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Search results for: Crataegus azarolus

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</div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: Crataegus azarolus</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> Hypotensive, Free Radical Scavenging and Anti-Lipid Peroxidation Activities of Crataegus azarolus L. Leaves Extracts Growing in Algeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Amel%20Bouaziz">Amel Bouaziz</a>, <a href="https://publications.waset.org/abstracts/search?q=Seddik%20Khennouf"> Seddik Khennouf</a>, <a href="https://publications.waset.org/abstracts/search?q=Mussa%20Abu%20Zarga"> Mussa Abu Zarga</a>, <a href="https://publications.waset.org/abstracts/search?q=Shtayway%20Abdalla"> Shtayway Abdalla</a>, <a href="https://publications.waset.org/abstracts/search?q=Saliha%20Djidel"> Saliha Djidel</a>, <a href="https://publications.waset.org/abstracts/search?q=Assia%20Bentahar"> Assia Bentahar</a>, <a href="https://publications.waset.org/abstracts/search?q=Saliha%20Dahamna"> Saliha Dahamna</a>, <a href="https://publications.waset.org/abstracts/search?q=Smain%20Amira"> Smain Amira</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present study aimed to evaluate the hypotensive and the in vitro antioxidant activities of Crataegus azarolus L. (Rosaceae), a plant widely used as natural remedy for hypertension in folk medicine. The antioxidant potential of methanolic extract (ME)and its three fractions of Chloroform (CHE), ethyl acetate (EAE)and water (AqE) have been investigated using several assays, including the DPPH scavenging, ABTS scavenging, hydroxyl radical scavenging. Inhibition of lipid peroxidation was performed by the β-carotene bleaching assay, ferric thiocyanate method and thiobarburic acid method. Total phenolic and total flavonoid contents of the extracts were estimated using Folin-Chiocalteu reagent and AlCl3, respectively. EAE extract showed the highest polyphenolic and flavonoids contents (396,04±1.20 mg GAE/g of dry extract and 32,73 ± 0.03mg QE/g of dry extract) respectively. Similarly, this extract possessed the highest scavenging activity for DPPH radical (IC 50 = 0,006±0,0001mg /ml), ABTS radical (IC50=0.0035±0,0007 mg/ml) and hydroxyl radical(IC 50=0,283± 0.01 mg/ml). In addition, the EAE exhibited the highest antioxidant activity in the inhibition of linoleic acid/ß-carotene coupled oxidation (89,21%), lipid peroxidation in the ferric thiocyanate(FTC) method (90.13%), and thio-barbituric acid (TBA) method (74.23%). Intravenous administration of Me and EAE decreased mean arterial blood pressure, systolic and diastolic blood pressure in anesthetized rats dose-dependently, at the dose range of 0.4 to 12 mg/kg. The mean arterial blood pressure dropped by 27.58 and 39.37% for ME and EAE, respectively. In conclusion, The present study supported the significant potential to use C. azarolus by-products as a source of natural antioxidants and provides scientific justification for its traditional uses as cardio-protective and anti-hypertensive remedy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Crataegus%20azarolus" title="Crataegus azarolus">Crataegus azarolus</a>, <a href="https://publications.waset.org/abstracts/search?q=polyphenols" title=" polyphenols"> polyphenols</a>, <a href="https://publications.waset.org/abstracts/search?q=flavonoids" title=" flavonoids"> flavonoids</a>, <a href="https://publications.waset.org/abstracts/search?q=hypertension" title=" hypertension"> hypertension</a>, <a href="https://publications.waset.org/abstracts/search?q=antioxidant%20activity" title=" antioxidant activity"> antioxidant activity</a>, <a href="https://publications.waset.org/abstracts/search?q=free%20radicals" title=" free radicals"> free radicals</a>, <a href="https://publications.waset.org/abstracts/search?q=peroxidation" title=" peroxidation"> peroxidation</a> </p> <a href="https://publications.waset.org/abstracts/10557/hypotensive-free-radical-scavenging-and-anti-lipid-peroxidation-activities-of-crataegus-azarolus-l-leaves-extracts-growing-in-algeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/10557.