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in vivo replacement GMP Quality Control Testing services

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class="et_pb_menu_page_id-1164 menu-item menu-item-type-post_type menu-item-object-page menu-item-1245"><a href="https://pathoquest.com/why-us/idtect-platform/">iDTECT<sup>®</sup> Platform</a></li> <li class="et_pb_menu_page_id-293 menu-item menu-item-type-post_type menu-item-object-page menu-item-371"><a href="https://pathoquest.com/why-us/3rs-2/">3Rs</a></li> <li class="et_pb_menu_page_id-408 menu-item menu-item-type-post_type menu-item-object-page menu-item-has-children menu-item-412"><a href="https://pathoquest.com/quality-regulatory/">Quality &#038; Regulatory</a> <ul class="sub-menu"> <li class="et_pb_menu_page_id-1622 menu-item menu-item-type-post_type menu-item-object-page menu-item-1692"><a href="https://pathoquest.com/quality/">Quality</a></li> <li class="et_pb_menu_page_id-1632 menu-item menu-item-type-post_type menu-item-object-page menu-item-1691"><a href="https://pathoquest.com/regulatory/">Regulatory</a></li> </ul> </li> <li class="et_pb_menu_page_id-951 menu-item menu-item-type-post_type menu-item-object-page menu-item-1019"><a href="https://pathoquest.com/resources/">Resources</a></li> <li class="et_pb_menu_page_id-449 menu-item menu-item-type-post_type menu-item-object-page menu-item-460"><a href="https://pathoquest.com/news-and-events/">News</a></li> <li class="de-menu-cta et_pb_menu_page_id-1510 menu-item menu-item-type-post_type menu-item-object-page menu-item-1553"><a href="https://pathoquest.com/contact-us/">Contact</a></li> </ul></nav> </div> <div class="et_mobile_nav_menu"> <div class="mobile_nav closed"> <span class="mobile_menu_bar"></span> </div> </div> </div> </div> </div> </div> </div> </div> </div> </header> <div id="et-main-area"> <div id="main-content"> <article id="post-286" class="post-286 page type-page status-publish has-post-thumbnail hentry"> <div class="entry-content"> <div class="et-l et-l--post"> <div class="et_builder_inner_content et_pb_gutters3"> <div class="et_pb_section et_pb_section_0 et_pb_section_parallax et_pb_with_background et_section_regular" > <span class="et_parallax_bg_wrap"><span class="et_parallax_bg" style="background-image: url(https://pathoquest.com/wp-content/uploads/2023/07/in_vivo_banner.webp);" ></span></span> <div class="et_pb_row et_pb_row_0 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_0 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_image et_pb_image_0"> <span class="et_pb_image_wrap "><img decoding="async" src="https://pathoquest.com/wp-content/uploads/2023/07/Icons_3Rs.svg" alt="" title="Icons_3Rs" class="wp-image-341" /></span> </div><div class="et_pb_module et_pb_text et_pb_text_0 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h1 style="text-align: center;"><em>In Vivo</em> Replacement</h1> <p style="text-align: center;">NGS as an ethical alternative to animals in biosafety testing and characterization.</p></div> </div> </div> </div><div class="et_pb_row et_pb_row_1 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_column et_pb_column_1_2 et_pb_column_1 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_image et_pb_image_1"> <span class="et_pb_image_wrap "><img fetchpriority="high" decoding="async" width="3369" height="2382" src="https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-VIROME-HR.png" alt="" title="iDTECT VIROME- HR" srcset="https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-VIROME-HR.png 3369w, https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-VIROME-HR-1280x905.png 1280w, https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-VIROME-HR-980x693.png 980w, https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-VIROME-HR-480x339.png 480w" sizes="(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 3369px, 100vw" class="wp-image-1227" /></span> </div> </div><div class="et_pb_column et_pb_column_1_2 et_pb_column_2 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_image et_pb_image_2"> <span class="et_pb_image_wrap "><img loading="lazy" decoding="async" width="3368" height="2382" src="https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-TRANSCRIPTOME-HR.png" alt="" title="iDTECT TRANSCRIPTOME- HR" srcset="https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-TRANSCRIPTOME-HR.png 3368w, https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-TRANSCRIPTOME-HR-1280x905.png 1280w, https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-TRANSCRIPTOME-HR-980x693.png 980w, https://pathoquest.com/wp-content/uploads/2024/03/iDTECT-TRANSCRIPTOME-HR-480x339.png 480w" sizes="(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 3368px, 100vw" class="wp-image-1226" /></span> </div> </div> </div> </div><div class="et_pb_section et_pb_section_1 et_pb_with_background et_section_regular" > <div class="et_pb_row et_pb_row_2 et_pb_equal_columns et_pb_gutters3"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_3 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_1 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2 style="text-align: center;">What is <em>in vivo</em> replacement and why is it important?</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_0 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div><div class="et_pb_module et_pb_text et_pb_text_2 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p>The use of animal models in the characterization and biosafety testing of biologic medicines has been a staple for decades. Despite their limitations, <em>in vivo</em> testing has remained essential in biologic cell line and viral vector characterization due to the need for prior knowledge of contaminants when using PCR or ELISA. Agnostic cell culture-based methods also fall short as they detect a narrow range of adventitious pathogens. Consequently, animal models, despite their limited sensitivity in detecting a broad range of viruses, have continued as a crucial complementary method.