interacting loop of peptide binding groove of the LipL32 molecule responsible for interactions with host constituents. Epitope sequences are highly conserved among Leptospira spp. and are absent from the LipL32 superfamily of other microorganisms. Both epitopes are surface-exposed, readily accessible by mAbs, and immunogenic. However, they are less dominant when revealed by LipL32-specific immunoglobulins from leptospirosis-patient sera and rabbit hyperimmune serum raised by whole Leptospira. Our study also demonstrated an adhesion inhibitory activity of LipL32 protein to host membrane components and cells mediated by mAbs as well as an anti-hemolytic activity of the respective antibodies. The therapeutic antibodies, particularly the humanized-ScFv, have a potential for further development as non-drug therapeutic agent for human leptospirosis, especially in subjects allergic to antibiotics. The epitope peptides recognized by two therapeutic mAbs have potential use as tools for structure-function studies. Finally, protective peptides may be used as a target for epitope-based vaccines for control of leptospirosis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=leptospira%20lipl32-specific%20antibodies" title="leptospira lipl32-specific antibodies">leptospira lipl32-specific antibodies</a>, <a href="https://publications.waset.org/abstracts/search?q=therapeutic%20epitopes" title=" therapeutic epitopes"> therapeutic epitopes</a>, <a href="https://publications.waset.org/abstracts/search?q=epitopes%20characterization" title=" epitopes characterization"> epitopes characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=immunotherapy" title=" immunotherapy"> immunotherapy</a> </p> <a href="https://publications.waset.org/abstracts/31247/leptospira-lipl32-specific-antibodies-therapeutic-property-epitopes-characterization-and-molecular-mechanisms-of-neutralization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/31247.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">297</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Rapid Identification and Diagnosis of the Pathogenic Leptospiras through Comparison among Culture, PCR and Real Time PCR Techniques from Samples of Human and Mouse Feces</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Rostampour%20Yasouri">S. Rostampour Yasouri</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Ghane"> M. Ghane</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Doudi"> M. Doudi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Leptospirosis is one of the most significant infectious and zoonotic diseases along with global spreading. This disease is causative agent of economoic losses and human fatalities in various countries, including Northern provinces of Iran. The aim of this research is to identify and compare the rapid diagnostic techniques of pathogenic leptospiras, considering the multifacetedness of the disease from a clinical manifestation and premature death of patients. In the spring and summer of 2020-2022, 25 fecal samples were collected from suspected leptospirosis patients and 25 Fecal samples from mice residing in the rice fields and factories in Tonekabon city. Samples were prepared by centrifugation and passing through membrane filters. Culture technique was used in liquid and solid EMJH media during one month of incubation at 30°C. Then, the media were examined microscopically. DNA extraction was conducted by extraction Kit. Diagnosis of leptospiras was enforced by PCR and Real time PCR (SYBR Green) techniques using lipL32 specific primer. Out of the patients, 11 samples (44%) and 8 samples (32%) were determined to be pathogenic Leptospira by Real time PCR and PCR technique, respectively. Out of the mice, 9 Samples (36%) and 3 samples (12%) were determined to be pathogenic Leptospira by the mentioned techniques, respectively. Although the culture technique is considered to be the gold standard technique, but due to the slow growth of pathogenic Leptospira and lack of colony formation of some species, it is not a fast technique. Real time PCR allowed rapid diagnosis with much higher accuracy compared to PCR because PCR could not completely identify samples with lower microbial load. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=culture" title="culture">culture</a>, <a href="https://publications.waset.org/abstracts/search?q=pathogenic%20leptospiras" title=" pathogenic leptospiras"> pathogenic leptospiras</a>, <a href="https://publications.waset.org/abstracts/search?q=PCR" title=" PCR"> PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=real%20time%20PCR" title=" real time PCR"> real time PCR</a> </p> <a href="https://publications.waset.org/abstracts/165213/rapid-identification-and-diagnosis-of-the-pathogenic-leptospiras-through-comparison-among-culture-pcr-and-real-time-pcr-techniques-from-samples-of-human-and-mouse-feces" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165213.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">85</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Detection of Leptospira interrogans in Kidney and Urine of water Buffalo and its Relationship with Histopathological and Serological Findings</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20R.%20Haji%20Hajikolaei">M. R. Haji Hajikolaei</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20A.%20Nikvand"> A. A. Nikvand</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20R.