Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

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usa-input-error-message selection-validation-message"> Name must be less than 100 characters </div> </div> <div class="action-panel-control-wrap existing-collections-controls"> <label for="collections-action-panel-add-to-existing" class="action-panel-label"> Choose a collection: </label> <select id="collections-action-panel-add-to-existing" class="action-panel-selector collections-action-add-to-existing" data-ga-category="save_share" data-ga-action="select_collection" data-ga-label="($('#collections-action-add-to-existing').val() === 'Favorites') ? 'Favorites' : 'non_favorites_collection'"> </select> <div class="collections-retry-load-on-error usa-input-error-message selection-validation-message"> Unable to load your collection due to an error<br> <a href="#">Please try again</a> </div> </div> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Adding..." data-pinger-ignore data-ga-category="save_share" data-ga-action="collections" data-ga-label="add"> Add </button> <button class="action-panel-cancel" aria-label="Close 'Add to Collections' panel" ref="linksrc=close_collections_panel" aria-controls="collections-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="collections" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="bibliography-action-panel" class="bibliography-action-panel action-panel in-progress-dots-panel" aria-hidden="true" data-bibliography-open-panel-enabled="false" data-bibliography-open-panel-url-hash="#open-bibliography-panel"> <div class="inner-wrap"> <h3 class="action-panel-heading"> Add to My Bibliography </h3> <form id="bibliography-action-panel-form" class="bibliography-action-panel-form action-panel-content action-form action-panel-smaller-selectors" data-add-to-bibliography-max-amount="100" data-add-to-bibliography-batch-size="10" data-bibliography-delegates-url="/list-bibliography-delegates/" data-add-to-bibliography-url="/add-to-bibliography/" data-get-article-ids-by-search-url="/get-article-ids-by-search/" data-mybib-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/mybibliography/"> <input type="hidden" name="csrfmiddlewaretoken" value="8nXCWkIVF40WX1J0hyu5EsSIYP4HJESTLCF7sOfXvtvQfCZYWo0UAWeFVM4ogGGR"> <div class="action-panel-control-wrap bibliographies-controls"> <div class="choice-group"> <ul class="bibliographies-action-add radio-group-items"> <li> <input name="bibliography" id="my-bibliography" class="my-bibliography" type="radio" checked/> <label for="my-bibliography">My Bibliography</label> </li> </ul> </div> </div> <div class="bibliographies-retry-load-on-error usa-input-error-message selection-validation-message"> Unable to load your delegates due to an error<br> <a href="#">Please try again</a> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Adding..." data-pinger-ignore> Add </button> <button class="action-panel-cancel" aria-label="Close 'Add to bibliography' panel" ref="linksrc=close_bibliography_panel" aria-controls="bibliography-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="mybib" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="saved-search-action-panel" class="saved-search-action-panel action-panel " aria-hidden="true" data-saved-search-open-panel-enabled="false" data-saved-search-open-panel-url-hash="#open-saved-search-panel"> <div class="inner-wrap"> <h2 class="action-panel-heading"> Your saved search </h2> <form id="saved-search-action-panel-form" class="saved-search-action-panel-form action-panel-content action-form" data-create-saved-search-url="/create-saved-search/" data-try-search-terms-url="/try-search-term/" data-saved-search-root-url="https://www.ncbi.nlm.nih.gov/myncbi/searches/"> <input type="hidden" name="csrfmiddlewaretoken" value="8nXCWkIVF40WX1J0hyu5EsSIYP4HJESTLCF7sOfXvtvQfCZYWo0UAWeFVM4ogGGR"> <div class="action-panel-control-wrap"> <label for="saved-search-name" class="action-panel-label saved-search-name-label required-field-asterisk"> Name of saved search: </label> <input maxlength="200" type="text" name="saved-search-name" id="saved-search-name" class="saved-search-name" value="" required pattern="[^"&=<>\/]*" title="The following characters are not allowed in the Name field: "&=<>/"> </div> <div class="action-panel-control-wrap"> <label for="saved-search-term" class="action-panel-label required-field-asterisk"> Search terms: </label> <textarea name="saved-search-term" id="saved-search-term" class="saved-search-term" required></textarea> </div> <div class="test-search-term-wrap"> <a href="#" class="try-search-term">Test search terms</a> </div> <div class="choice-group action-panel-extra-margin-top"> <span class="action-panel-label" id="fieldset-label"> Would you like email updates of new search results? </span> <fieldset id="saved-search-alert" aria-describedby="fieldset-label"> <legend class="usa-sr-only">Saved Search Alert Radio Buttons</legend> <ul class="radio-group-items"> <li> <input type="radio" id="saved-search-alert-yes" class="saved-search-alert-yes" name="saved-search-alert" value="yes" checked> <label for="saved-search-alert-yes" class="action-panel-label">Yes</label> </li> <li> <input aria-label="No radio input" type="radio" id="saved-search-alert-no" class="saved-search-alert-no" name="saved-search-alert" value="no"> <label for="saved-search-alert-no" class="action-panel-label">No</label> </li> </ul> </fieldset> </div> <div class="alert-schedule-wrap"> <div class="action-panel-control-wrap"> <label class="action-panel-label"> Email: </label> <span aria-label="Email address" id="saved-search-email" class="action-panel-label"><span class="action-panel-label-bold"></span> (<a class="myncbi-account-settings" href="https://www.ncbi.nlm.nih.gov/account/settings/">change</a>)</span> </div> <div class="action-panel-control-wrap action-panel-extra-margin-top"> <label for="saved-search-frequency" class="action-panel-label"> Frequency: </label> <select id="saved-search-frequency" class="no-border-panel-selector saved-search-frequency"> <option value="monthly">Monthly</option> <option value="weekly">Weekly</option> <option value="daily">Daily</option> </select> </div> <div class="action-panel-control-wrap saved-search-monthly-additional"> <label for="saved-search-monthly-on-day" class="action-panel-label"> Which day? </label> <select id="saved-search-monthly-on-day" class="no-border-panel-selector"> <option value="Sunday">The first Sunday</option> <option value="Monday">The first Monday</option> <option value="Tuesday">The first Tuesday</option> <option value="Wednesday">The first Wednesday</option> <option value="Thursday">The first Thursday</option> <option value="Friday">The first Friday</option> <option value="Saturday">The first Saturday</option> <option value="day">The first day</option> <option value="weekday">The first weekday</option> </select> </div> <div class="action-panel-control-wrap saved-search-weekly-additional"> <label for="saved-search-weekly-on-day" class="action-panel-label"> Which day? </label> <select id="saved-search-weekly-on-day" class="no-border-panel-selector saved-search-weekly-on-day"> <option value="Sunday">Sunday</option> <option value="Monday">Monday</option> <option value="Tuesday">Tuesday</option> <option value="Wednesday">Wednesday</option> <option value="Thursday">Thursday</option> <option value="Friday">Friday</option> <option value="Saturday">Saturday</option> </select> </div> <div class="action-panel-control-wrap"> <label for="saved-search-report" class="action-panel-label"> Report