</p> <p>However, the landscape is changing with the advent of alternatives to animal testing, such as <em>in vitro</em> testing . These <em>in vitro </em>assay methods align with the 3Rs of animal research (Replacement, Reduction, and Refinement), aiming to minimize animal use in scientific research. <em>In vitro</em> testing methods are increasingly recognized for their potential to provide reliable and sensitive detection of contaminants without the ethical and practical issues of animal models.</p> <p>Next-generation sequencing (NGS) has been used for agnostic detection of unknown pathogens in clinical settings and commercial biopharmaceutical manufacturing, especially for contamination investigations. Moreover the ICHQ5A (R2) and European Pharmacopoeia encourages the replacement of i<em>n vivo</em> testing with NGS. “</p> <p>In collaboration with Charles River Laboratories, PathoQuest has become the first GMP Contract Research Organization (CRO) to demonstrate conclusively that iDTECT® NGS assays are superior to conventional <em>in vivo </em>biosafety testing and characterization methods. This breakthrough, along with regulatory bodies&#8217; commitment to eliminate animal models in most submissions, signals that now is the ideal time to transition from <em>in vivo</em> testing and embrace <em>NGS</em> testing methods as the new standard for biosafety and characterization in biologics.</p></div> </div><div class="et_pb_button_module_wrapper et_pb_button_0_wrapper et_pb_button_alignment_left et_pb_module "> <a class="et_pb_button et_pb_button_0 et_pb_bg_layout_light" href="https://pathoquest.com/why-us/3rs-2/" target="_blank">FIND OUT MORE ABOUT 3Rs</a> </div> </div> </div><div class="et_pb_row et_pb_row_3 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_column et_pb_column_1_6 et_pb_column_4 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_button_module_wrapper et_pb_button_1_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_1 et_pb_bg_layout_light" href="#WWD">WHAT WE DO</a> </div> </div><div class="et_pb_column et_pb_column_1_6 et_pb_column_5 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_button_module_wrapper et_pb_button_2_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_2 et_pb_bg_layout_light" href="#WTA">WHY THIS APPROACH</a> </div> </div><div class="et_pb_column et_pb_column_1_6 et_pb_column_6 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_button_module_wrapper et_pb_button_3_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_3 et_pb_bg_layout_light" href="#SR">Our comparison data</a> </div> </div><div class="et_pb_column et_pb_column_1_6 et_pb_column_7 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_button_module_wrapper et_pb_button_4_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_4 et_pb_bg_layout_light" href="#CS">CHALLENGES SOLVED</a> </div> </div><div class="et_pb_column et_pb_column_1_6 et_pb_column_8 et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_button_module_wrapper et_pb_button_5_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_5 et_pb_bg_layout_light" href="#OS">OTHER SERVICES</a> </div> </div><div class="et_pb_column et_pb_column_1_6 et_pb_column_9 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_button_module_wrapper et_pb_button_6_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_6 et_pb_bg_layout_light" href="#contact">CONTACT US</a> </div> </div> </div> </div><div id="WWD" class="et_pb_section et_pb_section_2 et_pb_with_background et_pb_section_parallax et_section_regular" > <div class="et_pb_row et_pb_row_4 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_column et_pb_column_2_5 et_pb_column_10 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_3 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2>What we do</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_1 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div><div class="et_pb_module et_pb_text et_pb_text_4 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p><b>Modalities Tested:</b></p> <ul> <li><span style="color: #2e3180;">mAbs and recombinant proteins biologics</span></li> <li><span style="color: #2e3180;">Viral vectors</span></li> <li><span style="color: #2e3180;">Cell therapies</span></li> <li><span style="color: #2e3180;">Vaccines</span></li> <li><span style="color: #2e3180;">RNA based therapeutics</span></li> <li><span style="color: #2e3180;">Cultured meat</span></li> </ul> <p><b>Benefits of iDTECT<sup>®</sup> NGS assays</b></p> <p><span style="color: #f6a800;"><code class="hljs language-css"><span class="hljs-string">✓ </span></code></span>GMP validated – <i>in vivo </i>testing mostly GLP<br /><span style="color: #f6a800;"><code class="hljs language-css"><span class="hljs-string">✓ </span></code></span>Fast results – days vs weeks<br /><span style="color: #f6a800;"><code class="hljs language-css"><span class="hljs-string">✓ </span></code></span>Much more reliable and robust than animal models<br /><span style="color: #f6a800;"><code class="hljs language-css"><span class="hljs-string">✓ </span></code></span>No need to generate neutralizing antibodies<br /><span style="color: #f6a800;"><code class="hljs language-css"><span class="hljs-string">✓ </span></code></span>Specific identification of contamination<br /><span style="color: #f6a800;"><code