CINXE.COM

<!doctype html> <html lang="ta"> <head> <title>A Simple Dilute-and-Shoot Flow-Injection Mass Spectrometric | 25512</title> <meta name="keywords" content="Raghavi Kakarla, Ramakrishna Reddy Voggu, Janet Donaldson and Baochuan Guo , "/> <meta name="description" content="Levels of glycocholic acid are elevated in the bile of cholangiocarcinoma patients, making it a potential biomarker for the disease. The utility of liquid ..25512"/> <meta name="citation_publisher" content="லாங்டம் பப்ளிஷிங் எஸ்.எல்" /> <meta name="citation_journal_title" content="மாஸ் ஸ்பெக்ட்ரோமெட்ரி & சுத்திகரிப்பு நுட்பங்கள்"> <meta name="citation_title" content="A Simple Dilute-and-Shoot Flow-Injection Mass Spectrometric Method for Quantification of Glycocholic Acid in Human Bile"> <meta name="citation_author" content="Raghavi Kakarla" /> <meta name="citation_author" content="Ramakrishna Reddy Voggu" /> <meta name="citation_author" content="Janet Donaldson" /> <meta name="citation_author" content="Baochuan Guo" /> <meta name="citation_year" content=""> <meta name="citation_volume" content="5"> <meta name="citation_issue" content="1"> <meta name="citation_abstract" content="Levels of glycocholic acid are elevated in the bile of cholangiocarcinoma patients, making it a potential biomarker for the disease. The utility of liquid chromatography mass spectrometry based methods for the quantification of glycocholic acid often suffers from time-consuming extraction steps, carryover, and unavailability of blank bile matrix. To overcome the problems, we developed a dilute-and-shoot flow-injection tandem mass spectrometry (standard addition-based) method for quantification of glycocholic acid in bile. Bile was first diluted, followed by spiking the glycocholic acid standard and internal standard into the diluted bile, which was then directly flow-injected (without chromatographic separation) into the electrospray ionization source. Detection was carried out in negative multiple reaction monitoring mode with m/z transitions set at 464.1→74.0 and 401.2→249.1 for glycocholic acid and internal standard, respectively. A standard addition calibration curve was constructed to determine the amount of glycocholic acid present in human bile. The method has been validated with clinical samples according to FDA guidelines. Linearity was achieved in the spiked concentration range of 12.5 to 200 ng/mL with a correlation coefficient of 0.9857. The method has good accuracy, precision, minimal matrix effect, and is sufficiently sensitive enough to quantitate glycocholic acid even after 800,000-fold dilution. In conclusion, a dilute and shoot flow-injection MS/MS method has been developed and validated for the first time, which could be used in routine analysis of glycocholic acid in human bile in a clinical setting."> <meta name="citation_abstract_html_url" content="https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html"> <meta name="format-detection" content="telephone=no"> <meta http-equiv="Content-Language" content="ta"> <meta name="google-site-verification" content="NomPTP94YozsgvD3NEFpNqUfY88e0TU0L64zNzZTpd0" /> <meta itemprop="name" content="longdom" /> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="ROBOTS" content="INDEX,FOLLOW"> <meta name="googlebot" content="INDEX,FOLLOW"> <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no"> <link rel="canonical" href="https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html"> <link rel="alternate" href="https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" hreflang="ta-in" />  <link rel="stylesheet" href="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/css/bootstrap.min.css"> <link href="/assets/css/longdom.css" rel="stylesheet"> <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/animate.css/3.7.0/animate.min.css">  <link rel="stylesheet" href="https://use.fontawesome.com/releases/v5.7.1/css/all.css"> <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.3.0/css/font-awesome.min.css">  <link