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li{display:table-cell;float:none!important;width:10%;padding:0 15px;border-right:1px solid rgba(0,0,0,0.05)}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li.hide-border{border-right-width:0}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li a{border-bottom-width:0;padding:9px 15px;line-height:120%}.mm-vertical #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{font-weight:700}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:first-child{border-right-width:0}.rtl .mm-vertical #Top_bar .menu > li > ul.mfn-megamenu > li:last-child{border-right-width:1px}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:980px!important;margin:0}.header-plain:not(.menuo-right) #Header .top_bar_left{width:auto!important}.header-stack.header-center #Top_bar #menu{display:inline-block!important}.header-simple #Top_bar #menu{display:none;height:auto;width:300px;bottom:auto;top:100%;right:1px;position:absolute;margin:0}.header-simple #Header a.responsive-menu-toggle{display:block;right:10px}.header-simple #Top_bar #menu > ul{width:100%;float:left}.header-simple #Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}.header-simple #Top_bar #menu ul li a{padding:0 20px;margin:0;display:block;height:auto;line-height:normal;border:none}.header-simple #Top_bar #menu ul li a:after{display:none}.header-simple #Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}.header-simple #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}.header-simple #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}.header-simple #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}.header-simple #Top_bar #menu ul li.hover a{border-bottom:0}.header-simple #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}.header-simple #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin:0!important;width:auto!important;background-image:none}.header-simple #Top_bar #menu ul li ul li{width:100%!important;display:block;padding:0}.header-simple #Top_bar #menu ul li ul li a{padding:0 20px 0 30px}.header-simple #Top_bar #menu ul li ul li a .menu-arrow{display:none}.header-simple #Top_bar #menu ul li ul li a span{padding:0}.header-simple #Top_bar #menu ul li ul li a span:after{display:none!important}.header-simple #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}.header-simple #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}.header-simple #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 20px 0 40px}.rtl.header-simple #Top_bar #menu{left:1px;right:auto}.rtl.header-simple #Top_bar a.responsive-menu-toggle{left:10px;right:auto}.rtl.header-simple #Top_bar #menu ul li.submenu .menu-toggle{left:0;right:auto}.rtl.header-simple #Top_bar #menu ul li ul{left:auto!important;right:0!important}.rtl.header-simple #Top_bar #menu ul li ul li a{padding:0 30px 0 20px}.rtl.header-simple #Top_bar #menu ul li ul li ul li a{padding:0 40px 0 20px}.menu-highlight #Top_bar .menu > li{margin:0 2px}.menu-highlight:not(.header-creative) #Top_bar .menu > li > a{margin:20px 0;padding:0;-webkit-border-radius:5px;border-radius:5px}.menu-highlight #Top_bar .menu > li > a:after{display:none}.menu-highlight #Top_bar .menu > li > a span:not(.description){line-height:50px}.menu-highlight #Top_bar .menu > li > a span.description{display:none}.menu-highlight.header-stack #Top_bar .menu > li > a{margin:10px 0!important}.menu-highlight.header-stack #Top_bar .menu > li > a span:not(.description){line-height:40px}.menu-highlight.header-transparent #Top_bar .menu > li > a{margin:5px 0}.menu-highlight.header-simple #Top_bar #menu ul li,.menu-highlight.header-creative #Top_bar #menu ul li{margin:0}.menu-highlight.header-simple #Top_bar #menu ul li > a,.menu-highlight.header-creative #Top_bar #menu ul li > a{-webkit-border-radius:0;border-radius:0}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a{margin:10px 0!important;padding:5px 0!important}.menu-highlight:not(.header-fixed):not(.header-simple) #Top_bar.is-sticky .menu > li > a span{line-height:30px!important}.header-modern.menu-highlight.menuo-right .menu_wrapper{margin-right:20px}.menu-line-below #Top_bar .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}.menu-line-below-80 #Top_bar:not(.is-sticky) .menu > li > a:after{height:4px;left:10%;top:50%;margin-top:20px;width:80%}.menu-line-below-80-1 #Top_bar:not(.is-sticky) .menu > li > a:after{height:1px;left:10%;top:50%;margin-top:20px;width:80%}.menu-link-color #Top_bar .menu > li > a:after{display:none!important}.menu-arrow-top #Top_bar .menu > li > a:after{background:none repeat scroll 0 0 rgba(0,0,0,0)!important;border-color:#ccc transparent transparent;border-style:solid;border-width:7px 7px 0;display:block;height:0;left:50%;margin-left:-7px;top:0!important;width:0}.menu-arrow-top.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-top.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-top #Top_bar.is-sticky .menu > li > a:after{top:0!important}.menu-arrow-bottom #Top_bar .menu > li > a:after{background:none!important;border-color:transparent transparent #ccc;border-style:solid;border-width:0 7px 7px;display:block;height:0;left:50%;margin-left:-7px;top:auto;bottom:0;width:0}.menu-arrow-bottom.header-transparent #Top_bar .menu > li > a:after,.menu-arrow-bottom.header-plain #Top_bar .menu > li > a:after{display:none}.menu-arrow-bottom #Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:0}.menuo-no-borders #Top_bar .menu > li > a span:not(.description){border-right-width:0}.menuo-no-borders #Header_creative #Top_bar .menu > li > a span{border-bottom-width:0}body.header-creative{padding-left:50px}body.header-creative.header-open{padding-left:250px}body.error404,body.under-construction,body.template-blank{padding-left:0!important}.header-creative.footer-fixed #Footer,.header-creative.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{box-sizing:border-box;padding-left:50px}.header-open.footer-fixed #Footer,.header-open.footer-sliding #Footer,.header-creative.footer-stick #Footer.is-sticky{padding-left:250px}.header-rtl.header-creative.footer-fixed #Footer,.header-rtl.header-creative.