CINXE.COM

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class="block block-system"> <div class="content"> <div class="term-listing-heading"><div id="taxonomy-term-27916" class="taxonomy-term vocabulary-protocol-types"> <div class="content"> </div> </div> </div><div id="node-120031" class="node node-protocols node-teaser clearfix" about="/resources/protocols/rat-cortical-stem-cell-culture-expansion-protocol" typeof="sioc:Item foaf:Document"> <h2><a href="/resources/protocols/rat-cortical-stem-cell-culture-expansion-protocol">Rat Cortical Stem Cell Culture Expansion Protocol</a></h2> <span property="dc:title" content="Rat Cortical Stem Cell Culture Expansion Protocol" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p><strong>This protocol must be read in its entirety before using Catalog # NSC001.</strong> <strong>Caution:</strong> The <a href="/products/rat-cortical-stem-cells-3-x-10e6-cells-vial-_nsc001">rat cortical stem cells</a> used in this protocol contain trace amounts of human transferrin and DMSO, and the media used in this protocol contains trace amounts of transferrin. The transferrin was purified from donor plasma and tested at the donor level using an FDA licensed method and found to be non-reactive for anti HIV-1/2 and Hepatitis B surface antigen.</p></div></div></div> </div> <ul class="links inline"><li class="node-readmore first last"><a href="/resources/protocols/rat-cortical-stem-cell-culture-expansion-protocol" rel="tag" title="Rat Cortical Stem Cell Culture Expansion Protocol">Read more<span class="element-invisible"> about Rat Cortical Stem Cell Culture Expansion Protocol</span></a></li> </ul> </div> <div id="node-119741" class="node node-protocols node-teaser clearfix" about="/resources/protocols/mouse-hematopoietic-lineage-depletion" typeof="sioc:Item foaf:Document"> <h2><a href="/resources/protocols/mouse-hematopoietic-lineage-depletion">Mouse Hematopoietic Lineage Depletion</a></h2> <span property="dc:title" content="Mouse Hematopoietic Lineage Depletion" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p>Antibodies including anti-mouse CD5, CD11b, CD45R (B220), Ly-6G (Gr-1), and TER-119 can be used to efficiently tag bone marrow-derived cells committed to major hematopoietic lineages, including T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, and erythrocytes. These antibodies can be used in conjunction with magnetic particle separation systems or flow cytometric cell sorting to deplete lineage-committed cells and enrich for uncommitted hematopoietic progenitors.</p></div></div></div> </div> <ul class="links inline"><li class="node-readmore first last"><a href="/resources/protocols/mouse-hematopoietic-lineage-depletion" rel="tag" title="Mouse Hematopoietic Lineage Depletion">Read more<span class="element-invisible"> about Mouse Hematopoietic Lineage Depletion</span></a></li> </ul> </div> <div id="node-5081" class="node node-protocols node-teaser clearfix" about="/resources/protocols/culturing-rat-cortical-stem-cells-expansion-using-monolayer-system" typeof="sioc:Item foaf:Document"> <h2><a href="/resources/protocols/culturing-rat-cortical-stem-cells-expansion-using-monolayer-system">Culturing Rat Cortical Stem Cells: Expansion using the Monolayer System</a></h2> <span property="dc:title" content="Culturing Rat Cortical Stem Cells: Expansion using the Monolayer System" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p><em>Ex vivo</em> expanded neural stem cells serve as excellent tools for researchers studying neural development and neurological disorders. Ready-to-use <a href="/products/rat-cortical-stem-cells-3-x-10e6-cells-vial-_nsc001">primary cortical stem cells</a>, isolated from E14.5 Sprague-Dawley rats (Catalog # NSC001), can be grown in monolayer, as described here, or as neurospheres. These cells retain capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes.<br /> Please read the protocol in its entirety before starting.</p></div></div></div> </div> <ul class="links inline"><li class="node-readmore first last"><a href="/resources/protocols/culturing-rat-cortical-stem-cells-expansion-using-monolayer-system" rel="tag" title="Culturing Rat Cortical Stem Cells: Expansion using the Monolayer System">Read more<span class="element-invisible"> about Culturing Rat Cortical Stem Cells: Expansion using the Monolayer System</span></a></li> </ul> </div> <div id="node-3471" class="node node-protocols node-teaser clearfix" about="/resources/protocols/definitive-endoderm-differentiation-bg01v-human-embryonic-stem-cells" typeof="sioc:Item foaf:Document"> <h2><a href="/resources/protocols/definitive-endoderm-differentiation-bg01v-human-embryonic-stem-cells">Definitive Endoderm Differentiation of BG01V Human Embryonic Stem Cells</a></h2> <span property="dc:title" content="Definitive Endoderm Differentiation of BG01V Human Embryonic Stem Cells" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p><em>This protocol is designed for BG01V human embryonic stem (hES) cells grown in <a href="/products/mouse-embryonic-fibroblast-mef-conditioned-media_ar005">Mouse Embryonic Fibroblast (MEF) Conditioned Media</a> (Catalog # AR005). If using different cell lines or growth media, the protocol below may need to be modified.