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">347</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Investigating Combined Effect of Aerobic Training and Crataegus elbursensis Extract on Plasma Angiogenic Mediators in Male Wistar Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Behnam%20Hasanzadeh">Behnam Hasanzadeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Asieh%20Abbassi%20Daloii"> Asieh Abbassi Daloii</a>, <a href="https://publications.waset.org/abstracts/search?q=Ailin%20Ebrahimi"> Ailin Ebrahimi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present study aims to investigate the effect of six weeks of progressive aerobic training and aqueous extract of Crataegus monogyna on VEGF variations and plasma angiopoetin-1/2 (ANG- 1/2) in male Wistar rats. Materials and methods: A number of 30 male Wistar rats, 4-6 months old, were randomly divided into four groups: control Crataegus monogyna (N=8), training Crataegus monogyna (N=8), control saline (N=6), and training saline (N=8). The aerobic training program included running on the treadmill at the speed of 34 meters per minute for 60 minutes per day. The training was conducted for six weeks, five days a week. Following each training session, both experimental and control subjects of Crataegus monogyna groups were orally fed with 0.5 mg Crataegus monogyna extract per gram of body weight. The normal saline group was given the same amount of normal saline solution (NS). Eventually, 72 hours after the last training session, blood samples were taken from inferior verna cava. Results: the results showed that progressive endurance training caused insignificant reductions in plasma VEGF concentration in the training group, Crataegus monogyna group and training-Crataegus monogyna group comparing the control group. Plasma ANG-1 concentration reduced in the training, Crataegus monogyna and training-Crataegus monogyna groups while plasma ANG-2 concentration significantly increased in the Crataegus monogyna group (P=0.022) comparing with the control group. The results showed that the ratio of plasma ANG-2 to ANG-1 significantly increased in the Crataegus monogyna group (P=0.028) comparing with the control group. Conclusion: the findings suggest that combination of aerobic training and Crataegus monogyna extract has no synergistic effect on anti-angiogenesis indicators and Crataegus monogyna extract had a stronger effect on anti-angiogenesis indicators. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=angiopoetin-1%2F2" title="angiopoetin-1/2">angiopoetin-1/2</a>, <a href="https://publications.waset.org/abstracts/search?q=VEGF" title=" VEGF"> VEGF</a>, <a href="https://publications.waset.org/abstracts/search?q=Crataegus%20monogyna%20%20extract" title=" Crataegus monogyna extract"> Crataegus monogyna extract</a>, <a href="https://publications.waset.org/abstracts/search?q=aerobic%20training" title=" aerobic training "> aerobic training </a> </p> <a href="https://publications.waset.org/abstracts/36020/investigating-combined-effect-of-aerobic-training-and-crataegus-elbursensis-extract-on-plasma-angiogenic-mediators-in-male-wistar-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/36020.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">432</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Heart and Plasma LDH and CK in Response to Intensive Treadmill Running and Aqueous Extraction of Red Crataegus pentagyna in Male Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Abdi">A. Abdi</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Barari"> A. Barari</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Hojatollah%20Nikbakht"> A. Hojatollah Nikbakht</a>, <a href="https://publications.waset.org/abstracts/search?q=Khosro%20Ebrahim"> Khosro Ebrahim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aim: The purpose of the current study was to investigate the effect of a high intensity treadmill running training (8 weeks) with or without aqueous extraction of Crataegus pentagyna on heart and plasma LDH and CK. Design: Thirty-two Wistar male rats (4-6 weeks old, 125-135 gr weight) were used. Animals were randomly assigned into training (n = 16) and control (n = 16) groups and further divided into saline-control (SC, n = 8), saline-training (ST, n = 8), red Crataegus pentagyna extraction -control (CPEC, n = 8), and red Crataegus pentagyna extraction -training (CPET, n = 8) groups. Training groups have performed a high-intensity running program 34 m/min on 0% grade, 60 min/day, 5 days/week) on a motor-driven treadmill for 8 weeks. Animals were fed orally with Crataegus extraction and saline solution (500mg/kg body weight/or 10ml/kg body weight) for last six weeks. Seventy- two hours after the last training session, rats were sacrificed; plasma and heart were excised and immediately frozen in liquid nitrogen. LDH and CK levels were measured by colorimetric method. Statistical analysis was performed using a one way analysis of variance and Tukey test. Significance was accepted at P = 0.05. Results: Result showed that consumption crataegus lowers LDH and CK in heart and plasma. Also the heart LDH and CK were lower in the CPET compared to the ST, while plasma LDH and CK in CPET was higher than the ST. The results of ANOVA showed that the due high-intensity exercise and consumption crataegus, there are significant differences between levels of hearth LDH (P < 0/001), plasma (P < 0/006) and hearth (P < 0/001) CK. Conclusion: It appears that high-intensity exercise led to increased tissue damage and inflammatory factors in plasma. In other hand, consumption aqueous extraction of Red Crataegus maybe inhibits these factors and prevents muscle and heart damage. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=LDH" title="LDH">LDH</a>, <a href="https://publications.waset.org/abstracts/search?q=CK" title=" CK"> CK</a>, <a href="https://publications.waset.org/abstracts/search?q=crataegus" title=" crataegus"> crataegus</a>, <a href="https://publications.waset.org/abstracts/search?q=intensity" title=" intensity"> intensity</a> </p> <a href="https://publications.waset.org/abstracts/35073/heart-and-plasma-ldh-and-ck-in-response-to-intensive-treadmill-running-and-aqueous-extraction-of-red-crataegus-pentagyna-in-male-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35073.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">437</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> The Effect of Aerobic Training and Aqueous Extract of C. monogyna (Hawthorn) on Plasma and Heart Angiogenic Mediators in Male Wistar Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Asieh%20Abbassi%20Daloii">Asieh Abbassi Daloii</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmad%20Abdi"> Ahmad Abdi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Sports information suggests that physical inactivity increases the risk of many diseases, including atherosclerosis. Coronary heart disease, stroke and peripheral vascular disease, atherosclerosis and clinical protests. However, exercise can have beneficial effects on risk factors for atherosclerosis by reducing hyperlipidemia, hypertension, obesity, plaque density, increased insulin sensitivity and glucose tolerance is improved. Despite these findings, there is little information about the molecular mechanisms of interaction between the body and its relation to sport and there arteriosclerosis. The present study aims to investigate the effect of six weeks of progressive aerobic training and aqueous extract of crataegus monogyna on vascular endothelial growth factor (VEGF) variations and angiopoetin-1/2 (ANG- 1/2) in plasma and heart tissue in male Wistar rats. Methods: 30 male Wistar rats, 4-6 months old, were randomly divided into four groups: control crataegus monogyna (N=8), training crataegus monogyna (N=8), control saline (N=6), and training saline (N=8). The aerobic training program included running on treadmill at the speed of 34 meters per minute for 60 minutes per day. The training was conducted for six weeks, five days a week. Following each training session, both experimental and control subjects of crataegus monogyna groups were orally fed with 0.5 mg crataegus monogyna extract per gram of the body weight. The normal saline group was given the same amount of the normal saline solution (NS). Eventually, 72 hours after the last training session, blood samples were taken from inferior Verna cava. Conclusion: It is likely that crataegus monogyna extract compared with aerobic training and even combination of both training and crataegus monogyna extract is more effective on angiogenesis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=angiopoietin%201" title="angiopoietin 1">angiopoietin 1</a>, <a href="https://publications.waset.org/abstracts/search?q=2" title="2">2</a>, <a href="https://publications.waset.org/abstracts/search?q=vascular%20endothelial%20growth%20factor" title=" vascular endothelial growth factor"> vascular endothelial growth factor</a>, <a href="https://publications.waset.org/abstracts/search?q=aerobic%20exercise" title=" aerobic exercise"> aerobic exercise</a> </p> <a href="https://publications.waset.org/abstracts/35351/the-effect-of-aerobic-training-and-aqueous-extract-of-c-monogyna-hawthorn-on-plasma-and-heart-angiogenic-mediators-in-male-wistar-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35351.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">385</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Plasma Collagen XVIII in Response to Intensive Aerobic Running and Aqueous Extraction of Black Crataegus Elbursensis in Male Rats</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Abdi">A. Abdi</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Abbasi%20Daloee"> A. Abbasi Daloee</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Barari"> A. Barari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Aim: The adaptations that occur in human body after doing exercises training are a factor to help healthy people stay away from certain diseases. One of the main adaptations is a change in blood circulation, especially in vessels. The increase of capillary density is dependent on the balance between angiogenic and