class="hljs language-css"><span class="hljs-string">✓ </span></code></span>Meet corporate 3Rs objectives</p> <p><strong>Our Assays:</strong></p> <ul> <li><span style="color: #2e3180;">iDTECT<sup>®</sup> Virome</span></li> <li><span style="color: #2e3180;">iDTECT<sup>®</sup> Transcriptome</span></li> </ul></div> </div> </div><div class="et_pb_column et_pb_column_3_5 et_pb_column_11 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_5 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2 style="text-align: center;">Advantages of NGS over Animal Testing</h2></div> </div><div class="et_pb_module et_pb_image et_pb_image_3"> <a href="https://pathoquest.com/wp-content/uploads/2023/07/In-Vivo-replacement_graphic@3x.png" class="et_pb_lightbox_image" title=""><span class="et_pb_image_wrap "><img loading="lazy" decoding="async" width="2934" height="3430" src="https://pathoquest.com/wp-content/uploads/2023/07/In-Vivo-replacement_graphic@3x.png" alt="" title="In Vivo replacement_graphic@3x" srcset="https://pathoquest.com/wp-content/uploads/2023/07/In-Vivo-replacement_graphic@3x.png 2934w, https://pathoquest.com/wp-content/uploads/2023/07/In-Vivo-replacement_graphic@3x-1280x1496.png 1280w, https://pathoquest.com/wp-content/uploads/2023/07/In-Vivo-replacement_graphic@3x-980x1146.png 980w, https://pathoquest.com/wp-content/uploads/2023/07/In-Vivo-replacement_graphic@3x-480x561.png 480w" sizes="(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 2934px, 100vw" class="wp-image-596" /></span></a> </div> </div> </div> </div><div id="WTA" class="et_pb_section et_pb_section_3 et_pb_with_background et_pb_section_parallax et_section_regular" > <div class="et_pb_row et_pb_row_5 et_pb_equal_columns et_pb_gutters3"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_12 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_6 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2 style="text-align: center;">WHY THIS APPROACH</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_2 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div> </div> </div><div class="et_pb_row et_pb_row_6 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_with_border et_pb_column_1_4 et_pb_column et_pb_column_13 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_7 et_clickable et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p style="text-align: center;"><b>ICH: </b>Draft Guidline for Consultation Q5A (R2) viral safety evaluation of biotechnology products derived from cell lines of human or animal origin</p> <p style="text-align: center;"><a href="https://www.ema.europa.eu/en/documents/scientific-guideline/ich-q-5-r2-viral-safety-evaluation-biotechnology-products-derived-cell-lines-human-animal-origin_en.pdf" target="_blank" rel="noopener"><strong><span style="color: #249fda;">FIND OUT MORE</span></strong></a></p></div> </div> </div><div class="et_pb_with_border et_pb_column_1_4 et_pb_column et_pb_column_14 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_8 et_clickable et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p style="text-align: center;"><b>Publication:</b> Historical evaluation of the <i>in vivo </i>adventitious virus test and its potential for replacement with next generation sequencing (NGS)</p> <p style="text-align: center;"><a href="https://www.sciencedirect.com/science/article/pii/S104510562200080X" target="_blank" rel="noopener"><strong><span style="color: #249fda;">FIND OUT MORE</span></strong></a></p></div> </div> </div><div class="et_pb_with_border et_pb_column_1_4 et_pb_column et_pb_column_15 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_9 et_clickable et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p style="text-align: center;"><b>Publication:</b> Systematic evaluation of <i>in vitro </i>and <i>in vivo</i> adventitious virus assays for the detection of viral contamination of cell banks and biological products</p> <p style="text-align: center;"><a href="https://www.sciencedirect.com/science/article/abs/pii/S0264410X14001947" target="_blank" rel="noopener"><strong><span style="color: #249fda;">FIND OUT MORE</span></strong></a></p></div> </div> </div><div class="et_pb_with_border et_pb_column_1_4 et_pb_column et_pb_column_16 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_10 et_clickable et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p style="text-align: center;"><b>PathoQuest in vivo Brochure</b></p> <p style="text-align: center;"><a href="https://pathoquest.com/wp-content/uploads/2023/07/PATHOQUEST-BROCHURE.pdf" target="_blank" rel="noopener"><strong><span style="color: #249fda;">FIND OUT MORE</span></strong></a></p></div> </div> </div> </div> </div><div id="SR" class="et_pb_section et_pb_section_4 et_pb_section_parallax et_pb_with_background et_section_regular" > <span class="et_parallax_bg_wrap"><span class="et_parallax_bg" style="background-image: url(https://pathoquest.com/wp-content/uploads/2023/06/Swoosh_background.svg);" ></span></span> <div class="et_pb_row et_pb_row_7 et_pb_equal_columns et_pb_gutters3"> <div class="et_pb_column et_pb_column_1_3 et_pb_column_17 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_11 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2 style="text-align: left;">Our comparison data</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_3 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div><div class="et_pb_module et_pb_text et_pb_text_12 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><b>COMPARISON OF LIMITS OF DETECTION FOR </b><b><i>IN VIVO</i></b><b> AND NGS ASSAYS FOR MODEL ADVENTITIOUS VIRAL AGENTS.