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Lipases with this ability have been reported in the yeast Candida rugosa that secretes several extracellular enzymes with a high level of sequence identity, although different substrate specificity. This versatility has also been found in the sterol esterases from the ascomycetes Ophiostoma piceae and Melanocarpus albomyces.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147456" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="472d1ed533abed391f3eab203d94bab0" rel="nofollow" data-download="{"attachment_id":44499926,"asset_id":24147456,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44499926/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24147456 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24147456"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24147456, container: ".js-paper-rank-work_24147456", }); 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Methods and Results: After an inoculation assay, except for two, all strains... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147472" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Aims: In this study, 10 putative plant growth-promoting rhizobacteria (PGPR) were assayed for their ability to improve Pinus pinea growth and mycorrhization. Methods and Results: After an inoculation assay, except for two, all strains stimulated plant growth. All bacteria altered rhizosphere microbial communities as revealed by phospholipid fatty acid analysis; associating plant growth promotion with a decrease in biological diversity. Three strains were tested for their ability to enhance pine mycorrhization with wild fungi species. Only strain BB1 increased the total number of mycorrhizal root tips. Mycorrhizas present in the roots of each treatment were identified by ribosomal RNA sequencing and denaturing gradient gel electrophoresis analysis, detecting specificity between mycorrhizal species colonizing the roots and the inoculated PGPR. Conclusions: In conclusion, BB1 appears to be a good candidate to be developed into a biofertilizer directed to enhance pine growth and mycorrhization, which should result in a better establishment rate for plants used in reforestation. Significance and Impact of the Study: This study shows the potential of PGPR to improve fitness of forest tree specie. Moreover, the specificity between the bacteria inoculated and the mycorrhiza that the plant selects involve a potential biotechnological use in production of value-added fungi.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147472" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="560638d94cb83ae45303d929a2d1599b" rel="nofollow" data-download="{"attachment_id":44499934,"asset_id":24147472,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44499934/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-24147472">+1</span><div class="hidden js-additional-users-24147472"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/GutierrezManero">Gutiérrez Mañero</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-24147472'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-24147472').html(); 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Methods and Results: After an inoculation assay, except for two, all strains stimulated plant growth. All bacteria altered rhizosphere microbial communities as revealed by phospholipid fatty acid analysis; associating plant growth promotion with a decrease in biological diversity. Three strains were tested for their ability to enhance pine mycorrhization with wild fungi species. Only strain BB1 increased the total number of mycorrhizal root tips. Mycorrhizas present in the roots of each treatment were identified by ribosomal RNA sequencing and denaturing gradient gel electrophoresis analysis, detecting specificity between mycorrhizal species colonizing the roots and the inoculated PGPR. Conclusions: In conclusion, BB1 appears to be a good candidate to be developed into a biofertilizer directed to enhance pine growth and mycorrhization, which should result in a better establishment rate for plants used in reforestation. Significance and Impact of the Study: This study shows the potential of PGPR to improve fitness of forest tree specie. Moreover, the specificity between the bacteria inoculated and the mycorrhiza that the plant selects involve a potential biotechnological use in production of value-added fungi.","publication":"Journal of Applied Microbiology","publication_with_fallback":"Journal of Applied 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})();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24147476 coauthored" data-work_id="24147476" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24147476/Screening_for_Putative_PGPR_to_Improve_Establishment_of_the_Symbiosis_Lactarius_deliciosus_Pinus_sp">Screening for Putative PGPR to Improve Establishment of the Symbiosis Lactarius deliciosus-Pinus sp</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">A screening for plant growth promoting rhizobacteria (PGPR) was carried out in the mycorrhizosphere of wild populations of Pinus pinea and P. pinaster, and in the mycosphere of associated Lactarius deliciosus. A total of 720 bacteria were... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147476" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">A screening for plant growth promoting rhizobacteria (PGPR) was carried out in the mycorrhizosphere of wild populations of Pinus pinea and P. pinaster, and in the mycosphere of associated Lactarius deliciosus. A total of 720 bacteria were isolated, purified, and grouped by morphological criteria. Fifty percent of the isolates were selected and tested for aminocyclopropanecarboxylic acid (ACC) degradation, auxin and siderophore production, and phosphate solubilization. Thirty eight percent of the isolates showed at least one of the evaluated activities. Nutrient-related traits were associated with P. pinaster, whereas hormone production traits predominated in P. pinea. These activities were found mostly in Gram positive isolates. After PCR-RAPDs (random amplified polymorphic DNA) analysis, 10 groups appeared with 85% similiarity when considering all isolates, indicating the low diversity in the system. One strain of each group was identified by 16S rDNA sequencing. Our results suggest that P. pinaster selects for mycorrhizosphere bacteria that mobilize nutrients, whereas P. pinea selects for bacteria that have the capacity to increase root growth via production of plant growth regulators.