{"title":"Effect of Oral Administration of \u201cGadagi\u201c Tea on Liver Function in Rats","authors":"A. M. Gadanya, M. S. Sule, M. K. Atiku","volume":73,"journal":"International Journal of Bioengineering and Life Sciences","pagesStart":76,"pagesEnd":80,"ISSN":"1307-6892","URL":"https:\/\/publications.waset.org\/pdf\/10182","abstract":"Effect of oral administration of \u201cGadagi\" tea on liver\nfunction was assessed on 50 healthy male albino rats which were\ngrouped and administered with different doses(mg\/kg) i.e low dose\n(380mg\/kg, 415mg\/kg, 365mg\/kg, 315mg\/kg for \u201csak\", \u201csada\" and\n\u201cmagani\" respectively), standard dose ( 760mg\/kg, 830mg\/kg,\n730mg\/kg for \u201csak-, \u201csada\" and \u201cmagani\" respectively) and high dose\n(1500mg\/kg, 1700mg\/kg and 1460mg\/kg for \u201csak--,\"sada\" and\n\u201cmagani\" groups respectively) for a period of four weeks. Animals\nthat were not administered with the tea constituted the control group.\nAt the end of fourth week, the animals were sacrificed and their\nserum alanine aminotransferase (ALT), aspartate aminotransferase\n(AST), alkaline phosphatase (ALP), total protein (TP), albumin\n(ALB), and globulins (GLO) were determined. Mean serum ALT and\nALP activities were significantly higher (P<0.05) in rats orally\nadministered with high dose of \u201csak\" and those administered with\nstandard dose of \u201csada\" than those of the control group, suggesting a\nprobable impairment of liver function due to liver cytolysis.Mean\nserum AST, ALT and ALP activities were significantly lower\n(P<0.05) in rats that were orally administered with high dose of\n\u201cmagani\" than that of the control group, suggesting a probable\nimprovement in liver function (due to decrease in liver cytolysis).\nMean serum TP, ALB and GLO levels were significantly higher\n(P<0.05) in rats that were orally administered with the various doses\nof\u201csak\", \u201csada\" and \u201cmagani\" than those of the control group. This\nalso suggests a probable improvement in the synthetic function of the\nliver.Thus, some dosages of \u201csak\" and \u201csada could be hepatotoxic,\nwhereas \u201cmagani\" especially at the high dose administered could\nhave pharmacologically positive effect on the liver of the rats.","references":"[1] Kent, M., Vande, G. and Struart, I.F.(1999). Concepts of Human\nAnatomy and Physiology. Fifth edition. D.Van Nostrand Company, New\nYork. Pp 838-845.\n[2] Gadanya, A.M. (2011). Biochemical and Toxicological studies on\n\"Gadagi\"tea in rats. Ph.D. Thesis Department of Biochemistry, Bayero\nUniversity Kano, P2.\n[3] Reitman, S. and Frankel, S. (1957). Colorimetric method for the\ndetermination of serum glutamic oxaloacetate and glutamic pyruvic\ntransaminases. Amer. J. Clin. Pathol, 28; 56-61.\n[4] King, E.J. and Armstrong, A.R. (1964). Determination of serum alkaline\nphosphatase activity. Canadian Medical Journal 31:376-377.\n[5] Gornall, A.G., Bardawill, C.J. and David, M.M. (1949). Determination\nof serum proteins by means of buiret reaction. Journal of Biological\nChemistry. 28: 177 - 175.\n[6] Jandrassik, L. and Grof, P. (1938). Colorimetric Method for the\nDetermination Srum Bilirubin. Clinial Biochemistry.297:81.\n[7] Doumas, B.T., Waston, W.A. and Briggs, M.A. (1971). Albumin\nstandards and the measurement of serum albumin with bromocresol\ngreen. Clinical Chemical Acta, 31: 87-89.\n[8] Wada, H. and Snell, E.E. (1962). Enzymatic transmination of\npyridoxamine - pyruvate transaminase. J. Biol. Chem. 237: 133 - 137\n[9] Gadanya, A.M.,Sule, M.S. and Atiku, M.K. (2011). Analysis of some\nPhytochemicals in \"Gadagi\" tea commonly consumed in Kano,\nNigeria.(Unpublished manuscript).\n[10] Obi, F.O. and Uneh, E. (2003). pH department prevention of carbon\ntetrachloride-induced lipoperoxidation in rats by ethanolic extract of\nHibiscus rosasinensis petal. Biokeemstri 13:42-50.\n[11] Atiku, M.K., Adamu, D.J.M., Gadanya, A.M. and Shehu, M.A. (2009).\nThe effect of \"Gadagi\" tea on liver function and serum glucose\nconcentration in Albino rats. Bayero Journal of Pure and Applied\nSciences 2 (1):125-127.","publisher":"World Academy of Science, Engineering and Technology","index":"Open Science Index 73, 2013"}