</b></p> <p><i>In vivo</i> assays were performed using suckling mice, adult mice, and embryonated hens’ eggs. For ethical purposes, we chose not to perform all <i>in vivo</i> tests. For each virus category (defined in this <a href="https://www.sciencedirect.com/science/article/abs/pii/S0264410X14001947" target="_blank" rel="noopener">publication</a>), only the most sensitive reported ones were used.</div> </div> </div><div class="et_pb_column et_pb_column_2_3 et_pb_column_18 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_image et_pb_image_4"> <span class="et_pb_image_wrap "><img loading="lazy" decoding="async" width="1610" height="816" src="https://pathoquest.com/wp-content/uploads/2023/07/Our_comparison_data_graphic.webp" alt="" title="Our_comparison_data_graphic" srcset="https://pathoquest.com/wp-content/uploads/2023/07/Our_comparison_data_graphic.webp 1610w, https://pathoquest.com/wp-content/uploads/2023/07/Our_comparison_data_graphic-1280x649.webp 1280w, https://pathoquest.com/wp-content/uploads/2023/07/Our_comparison_data_graphic-980x497.webp 980w, https://pathoquest.com/wp-content/uploads/2023/07/Our_comparison_data_graphic-480x243.webp 480w" sizes="(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) and (max-width: 980px) 980px, (min-width: 981px) and (max-width: 1280px) 1280px, (min-width: 1281px) 1610px, 100vw" class="wp-image-291" /></span> </div> </div> </div> </div><div id="CS" class="et_pb_section et_pb_section_5 et_pb_section_parallax et_pb_with_background et_section_regular" > <span class="et_parallax_bg_wrap"><span class="et_parallax_bg" style="background-image: url(https://pathoquest.com/wp-content/uploads/2023/06/dentity_confirmation_mid_banner.webp);" ></span></span> <div class="et_pb_row et_pb_row_8 et_pb_equal_columns"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_19 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_13 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2>Challenges solved</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_4 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div> </div> </div><div class="et_pb_row et_pb_row_9 et_pb_equal_columns"> <div class="et_pb_column et_pb_column_1_2 et_pb_column_20 vertical-align et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_text et_pb_text_14 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><ul> <li><span style="color: #ffffff;">Difficulty in generating a neutralizing antibody</span></li> <li><span style="color: #ffffff;">Incompatibility of test material with <i>in vivo </i>methods</span></li> <li><span style="color: #ffffff;">Limited sample volume for <i>in vivo </i>characterization</span></li> <li><span style="color: #ffffff;">Meeting corporate objectives to reduce animal use</span></li> </ul></div> </div><div class="et_pb_button_module_wrapper et_pb_button_7_wrapper et_pb_button_alignment_left et_pb_module "> <a class="et_pb_button et_pb_button_7 et_pb_bg_layout_light" href="https://pathoquest.com/wp-content/uploads/2023/07/PATHOQUEST-INVIVO-BROCHURE_A4-OK.pdf" target="_blank">Download brochure </a> </div> </div><div class="et_pb_column et_pb_column_1_2 et_pb_column_21 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_slider et_pb_slider_0 et_pb_slider_fullwidth_off"> <div class="et_pb_slides"> <div class="et_pb_slide et_pb_slide_0 et_pb_bg_layout_dark et_pb_media_alignment_center et-pb-active-slide" data-slide-id="et_pb_slide_0"> <div class="et_pb_container clearfix"> <div class="et_pb_slider_container_inner"> <div class="et_pb_slide_description"> <div class="et_pb_slide_content"><p style="text-align: left;">“Due to the nature of our therapeutic it was not possible to generate a neutralizing antibody. The use of <i>in vivo </i>assays would therefore have been challenging as we anticipated adverse effects within the tests. PathoQuest was able to provide us an NGS based alternative characterization strategy which we were able to present to the regulators as a viable alternative to traditional <i>in vivo </i>methods.”</p></div> </div> </div> </div> </div> </div> </div> <div class="et_pb_button_module_wrapper et_pb_button_8_wrapper et_pb_button_alignment_center et_pb_module "> <a class="et_pb_button et_pb_button_8 et_pb_bg_layout_light" href="#contact">CONTACT US</a> </div> </div> </div> </div><div id="OS" class="et_pb_section et_pb_section_6 et_section_regular" > <div class="et_pb_row et_pb_row_10 et_pb_equal_columns et_pb_gutters3"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_22 vertical-align et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_15 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2 style="text-align: center;">OTHER SERVICES</h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_5 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div> </div> </div><div class="et_pb_row et_pb_row_11 et_pb_equal_columns et_pb_gutters1"> <div class="et_pb_column et_pb_column_1_3 et_pb_column_23 et_clickable et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_image et_pb_image_5"> <span class="et_pb_image_wrap "><img decoding="async" src="https://pathoquest.com/wp-content/uploads/2023/04/Adventitious-Virus.svg" alt="" title="_Adventitious Virus" class="wp-image-30" /></span> </div><div class="et_pb_module et_pb_text et_pb_text_16 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h3 style="text-align: center;">Adventitious Virus Testing</h3> <p style="text-align: center;">Detection of viral contamination within the manufacturing process and beyond.