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ear</title> <meta name="description" content="Search results for: mouse ear"> <meta name="keywords" content="mouse ear"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" 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id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="mouse ear"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 257</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: mouse ear</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">257</span> Advanced Mouse Cursor Control and Speech Recognition Module</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Prasad%20Kalagura">Prasad Kalagura</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20Veeresh%20kumar"> B. Veeresh kumar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We constructed an interface system that would allow a similarly paralyzed user to interact with a computer with almost full functional capability. A real-time tracking algorithm is implemented based on adaptive skin detection and motion analysis. The clicking of the mouse is activated by the user's eye blinking through a sensor. The keyboard function is implemented by voice recognition kit. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=embedded%20ARM7%20processor" title="embedded ARM7 processor">embedded ARM7 processor</a>, <a href="https://publications.waset.org/abstracts/search?q=mouse%20pointer%20control" title=" mouse pointer control"> mouse pointer control</a>, <a href="https://publications.waset.org/abstracts/search?q=voice%20recognition" title=" voice recognition "> voice recognition </a> </p> <a href="https://publications.waset.org/abstracts/31757/advanced-mouse-cursor-control-and-speech-recognition-module" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/31757.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">578</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">256</span> Study on 3D FE Analysis on Normal and Osteoporosis Mouse Models Based on 3-Point Bending Tests</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tae-min%20Byun">Tae-min Byun</a>, <a href="https://publications.waset.org/abstracts/search?q=Chang-soo%20Chon"> Chang-soo Chon</a>, <a href="https://publications.waset.org/abstracts/search?q=Dong-hyun%20Seo"> Dong-hyun Seo</a>, <a href="https://publications.waset.org/abstracts/search?q=Han-sung%20Kim"> Han-sung Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Bum-mo%20Ahn"> Bum-mo Ahn</a>, <a href="https://publications.waset.org/abstracts/search?q=Hui-suk%20Yun"> Hui-suk Yun</a>, <a href="https://publications.waset.org/abstracts/search?q=Cheolwoong%20Ko"> Cheolwoong Ko</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this study, a 3-point bending computational analysis of normal and osteoporosis mouse models was performed based on the Micro-CT image information of the femurs. The finite element analysis (FEA) found 1.68 N (normal group) and 1.39 N (osteoporosis group) in the average maximum force, and 4.32 N/mm (normal group) and 3.56 N/mm (osteoporosis group) in the average stiffness. In the comparison of the 3-point bending test results, the maximum force and the stiffness were different about 9.4 times in the normal group and about 11.2 times in the osteoporosis group. The difference between the analysis and the test was greatly significant and this result demonstrated improvement points of the material properties applied to the computational analysis of this study. For the next study, the material properties of the mouse femur will be supplemented through additional computational analysis and test. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=3-point%20bending%20test" title="3-point bending test">3-point bending test</a>, <a href="https://publications.waset.org/abstracts/search?q=mouse" title=" mouse"> mouse</a>, <a href="https://publications.waset.org/abstracts/search?q=osteoporosis" title=" osteoporosis"> osteoporosis</a>, <a href="https://publications.waset.org/abstracts/search?q=FEA" title=" FEA"> FEA</a> </p> <a href="https://publications.waset.org/abstracts/54813/study-on-3d-fe-analysis-on-normal-and-osteoporosis-mouse-models-based-on-3-point-bending-tests" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54813.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">351</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">255</span> Alteration of Bone Strength in Osteoporosis of Mouse Femora: Computational Study Based on Micro CT Images</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Changsoo%20Chon">Changsoo Chon</a>, <a href="https://publications.waset.org/abstracts/search?q=Sangkuy%20Han"> Sangkuy Han</a>, <a href="https://publications.waset.org/abstracts/search?q=Donghyun%20Seo"> Donghyun Seo</a>, <a href="https://publications.waset.org/abstracts/search?q=Jihyung%20Park"> Jihyung Park</a>, <a href="https://publications.waset.org/abstracts/search?q=Bokku%20Kang"> Bokku Kang</a>, <a href="https://publications.waset.org/abstracts/search?q=Hansung%20Kim"> Hansung Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Keyoungjin%20Chun"> Keyoungjin Chun</a>, <a href="https://publications.waset.org/abstracts/search?q=Cheolwoong%20Ko"> Cheolwoong Ko</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The purpose of the study is to develop a finite element model based on 3D bone structural images of Micro-CT and to analyze the stress distribution for the osteoporosis mouse femora. In this study, results of finite element analysis show that the early osteoporosis of mouse model decreased a bone density in trabecular region; however, the bone density in cortical region increased. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=micro-CT" title="micro-CT">micro-CT</a>, <a href="https://publications.waset.org/abstracts/search?q=finite%20element%20analysis" title=" finite element analysis"> finite element analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=osteoporosis" title=" osteoporosis"> osteoporosis</a>, <a href="https://publications.waset.org/abstracts/search?q=bone%20strength" title=" bone strength"> bone strength</a> </p> <a href="https://publications.waset.org/abstracts/48362/alteration-of-bone-strength-in-osteoporosis-of-mouse-femora-computational-study-based-on-micro-ct-images" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/48362.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">363</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">254</span> Semi-Automated Tracking of Vibrissal Movements in Free-Moving Rodents Captured by High-Speed Videos</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hyun%20June%20Kim">Hyun June Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=Tailong%20Shi"> Tailong Shi</a>, <a href="https://publications.waset.org/abstracts/search?q=Seden%20Akdagli"> Seden Akdagli</a>, <a href="https://publications.waset.org/abstracts/search?q=Sam%20Most"> Sam Most</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuling%20Yan"> Yuling Yan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Quantitative analysis of mouse whisker movement can be used to study functional recovery and regeneration of facial nerve after an injury. However, it is challenging to accurately track mouse whisker movements, and most whisker tracking methods require manual intervention, e.g. fixing the head of the mouse during a study. Here we describe a semi-automated image processing method that is applied to high-speed video recordings of free-moving mice to track whisker movements. We first track the head movement of a mouse by delineating the lower head contour frame-by-frame to locate and determine the orientation of its head. Then, a region of interest is identified for each frame, with subsequent application of the Hough transform to track individual whisker movements on each side of the head. Our approach is used to examine the functional recovery of damaged facial nerves in mice over a course of 21 days. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=mystacial%20macrovibrissae" title="mystacial macrovibrissae">mystacial macrovibrissae</a>, <a href="https://publications.waset.org/abstracts/search?q=whisker%20tracking" title=" whisker tracking"> whisker tracking</a>, <a href="https://publications.waset.org/abstracts/search?q=head%20tracking" title=" head tracking"> head tracking</a>, <a href="https://publications.waset.org/abstracts/search?q=facial%20nerve%20recovery" title=" facial nerve recovery "> facial nerve recovery </a> </p> <a href="https://publications.waset.org/abstracts/20157/semi-automated-tracking-of-vibrissal-movements-in-free-moving-rodents-captured-by-high-speed-videos" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20157.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">590</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">253</span> The Effect of Visfatin on Pregnant Mouse Myometrial Contractility in vitro</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Seham%20Alsaif">Seham Alsaif</a>, <a href="https://publications.waset.org/abstracts/search?q=Susan%20Wray"> Susan Wray</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Obesity is a worldwide disorder influencing women’s health and childbearing. There is a close relation between obesity and pregnancy related complications. Dyslipidemia and adipokine dysregulation are core environmental changes that may mechanistically link these complications with obesity in pregnant women. We have previously found that visfatin has a relaxant effect on mouse, rat and human myometrial contractility. We hypothesised that visfatin inhibits mouse myometrial contractility through the NAD+ pathway. This study was designed to examine the mechanism of action of visfatin on myometrial contractility. To examine the NAD+ pathway, FK866 which is a potent inhibitor of NAD+ biosynthesis was used. Methods: Myometrial strips from term pregnant mice were dissected, superfused with physiological saline and the effects of visfatin (10nM) on oxytocin-induced contractions (0.5nM) alone and after the infusion of FK866 (10uM) were studied. After regular contractions were established, contractility was examined for control (100%) and test response at 37 °C for 10 min each. Results: FK866 was found to inhibit the effect of visfatin on myometrial contractility (the AUC increased from 89±2% of control, P=0.0009 for visfatin alone to 97±4% of control, P>0.05 for visfatin combined with FK866, n=8). In conclusion, NAD+ pathway appears to be involved in the mechanism of action of visfatin on mouse myometrium. This could have a role in making new targets to prevent obesity-related complications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=myometrium" title="myometrium">myometrium</a>, <a href="https://publications.waset.org/abstracts/search?q=obesity" title=" obesity"> obesity</a>, <a href="https://publications.waset.org/abstracts/search?q=oxytocin" title=" oxytocin"> oxytocin</a>, <a href="https://publications.waset.org/abstracts/search?q=pregnancy" title=" pregnancy"> pregnancy</a>, <a href="https://publications.waset.org/abstracts/search?q=visfatin" title=" visfatin"> visfatin</a> </p> <a href="https://publications.waset.org/abstracts/81512/the-effect-of-visfatin-on-pregnant-mouse-myometrial-contractility-in-vitro" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/81512.