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-left:0;padding-right:50px}.header-rtl.header-open.footer-fixed #Footer,.header-rtl.header-open.footer-sliding #Footer,.header-rtl.header-creative.footer-stick #Footer.is-sticky{padding-right:250px}#Header_creative{background:#fff;position:fixed;width:250px;height:100%;left:-200px;top:0;z-index:9002;-webkit-box-shadow:2px 0 4px 2px rgba(0,0,0,.15);box-shadow:2px 0 4px 2px rgba(0,0,0,.15)}#Header_creative .container{width:100%}#Header_creative .creative-wrapper{opacity:0;margin-right:50px}#Header_creative a.creative-menu-toggle{display:block;width:34px;height:34px;line-height:34px;font-size:22px;text-align:center;position:absolute;top:10px;right:8px;border-radius:3px}.admin-bar #Header_creative a.creative-menu-toggle{top:42px}#Header_creative #Top_bar{position:static;width:100%}#Header_creative #Top_bar .top_bar_left{width:100%!important;float:none}#Header_creative #Top_bar .top_bar_right{width:100%!important;float:none;height:auto;margin-bottom:35px;text-align:center;padding:0 20px;top:0;-webkit-box-sizing:border-box;-moz-box-sizing:border-box;box-sizing:border-box}#Header_creative #Top_bar .top_bar_right:before{display:none}#Header_creative #Top_bar .top_bar_right_wrapper{top:0}#Header_creative #Top_bar .logo{float:none;text-align:center;margin:15px 0}#Header_creative #Top_bar .menu_wrapper{float:none;margin:0 0 30px}#Header_creative #Top_bar .menu > li{width:100%;float:none;position:relative}#Header_creative #Top_bar .menu > li > a{padding:0;text-align:center}#Header_creative #Top_bar .menu > li > a:after{display:none}#Header_creative #Top_bar .menu > li > a span{border-right:0;border-bottom-width:1px;line-height:38px}#Header_creative #Top_bar .menu li ul{left:100%;right:auto;top:0;box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03);-webkit-box-shadow:2px 2px 2px 0 rgba(0,0,0,0.03)}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu{width:700px!important}#Header_creative #Top_bar .menu > li > ul.mfn-megamenu > li > ul{left:0}#Header_creative #Top_bar .menu li ul li a{padding-top:9px;padding-bottom:8px}#Header_creative #Top_bar .menu li ul li ul{top:0!important}#Header_creative #Top_bar .menu > li > a span.description{display:block;font-size:13px;line-height:28px!important;clear:both}#Header_creative #Top_bar .search_wrapper{left:100%;top:auto;bottom:0}#Header_creative #Top_bar a#header_cart{display:inline-block;float:none;top:3px}#Header_creative #Top_bar a#search_button{display:inline-block;float:none;top:3px}#Header_creative #Top_bar .wpml-languages{display:inline-block;float:none;top:0}#Header_creative #Top_bar .wpml-languages.enabled:hover a.active{padding-bottom:9px}#Header_creative #Top_bar a.button.action_button{display:inline-block;float:none;top:16px;margin:0}#Header_creative #Top_bar .banner_wrapper{display:block;text-align:center}#Header_creative #Top_bar .banner_wrapper img{max-width:100%;height:auto;display:inline-block}#Header_creative #Action_bar{display:none;position:absolute;bottom:0;top:auto;clear:both;padding:0 20px;box-sizing:border-box}#Header_creative #Action_bar .social{float:none;text-align:center;padding:5px 0 15px}#Header_creative #Action_bar .social li{margin-bottom:2px}#Header_creative .social li a{color:rgba(0,0,0,.5)}#Header_creative .social li a:hover{color:#000}#Header_creative .creative-social{position:absolute;bottom:10px;right:0;width:50px}#Header_creative .creative-social li{display:block;float:none;width:100%;text-align:center;margin-bottom:5px}.header-creative .fixed-nav.fixed-nav-prev{margin-left:50px}.header-creative.header-open .fixed-nav.fixed-nav-prev{margin-left:250px}.menuo-last #Header_creative #Top_bar .menu li.last ul{top:auto;bottom:0}.header-open #Header_creative{left:0}.header-open #Header_creative .creative-wrapper{opacity:1;margin:0!important}.header-open #Header_creative .creative-menu-toggle,.header-open #Header_creative .creative-social{display:none}body.header-rtl.header-creative{padding-left:0;padding-right:50px}.header-rtl #Header_creative{left:auto;right:-200px}.header-rtl.nice-scroll #Header_creative{margin-right:10px}.header-rtl #Header_creative .creative-wrapper{margin-left:50px;margin-right:0}.header-rtl #Header_creative a.creative-menu-toggle{left:8px;right:auto}.header-rtl #Header_creative .creative-social{left:0;right:auto}.header-rtl #Footer #back_to_top.sticky{right:125px}.header-rtl #popup_contact{right:70px}.header-rtl #Header_creative #Top_bar .menu li ul{left:auto;right:100%}.header-rtl #Header_creative #Top_bar .search_wrapper{left:auto;right:100%}.header-rtl .fixed-nav.fixed-nav-prev{margin-left:0!important}.header-rtl .fixed-nav.fixed-nav-next{margin-right:50px}body.header-rtl.header-creative.header-open{padding-left:0;padding-right:250px!important}.header-rtl.header-open #Header_creative{left:auto;right:0}.header-rtl.header-open #Footer #back_to_top.sticky{right:325px}.header-rtl.header-open #popup_contact{right:270px}.header-rtl.header-open .fixed-nav.fixed-nav-next{margin-right:250px}#Header_creative.active{left:-1px}.header-rtl #Header_creative.active{left:auto;right:-1px}#Header_creative.active .creative-wrapper{opacity:1;margin:0}.header-creative .vc_row[data-vc-full-width]{padding-left:50px}.header-creative.header-open .vc_row[data-vc-full-width]{padding-left:250px}.header-open .vc_parallax .vc_parallax-inner{left:auto;width:calc(100% - 250px)}.header-open.header-rtl .vc_parallax .vc_parallax-inner{left:0;right:auto}#Header_creative.scroll{height:100%;overflow-y:auto}#Header_creative.scroll:not(.dropdown) .menu li ul{display:none!important}#Header_creative.scroll #Action_bar{position:static}#Header_creative.dropdown{outline:none}#Header_creative.dropdown #Top_bar .menu_wrapper{float:left}#Header_creative.dropdown #Top_bar #menu ul li{position:relative;float:left}#Header_creative.dropdown #Top_bar #menu ul li a:after{display:none}#Header_creative.dropdown #Top_bar #menu ul li a span{line-height:38px;padding:0}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:0;top:0;width:38px;height:38px;line-height:38px;font-size:26px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Header_creative.dropdown #Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Header_creative.dropdown #Top_bar #menu ul li.hover > .menu-toggle:after{content:"-"}#Header_creative.dropdown #Top_bar #menu ul li.hover a{border-bottom:0}#Header_creative.dropdown #Top_bar #menu ul.mfn-megamenu li .menu-toggle{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul{position:relative!important;left:0!important;top:0;padding:0;margin-left:0!important;width:auto!important;background-image:none}#Header_creative.dropdown #Top_bar #menu ul li ul li{width:100%!important}#Header_creative.dropdown #Top_bar #menu ul li ul li a{padding:0 10px;text-align:center}#Header_creative.dropdown #Top_bar #menu ul li ul li a .menu-arrow{display:none}#Header_creative.dropdown #Top_bar #menu ul li ul li a span{padding:0}#Header_creative.dropdown #Top_bar #menu ul li ul li a span:after{display:none!important}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu a.mfn-megamenu-title{text-transform:uppercase;font-weight:400}#Header_creative.dropdown #Top_bar .menu > li > ul.mfn-megamenu > li > ul{display:block!important;position:inherit;left:auto;top:auto}#Header_creative.dropdown #Top_bar #menu ul li ul li ul{border-left:0!important;padding:0;top:0}#Header_creative{transition:left .5s ease-in-out,right .5s ease-in-out}#Header_creative .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out .5s}#Header_creative.active .creative-wrapper{transition:opacity .5s ease-in-out,margin 0s ease-in-out}}@media only screen and (min-width:1130px){#Top_bar.is-sticky{position:fixed!important;width:100%;left:0;top:-60px;height:60px;z-index:701;background:#fff;opacity:.97;filter:alpha(opacity = 97);-webkit-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);-moz-box-shadow:0 2px 5px 0 rgba(0,0,0,0.1);box-shadow:0 2px 5px 0 rgba(0,0,0,0.1)}.layout-boxed.header-boxed #Top_bar.is-sticky{max-width:1130px;left:50%;-webkit-transform:translateX(-50%);transform:translateX(-50%)}.layout-boxed.header-boxed.nice-scroll #Top_bar.is-sticky{margin-left:-5px}#Top_bar.is-sticky .top_bar_left,#Top_bar.is-sticky .top_bar_right,#Top_bar.is-sticky .top_bar_right:before{background:none}#Top_bar.is-sticky .top_bar_right{top:-4px;height:auto}#Top_bar.is-sticky .top_bar_right_wrapper{top:15px}.header-plain #Top_bar.is-sticky .top_bar_right_wrapper{top:0}#Top_bar.is-sticky .logo{width:auto;margin:0 30px 0 20px;padding:0}#Top_bar.is-sticky #logo{padding:5px 0!important;height:50px!important;line-height:50px!important}.logo-no-sticky-padding #Top_bar.is-sticky #logo{height:60px!important;line-height:60px!important}#Top_bar.is-sticky #logo img.logo-main{display:none}#Top_bar.is-sticky #logo img.logo-sticky{display:inline;max-height:35px}#Top_bar.is-sticky .menu_wrapper{clear:none}#Top_bar.is-sticky .menu_wrapper .menu > li > a{padding:15px 0}#Top_bar.is-sticky .menu > li > a,#Top_bar.is-sticky .menu > li > a span{line-height:30px}#Top_bar.is-sticky .menu > li > a:after{top:auto;bottom:-4px}#Top_bar.is-sticky .menu > li > a span.description{display:none}#Top_bar.is-sticky .secondary_menu_wrapper,#Top_bar.is-sticky .banner_wrapper{display:none}.header-overlay #Top_bar.is-sticky{display:none}.sticky-dark #Top_bar.is-sticky{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky #menu{background:rgba(0,0,0,.8)}.sticky-dark #Top_bar.is-sticky .menu > li > a{color:#fff}.sticky-dark #Top_bar.is-sticky .top_bar_right a{color:rgba(255,255,255,.5)}.sticky-dark #Top_bar.is-sticky .wpml-languages a.active,.sticky-dark #Top_bar.is-sticky .wpml-languages ul.wpml-lang-dropdown{background:rgba(0,0,0,0.3);border-color:rgba(0,0,0,0.1)}}@media only screen and (min-width:768px) and (max-width:1130px){.header_placeholder{height:0!important}}@media only screen and (max-width:1129px){#Top_bar #menu{display:none;height:auto;width:100%;bottom:auto;top:100%;right:1px;position:absolute;margin:0}#Top_bar a.responsive-menu-toggle{display:block}#Top_bar #menu > ul{width:100%;float:left}#Top_bar #menu ul li{width:100%;padding-bottom:0;border-right:0;position:relative}#Top_bar #menu ul li a{padding:0 25px;margin:0;display:block;height:auto;line-height:normal;border:none}#Top_bar #menu ul li a:after{display:none}#Top_bar #menu ul li a span{border:none;line-height:44px;display:inline;padding:0}#Top_bar #menu ul li a span.description{margin:0 0 0 5px}#Top_bar #menu ul li.submenu .menu-toggle{display:block;position:absolute;right:15px;top:0;width:44px;height:44px;line-height:44px;font-size:30px;font-weight:300;text-align:center;cursor:pointer;color:#444;opacity:0.33}#Top_bar #menu ul li.submenu .menu-toggle:after{content:"+"}#Top_bar #menu ul li.hover > 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ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/high-throughput-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">High-throughput Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26914 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26915" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26915 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220-plus-focused-ultrasonicator-500569"><span class="ubermenu-target-title ubermenu-target-text">LE220-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High-throughput, 1 to 96 tube capacity</span></a></li><li id="menu-item-26916" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26916 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/le220r-plus-focused-ultrasonicator-500578"><span class="ubermenu-target-title ubermenu-target-text">LE220R-plus</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Robotic version of the LE220-plus</span></a></li><li id="menu-item-30065" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30065 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/le220rsc-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">LE220Rsc</span></a></li></ul></li><li id="menu-item-26917" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26917 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/on-deck-automation-focused-ultrasonicators"><span class="ubermenu-target-title ubermenu-target-text">On-Deck Automation Focused-ultrasonicators</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26917 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26918" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26918 