</p> <p style="text-align: center;"><strong><span style="color: #249fda;">READ MORE</span></strong></p></div> </div> </div><div class="et_pb_column 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MORE</span></strong></p></div> </div> </div> </div><div class="et_pb_row et_pb_row_12 et_pb_equal_columns et_pb_gutters2"> <div class="et_pb_column et_pb_column_1_3 et_pb_column_26 et_clickable et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_image et_pb_image_8"> <span class="et_pb_image_wrap "><img decoding="async" src="https://pathoquest.com/wp-content/uploads/2023/04/Cell-Line-Characterisation.svg" alt="" title="_Cell Line Characterisation" class="wp-image-31" /></span> </div><div class="et_pb_module et_pb_text et_pb_text_19 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h3 style="text-align: center;">Cell Line Characterization</h3> <p style="text-align: center;">Biosafety screening and stability testing of manufacturing cells.</p> <p style="text-align: center;"><strong><span style="color: #249fda;">READ MORE</span></strong></p></div> </div> </div><div class="et_pb_column et_pb_column_1_3 et_pb_column_27 et_clickable et_pb_css_mix_blend_mode_passthrough"> <div class="et_pb_module et_pb_image et_pb_image_9"> <span class="et_pb_image_wrap "><img decoding="async" src="https://pathoquest.com/wp-content/uploads/2023/07/HLA-Genotyping.svg" alt="" title="_HLA Genotyping" class="wp-image-284" /></span> </div><div class="et_pb_module et_pb_text et_pb_text_20 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h3 style="text-align: center;">HLA Genotyping</h3> <p style="text-align: center;">Characterizing and screening for novel and emerging cell therapies.</p> <p style="text-align: center;"><strong><span style="color: #249fda;">READ MORE</span></strong></p></div> </div> </div><div class="et_pb_column et_pb_column_1_3 et_pb_column_28 et_clickable et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_image et_pb_image_10"> <span class="et_pb_image_wrap "><img decoding="async" src="https://pathoquest.com/wp-content/uploads/2023/04/Raw-material-Testing.svg" 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Read our Privacy Notice for more information.<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></legend><div class='ginput_container ginput_container_consent'><input name='input_6.1' id='input_1_6_1' type='checkbox' value='1' aria-required="true" aria-invalid="false" /> <label class="gform-field-label gform-field-label--type-inline gfield_consent_label" for='input_1_6_1' >I agree to the privacy policy.</label><input type='hidden' name='input_6.2' value='I agree to the privacy policy.' class='gform_hidden' /><input type='hidden' name='input_6.3' value='2' class='gform_hidden' /></div></fieldset><div id="field_1_9" class="gfield gfield--type-honeypot gform_validation_container field_sublabel_above gfield--has-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_9" ><label class='gfield_label gform-field-label' for='input_1_9'>Email</label><div class='gfield_description' id='gfield_description_1_9'>This field is for validation purposes and should be left unchanged.</div><div class='ginput_container'><input name='input_9' id='input_1_9' type='text' value='' autocomplete='new-password'/></div></div></div></div> <div class='gform_footer top_label'> <input type='submit' id='gform_submit_button_1' class='gform_button button et_pb_button' value='Submit' onclick='if(window["gf_submitting_1"]){return false;} if( !jQuery("#gform_1")[0].checkValidity || jQuery("#gform_1")[0].checkValidity()){window["gf_submitting_1"]=true;} ' onkeypress='if( event.keyCode == 13 ){ if(window["gf_submitting_1"]){return false;} if( !jQuery("#gform_1")[0].checkValidity || jQuery("#gform_1")[0].checkValidity()){window["gf_submitting_1"]=true;} jQuery("#gform_1").trigger("submit",[true]); }' /> <input type='hidden' name='gform_ajax' value='form_id=1&amp;title=&amp;description=&amp;tabindex=0&amp;theme=gravity-theme' /> <input type='hidden' class='gform_hidden' name='is_submit_1' value='1' /> <input type='hidden' class='gform_hidden' name='gform_submit' value='1' /> <input type='hidden' class='gform_hidden' name='gform_unique_id' value='' /> <input type='hidden' class='gform_hidden' name='state_1' value='WyJ7XCI2LjFcIjpcImI5ZDIyMDQ1YjdjYTViMTQ1NzYwODc1NmY3ZjRlMGYyXCIsXCI2LjJcIjpcIjJhYTFhOGQ0NDNiMTFjY2YzOGNlNTAyNmNlZmQ4ZWUxXCIsXCI2LjNcIjpcIjVlM2YzZThhYjhlOWZjZDU4YzVlNTc0ZmM4NTM0OWVmXCJ9IiwiYTc2ZjZhMzE2MTIzZTZjZThjZGQyOTM2ZWFmNGUwYzciXQ==' /> <input type='hidden' class='gform_hidden' name='gform_target_page_number_1' id='gform_target_page_number_1' value='0' /> <input type='hidden' class='gform_hidden' name='gform_source_page_number_1' id='gform_source_page_number_1' value='1' /> <input type='hidden' name='gform_field_values' value='' /> </div> <input type="hidden" id="ct_checkjs_68264bdb65b97eeae6788aa3348e553c" name="ct_checkjs" value="0" /><script >setTimeout(function(){var ct_input_name = "ct_checkjs_68264bdb65b97eeae6788aa3348e553c";if (document.getElementById(ct_input_name) !== null) {var ct_input_value = document.getElementById(ct_input_name).value;document.getElementById(ct_input_name).value = document.getElementById(ct_input_name).value.replace(ct_input_value, '2085491878');}}, 1000);</script><label class="apbct_special_field" id="apbct_label_id56063" for="apbct__email_id__gravity_form_56063">56063</label><input id="apbct__email_id__gravity_form_56063" class="apbct_special_field apbct__email_id__gravity_form" autocomplete="off" name="apbct__email_id__gravity_form_56063" type="text" value="56063" size="30" apbct_event_id="56063" maxlength="200" /><input id="apbct_event_id" class="apbct_special_field" name="apbct_event_id" type="hidden" value="56063" /></form> </div> <iframe style='display:none;width:0px;height:0px;' src='about:blank' name='gform_ajax_frame_1' id='gform_ajax_frame_1' title='This iframe contains the logic required to handle Ajax powered Gravity Forms.'