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">177</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">252</span> Study of Effects of Hydro-Alcoholic Extract of Asparagus Root (Asparagus officinalis) Ontestes Spermyogenesis Index of Laboratory Mouse</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hamid%20Karimi">Hamid Karimi</a>, <a href="https://publications.waset.org/abstracts/search?q=Naegar%20Mahdavi"> Naegar Mahdavi</a>, <a href="https://publications.waset.org/abstracts/search?q=Hossein%20Tayefi%20Nasrabadi"> Hossein Tayefi Nasrabadi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Spermatozoids production rate and its quality are more important factors in the diagnosis of infertility. Also, spematozids activity have a more important role in fertilization. Some medicinal plants as Asparagus(Asparagus officinalis) has many antioxidant component. Therefore, They can affect testes tissue to production more and high-quality spermatozoids. In this survey, Asparagus root extract is studied on spermatogenesis index in the laboratory mouse testes. Hydro-alcoholic extract of asparagus root is prepared and examined on four group of the mature male mouse. Blank group without extract, group 1,100ml/kg dose, group 2, 200 ml/kg dose and group 3, 300ml/kg dose. Then, mice are euthanized, and testes are removed. Testes are weighted, and paraffinized blocks are prepared. TDI(Tubular Differentiation Index) and SPI(Spermiation Index) are studied on histological sections by light microscope. This study results were showed that TDI and SPI in treatments groups with 200 and 300 ml/kg dose had significant enhancement (P<0.05). Consequently, Extract of Asparagus root can enhance spermatozoid production and, therefore, cause improve fertility in male laboratory mice. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=histology" title="histology">histology</a>, <a href="https://publications.waset.org/abstracts/search?q=spermatozoid" title=" spermatozoid"> spermatozoid</a>, <a href="https://publications.waset.org/abstracts/search?q=ASP%20%5Baragus" title=" ASP [aragus"> ASP [aragus</a>, <a href="https://publications.waset.org/abstracts/search?q=testes" title=" testes"> testes</a> </p> <a href="https://publications.waset.org/abstracts/142815/study-of-effects-of-hydro-alcoholic-extract-of-asparagus-root-asparagus-officinalis-ontestes-spermyogenesis-index-of-laboratory-mouse" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/142815.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">165</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">251</span> Preparation of Flurbiprofen Derivative for Enhanced Brain Penetration</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jungkyun%20Im">Jungkyun Im</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Nonsteroidal anti-inflammatory drugs (NSAIDs) are effective for relieving pain and reducing inflammation. They are nonselective inhibitors of two isoforms of COX, cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), and thereby inhibiting the production of hormone-like lipid compounds such as, prostaglandins and thromboxanes which cause inflammation, pain, fever, platelet aggregation, etc. In addition, recently there are many research articles reporting the neuroprotective effect of NSAIDs in neurodegenerative diseases, such as Alzheimer’s disease (AD) and Parkinson’s disease (PD). However, the clinical use of NSAIDs in these diseases is limited by low brain distribution. Therefore, in order to assist the in-depth investigation on the pharmaceutical mechanism of flurbiprofen in neuroprotection and to make flurbiprofen a more potent drug to prevent or alleviate neurodegenerative diseases, delivery of flurbiprofen to brain should be effective and sufficient amount of flurbiprofen must penetrate the BBB thus gaining access into the patient’s brain. We have recently developed several types of guanidine-rich molecular carriers with high molecular weights and good water solubility that readily cross the blood-brain barrier (BBB) and display efficient distributions in the mouse brain. The G8 (having eight guanidine groups) molecular carrier based on D-sorbitol was found to be very effective in delivering anticancer drugs to a mouse brain. In the present study, employing the same molecular carrier, we prepared the flurbiprofen conjugate and studied its BBB permeation by mouse tissue distribution study. Flurbiprofen was attached to a molecular carrier with a fluorescein probe and multiple terminal guanidiniums. The conjugate was found to internalize into live cells and readily cross the BBB to enter the mouse brain. Our novel synthetic flurbiprofen conjugate will hopefully delivery NSAIDs into brain, and is therefore applicable to the neurodegenerative diseases treatment or prevention. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=flurbiprofen" title="flurbiprofen">flurbiprofen</a>, <a href="https://publications.waset.org/abstracts/search?q=drug%20delivery" title=" drug delivery"> drug delivery</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20carrier" title=" molecular carrier"> molecular carrier</a>, <a href="https://publications.waset.org/abstracts/search?q=organic%20synthesis" title=" organic synthesis"> organic synthesis</a> </p> <a href="https://publications.waset.org/abstracts/78699/preparation-of-flurbiprofen-derivative-for-enhanced-brain-penetration" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/78699.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">231</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">250</span> Insulin Secretory Actions of Spirulina platensis in Perfused Rat Pancreas, Isolated Mouse Islets, and Clonal Pancreatic Β-Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jma%20Hannan">Jma Hannan</a>, <a href="https://publications.waset.org/abstracts/search?q=Prawej%20Ansari"> Prawej Ansari</a>, <a href="https://publications.waset.org/abstracts/search?q=Yasser%20H.%20A.%20Abdel-Wahab"> Yasser H. A. Abdel-Wahab</a>, <a href="https://publications.waset.org/abstracts/search?q=Peter%20R.%20Flatt"> Peter R. Flatt</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Spirulina platensis (SP, Blue-green algae) have been accepted as a supplement for the treatment of pre and post-diabetes. The present study investigated the effects of butanol fraction from ethanol extract of S. platensis on insulin release from BRIN BD11 cells, isolated mouse islets, and perfused rat pancreas, as well as glucose homeostasis in type 2 diabetic rats and their molecular pathways. In a dose-dependent manner, S. platensis increased insulin release from mouse islets and pancreatic β-cells. The extract also elevated insulin release in perfused rat pancreas. Glucose, isobutylmethylxanthine, tolbutamide, and a depolarizing concentration of KCl significantly potentiated insulin release from BRIN BD11 cells. The effect of diazoxide and verapamil, as well as the absence of extracellular Ca2+ showed a reduction in insulin secretion. When administered orally together with glucose (2.5g/kg bw), S. platensis extract improved fasting and postprandial blood glucose in type 2 diabetes. These data suggest that the anti-diabetic activity of S. platensis is partly mediated by insulin secretion via the KATP channel-dependent pathway/the intracellular cAMP pathway. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Insulin" title="Insulin">Insulin</a>, <a href="https://publications.waset.org/abstracts/search?q=glucose" title=" glucose"> glucose</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20platensis" title=" S. platensis"> S. platensis</a>, <a href="https://publications.waset.org/abstracts/search?q=type%202%20diabetes" title=" type 2 diabetes"> type 2 diabetes</a>, <a href="https://publications.waset.org/abstracts/search?q=medicinal%20plants" title=" medicinal plants"> medicinal plants</a> </p> <a href="https://publications.waset.org/abstracts/154092/insulin-secretory-actions-of-spirulina-platensis-in-perfused-rat-pancreas-isolated-mouse-islets-and-clonal-pancreatic-b-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/154092.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">112</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">249</span> Gaming Mouse Redesign Based on Evaluation of Pragmatic and Hedonic Aspects of User Experience</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Thedy%20Yogasara">Thedy Yogasara</a>, <a href="https://publications.waset.org/abstracts/search?q=Fredy%20Agus"> Fredy Agus</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In designing a product, it is currently crucial to focus not only on the product’s usability based on performance measures, but also on user experience (UX) that includes pragmatic and hedonic aspects of product use. These aspects play a significant role in fulfillment of user needs, both functionally and psychologically. Pragmatic quality refers to as product’s perceived ability to support the fulfillment of behavioral goals. It is closely linked to functionality and usability of the product. In contrast, hedonic quality is product’s perceived ability to support the fulfillment of psychological needs. Hedonic quality relates to the pleasure of ownership and use of the product, including stimulation for personal development and communication of user’s identity to others through the product. This study evaluates the pragmatic and hedonic aspects of gaming mice G600 and Razer Krait using AttrakDiff tool to create an improved design that is able to generate positive UX. AttrakDiff is a method that measures pragmatic and hedonic scores of a product with a scale between -3 to +3 through four attributes (i.e. Pragmatic Quality, Hedonic Quality-Identification, Hedonic Quality-Stimulation, and Attractiveness), represented by 28 pairs of opposite words. Based on data gathered from 15 participants, it is identified that gaming mouse G600 needs to be redesigned because of its low grades (pragmatic score: -0.838, hedonic score: 1, attractiveness score: 0.771). The redesign process focuses on the attributes with poor scores and takes into account improvement suggestions collected from interview with the participants. The redesigned mouse G600 is evaluated using the previous method. The result shows higher scores in pragmatic quality (1.929), hedonic quality (1.703), and attractiveness (1.667), indicating that the redesigned mouse is more capable of creating pleasurable experience of product use. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=AttrakDiff" title="AttrakDiff">AttrakDiff</a>, <a href="https://publications.waset.org/abstracts/search?q=hedonic%20aspect" title=" hedonic aspect"> hedonic aspect</a>, <a href="https://publications.waset.org/abstracts/search?q=pragmatic%20aspect" title=" pragmatic aspect"> pragmatic aspect</a>, <a href="https://publications.waset.org/abstracts/search?q=product%20design" title=" product design"> product design</a>, <a href="https://publications.waset.org/abstracts/search?q=user%20experience" title=" user experience"> user experience</a> </p> <a href="https://publications.waset.org/abstracts/92984/gaming-mouse-redesign-based-on-evaluation-of-pragmatic-and-hedonic-aspects-of-user-experience" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/92984.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">157</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">248</span> Integrated Gesture and Voice-Activated Mouse Control System</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Dev%20Pratap%20Singh">Dev Pratap Singh</a>, <a href="https://publications.waset.org/abstracts/search?q=Harshika%20Hasija"> Harshika Hasija</a>, <a href="https://publications.waset.org/abstracts/search?q=Ashwini%20S."