format: </label> <select id="saved-search-report" class="no-border-panel-selector saved-search-report"> <option value="DocSum">Summary</option> <option value="DocSumText">Summary (text)</option> <option value="Abstract">Abstract</option> <option value="AbstractText">Abstract (text)</option> <option value="MEDLINE">PubMed</option> </select> </div> <div class="action-panel-control-wrap"> <label for="saved-search-amount" class="action-panel-label"> Send at most: </label> <select id="saved-search-amount" class="no-border-panel-selector saved-search-amount"> <option value="1">1 item</option> <option value="5" selected>5 items</option> <option value="10">10 items</option> <option value="20">20 items</option> <option value="50">50 items</option> <option value="100">100 items</option> <option value="200">200 items</option> </select> </div> <div> <input type="checkbox" id="saved-search-send-if-no-result" class="saved-search-send-if-no-result" name="saved-search-send-if-no-result"> <label for="saved-search-send-if-no-result" class="action-panel-label smaller-checkbox"> Send even when there aren't any new results </label> </div> <div class="action-panel-control-wrap option-text-in-email-wrap"> <label for="saved-search-email-text" class="action-panel-label"> Optional text in email: </label> <textarea name="saved-search-email-text" id="saved-search-email-text" class="saved-search-email-text"></textarea> </div> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Saving..." data-ga-category="save_share" data-ga-action="alert" data-ga-label="save"> Save </button> <button class="action-panel-cancel" aria-label="Close 'Your saved search' panel" ref="linksrc=close_saved_search_panel" aria-controls="saved-search-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="alert" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="citation-manager-action-panel" class="citation-manager-action-panel action-panel" aria-hidden="true"> <div class="inner-wrap"> <h2 class="action-panel-heading"> Create a file for external citation management software </h2> <form id="citation-manager-action-panel-form" class="action-panel-content action-form" action="/results-export-ids/" data-by-search-action="/results-export-search-data/" data-by-ids-action="/results-export-ids/" method="post" data-by-search-method="post" data-by-ids-method="post"> <input type="hidden" name="csrfmiddlewaretoken" value="8nXCWkIVF40WX1J0hyu5EsSIYP4HJESTLCF7sOfXvtvQfCZYWo0UAWeFVM4ogGGR"> <input name="results-format" type="hidden" value="pubmed"/> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Sending..." data-ga-category="save_share" data-ga-action="citation_manager" data-ga-label="save"> Create file </button> <button class="action-panel-cancel" aria-label="Close 'Send citations to citation manager' panel" ref="linksrc=close_citation_manager_panel" aria-controls="citation-manager-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="citation_manager" data-ga-label="cancel"> Cancel </button> </div> </form> </div> </div> <div id="rss-action-panel" class="rss-action-panel action-panel " aria-hidden="true"> <div class="inner-wrap"> <h2 class="action-panel-heading"> Your RSS Feed </h2> <form id="rss-action-panel-form" class="rss-action-panel-form action-panel-content action-form" data-create-rss-feed-url="/create-rss-feed-url/" data-search-form-term-value=""> <input type="hidden" name="csrfmiddlewaretoken" value="8nXCWkIVF40WX1J0hyu5EsSIYP4HJESTLCF7sOfXvtvQfCZYWo0UAWeFVM4ogGGR"> <div class="action-panel-control-wrap"> <label for="rss-name" class="action-panel-label required-field-asterisk"> Name of RSS Feed: </label> <input maxlength="200" placeholder="Name" type="text" name="rss-name" id="rss-name" class="rss-name" value='' required pattern="[^"&=<>\/]*" title="The following characters are not allowed in the Name field: "&=<>/"> </div> <div class="rss-limit-wrap"> <div class="action-panel-control-wrap action-panel-extra-margin-top"> <label for="rss-limit" class="action-panel-label"> Number of items displayed: </label> <select id="rss-limit" class="no-border-panel-selector rss-limit"> <option value="5">5</option> <option value="10">10</option> <option value="15" selected="selected">15</option> <option value="20">20</option> <option value="50">50</option> <option value="100">100</option> </select> </div> </div> <div class="action-panel-actions"> <button class="action-panel-submit" type="submit" data-loading-label="Creating..." data-ga-category="save_share" data-ga-action="alert" data-ga-label="save"> Create RSS </button> <button class="action-panel-cancel" aria-label="Close 'Your RSS' panel" ref="linksrc=close_rss_panel" aria-controls="rss-action-panel" aria-expanded="false" data-ga-category="save_share" data-ga-action="alert" data-ga-label="cancel"> Cancel </button> </div> <div class="action-panel-control-wrap rss-link-copy-wrap"> <label for="rss-link" class="usa-sr-only">RSS Link</label> <input placeholder="Your RSS Feed Link" type="text" name="rss-link" id="rss-link" class="rss-link" title="RSS Link"> <button type="button" disabled class="rss-link-copy-button disabled" data-ga-category="save_share" data-ga-action="rss" data-ga-label="copy"> Copy </button> </div> </form> </div> </div> </div> </div> <div class="article-page" id="article-page" data-article-pmid="30550275"> <aside class="page-sidebar"> <div class="inner-wrap"> <div class="full-text-links"> <div class="full-view"> <h3 class="title"> Full text links </h3> <div class="full-text-links-list"> <a class="link-item dialog-focus" 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required-field-asterisk"> Name your collection: </label><input type="text" name="add-to-new-collection" id="collections-action-dialog-add-to-new" class="collections-action-add-to-new" pattern="[^"&=<>\/]*" title="The following characters are not allowed in the Name field: "&=<>/" maxlength="100" data-ga-category="collections_button" data-ga-action="create_collection" data-ga-label="non_favorties_collection"><div class="collections-new-name-too-long usa-input-error-message selection-validation-message"> Name must be less than 100 characters </div></div><div class="action-panel-control-wrap existing-collections-controls"><label for="collections-action-dialog-add-to-existing" class="action-panel-label"> Choose a collection: </label><select id="collections-action-dialog-add-to-existing" class="action-panel-selector collections-action-add-to-existing" data-ga-category="collections_button" data-ga-action="select_collection" data-ga-label="($('.collections-action-add-to-existing').val() === 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'Favorites' : 'non_favorites_collection'"></select><div class="collections-retry-load-on-error usa-input-error-message selection-validation-message"> Unable to load your collection due to an error<br><a href="#">Please try again</a></div></div></div><div class="action-panel-actions"><button class="action-panel-submit" type="submit" data-loading-label="Adding..." data-pinger-ignore data-ga-category="collections_button" data-ga-action="click" data-ga-label="add"> Add </button><button class="action-panel-cancel" aria-label="Close 'Add to Collections' panel" ref="linksrc=close_collections_panel" aria-controls="collections-action-panel" aria-expanded="false" data-ga-category="collections_button" data-ga-action="click" data-ga-label="cancel"> Cancel </button></div></form></div></div></div></div><button class="more-actions-button more-actions-dialog-trigger" title="Open dialog with more actions to