></iframe> <script type="text/javascript"> /* <![CDATA[ */ gform.initializeOnLoaded( function() {gformInitSpinner( 1, 'https://pathoquest.com/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery('#gform_ajax_frame_1').on('load',function(){var contents = jQuery(this).contents().find('*').html();var is_postback = contents.indexOf('GF_AJAX_POSTBACK') >= 0;if(!is_postback){return;}var form_content = jQuery(this).contents().find('#gform_wrapper_1');var is_confirmation = jQuery(this).contents().find('#gform_confirmation_wrapper_1').length > 0;var is_redirect = contents.indexOf('gformRedirect(){') >= 0;var is_form = form_content.length > 0 && ! is_redirect && ! is_confirmation;var mt = parseInt(jQuery('html').css('margin-top'), 10) + parseInt(jQuery('body').css('margin-top'), 10) + 100;if(is_form){jQuery('#gform_wrapper_1').html(form_content.html());if(form_content.hasClass('gform_validation_error')){jQuery('#gform_wrapper_1').addClass('gform_validation_error');} else {jQuery('#gform_wrapper_1').removeClass('gform_validation_error');}setTimeout( function() { /* delay the scroll by 50 milliseconds to fix a bug in chrome */ jQuery(document).scrollTop(jQuery('#gform_wrapper_1').offset().top - mt); }, 50 );if(window['gformInitDatepicker']) {gformInitDatepicker();}if(window['gformInitPriceFields']) {gformInitPriceFields();}var current_page = jQuery('#gform_source_page_number_1').val();gformInitSpinner( 1, 'https://pathoquest.com/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery(document).trigger('gform_page_loaded', [1, current_page]);window['gf_submitting_1'] = false;}else if(!is_redirect){var confirmation_content = jQuery(this).contents().find('.GF_AJAX_POSTBACK').html();if(!confirmation_content){confirmation_content = contents;}jQuery('#gform_wrapper_1').replaceWith(confirmation_content);jQuery(document).scrollTop(jQuery('#gf_1').offset().top - mt);jQuery(document).trigger('gform_confirmation_loaded', [1]);window['gf_submitting_1'] = false;wp.a11y.speak(jQuery('#gform_confirmation_message_1').text());}else{jQuery('#gform_1').append(contents);if(window['gformRedirect']) {gformRedirect();}}jQuery(document).trigger("gform_pre_post_render", [{ formId: "1", currentPage: "current_page", abort: function() { this.preventDefault(); } }]); if (event && event.defaultPrevented) { return; } const gformWrapperDiv = document.getElementById( "gform_wrapper_1" ); if ( gformWrapperDiv ) { const visibilitySpan = document.createElement( "span" ); visibilitySpan.id = "gform_visibility_test_1"; gformWrapperDiv.insertAdjacentElement( "afterend", visibilitySpan ); } const visibilityTestDiv = document.getElementById( "gform_visibility_test_1" ); let postRenderFired = false; function triggerPostRender() { if ( postRenderFired ) { return; } postRenderFired = true; jQuery( document ).trigger( 'gform_post_render', [1, current_page] ); gform.utils.trigger( { event: 'gform/postRender', native: false, data: { formId: 1, currentPage: current_page } } ); if ( visibilityTestDiv ) { visibilityTestDiv.parentNode.removeChild( visibilityTestDiv ); } } function debounce( func, wait, immediate ) { var timeout; return function() { var context = this, args = arguments; var later = function() { timeout = null; if ( !immediate ) func.apply( context, args ); }; var callNow = immediate && !timeout; clearTimeout( timeout ); timeout = setTimeout( later, wait ); if ( callNow ) func.apply( context, args ); }; } const debouncedTriggerPostRender = debounce( function() { triggerPostRender(); }, 200 ); if ( visibilityTestDiv && visibilityTestDiv.offsetParent === null ) { const observer = new MutationObserver( ( mutations ) => { mutations.forEach( ( mutation ) => { if ( mutation.type === 'attributes' && visibilityTestDiv.offsetParent !== null ) { debouncedTriggerPostRender(); observer.disconnect(); } }); }); observer.observe( document.body, { attributes: true, childList: false, subtree: true, attributeFilter: [ 'style', 'class' ], }); } else { triggerPostRender(); } } );} ); /* ]]&gt; */ </script> </section> </div> </div> </div> </div><div class="et_pb_row et_pb_row_15 et_pb_equal_columns et_pb_gutters1"> <div class="et_pb_column et_pb_column_4_4 et_pb_column_34 et_pb_css_mix_blend_mode_passthrough et-last-child"> <div class="et_pb_module et_pb_text et_pb_text_29 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2><span style="color: #ffffff;"><span class="ui-provider bra brb c d e f g h i j k l m n o p q r s t brc brd w x y z ab ac ae af ag ah ai aj ak" dir="ltr">U.S.</span></span></h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_8 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div><div class="et_pb_module et_pb_text et_pb_text_30 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p><span class="ui-provider bra brb c d e f g h i j k l m n o p q r s t brc brd w x y z ab ac ae af ag ah ai aj ak" dir="ltr" style="color: #ffffff;"><a rel="noreferrer noopener" title="tel:+14842129360" target="_blank" style="color: #ffffff;">+1 484 212 9360</a></span></p> <p><span style="color: #ffffff;">466 Devon Park Dr<br />Wayne, PA 19087<br />United States</span></p></div> </div><div class="et_pb_module et_pb_text et_pb_text_31 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><h2><span style="color: #ffffff;"><span class="ui-provider bra brb c d e f g h i j k l m n o p q r s t brc brd w x y z ab ac ae af ag ah ai aj ak" dir="ltr">France</span></span></h2></div> </div><div class="et_pb_module et_pb_divider et_pb_divider_9 et_pb_divider_position_center et_pb_space"><div class="et_pb_divider_internal"></div></div><div class="et_pb_module et_pb_text et_pb_text_32 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p><span style="color: #ffffff;">+33 (0)1 70 82 17 90</span></p> <p><span style="color: #ffffff;">Biopark -Bâtiment B,<br />11, rue Watt<br />75013 Paris, France</span></p></div> </div><div class="et_pb_module et_pb_text et_pb_text_33 et_pb_text_align_left et_pb_bg_layout_light"> <div class="et_pb_text_inner"><p><strong>How can PathoQuest help?