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/r230-focused-ultrasonicator"><span class="ubermenu-target-title ubermenu-target-text">R230</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">On-deck, next generation Focused-ultrasonicator</span></a></li></ul></li><li id="menu-item-26919" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26919 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/cryoprep"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP<sup>®</sup> Extraction Systems</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26919 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26920" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26920 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp01-cryoprep-manual-dry-pulverizer-500230"><span class="ubermenu-target-title ubermenu-target-text">CP01 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 15 mg to 2 g</span></a></li><li id="menu-item-26921" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26921 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp02-cryoprep-automated-dry-pulverizer-110v-500001"><span class="ubermenu-target-title ubermenu-target-text">CP02 Automated Dry Pulverizer</span></a></li><li id="menu-item-26922" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26922 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-3" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/cp03-cryoprep-manual-dry-pulverizer-500049"><span class="ubermenu-target-title ubermenu-target-text">CP03 Manual Pulverizer</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">For Tissues 2 to 5 g</span></a></li></ul></li><li id="menu-item-26923" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26923 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/instruments/sonolab-software"><span class="ubermenu-target-title ubermenu-target-text">SonoLab Software</span></a></li></ul></li><li id="menu-item-26927" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26927 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents"><span class="ubermenu-target-title ubermenu-target-text">Kits & Reagents</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26927 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-41083" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-41083 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truprep-protein/"><span class="ubermenu-target-title ubermenu-target-text">truPREP® Protein Kits</span></a></li><li id="menu-item-35108" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-35108 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/truxtrac-ffpe-na-auto-96-kits"><span class="ubermenu-target-title ubermenu-target-text">truXTRAC® FFPE SMART Solutions</span></a></li><li id="menu-item-26931" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26931 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/cryoprep-0"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Starter Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cryoPREP Sample Tubes & Holders</span></a></li><li id="menu-item-26933" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26933 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/nucleic-acids-shearing-kits-and-buffers"><span class="ubermenu-target-title ubermenu-target-text">Nucleic Acids Shearing Kits and Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">truSHEAR, truPOP, DNA Verification Kits</span></a></li><li id="menu-item-26929" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26929 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-ffpe-kits"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> FFPE Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">FFPE Biomolecule Extraction Kits</span></a></li><li id="menu-item-26934" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26934 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truxtrac-other"><span class="ubermenu-target-title ubermenu-target-text">Other truXTRAC<sup>®</sup> Kits</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">cfDNA, DBS, MALDI-TOF</span></a></li><li id="menu-item-26935" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26935 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/protein-extraction-buffers"><span class="ubermenu-target-title ubermenu-target-text">Protein Extraction Buffers</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Native, Total Protein, & Detergent-free Buffers</span></a></li><li id="menu-item-26930" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26930 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/kits-reagents/truchip"><span class="ubermenu-target-title ubermenu-target-text">truChIP<sup>®</sup></span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Chromatin Shearing Kits</span></a></li></ul></li><li id="menu-item-26936" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26936 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables"><span class="ubermenu-target-title ubermenu-target-text">Consumables – Tubes and Vials</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26936 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26938" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26938 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/afa-tube"><span class="ubermenu-target-title ubermenu-target-text">AFA-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Laser etched polymer vessel for high-throughput workflows</span></a></li><li id="menu-item-26939" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26939 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/microtube"><span class="ubermenu-target-title ubermenu-target-text">microTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Small sample volumes, 130 µl or less</span></a></li><li id="menu-item-26940" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26940 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/millitube"><span class="ubermenu-target-title ubermenu-target-text">milliTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">1 ml or 2 ml sample volume. Good for chromatin shearing</span></a></li><li id="menu-item-26942" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26942 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/other-afa-tubes"><span