take" ref="linksrc=more_actions_btn"></button><div class="display-options" 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<div class="authors"> <h3 class="title"> Authors </h3> <div class="authors-list"> <span class="authors-list-item "><a class="full-name" href="/?term=Greenwald+EC&cauthor_id=30550275" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Eric C Greenwald">Eric C Greenwald</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="University of California , San Diego, 9500 Gilman Drive, BRFII , La Jolla , CA 92093-0702 , United States." href="#short-view-affiliation-1" ref="linksrc=author_aff"> 1 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Mehta+S&cauthor_id=30550275" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Sohum Mehta">Sohum Mehta</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="University of California , San Diego, 9500 Gilman Drive, BRFII , La Jolla , CA 92093-0702 , United States." href="#short-view-affiliation-1" ref="linksrc=author_aff"> 1 </a></sup><span class="comma">, </span></span><span class="authors-list-item "><a class="full-name" href="/?term=Zhang+J&cauthor_id=30550275" ref="linksrc=author_name_link" data-ga-category="search" data-ga-action="author_link" data-ga-label="Jin Zhang">Jin Zhang</a><sup class="affiliation-links"><span class="author-sup-separator"> </span><a class="affiliation-link" title="University of California , San Diego, 9500 Gilman Drive, BRFII , La Jolla , CA 92093-0702 , United States." href="#short-view-affiliation-1" ref="linksrc=author_aff"> 1 </a></sup></span> </div> </div> <div class="affiliations"> <h3 class="title"> Affiliation </h3> <ul class="item-list"> <li data-affiliation-id="short-view-affiliation-1" id="short-view-affiliation-1" ><sup class="key">1</sup> University of California , San Diego, 9500 Gilman Drive, BRFII , La Jolla , CA 92093-0702 , United States.</li> </ul> </div> </div> <ul class="identifiers" id="short-view-identifiers"> <li> <span class="identifier pubmed"> <span class="id-label"> PMID: </span> <strong class="current-id" title="PubMed ID">30550275</strong> </span> </li> <li> <span class="identifier pmc"> <span class="id-label"> PMCID: </span> <a class="id-link" target="_blank" rel="noopener" ref="linksrc=article_id_link&article_id=PMC7462118&id_type=PMC" href="https://pmc.ncbi.nlm.nih.gov/articles/PMC7462118/" data-ga-category="full_text" data-ga-action="PMCID" > PMC7462118 </a> </span> </li> <li> <span class="identifier doi"> <span class="id-label"> DOI: </span> <a class="id-link" target="_blank" rel="noopener" ref="linksrc=article_id_link&article_id=10.1021/acs.chemrev.8b00333&id_type=DOI" href="https://doi.org/10.1021/acs.chemrev.8b00333" data-ga-category="full_text" data-ga-action="DOI" > 10.1021/acs.chemrev.8b00333 </a> </span> </li> </ul> <div class="in-clipboard-label " hidden> Item in Clipboard </div> </div> </div> </header> <div class="actions-buttons inline"><div class="inner-wrap"><button class="full-text-links-button full-text-links-dialog-trigger" title="Open dialog with full text links" ref="linksrc=full_text_links"> Full text links </button><button class="citation-button citation-dialog-trigger" aria-label="Open dialog with citation text in different styles" data-ga-category="save_share" data-ga-action="cite" data-ga-label="open" data-all-citations-url="/30550275/citations/" data-citation-style="nlm" data-pubmed-format-link="/30550275/export/"><span class="button-label">Cite</span></button><button class="more-actions-button more-actions-dialog-trigger" title="Open dialog with more actions to take" ref="linksrc=more_actions_btn"></button><div class="display-options" data-format-key="format" data-default-format-value="abstract" data-current-format-value="abstract" role="region"><button class="trigger usa-button" title="Change format" data-ga-category="display_options" data-ga-action="display_options_dialog"><span class="button-label">Display options</span></button><div class="dropdown" hidden><div class="title"> Display options </div><div class="content"><div class="format-container"><span class="option-label"> Format </span><select name="format" data-initial-value="abstract" aria-label="Display format" id="inline-actions-display-format"><option value="abstract">Abstract</option><option value="pubmed">PubMed</option><option value="pmid">PMID</option></select></div></div></div></div></div></div> <div class="abstract" id="abstract"> <h2 class="title"> Abstract </h2> <div class="abstract-content selected" id="eng-abstract"> <p> Cellular signaling networks are the foundation which determines the fate and function of cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over 25 years ago enabled the development of a diverse array of genetically encodable fluorescent biosensors that are capable of measuring the spatiotemporal dynamics of signal transduction pathways in live cells. In an effort to encapsulate the breadth over which fluorescent biosensors have expanded, we endeavored to assemble a comprehensive list of published engineered biosensors, and we discuss many of the molecular designs utilized in their development. Then, we review how the high temporal and spatial resolution afforded by fluorescent biosensors has aided our understanding of the spatiotemporal regulation of signaling networks at the cellular and subcellular level. Finally, we highlight some emerging areas of research in both biosensor design and applications that are on the forefront of biosensor development. </p> </div> </div> <p class="disclaimer" id="disclaimer"> <a href="/disclaimer/" target="_blank" data-ga-category="literature_resources" data-ga-action="disclaimer_link" data-ga-label="under_abstract">PubMed Disclaimer</a> </p> <div class="figures" id="figures"> <h2 class="title"> Figures </h2> <div class="figures-list" id="slides-container" itemscope itemtype="http://schema.org/ImageGallery"> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="0" data-label-slug="figure-1"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/d6b546aae761/nihms-1608087-f0006.jpg" itemprop="contentUrl" aria-describedby="figure-caption-0" role="button" data-image-width="812" data-image-height="2100" data-image-alt="Figure 1:" data-pmc-id="PMC7462118" data-figure-id="F1"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-0" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/5bbf0bce33b8/nihms-1608087-f0006.gif" alt="Figure 1:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="812"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="2100"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 1: </p> </strong> <div class="figure-caption-contents"><p> Translocation based fluorescent biosensors. A)… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 1: </p> </strong> <div class="figure-caption-contents"><p> Translocation based fluorescent biosensors. A) PH domains from different proteins are fused to… </p></div> </div> <figcaption id="figure-caption-0" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 1: </strong> <div class="figure-caption-contents">Translocation based fluorescent biosensors. A) PH domains from different proteins are fused to a FP and translocate to the plasma membrane upon the production of specific phosphoinositides<sup>,</sup>. For example, phosphorylation of PIP2 by PI3K to produce PIP3 at the plasma membrane causes translocation of the biosensor from the cytosol to the plasma membrane. B) Kinase translocation reporters utilize kinase specific substrate sequences within nuclear localization sequences (NLS) and/or nuclear export sequences (NES) to promote the import into the nucleus when dephosphorylated and export out of the nucleus when phosphorylated<sup>,</sup>.