href="https://fonts.googleapis.com/css?family=Montserrat:100,100i,200,200i,300,300i,400,400i,500,500i,600,600i,700,700i,800,800i,900,900i|Open+Sans:300,300i,400,400i,600,600i,700,700i,800,800i|Raleway:100,100i,200,200i,300,300i,400,400i,500,500i,600,600i,700,700i,800,800i,900,900i" rel="stylesheet"> <link href="/assets/css/ionicons.min.css" rel="stylesheet">  <link rel="stylesheet" href="/assets/css/jquery.mCustomScrollbar.min.css"> <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/bootstrap-select/1.13.2/css/bootstrap-select.min.css">  <link rel="stylesheet" href="/assets/css/global.css">  <link rel="stylesheet" href="/assets/css/styles.css"> <link rel="stylesheet" type="text/css" href="/assets/css/author.css"> <link rel="icon" href="/assets/img/favicon.png" type="image/gif"> <link rel="stylesheet" href="/assets/css/coolautosuggest.css">  <script async src="https://www.googletagmanager.com/gtag/js?id=G-GH5GSYYKJG"></script> <script async src="https://pagead2.googlesyndication.com/pagead/js/adsbygoogle.js?client=ca-pub-8046643215361735" crossorigin="anonymous"></script> <script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-PEN3HZECLN'); </script> </head> <body> <header>  <nav class="navbar navbar-expand-lg navbar-light bg-white shadow-sm deva541"> <div class="container"> <a class="navbar-brand" href="https://tamil.longdom.org/" title="இங்கே கிளிக் செய்யவும்"> <img src="/assets/img/longdom-logo.svg" alt="Logo" height="25"> </a> <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#navbar" aria-controls="navbar" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="collapse navbar-collapse justify-content-end" id="navbar"> <div class="navbar-nav"> <a class="nav-item nav-link" href="https://tamil.longdom.org/" title="இங்கே கிளிக் செய்யவும்">வீடு</a> <a class="nav-item nav-link" href="https://tamil.longdom.org/open-access-journals-list.html" title="இங்கே கிளிக் செய்யவும்">இதழ்கள்</a> <div class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="Guidelines" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">வழிகாட்டுதல்கள் & கொள்கைகள்</a> <div class="dropdown-menu" aria-labelledby="இங்கே கிளிக் செய்யவும்"> <a class="dropdown-item" href="https://tamil.longdom.org/editorial-policies.html" title="இங்கே கிளிக் செய்யவும்">தலையங்கக் கொள்கைகள்</a> <a class="dropdown-item" href="https://tamil.longdom.org/instructions-to-authors.html" title="இங்கே கிளிக் செய்யவும்">ஆசிரியர்களுக்கான வழிமுறைகள் </a> <a class="dropdown-item" href="https://tamil.longdom.org/policies.html" title="இங்கே கிளிக் செய்யவும்">கொள்கைகள்</a> <a class="dropdown-item" href="https://tamil.longdom.org/publication-ethics.html" title="இங்கே கிளிக் செய்யவும்">வெளியீட்டு நெறிமுறைகள்</a> <a class="dropdown-item" href="https://tamil.longdom.org/reviewers.html" title="இங்கே கிளிக் செய்யவும்">விமர்சகர்கள்</a> <a class="dropdown-item" href="https://tamil.longdom.org/terms-conditions.html" title="இங்கே கிளிக் செய்யவும்">விதிமுறைகள் மற்றும் நிபந்தனைகள்</a> </div> </div> <a class="nav-item nav-link" href="https://tamil.longdom.org/advertising.html" title="இங்கே கிளிக் செய்யவும்">விளம்பரம்</a> <a class="nav-item nav-link" href="https://tamil.longdom.org/conferences.html" title="இங்கே கிளிக் செய்யவும்">மாநாடுகள்</a> <a class="nav-item nav-link" href="https://tamil.longdom.org/contact-us.html" title="இங்கே கிளிக் செய்யவும்">எங்களை தொடர்பு கொள்ள</a>  <div class="nav-item dropdown multi_lang j_multi_lang"> <a class="nav-link dropdown-toggle" href="#" id="multi_lang" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">Language</a> <div class="dropdown-menu" aria-labelledby=""> <a class="dropdown-item" href="https://www.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" title="English"> <img src="https://www.longdom.org/admin/flags/usa.png">English </a> <a class="dropdown-item" href="https://spanish.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" title="Spanish"> <img src="https://www.longdom.org/admin/flags/spain.png">Spanish </a> <a class="dropdown-item" href="https://chinese.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" title="Chinese"> <img src="https://www.longdom.org/admin/flags/china.png">Chinese </a> <a class="dropdown-item" href="https://russian.