angiostatic factors. Most studies show that the changes made to angiogenic developmental factors resulted from physical exercises indicate the low level of stimulators compared with inhibitors. It is believed that the plasma level of VEGF-A, the important angiogenic factor, is reduced after physical exercise. Findings indicate that the extract of crataegus plant reduces the platelet-derived growth factor receptor (PDGFR) autophosphorylation in human's fibroblast. More importantly, crataegus (1 to 100 mg in liter) clearly leads to the inhibition of PDGFR autophosphorylation in vascular smooth muscle cells (VSMCs). Angiogenesis is a process that can be classified into physiological and pathophysiological forms. collagen XVIII is a part of extracellular protein and heparan sulfate proteoglycans in vascular epithelial and endothelial basement membrane cause the release of endostatin from noncollagenous collagen XVIII. Endostatin inhibits the growth of endothelial cells, inhibits angiogenesis, weakens different types of cancer, and the growth of tumors. The purpose of the current study was to investigate the effect of intensive aerobic running with or without aqueous extraction of black Crataegus elbursensis on Collagen XVIII in male rats. Design: Thirty-two Wistar male rats (4-6 weeks old, 125-135 gr weight) were acquired from the Pasteur's Institute (Amol, Mazandaran), and randomly assigned into control (n = 16) and training (n = 16) groups. Rats were further divided into saline-control (SC) (n=8), saline-training (ST) (n=8), crataegus pentaegyna extraction -control (CPEC) (n=8), and crataegus pentaegyna extraction - training (CPET) (n=8). The control (SC and CPEC) groups remained sedentary; whereas the training groups underwent a high running exercise program. plasma were excised and immediately frozen in liquid nitrogen. Statistical analysis was performed using a one way analysis of variance and Tukey test. Significance was accepted at P = 0.05. Results: The results show that aerobic exercise group had the highest concentration collagen XVIII compared to other groups and then respectively black crataegus, training-crataegus and control groups. Conclusion: In general, researchers in this study concluded that the increase of collagen XVIII (albeit insignificant) as a result of physical activity and consumption of black crataegus extract could possibly serve as a regional inhibitor of angiogenesis and another evidence for the anti-cancer effects of physical activities. Since the research has not managed in this study to measure the amount of plasma endostatin, it is suggested that both indices are measured with important angiogenic factors so that we can have a more accurate interpretation of changes to angiogenic and angiostatic factors resulted from physical exercises. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=aerobic%20running" title="aerobic running">aerobic running</a>, <a href="https://publications.waset.org/abstracts/search?q=Crataegus%20elbursensis" title=" Crataegus elbursensis"> Crataegus elbursensis</a>, <a href="https://publications.waset.org/abstracts/search?q=Collagen%20XVIII" title=" Collagen XVIII "> Collagen XVIII </a> </p> <a href="https://publications.waset.org/abstracts/34936/plasma-collagen-xviii-in-response-to-intensive-aerobic-running-and-aqueous-extraction-of-black-crataegus-elbursensis-in-male-rats" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/34936.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">325</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Phytochemical Investigation and Diuretic Activity of the Palestinian Crataegus aronia in Mice Using an Aqueous Extract</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Belal%20Rahhal">Belal Rahhal</a>, <a href="https://publications.waset.org/abstracts/search?q=Isra%20Taha"> Isra Taha</a>, <a href="https://publications.waset.org/abstracts/search?q=Insaf%20Najajreh"> Insaf Najajreh</a>, <a href="https://publications.waset.org/abstracts/search?q=Waleed%20Basha"> Waleed Basha</a>, <a href="https://publications.waset.org/abstracts/search?q=Hamzeh%20Alzabadeh"> Hamzeh Alzabadeh</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahed%20Zyoud"> Ahed Zyoud</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Phytochemical Investigation and Diuretic Activity of the Palestinian Crataegus aronia in Mice using an Aqueous Extract Division of Physiology, Pharmacology and Toxicology Faculty of Medicine and Health Sciences An- Najah National University Nablus- Palestine Belal Rahhal, Isra Taha, Insaf Najajreh, Waleed Basha, Hamzeh Alzabadeh and Ahed Zyoud Purpose: Throughout history, various natural materials were used as remedies for treatment of various diseases, and recently a vastly growing and renewed interest in herbal medicine is