</div> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="1" data-label-slug="figure-2"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/2662a9704363/nihms-1608087-f0007.jpg" itemprop="contentUrl" aria-describedby="figure-caption-1" role="button" data-image-width="2045" data-image-height="1843" data-image-alt="Figure 2:" data-pmc-id="PMC7462118" data-figure-id="F2"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-1" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/6f9b50034481/nihms-1608087-f0007.gif" alt="Figure 2:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="2045"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1843"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 2: </p> </strong> <div class="figure-caption-contents"><p> Single FP fluorescent biosensor designs… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 2: </p> </strong> <div class="figure-caption-contents"><p> Single FP fluorescent biosensor designs for cellular analytes and membrane potential. Insertion of… </p></div> </div> <figcaption id="figure-caption-1" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 2: </strong> <div class="figure-caption-contents">Single FP fluorescent biosensor designs for cellular analytes and membrane potential. Insertion of a sensing unit into a FP. Calmodulin, mEpac and mPDE5α undergo conformational changes in response to binding Ca<sup>2+ </sup>, cAMP, and cGMP, respectively, which perturbs the chromophore and alters the fluorescence. Binding can either lead to an increase in fluorescence, as seen in the Ca<sup>2+</sup> biosensor camgaroo1, or a decrease in fluorescence, as seen in the cAMP biosensor flamindo. B) Sandwiching a cpFP between sensing units. Biosensors have utilized sensing units that comprise either separate receiver and switch domains<sup>,</sup> or split proteins that re-constitute during protein folding<sup>–</sup>. For example, GCaMP biosensors utilize separate domains of CaM and M13, where calcium binding to CaM promotes the binding of CaM to M13 and results in a conformational change that leads to an increase in GFP fluorescence. On the other hand, the membrane voltage sensor ASAP1 inserts cpGFP into the voltage-sensing domain of the chicken voltage-sensitive phosphatase Gg-VSP, which reconstitutes after folding and depolarization leads to a conformational change in the 4<sup>th</sup> transmembrane segment that alters the fluorescence of cpGFP C) Insertion of a FP into a voltage-sensitive channel. The conformational changes induced in voltage-sensitive K<sup>+</sup> and Na<sup>+</sup> channels alter the fluorescence of GFP to act as biosensors of membrane potential<sup>,</sup>.</div> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="2" data-label-slug="figure-3"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/c9d9e8575698/nihms-1608087-f0008.jpg" itemprop="contentUrl" aria-describedby="figure-caption-2" role="button" data-image-width="2053" data-image-height="1669" data-image-alt="Figure 3:" data-pmc-id="PMC7462118" data-figure-id="F3"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-2" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/5057d80578e0/nihms-1608087-f0008.gif" alt="Figure 3:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="2053"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1669"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 3: </p> </strong> <div class="figure-caption-contents"><p> Designs of FRET-based reporters for… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 3: </p> </strong> <div class="figure-caption-contents"><p> Designs of FRET-based reporters for ions, cellular analytes and membrane potential. FRET-based metal… </p></div> </div> <figcaption id="figure-caption-2" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 3: </strong> <div class="figure-caption-contents">Designs of FRET-based reporters for ions, cellular analytes and membrane potential. FRET-based metal ion biosensors utilize receiver and switch domains that bind to each other<sup>,</sup>, or endogenous proteins that undergo a conformational change in response to binding of metal ions. These conformational changes alter the intramolecular distance and orientation of a pair of FPs and thus changes the amount of energy transferred from the donor FP to the acceptor FP. B) Voltage sensors that utilize a FRET-based readout often rely primarily on changing the orientation of dipoles of FPs<sup>,</sup>. VSFP1 contains a CFP inserted between the 3<sup>rd</sup> and 4<sup>th</sup> transmembrane domain and a YFP fused to the C terminal tail of a truncated portion of the rat Kv2.1 channel where cell depolarization induces a conformational shift in the 4<sup>th</sup> transmembrane domain, thus changing the relative angle of the two dipoles of the fluorescent proteins. C) Several biosensors for cellular analytes have utilized the design of sandwiching a conformationally switching domain between a FP FRET pair<sup>,,,,,,,,</sup>. For example, the cAMP biosensor ICUE2 utilizes the conformational change induced by cAMP binding to the CNB domain of Epac1 to increase the intramolecular distance between the donor and acceptor FPs. Alternatively, having both a PIP3 binding PH domain from GRP1 and a C-terminal membrane localization sequence in combination with engineered rigid α-helical linkers yielded a chimeric protein that exhibits significant conformational changes in response to PIP3 production, leading to changes in FRET between a FRET pair flanking the chimeric protein.</div> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="3" data-label-slug="figure-4"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/f6b79807a3ec/nihms-1608087-f0009.jpg" itemprop="contentUrl" aria-describedby="figure-caption-3" role="button" data-image-width="1801" data-image-height="2040" data-image-alt="Figure 4:" data-pmc-id="PMC7462118" data-figure-id="F4"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-3" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/4dea46ea188b/nihms-1608087-f0009.gif" alt="Figure 4:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="1801"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="2040"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 4: </p> </strong> <div class="figure-caption-contents"><p> FRET-based biosensor designs for signaling… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 4: </p> </strong> <div class="figure-caption-contents"><p> FRET-based biosensor designs for signaling proteins. Biosensors for signaling enzyme activation consist of… </p></div> </div> <figcaption id="figure-caption-3" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 4: </strong> <div class="figure-caption-contents">FRET-based biosensor designs for signaling proteins. Biosensors for signaling enzyme activation consist of whole or truncated portions of the enzyme sandwiched between a FP FRET pair<sup>,,,</sup>. For example, the conformational change in the phosphatase CaNAα upon activation by Ca<sup>2+</sup> bound CaM increases the distance between the two FPs in CaNARi. B) The insertion of FPs into a receptor or between a GPCR and Gαs have been utilized to create FRET-based biosensors of receptor activation. C) Biosensors for small G-protein activation utilize G-protein binding domains (e.g., Raf RBD, PKN RBD, EEA1 RBD), which bind to specific G-proteins upon activation to create a conformational change<sup>,,,,</sup>. D) FRET-based biosensors for kinase activity consist of either an endogenous kinase substrate or a kinase substrate sequence paired with a phospho-amino acid binding domain<sup>,,,,</sup> sandwiched between two FPs, which undergo a conformational change upon phosphorylation. Conversely, the phosphatase biosensor CaNAR uses a fragment of NFAT1c, which is phosphorylated at basal levels and exhibits a conformational shift upon dephosphorylation by CaN. E) Similarly, biosensors for other PTMs use substrates paired with protein domains that recognize the modified substrates. The Histac biosensors contain a full-length histone, H3 or H4, and a fragment of a bromodomain (BRD)containing protein that binds the acetylated substrate<sup>,</sup>.