class="ubermenu-target-title ubermenu-target-text">Other AFA-TUBEs</span></a></li><li id="menu-item-26943" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26943 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/afa-process-vessels/"><span class="ubermenu-target-title ubermenu-target-text">AFA Process Vessels</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">High power, low temperature processing</span></a></li><li id="menu-item-26944" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26944 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/consumables/g-tube"><span class="ubermenu-target-title ubermenu-target-text">g-TUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">DNA shearing fragments 6 to 20 kb</span></a></li></ul></li><li id="menu-item-26945" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26945 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables"><span class="ubermenu-target-title ubermenu-target-text">cryoPREP Consumables</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26945 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26947" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26947 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/cryoprep-consumables/tissuetube"><span class="ubermenu-target-title ubermenu-target-text">tissueTUBE</span><span class="ubermenu-target-divider"> – </span><span class="ubermenu-target-description ubermenu-target-text">Tissue processing devices</span></a></li></ul></li><li id="menu-item-26949" class="scroll-menu-top ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-has-children ubermenu-item-26949 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-1 ubermenu-has-submenu-drop ubermenu-has-submenu-flyout" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories"><span class="ubermenu-target-title ubermenu-target-text">Accessories</span><i class='ubermenu-sub-indicator fas fa-angle-down'></i></a><ul class="ubermenu-submenu ubermenu-submenu-id-26949 ubermenu-submenu-type-flyout ubermenu-submenu-drop ubermenu-submenu-align-left_edge_item ubermenu-submenu-retractor-top" ><li class="ubermenu-retractor ubermenu-retractor-mobile"><i class="fas fa-times"></i> Close</li><li id="menu-item-26952" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26952 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/automation-accessories"><span class="ubermenu-target-title ubermenu-target-text">Automation Accessories</span></a></li><li id="menu-item-41270" class="ubermenu-item ubermenu-item-type-post_type ubermenu-item-object-page ubermenu-item-41270 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="https://www.covaris.com/r230-accessories"><span class="ubermenu-target-title ubermenu-target-text">R230 Accessories</span></a></li><li id="menu-item-26953" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26953 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/m220-accessories"><span class="ubermenu-target-title ubermenu-target-text">M220 Accessories</span></a></li><li id="menu-item-26954" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26954 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/me220-accessories"><span class="ubermenu-target-title ubermenu-target-text">ME220 Accessories</span></a></li><li id="menu-item-30718" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-30718 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/ml230-accessories"><span class="ubermenu-target-title ubermenu-target-text">ML230 Accessories</span></a></li><li id="menu-item-26955" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26955 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/s220-accessories"><span class="ubermenu-target-title ubermenu-target-text">S220 Accessories</span></a></li><li id="menu-item-26956" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26956 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-evolution-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220<i>evolution</i> Accessories</span></a></li><li id="menu-item-26957" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26957 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/e220-accessories"><span class="ubermenu-target-title ubermenu-target-text">E220 Accessories</span></a></li><li id="menu-item-26958" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26958 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/l-series-accessories"><span class="ubermenu-target-title ubermenu-target-text">L-Series Accessories</span></a></li><li id="menu-item-26959" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26959 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp01-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP01 Accessories</span></a></li><li id="menu-item-26960" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26960 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp02-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP02 Accessories</span></a></li><li id="menu-item-26961" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26961 ubermenu-item-auto ubermenu-item-normal ubermenu-item-level-2" ><a class="ubermenu-target ubermenu-item-layout-default ubermenu-item-layout-text_only" href="/products-services/accessories/cp03-accessories"><span class="ubermenu-target-title ubermenu-target-text">CP03 Accessories</span></a></li><li id="menu-item-26963" class="ubermenu-item ubermenu-item-type-custom ubermenu-item-object-custom ubermenu-item-26963 ubermenu-item-auto 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!important;}.vc_custom_1682607466178{margin-bottom: 0px !important;}.vc_custom_1682607480930{margin-bottom: 0px !important;}.vc_custom_1682607567313{margin-top: 13px !important;margin-bottom: 0px !important;}</style> <div id="Content" class="cms_mage_page" style="padding: 0px;"> <div class="content_wrapper clearfix"> <div class="sections_group"> <div class="entry-content" itemprop="mainContentOfPage"> <div data-vc-full-width="true" data-vc-full-width-init="false" class="vc_row wpb_row vc_row-fluid shearing_banner vc_custom_1583475962885 vc_row-has-fill"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner vc_custom_1583475591897"><div class="wpb_wrapper"><h1 style="color: #ffffff;text-align: center" class="vc_custom_heading vc_do_custom_heading vc_custom_1646290188033" >Chromatin Fragmentation and Shearing</h1><h3 style="color: #ffffff;text-align: center" class="vc_custom_heading vc_do_custom_heading" >Covaris workflows enable standardized chromatin shearing from versatile samples such as primary cells, cultured cells, tissues, and FFPE samples. Covaris Adaptive Focused Acoustics® (AFA®) technology guarantees efficient and random chromatin fragmentation and supports nuclei extraction from hard to lyse specimens.