witnessed globally. In Palestinian folk medicine, Crataegus aronia is used as a diuretic and for treatment of hypertension. This study aimed to assess the preliminary phytochemical properties and the diuretic effect of the aqueous extracts of this plant in mice after its intraperitonial administration. Methods: It is an experimental trial applied on mice (n=8, Male, CD-1, weight range: [25-30 gram]), which are divided into two groups (4 in each). The first group administered with the plant extract (500 mg/kg) , and the second with normal saline as negative control group. Then urine output and electrolyte contents were quantified up to 6 hours for the three groups and then compared to the control one. Results: Preliminary phytochemical screening reveals the presence of tannins, alkaloids and flavoniods as major phytoconstituents in aqueous extract. Significant diuresis was noted in those received the aqueous extract of Crataegus aronia (p < 0.05) compared to controls. Moreover, aqueous extract had an acidic pH and a mild increase in the electrolyte excretion (Na, K). Conclusions: Our results revealed that Crataegus aronia aqueous extract has a potential diuretic effect. Further studies are needed to evaluate this diuretic effect in the relief of diseases characterized by volume overload. Keywords: C. aronia, furosemide, diuresis, mice, medicinal plants. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=medicinal%20plants" title=" medicinal plants"> medicinal plants</a>, <a href="https://publications.waset.org/abstracts/search?q=diuretic%20activity" title=" diuretic activity"> diuretic activity</a>, <a href="https://publications.waset.org/abstracts/search?q=mice" title=" mice"> mice</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20aronia" title=" C. aronia"> C. aronia</a>, <a href="https://publications.waset.org/abstracts/search?q=" title=" "> </a>, <a href="https://publications.waset.org/abstracts/search?q=furosemide" title=" furosemide"> furosemide</a>, <a href="https://publications.waset.org/abstracts/search?q=" title=" "> </a>, <a href="https://publications.waset.org/abstracts/search?q=Phytochemical%20Investigation" title=" Phytochemical Investigation "> Phytochemical Investigation </a> </p> <a href="https://publications.waset.org/abstracts/123995/phytochemical-investigation-and-diuretic-activity-of-the-palestinian-crataegus-aronia-in-mice-using-an-aqueous-extract" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/123995.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">198</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Plant Cell Culture to Produce Valuable Natural Products</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jehad%20Dumireih">Jehad Dumireih</a>, <a href="https://publications.waset.org/abstracts/search?q=Malak%20Dmirieh"> Malak Dmirieh</a>, <a href="https://publications.waset.org/abstracts/search?q=Michael%20Wink"> Michael Wink</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The present work is aimed to use plant cell suspension cultures of Crataegus monogyna for biosynthesis of valuable natural products by using quercetin as an inexpensive precursor. Suspension cell cultures of C. monogyna were established by using Murashige and Skoog medium (MS) supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid and 1 mg/L kinetin. Cells were harvested from the cultures and extracted by using methanol and ethyl acetate; then the extracts were used for the identification of isoquercetin by HPLC and by mass spectrometry. The incubation of the cells with 0.24 mM quercetin for one week resulted in an 16 fold increase of isoquercetin biosynthesis; the growth rate of the cells increased by 20%. Moreover, the biosynthesis of isoquercetin was enhanced by 40% when we divided the added quercetin into three portions each one with concentration 0.12 mM supplied at 3 days intervals. In addition, we didn’t find any positive effects of adding different concentrations the precursors phenylalanine (0.2 mM) and galactose to the cell cultures. In conclusion, the efficiency of the biotransformation of quercetin into isoquercetin depended on the concentration quercetin, its incubation time and the way of its administration. The results of the present work suggest that the biotechnological methods such as cell suspension cultures could be successfully used to obtain highly valuable natural product starting from inexpensive compound. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biosynthesis" title="biosynthesis">biosynthesis</a>, <a href="https://publications.waset.org/abstracts/search?q=biotransformation" title=" biotransformation"> biotransformation</a>, <a href="https://publications.waset.org/abstracts/search?q=Crataegus" title=" Crataegus"> Crataegus</a>, <a href="https://publications.waset.org/abstracts/search?q=isoquercetin" title=" isoquercetin"> isoquercetin</a> </p> <a href="https://publications.waset.org/abstracts/34551/plant-cell-culture-to-produce-valuable-natural-products" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/34551.