</div> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="4" data-label-slug="figure-5"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/8138bda0d4da/nihms-1608087-f0010.jpg" itemprop="contentUrl" aria-describedby="figure-caption-4" role="button" data-image-width="945" data-image-height="2100" data-image-alt="Figure 5:" data-pmc-id="PMC7462118" data-figure-id="F5"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-4" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/03186cb40bed/nihms-1608087-f0010.gif" alt="Figure 5:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="945"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="2100"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 5: </p> </strong> <div class="figure-caption-contents"><p> ddFP- and luminescence-based biosensors. Bimolecular… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 5: </p> </strong> <div class="figure-caption-contents"><p> ddFP- and luminescence-based biosensors. Bimolecular (top) or unimolecular (bottom) Ca <sup> 2+ </sup> -biosensor designs… </p></div> </div> <figcaption id="figure-caption-4" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 5: </strong> <div class="figure-caption-contents">ddFP- and luminescence-based biosensors. Bimolecular (top) or unimolecular (bottom) Ca<sup>2+</sup>-biosensor designs based on ddFPs<sup>,</sup>. The dimeriaztion of the dim ddFP partner, FP-B, with either RFP-A or GFP-A increases their fluorescence, and this dimerization is modulated by the Ca<sup>2+</sup>dependent binding of calmodulin to M13. B) Modulating the structure of split luciferaces by conformational switches has been utilized in cAMP, PKA and Ca<sup>2+</sup> biosensors<sup>,,</sup>. In the FLuc cAMP sensor, the conformational switch induced by binding of cAMP to PKA RIIβ allows the firefly luciferase (FLuc) to properly form, thus allowing the enzyme to catalyze the degradation of luciferin and emit photons. Similarly, the Nano-lantern Ca<sup>2+</sup> biosensor undergoes a Ca<sup>2+</sup>-dependent reconstitution of renilla luciferace (RLuc), which is then capable of BRET with the adjacent, brighter YFP.</div> </figcaption> </figure> <figure class="figure-item " itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="5" data-label-slug="figure-6"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/48eab9a245d0/nihms-1608087-f0011.jpg" itemprop="contentUrl" aria-describedby="figure-caption-5" role="button" data-image-width="1941" data-image-height="1970" data-image-alt="Figure 6:" data-pmc-id="PMC7462118" data-figure-id="F6"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-5" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/702c6098a1c3/nihms-1608087-f0011.gif" alt="Figure 6:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="1941"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1970"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 6: </p> </strong> <div class="figure-caption-contents"><p> Coupled reporter systems. (A and… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 6: </p> </strong> <div class="figure-caption-contents"><p> Coupled reporter systems. (A and B) Biosensors reporting the cell cycle phase of… </p></div> </div> <figcaption id="figure-caption-5" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 6: </strong> <div class="figure-caption-contents">Coupled reporter systems. (A and B) Biosensors reporting the cell cycle phase of cells utilize fluorescent proteins fused to protein fragments that are selectively degraded during specific phases of the cell cycle<sup>,</sup>. In the Fucci system, Gem is degraded during the G1 and late M phases and, conversely, Cdt1is degraded in S and G2 phases. The improved Fucci4 adds the condensation of chromatin around Histone H1 to report the M phase, as well as SLBP, which is degraded after S phase, in addition to labeled Cdt1 and Gem from Fucci. C) The chimeric receptor BBD-ECat, which consists of an extracellular TrkB domain, which dimerizes upon binding BDNF, and an intracellular EGFR domain, is coupled with an EGFR activity reporter ECaus to act as a reporter for BDNF release by neurons. D) Similarly, the expression of the M1 receptor and the Ca<sup>2+</sup> biosensor TN-XXL in a co-cultured reporter cell, uses the endogenous coupling of the GPCR M1R to Gα<sub>q</sub> which, upon receptor stimulation, leads to an increase in intracellular Ca<sup>2+</sup> to report the presense of a neurotransmitter, acetylcholine (Ach).</div> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="6" data-label-slug="figure-7"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/0098cd27a6fd/nihms-1608087-f0012.jpg" itemprop="contentUrl" aria-describedby="figure-caption-6" role="button" data-image-width="2040" data-image-height="1459" data-image-alt="Figure 7:" data-pmc-id="PMC7462118" data-figure-id="F7"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-6" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/f45e780b0a2e/nihms-1608087-f0012.gif" alt="Figure 7:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="2040"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1459"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 7: </p> </strong> <div class="figure-caption-contents"><p> Infrared FP-based Caspase-3 reporter, iCasper.… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 7: </p> </strong> <div class="figure-caption-contents"><p> Infrared FP-based Caspase-3 reporter, iCasper. The introduction of the Caspase-3 cleavage sequence into… </p></div> </div> <figcaption id="figure-caption-6" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 7: </strong> <div class="figure-caption-contents">Infrared FP-based Caspase-3 reporter, iCasper. The introduction of the Caspase-3 cleavage sequence into the circularly permuted mIFP prevents the incorporation of BV into the GAF domain, but cleavage by Caspase-3 liberates the catalytic cysteine to promote the incorporation of BV and the formation of the chromophore.