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147476" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="cb60a4e78cc838e4691745da533a3a66" rel="nofollow" data-download="{"attachment_id":44499930,"asset_id":24147476,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44499930/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-24147476">+1</span><div class="hidden js-additional-users-24147476"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/GutierrezManero">Gutiérrez Mañero</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-24147476'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-24147476').html(); 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u-tcGrayDarkest"><div class="summarized">. Protection against pathogen and salt stress by four plant growth-promoting rhizobacteria isolated from Pinus sp. on Arabidopsis thaliana. Phytopathology 98:666-672. The ability of four plant growth-promoting rhizobacteria, isolated in a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147480" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">. Protection against pathogen and salt stress by four plant growth-promoting rhizobacteria isolated from Pinus sp. on Arabidopsis thaliana. Phytopathology 98:666-672. The ability of four plant growth-promoting rhizobacteria, isolated in a previous study, to induce systemic resistance on Arabidopsis thaliana Col 0 against biotic and abiotic stress was evaluated. All the bacteria enhanced protection against the foliar pathogen Pseudomonas syringae DC3000 and increased plant tolerance to salt stress (NaCl 60 mM). Bacillus sp. strain L81 and Arthrobacter oxidans strain BB1 performed best with a decrease in the disease index of 61.2 and 52.3%, respectively, and a reduction in the mortality due to salt stress of 72.4 and 57.8%, respectively. Additionally, significant differences were found in growth and photosynthesis, again, L81 and BB1 performed best either in normal or under stress conditions. In order to elucidate the pathway elicited by these two strains to induce systemic resistance, experiments with the transgenic line of Arabidopsis thaliana NahG (defective in salicylic acid [SA]) and with the jar1 mutant (defective in jasmonic acid) were carried out. Results showed that the SA-dependent pathway was involved in the defense response induced by strains L81 and BB1. Results from quantitative reverse transcription-polymerase chain reaction analysis of the PR1 gene, related to the SA-dependent pathway and the PDF1.2 gene related to the SAindependent pathway, showed an increased expression of PR1 in BB1treated plants, confirming involvement of the SA-dependent pathway in the defensive response.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147480" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="5dd07999fb397345e64c291fee2ca965" rel="nofollow" data-download="{"attachment_id":44499938,"asset_id":24147480,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44499938/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-24147480">+1</span><div class="hidden js-additional-users-24147480"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/GutierrezManero">Gutiérrez Mañero</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-24147480'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-24147480').html(); 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Protection against pathogen and salt stress by four plant growth-promoting rhizobacteria isolated from Pinus sp. on Arabidopsis thaliana. Phytopathology 98:666-672. The ability of four plant growth-promoting rhizobacteria, isolated in a previous study, to induce systemic resistance on Arabidopsis thaliana Col 0 against biotic and abiotic stress was evaluated. All the bacteria enhanced protection against the foliar pathogen Pseudomonas syringae DC3000 and increased plant tolerance to salt stress (NaCl 60 mM). Bacillus sp. strain L81 and Arthrobacter oxidans strain BB1 performed best with a decrease in the disease index of 61.2 and 52.3%, respectively, and a reduction in the mortality due to salt stress of 72.4 and 57.8%, respectively. Additionally, significant differences were found in growth and photosynthesis, again, L81 and BB1 performed best either in normal or under stress conditions. In order to elucidate the pathway elicited by these two strains to induce systemic resistance, experiments with the transgenic line of Arabidopsis thaliana NahG (defective in salicylic acid [SA]) and with the jar1 mutant (defective in jasmonic acid) were carried out. Results showed that the SA-dependent pathway was involved in the defense response induced by strains L81 and BB1. Results from quantitative reverse transcription-polymerase chain reaction analysis of the PR1 gene, related to the SA-dependent pathway and the PDF1.2 gene related to the SAindependent pathway, showed an increased expression of PR1 in BB1treated plants, confirming involvement of the SA-dependent pathway in the defensive 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nitrogen fixation, nodulation and growth ofGlycine max cv. Osumi</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">We investigated the effects of three plant growth promoting rhizobacteria (PGPR), on Biological Nitrogen Fixation (BNF), nodulation and growth promotion by soybean (Glycine max) var. Osumi plants. The strains, Aur 6, Aur 9 and Cell 4,... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147481" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">We investigated the effects of three plant growth promoting rhizobacteria (PGPR), on Biological Nitrogen Fixation (BNF), nodulation and growth promotion by soybean (Glycine max) var. Osumi plants. The strains, Aur 6, Aur 9 and Cell 4, belong to Psedomonas fluorescens, Chryseobacterium balustinum and Serratia fonticola, respectively. Inoculation modes for the PGPRs and Sinorhizobium fredii (carried out through irrigation), were examined. In the first mode, PGPRs and S. fredii were co-inoculated. In the second mode, we first inoculated S. fredii and after the PGPRs, which were added 5 or 10 days later (each inoculation being an independent treatment). In the third mode, the PGPRs were inoculated first, and the S. fredii was inoculated 5 days later. We also included treatments inoculated with only the PGPRs (one PGPR per treatment) and only with S. fredii. Plants were maintained in a greenhouse under controlled environmental conditions, and were sampled 3 months after sowing. The results obtained showed the effects of the inoculation sequence. The most significant effects on growth parameters (stem plus leaf weight and fresh root weight) were found when inoculations with PGPR and S. fredii were at different times or when we inoculated only with PGPR and the plants were watered with nitrogen. Co-inoculation had no positive effects on any parameter, probably due to competition between the PGPR and S. fredii. Our results indicate that the inoculation modes with PGPR and rhizobia play a very important role in the effects produced. Thus, although plant growth promoting rhizobacteria may interact synergistically with root-nodulating rhizobia, plant growth promoting rhizobacteria selected for one crop should be assessed for potentially hazardous effects on other crops before being used as inoculants.