> Ashwini S.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The project aims to provide a touchless, intuitive interface for human-computer interaction, enabling users to control their computers using hand gestures and voice commands. The system leverages advanced computer vision techniques using the Media Pipe framework and OpenCV to detect and interpret real-time hand gestures, transforming them into mouse actions such as clicking, dragging, and scrolling. Additionally, the integration of a voice assistant powered by the speech recognition library allows for seamless execution of tasks like web searches, location navigation, and gesture control in the system through voice commands. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gesture%20recognition" title="gesture recognition">gesture recognition</a>, <a href="https://publications.waset.org/abstracts/search?q=hand%20tracking" title=" hand tracking"> hand tracking</a>, <a href="https://publications.waset.org/abstracts/search?q=machine%20learning" title=" machine learning"> machine learning</a>, <a href="https://publications.waset.org/abstracts/search?q=convolutional%20neural%20networks" title=" convolutional neural networks"> convolutional neural networks</a>, <a href="https://publications.waset.org/abstracts/search?q=natural%20language%20processing" title=" natural language processing"> natural language processing</a>, <a href="https://publications.waset.org/abstracts/search?q=voice%20assistant" title=" voice assistant"> voice assistant</a> </p> <a href="https://publications.waset.org/abstracts/193896/integrated-gesture-and-voice-activated-mouse-control-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/193896.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">10</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">247</span> Anti-Inflammatory Effect of Carvedilol 1% Ointment in Topical Application to the Animal Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Berina%20Pilipovi%C4%87">Berina Pilipović</a>, <a href="https://publications.waset.org/abstracts/search?q=Sa%C5%A1a%20Pilipovi%C4%87"> Saša Pilipović</a>, <a href="https://publications.waset.org/abstracts/search?q=Maja%20Pa%C5%A1i%C4%87-Kulenovi%C4%87"> Maja Pašić-Kulenović</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Inflammation is the body's response to impaired homeostasis caused by infection, injury or trauma resulting in systemic and local effects. Inflammation causes the body's response to injury and is characterized by a series of events including inflammatory response, response to pain receptors and the recovery process. Inflammation can be acute and chronic. The inflammatory response is described in three different phases. Free radical is an atom or molecule that has the unpaired electron and is therefore generally very reactive chemical species. Biologically important example of reaction with free radicals is called Lipid peroxidation (LP). Lipid peroxidation reactions occur in biological membranes, and if at the outset is not stopped with the action of antioxidants, it will bring damage to the membrane, which results in partial or complete loss of their physiological functions. Calcium antagonists and beta-adrenergic receptor antagonists are known drugs, and for many years and widely used in the treatment of cardiovascular diseases. Some of these compounds also show antioxidant activity. The mechanism of antioxidant activities of calcium antagonists and beta-blockers is unknown, since their structure varies widely. This research investigated the possible local anti-inflammatory activity of ointments containing 1% carvedilol in the white petrolatum USP. Ear inflammation was induced by 3% croton oil acetone solution, in quantity of 10 µl on both mouse ears. Albino Swiss mouse (n = 8) are treated with 2.5 mg/ear ointment, and control group was treated on the same way as previous with hydrocortisone 1% ointment (2.5 mg/ear). The other ear of the same animal was used as control one. Ointments were administered once per day, on the left ear. After treatment, ears were observed for three days. After three days, we measured mass (mg) of 6 mm ear punch of treated and controlled ears. The results of testing anti-inflammatory effects of ointments with carvedilol in the mouse ear model show stronger observed effect than ointment with 1% hydrocortisone in the same basis. Identical results were confirmed by the difference between the mass of 6 mm ears punch. The results were also confirmed by histological examination. Ointments with carvedilol showed significant reduction of the inflammation process caused by croton oil on the mouse inflammation model. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antioxidant" title="antioxidant">antioxidant</a>, <a href="https://publications.waset.org/abstracts/search?q=carvedilol" title=" carvedilol"> carvedilol</a>, <a href="https://publications.waset.org/abstracts/search?q=inflammation" title=" inflammation"> inflammation</a>, <a href="https://publications.waset.org/abstracts/search?q=mouse%20ear" title=" mouse ear"> mouse ear</a> </p> <a href="https://publications.waset.org/abstracts/68212/anti-inflammatory-effect-of-carvedilol-1-ointment-in-topical-application-to-the-animal-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/68212.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">234</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">246</span> Information Retrieval from Internet Using Hand Gestures</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aniket%20S.%20Joshi">Aniket S. Joshi</a>, <a href="https://publications.waset.org/abstracts/search?q=Aditya%20R.%20Mane"> Aditya R. Mane</a>, <a href="https://publications.waset.org/abstracts/search?q=Arjun%20Tukaram"> Arjun Tukaram </a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the 21st century, in the era of e-world, people are continuously getting updated by daily information such as weather conditions, news, stock exchange market updates, new projects, cricket updates, sports and other such applications. In the busy situation, they want this information on the little use of keyboard, time. Today in order to get such information user have to repeat same mouse and keyboard actions which includes time and inconvenience. In India due to rural background many people are not much familiar about the use of computer and internet also. Also in small clinics, small offices, and hotels and in the airport there should be a system which retrieves daily information with the minimum use of keyboard and mouse actions. We plan to design application based project that can easily retrieve information with minimum use of keyboard and mouse actions and make our task more convenient and easier. This can be possible with an image processing application which takes real time hand gestures which will get matched by system and retrieve information. Once selected the functions with hand gestures, the system will report action information to user. In this project we use real time hand gesture movements to select required option which is stored on the screen in the form of RSS Feeds. Gesture will select the required option and the information will be popped and we got the information. A real time hand gesture makes the application handier and easier to use. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hand%20detection" title="hand detection">hand detection</a>, <a href="https://publications.waset.org/abstracts/search?q=hand%20tracking" title=" hand tracking"> hand tracking</a>, <a href="https://publications.waset.org/abstracts/search?q=hand%20gesture%20recognition" title=" hand gesture recognition"> hand gesture recognition</a>, <a href="https://publications.waset.org/abstracts/search?q=HSV%20color%20model" title=" HSV color model"> HSV color model</a>, <a href="https://publications.waset.org/abstracts/search?q=Blob%20detection" title=" Blob detection"> Blob detection</a> </p> <a href="https://publications.waset.org/abstracts/29069/information-retrieval-from-internet-using-hand-gestures" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29069.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">289</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">245</span> Antiasthmatic Effect of Kankasava in OVA-Induced Asthma Mouse Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bharti%20Ahirwar">Bharti Ahirwar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The main object of this study was to evaluate the effect of kankasava on OVA-induced asthma in mouse model. Present study has demonstrated that kankasava exhibited an antiasthmatic effect by attenuated AHR and reducing level of IgE, IL-5, and IL-13, in both serum and BALF in OVA induced asthmatic mice. Effect of kankasav on airway responsiveness was obtained by monitoring the enhanced pen value . Kankasava significantly reduced AHR can be explained, in part, by reduction in both IgE overexoression and cytokine levels. Kankasava significantly decreased IL-4, IL-5, and IL-13 in BALF indicate that it may suppress the excess activity of T-cells and Th2 cytokines, which are implicated in the pathogenesis of allergic asthma, and consequently restore the Th1/Th2 imbalance of the immune system. In summary, we hypothesize that kankasava effectively suppressed elevations in IgE and cytokines levels, AHR, and mucus overproduction in mice with OVA-induced asthma suggested kankasava could be effective in immunological and pharmacological modulation of allergic asthma. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=asthma" title="asthma">asthma</a>, <a href="https://publications.waset.org/abstracts/search?q=ayurveda" title=" ayurveda"> ayurveda</a>, <a href="https://publications.waset.org/abstracts/search?q=kankasava" title=" kankasava"> kankasava</a>, <a href="https://publications.waset.org/abstracts/search?q=cytokine" title=" cytokine"> cytokine</a> </p> <a href="https://publications.waset.org/abstracts/44415/antiasthmatic-effect-of-kankasava-in-ova-induced-asthma-mouse-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44415.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">312</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">244</span> The Immediate Effects of Thrust Manipulation for Thoracic Hyperkyphosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Betul%20Taspinar">Betul Taspinar</a>, <a href="https://publications.waset.org/abstracts/search?q=Eda%20O.%20Okur"> Eda O. Okur</a>, <a href="https://publications.waset.org/abstracts/search?q=Ismail%20Saracoglu"> Ismail Saracoglu</a>, <a href="https://publications.waset.org/abstracts/search?q=Ismail%20Okur"> Ismail Okur</a>, <a href="https://publications.waset.org/abstracts/search?q=Ferruh%20Taspinar"> Ferruh Taspinar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Thoracic hyperkyphosis, is a well-known spinal phenomenon, refers to an excessive curvature (> 40 degrees) of the thoracic spine. The aim of this study was to explore the effectiveness of thrust manipulation on thoracic spine alignment. 