</div> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="7" data-label-slug="figure-8"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/ce1409a13b6f/nihms-1608087-f0013.jpg" itemprop="contentUrl" aria-describedby="figure-caption-7" role="button" data-image-width="1567" data-image-height="2040" data-image-alt="Figure 8:" data-pmc-id="PMC7462118" data-figure-id="F8"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-7" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/2a46f93c3447/nihms-1608087-f0013.gif" alt="Figure 8:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="1567"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="2040"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 8: </p> </strong> <div class="figure-caption-contents"><p> Photoconversion-based snapshot recorder of Ca… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 8: </p> </strong> <div class="figure-caption-contents"><p> Photoconversion-based snapshot recorder of Ca <sup> 2+ </sup> . CaMPARi acts similarly to other green… </p></div> </div> <figcaption id="figure-caption-7" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 8: </strong> <div class="figure-caption-contents">Photoconversion-based snapshot recorder of Ca<sup>2+</sup>. CaMPARi acts similarly to other green single color Ca<sup>2+</sup> biosensors where the green fluorescence intensity is dependent on the Ca<sup>2+</sup> concentration (left), except it can also “record” the presence of high Ca<sup>2+</sup> during a snapshot in time. When the CaMPARi biosensor is illuminated with blue/violet light and the intracellular Ca<sup>2+</sup> is high, the biosensor will irreversibly convert to a red (right) Ca<sup>2+</sup> biosensor.</div> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="8" data-label-slug="figure-9"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/fb6ea6878d13/nihms-1608087-f0014.jpg" itemprop="contentUrl" aria-describedby="figure-caption-8" role="button" data-image-width="1931" data-image-height="1715" data-image-alt="Figure 9:" data-pmc-id="PMC7462118" data-figure-id="F9"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-8" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/623548aa2ab9/nihms-1608087-f0014.gif" alt="Figure 9:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="1931"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1715"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 9: </p> </strong> <div class="figure-caption-contents"><p> Transcription-based snapshot reporters. The Cal-Light… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 9: </p> </strong> <div class="figure-caption-contents"><p> Transcription-based snapshot reporters. The Cal-Light and FLARE Ca <sup> 2+ </sup> biosensors create an “AND… </p></div> </div> <figcaption id="figure-caption-8" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 9: </strong> <div class="figure-caption-contents">Transcription-based snapshot reporters. The Cal-Light and FLARE Ca<sup>2+</sup> biosensors create an “AND gate” design by combining the optogenetically controlled AsLOV2 domain with a Ca<sup>2+</sup> switch controlled split-TEV protease, which leads to the cleavage of the tTa-VP16 transcriptional activator only in the presence of high Ca<sup>2+</sup> and blue light<sup>,</sup>. The cleavage of the transcriptional activator will then turn on the expression of a reporter gene such as GFP expression.</div> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="9" data-label-slug="figure-10"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/b2ae46f52eb8/nihms-1608087-f0015.jpg" itemprop="contentUrl" aria-describedby="figure-caption-9" role="button" data-image-width="2100" data-image-height="1172" data-image-alt="Figure 10:" data-pmc-id="PMC7462118" data-figure-id="F10"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-9" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/42a564bf0900/nihms-1608087-f0015.gif" alt="Figure 10:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="2100"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1172"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 10: </p> </strong> <div class="figure-caption-contents"><p> Fluctuation-based PKA biosensor FLINC-AKAR1. Fluorescence… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 10: </p> </strong> <div class="figure-caption-contents"><p> Fluctuation-based PKA biosensor FLINC-AKAR1. Fluorescence of TagRFP-T is modulated by its interaction with… </p></div> </div> <figcaption id="figure-caption-9" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 10: </strong> <div class="figure-caption-contents">Fluctuation-based PKA biosensor FLINC-AKAR1. Fluorescence of TagRFP-T is modulated by its interaction with Dronpa through a process termed FLINC, such that when in close proximity to Dronpa, TagRFP-T exhibits increased fluorescence fluctuation. This enables this biosensor to report PKA activity at a sub-diffraction spatial resolution through the use of the super-resolution technique pcSOFI.</div> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="10" data-label-slug="figure-11"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/2979a33b238e/nihms-1608087-f0016.jpg" itemprop="contentUrl" aria-describedby="figure-caption-10" role="button" data-image-width="2087" data-image-height="1985" data-image-alt="Figure 11:" data-pmc-id="PMC7462118" data-figure-id="F11"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-10" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/0c626a7fb11e/nihms-1608087-f0016.gif" alt="Figure 11:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="2087"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1985"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 11: </p> </strong> <div class="figure-caption-contents"><p> Temporal dynamics quantified by fluorescent… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 11: </p> </strong> <div class="figure-caption-contents"><p> Temporal dynamics quantified by fluorescent biosensors. The kinetics of GPCR signaling. First, the… </p></div> </div> <figcaption id="figure-caption-10" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 11: </strong> <div class="figure-caption-contents">Temporal dynamics quantified by fluorescent biosensors. The kinetics of GPCR signaling. First, the receptor undergoes a conformational change in response to ligand binding, which was observed to occur with a time constant on the order of 40 ms in the α<sub>2</sub>-Adrenergic Receptor using the biosensor α<sub>2</sub>-AR-CAM. Next, the heterotrimeric G protein associates with the activated receptor. A2A Adenosine Receptor, A<sub>2A</sub>R, fused with YFP at the C-terminal tail and CFP-labeled Gγ2 exhibited an increase in FRET upon stimulation with adenosine with a time constant of approximately 50 ms. Finally, the receptor stimulates the exhange of GTP for GDP on the Gα subunit, thus activating Gα and promoting the dissociation of Gα from Gβγ. Biosensors consisting of a CFP-labeled Gγ subunit and either Gαi or Gαs fused to YFP showed activation time constants on the order of 500 ms. B) Adaptation is the return of a signaling pathway towards its previous state while under continued stimulation. PC12 cells expressing the ERK biosensor EKAR show an adaptive response to EGF stimulation (gray lines) but a much more sustained response to NGF stimulation (black lines), reproduced with permission from Herbst et al. Mol and Cell Bio 2011. Negative feedback by ERK onto Raf activation is hypothesized to lead to a transient response, whereas positive feedback in the NGF signaling pathway is hypothesized to lead to bistability. C) Oscillations are the regular or semi-regular cycling between activity/concentration states, as can be seen in the TEA-induced Ca<sup>2+</sup> and PKA activity oscillations in MIN6 cells. These oscillations were observed through the simultaneous measurement of Ca<sup>2+</sup> concentration with the Ca<sup>2+</sup> dye Fura-2 (black line) and a Green/Red variant of AKAR, GR-AKAR, (red line) at the single-cell level in MIN6 cells. While the inhibition of K<sup>+</sup> channels by ATP and the interplay between voltage and Ca<sup>2+</sup> are the primary driver of the Ca<sup>2+</sup> oscillations, the negative feedback of Ca<sup>2+</sup> through cAMP and PKA is hypothesized to strengthen these oscillations and tune their frequencies. Reproduced with permission from Ni et al. Nat Chem Bio 2011. D) Bistability and ultrasensitivity describe phenomena wherein a signaling pathway is insensitive to stimulation below a certain threshold dose but responds in a switch-like fashion to super-threshold stimuli. HeLa cells expressing the JNK biosensor JNKAR1 did not respond to anisomycin at concentrations of 20 (cyan diamonds), or 50 (blue triangles) nM, but exhibited a strong response to 500 nM (red circle) and 5 μM (black squares), reproduced with permission from Fosbrink et al. PNAS 2010. In addition to the multistep activation along the JNK signaling pathway, positive feedback by JNK onto the activation of upstream regulator MKK7 is hypothesized to contribute to the ultrasensitive nature of activation by anisomycin.