</h3></div></div></div></div><div class="vc_row-full-width vc_clearfix"></div><div class="vc_row wpb_row vc_row-fluid cmspage_menu vc_custom_1583489874470 vc_column-gap-20"><div class="common-pages-left-container PrdMenu clearfix wpb_column vc_column_container vc_col-sm-3 vc_col-has-fill"><div class="vc_column-inner vc_custom_1583479740629"><div class="wpb_wrapper"><h4 style="color: #ffffff;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h4 vc_custom_1583481599852" >Contents</h4> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <ul id="shearing-sidebar"> <li class="active"><a style="cursor: pointer; display: block; text-decoration: none;" href="#overview">Overview</a></li> <li><a style="cursor: pointer; display: block; text-decoration: none;" href="#downstream">Downstream Epigenomic Applications: Translational Discoveries Powered by AFA-energetics®<br /> </a></li> <li class=""><a style="cursor: pointer; display: block; text-decoration: none;" href="#featured_product">Featured Products</a></li> <li class=""><a style="cursor: pointer; display: grid; text-decoration: none;" href="#resources">Resources</a></li> <li class=""><a style="cursor: pointer; display: grid; text-decoration: none;" href="#references">References</a></li> </ul> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner vc_custom_1580898106712"><div class="wpb_wrapper"><div id="overview" class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1583915637560"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <p><a class="image-popup-no-margins" href="https://www.covaris.com/wp/wp-content/uploads/2020/03/EpigenomicsWorkflow_v3.jpg"><br /> <img class="alignnone" src="https://www.covaris.com/wp/wp-content/uploads/2020/03/EpigenomicsWorkflow_v3.jpg" alt="Chromatin Shearing Process | standardization and flexibility " width="700" height="424" /><br /> </a></p> </div> </div> <h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Covaris truChIP®: A Different Approach to Chromatin Shearing</h2> <div class="wpb_text_column wpb_content_element vc_custom_1729078392965 instrument_bullet_list" > <div class="wpb_wrapper"> <h5 class="custom_h5">Epigenetic characterization provides a powerful tool to better understand cell-fate changes during development and disease. However, standardizing the sample preparation steps in chromatin assays is crucial to retrieve meaningful genome-wide epigenomic data that can be compared against different experimental set-ups and different laboratories.</h5> <h5 class="custom_h5">Covaris technology has enabled the standardization of chromatin preparation and shearing from virtually any sample input with consistent results. AFA technology in combination with optimized reagents provide researchers an easy, fast, reproducible and scalable workflow to retrieve high-quality sheared chromatin to analyze the epigenetic make-up of their sample on genome-wide scale.</h5> <h5 class="custom_h5">Chromatin shearing with truChIP and AFA has many benefits including:</h5> <ul> <li>Lower cell number requirement <ul> <li>Reduced cell culturing, enable analysis of scarce clinical samples</li> </ul> </li> <li>Controlled shearing size range <ul> <li>Reproducible ChIP-seq results</li> </ul> </li> <li>Isothermal processing <ul> <li>Maintaining epitope integrity and no reversal of crosslinks during shearing</li> </ul> </li> <li>Low detergent requirement (reduced SDS 0.1%) <ul> <li>Suitable for all IP protocols and less chromatin dilutions</li> </ul> </li> <li>Gentle shearing <ul> <li>Lower fixation time to avoid epitope masking</li> </ul> </li> <li>Universal protocol guaranteed to work with all mammalian cells</li> </ul> <p><a class="image-popup-no-margins" href="https://www.covaris.com/wp/wp-content/uploads/2020/04/StandardizedSampleChIP.jpg"><br /> </a><a class="image-popup-no-margins" href="https://www.covaris.com/wp/wp-content/uploads/2020/04/StandardizedSampleChIP.jpg"><img class="alignnone wp-image-34302 size-large" src="https://www.covaris.com/wp/wp-content/uploads/StandardizedSampleChIP-1024x461.jpg" alt="Chromatin Fragmentation key steps and benefits of Covaris truChIP" width="1024" height="461" /></a></p> <h5 class="custom_h5">Watch the video case study, Standardization of the ChIP Workflow Using Covaris Adaptive Focused Acoustics (AFA) to Study the Transcriptional Regulation of SOD1 in ALS Pathogenesis, presented by Byung Woo Kim, PhD Candidate, Johns Hopkins University and Hamid Khoja, PhD, Covaris</h5> </div> </div> <div class="wpb_video_widget wpb_content_element vc_clearfix vc_custom_1583915550359 vc_video-aspect-ratio-169 vc_video-el-width-100 vc_video-align-center" > <div class="wpb_wrapper"> <div class="wpb_video_wrapper"><iframe title="Standardization of the ChIP Workflow Using Covaris Adaptive Focused Acoustics (AFA)" width="500" height="281" src="https://www.youtube.com/embed/0uj7OhYO1wQ?feature=oembed" frameborder="0" allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share" referrerpolicy="strict-origin-when-cross-origin" allowfullscreen></iframe></div> </div> </div> <div class="wpb_text_column wpb_content_element" > <div class="wpb_wrapper"> <h5 style="text-align: center;">Download the Chromatin Preparation and Shearing Brochure, a presentation of Chromatin Applications enabled by AFA-energetics<sup>®</sup></h5> </div> </div> <div class="vc_btn3-container category_accordion vc_btn3-center vc_custom_1625658359839 vc_do_btn" ><a style="background-color:#ffffff; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom" href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020139.pdf" title="Chromatin Brochure" target="_blank">DOWNLOAD</a></div></div></div></div></div><div