31 young adults with hyperkyphosis diagnosed with Spinal Mouse® device were randomly assigned either thrust manipulation group (n=16, 11 female, 5 male) or sham manipulation group (n=15, 8 female, 7 male). Thrust and sham manipulations were performed by a blinded physiotherapist who is a certificated expert in musculoskeletal physiotherapy. Thoracic kyphosis degree was measured after the interventions via Spinal Mouse®. Wilcoxon test was used to analyse the data obtained before and after the manipulation for each group, whereas Mann-Whitney U test was used to compare the groups. The mean of baseline thoracic kyphosis degrees in thrust and sham groups were 50.69 o ± 7.73 and 48.27o ± 6.43, respectively. There was no statistically significant difference between groups in terms of initial thoracic kyphosis degrees (p=0.51). After the interventions, the mean of thoracic kyphosis degree in thrust and sham groups were measured as 44.06o ± 6.99 and 48.93o ± 6.57 respectively (p=0.03). There was no statistically significant difference between before and after interventions in sham group (p=0.33), while the mean of thoracic kyphosis degree in thrust group decreased significantly (p=0.00). Thrust manipulation can attenuate thoracic hyperkyphosis immediately in young adults by not using placebo effect. Manipulation might provide accurate proprioceptive (sensory) input to the spine joints and reduce kyphosis by restoring normal segment mobility. Therefore thoracic manipulation might be included in the physiotherapy programs to treat hyperkyphosis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=hyperkyphosis" title="hyperkyphosis">hyperkyphosis</a>, <a href="https://publications.waset.org/abstracts/search?q=manual%20therapy" title=" manual therapy"> manual therapy</a>, <a href="https://publications.waset.org/abstracts/search?q=spinal%20mouse" title=" spinal mouse"> spinal mouse</a>, <a href="https://publications.waset.org/abstracts/search?q=physiotherapy" title=" physiotherapy"> physiotherapy</a> </p> <a href="https://publications.waset.org/abstracts/60263/the-immediate-effects-of-thrust-manipulation-for-thoracic-hyperkyphosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/60263.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">344</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">243</span> Autophagy Suppresses Bladder Tumor Formation in a Mouse Orthotopic Bladder Tumor Formation Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Wan-Ting%20Kuo">Wan-Ting Kuo</a>, <a href="https://publications.waset.org/abstracts/search?q=Yi-Wen%20Liu"> Yi-Wen Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Hsiao-Sheng%20Liu"> Hsiao-Sheng Liu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Annual incidence of bladder cancer increases in the world and occurs frequently in the male. Most common type is transitional cell carcinoma (TCC) which is treated by transurethral resection followed by intravesical administration of agents. In clinical treatment of bladder cancer, chemotherapeutic drugs-induced apoptosis is always used in patients. However, cancers usually develop resistance to chemotherapeutic drugs and often lead to aggressive tumors with worse clinical outcomes. Approximate 70% TCC recurs and 30% recurrent tumors progress to high-grade invasive tumors, indicating that new therapeutic agents are urgently needed to improve the successful rate of overall treatment. Nonapoptotic program cell death may assist to overcome worse clinical outcomes. Autophagy which is one of the nonapoptotic pathways provides another option for bladder cancer patients. Autophagy is reported as a potent anticancer therapy in some cancers. First of all, we established a mouse orthotopic bladder tumor formation model in order to create a similar tumor microenvironment. IVIS system and micro-ultrasound were utilized to noninvasively monitor tumor formation. In addition, we carried out intravesical treatment in our animal model to be consistent with human clinical treatment. In our study, we carried out intravesical instillation of the autophagy inducer in mouse orthotopic bladder tumor to observe tumor formation by noninvasive IVIS system and micro-ultrasound. Our results showed that bladder tumor formation is suppressed by the autophagy inducer, and there are no significant side effects in the physiology of mice. Furthermore, the autophagy inducer upregulated autophagy in bladder tissues of the treated mice was confirmed by Western blot, immunohistochemistry, and immunofluorescence. In conclusion, we reveal that a novel autophagy inducer with low side effects suppresses bladder tumor formation in our mouse orthotopic bladder tumor model, and it provides another therapeutic approach in bladder cancer patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bladder%20cancer" title="bladder cancer">bladder cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=transitional%20cell%20carcinoma" title=" transitional cell carcinoma"> transitional cell carcinoma</a>, <a href="https://publications.waset.org/abstracts/search?q=orthotopic%20bladder%20tumor%20formation%20model" title=" orthotopic bladder tumor formation model"> orthotopic bladder tumor formation model</a>, <a href="https://publications.waset.org/abstracts/search?q=autophagy" title=" autophagy"> autophagy</a> </p> <a href="https://publications.waset.org/abstracts/56139/autophagy-suppresses-bladder-tumor-formation-in-a-mouse-orthotopic-bladder-tumor-formation-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56139.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">177</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">242</span> Transcranial Electric Field Treatments on Redox-Toxic Iron Deposits in Transgenic Alzheimer’s Disease Mouse Models: The Electroceutical Targeting of Alzheimer’s Disease</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Choi%20Younshick">Choi Younshick</a>, <a href="https://publications.waset.org/abstracts/search?q=Lee%20Wonseok"> Lee Wonseok</a>, <a href="https://publications.waset.org/abstracts/search?q=Lee%20Jaemeun"> Lee Jaemeun</a>, <a href="https://publications.waset.org/abstracts/search?q=Park%20Sun-Hyun"> Park Sun-Hyun</a>, <a href="https://publications.waset.org/abstracts/search?q=Kim%20Sunwoung"> Kim Sunwoung</a>, <a href="https://publications.waset.org/abstracts/search?q=Park%20Sua"> Park Sua</a>, <a href="https://publications.waset.org/abstracts/search?q=Kim%20Eun%20Ho"> Kim Eun Ho</a>, <a href="https://publications.waset.org/abstracts/search?q=Kim%20Jong-Ki"> Kim Jong-Ki</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Iron accumulation in the brain accelerates Alzheimer’s disease progression. To cure iron toxicity, we assessed the therapeutic effects of noncontact transcranial electric field stimulation to the brain on toxic iron deposits in either the Aβ-fibril structure or the Aβ plaque in a mouse model of Alzheimer’s disease (AD). A capacitive electrode-based alternating electric field (AEF) was applied to a suspension of magnetite (Fe₃O₄) to measure the field-sensitized electro-Fenton effect and resultant reactive oxygen species (ROS) generation. The increase in ROS generation compared to the untreated control was both exposure-time and AEF-frequency dependent. The frequency-specific exposure of AEF to 0.7–1.4 V/cm on a magnetite-bound Aβ-fibril or a transgenic Alzheimer’s disease (AD) mouse model revealed the removal of intraplaque ferrous magnetite iron deposit and Aβ-plaque burden together at the same time compared to the untreated control. The results of the behavioral tests show an improvement in impaired cognitive function following AEF treatment on the AD mouse model. Western blot assay found some disease-modifying biological responses, including down-regulating ferroptosis, neuroinflammation and reactive astrocytes that eventually made cognitive improvement feasible. Tissue clearing and 3D-imaging analysis revealed no induced damage to the neuronal structures of normal brain tissue following AEF treatment. In conclusion, our results suggest that the effective degradation of magnetite-bound amyloid fibrils or plaques in the AD brain by the electro-Fenton effect from electric field-sensitized magnetite offers a potential electroceutical treatment option for AD. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electroceutical" title="electroceutical">electroceutical</a>, <a href="https://publications.waset.org/abstracts/search?q=intraplaque%20magnetite" title=" intraplaque magnetite"> intraplaque magnetite</a>, <a href="https://publications.waset.org/abstracts/search?q=alzheimer%E2%80%99s%20disease" title=" alzheimer’s disease"> alzheimer’s disease</a>, <a href="https://publications.waset.org/abstracts/search?q=transcranial%20electric%20field" title=" transcranial electric field"> transcranial electric field</a>, <a href="https://publications.waset.org/abstracts/search?q=electro-fenton%20effect" title=" electro-fenton effect"> electro-fenton effect</a> </p> <a href="https://publications.waset.org/abstracts/168507/transcranial-electric-field-treatments-on-redox-toxic-iron-deposits-in-transgenic-alzheimers-disease-mouse-models-the-electroceutical-targeting-of-alzheimers-disease" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/168507.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">71</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">241</span> Influence of Food Microbes on Horizontal Transfer of β-Lactam Resistance Genes between Salmonella Strains in the Mouse Gut</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Ottenbrite">M. Ottenbrite</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20Yilmaz"> G. Yilmaz</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Devenish"> J. Devenish</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Kang"> M. Kang</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Dan"> H. Dan</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Lin"> M. Lin</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Lau"> C. Lau</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Carrillo"> C. Carrillo</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Bessonov"> K. Bessonov</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Nash"> J. Nash</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Topp"> E. Topp</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Guan"> J. Guan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Consumption of food contaminated by antibiotic-resistant (AR) bacteria may lead to the transmission of AR genes in the gut microbiota and cause AR bacterial infection, a significant public health concern. However, information is limited on if and how background microbes from the food matrix (food microbes) may influence resistance transmission. Thus, we assessed the colonization of a β-lactam resistant Salmonella Heidelberg strain (donor) and a β-lactam susceptible S. Typhimurium strain (recipient) and the transfer of the resistance genes in the mouse gut in the presence or absence of food microbes that were derived from washing freshly-harvested carrots. Mice were pre-treated with streptomycin and then inoculated with both donor and recipient bacteria or recipient only. Fecal shedding of the donor, recipient, and transconjugant bacteria was enumerated using selective culture techniques. Transfer of AR genes was confirmed by whole genome sequencing. Gut microbial composition was determined by 16s rRNA amplicon sequencing. Significantly lower numbers of donors and recipients were shed from mice that were inoculated with food microbes compared to those without food microbe inoculation. S. Typhimurium transconjugants were only recovered from mice without inoculation of food microbes. A significantly higher survival rate was in mice with vs. without inoculation of food microbes. The results suggest that the food microbes may compete with both the donor and recipient Salmonella, limit their growth and reduce transmission of the β-lactam resistance gene in the mouse gut. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antibiotic%20resistance" title="antibiotic resistance">antibiotic resistance</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20transfer" title=" gene transfer"> gene transfer</a>, <a href="https://publications.waset.org/abstracts/search?q=gut%20microbiota" title=" gut microbiota"> gut microbiota</a>, <a href="https://publications.waset.org/abstracts/search?q=Salmonella%20infection" title=" Salmonella infection"> Salmonella infection</a> </p> <a href="https://publications.waset.org/abstracts/145920/influence-of-food-microbes-on-horizontal-transfer-of-v-lactam-resistance-genes-between-salmonella-strains-in-the-mouse-gut" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/145920.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">74</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">240</span> Co-Culture of Neonate Mouse Spermatogonial Stem Cells with Sertoli Cells: Inductive Role of Melatonin following Transplantation: Adult Azoospermia Mouse Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mehdi%20Abbasi">Mehdi Abbasi</a>, <a href="https://publications.waset.org/abstracts/search?q=Shadan%20Navid"> Shadan Navid</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammad%20Pourahmadi"> Mohammad Pourahmadi</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Majidi%20Zolbin"> M. Majidi Zolbin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We have recently reported that melatonin as antioxidant enhances the efficacy of colonization of spermatogonial stem cells (SSCs). Melatonin as an antioxidant plays a vital role in the development of SSCs in vitro. This study aimed to investigate evaluation of sertoli cells and melatonin simultaneously on SSC proliferation following transplantation to testis of adult mouse busulfan-treated azoospermia model. SSCs and sertoli cells were isolated from the testes of three to six-day old male mice.To determine the purity, Flow cytometry technique using PLZF antibody were evaluated. Isolated testicular cells were cultured in αMEM medium in the absence (control group) or presence (experimental group) of sertoli cells and melatonin extract for 2 weeks. We then transplanted SSCs by injection into the azoospermia mice model. Higher viability, proliferation, and Id4, Plzf, expression were observed in the presence of simultaneous sertoli cells and melatonin in vitro. Moreover, immunocytochemistry results showed higher Oct4 expression in this group. Eight weeks after transplantation, injected cells were localized at the base of seminiferous tubules in the recipient testes. The number of spermatogonia and the weight of testis were higher in the experimental group relative to control group. The results of our study suggest that this new protocol can increase the transplantation of these cells can be useful in the treatment of male infertility. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=colonization" title="colonization">colonization</a>, <a href="https://publications.waset.org/abstracts/search?q=melatonin" title=" melatonin"> melatonin</a>, <a href="https://publications.waset.org/abstracts/search?q=spermatogonial%20stem%20cell" title=" spermatogonial stem cell"> spermatogonial stem cell</a>, <a href="https://publications.waset.org/abstracts/search?q=transplantation" title=" transplantation"> transplantation</a> </p> <a href="https://publications.waset.org/abstracts/84259/co-culture-of-neonate-mouse-spermatogonial-stem-cells-with-sertoli-cells-inductive-role-of-melatonin-following-transplantation-adult-azoospermia-mouse-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/84259.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">170</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">239</span> Possible Involvement of DNA-methyltransferase and Histone Deacetylase in the Regulation of Virulence Potential of Acanthamoeba castellanii</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yi%20H.%20Wong">Yi H. Wong</a>, <a href="https://publications.waset.org/abstracts/search?q=Li%20L.%20Chan"> Li L. Chan</a>, <a href="https://publications.waset.org/abstracts/search?q=Chee%20O.%20Leong"> Chee O. Leong</a>, <a href="https://publications.waset.org/abstracts/search?q=Stephen%20Ambu"> Stephen Ambu</a>, <a href="https://publications.waset.org/abstracts/search?q=Joon%20W.%20Mak"> Joon W. Mak</a>, <a href="https://publications.waset.org/abstracts/search?q=Priyadashi%20S.%20Sahu"> Priyadashi S. Sahu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Acanthamoeba is a free-living opportunistic protist which is ubiquitously distributed in the environment. Virulent Acanthamoeba can cause fatal encephalitis in immunocompromised patients and potential blinding keratitis in immunocompetent contact lens wearers. Approximately 24 species have been identified but only the A. castellanii, A. polyphaga and A. culbertsoni are commonly associated with human infections. Until to date, the precise molecular basis for Acanthamoeba pathogenesis remains unclear. Previous studies reported that Acanthamoeba virulence can be diminished through prolonged axenic culture but revived through serial mouse passages. As no clear explanation on this reversible pathogenesis is established, hereby, we postulate that the epigenetic regulators, DNA-methyltransferases (DNMT) and histone-deacetylases (HDAC), could possibly be involved in granting the virulence plasticity of Acanthamoeba spp. Methods: Four rounds of mouse passages were conducted to revive the virulence potential of the virulence-attenuated Acanthamoeba castellanii strain (ATCC 50492). Briefly, each mouse (n=6/group) was inoculated intraperitoneally with Acanthamoebae cells (2x 105 trophozoites/mouse) and incubated for 2 months. Acanthamoebae cells were isolated from infected mouse organs by culture method and subjected to subsequent mouse passage. In vitro cytopathic, encystment and gelatinolytic assays were conducted to evaluate the virulence characteristics of Acanthamoebae isolates for each passage. PCR primers which targeted on the 2 members (DNMT1 and DNMT2) and 5 members (HDAC1 to 5) of the DNMT and HDAC gene families respectively were custom designed. Quantitative real-time PCR (qPCR) was performed to detect and quantify the relative expression of the two gene families in each Acanthamoeba isolates. Beta-tubulin of A. castellanii (Genbank accession no: XP_004353728) was included as housekeeping gene for data normalisation. PCR mixtures were also analyzed by electrophoresis for amplicons detection. All statistical analyses were performed using the paired one-tailed Student’s t test. Results: Our pathogenicity tests showed that the virulence-reactivated Acanthamoeba had a higher degree of cytopathic effect on vero cells, a better resistance to encystment challenge and a higher gelatinolytic activity which was catalysed by serine protease. qPCR assay showed that DNMT1 expression was significantly higher in the virulence-reactivated compared to the virulence-attenuated Acanthamoeba strain (p ≤ 0.01). The specificity of primers which targeted on DNMT1 was confirmed by sequence analysis of PCR amplicons, which showed a 97% similarity to the published DNA-methyltransferase gene of A. castellanii (GenBank accession no: XM_004332804.1). Out of the five primer pairs which targeted on the HDAC family genes, only HDAC4 expression was significantly difference between the two variant strains. In contrast to DNMT1, HDAC4 expression was much higher in the virulence-attenuated Acanthamoeba strain. Conclusion: Our mouse passages had successfully restored the virulence of the attenuated strain. Our findings suggested that DNA-methyltransferase (DNMT1) and histone deacetylase (HDAC4) expressions are associated with virulence potential of Acanthamoeba spp. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acanthamoeba" title="acanthamoeba">acanthamoeba</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA-methyltransferase" title=" DNA-methyltransferase"> DNA-methyltransferase</a>, <a href="https://publications.waset.org/abstracts/search?q=histone%20deacetylase" title=" histone deacetylase"> histone deacetylase</a>, <a href="https://publications.waset.org/abstracts/search?q=virulence-associated%20proteins" title=" virulence-associated proteins"> virulence-associated proteins</a> </p> <a href="https://publications.waset.org/abstracts/49185/possible-involvement-of-dna-methyltransferase-and-histone-deacetylase-in-the-regulation-of-virulence-potential-of-acanthamoeba-castellanii" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/49185.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">289</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">238</span> Kinematic Gait Analysis Is a Non-Invasive, More Objective and Earlier Measurement of Impairment in the Mdx Mouse Model of Duchenne Muscular Dystrophy </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=P.%20J.%20Sweeney">P. J. Sweeney</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20Ahtoniemi"> T. Ahtoniemi</a>, <a href="https://publications.waset.org/abstracts/search?q=J.%20Puoliv%C3%A4li"> J. Puoliväli</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20Laitinen"> T. Laitinen</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Lehtim%C3%A4ki"> K. Lehtimäki</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Nurmi"> A. Nurmi</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Wells"> D. Wells</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Duchenne muscular dystrophy (DMD) is caused by an X linked mutation in the dystrophin gene; lack of dystrophin causes a progressive muscle necrosis which leads to a progressive decrease in mobility in those suffering from the disease. The MDX mouse, a mutant mouse model which displays a frank dystrophinopathy, is currently widely employed in pre clinical efficacy models for treatments and therapies aimed at DMD. In general the end-points examined within this model have been based on invasive histopathology of muscles and serum biochemical measures like measurement of serum creatine kinase (sCK). It is established that a “critical period” between 4 and 6 weeks exists in the MDX mouse when there is extensive muscle damage that is largely sub clinical but evident with sCK measurements and histopathological staining. However, a full characterization of the MDX model remains largely incomplete especially with respect to the ability to aggravate of the muscle damage beyond the critical period. The purpose of this study was to attempt to aggravate the muscle damage in the MDX mouse and to create a wider, more readily translatable and discernible, therapeutic window for the testing of potential therapies for DMD. The study consisted of subjecting 15 male mutant MDX mice and 15 male wild-type mice to an intense chronic exercise regime that consisted of bi-weekly (two times per week) treadmill sessions over a 12 month period. Each session was 30 minutes in duration and the treadmill speed was gradually built up to 14m/min for the entire session. Baseline plasma creatine kinase (pCK), treadmill training performance and locomotor activity were measured after the “critical period” at around 10 weeks of age and again at 14 weeks of age, 6 months, 9 months and 12 months of age. In addition, kinematic gait analysis was employed using a novel analysis algorithm in order to compare changes in gait and fine motor skills in diseased exercised MDX mice compared to exercised wild type mice and non exercised MDX mice. In addition, a morphological and metabolic profile (including lipid profile), from the muscles most severely affected, the gastrocnemius muscle and the tibialis anterior muscle, was also measured at the same time intervals. Results indicate that by aggravating or exacerbating the underlying muscle damage in the MDX mouse by exercise a more pronounced and severe phenotype in comes to light and this can be picked up earlier by kinematic gait analysis. A reduction in mobility as measured by open field is not apparent at younger ages nor during the critical period, but changes in gait are apparent in the mutant MDX mice. These gait changes coincide with pronounced morphological and metabolic changes by non-invasive anatomical MRI and proton spectroscopy (1H-MRS) we have reported elsewhere. Evidence of a progressive asymmetric pathology in imaging parameters as well as in the kinematic gait analysis was found. Taken together, the data show that chronic exercise regime exacerbates the muscle damage beyond the critical period and the ability to measure through non-invasive means are important factors to consider when performing preclinical efficacy studies in the MDX mouse. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gait" title="Gait">Gait</a>, <a href="https://publications.waset.org/abstracts/search?q=muscular%20dystrophy" title=" muscular dystrophy"> muscular dystrophy</a>, <a href="https://publications.waset.org/abstracts/search?q=Kinematic%20analysis" title=" Kinematic analysis"> Kinematic analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=neuromuscular%20disease" title=" neuromuscular disease"> neuromuscular disease</a> </p> <a href="https://publications.waset.org/abstracts/23465/kinematic-gait-analysis-is-a-non-invasive-more-objective-and-earlier-measurement-of-impairment-in-the-mdx-mouse-model-of-duchenne-muscular-dystrophy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23465.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">276</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">237</span> YPFS Attenuating TH2 Cell-Mediated Allergic Inflammation by Regulating the TSLP Pathway</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Xi%20Yu">Xi Yu</a>, <a href="https://publications.waset.org/abstracts/search?q=Lili%20Gu"> Lili Gu</a>, <a href="https://publications.waset.org/abstracts/search?q=Huizhu%20Wang"> Huizhu Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Xiao%20Wei"> Xiao Wei</a>, <a href="https://publications.waset.org/abstracts/search?q=Dandan%20Sheng"> Dandan Sheng</a>, <a href="https://publications.waset.org/abstracts/search?q=Xiaoyan%20Jiang"> Xiaoyan Jiang</a>, <a href="https://publications.waset.org/abstracts/search?q=Min%20Hong"> Min Hong </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Hypersensitivity disease is difficult to cure completely because of its recurrence, yupingfengsan (YPFS) is used to treat the diseases with the advantage of reducing the recurrence,but the precise mechanism is not clear. Previous studies of our laboratory have shown that the extract of YPFS can inhibit Th2-type allergic contact dermatitis(ACD) induced by FITC.Besides, thymic stromal lymphopoietin(TSLP) have been proved to be a master switch for allergic inflammation. Based on these studies, we want to establish a mouse model of TSLP production based on Th2 cell-mediated allergic inflammation to explore the regulating mechanisms of YPFS on TSLP in Th2 cell-mediated allergic inflammation. Methods: Th2-type ACD mouse model: The mice were topically sensitized on the abdomens (induction phase) and elicited on its ears skin 6 day later (excitation phase) with FITC solution, and the ear swelling was measured to evaluate the allergic inflammation；A mouse model of TSLP production based on Th2 cell-mediated allergic inflammation (TSLP production model): the skin of the ear was sensitized on two consecutive days with FITC solution causing the production of TSLP；Mice were treated with YPFS extract，ELISA、Real-time PCR and Western-blotting were using to examine the mRNA and protein levels of TSLP\TSLPR and TLRs ect. Results: YPFS extract can attenuates Th2-type allergic inflammatory in mice；in TSLP production model, YPFS can inhibit the expression of TSLP、 TSLPR、TLRs and MyD88, So we deduce the possible mechanisms of YPFS to play a role of intervention is through TLRs- MyD88 dependent and independent pathway to reduce TSLP production. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=YPFS" title="YPFS">YPFS</a>, <a href="https://publications.waset.org/abstracts/search?q=TSLP" title=" TSLP"> TSLP</a>, <a href="https://publications.waset.org/abstracts/search?q=TLRs" title=" TLRs"> TLRs</a>, <a href="https://publications.waset.org/abstracts/search?q=Th2-type%20allergic%20contact%20dermatitis" title=" Th2-type allergic contact dermatitis"> Th2-type allergic contact dermatitis</a> </p> <a href="https://publications.waset.org/abstracts/3044/ypfs-attenuating-th2-cell-mediated-allergic-inflammation-by-regulating-the-tslp-pathway" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3044.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">422</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">236</span> Neuroprotective Effects of Rosmarinic Acid in the MPTP Mouse Model of Parkinson's Disease</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Huamin%20Xu">Huamin Xu</a>, <a href="https://publications.waset.org/abstracts/search?q=Wenting%20Jia"> Wenting Jia</a>, <a href="https://publications.waset.org/abstracts/search?q=Hong%20Jiang"> Hong Jiang</a>, <a href="https://publications.waset.org/abstracts/search?q=Junxia%20Xie"> Junxia Xie</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rosmarinic acid (RA) is a natural acid that is found in a variety of herbs, such as rosemary and has multiple biological activities such as antioxidative, anti-inflammatory and antiviral activities. In this study, we investigated the neuroprotective effects of RA on dopaminergic system in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced mouse model of Parkinson’s disease (PD). The mice received oral administration of RA before MPTP injection. Results showed that the tyrosine hydroxylase expression in SN reduced and the levels of dopamine and its metabolites in the striatum decreased in MPTP intoxicated PD mice. Pretreatment with RA significantly inhibited these changes. Further studies demonstrated that MPTP treatment increased the iron content, which was counteracted by pre-treatment with RA. In addition, RA could restore the decrease of superoxide dismutase (SOD) induced by MPTP. This study provides evidence that RA could suppress MPTP-induced degeneration of the nigrostriatal dopaminergic system by regulating iron content and the expression of SOD. Thus, RA might be clinically evaluated for the prevention of neurodegenerative diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=rosmarinic%20acid" title="rosmarinic acid">rosmarinic acid</a>, <a href="https://publications.waset.org/abstracts/search?q=Parkinson%27s%20disease" title=" Parkinson's disease"> Parkinson's disease</a>, <a href="https://publications.waset.org/abstracts/search?q=MPTP" title=" MPTP"> MPTP</a>, <a href="https://publications.waset.org/abstracts/search?q=dopaminergic%20system" title=" dopaminergic system"> dopaminergic system</a> </p> <a href="https://publications.waset.org/abstracts/74088/neuroprotective-effects-of-rosmarinic-acid-in-the-mptp-mouse-model-of-parkinsons-disease" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/74088.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">204</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">235</span> Effect of Engineered Low Glycemic Foods on Cancer Progression and Healthy State</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=C.%20Panebianco">C. Panebianco</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Adamberg"> K. Adamberg</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Adamberg"> S. Adamberg</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Saracino"> C. Saracino</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Jaagura"> M. Jaagura</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Kolk"> K. Kolk</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20Di%20Chio"> A. Di Chio</a>, <a href="https://publications.waset.org/abstracts/search?q=P.%20Graziano"> P. Graziano</a>, <a href="https://publications.waset.org/abstracts/search?q=R.%20Vilu"> R. Vilu</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20Pazienza"> V. Pazienza</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background/Aims: Despite recent advances in treatment options, a modest impact on the outcome of the pancreatic cancer (PC) is observed so far. Short-term fasting cycles have the potential to improve the efficacy of chemotherapy against PC. However, diseased people may refuse to follow the fasting regimen and fasting may worsen the weight loss often occurring in cancer patients. Therefore, alternative approaches are needed. The aim of this study was to assess the effect of Engineered Low glycemic food ELGIF mimicking diet on growth of cancer cell lines in vitro and in an in vivo pancreatic cancer mouse xenograft model. Materials and Methods: BxPC-3, MiaPaca-2 and Panc-1 cells were cultured in control and ELGIF mimicking diet culturing condition to evaluate the tumor growth and proliferation pathways. Pancreatic cancer xenograft mice were subjected to ELGIF to assess the tumor volume and weight as compared to mice fed with control diet. Results: Pancreatic cancer cells cultured in ELGIF mimicking medium showed decreased levels of proliferation as compared to those cultured in the standard medium. Consistently, xenograft pancreatic cancer mice subjected to ELGIF diet displayed a significant decrease in tumor growth. Conclusion: A positive effect of ELGIF diet on proliferation in vitro is associated with the decrease of tumor progression in the in vivo PC xenograft mouse model. These results suggest that engineered dietary interventions could be supportive as synergistic approach to enhance the efficacy of existing cancer treatments in pancreatic cancer patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=functional%20food" title="functional food">functional food</a>, <a href="https://publications.waset.org/abstracts/search?q=microbiota" title=" microbiota"> microbiota</a>, <a href="https://publications.waset.org/abstracts/search?q=mouse%20model" title=" mouse model"> mouse model</a>, <a href="https://publications.waset.org/abstracts/search?q=pancreatic%20cancer" title=" pancreatic cancer"> pancreatic cancer</a> </p> <a href="https://publications.waset.org/abstracts/51926/effect-of-engineered-low-glycemic-foods-on-cancer-progression-and-healthy-state" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/51926.