%20Ghadrdan"> A. R. Ghadrdan</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Ghorbanpoor"> M. Ghorbanpoor</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20Mohammadian"> B. Mohammadian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study was carried out on water buffalo for detection of Leptospira interrogans in kidney and urine and its relationship with serological findings. Blood, urine and kidney samples were taken immediately after slaughter from 353 water buffalos at Ahvaz abattoir in Khouzestan province, Iran. Sera were initially screened at serum dilution of 1:100 against seven live antigens of Leptospira interrogans: pomona, hardjo, ballum, icterohemorrhagiae, tarasovi, australis and grippotyphosa using the microscopic agglutination test (MAT) and sera with positive results were titrated against reacting antigens in serial twofold dilution from 1:100 to 1:800. The samples of kidney were embedded in paraffin wax and 5µm thick sections were stained routinely with Haematoxylin and Eosin (H&E). Polymerase chain reaction (PCR) examination was done on urine and kidney by using LipL32 gene primers. Antibodies against one or more serovars at dilution >:100 were detected in sera. The most frequent reactor was hardjo (56.2%), followed by pomona (52.3%), australis (9.8%), tarassovi (5.9%), grippotyphosa (4.5%) and icterohaemorrhagiae (3.9%). The L. interrogans were detected in 43 (12.2%) of examined buffaloes, so that 26 (8.2%) of kidney tissues, 14 (4.8%) of urine samples separately and 3 (0.84%) of both kidney and urine samples were positive in PCR. From 153 (43.3%) buffaloes with positive MAT, 24 cases were positive by PCR of kidney and/or urine samples, synchronously. Renal lesions such as interstitial nephritis, acute tubular necrosis (ATN), pyelonephritis, glomerolonephritis, renal fibrosis and hydronephrosis were found in 128 (36.3%) cases. Statistical analysis indicated that there was no significant association between results of MAT, PCR and interstitial nephritis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=leptospiral%20infection" title="leptospiral infection">leptospiral infection</a>, <a href="https://publications.waset.org/abstracts/search?q=PCR" title=" PCR"> PCR</a>, <a href="https://publications.waset.org/abstracts/search?q=MAT" title=" MAT"> MAT</a>, <a href="https://publications.waset.org/abstracts/search?q=histopathology" title=" histopathology"> histopathology</a>, <a href="https://publications.waset.org/abstracts/search?q=river%20buffalo" title=" river buffalo"> river buffalo</a> </p> <a href="https://publications.waset.org/abstracts/39412/detection-of-leptospira-interrogans-in-kidney-and-urine-of-water-buffalo-and-its-relationship-with-histopathological-and-serological-findings" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/39412.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">332</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Integrated Lateral Flow Electrochemical Strip for Leptospirosis Diagnosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Wanwisa%20Deenin">Wanwisa Deenin</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdulhadee%20Yakoh"> Abdulhadee Yakoh</a>, <a href="https://publications.waset.org/abstracts/search?q=Chahya%20Kreangkaiwal"> Chahya Kreangkaiwal</a>, <a href="https://publications.waset.org/abstracts/search?q=Orawon%20Chailapakul"> Orawon Chailapakul</a>, <a href="https://publications.waset.org/abstracts/search?q=Kanitha%20Patarakul"> Kanitha Patarakul</a>, <a href="https://publications.waset.org/abstracts/search?q=Sudkate%20Chaiyo"> Sudkate Chaiyo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> LipL32 is an outer membrane protein present only on pathogenic Leptospira species, which are the causative agent of leptospirosis. Leptospirosis symptoms are often misdiagnosed with other febrile illnesses as the clinical manifestations are non-specific. Therefore, an accurate diagnostic tool for leptospirosis is indeed critical for proper and prompt treatment. Typical diagnosis via serological assays is generally performed to assess the antibodies produced against Leptospira. However, their delayed antibody response and complicated procedure are undoubtedly limited the practical utilization especially in primary care setting. Here, we demonstrate for the first time an early-stage detection of LipL32 by an integrated lateral-flow immunoassay with electrochemical readout (eLFIA). A ferrocene trace tag was monitored via differential pulse voltammetry operated on a smartphone-based device, thus allowing for on-field testing. Superior performance in terms of the lowest detectable limit of detection (LOD) of 8.53 pg/mL and broad linear dynamic range (5 orders of magnitude) among other sensors available thus far was established. Additionally, the developed test strip provided a straightforward yet sensitive approach for diagnosis of leptospirosis using the collected human sera from patients, in which the results were comparable to the real-time polymerase chain reaction technique. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=leptospirosis" title="leptospirosis">leptospirosis</a>, <a href="https://publications.waset.org/abstracts/search?q=electrochemical%20detection" title=" electrochemical detection"> electrochemical detection</a>, <a href="https://publications.waset.org/abstracts/search?q=lateral%20flow%20immunosensor" title=" lateral flow immunosensor"> lateral flow immunosensor</a>, <a href="https://publications.waset.org/abstracts/search?q=point-of-care%20testing" title=" point-of-care testing"> point-of-care testing</a>, <a href="https://publications.waset.org/abstracts/search?q=early-stage%20detection" title=" early-stage detection"> early-stage detection</a> </p> <a href="https://publications.waset.org/abstracts/162411/integrated-lateral-flow-electrochemical-strip-for-leptospirosis-diagnosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/162411.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">93</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational 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