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147481" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="7d08fe5ea894b9eb6284e34301cafb40" rel="nofollow" data-download="{"attachment_id":44499940,"asset_id":24147481,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44499940/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-24147481">+1</span><div class="hidden js-additional-users-24147481"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/GutierrezManero">Gutiérrez Mañero</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-24147481'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-24147481').html(); 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Osumi","created_at":"2016-04-07T00:45:17.739-07:00","owner_id":46572140,"url":"https://www.academia.edu/24147481/Effects_of_inoculation_with_plant_growth_promoting_rhizobacteria_PGPRs_andSinorhizobium_fredii_on_biological_nitrogen_fixation_nodulation_and_growth_ofGlycine_max_cv_Osumi","slug":"Effects_of_inoculation_with_plant_growth_promoting_rhizobacteria_PGPRs_andSinorhizobium_fredii_on_biological_nitrogen_fixation_nodulation_and_growth_ofGlycine_max_cv_Osumi","dom_id":"work_24147481","summary":"We investigated the effects of three plant growth promoting rhizobacteria (PGPR), on Biological Nitrogen Fixation (BNF), nodulation and growth promotion by soybean (Glycine max) var. Osumi plants. The strains, Aur 6, Aur 9 and Cell 4, belong to Psedomonas fluorescens, Chryseobacterium balustinum and Serratia fonticola, respectively. Inoculation modes for the PGPRs and Sinorhizobium fredii (carried out through irrigation), were examined. In the first mode, PGPRs and S. fredii were co-inoculated. In the second mode, we first inoculated S. fredii and after the PGPRs, which were added 5 or 10 days later (each inoculation being an independent treatment). In the third mode, the PGPRs were inoculated first, and the S. fredii was inoculated 5 days later. We also included treatments inoculated with only the PGPRs (one PGPR per treatment) and only with S. fredii. Plants were maintained in a greenhouse under controlled environmental conditions, and were sampled 3 months after sowing. The results obtained showed the effects of the inoculation sequence. The most significant effects on growth parameters (stem plus leaf weight and fresh root weight) were found when inoculations with PGPR and S. fredii were at different times or when we inoculated only with PGPR and the plants were watered with nitrogen. Co-inoculation had no positive effects on any parameter, probably due to competition between the PGPR and S. fredii. Our results indicate that the inoculation modes with PGPR and rhizobia play a very important role in the effects produced. Thus, although plant growth promoting rhizobacteria may interact synergistically with root-nodulating rhizobia, plant growth promoting rhizobacteria selected for one crop should be assessed for potentially hazardous effects on other crops before being used as inoculants.","publication":"Plant and Soil","publication_with_fallback":"Plant and Soil","downloadable_attachments":[{"id":44499940,"asset_id":24147481,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44499940/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44499940/Effects_of_inoculation_with_plant_growth20160407-15248-mgfra-libre.pdf?1460017501=\u0026response-content-disposition=attachment%3B+filename%3DEffects_of_inoculation_with_plant_growth.pdf\u0026Expires=1740138714\u0026Signature=Ai9YjG6EAR1gPPvYW-pa2qJYWTbdy1SvQiIHXQQvpc1EWVmDAd72L59tvFFwm-Bedil61woQpxriuXb3DmFqwS6LRoa73mOm5YjHvaJ~0G~iSOXwim-ULqhVdXBGIWB2srtSxUox5gDizXbU1~X3q6BlaCQnlfQJPptFq00dX9lQtQihpJKd-TQI5wqXTWrWF-vuNe4Uejrh9R7yOT~RfwL3Vkt1l1UtrHPWAnGkXApj9smlPCAXZiNzvPbN70Sd-4~TH0xq90TMXrg3d3p0PY9Id-PMRaNC7N2PRr~pehdsPeYOjlSoUrVYg9F5INRvaFtgQHUUFA8tiWxiQkZyag__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44499940/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44499940/mini_magick20190214-14206-tejzm3.png?1550171691"}],"downloadable_attachments_with_full_thumbnails":[{"id":44499940,"asset_id":24147481,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44499940/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44499940/Effects_of_inoculation_with_plant_growth20160407-15248-mgfra-libre.pdf?1460017501=\u0026response-content-disposition=attachment%3B+filename%3DEffects_of_inoculation_with_plant_growth.pdf\u0026Expires=1740138715\u0026Signature=dtdI~LLsPKE8nMX9It44jdl5AoQYCA--N03KlgwAFtr7TxaV3A2xuNWfBEx~DUaUDwc1XCJCp~zakjoj4tYhF3f4n0Ol1vzZfuxSMza8JNK4mBrPEnzjvN1Ex5rIZaoWZDVTVnXF~nyuh5ZECPnh2eM1nD8JiuXMftoiYZ6JKDq9n1v4UT~jEJzSkgO4~4gYOEgSInKEurwSFfoAu9yPZEQn3aOgto0Hh3MMset6x-J-dh8Q3XpTvxap7uS~qhWZap3QkALd95usnWKhoy7mm8NmlnPBqgp91XNKcSz5Vnr~kqBZs59EqbYVBBRGSXx25RqOdOJuQsSFYil1EmLzWQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44499940/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44499940/mini_magick20190214-14206-tejzm3.png?1550171691"}],"has_pdf":true,"has_fulltext":true,"page_count":11,"ordered_authors":[{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"},{"id":46591658,"first_name":"Gutiérrez","last_name":"Mañero","domain_name":"independent","page_name":"GutierrezManero","display_name":"Gutiérrez Mañero","profile_url":"https://independent.academia.edu/GutierrezManero","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences","nofollow":true},{"id":58054,"name":"Environmental Sciences","url":"https://www.academia.edu/Documents/in/Environmental_Sciences","nofollow":true},{"id":139774,"name":"Biological Nitrogen Fixation","url":"https://www.academia.edu/Documents/in/Biological_Nitrogen_Fixation","nofollow":true},{"id":151091,"name":"Nitrogen","url":"https://www.academia.edu/Documents/in/Nitrogen","nofollow":true},{"id":188229,"name":"Soybean","url":"https://www.academia.edu/Documents/in/Soybean"},{"id":280709,"name":"Plant Growth Promoting Rhizobacteria","url":"https://www.academia.edu/Documents/in/Plant_Growth_Promoting_Rhizobacteria"},{"id":387102,"name":"Environmental Conditions","url":"https://www.academia.edu/Documents/in/Environmental_Conditions"},{"id":868872,"name":"Glycine max","url":"https://www.academia.edu/Documents/in/Glycine_max"}],"publication_year":2004,"publication_year_with_fallback":2004,"paper_rank":null,"all_time_views":64,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24147482" data-work_id="24147482" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24147482/Transgenic_tomato_plants_alter_quorum_sensing_in_plant_growth_promoting_rhizobacteria">Transgenic tomato plants alter quorum sensing in plant 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class="clearfix u-pv7x u-mb0x js-work-card work_24147493" data-work_id="24147493" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24147493/Crystal_structures_of_Ophiostoma_piceae_sterol_esterase_Structural_insights_into_activation_mechanism_and_product_release">Crystal structures of Ophiostoma piceae sterol esterase: Structural insights into activation mechanism and product release</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Sterol esterases are able to efficiently hydrolyze both sterol esters and triglycerides and to carry out synthesis reactions in the presence of organic solvents. Their high versatility makes them excellent candidates for biotechnological... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147493" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Sterol esterases are able to efficiently hydrolyze both sterol esters and triglycerides and to carry out synthesis reactions in the presence of organic solvents. Their high versatility makes them excellent candidates for biotechnological purposes. Sterol esterase from fungus Ophiostoma piceae (OPE) belongs to the family abH03.01 of the Candida rugosa lipase-like proteins. Crystal structures of OPE were solved in this study for the closed and open conformations. Enzyme activation involves a large displacement of the conserved lid, structural rearrangements of loop a16-a17, and formation of a dimer with a large opening. Three PEG molecules are placed in the active site, mimicking chains of the triglyceride substrate, demonstrating the position of the oxyanion hole and the three pockets that accommodate the sn-1, sn-2 and sn-3 fatty acids chains. One of them is an internal tunnel, connecting the active center with the outer surface of the enzyme 30 Å far from the catalytic Ser220. Based on our structural and biochemical results we propose a mechanism by which a great variety of different substrates can be hydrolyzed in OPE paving the way for the construction of new variants to improve the catalytic properties of these enzymes and their biotechnological applications.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147493" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="1aa062384d7a700d39b02ca15a10f28d" rel="nofollow" data-download="{"attachment_id":44499947,"asset_id":24147493,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44499947/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24147493 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24147493"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24147493, container: ".js-paper-rank-work_24147493", }); 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Based on our structural and biochemical results we propose a mechanism by which a great variety of different substrates can be hydrolyzed in OPE paving the way for the construction of new variants to improve the catalytic properties of these enzymes and their biotechnological applications.","publication":"Journal of Structural Biology","publication_with_fallback":"Journal of Structural 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Conformation","url":"https://www.academia.edu/Documents/in/Protein_Conformation"},{"id":1010725,"name":"Protein Binding","url":"https://www.academia.edu/Documents/in/Protein_Binding"},{"id":1030794,"name":"Hydrolysis","url":"https://www.academia.edu/Documents/in/Hydrolysis"},{"id":1681026,"name":"Biochemistry and cell biology","url":"https://www.academia.edu/Documents/in/Biochemistry_and_cell_biology"}],"publication_year":2014,"publication_year_with_fallback":2014,"paper_rank":null,"all_time_views":25,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24147505 coauthored" data-work_id="24147505" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24147505/Biotechnology_of_the_rhizosphere">Biotechnology of the rhizosphere</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">This chapter deals with the management of the rhizosphere as a living system, paying special attention to one of the three partners that define the rhizosphere: beneficial microorganisms (termed PGPR or the plant growth-promoting... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147505" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">This chapter deals with the management of the rhizosphere as a living system, paying special attention to one of the three partners that define the rhizosphere: beneficial microorganisms (termed PGPR or the plant growth-promoting rhizosphere bacteria) that inhabit it. After that, several biotechnological approaches for management of the rhizosphere will be presented. These approaches relate to environment friendly agricultural practices, the production of high-quality foods with bioactive compounds (phytonutrients), and applications in the pharmaceutical industry.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147505" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="2851711d1c9f6a4a93da8e7417861d15" rel="nofollow" data-download="{"attachment_id":44499949,"asset_id":24147505,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44499949/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-24147505">+1</span><div class="hidden js-additional-users-24147505"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://independent.academia.edu/GutierrezManero">Gutiérrez Mañero</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-24147505'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-24147505').html(); 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container.find('.percentile-widget').removeClass('hidden'); }); });</script></li><li class="js-view-count-work_24147505 InlineList-item InlineList-item--bordered hidden"><div><span><span class="js-view-count view-count u-mr2x" data-work-id="24147505"><i class="fa fa-spinner fa-spin"></i></span><script>$(function () { var workId = 24147505; window.Academia.workViewCountsFetcher.queue(workId, function (count) { var description = window.$h.commaizeInt(count) + " " + window.$h.pluralize(count, 'View'); $(".js-view-count[data-work-id=24147505]").text(description); $(".js-view-count-work_24147505").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24147505").removeClass('hidden') })</script></div></li></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24147508" data-work_id="24147508" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24147508/Protection_Against_Pathogen_and_Salt_Stress_by_Four_Plant_Growth_Promoting_Rhizobacteria_Isolated_from_Pinus_sp_on_Arabidopsis_thaliana">Protection Against Pathogen and Salt Stress by Four Plant Growth-Promoting Rhizobacteria Isolated from Pinus sp. on Arabidopsis thaliana</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">. Protection against pathogen and salt stress by four plant growth-promoting rhizobacteria isolated from Pinus sp. on Arabidopsis thaliana. Phytopathology 98:666-672. The ability of four plant growth-promoting rhizobacteria, isolated in a... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147508" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">. Protection against pathogen and salt stress by four plant growth-promoting rhizobacteria isolated from Pinus sp. on Arabidopsis thaliana. Phytopathology 98:666-672. The ability of four plant growth-promoting rhizobacteria, isolated in a previous study, to induce systemic resistance on Arabidopsis thaliana Col 0 against biotic and abiotic stress was evaluated. All the bacteria enhanced protection against the foliar pathogen Pseudomonas syringae DC3000 and increased plant tolerance to salt stress (NaCl 60 mM). Bacillus sp. strain L81 and Arthrobacter oxidans strain BB1 performed best with a decrease in the disease index of 61.2 and 52.3%, respectively, and a reduction in the mortality due to salt stress of 72.4 and 57.8%, respectively. Additionally, significant differences were found in growth and photosynthesis, again, L81 and BB1 performed best either in normal or under stress conditions. In order to elucidate the pathway elicited by these two strains to induce systemic resistance, experiments with the transgenic line of Arabidopsis thaliana NahG (defective in salicylic acid [SA]) and with the jar1 mutant (defective in jasmonic acid) were carried out. Results showed that the SA-dependent pathway was involved in the defense response induced by strains L81 and BB1. Results from quantitative reverse transcription-polymerase chain reaction analysis of the PR1 gene, related to the SA-dependent pathway and the PDF1.2 gene related to the SAindependent pathway, showed an increased expression of PR1 in BB1treated plants, confirming involvement of the SA-dependent pathway in the defensive response.