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">499</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> The Structure and Composition of Plant Communities in Ajluon Forest Reserve in Jordan</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Maher%20J.%20Tadros">Maher J. Tadros</a>, <a href="https://publications.waset.org/abstracts/search?q=Yaseen%20Ananbeh"> Yaseen Ananbeh</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The study area is located in Ajluon Forest Reserve northern part of Jordan. It consists of Mediterranean hills dominated by open woodlands of oak and pistachio. The aims of the study were to investigate the positive and negative relationships between the locals and the protected area and how it can affect the long-term forest conservation. The main research objectives are to review the impact of establishing Ajloun Forest Reserve on nature conservation and on the livelihood level of local communities around the reserve. The Ajloun forest reserve plays a fundamental role in Ajloun area development. The existence of initiatives of nature conservation in the area supports various socio-economic activities around the reserve that contribute towards the development of local communities in Ajloun area. A part of this research was to conduct a survey to study the impact of Ajloun forest reserve on biodiversity composition. Also, studying the biodiversity content especially for vegetation to determine the economic impacts of Ajloun forest reserve on its surroundings was studied. In this study, several methods were used to fill the objectives including point-centered quarter method which involves selecting randomly 50 plots at the study site. The collected data from the field showed that the absolute density was (1031.24 plant per hectare). Density was recorded and found to be the highest for Quecus coccifera, and relative density of (73.7%), this was followed by Arbutus andrachne and relative density (7.1%), Pistacia palaestina and relative density (10.5%) and Crataegus azarulus (82.5 p/ha) and relative density (5.1%), <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=composition" title="composition">composition</a>, <a href="https://publications.waset.org/abstracts/search?q=density" title=" density"> density</a>, <a href="https://publications.waset.org/abstracts/search?q=frequency" title=" frequency"> frequency</a>, <a href="https://publications.waset.org/abstracts/search?q=importance%20value" title=" importance value"> importance value</a>, <a href="https://publications.waset.org/abstracts/search?q=point-centered%20quarter" title=" point-centered quarter"> point-centered quarter</a>, <a href="https://publications.waset.org/abstracts/search?q=structure" title=" structure"> structure</a>, <a href="https://publications.waset.org/abstracts/search?q=tree%20cover" title=" tree cover"> tree cover</a> </p> <a href="https://publications.waset.org/abstracts/76363/the-structure-and-composition-of-plant-communities-in-ajluon-forest-reserve-in-jordan" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/76363.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">278</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Effects of Hawthorn (Crataegus monogyna) Polyphenols on Oxymyoglobin and Myofibrillar Proteins Stability in Meat</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Valentin%20Nicorescu">Valentin Nicorescu</a>, <a href="https://publications.waset.org/abstracts/search?q=Nicoleta%20C.%20Predescu"> Nicoleta C. Predescu</a>, <a href="https://publications.waset.org/abstracts/search?q=Camelia%20Papuc"> Camelia Papuc</a>, <a href="https://publications.waset.org/abstracts/search?q=Iuliana%20Gajaila"> Iuliana Gajaila</a>, <a href="https://publications.waset.org/abstracts/search?q=Carmen%20D.%20Petcu"> Carmen D. Petcu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The oxidation of the fresh muscle oxymyoglobin (bright red colour) to metmyoglobin (brown colour) leads to discoloration of red meats. After slaughter, enzymatic systems involved in metmyoglobin reduction are continually depleted as time post-mortem progresses, thus the meat colour is affected. Phenolic compounds are able to scavenge reactive species involved in oxymyoglobin oxidation and to reduce metmyoglobin to oxymyoglobin. The aim of this study was to investigate the effect of polyphenols extracted from hawthorn fruits on the stability of oxymyoglobin and myofibrillar proteins in ground pork subject to refrigeration for 6 days. Hawthorn polyphenols (HP) were added in ground pork