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" title="Russian"> <img src="https://www.longdom.org/admin/flags/russia.png">Russian </a> <a class="dropdown-item" href="https://german.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" title="German"> <img src="https://www.longdom.org/admin/flags/germany.png">German </a> <a class="dropdown-item" href="https://french.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" title="French"> <img src="https://www.longdom.org/admin/flags/france.png">French </a> <a class="dropdown-item" href="https://japanese.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" title="Japanese"> <img src="https://www.longdom.org/admin/flags/japan.png">Japanese </a> <a class="dropdown-item" href="https://portuguese.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" title="Portuguese"> <img src="https://www.longdom.org/admin/flags/portugal.png">Portuguese </a> <a class="dropdown-item" href="https://hindi.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" title="Hindi"> <img src="https://www.longdom.org/admin/flags/india.png">Hindi </a> <a class="dropdown-item" href="https://telugu.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" title="Telugu"> <img src="https://www.longdom.org/admin/flags/india.png">Telugu </a> </div> </div>  </div>  </div> </div> </nav> </header>  <section class="bg-info py-1"> <div class="container"> <div class="row align-items-center justify-content-between">  <div class="col-12 col-sm-auto"> <img src="https://www.longdom.org/admin/headers/mass-spectrometry--purification-techniques-logo.svg" alt="மாஸ் ஸ்பெக்ட்ரோமெட்ரி & சுத்திகரிப்பு நுட்பங்கள்" width="105" height="105" class="img-fluid mx-auto"> </div> <div class="col-12 col-sm-8"> <h1 class="text-left text-white border-light-blue-200-before font-size-7">மாஸ் ஸ்பெக்ட்ரோமெட்ரி & சுத்திகரிப்பு நுட்பங்கள்<br><small class="float-right font-size-5">திறந்த அணுகல்</small></h1> </div>  <div class="col-12 col-sm-2 d-none d-sm-block"> <p class="lead">ஐ.எஸ்.எஸ்.என்: 2469-9861</p>  </div> </div> </div> </section>  <nav id="sticky-navbar" class="navbar navbar-expand-lg navbar-dark bg-primary py-0"> <div class="container"> <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#jrnlNavbar" aria-controls="jrnlNavbar" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="collapse navbar-collapse justify-content-center" id="jrnlNavbar"> <div class="navbar-nav"> <a class="nav-item nav-link " href="https://tamil.longdom.org/mass-spectrometry-purification-techniques.html" title="இங்கே கிளிக் செய்யவும்">ஜர்னல் ஹோம்</a> <a class="nav-link " href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/aim-and-scope.html" title="இங்கே கிளிக் செய்யவும்">நோக்கம் மற்றும் நோக்கம்</a> <div class="nav-item dropdown"> <a class="nav-link dropdown-toggle " title="இங்கே கிளிக் செய்யவும்" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false" href="#">ஆசிரியர்களுக்கான வழிமுறைகள் <i class="fas fa-caret-down"></i></a> <div class="dropdown-menu" aria-labelledby="EditorialPanel"> <a class="dropdown-item" href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/instructionsforauthors.html" title="இங்கே கிளிக் செய்யவும்">ஆசிரியர்களுக்கான வழிமுறைகள்</a> <a class="dropdown-item" href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/ethical-malpractices.html" title="இங்கே கிளிக் செய்யவும்">வெளியீட்டு நெறிமுறைகள் மற்றும் முறைகேடு அறிக்கை</a> </div> </div> <a class="nav-link " href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/submit-manuscript.html" title="இங்கே கிளிக் செய்யவும்">கையெழுத்துப் பிரதியை சமர்ப்பிக்கவும்</a> <a class="nav-link " href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/archive.html" title="இங்கே கிளிக் செய்யவும்">காப்பகம்</a> <a class="nav-link " href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/guidelines.html" title="இங்கே கிளிக் செய்யவும்">வழிகாட்டுதல்கள்</a> <a class="nav-item nav-link " href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/contact.html" title="இங்கே கிளிக் செய்யவும்">தொடர்பு கொள்ளவும்</a> </div> </div> </div> </nav> <section