</div> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="11" data-label-slug="figure-12"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/4148c122fd94/nihms-1608087-f0017.jpg" itemprop="contentUrl" aria-describedby="figure-caption-11" role="button" data-image-width="1590" data-image-height="2040" data-image-alt="Figure 12:" data-pmc-id="PMC7462118" data-figure-id="F12"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-11" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/7f3470335be3/nihms-1608087-f0017.gif" alt="Figure 12:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="1590"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="2040"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 12: </p> </strong> <div class="figure-caption-contents"><p> Probing spatial compartmentalization with fluorescent… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 12: </p> </strong> <div class="figure-caption-contents"><p> Probing spatial compartmentalization with fluorescent biosensors. GPCRs are activated at the plasma membrane… </p></div> </div> <figcaption id="figure-caption-11" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 12: </strong> <div class="figure-caption-contents">Probing spatial compartmentalization with fluorescent biosensors. GPCRs are activated at the plasma membrane but can continue to stimulate downstream signaling after internalization. Activation of β<sub>2</sub>AR at the plasma membrane by an extracellular ligand leads to the production of cAMP. This activation of β<sub>2</sub>AR can be monitored both directly, by GFP labeled nanobody Nb80 which binds the active conformation of β<sub>2</sub>AR, and indirectly, through biosensors of cAMP concentration such as Epac1-camps. The activation of GPCRs is reversed by the binding of βarrestin (βarr) which attenuates downstream signaling and promotes receptor trafficking to clathrin-coated pits for endocytosis. After internalization, βarr can dissociate from the receptor and some GPCRs have been shown to then continue downstream signaling from the endosome, as shown by Nb80-GFP translocation to the endosomes and monitoring of cAMP production after perturbing internalization or blocking the membrane pool of the receptors by using membrane-impermeable antagonists. B) The fusion of targeting domains to biosensors enables the measurement the signaling dynamics in several different subcellular microdomains by promoting the trafficking and localization of the biosensor to these regions. C) For example, Zhou et al. targeted TORCAR to the plasma membrane (PM-TORCAR), lysosome (Lyso-TORCAR) and nucleus (TORCAR-NLS) to examine the mTORC1 activity in each of these microdomains. With these targeted biosensors, it was shown that growth factor stimulation leads to mTORC1 activation in all three microdomains, whereas stimulation with nutrients activated mTORC1 at the lysosome and nucleus but not at the plasma membrane.</div> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="12" data-label-slug="figure-13"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/7e4f5e662174/nihms-1608087-f0018.jpg" itemprop="contentUrl" aria-describedby="figure-caption-12" role="button" data-image-width="1942" data-image-height="1577" data-image-alt="Figure 13:" data-pmc-id="PMC7462118" data-figure-id="F13"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-12" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/f144facc81fd/nihms-1608087-f0018.gif" alt="Figure 13:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="1942"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1577"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 13: </p> </strong> <div class="figure-caption-contents"><p> Spatial regulation of diffusible signaling… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 13: </p> </strong> <div class="figure-caption-contents"><p> Spatial regulation of diffusible signaling molecules. Spatial Ca <sup> 2+ </sup> gradients are primarily shaped… </p></div> </div> <figcaption id="figure-caption-12" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 13: </strong> <div class="figure-caption-contents">Spatial regulation of diffusible signaling molecules. Spatial Ca<sup>2+</sup> gradients are primarily shaped by 4 effects: the rate of Ca<sup>2+</sup> influx into the cytosol from the extracellular environment and intracellular stores, the efflux of Ca<sup>2+</sup> out of the cytosol by Ca<sup>2+</sup> pumps, Ca<sup>2+</sup> binding proteins which buffer the changes in the free Ca<sup>2+</sup> concentration and diffusion of Ca<sup>2+</sup> through the cytosol. B) While the effect of Ca<sup>2+</sup> influx by a single channel may be limited by the rate of Ca<sup>2+</sup> flux through the channel and the rate of diffusion (top), Ca<sup>2+</sup>-induced Ca<sup>2+</sup> release (CICR), where an increase in Ca<sup>2+</sup> can stimulate the opening of neighboring Ca<sup>2+</sup> channels, creates a positive feedback loop that can create a wave of Ca<sup>2+</sup> release that spreads faster than possible by diffusion alone (bottom). C) The spatial arrangement of adenylyl cyclases (ACs), which produce cAMP, and phosphodiesterases (PDEs), which degrade cAMP, regulate the formation of gradients of the diffusible messenger cAMP. For example, rat hippocampal neurons at 5 days in vitro (DIV5), when the axons have started to become more thoroughly established, have been observed to exhibit an axon-directed gradient of cAMP accumulation in response to Forskolin stimulation of ACs. Conversely, at an earlier time point of 3 days in vitro (DIV3), cAMP production was observed to be much more limited in response to Forskolin, which was hypothesized to be due to negative feedback mediated by PKA and PDE, which are localized to distal regions of an axon by an A-Kinase Anchoring Protein (AKAP).