in 100, 200 and 300 ppm concentrations. Oxymyoglobin and metmyoglobin were evaluated spectrophotometrically at every 2 days and electrophoretic pattern of myofibrillar proteins was investigated at days 0 and 6 by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). For all meat samples, oxymyoglobin concentration significantly decreased during the first 4 days of refrigeration. After 6 days, the significant decrease of oxymyoglobin concentration continued only in the negative control samples. In samples treated with HP and butylated hydroxylanisole (BHA - positive control), oxymyoglobin concentration increased after 6 days of refrigeration, the highest levels complying with the following order: 100 ppm HP > 200 ppm HP > 300 ppm HP > 100 ppm BHA. The increase in metmyoglobin was coincidental with the decrease in oxymyoglobin; metmyoglobin concentration progressively increased during the first 4 days of refrigeration in all meat samples. After 6 days, in meat samples treated with HP and BHA, lower metmyoglobin concentrations were found (compared to day 4), respecting the following order: 100 ppm HP < 200 ppm HP < 300 ppm HP < 100 ppm BHA. These results showed that hawthorn polyphenols and BHA reduced metmyoglobin (MbFe3+) to oxymyoglobin (MbFe2+), and the strongest reducing character was recorded for 100 ppm HP. After 6 days of refrigeration, electrophoretic pattern of myofibrillar proteins showed minor changes compared to day 0, indicating that HP prevent protein degradation as well as synthetic antioxidant BHA. Also, HP did not induce cross-links in the myofibrillar proteins, to form protein aggregates, and no risk of reducing their ability to retain water was identified. The pattern of oxymyoglobin and metmyoglobin concentrations determined in this study showed that hawthorn polyphenols are able to reduce metmyoglobin to oxymyoglobin and to delay oxymyoglobin oxidation, especially when they are added to ground meat in concentration of 100 ppm. This work was carried out through Partnerships in priority areas Program – PN II, implemented with the support of MEN – UEFISCDI (Romania), project nr. 149/2014. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hawthorn%20polyphenols" title="Hawthorn polyphenols">Hawthorn polyphenols</a>, <a href="https://publications.waset.org/abstracts/search?q=metmyoglobin" title=" metmyoglobin"> metmyoglobin</a>, <a href="https://publications.waset.org/abstracts/search?q=oxymyoglobin" title=" oxymyoglobin"> oxymyoglobin</a>, <a href="https://publications.waset.org/abstracts/search?q=proteins%20stability" title=" proteins stability"> proteins stability</a> </p> <a href="https://publications.waset.org/abstracts/48758/effects-of-hawthorn-crataegus-monogyna-polyphenols-on-oxymyoglobin-and-myofibrillar-proteins-stability-in-meat" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/48758.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">218</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Explore <li><a href="https://waset.org/disciplines">Disciplines</a></li> <li><a href="https://waset.org/conferences">Conferences</a></li> <li><a href="https://waset.org/conference-programs">Conference Program</a></li> <li><a href="https://waset.org/committees">Committees</a></li> <li><a href="https://publications.waset.org">Publications</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Research <li><a href="https://publications.waset.org/abstracts">Abstracts</a></li> <li><a href="https://publications.waset.org">Periodicals</a></li> <li><a href="https://publications.waset.org/archive">Archive</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Open Science <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Philosophy.pdf">Open Science Philosophy</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Science-Award.pdf">Open Science Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Open-Society-Open-Science-and-Open-Innovation.pdf">Open Innovation</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Postdoctoral-Fellowship-Award.pdf">Postdoctoral Fellowship Award</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/Scholarly-Research-Review.pdf">Scholarly Research Review</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Support <li><a href="https://waset.org/page/support">Support</a></li> <li><a href="https://waset.org/profile/messages/create">Contact Us</a></li> <li><a href="https://waset.org/profile/messages/create">Report Abuse</a></li> </ul> </div> </div> </div> </div> </div> <div class="container text-center"> <hr style="margin-top:0;margin-bottom:.3rem;"> <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank" class="text-muted small">Creative Commons Attribution 4.0 International License</a> <div id="copy" class="mt-2">&copy; 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