class="py-4 content"> <div class="container"> <div class="row"> <aside class="col-12 col-sm-3 order-last order-sm-first">  <div class="card shadow-sm sidebar mb-3" style="margin-top:10px"> <h6 class="card-header">இந்தக் கட்டுரையைப் பகிரவும்</h6> <div class="card-body"> <nav class="nav social-icons social-icons-sm"> <a class="nav-link bg-facebook white" href="https://www.facebook.com/sharer.php?u=https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" target="_blank" title="Share with Facebook" rel="noopener"><i class="fab fa-facebook-f"></i></a> <a class="nav-link bg-twitter white" href="https://twitter.com/share?url=https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" rel="noopener" target="_blank" title="Share with Twitter"><i class="fab fa-twitter"></i></a> <a class="nav-link bg-linkedin white" href="https://www.linkedin.com/shareArticle?mini=true&url=https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" rel="noopener" target="_blank" title="Share with Linkdin"><i class="fab fa-linkedin-in"></i></a> <a class="nav-link bg-googleplus white" href="https://plus.google.com/share?url=https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" target="_blank" rel="noopener" title="Share with Google+"><i class="fab fa-google-plus-g"></i></a> <a class="nav-link bg-pinterest white" href="https://pinterest.com/pin/create/button/?url=https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" rel="noopener" target="_blank" title="Share with Pintrest"><i class="fab fa-pinterest-p"></i></a> <a class="nav-link bg-blogger white" href="https://www.blogger.com/blog-this.g?u=https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" rel="noopener" target="_blank" title="Share with Blogger"><i class="fab fa-blogger-b"></i></a> </nav> </div> </div> <div class="card shadow-sm sidebar mb-3"> <h6 class="card-header pr-0">குறியிடப்பட்டது</h6> <div class="list-group list-group-flush overflow-view"> <div class="list-group-item p-0 pt-2 pl-2"> <img src="https://www.longdom.org//admin/indexing-logos/refseek-49.jpg" alt="Logo" width="50%"> </div> <div class="list-group-item p-0 pt-2 pl-2"> <img src="https://www.longdom.org//admin/indexing-logos/directory-of-research-journal-indexing-drji-51.jpg" alt="Logo" width="50%"> </div> <div class="list-group-item p-0 pt-2 pl-2"> <img src="https://www.longdom.org//admin/indexing-logos/hamdard-university-57.jpg" alt="Logo" width="50%"> </div> <div class="list-group-item p-0 pt-2 pl-2"> <img src="https://www.longdom.org//admin/indexing-logos/ebsco-az-58.jpg" alt="Logo" width="50%"> </div> <div class="list-group-item p-0 pt-2 pl-2"> <img src="https://www.longdom.org//admin/indexing-logos/oclc-worldcat-75.jpg" alt="Logo" width="50%"> </div> <div class="list-group-item p-0 pt-2 pl-2"> <img src="https://www.longdom.org//admin/indexing-logos/scholarsteer-77.jpg" alt="Logo" width="50%"> </div> <div class="list-group-item p-0 pt-2 pl-2"> <img src="https://www.longdom.org//admin/indexing-logos/publons-84.png" alt="Logo" width="50%"> </div> <div class="list-group-item p-0 pt-2 pl-2"> <img src="https://www.longdom.org//admin/indexing-logos/euro-pub-123.png" alt="Logo" width="50%"> </div> <div class="list-group-item p-0 pt-2 pl-2"> <img src="https://www.longdom.org//admin/indexing-logos/google-scholar-124.jpg" alt="Logo" width="50%"> </div> </div> <p class="m-0 clearfix"><a href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/indexing.html" title="இங்கே கிளிக் செய்யவும்"><span class="btn btn-warning btn-xs float-right">மேலும் பார்க்க »</span></a></p> </div>   <div class="card shadow-sm sidebar mb-3"> <h6 class="card-header">பயனுள்ள இணைப்புகள்</h6> <div class="card-body p-0"> <nav class="nav flex-column font-size-3 icon-list icon-list-angle-right a-py-1"> <a class="nav-item nav-link" href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/aim-and-scope.html" title="இங்கே கிளிக் செய்யவும்">நோக்கம் மற்றும் நோக்கம்</a> <a class="nav-item nav-link" href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/peer-review-process.html" title="இங்கே கிளிக் செய்யவும்">சக