</em></p> <p><em>The quality of the human pluripotent cells used in the differentiation is critical. Use of suboptimal quality or very high passage pluripotent cells can result in decreased differentiation efficiency and/or increased cell death.</em></p></div></div></div> </div> <ul class="links inline"><li class="node-readmore first last"><a href="/resources/protocols/definitive-endoderm-differentiation-bg01v-human-embryonic-stem-cells" rel="tag" title="Definitive Endoderm Differentiation of BG01V Human Embryonic Stem Cells">Read more<span class="element-invisible"> about Definitive Endoderm Differentiation of BG01V Human Embryonic Stem Cells</span></a></li> </ul> </div> <div id="node-2876" class="node node-protocols node-teaser clearfix" about="/resources/technical/endoderm-ectoderm-and-mesoderm-differentiation-bg01v-human-embryonic-stem-cells" typeof="sioc:Item foaf:Document"> <h2><a href="/resources/technical/endoderm-ectoderm-and-mesoderm-differentiation-bg01v-human-embryonic-stem-cells">Endoderm, Ectoderm, and Mesoderm Differentiation of BG01V Human Embryonic Stem Cells</a></h2> <span property="dc:title" content="Endoderm, Ectoderm, and Mesoderm Differentiation of BG01V Human Embryonic Stem Cells" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p><em>This protocol is designed for BG01V human embryonic stem (hES) cells grown in <a href="/products/mouse-embryonic-fibroblast-mef-conditioned-media_ar005">Mouse Embryonic Fibroblast (MEF) Conditioned Media</a> (Catalog # AR005). If using different cell lines or growth media, the protocol below may need to be modified.</em></p> <p><em>The quality of the human pluripotent cells used in the differentiation is imperative. Use of suboptimal quality or very high passage pluripotent cells can result in decreased differentiation efficiency and/or increased cell death.</em></p></div></div></div> </div> <ul class="links inline"><li class="node-readmore first last"><a href="/resources/technical/endoderm-ectoderm-and-mesoderm-differentiation-bg01v-human-embryonic-stem-cells" rel="tag" title="Endoderm, Ectoderm, and Mesoderm Differentiation of BG01V Human Embryonic Stem Cells">Read more<span class="element-invisible"> about Endoderm, Ectoderm, and Mesoderm Differentiation of BG01V Human Embryonic Stem Cells</span></a></li> </ul> </div> <div id="node-2856" class="node node-protocols node-teaser clearfix" about="/resources/protocols/chondrogenic-differentiation-humanmouse-mesenchymal-stem-cells" typeof="sioc:Item foaf:Document"> <h2><a href="/resources/protocols/chondrogenic-differentiation-humanmouse-mesenchymal-stem-cells">Chondrogenic Differentiation of Human/Mouse Mesenchymal Stem Cells</a></h2> <span property="dc:title" content="Chondrogenic Differentiation of Human/Mouse Mesenchymal Stem Cells" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p>Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into multiple cell types including adipocytes, chondrocytes and osteocytes. This protocol describes the chondrogenic differentiation of MSCs using the <a href="/products/stemxvivo-chondrogenic-base-media_ccm005">StemXVivo<sup>TM</sup> Human/Mouse Chondrogenic Base Media</a> (Catalog # CCM005) and <a href="/products/stemxvivo-human-mouse-chondrogenic-supplement-05-ml_ccm006">StemXVivo Human/Mouse Chondrogenic Supplement</a> (Catalog # CCM006).</p></div></div></div> </div> <ul class="links inline"><li class="node-readmore first last"><a href="/resources/protocols/chondrogenic-differentiation-humanmouse-mesenchymal-stem-cells" rel="tag" title="Chondrogenic Differentiation of Human/Mouse Mesenchymal Stem Cells">Read more<span class="element-invisible"> about Chondrogenic Differentiation of Human/Mouse Mesenchymal Stem Cells</span></a></li> </ul> </div> <div id="node-2841" class="node node-protocols node-teaser clearfix" about="/resources/protocols/human-colony-forming-cell-cfc-assay-using-methylcellulose-based-media" typeof="sioc:Item foaf:Document"> <h2><a href="/resources/protocols/human-colony-forming-cell-cfc-assay-using-methylcellulose-based-media">The Human Colony Forming Cell (CFC) Assay using Methylcellulose-based Media</a></h2> <span property="dc:title" content="The Human Colony Forming Cell (CFC) Assay using Methylcellulose-based Media" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p>The colony forming cell (CFC) assay, also referred to as the methylcellulose assay, is an <em>in vitro</em> assay used in the study of hematopoietic stem cells. The assay is based on the ability of hematopoietic progenitors to proliferate and differentiate into colonies in a semi-solid media in response to cytokine stimulation. The colonies formed can be enumerated and characterized according to their unique morphology.</p> <p>Please read the protocol in its entirety before starting.