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">290</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">234</span> Biodistribution Studies of 177Lu-DOTATOC in Mouse Tumor Model: Possible Utilization in Adenocarcinoma Breast Cancer Treatment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Mousavi-Daramoroudi">M. Mousavi-Daramoroudi</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20Yousefnia"> H. Yousefnia</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Abbasi-Davani"> F. Abbasi-Davani</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Zolghadri"> S. Zolghadri</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20Kakaei"> S. Kakaei</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Despite the appropriate characteristics of <sup>177</sup>Lu and DOTATOC, to our best knowledge, the therapeutic benefit of <sup>177</sup>Lu-DOTATOC complex in breast cancer has not been reported until now. In this study, biodistribution of <sup>177</sup>Lu-DOTA-TOC in mouse tumor model for evaluation of possible utilization of this complex in breast cancer treatment was investigated.<sup>177</sup>Lu was prepared with the specific activity of 2.6-3 GBq.mg<sup>-1</sup> and radionuclidic purity higher than 99%. The radiolabeled complex was prepared in the optimized conditions with the radiochemical purity higher than 99%. The final solution was injected to the BALB/c mice with adenocarcinoma breast cancer. The biodistribution results showed major accumulation in the kidneys as the major excretion route and the somatostatin receptor-positive tissues such as pancreas compared with the other tissues. Also, significant uptake was observed in tumor even in longer time after injection. According to the results obtained in this research study, somatostatin receptors expressed in breast cancers can be targeted with DOTATOC analogues especially with <sup>177</sup>Lu-DOTATOC as an ideal therapeutic agent. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=%C2%B9%E2%81%B7%E2%81%B7Lu" title="¹⁷⁷Lu">¹⁷⁷Lu</a>, <a href="https://publications.waset.org/abstracts/search?q=adenocarcinoma%20breast%20cancer" title=" adenocarcinoma breast cancer"> adenocarcinoma breast cancer</a>, <a href="https://publications.waset.org/abstracts/search?q=DOTATOC" title=" DOTATOC"> DOTATOC</a>, <a href="https://publications.waset.org/abstracts/search?q=BALB%2Fc%20mice" title=" BALB/c mice"> BALB/c mice</a> </p> <a href="https://publications.waset.org/abstracts/80226/biodistribution-studies-of-177lu-dotatoc-in-mouse-tumor-model-possible-utilization-in-adenocarcinoma-breast-cancer-treatment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/80226.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">227</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">233</span> Collagen Deposition in Lung Parenchyma Driven by Depletion of LYVE-1+ Macrophages Protects Emphysema and Loss of Airway Function</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yinebeb%20Mezgebu%20Dagnachew">Yinebeb Mezgebu Dagnachew</a>, <a href="https://publications.waset.org/abstracts/search?q=Hwee%20Ying%20Lim"> Hwee Ying Lim</a>, <a href="https://publications.waset.org/abstracts/search?q=Liao%20Wupeng"> Liao Wupeng</a>, <a href="https://publications.waset.org/abstracts/search?q=Sheau%20Yng%20Lim"> Sheau Yng Lim</a>, <a href="https://publications.waset.org/abstracts/search?q=Lim%20Sheng%20Jie%20Natalie"> Lim Sheng Jie Natalie</a>, <a href="https://publications.waset.org/abstracts/search?q=Veronique%20Angeli"> Veronique Angeli</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Collagen is essential for maintaining lung structure and function, and its remodeling has been associated with respiratory diseases, including chronic obstructive pulmonary disease (COPD). However, the cellular mechanisms driving collagen remodeling and the functional implications of this process in the pathophysiology of pulmonary diseases remain poorly understood. Using a mouse model of Lyve-1 expressing macrophage depletion, we found that the absence of this subpopulation of tissue-resident macrophage led to the preferential deposition of type I collagen fibers around the alveoli and bronchi in the steady state. Further analysis by polarized light microscopy revealed that the collagen fibers accumulating in the lungs depleted of Lyve-1+ macrophages were thicker and crosslinked. A decrease in MMP-9 gene expression and proteolytic activity, together with an increase in Col1a1, Timp-3 and Lox gene expression, accompanied the collagen alterations. Next, we investigated the effect of the collagen remodeling on the pathophysiology of COPD and airway function in mouse lacking Lyve-1+ macrophage exposed chronically to cigarette smoke (CS), a well-established animal model of COPD. We showed that the deposition of collagen protected mouse against the destruction of alveoli (emphysema) and bronchi thickening after CS exposure and prevented loss of airway function. Thus, we demonstrate that interstitial Lyve-1+ macrophages regulate the composition, amount, and architecture of the collagen network in the lungs and that such collagen remodeling functionally impacts the development of COPD. This study further supports the potential of targeting collagen as a promising approach to treating respiratory diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=lung" title="lung">lung</a>, <a href="https://publications.waset.org/abstracts/search?q=extracellular%20matrix" title=" extracellular matrix"> extracellular matrix</a>, <a href="https://publications.waset.org/abstracts/search?q=chronic%20obstructive%20pulmonary%20disease" title=" chronic obstructive pulmonary disease"> chronic obstructive pulmonary disease</a>, <a href="https://publications.waset.org/abstracts/search?q=matrix%20metalloproteinases" title=" matrix metalloproteinases"> matrix metalloproteinases</a>, <a href="https://publications.waset.org/abstracts/search?q=collagen" title=" collagen"> collagen</a> </p> <a href="https://publications.waset.org/abstracts/187346/collagen-deposition-in-lung-parenchyma-driven-by-depletion-of-lyve-1-macrophages-protects-emphysema-and-loss-of-airway-function" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/187346.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">37</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">232</span> RNA-seq Analysis of Liver from NASH-HCC Model Mouse Treated with Streptozotocin-High Fat Diet</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Bui%20Phuong%20Linh">Bui Phuong Linh</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuki%20Sakakibara"> Yuki Sakakibara</a>, <a href="https://publications.waset.org/abstracts/search?q=Ryuto%20Tanaka"> Ryuto Tanaka</a>, <a href="https://publications.waset.org/abstracts/search?q=Elizabeth%20H.%20Pigney"> Elizabeth H. Pigney</a>, <a href="https://publications.waset.org/abstracts/search?q=Taishi%20Hashiguchi"> Taishi Hashiguchi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Non-alcoholic steatohepatitis (NASH) is a chronic liver disease, often associated with type II diabetes, which sometimes progresses to more serious conditions such as liver fibrosis and hepatocellular carcinoma (HCC). NASH has become an important health problem worldwide, buttherapeutic agents for NASH have not yet been approved, and animal models with high clinical correlation are required. TheSTAM™ mouse shows the same pathological progression as human NASH patients and has been widely used for both drug efficacy and basic research, such as lipid profiling and gut microbiota research. In this study, we analyzed the RNA-seq data of STAM™mice at each pathological stage (steatosis, steatohepatitis, liver fibrosis, and HCC) and examined the clinical correlation at the genetic level. NASH was induced in male mice by a single subcutaneous injection of 200 µg streptozotocin solution 2 days after birth and feeding with high fat dietafter 4 weeks of age. The mice were sacrificed and livers collected at 6, 8, 10, 12, 16, and 20 weeks of age. For liver samples, the left lateral lobe was snap frozen in liquid nitrogen and stored at -80˚C for RNA-seq analysis. Total RNA of the cells was isolated using RNeasy mini kit. The gene expression of the canonical pathways in NASH progression from steatosis to hepatocellular carcinoma were analyzed, such as immune system process, oxidation-reduction process, lipid metabolic process. Moreover, since it has been reported that genetic traits are involved in the development of NASH-HCC, we next analyzed the genetic mutations in the STAM™mice. The number of individuals showing mutations in Mtorinvolved in Insulin signaling increases as the disease progresses, especially in the liver cancer phase. These results indicated a clinical correlation of gene profiles in the STAM™mouse. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=steatosis" title="steatosis">steatosis</a>, <a href="https://publications.waset.org/abstracts/search?q=non-alcoholic%20steatohepatitis" title=" non-alcoholic steatohepatitis"> non-alcoholic steatohepatitis</a>, <a href="https://publications.waset.org/abstracts/search?q=fibrosis" title=" fibrosis"> fibrosis</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatocellular%20carcinoma" title=" hepatocellular carcinoma"> hepatocellular carcinoma</a>, <a href="https://publications.waset.org/abstracts/search?q=RNA-seq" title=" RNA-seq"> RNA-seq</a> </p> <a href="https://publications.waset.org/abstracts/143571/rna-seq-analysis-of-liver-from-nash-hcc-model-mouse-treated-with-streptozotocin-high-fat-diet" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/143571.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">154</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">231</span> Giant Cancer Cell Formation: A Link between Cell Survival and Morphological Changes in Cancer Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rostyslav%20Horbay">Rostyslav Horbay</a>, <a href="https://publications.waset.org/abstracts/search?q=Nick%20Korolis"> Nick Korolis</a>, <a href="https://publications.waset.org/abstracts/search?q=Vahid%20Anvari"> Vahid Anvari</a>, <a href="https://publications.waset.org/abstracts/search?q=Rostyslav%20Stoika"> Rostyslav Stoika</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Giant cancer cells (GCC) are common in all types of cancer, especially after poor therapy. Some specific features of such cells include ~10-fold enlargement, drug resistance, and the ability to propagate similar daughter cells. We used murine NK/Ly lymphoma, an aggressive and fast growing lymphoma model that has already shown drastic changes in GCC comparing to parental cells (chromatin condensation, nuclear fragmentation, tighter OXPHOS/cellular respiration coupling, multidrug resistance). Materials and methods: In this study, we compared morpho-functional changes of GCC that predominantly show either a cytostatic or a cytotoxic effect after treatment with drugs. We studied the effect of a combined cytostatic/cytotoxic drug treatment to determine the correlation of drug efficiency and GCC formation. Doses of G1/S-specific drug paclitaxel/PTX (G2/M-specific, 50 mg/mouse), vinblastine/VBL (50 mg/mouse), and DNA-targeting agents doxorubicin/DOX (125 ng/mouse) and cisplatin/CP (225 ng/mouse) on C57 black mice. Several tests were chosen to estimate morphological and physiological state (propidium iodide, Rhodamine-123, DAPI, JC-1, Janus Green, Giemsa staining and other), which included cell integrity, nuclear fragmentation and chromatin condensation, mitochondrial activity, and others. A single and double factor ANOVA analysis were performed to determine correlation between the criteria of applied drugs and cytomorphological changes. Results: In all cases of treatment, several morphological changes were observed (intracellular vacuolization, membrane blebbing, and interconnected mitochondrial network). A lower gain in ascites (49.97% comparing to control group) and longest lifespan (22+9 days) after tumor injection was obtained with single VBL and single DOX injections. Such ascites contained the highest number of GCC (83.7%+9.2%), lowest cell count number (72.7+31.0 mln/ml), and a strong correlation coefficient between increased mitochondrial activity and percentage of giant NK/Ly cells. A high number of viable GCC (82.1+9.2%) was observed compared to the parental forms (15.4+11.9%) indicating that GCC are more drug resistant than the parental cells. All this indicates that the giant cell formation and its ability to obtain drug resistance is an expanding field in cancer research. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ANOVA" title="ANOVA">ANOVA</a>, <a href="https://publications.waset.org/abstracts/search?q=cisplatin" title=" cisplatin"> cisplatin</a>, <a href="https://publications.waset.org/abstracts/search?q=doxorubicin" title=" doxorubicin"> doxorubicin</a>, <a href="https://publications.waset.org/abstracts/search?q=drug%20resistance" title=" drug resistance"> drug resistance</a>, <a href="https://publications.waset.org/abstracts/search?q=giant%20cancer%20cells" title=" giant cancer cells"> giant cancer cells</a>, <a href="https://publications.waset.org/abstracts/search?q=NK%2FLy%20lymphoma" title=" NK/Ly lymphoma"> NK/Ly lymphoma</a>, <a href="https://publications.waset.org/abstracts/search?q=paclitaxel" title=" paclitaxel"> paclitaxel</a>, <a href="https://publications.waset.org/abstracts/search?q=vinblastine" title=" vinblastine"> vinblastine</a> </p> <a href="https://publications.waset.org/abstracts/56658/giant-cancer-cell-formation-a-link-between-cell-survival-and-morphological-changes-in-cancer-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56658.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">217</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">230</span> Evaluating Therapeutic Efficacy of Intravesical Xenogeneic Urothelial Cell Treatment Alone and in Combination with Chemotherapy or Immune Checkpoint Inhibitors in a Mouse Non-Muscle-Invasive Bladder Cancer Model</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chih-Rong%20Shyr">Chih-Rong Shyr</a>, <a href="https://publications.waset.org/abstracts/search?q=Chi-Ping%20Huang"> Chi-Ping Huang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Intravesical BCG is the gold-standard therapy for high risk non-muscle invasive bladder cancer (NMIBC) after TURBT, but if not responsive to BCG, these BCG unresponsive patients face cystectomy that causes morbidity and comes with a morality risk. To provide the bladder sparing options for patients with BCG-unresponsive NMIBC, several new treatments have been developed to salvage the bladders and prevent progression to muscle invasive or metastatic, but however, most approved or developed treatments still fail in a significant proportion of patients without long term success. Thus more treatment options and the combination of different therapeutic modalities are urgently needed to change the outcomes. Xenogeneic rejection has been proposed to a mechanism of action to induce anti-tumor immunity for the treatment of cancers due to the similarities between rejection mechanism to xenoantigens (proteins, glycans and lipids) and anti-tumor immunities to tumor specific antigens (neoantigens, tumor associated carbohydrates and lipids). Xenogeneic urothelial cells (XUC) of porcine origin have been shown to induce anti-tumor immune responses to inhibit bladder tumor progression in mouse bladder cancer models. To further demonstrate the efficacy of the distinct intravesical XUC treatment in NMIBC, and the combined effects with chemotherapy and immune checkpoint inhibitors (ICIs) as a alternate therapeutic option, this study investigated the therapeutic effects and mechanisms of intravesical XUC immunotherapy in an orthotopic mouse immune competent model of NMIBC, generated from a mouse bladder cancer cell line. We found that the tumor progression was inhibited by intravescial XUC treatment and there was a synergy between intravesical XUC with intravesical chemotherapeutic agent, gemcitabine or systemic ICI, anti-PD1 antibody treatment. The cancer cell proliferation was decreased but the cell death was increased by the intravecisal XUC treatment. Most importantly, the mechanisms of action of intravesical XUC immunotherapy were found to be linked to enhanced infiltration of CD4+ and CD8+ T-cell as well as NK cells, but decreased presence of myeloid immunosuppressive cells in XUC treated tumors. The increased stimulation of immune cells of XUC treated mice to xenogeneic urothelial cells and mouse bladder cancer cells in immune cell proliferation and cytokine secretion were observed both as a monotherapy and in combination with intravesical gemcitabine or systemic anti PD-L1 treatment. In sum, we identified the effects of intravesical XUC treatment in monotherapy and combined therapy on tumor progression and its cellular and molecular events related to immune activation to understand the anti-tumoral mechanisms behind intravesical XUC immunotherapy for NMIBC. These results contribute to the understanding of the mechanisms behind successful xenogeneic cell immunotherapy against NMIBC and characterize a novel therapeutic approach with a new xenogeneic cell modality for BCG-unresponsive NMIBC. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=xenoantigen" title="xenoantigen">xenoantigen</a>, <a href="https://publications.waset.org/abstracts/search?q=neoantigen" title=" neoantigen"> neoantigen</a>, <a href="https://publications.waset.org/abstracts/search?q=rejection" title=" rejection"> rejection</a>, <a href="https://publications.waset.org/abstracts/search?q=immunity" title=" immunity"> immunity</a> </p> <a href="https://publications.waset.org/abstracts/194605/evaluating-therapeutic-efficacy-of-intravesical-xenogeneic-urothelial-cell-treatment-alone-and-in-combination-with-chemotherapy-or-immune-checkpoint-inhibitors-in-a-mouse-non-muscle-invasive-bladder-cancer-model" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/194605.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">7</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">229</span> Improved Mechanical Properties and Osteogenesis in Electrospun Poly L-Lactic Ultrafine Nanofiber Scaffolds Incorporated with Graphene Oxide</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Weili%20Shao">Weili Shao</a>, <a href="https://publications.waset.org/abstracts/search?q=Qian%20Wang"> Qian Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Jianxin%20He"> Jianxin He</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Recently, the applications of graphene oxide in fabricating scaffolds for bone tissue engineering have been received extensive concern. In this work, poly l-lactic/graphene oxide composite nanofibers were successfully fabricated by electrospinning. The morphology structure, porosity and mechanical properties of the composite nanofibers were characterized using different techniques. And mouse mesenchymal stem cells were cultured on the composite nanofiber scaffolds to assess their suitability for bone tissue engineering. The results indicated that the composite nanofiber scaffolds had finer fiber diameter and higher porosity as compared with pure poly l-lactic nanofibers. Furthermore, incorporation of graphene oxide into the poly l-lactic nanofibers increased protein adsorptivity, boosted the Young’s modulus and tensile strength by nearly 4.2-fold and 3.5-fold, respectively, and significantly enhanced adhesion, proliferation, and osteogenesis in mouse mesenchymal stem cells. The results indicate that composite nanofibers could be excellent and versatile scaffolds for bone tissue engineering. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=poly%20l-lactic" title="poly l-lactic">poly l-lactic</a>, <a href="https://publications.waset.org/abstracts/search?q=graphene%20oxide" title=" graphene oxide"> graphene oxide</a>, <a href="https://publications.waset.org/abstracts/search?q=osteogenesis" title=" osteogenesis"> osteogenesis</a>, <a href="https://publications.waset.org/abstracts/search?q=bone%20tissue%20engineering" title=" bone tissue engineering"> bone tissue engineering</a> </p> <a href="https://publications.waset.org/abstracts/67896/improved-mechanical-properties-and-osteogenesis-in-electrospun-poly-l-lactic-ultrafine-nanofiber-scaffolds-incorporated-with-graphene-oxide" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/67896.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">306</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">228</span> Suppression of DMBA/TPA-Induced Skin Tumorigenesis by Menthol through Inhibition of Inflammation, NF-kappaB, Ras-Raf-ERK Pathway</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zhaoguo%20Liu">Zhaoguo Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Cunsi%20Shen"> Cunsi Shen</a>, <a href="https://publications.waset.org/abstracts/search?q=Yin%20Lu"> Yin Lu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Growing evidence has shown that menthol has potent anticancer activity in various human cancers. However, its effect on skin cancer remains largely unknown. In the present study, we investigated the chemopreventive potential of menthol against 7, 12-dimethylbenz[a] anthracene(DMBA)/12-O-tetradecanoylphorbol 13-acetate (TPA)-induced skin tumorigenesis in ICR mice. Our results showed that menthol significantly inhibited TPA-induced inflammatory responses and pro-inflammatory cytokine release. We also found that menthol treatment significantly inhibited TPA-induced lipid peroxidation (LPO), mouse UDP-glucumno-syltransferase (UGT), mouse NADH Dehydrogenase, Quinone 1 (NQO1) release. Furthermore, we found menthol treatment significantly inhibited the tumor incidence and number of tumors (P < 0.001). Interestingly, we observed that menthol treatment significantly inhibited TPA-induced altered activity of NF-κB in skin tumor. Consistently, menthol-treated tumors also showed significantly suppressed the Ras-Raf-ERK signaling pathway. Thus, our results suggest that menthol inhibits DMBA/TPA-induced skin tumorigenesis by attenuating the Ras and inhibiting NF-κB activity via inhibition of inflammation responses and pro-inflammatory cytokine release. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=DMBA%2FTPA" title="DMBA/TPA">DMBA/TPA</a>, <a href="https://publications.waset.org/abstracts/search?q=NF-%CE%BAB" title=" NF-κB"> NF-κB</a>, <a href="https://publications.waset.org/abstracts/search?q=Ras-Raf-ERK" title=" Ras-Raf-ERK"> Ras-Raf-ERK</a>, <a href="https://publications.waset.org/abstracts/search?q=skin%20tumorigenesis" title=" skin tumorigenesis "> skin tumorigenesis </a> </p> <a href="https://publications.waset.org/abstracts/2813/suppression-of-dmbatpa-induced-skin-tumorigenesis-by-menthol-through-inhibition-of-inflammation-nf-kappab-ras-raf-erk-pathway" class="btn btn-primary btn-sm">Procedia</a> <a 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