</strong></p></div> </div><div class="et_pb_module et_pb_wpt_gravityform et_pb_wpt_gravityform_1"> <div class="et_pb_module_inner"> <section class='et_pb_module et_pb_wpt_gravityform et_pb_wpt_gravityform_1' data-class='et_pb_module et_pb_wpt_gravityform et_pb_wpt_gravityform_1' id="" data-type='et_pb_wpt_gravityform' data-is-sticky='no'> <div class='gf_browser_gecko gform_wrapper gravity-theme gform-theme--no-framework' data-form-theme='gravity-theme' data-form-index='1' id='gform_wrapper_1' ><form method='post' enctype='multipart/form-data' id='gform_1' action='/services/in-vivo-replacement/' data-formid='1' novalidate> <input type='hidden' class='gforms-pum' value='{"closepopup":false,"closedelay":0,"openpopup":false,"openpopup_id":0}' /> <div class='gform-body gform_body'><div id='gform_fields_1' class='gform_fields top_label form_sublabel_above description_above validation_below'><fieldset id="field_1_1" class="gfield gfield--type-name gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_1" ><legend class='gfield_label gform-field-label gfield_label_before_complex' >Name<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></legend><div class='ginput_complex ginput_container ginput_container--name no_prefix has_first_name no_middle_name has_last_name no_suffix gf_name_has_2 ginput_container_name gform-grid-row' id='input_1_1'> <span id='input_1_1_3_container' class='name_first gform-grid-col gform-grid-col--size-auto' > <label for='input_1_1_3' class='gform-field-label gform-field-label--type-sub '>First</label> <input type='text' name='input_1.3' id='input_1_1_3' value='' aria-required='true' autocomplete="given-name" /> </span> <span id='input_1_1_6_container' class='name_last gform-grid-col gform-grid-col--size-auto' > <label for='input_1_1_6' class='gform-field-label gform-field-label--type-sub '>Last</label> <input type='text' name='input_1.6' id='input_1_1_6' value='' aria-required='true' autocomplete="family-name" /> </span> </div></fieldset><div id="field_1_2" class="gfield gfield--type-email gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_2" ><label class='gfield_label gform-field-label' for='input_1_2'>Email<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></label><div class='ginput_container ginput_container_email'> <input name='input_2' id='input_1_2' type='email' value='' class='large' aria-required="true" aria-invalid="false" /> </div></div><div id="field_1_4" class="gfield gfield--type-text gfield--width-full gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_4" ><label class='gfield_label gform-field-label' for='input_1_4'>Company<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></label><div class='ginput_container ginput_container_text'><input name='input_4' id='input_1_4' type='text' value='' class='large' aria-required="true" aria-invalid="false" /> </div></div><div id="field_1_8" class="gfield gfield--type-text gfield--width-full gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_8" ><label class='gfield_label gform-field-label' for='input_1_8'>Country<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></label><div class='ginput_container ginput_container_text'><input name='input_8' id='input_1_8' type='text' value='' class='large' aria-required="true" aria-invalid="false" /> </div></div><div id="field_1_3" class="gfield gfield--type-textarea gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_3" ><label class='gfield_label gform-field-label' for='input_1_3'>Tell us more about your needs and projects<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></label><div class='ginput_container ginput_container_textarea'><textarea name='input_3' id='input_1_3' class='textarea medium' maxlength='600' aria-required="true" aria-invalid="false" rows='10' cols='50'></textarea></div></div><fieldset id="field_1_6" class="gfield gfield--type-consent gfield--type-choice gfield--input-type-consent gfield_contains_required field_sublabel_above gfield--no-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_6" ><legend class='gfield_label gform-field-label gfield_label_before_complex' >By submitting this form, PathoQuest will use the personal data you provide to handle your request. Read our Privacy Notice for more information.<span class="gfield_required"><span class="gfield_required gfield_required_text">(Required)</span></span></legend><div class='ginput_container ginput_container_consent'><input name='input_6.1' id='input_1_6_1' type='checkbox' value='1' aria-required="true" aria-invalid="false" /> <label class="gform-field-label gform-field-label--type-inline gfield_consent_label" for='input_1_6_1' >I agree to the privacy policy.</label><input type='hidden' name='input_6.2' value='I agree to the privacy policy.' class='gform_hidden' /><input type='hidden' name='input_6.3' value='2' class='gform_hidden' /></div></fieldset><div id="field_1_9" class="gfield gfield--type-honeypot gform_validation_container field_sublabel_above gfield--has-description field_description_above field_validation_below gfield_visibility_visible" data-js-reload="field_1_9" ><label class='gfield_label gform-field-label' for='input_1_9'>Email</label><div class='gfield_description' id='gfield_description_1_9'>This field is for validation purposes and should be left unchanged.