</p></div></div></div> </div> <ul class="links inline"><li class="node-readmore first last"><a href="/resources/protocols/human-colony-forming-cell-cfc-assay-using-methylcellulose-based-media" rel="tag" title="The Human Colony Forming Cell (CFC) Assay using Methylcellulose-based Media">Read more<span class="element-invisible"> about The Human Colony Forming Cell (CFC) Assay using Methylcellulose-based Media</span></a></li> </ul> </div> <div id="node-2826" class="node node-protocols node-teaser clearfix" about="/resources/protocols/procedure-serum-free-expansion-human-mesenchymal-stem-cells" typeof="sioc:Item foaf:Document"> <h2><a href="/resources/protocols/procedure-serum-free-expansion-human-mesenchymal-stem-cells">Procedure for Serum-Free Expansion of Human Mesenchymal Stem Cells</a></h2> <span property="dc:title" content="Procedure for Serum-Free Expansion of Human Mesenchymal Stem Cells" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p>Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into multiple cell types including adipocytes, chondrocytes, and osteocytes (1, 2). MSCs are phenotypically characterized as CD105<sup>+</sup>, CD90<sup>+</sup>, CD73<sup>+</sup>, CD44<sup>+</sup>, CD34<sup>-</sup>, CD45<sup>-</sup>, and CD11b<sup>-</sup> cells.</p></div></div></div> </div> <ul class="links inline"><li class="node-readmore first last"><a href="/resources/protocols/procedure-serum-free-expansion-human-mesenchymal-stem-cells" rel="tag" title="Procedure for Serum-Free Expansion of Human Mesenchymal Stem Cells">Read more<span class="element-invisible"> about Procedure for Serum-Free Expansion of Human Mesenchymal Stem Cells</span></a></li> </ul> </div> <div id="node-2821" class="node node-protocols node-teaser clearfix" about="/resources/protocols/adipogenic-differentiation-humanmouse-mesenchymal-stem-cells" typeof="sioc:Item foaf:Document"> <h2><a href="/resources/protocols/adipogenic-differentiation-humanmouse-mesenchymal-stem-cells">Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells</a></h2> <span property="dc:title" content="Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p>Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into multiple cell types including adipocytes, chondrocytes, and osteocytes. This protocol describes the adipogenic differentiation of human and mouse MSCs using the <a href="/products/stemxvivo-osteogenic-adipogenic-base-media_ccm007">StemXVivo™ Human/Mouse Osteogenic/Adipogenic Base Media</a> (Catalog # CCM007) and <a href="/products/stemxvivo-adipogenic-supplement-100x-_ccm011">StemXVivo Adipogenic Supplement</a> (Catalog # CCM011).</p></div></div></div> </div> <ul class="links inline"><li class="node-readmore first last"><a href="/resources/protocols/adipogenic-differentiation-humanmouse-mesenchymal-stem-cells" rel="tag" title="Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells">Read more<span class="element-invisible"> about Adipogenic Differentiation of Human/Mouse Mesenchymal Stem Cells</span></a></li> </ul> </div> <div id="node-2816" class="node node-protocols node-teaser clearfix" about="/resources/protocols/procedure-expansion-human-mesenchymal-stem-cells" typeof="sioc:Item foaf:Document"> <h2><a href="/resources/protocols/procedure-expansion-human-mesenchymal-stem-cells">Procedure for the Expansion of Human Mesenchymal Stem Cells</a></h2> <span property="dc:title" content="Procedure for the Expansion of Human Mesenchymal Stem Cells" class="rdf-meta element-hidden"></span><span property="sioc:num_replies" content="0" datatype="xsd:integer" class="rdf-meta element-hidden"></span> <div class="content"> <div class="field field-name-body field-type-text-with-summary field-label-hidden"><div class="field-items"><div class="field-item even" property="content:encoded"><p>Mesenchymal stem cells (MSCs) are functionally defined by their capacity to self renew and their ability to differentiate into multiple cell types including adipocytes, chondrocytes, and osteocytes (1, 2). MSCs are phenotypically characterized as CD34<sup>-</sup>, CD105<sup>+</sup>, and CD90<sup>+</sup> cells. This protocol describes the expansion of human MSCs using the <a href="/products/stemxvivo-mesenchymal-stem-cell-expansion-media_ccm004">StemXVivo™ MSC Expansion Media</a> (R&D Systems, Catalog # CCM004).</p> <p>Please read the protocol in its entirety before starting.</p></div></div></div> </div> <ul class="links inline"><li class="node-readmore first last"><a href="/resources/protocols/procedure-expansion-human-mesenchymal-stem-cells" rel="tag" title="Procedure for the Expansion of Human Mesenchymal Stem Cells">Read more<span class="element-invisible"> about Procedure for the Expansion of Human Mesenchymal Stem Cells</span></a></li> </ul> </div> <h2 class="element-invisible">Pages</h2><div class="item-list"><ul class="pager"><li class="pager-current first">1</li> <li class="pager-item"><a title="Go to page 2" href="/protocol-types/stem-cell-protocols?page=1">2</a></li> <li class="pager-next"><a title="Go to next page" href="/protocol-types/stem-cell-protocols?page=1">next ›</a></li> 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