CINXE.COM

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class="ds_title">Human Phospho-CrkL (Y207) Antibody </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: MAB6910</span> <a href="/reviews/MAB6910" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="product_ordering_wrapper col-xs-12 col-md-8 col-md-push-4"> <div class="product_ordering"> <form action="/products/human-phospho-crkl-y207-antibody-668915_mab6910" method="post" id="atc-datasheet-form" accept-charset="UTF-8"><div><div id="atc_datasheet_block">     <table class="add_to_cart"> <thead> <tr> <th class="ds_catalog">Catalog #</th> <th class="ds_availability_column mobile-hide">Availability</th> <th class="ds_size_price_column">Size / Price</th> <th class="ds_quantity_column">Qty</th> </tr> </thead> <tbody> <tr class="et_atc_product" data-sku="MAB6910"> <td class="dat_catalog_column">MAB6910</td> <td class="et_atc_results" colspan="2"></td> <td class="ds_quantity ds_quantity_column"><div class="form-item form-item-catnum-mab6910 form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-mab6910" name="catnum-MAB6910" value="" size="5" maxlength="3" /></div></td> </tr> <tr class="et_atc_product" data-sku="MAB6910-SP"> <td class="dat_catalog_column">MAB6910-SP</td> <td class="et_atc_results" colspan="2"></td> <td class="ds_quantity ds_quantity_column"><div class="form-item form-item-catnum-mab6910-sp form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-mab6910-sp" name="catnum-MAB6910-SP" value="" size="5" maxlength="3" /></div></td> </tr> </tbody> </table> <div class="ds_atc_pre_buttons"></div> <div class="ds_atc_buttons"> <a href="/quote/instruments/ella-automated-immunoassay-system" class="btn btn-secondary atc_request_quote_simple_plex_assay" style="display:none" rel="nofollow">Request a Quote</a> <a href="/services/bulk-quote-request?catnum=MAB6910&product=Human Phospho-CrkL (Y207) Antibody" class="btn btn-secondary atc_hidden bulk_btn" style="display:none;" rel="nofollow" title="Competitive discounts on products purchased in bulk quantities">Bulk Orders</a> <button class="btn btn-primary atc_hidden atc_btn form-submit icon-before" style="display:none;" title="Enter quantities above and click to add to cart" type="submit" id="edit-add-to-cart" name="add_to_cart_btn" value="Add to Cart"><span class="icon glyphicon glyphicon-plus" aria-hidden="true"></span> Add to Cart</button> </div> <div class="ds_atc_post_buttons"></div> </div> <input type="hidden" name="form_build_id" value="form-MqwD9T7Qj8BUWDxwSQ415C-gYw-7VH5hG4NFQffID4Y" /> <input type="hidden" name="form_id" value="atc_datasheet_form" /> </div></form> </div> </div> <div class="product_image_wrapper col-xs-12 col-md-4 col-md-pull-8"> <div class="product_image"> <span class="product_image_label"> </span> <a class="ds-image overview-images" data-toggle="lightbox" data-gallery="overview-images" onClick="event.preventDefault();" data-title="Detection of Human Phospho-CrkL (Y207) by Western Blot." data-footer="Detection of Human Phospho-CrkL (Y207) by Western Blot." title="Cat. No. MAB6910 - Human Phospho-CrkL (Y207) Antibody" href="https://resources.rndsystems.com/images/datasheets/antibody/CrkL_MAB6910_Western_Blot_11267.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/images/datasheets/antibody/CrkL_MAB6910_Western_Blot_11267.jpg" class="img-fluid" alt="Detection of Human Phospho-CrkL (Y207) by Western Blot." title="Detection of Human Phospho-CrkL (Y207) by Western Blot."> </a> <div class="search_image_other_count"> <span class="search_more_images">1 Image</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/crkl?category=Primary Antibodies" class="btn btn-secondary">All CrkL Antibodies</a> </div> </div> <div class="tabbed_content" id="rnd_antibody"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_preparation_storage" id="product-anchor-ds_preparation_storage">Preparation and Storage</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_calculator" id="product-anchor-ds_calculator">Reconstitution Calculator</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_related_research_areas" id="product-anchor-ds_related_research_areas">Related Research Areas</a> </div> <h2 id="ds_summary">Human Phospho-CrkL (Y207) Antibody Summary</h2> <div class="datasheet_section"> <div class="ds_table summary_table"> <div class="ds_table_body">  <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Species Reactivity</div> <div class="ds_table_cell summary_table_data"> Human </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Specificity</div> <div class="ds_table_cell summary_table_data"> Detects human Phospho-CrkL (Y207) in direct ELISAs and Western blots. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Source</div> <div class="ds_table_cell summary_table_data"> Monoclonal Mouse IgG<sub>2B</sub> Clone # 668915 </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Purification</div> <div class="ds_table_cell summary_table_data"> Protein A or G purified from hybridoma culture supernatant </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Immunogen</div> <div class="ds_table_cell summary_table_data"> Phosphopeptide containing the human CrkL Y207 site </div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Formulation</div> <div class="ds_table_cell summary_table_data"> Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. </div> </div> </div> </div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="MAB6910"></div> <div id="ds_applications" class="datasheet_section"> <h2>Applications</h2> <div class="ds_table app_table"> <div class="ds_table_head"> <div class="ds_table_head_row"> <div class="ds_table_head_cell app_table_blank"></div> <div class="ds_table_head_cell app_table_rec_conc">Recommended Concentration</div> <div class="ds_table_head_cell app_table_sample">Sample</div> </div> </div> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell app_table_name">Western Blot</div> <div class="ds_table_cell app_table_dilution">0.5 µg/mL</div> <div class="ds_table_cell app_table_sample">See below</div> </div> </div> </div> <p>Please Note: Optimal dilutions should be determined by each laboratory for each application. <a href="/resources/protocols-troubleshooting-guides">General Protocols</a> are available in the Technical Information section on our website. </p> </div> <div id="ds_scientific_data" class="datasheet_section"> <div id="ds_data_examples"> <h2>Scientific Data</h2> <div id="data_examples"> <div class="row data_ex_row"> <a id="ds_image_0" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> Western Blot </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-0" data-title="Detection of Human Phospho-CrkL (Y207) by Western Blot." data-footer="Detection of Human Phospho-CrkL (Y207) antibody by Western Blot." title="Cat. No. MAB6910 - Detection of Human Phospho-CrkL (Y207) antibody by Western Blot." href="https://resources.rndsystems.com/images/datasheets/antibody/CrkL_MAB6910_Western_Blot_11267.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/antibody/CrkL_MAB6910_Western_Blot_11267.jpg" class="img-fluid" alt="Detection of Human Phospho-CrkL (Y207) antibody by Western Blot." title="Detection of Human Phospho-CrkL (Y207) antibody by Western Blot."> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Detection of Human Phospho-CrkL (Y207) by Western Blot.</span> Western blot shows lysates of K562 human chronic myelogenous leukemia cell line untreated (-) or treated (+) with 300 U/mL CIP for 60 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-CrkL (Y207) Monoclonal Antibody (Catalog # MAB6910) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class="NoLineLink" href="https://www.rndsystems.com/products/mouse-igg-horseradish-peroxidase-conjugated-antibody_haf007">HAF007</a>). A specific band was detected for Phospho-CrkL (Y207) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using <a class=NoLineLink href="https://www.rndsystems.com/resources/technical/western-blot-conditions">Immunoblot Buffer Group 1</A>. </p> </div> </div> </div> </div> </div> <div id="ds_calculator" class="datasheet_section"> <div id="btn_calculator_wrapper"><div class="datasheet_calculator_toggle btn btn-secondary" id="btn_reconstitution">Reconstitution Calculator</div></div><div id="datasheet_calculators"><div id="btn_reconstitution_calc" class="datasheet_calculator"><h2>Reconstitution Calculator</h2><div id="reconstitution_calculator" class="calculator"> <form id="recCalc"> <div class="reconstitution_calculator_text" id="reconstitution_calculator_instructions"> <p>The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.</p> </div> <div class="calc_section"> <label for="reconstitution_calculator_volume" class="calculator_label">Volume (to add to vial)</label> <input id="reconstitution_calculator_volume" name="reconstitution_calculator_volume" type="text" maxlength="16" placeholder="volume" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_volume_unit" class="calculator_input calculator_select"> <option value="ml">ml</option> <option value="ul" selected="selected">ul</option> </select> </span> </div> <span class="calculator_equals calculator_operator">=</span> <div class="calc_section"> <label for="reconstitution_calculator_mass" class="calculator_label">Mass (in vial)</label> <input id="reconstitution_calculator_mass" name="reconstitution_calculator_mass" type="text" maxlength="16" placeholder="mass" class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_mass_unit" class="calculator_input