</div><div class='ginput_container'><input name='input_9' id='input_1_9' type='text' value='' autocomplete='new-password'/></div></div></div></div> <div class='gform_footer top_label'> <input type='submit' id='gform_submit_button_1' class='gform_button button et_pb_button' value='Submit' onclick='if(window["gf_submitting_1"]){return false;} if( !jQuery("#gform_1")[0].checkValidity || jQuery("#gform_1")[0].checkValidity()){window["gf_submitting_1"]=true;} ' onkeypress='if( event.keyCode == 13 ){ if(window["gf_submitting_1"]){return false;} if( !jQuery("#gform_1")[0].checkValidity || jQuery("#gform_1")[0].checkValidity()){window["gf_submitting_1"]=true;} jQuery("#gform_1").trigger("submit",[true]); }' /> <input type='hidden' class='gform_hidden' name='is_submit_1' value='1' /> <input type='hidden' class='gform_hidden' name='gform_submit' value='1' /> <input type='hidden' class='gform_hidden' name='gform_unique_id' value='' /> <input type='hidden' class='gform_hidden' name='state_1' value='WyJ7XCI2LjFcIjpcImI5ZDIyMDQ1YjdjYTViMTQ1NzYwODc1NmY3ZjRlMGYyXCIsXCI2LjJcIjpcIjJhYTFhOGQ0NDNiMTFjY2YzOGNlNTAyNmNlZmQ4ZWUxXCIsXCI2LjNcIjpcIjVlM2YzZThhYjhlOWZjZDU4YzVlNTc0ZmM4NTM0OWVmXCJ9IiwiYTc2ZjZhMzE2MTIzZTZjZThjZGQyOTM2ZWFmNGUwYzciXQ==' /> <input type='hidden' class='gform_hidden' name='gform_target_page_number_1' id='gform_target_page_number_1' value='0' /> <input type='hidden' class='gform_hidden' name='gform_source_page_number_1' id='gform_source_page_number_1' value='1' /> <input type='hidden' name='gform_field_values' value='' /> </div> <input type="hidden" id="ct_checkjs_c2626d850c80ea07e7511bbae4c76f4b" name="ct_checkjs" value="0" /><script >setTimeout(function(){var ct_input_name = "ct_checkjs_c2626d850c80ea07e7511bbae4c76f4b";if (document.getElementById(ct_input_name) !== null) {var ct_input_value = document.getElementById(ct_input_name).value;document.getElementById(ct_input_name).value = document.getElementById(ct_input_name).value.replace(ct_input_value, '2085491878');}}, 1000);</script><label class="apbct_special_field" id="apbct_label_id29688" for="apbct__email_id__gravity_form_29688">29688</label><input id="apbct__email_id__gravity_form_29688" class="apbct_special_field apbct__email_id__gravity_form" autocomplete="off" name="apbct__email_id__gravity_form_29688" type="text" value="29688" size="30" apbct_event_id="29688" maxlength="200" /><input id="apbct_event_id" class="apbct_special_field" name="apbct_event_id" type="hidden" value="29688" /></form> </div><script type="text/javascript"> /* <![CDATA[ */ gform.initializeOnLoaded( function() {gformInitSpinner( 1, 'https://pathoquest.com/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery('#gform_ajax_frame_1').on('load',function(){var contents = jQuery(this).contents().find('*').html();var is_postback = contents.indexOf('GF_AJAX_POSTBACK') >= 0;if(!is_postback){return;}var form_content = jQuery(this).contents().find('#gform_wrapper_1');var is_confirmation = jQuery(this).contents().find('#gform_confirmation_wrapper_1').length > 0;var is_redirect = contents.indexOf('gformRedirect(){') >= 0;var is_form = form_content.length > 0 && ! is_redirect && ! is_confirmation;var mt = parseInt(jQuery('html').css('margin-top'), 10) + parseInt(jQuery('body').css('margin-top'), 10) + 100;if(is_form){jQuery('#gform_wrapper_1').html(form_content.html());if(form_content.hasClass('gform_validation_error')){jQuery('#gform_wrapper_1').addClass('gform_validation_error');} else {jQuery('#gform_wrapper_1').removeClass('gform_validation_error');}setTimeout( function() { /* delay the scroll by 50 milliseconds to fix a bug in chrome */ }, 50 );if(window['gformInitDatepicker']) {gformInitDatepicker();}if(window['gformInitPriceFields']) {gformInitPriceFields();}var current_page = jQuery('#gform_source_page_number_1').val();gformInitSpinner( 1, 'https://pathoquest.com/wp-content/plugins/gravityforms/images/spinner.svg', true );jQuery(document).trigger('gform_page_loaded', [1, current_page]);window['gf_submitting_1'] = false;}else if(!is_redirect){var confirmation_content = jQuery(this).contents().find('.GF_AJAX_POSTBACK').html();if(!confirmation_content){confirmation_content = contents;}jQuery('#gform_wrapper_1').replaceWith(confirmation_content);jQuery(document).trigger('gform_confirmation_loaded', [1]);window['gf_submitting_1'] = false;wp.a11y.speak(jQuery('#gform_confirmation_message_1').text());}else{jQuery('#gform_1').append(contents);if(window['gformRedirect']) {gformRedirect();}}jQuery(document).trigger("gform_pre_post_render", [{ formId: "1", currentPage: "current_page", abort: function() { this.preventDefault(); } }]); if (event && event.defaultPrevented) { return; } const gformWrapperDiv = document.getElementById( "gform_wrapper_1" ); if ( gformWrapperDiv ) { const visibilitySpan = document.createElement( "span" ); visibilitySpan.id = "gform_visibility_test_1"; gformWrapperDiv.insertAdjacentElement( "afterend", visibilitySpan ); } const visibilityTestDiv = document.getElementById( "gform_visibility_test_1" ); let postRenderFired = false; function triggerPostRender() { if ( postRenderFired ) { return; } postRenderFired = true; jQuery( document ).trigger( 'gform_post_render', [1, current_page] ); gform.utils.trigger( { event: 'gform/postRender', native: false, data: { formId: 1, currentPage: current_page } } ); if ( visibilityTestDiv ) { visibilityTestDiv.parentNode.removeChild( visibilityTestDiv ); } } function debounce( func, wait, immediate ) { var timeout; return function() { var context = this, args = arguments; var later = function() { timeout = null; if ( !immediate ) func.apply( context, args ); 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