மதிப்பாய்வு செயல்முறை</a> <a class="nav-item nav-link" href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/indexing.html" title="இங்கே கிளிக் செய்யவும்">அட்டவணைப்படுத்துதல் மற்றும் காப்பகப்படுத்துதல்</a> <a class="nav-item nav-link" href="https://tamil.longdom.org/mass-spectrometry-purification-techniques/archive.html" title="இங்கே கிளிக் செய்யவும்">அட்டவணைப்படுத்துதல் மற்றும் காப்பகப்படுத்துதல்</a> <a class="nav-item nav-link" href="https://www.longdom.org/submissions/mass-spectrometry-purification-techniques.html" title="இங்கே கிளிக் செய்யவும்">கையெழுத்துப் பிரதியை சமர்ப்பிக்கவும்</a> </nav> </div> </div>     <div class="card shadow-sm sidebar mb-3"> <h6 class="card-header">அணுகல் இதழ்களைத் திறக்கவும்</h6> <div class="card-body p-0 scroll_mang"> <nav class="nav flex-column font-size-3 icon-list icon-list-angle-right a-py-1"> <a class="nav-item nav-link" href="https://tamil.longdom.org/food-and-nutrition-journals.html" title="இங்கே கிளிக் செய்யவும்">உணவு மற்றும் ஊட்டச்சத்து</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/biochemistry-journals.html" title="இங்கே கிளிக் செய்யவும்">உயிர்வேதியியல்</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/neuroscience-and-psychology-journals.html" title="இங்கே கிளிக் செய்யவும்">நரம்பியல் & உளவியல்</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/nursing-and-health-care-journals.html" title="இங்கே கிளிக் செய்யவும்">நர்சிங் & ஹெல்த் கேர்</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/immunology-and-microbiology-journals.html" title="இங்கே கிளிக் செய்யவும்">நோயெதிர்ப்பு மற்றும் நுண்ணுயிரியல்</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/bioinformatics-and-systems-biology-journals.html" title="இங்கே கிளிக் செய்யவும்">பயோ இன்ஃபர்மேடிக்ஸ் & சிஸ்டம்ஸ் பயாலஜி</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/general-science-journals.html" title="இங்கே கிளிக் செய்யவும்">பொது அறிவியல்</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/engineering-journals.html" title="இங்கே கிளிக் செய்யவும்">பொறியியல்</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/genetics-and-molecular-biology-journals.html" title="இங்கே கிளிக் செய்யவும்">மரபியல் & மூலக்கூறு உயிரியல்</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/clinical-sciences-journals.html" title="இங்கே கிளிக் செய்யவும்">மருத்துவ அறிவியல்</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/medical-sciences-journals.html" title="இங்கே கிளிக் செய்யவும்">மருத்துவ அறிவியல்</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/pharmaceutical-sciences-journals.html" title="இங்கே கிளிக் செய்யவும்">மருந்து அறிவியல்</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/business-and-management-journals.html" title="இங்கே கிளிக் செய்யவும்">வணிக மேலாண்மை</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/agri-and-aquaculture-journals.html" title="இங்கே கிளிக் செய்யவும்">விவசாயம் மற்றும் மீன் வளர்ப்பு</a><br> <a class="nav-item nav-link" href="https://tamil.longdom.org/chemistry-journals.html" title="இங்கே கிளிக் செய்யவும்">வேதியியல்</a><br> </nav> </div> </div>  </aside> <div class="col-12 col-sm-9"> <h2 class="heading-1">சுருக்கம்</h2> <h3 class="">A Simple Dilute-and-Shoot Flow-Injection Mass Spectrometric Method for Quantification of Glycocholic Acid in Human Bile</h3> <p> <i class="fas fa-user"></i> Raghavi Kakarla, Ramakrishna Reddy Voggu, Janet Donaldson and Baochuan Guo </p> Levels of glycocholic acid are elevated in the bile of cholangiocarcinoma patients, making it a potential biomarker for the disease. The utility of liquid chromatography mass spectrometry based methods for the quantification of glycocholic acid often suffers from time-consuming extraction steps, carryover, and unavailability of blank bile matrix. To overcome the problems, we developed a dilute-and-shoot flow-injection tandem mass spectrometry (standard addition-based) method for quantification of glycocholic acid in bile. Bile was first diluted, followed by spiking the glycocholic acid standard and internal standard into the diluted bile, which was then directly flow-injected (without chromatographic