id="downstream" class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1583739502615"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Downstream Epigenomic Applications: Translational Discoveries Powered by AFA-energetics®</h2> <div class="wpb_text_column wpb_content_element vc_custom_1592398045046 custom_h5" > <div class="wpb_wrapper"> <h5>Covaris instruments powered by <a href="/afa-technology/">Adaptive Focused Acoustics® (AFA®)</a> are the well established and trusted high-throughput chromatin platforms available that allow chromatin shearing in a 96 well format. Sample types supported include <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010179.pdf">cultured cell lines</a>, <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010179.pdf">primary cells</a>, <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010385.pdf">fresh/ frozen tissues</a>, and <a href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/pn_010464.pdf">FFPE Fixed tissues</a>.</h5> <h5>AFA is widely used by scientists to decipher the regulatory potential of chromatin landscape at several layers including histone modifications, DNA methylation patterns as well as interactions with biomolecules like transcription factors, epigenetic regulators and ncRNAs. The Covaris technology has further been proven beneficial to restore multi-subunit complexes from chromatin and to study 3D chromatin organization.</h5> <h5>AFA is the method of choice in NuGEN’s Ovation® platform and Agilent’s SureSelectXT Methyl-Seq protocols for profiling 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). It is also cited as the preferred sample prep method for <a href="/wp/wp-content/uploads/2020/04/M020091_RevA_MeDIP.pdf" target="_blank" rel="noopener noreferrer">meDIP-Seq</a>, <a href="/wp/wp-content/uploads/2020/04/M020117-1.pdf" target="_blank" rel="noopener noreferrer">ChIP-Seq</a>, <a href="/wp/wp-content/uploads/2020/04/M020094_RevA_HiC-1.pdf" target="_blank" rel="noopener noreferrer">HI-C</a>, <a href="/wp/wp-content/uploads/2020/04/M020097.pdf" target="_blank" rel="noopener noreferrer">HI-ChIP</a>, <a href="/wp/wp-content/uploads/2020/04/M020092_RevA_ChIRP.pdf" target="_blank" rel="noopener noreferrer">ChIRP-Seq</a>, and other epigenomics applications.</h5> <p><a class="image-popup-no-margins" href="https://www.covaris.com/wp/wp-content/uploads/2020/03/EpigenomicsSolutions_v4.jpg"><br /> <img src="https://www.covaris.com/wp/wp-content/uploads/2020/03/EpigenomicsSolutions_v4.jpg" /><br /> </a></p> <h5 style="text-align: center;">Click here to download our Comprehensive Epigenomic Sample Prep Flyer</h5> </div> </div> <div class="vc_btn3-container category_accordion vc_btn3-center vc_custom_1623352450166 vc_do_btn" ><a style="background-color:#ffffff; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom" href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020133.pdf" title="" target="_blank">DOWNLOAD</a></div></div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1_quote vc_custom_1583730624487"><div class="wpb_column vc_column_container vc_col-sm-12"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1583730045887" > <div class="wpb_wrapper"> <p><img class="aligncenter" src="/wp/wp-content/uploads/2020/02/quote.png"" /></p> </div> </div> <h5 style="color: #2c2c2c;text-align: center" class="vc_custom_heading vc_do_custom_heading" >“In the past we struggled to get reproducible chromatin shearing to a size range suitable for ChIPseq experiments. However, we were relieved that using the Covaris E220 Focused-ultrasonicator enabled us to optimize a chromatin sample preparation protocol in less than 2 weeks. The fast optimization and high degree of reproducibility in shearing, without day to day or sample to sample variation, has made our scientific life much easier.”</h5><h6 style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading" >Stefanie Dichtl, PhD</h6><p style="color: #2d3e51;text-align: center" class="vc_custom_heading vc_do_custom_heading" >Max-Planck-Institut für Biochemie</p></div></div></div></div></div></div></div></div><div id="featured_product" class="vc_row wpb_row vc_row-fluid cmspage_menu vc_custom_1583494558716"><div class="wpb_column vc_column_container vc_col-sm-3"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1586353821519" >Featured Products</h2><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1 vc_custom_1583494563486 vc_row-o-equal-height vc_row-flex"><div class="wpb_column vc_column_container vc_col-sm-4"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1589195068106" > <div class="wpb_wrapper"> <p><img class="size-full wp-image-15527" src="https://www.covaris.com/wp/wp-content/uploads/2020/02/ChIPKit_blurred-1.jpg" alt="" width="200" height="134" /></p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-8"><div class="vc_column-inner"><div class="wpb_wrapper"><h4 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1583730724945" >truChIP Chromatin Shearing Kit with Formaldehyde</h4><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Optimized for the efficient and reproducible shearing of chromatin from cultured mammalian cells (suspension or adherent) using Covaris AFA Focused-ultrasonicators.</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1583730840995 vc_do_btn" ><a style="background-color:#ededed; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom vc_btn3-icon-right" href="/truchip-chromatin-shearing-kit-with-formaldehyde-pr520154" title="">READ MORE <i class="vc_btn3-icon fas fa-arrow-left"></i></a></div></div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1 vc_custom_1583494563486 vc_row-o-equal-height vc_row-flex"><div class="wpb_column vc_column_container vc_col-sm-4"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1583731721394" > <div class="wpb_wrapper"> <p><img class="size-full wp-image-15527" src="https://www.covaris.com/wp/wp-content/uploads/2020/02/ChIPKit_blurred-1.jpg" alt="" width="200" height="134" /></p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-8"><div class="vc_column-inner"><div class="wpb_wrapper"><h4 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1583730899070" >truChIP Chromatin Shearing Tissue Kit</h4><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Optimized for the efficient and reproducible shearing of chromatin from tissues (20-120mg). using Covaris AFA Focused-ultrasonicators.