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147508" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="4b9974ee4c9444da94a82c4fb253863e" rel="nofollow" data-download="{"attachment_id":44499950,"asset_id":24147508,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44499950/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24147508 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24147508"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24147508, container: ".js-paper-rank-work_24147508", }); 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Protection against pathogen and salt stress by four plant growth-promoting rhizobacteria isolated from Pinus sp. on Arabidopsis thaliana. Phytopathology 98:666-672. The ability of four plant growth-promoting rhizobacteria, isolated in a previous study, to induce systemic resistance on Arabidopsis thaliana Col 0 against biotic and abiotic stress was evaluated. All the bacteria enhanced protection against the foliar pathogen Pseudomonas syringae DC3000 and increased plant tolerance to salt stress (NaCl 60 mM). Bacillus sp. strain L81 and Arthrobacter oxidans strain BB1 performed best with a decrease in the disease index of 61.2 and 52.3%, respectively, and a reduction in the mortality due to salt stress of 72.4 and 57.8%, respectively. Additionally, significant differences were found in growth and photosynthesis, again, L81 and BB1 performed best either in normal or under stress conditions. In order to elucidate the pathway elicited by these two strains to induce systemic resistance, experiments with the transgenic line of Arabidopsis thaliana NahG (defective in salicylic acid [SA]) and with the jar1 mutant (defective in jasmonic acid) were carried out. Results showed that the SA-dependent pathway was involved in the defense response induced by strains L81 and BB1. Results from quantitative reverse transcription-polymerase chain reaction analysis of the PR1 gene, related to the SA-dependent pathway and the PDF1.2 gene related to the SAindependent pathway, showed an increased expression of PR1 in BB1treated plants, confirming involvement of the SA-dependent pathway in the defensive response.","publication":"Phytopathology","publication_with_fallback":"Phytopathology","downloadable_attachments":[{"id":44499950,"asset_id":24147508,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44499950/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44499950/Protection_against_pathogen_and_salt_str20160407-3060-win07a-libre.pdf?1460017501=\u0026response-content-disposition=attachment%3B+filename%3DProtection_Against_Pathogen_and_Salt_Str.pdf\u0026Expires=1740138716\u0026Signature=G~eHUghMiNax-Z7S2T8tw9J1Z8hcUuiMG3y66t7RGdz~QIEfZOoh7OiqSww1ErsaME~v4Tp6-3tOMCMWnD4f4TDTJ~kFx9gu5NLelGrUlqoUQBr7fiIkpgH3PwiaCAlNo9s-hMvFQoxF5mRpIwUD47JWg2zMxGHPmlpZ9HIK3TGI~QQNcxDrPy4VqIYqQIyvR5WjgQzMl68ovwx8tyISc7VtIh3jpgOSL-2zgXpnyb2q8-uyln99zChovaJx23oHYpm5DniHTW6mihqv5hkIohF5PeYCRbAh3o1UeqsjoqU~d6x-7Oj3TOiq5BJGFNFwzIlXJJtscHunsiTNBNmHVg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44499950/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44499950/mini_magick20190214-22746-81csjt.png?1550171705"}],"downloadable_attachments_with_full_thumbnails":[{"id":44499950,"asset_id":24147508,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44499950/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44499950/Protection_against_pathogen_and_salt_str20160407-3060-win07a-libre.pdf?1460017501=\u0026response-content-disposition=attachment%3B+filename%3DProtection_Against_Pathogen_and_Salt_Str.pdf\u0026Expires=1740138716\u0026Signature=G~eHUghMiNax-Z7S2T8tw9J1Z8hcUuiMG3y66t7RGdz~QIEfZOoh7OiqSww1ErsaME~v4Tp6-3tOMCMWnD4f4TDTJ~kFx9gu5NLelGrUlqoUQBr7fiIkpgH3PwiaCAlNo9s-hMvFQoxF5mRpIwUD47JWg2zMxGHPmlpZ9HIK3TGI~QQNcxDrPy4VqIYqQIyvR5WjgQzMl68ovwx8tyISc7VtIh3jpgOSL-2zgXpnyb2q8-uyln99zChovaJx23oHYpm5DniHTW6mihqv5hkIohF5PeYCRbAh3o1UeqsjoqU~d6x-7Oj3TOiq5BJGFNFwzIlXJJtscHunsiTNBNmHVg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44499950/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44499950/mini_magick20190214-22746-81csjt.png?1550171705"}],"has_pdf":true,"has_fulltext":true,"page_count":7,"ordered_authors":[{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":159,"name":"Microbiology","url":"https://www.academia.edu/Documents/in/Microbiology","nofollow":true},{"id":2512,"name":"Phytopathology","url":"https://www.academia.edu/Documents/in/Phytopathology","nofollow":true},{"id":5345,"name":"Photosynthesis","url":"https://www.academia.edu/Documents/in/Photosynthesis","nofollow":true},{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology","nofollow":true},{"id":9729,"name":"Stress","url":"https://www.academia.edu/Documents/in/Stress"},{"id":20285,"name":"Salt Stress","url":"https://www.academia.edu/Documents/in/Salt_Stress"},{"id":24706,"name":"Innate immunity","url":"https://www.academia.edu/Documents/in/Innate_immunity"},{"id":37434,"name":"Quantitative analysis","url":"https://www.academia.edu/Documents/in/Quantitative_analysis"},{"id":41553,"name":"Arabidopsis 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Chloride","url":"https://www.academia.edu/Documents/in/Sodium_Chloride"},{"id":2281154,"name":"Terpenoid","url":"https://www.academia.edu/Documents/in/Terpenoid"}],"publication_year":2008,"publication_year_with_fallback":2008,"paper_rank":null,"all_time_views":8,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24147868" data-work_id="24147868" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24147868/Estimation_of_bacterial_diversity_using_next_generation_sequencing_of_16S_rDNA_a_comparison_of_different_workflows">Estimation of bacterial diversity using next generation sequencing of 16S rDNA: a comparison of different workflows</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Background: Next generation sequencing (NGS) enables a more comprehensive analysis of bacterial diversity from complex environmental samples. NGS data can be analysed using a variety of workflows. We test several simple and complex... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147868" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background: Next generation sequencing (NGS) enables a more comprehensive analysis of bacterial diversity from complex environmental samples. NGS data can be analysed using a variety of workflows. We test several simple and complex workflows, including frequently used as well as recently published tools, and report on their respective accuracy and efficiency under various conditions covering different sequence lengths, number of sequences and real world experimental data from rhizobacterial populations of glyphosate-tolerant maize treated or untreated with two different herbicides representative of differential diversity studies. Results: Alignment and distance calculations affect OTU estimations, and multiple sequence alignment exerts a major impact on the computational time needed. Generally speaking, most of the analyses produced consistent results that may be used to assess differential diversity changes, however, dataset characteristics dictate which workflow should be preferred in each case. Conclusions: When estimating bacterial diversity, ESPRIT as well as the web-based workflow, RDP pyrosequencing pipeline, produced good results in all circumstances, however, its computational requirements can make methodcombination workflows more attractive, depending on sequence variability, number and length.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147868" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="79f07c8c1e295cb511f3ff511993d22f" rel="nofollow" data-download="{"attachment_id":44500153,"asset_id":24147868,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44500153/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24147868 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24147868"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24147868, container: ".js-paper-rank-work_24147868", }); 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NGS data can be analysed using a variety of workflows. We test several simple and complex workflows, including frequently used as well as recently published tools, and report on their respective accuracy and efficiency under various conditions covering different sequence lengths, number of sequences and real world experimental data from rhizobacterial populations of glyphosate-tolerant maize treated or untreated with two different herbicides representative of differential diversity studies. Results: Alignment and distance calculations affect OTU estimations, and multiple sequence alignment exerts a major impact on the computational time needed. Generally speaking, most of the analyses produced consistent results that may be used to assess differential diversity changes, however, dataset characteristics dictate which workflow should be preferred in each case. Conclusions: When estimating bacterial diversity, ESPRIT as well as the web-based workflow, RDP pyrosequencing pipeline, produced good results in all circumstances, however, its computational requirements can make methodcombination workflows more attractive, depending on sequence variability, number and length.","publication":"BMC Bioinformatics","publication_with_fallback":"BMC Bioinformatics","downloadable_attachments":[{"id":44500153,"asset_id":24147868,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44500153/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44500153/Estimation_of_bacterial_diversity_using_20160407-15263-wl2t2d-libre.pdf?1460018087=\u0026response-content-disposition=attachment%3B+filename%3DEstimation_of_bacterial_diversity_using.pdf\u0026Expires=1740138716\u0026Signature=DYm4S~Xb1R5IRHYAdmU1Cw31cy4E1TdZ7eqQy~HtR5l1SgIwM4~NFSUcIBhrKLuIULYLxDSlpeX4C7ROX4k8RIT6HfcZKow0Sl2OHDedwoMHNZYglCFghYhm3ghyIVdzjrIMbEtmVp8MVZ8CpXqNU4V8FuIUBlX~ItsejG7VoHafskNnpAewR9u-WINAm-xGNNCz38z~hlSk6-vdjZcP~MF7UzvIL9P-zsirIJr9FUQjlVSv~6kudVqZj0Lm0o3FDn5F0KUFX2kj3nQ4S6QR-vH0QnIoAwKoVOSho5WlGc80PaBX5IpM71vMviAfT5xqhN77mv1p2IYkR2-H~Qp2DA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44500153/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44500153/mini_magick20190214-13688-r0w1uy.png?1550171665"}],"downloadable_attachments_with_full_thumbnails":[{"id":44500153,"asset_id":24147868,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44500153/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44500153/Estimation_of_bacterial_diversity_using_20160407-15263-wl2t2d-libre.pdf?1460018087=\u0026response-content-disposition=attachment%3B+filename%3DEstimation_of_bacterial_diversity_using.pdf\u0026Expires=1740138716\u0026Signature=DYm4S~Xb1R5IRHYAdmU1Cw31cy4E1TdZ7eqQy~HtR5l1SgIwM4~NFSUcIBhrKLuIULYLxDSlpeX4C7ROX4k8RIT6HfcZKow0Sl2OHDedwoMHNZYglCFghYhm3ghyIVdzjrIMbEtmVp8MVZ8CpXqNU4V8FuIUBlX~ItsejG7VoHafskNnpAewR9u-WINAm-xGNNCz38z~hlSk6-vdjZcP~MF7UzvIL9P-zsirIJr9FUQjlVSv~6kudVqZj0Lm0o3FDn5F0KUFX2kj3nQ4S6QR-vH0QnIoAwKoVOSho5WlGc80PaBX5IpM71vMviAfT5xqhN77mv1p2IYkR2-H~Qp2DA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44500153/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44500153/mini_magick20190214-13688-r0w1uy.png?1550171665"}],"has_pdf":true,"has_fulltext":true,"page_count":11,"ordered_authors":[{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":10523,"name":"Workflow","url":"https://www.academia.edu/Documents/in/Workflow","nofollow":true},{"id":17825,"name":"Biodiversity","url":"https://www.academia.edu/Documents/in/Biodiversity","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences","nofollow":true},{"id":80414,"name":"Mathematical Sciences","url":"https://www.academia.edu/Documents/in/Mathematical_Sciences","nofollow":true},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria"},{"id":123230,"name":"Regression Analysis","url":"https://www.academia.edu/Documents/in/Regression_Analysis"},{"id":146416,"name":"BMC Bioinformatics","url":"https://www.academia.edu/Documents/in/BMC_Bioinformatics"},{"id":231547,"name":"Soil Microbiology","url":"https://www.academia.edu/Documents/in/Soil_Microbiology"}],"publication_year":2011,"publication_year_with_fallback":2011,"paper_rank":null,"all_time_views":12,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24147870" data-work_id="24147870" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24147870/Relative_Effect_of_Glyphosate_on_Glyphosate_Tolerant_Maize_Rhizobacterial_Communities_is_Not_Altered_by_Soil_Properties">Relative Effect of Glyphosate on Glyphosate-Tolerant Maize Rhizobacterial Communities is Not Altered by Soil Properties</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">The rhizobacterial composition varies according to the soil properties. To test if the effect of herbicides on the rhizobacterial communities of genetically modified NK603 glyphosate-tolerant maize varies according to different soil... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147870" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">The rhizobacterial composition varies according to the soil properties. To test if the effect of herbicides on the rhizobacterial communities of genetically modified NK603 glyphosate-tolerant maize varies according to different soil locations, a comparison was made between the effects of glyphosate (Roundup Plus), a post-emergence applied herbicide, and a pre-emergence applied herbicide (GTZ) versus untreated soil. The potential effect was monitored by direct amplification, cloning, and sequencing of the soil DNA encoding 16S rRNA, and high-throughput DNA pyrosequencing of the bacterial DNA coding for the 16S rRNA hypervariable V6 region. The results obtained using three different methods to analyze the herbicide effect on the rhizobacterial communities of genetically modified NK603 maize were comparable to those previously obtained when glyphosate-tolerant maize was grown in soil with different characteristics. Both herbicides decreased the bacterial diversity in the rhizosphere, with Actinobacteria being the taxonomic group most affected. The results suggest that both herbicides affected the structure of the maize rhizobacterial community, but glyphosate was environmentally less aggressive.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147870" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="e2b342f00f108d9c9e8c614edceec4dc" rel="nofollow" data-download="{"attachment_id":44500156,"asset_id":24147870,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44500156/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24147870 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24147870"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24147870, container: ".js-paper-rank-work_24147870", }); 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$(".js-view-count[data-work-id=24147870]").text(description); $(".js-view-count-work_24147870").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24147870").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24147870"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">12</a>  </div><span class="InlineList-item-text u-textTruncate u-pl10x"><a class="InlineList-item-text" data-has-card-for-ri="923" rel="nofollow" href="https://www.academia.edu/Documents/in/Technology">Technology</a>, <script data-card-contents-for-ri="923" type="text/json">{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="47884" rel="nofollow" href="https://www.academia.edu/Documents/in/Biological_Sciences">Biological Sciences</a>, <script data-card-contents-for-ri="47884" type="text/json">{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="54433" rel="nofollow" href="https://www.academia.edu/Documents/in/Phylogeny">Phylogeny</a>, <script data-card-contents-for-ri="54433" type="text/json">{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="73035" rel="nofollow" href="https://www.academia.edu/Documents/in/Microbiology_and_Biotechnology">Microbiology and Biotechnology</a><script data-card-contents-for-ri="73035" type="text/json">{"id":73035,"name":"Microbiology and Biotechnology","url":"https://www.academia.edu/Documents/in/Microbiology_and_Biotechnology","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24147870]'), work: {"id":24147870,"title":"Relative Effect of Glyphosate on Glyphosate-Tolerant Maize Rhizobacterial Communities is Not Altered by Soil Properties","created_at":"2016-04-07T00:51:57.180-07:00","owner_id":46572140,"url":"https://www.academia.edu/24147870/Relative_Effect_of_Glyphosate_on_Glyphosate_Tolerant_Maize_Rhizobacterial_Communities_is_Not_Altered_by_Soil_Properties","slug":"Relative_Effect_of_Glyphosate_on_Glyphosate_Tolerant_Maize_Rhizobacterial_Communities_is_Not_Altered_by_Soil_Properties","dom_id":"work_24147870","summary":"The rhizobacterial composition varies according to the soil properties. To test if the effect of herbicides on the rhizobacterial communities of genetically modified NK603 glyphosate-tolerant maize varies according to different soil locations, a comparison was made between the effects of glyphosate (Roundup Plus), a post-emergence applied herbicide, and a pre-emergence applied herbicide (GTZ) versus untreated soil. The potential effect was monitored by direct amplification, cloning, and sequencing of the soil DNA encoding 16S rRNA, and high-throughput DNA pyrosequencing of the bacterial DNA coding for the 16S rRNA hypervariable V6 region. The results obtained using three different methods to analyze the herbicide effect on the rhizobacterial communities of genetically modified NK603 maize were comparable to those previously obtained when glyphosate-tolerant maize was grown in soil with different characteristics. Both herbicides decreased the bacterial diversity in the rhizosphere, with Actinobacteria being the taxonomic group most affected. The results suggest that both herbicides affected the structure of the maize rhizobacterial community, but glyphosate was environmentally less aggressive.","publication":"Journal of Microbiology and Biotechnology","publication_with_fallback":"Journal of Microbiology and Biotechnology","downloadable_attachments":[{"id":44500156,"asset_id":24147870,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44500156/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44500156/Relative_effect_of_glyphosate_on_glyphos20160407-30460-13984zs-libre.pdf?1460018088=\u0026response-content-disposition=attachment%3B+filename%3DRelative_Effect_of_Glyphosate_on_Glyphos.pdf\u0026Expires=1740124708\u0026Signature=Dbk2CmnppeFoB6iQvr9sMFn25ooKaPyi1K~~X8WNPeBvfAkOggYDd3reK2MVMgLNOC4uhTeWYAdbDHsM63BQh5m-OTGBjjeFKp07w4tmxHwlRCCFyWSPB8trIIMY-ORIdQ3Qh8EAaC3~NKUnwOmEUPx3JMCbhluIGkM4-wKOT4tvvb2ib~hwcThQpImC2exHzpDummztSZ5I71edSq9wU5y4EFDT7FJNiLKxp1TVtPPBb70bG4zKwV53MS2iPqwJ-RNWKHqU29aDH87YcK7PAIsKXQ60EuC0~D6cEVAPNzRaWNyI37rDRRW1WVmdQg3xK~q56moT8mIwzkPc3Tkbqg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44500156/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44500156/mini_magick20190214-14203-t8tlcx.png?1550171663"}],"downloadable_attachments_with_full_thumbnails":[{"id":44500156,"asset_id":24147870,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44500156/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44500156/Relative_effect_of_glyphosate_on_glyphos20160407-30460-13984zs-libre.pdf?1460018088=\u0026response-content-disposition=attachment%3B+filename%3DRelative_Effect_of_Glyphosate_on_Glyphos.pdf\u0026Expires=1740124708\u0026Signature=Dbk2CmnppeFoB6iQvr9sMFn25ooKaPyi1K~~X8WNPeBvfAkOggYDd3reK2MVMgLNOC4uhTeWYAdbDHsM63BQh5m-OTGBjjeFKp07w4tmxHwlRCCFyWSPB8trIIMY-ORIdQ3Qh8EAaC3~NKUnwOmEUPx3JMCbhluIGkM4-wKOT4tvvb2ib~hwcThQpImC2exHzpDummztSZ5I71edSq9wU5y4EFDT7FJNiLKxp1TVtPPBb70bG4zKwV53MS2iPqwJ-RNWKHqU29aDH87YcK7PAIsKXQ60EuC0~D6cEVAPNzRaWNyI37rDRRW1WVmdQg3xK~q56moT8mIwzkPc3Tkbqg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44500156/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44500156/mini_magick20190214-14203-t8tlcx.png?1550171663"}],"has_pdf":true,"has_fulltext":true,"page_count":7,"ordered_authors":[{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":923,"name":"Technology","url":"https://www.academia.edu/Documents/in/Technology","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences","nofollow":true},{"id":54433,"name":"Phylogeny","url":"https://www.academia.edu/Documents/in/Phylogeny","nofollow":true},{"id":73035,"name":"Microbiology and Biotechnology","url":"https://www.academia.edu/Documents/in/Microbiology_and_Biotechnology","nofollow":true},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria"},{"id":131237,"name":"Cluster