</div> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="13" data-label-slug="figure-14"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/f1e591c7eaba/nihms-1608087-f0019.jpg" itemprop="contentUrl" aria-describedby="figure-caption-13" role="button" data-image-width="1163" data-image-height="2040" data-image-alt="Figure 14:" data-pmc-id="PMC7462118" data-figure-id="F14"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-13" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/766d1ad338ec/nihms-1608087-f0019.gif" alt="Figure 14:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="1163"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="2040"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 14: </p> </strong> <div class="figure-caption-contents"><p> Integrated approach combining biosensor imaging… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 14: </p> </strong> <div class="figure-caption-contents"><p> Integrated approach combining biosensor imaging and computational modeling. A) Computational model development utilizes… </p></div> </div> <figcaption id="figure-caption-13" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 14: </strong> <div class="figure-caption-contents">Integrated approach combining biosensor imaging and computational modeling. A) Computational model development utilizes an iterative approach where the hypothesized structure of the signaling network is implemented into a computational model (top). The results of the computational model are then compared with experimental biosensor data, which serves to approximate unknown model parameters (model fitting) and identify aspects of the experimental data that the model is not capturing. This comparison is then used to refine the hypothesized model structure. This process is iterated until the model adequately reflects the experimental data. This model is then, in turn, capable of generating previously untested conditions (e.g., different stimulation, inhibition of a signaling enzyme) to generate new hypotheses (bottom). Biosensors then serve as a powerful tool to validate these model predictions. B&C) Kinetic computational models simulate changes in the activity and concentration of different signaling reactants as defined by the hypothsized connections within the signaling networks. These models do not directly approximate changes in space (B) but sub-cellular compartments can be defined where specific model species can exchange between compartments (C). D) Spatiotemporal models simulate the changes in signal transduction across both space and time; therefore, the model outputs the model species concentration or activity as it varies across the defined geometry and through time.</div> </figcaption> </figure> <figure class="figure-item tail" itemscope itemtype="http://schema.org/ImageObject" itemprop="associatedMedia" data-slide-index="14" data-label-slug="figure-15"> <a class="figure-link" href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/29d55605f6e4/nihms-1608087-f0020.jpg" itemprop="contentUrl" aria-describedby="figure-caption-14" role="button" data-image-width="1395" data-image-height="2040" data-image-alt="Figure 15:" data-pmc-id="PMC7462118" data-figure-id="F15"> <img class="figure-thumb" itemprop="thumbnail" id="article-image-14" src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4389/7462118/983361059adb/nihms-1608087-f0020.gif" alt="Figure 15:" /> </a> <meta itemprop="width" itemtype="http://schema.org/ImageObject" content="1395"> <meta itemprop="height" itemtype="http://schema.org/ImageObject" content="2040"> <div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true"> <div class="caption-wrap"> <strong class="figure-label"> <p> Figure 15: </p> </strong> <div class="figure-caption-contents"><p> Single-color FRET-based methods. Fluorescence quenching… </p></div> </div> </div> <div class="figure-caption-medium figure-caption-text" aria-hidden="true"> <strong class="figure-label"> <p> Figure 15: </p> </strong> <div class="figure-caption-contents"><p> Single-color FRET-based methods. Fluorescence quenching resonance energy transfer (FqRET)-based CaMKIIα biosensor green-Camuiα. In… </p></div> </div> <figcaption id="figure-caption-14" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description"> <strong class="figure-label"> Figure 15: </strong> <div class="figure-caption-contents">Single-color FRET-based methods. Fluorescence quenching resonance energy transfer (FqRET)-based CaMKIIα biosensor green-Camuiα. In the inactive state, the energy from excited EGFP is non-radiatively transferred to the dark acceptor REACh, which then dissipates that energy without emitting a photon. Upon activation, the conformational change moves REACh away from the EGFP, leading to increased EGFP emission. FqRET can also be quantified by fluorescence lifetime imaging (FLIM), where the lifetime, τ, is low in the high-FqRET state and vice versa. B) Homo-FRET-based NADP+ biosensor Apollo-NADP+. Upon binding NADP+, G6PD dimerizes and thus allow FRET between the two FPs. Fluorescence polarization microscopy can be used where a polarized excitation source will only excite FP in the appropriate dipole orientation, resulting in the emitted photon also being polarized. Conversely, when FRET occurs between these two FPs, the emitted photons exhibit a mix of polarizations and thus decrease the polarized fluorescence signal.</div> </figcaption> </figure> </div> <div class="see-all actions-bar"> <button class="show-more" aria-controls="slides-container" data-ga-category="figure" data-ga-action="show_all"> All figures (15) </button> </div>  <div class="pswp figures-dialog" id="figures-dialog" tabindex="-1" role="dialog" aria-hidden="true" data-image-gallery-uid="article-figures" aria-label="Figures Dialog">  <div class="pswp__bg"></div>  <div class="pswp__scroll-wrap">  <div class="pswp__container"> <div class="pswp__item"></div> <div class="pswp__item"></div> <div class="pswp__item"></div> </div>  <div class="pswp__ui pswp__ui--hidden"> <div class="pswp__top-bar">  <div class="pswp__counter"></div> <button class="pswp__button pswp__button--close" title="Close (Esc)"></button> <button class="pswp__button pswp__button--share" title="Share"></button> <button class="pswp__button pswp__button--fs" title="Toggle fullscreen"></button> <button class="pswp__button pswp__button--zoom" title="Zoom in/out"></button>   <div class="pswp__preloader"> <div class="pswp__preloader__icn"> <div class="pswp__preloader__cut"> <div class="pswp__preloader__donut"></div> </div> </div> </div> </div> <div class="pswp__share-modal pswp__share-modal--hidden pswp__single-tap"> <div class="pswp__share-tooltip"></div> </div> <button class="pswp__button pswp__button--arrow--left" title="Previous (arrow left)"> </button> <button class="pswp__button pswp__button--arrow--right" title="Next (arrow right)"> </button> <div class="pswp__caption"> <div class="pswp__caption__center"></div> </div> </div> </div> </div> <div class="figure-dialog-caption"> <div class="figure-caption-link-container"> <a class="figure-caption-link" href="#">See this image and copyright information in PMC</a> </div> <div class="figure-caption-center" data-expand-title="click to show full caption" data-collapse-title="click to hide full caption"> </div> <button class="figure-caption-toggle-btn" aria-label="show full caption" data-expand-text="show full caption" data-collapse-text="hide full caption">  </button> </div> </div> <div class="similar-articles" id="similar"> <h2 class="title"> Similar articles </h2> <ul class="articles-list" id="similar-articles-list"> <li class="full-docsum"> <div class="docsum-content" style="display:inline;"> <a class="docsum-title" href="/32058267/" ref="article_id=32058267&linksrc=similar_articles_link&ordinalpos=1" data-ga-category="similar_article" data-ga-action="32058267" data-ga-label=""> Recent advances in the use of genetically encodable optical tools to elicit and monitor signaling events. </a> <div class="docsum-citation full-citation"> <span class="docsum-authors full-authors">Lee HN, Mehta S, Zhang J.</span> <span class="docsum-authors short-authors">Lee HN, et al.</span> <span class="docsum-journal-citation full-journal-citation">Curr Opin Cell Biol. 2020 Apr;63:114-124. doi: 10.1016/j.ceb.2020.01.007. 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Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks - PubMed