separation) into the electrospray ionization source. Detection was carried out in negative multiple reaction monitoring mode with m/z transitions set at 464.1→74.0 and 401.2→249.1 for glycocholic acid and internal standard, respectively. A standard addition calibration curve was constructed to determine the amount of glycocholic acid present in human bile. The method has been validated with clinical samples according to FDA guidelines. Linearity was achieved in the spiked concentration range of 12.5 to 200 ng/mL with a correlation coefficient of 0.9857. The method has good accuracy, precision, minimal matrix effect, and is sufficiently sensitive enough to quantitate glycocholic acid even after 800,000-fold dilution. In conclusion, a dilute and shoot flow-injection MS/MS method has been developed and validated for the first time, which could be used in routine analysis of glycocholic acid in human bile in a clinical setting. <div class="alert alert-info text-left"><b>மறுப்பு:</b> இந்த சுருக்கமானது செயற்கை நுண்ணறிவு கருவிகளைப் பயன்படுத்தி மொழிபெயர்க்கப்பட்டது மற்றும் இன்னும் மதிப்பாய்வு செய்யப்படவில்லை அல்லது சரிபார்க்கப்படவில்லை.</div> <div class="nav social-icons"> <a class="nav-link w-auto">இந்தக் கட்டுரையைப் பகிரவும்</a> <a title="இங்கே கிளிக் செய்யவும்" target="_blank" href="https://www.facebook.com/sharer.php?u=https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" rel="noopener"><i class="fab fa-facebook-f"></i></a> <a class="nav-link" title="இங்கே கிளிக் செய்யவும்" target="_blank" href="https://twitter.com/share?url=https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" rel="noopener"><i class="fab fa-twitter"></i></a> <a class="nav-link" title="இங்கே கிளிக் செய்யவும்" target="_blank" href="https://www.linkedin.com/shareArticle?mini=true&url=https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" rel="noopener"><i class="fab fa-linkedin-in"></i></a> <a class="nav-link" title="இங்கே கிளிக் செய்யவும்" target="_blank" href="https://plus.google.com/share?url=https://tamil.longdom.org/abstract/a-simple-diluteandshoot-flowinjection-mass-spectrometric-method-for-quantification-of-glycocholic-acid-in-human-bile-25512.html" rel="noopener"><i class="fab fa-google-plus-g"></i></a> </div> </div> </div> </div> </section> <footer class="bg-blue-grey-900 py-3"> <div class="container"> <div class="row"> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">உள்ளடக்க இணைப்புகள்</h4> <ul class="list-unstyled footer-links font-size-3"> <li><a class="" href="https://tamil.longdom.org/privacy-policy.html" title="இங்கே கிளிக் செய்யவும்">தனியுரிமைக் கொள்கை</a></li> <li><a class="" href="https://tamil.longdom.org/terms-conditions.html" title="இங்கே கிளிக் செய்யவும்">விதிமுறைகளும் நிபந்தனைகளும்</a></li> <li><a class="" href="https://tamil.longdom.org/authors-reviewers-editors.html" title="இங்கே கிளிக் செய்யவும்">ஆசிரியர்கள், விமர்சகர்கள் & எடிட்டோ </a></li> </ul> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">லாங்டம் தொடர்பு கொள்ளவும்</h4> <p><strong>லாங்டம் குழு SA</strong><br /> Avenue Roger Vandendriessche,<br /> 18, 1150 பிரஸ்ஸல்ஸ், பெல்ஜியம்<br /> தொலைபேசி: +442038085340<br /> <strong>மின்னஞ்சல்:</strong> <a class="white" href="mailto:info@longdom.org" title="Click here">info@longdom.org</a></p> </div> <div class="col-12 col-sm-4"> <h4 class="white font-size-4 fweight-400 border-bottom-1 pb-2">இணைக்கவும்</h4> <nav class="nav nav-pills social-icons-footer flex-column a-pl-0"> <a href="https://www.facebook.com/longdompublisher" title="இங்கே கிளிக் செய்யவும்" target="_blank" class="nav-link bg-facebook-hover"><i class="fab fa-facebook-f bg-facebook"></i></a> <a href="https://www.linkedin.com/company/longdom-publishing-sl/" title="இங்கே கிளிக் செய்யவும்" target="_blank" class="nav-link bg-linkedin-hover"><i class="fab fa-linkedin-in bg-linkedin"></i></a> <a href="https://twitter.com/LongdomP" title="இங்கே கிளிக் செய்யவும்" target="_blank" class="nav-link bg-twitter-hover"><i class="fab fa-twitter bg-twitter"></i></a> <a href="https://www.instagram.com/longdom_publisher/" title="இங்கே கிளிக் செய்யவும்" target="_blank" class="nav-link bg-instagram-hover"><i class="fab fa-instagram bg-instagram"></i></a> </nav> </div> </div> <div class="row text-center"> <div class="col"> <p>காப்புரிமை © 2024 <a href="https://tamil.longdom.org/" title="இங்கே கிளிக் செய்யவும்" class="white">லாங்டம் பப்ளிஷிங் எஸ்.