</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1583730994612 vc_do_btn" ><a style="background-color:#ededed; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom vc_btn3-icon-right" href="/truchip-chromatin-shearing-tissue-kit-with-formaldehyde-520237" title="">READ MORE <i class="vc_btn3-icon fas fa-arrow-left"></i></a></div></div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid instrument_lev_1 vc_custom_1583494563486 vc_row-o-equal-height vc_row-flex"><div class="wpb_column vc_column_container vc_col-sm-4"><div class="vc_column-inner"><div class="wpb_wrapper"> <div class="wpb_text_column wpb_content_element vc_custom_1583731743507" > <div class="wpb_wrapper"> <p><img class="size-full wp-image-15527" src="https://www.covaris.com/wp/wp-content/uploads/2020/02/ChIPKit_blurred-1.jpg" alt="" width="200" height="134" /></p> </div> </div> </div></div></div><div class="wpb_column vc_column_container vc_col-sm-8"><div class="vc_column-inner"><div class="wpb_wrapper"><h4 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1583731023204" >truChIP FFPE Chromatin Shearing Kit</h4><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading" >Optimized for the extraction and shearing of chromatin from formalin fixed paraffin embedded (FFPE) tissues using Covaris AFA Focused-ultrasonicators</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1583731122978 vc_do_btn" ><a style="background-color:#ededed; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom vc_btn3-icon-right" href="/truchip-ffpe-chromatin-shearing-kit-520257" title="">READ MORE <i class="vc_btn3-icon fas fa-arrow-left"></i></a></div></div></div></div></div></div></div></div></div><div id="resources" class="vc_row wpb_row vc_row-fluid cmspage_menu category_resources vc_custom_1586353035681"><div class="wpb_column vc_column_container vc_col-sm-3"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner vc_custom_1583492989135"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading vc_custom_1579697651435" >Resources</h2><div class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1583492996693 vc_row-o-equal-height vc_row-flex"><div class="sidebar_resources wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner"><div class="wpb_wrapper"><h5 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1583731338073" >Chromatin Shearing Protocols</h5><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1583731359943" >Protocols for using truChIP® Chromatin Shearing Kits with Covaris Adaptive Focused Acoustics (AFA) technology.</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1587800053581 vc_do_btn" ><a style="background-color:#ffffff; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom" href="/protocols?filter_tag=Chromatin%20Shearing%20Protocols" title="">LEARN MORE</a></div> <div class="wpb_text_column wpb_content_element vc_custom_1579697330020" > <div class="wpb_wrapper"> </div> </div> </div></div></div><div class="sidebar_resources wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner"><div class="wpb_wrapper"><h5 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1591218683263" >Chromatin Shearing Application Notes</h5><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1583731429983" >Case studies, tech notes and app notes to support chromatin shearing workflows powered by Covaris Adaptive Focused Acoustics (AFA) technology.</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1587800329467 vc_do_btn" ><a style="background-color:#ffffff; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom" href="/application-technical-notes?filter_tag=Epigenetic%20Workflows" title="">LEARN MORE</a></div></div></div></div></div><div class="vc_row wpb_row vc_inner vc_row-fluid vc_custom_1583492996693 vc_row-o-equal-height vc_row-flex"><div class="sidebar_resources wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner"><div class="wpb_wrapper"><h5 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1682607422342" >Chromatin Shearing Videos</h5><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1682607433062" >Learn more about Chromatin Shearing with Covaris through customer driven presentations and webinars.</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1682607451460 vc_do_btn" ><a style="background-color:#ffffff; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom" href="/videos?filter_tag=Chromatin%20and%20ChIP" title="">LEARN MORE</a></div> <div class="wpb_text_column wpb_content_element vc_custom_1579697330020" > <div class="wpb_wrapper"> </div> </div> </div></div></div><div class="sidebar_resources wpb_column vc_column_container vc_col-sm-6"><div class="vc_column-inner"><div class="wpb_wrapper"><h5 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1682607466178" >Chromatin Preparation and Shearing Brochure</h5><h6 style="color: #020202;text-align: left" class="vc_custom_heading vc_do_custom_heading custom_h5 vc_custom_1682607480930" >A Presentation of Chromatin Applications Enabled by AFA-energetics®</h6><div class="vc_btn3-container vc_btn3-inline vc_custom_1682607567313 vc_do_btn" ><a style="background-color:#ffffff; color:#015cb8;" class="vc_general vc_btn3 vc_btn3-size-md vc_btn3-shape-rounded vc_btn3-style-custom" href="https://www.covaris.com/wp/wp-content/uploads/resources_pdf/M020139.pdf" title="">DOWNLOAD</a></div></div></div></div></div></div></div></div></div><div id="references" class="vc_row wpb_row vc_row-fluid cmspage_menu"><div class="wpb_column vc_column_container vc_col-sm-3"><div class="vc_column-inner"><div class="wpb_wrapper"></div></div></div><div class="wpb_column vc_column_container vc_col-sm-9"><div class="vc_column-inner"><div class="wpb_wrapper"><h2 style="color: #2d3e51;text-align: left" class="vc_custom_heading vc_do_custom_heading" >References</h2> <div class="wpb_text_column wpb_content_element custom_h5" > <div class="wpb_wrapper"> <ol> <li>Caliskan M, Manduchi E, Rao S et.al. 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