calculator_select"> <option value="mg">mg</option> <option value="ug" selected="selected">ug</option> <option value="ng">ng</option> </select> </span> </div> <span class="calculator_divide calculator_operator">÷</span> <div class="calc_section"> <label for="reconstitution_calculator_concentration" class="calculator_label">Desired Reconstitution Concentration</label> <input id="reconstitution_calculator_concentration" name="reconstitution_calculator_concentration" type="text" maxlength="16" placeholder="conc." class="calculator_input calculator_text"/> <span class="select_wrapper"> <select id="reconstitution_calculator_concentration_unit" class="calculator_input calculator_select"> <option value="mg/ml">mg/ml</option> <option value="ug/ml" selected="selected">ug/ml</option> <option value="ng/ml">ng/ml</option> </select> </span> </div> <div id="reconstitution_foot" class="calc_section"> <input type="submit" value="Calculate" id="button_reconstitution_calculator_calculate" class="calculator_button btn btn-secondary"/> </div> </form> </div> </div></div> </div> <div id="ds_preparation_storage" class="datasheet_section"> <h2>Preparation and Storage</h2> <div class="ds_table prep_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Reconstitution</div> <div class="ds_table_cell prep_table_data">Sterile PBS to a final concentration of 0.5 mg/mL. <div id="reconstitution-buffer"> <div id="reconstitution-buffer-placeholder"> <div class="et_spinner_small"></div> Loading... </div> </div> </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Shipping</div> <div class="ds_table_cell prep_table_data">Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Stability & Storage</div> <div class="ds_table_cell prep_table_data"><b>Use a manual defrost freezer and avoid repeated freeze-thaw cycles. </b><UL><LI>12 months from date of receipt, -20 to -70 °C as supplied.</LI><LI>1 month, 2 to 8 °C under sterile conditions after reconstitution.</LI><LI>6 months, -20 to -70 °C under sterile conditions after reconstitution.</LI></UL> </div> </div> </div> </div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: CrkL</h2> <p>CrkL is a widely-expressed 39 kDa adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoietic origin. Through its SH2 and SH3 domains, CrkL initiates interactions with numerous downstream proteins such as CBL, p130Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. CrkL is involved in such processes as adhesion, migration and the immune response. Furthermore, this adaptor protein plays an essential role in Chronic Myelogenous Leukemia (CML) and is constitutively phosphorylated at Y207 in CML neutrophils by the BCR/Abl kinase.</p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Long Name</div> <div class="ds_table_cell background_table_data">V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Entrez Gene IDs</div> <div class="ds_table_cell background_table_data">1399 (Human); 12929 (Mouse); 287942 (Rat)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names</div> <div class="ds_table_cell background_table_data">CrkL; crk-like protein; pCRKL; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog (avian)-like</div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/mab6910.pdf?v=20241123" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/human-phospho-crkl-y207-antibody-668915_mab6910">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//aero.bio-techne.com/sds/pdf/mab6910?v=20241123">SDS</a> </div> </div> </div> <div id="ds_related_research_areas" class="datasheet_section"> <h2>Related Research Areas</h2> <ul class="ds_details"> <li> <a href="/research-area/actin-stress-fibers-and-focal-adhesions-during-emt">Actin Stress Fibers and Focal Adhesions During EMT</a> </li> <li> <a href="/research-area/adaptor-proteins">Adaptor Proteins</a> </li> <li> <a href="/research-area/adaptor-proteins-in-the-akt-pathway">Adaptor Proteins in the Akt Pathway</a> </li> <li> <a href="/research-area/apoptosis-adaptor-proteins">Apoptosis Adaptor Proteins</a> </li> <li> <a href="/research-area/focal-adhesions">Focal Adhesions</a> </li> <li> <a href="/research-area/interferons">Interferons</a> </li> </ul> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/primary-antibodies"> View all Antibody FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="mab6910" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for Human Phospho-CrkL (Y207) Antibody</h3> <div class="row"> <div class="col-xs-12"> <div> <div class="row"> <div class="col-md-6"> <p>There are currently no reviews for this product. 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