எல்</a>.</p> </div> </div> </div> </footer>  <a href="#0" class="cd-top js-cd-top">Top</a>   <script src="https://code.jquery.com/jquery-3.3.1.min.js"></script> <script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.14.7/umd/popper.min.js"></script> <script src="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/js/bootstrap.min.js"></script>  <script> jQuery(function($) { $(window).scroll(function fix_element() { $('#target').css( $(window).scrollTop() > 100 ? { 'position': 'fixed', 'top': '440px' } : { 'position': 'absolute', 'top': '440px' } ); return fix_element; }()); }); </script>   <script src="https://unpkg.com/feather-icons"></script> <script> feather.replace() </script>  <script src="/assets/js/scroll-to-top.js"></script>  <script type="text/javascript" src="/assets/js/coolautosuggest.js"></script> <script language="javascript" type="text/javascript"> $("#keyword").coolautosuggest({ url: "https://tamil.longdom.org/author-names.php?chars=", minChars: 3, }); </script> <script src="/assets/js/jquery.mCustomScrollbar.concat.min.js"></script> <script> // Scrollbar var Scrollbar = function() { "use strict"; // Handle Scrollbar Linear var handleScrollbarLinear = function() { $(".scrollbar").mCustomScrollbar({ theme: "minimal-dark" }); } return { init: function() { handleScrollbarLinear(); // initial setup for scrollbar linear } } }(); $(document).ready(function() { Scrollbar.init(); }); /*========================== Stikcy Navbar ============================*/ window.onscroll = function() { myFunction() }; var navbar = document.getElementById("sticky-navbar"); var sticky = navbar.offsetTop; function myFunction() { if (window.pageYOffset >= sticky) { navbar.classList.add("sticky") } else { navbar.classList.remove("sticky"); } } /*========================== Bootstrap Popover ============================*/ $(function () { $('[data-toggle="popover"]').popover() }) </script>  <script src="/assets/js/jquery.malihu.PageScroll2id.min.js"></script> <script> (function($){ $(window).on("load",function(){ $("a[rel='m_PageScroll2id']").mPageScroll2id(); }); })(jQuery); </script>  <script type="text/javascript" src="/assets/js/jquery.matchHeight-min.js"></script> <script> $(function() { $('.match-height').matchHeight({ byRow: true, property: 'height', target: null, }); }); </script> <script type="text/javascript" src="/assets/js/grids.min.js"></script> <script type="text/javascript"> // Equal Height var EqualHeight = function() { "use strict"; // Handle Equal Height var handleEqualHeight = function() { $(function($) { $('.equal-height').responsiveEqualHeightGrid(); }); } return { init: function() { handleEqualHeight(); // initial setup for equal height } } }(); $(document).ready(function() { EqualHeight.init(); }); </script>  <script src="https://cdnjs.cloudflare.com/ajax/libs/bootstrap-select/1.13.2/js/bootstrap-select.min.js"></script> <script> $('.selectpicker').selectpicker(); </script> <script> $(document).ready(function(){ var filecount = 1; $('.filerowclass').each(function(){ var countattr = $(this).attr('countattr'); if(filecount == countattr){ var countlink = $('#rowfile'+countattr+ ' .filelinkclass').length; if(countlink == 0){ $('#rowfile'+countattr).remove(); } } filecount++; }); }); </script> <script type="text/javascript">function add_chatinline(){var hccid=93028499;var nt=document.createElement("script");nt.async=true;nt.src="https://mylivechat.com/chatinline.aspx?hccid="+hccid;var ct=document.getElementsByTagName("script")[0];ct.parentNode.insertBefore(nt,ct);} add_chatinline(); </script><link href="https://cdn.jsdelivr.net/npm/select2@4.1.0-rc.0/dist/css/select2.min.css" rel="stylesheet" /> <script src="https://cdn.jsdelivr.net/npm/select2@4.1.0-rc.0/dist/js/select2.min.js"></script> </body> </html>