Analysis","url":"https://www.academia.edu/Documents/in/Cluster_Analysis"},{"id":223073,"name":"Herbicides","url":"https://www.academia.edu/Documents/in/Herbicides"},{"id":231547,"name":"Soil Microbiology","url":"https://www.academia.edu/Documents/in/Soil_Microbiology"},{"id":231549,"name":"Rhizosphere","url":"https://www.academia.edu/Documents/in/Rhizosphere"},{"id":370863,"name":"Zea mays","url":"https://www.academia.edu/Documents/in/Zea_mays"},{"id":712543,"name":"Glycine","url":"https://www.academia.edu/Documents/in/Glycine"},{"id":834420,"name":"Biota","url":"https://www.academia.edu/Documents/in/Biota"}],"publication_year":2012,"publication_year_with_fallback":2012,"paper_rank":null,"all_time_views":12,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24147872" data-work_id="24147872" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24147872/Potential_Accumulative_Effect_of_the_Herbicide_Glyphosate_on_Glyphosate_Tolerant_Maize_Rhizobacterial_Communities_over_a_Three_Year_Cultivation_Period">Potential Accumulative Effect of the Herbicide Glyphosate on Glyphosate-Tolerant Maize Rhizobacterial Communities over a Three-Year Cultivation Period</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Background: Glyphosate is a herbicide that is liable to be used in the extensive cultivation of glyphosate-tolerant cultivars. The potential accumulation of the relative effect of glyphosate on the rhizobacterial communities of... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147872" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background: Glyphosate is a herbicide that is liable to be used in the extensive cultivation of glyphosate-tolerant cultivars. The potential accumulation of the relative effect of glyphosate on the rhizobacterial communities of glyphosate-tolerant maize has been monitored over a period of three years.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147872" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="ce3106eb04cdf003705787c430c6dae6" rel="nofollow" data-download="{"attachment_id":44500154,"asset_id":24147872,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44500154/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24147872 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24147872"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24147872, container: ".js-paper-rank-work_24147872", }); 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$(".js-view-count[data-work-id=24147872]").text(description); $(".js-view-count-work_24147872").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24147872").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24147872"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">6</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="220780" rel="nofollow" href="https://www.academia.edu/Documents/in/PLoS_one">PLoS one</a>, <script data-card-contents-for-ri="220780" type="text/json">{"id":220780,"name":"PLoS one","url":"https://www.academia.edu/Documents/in/PLoS_one","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="223073" rel="nofollow" href="https://www.academia.edu/Documents/in/Herbicides">Herbicides</a>, <script data-card-contents-for-ri="223073" type="text/json">{"id":223073,"name":"Herbicides","url":"https://www.academia.edu/Documents/in/Herbicides","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="370863" rel="nofollow" href="https://www.academia.edu/Documents/in/Zea_mays">Zea mays</a><script data-card-contents-for-ri="370863" type="text/json">{"id":370863,"name":"Zea mays","url":"https://www.academia.edu/Documents/in/Zea_mays","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24147872]'), work: {"id":24147872,"title":"Potential Accumulative Effect of the Herbicide Glyphosate on Glyphosate-Tolerant Maize Rhizobacterial Communities over a Three-Year Cultivation Period","created_at":"2016-04-07T00:51:57.521-07:00","owner_id":46572140,"url":"https://www.academia.edu/24147872/Potential_Accumulative_Effect_of_the_Herbicide_Glyphosate_on_Glyphosate_Tolerant_Maize_Rhizobacterial_Communities_over_a_Three_Year_Cultivation_Period","slug":"Potential_Accumulative_Effect_of_the_Herbicide_Glyphosate_on_Glyphosate_Tolerant_Maize_Rhizobacterial_Communities_over_a_Three_Year_Cultivation_Period","dom_id":"work_24147872","summary":"Background: Glyphosate is a herbicide that is liable to be used in the extensive cultivation of glyphosate-tolerant cultivars. 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The potential accumulation of the relative effect of the transgenic modification and the cry toxin on the rhizobacterial... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24147874" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Background: Bt-maize is a transgenic variety of maize expressing the Cry toxin from Bacillus turingiensis. The potential accumulation of the relative effect of the transgenic modification and the cry toxin on the rhizobacterial communities of Btmaize has been monitored over a period of four years.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24147874" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="778d67d3487ea1476a4535be1fb7ece8" rel="nofollow" data-download="{"attachment_id":44500155,"asset_id":24147874,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44500155/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24147874 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24147874"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24147874, container: ".js-paper-rank-work_24147874", }); 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$(".js-view-count[data-work-id=24147874]").text(description); $(".js-view-count-work_24147874").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24147874").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24147874"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">8</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="113903" rel="nofollow" href="https://www.academia.edu/Documents/in/Bacteria">Bacteria</a>, <script data-card-contents-for-ri="113903" type="text/json">{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="131237" rel="nofollow" href="https://www.academia.edu/Documents/in/Cluster_Analysis">Cluster Analysis</a>, <script data-card-contents-for-ri="131237" type="text/json">{"id":131237,"name":"Cluster Analysis","url":"https://www.academia.edu/Documents/in/Cluster_Analysis","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="202399" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Roots">Plant Roots</a><script data-card-contents-for-ri="202399" type="text/json">{"id":202399,"name":"Plant Roots","url":"https://www.academia.edu/Documents/in/Plant_Roots","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24147874]'), work: {"id":24147874,"title":"Effect of Cry1Ab Protein on Rhizobacterial Communities of Bt-Maize over a Four-Year Cultivation Period","created_at":"2016-04-07T00:51:57.834-07:00","owner_id":46572140,"url":"https://www.academia.edu/24147874/Effect_of_Cry1Ab_Protein_on_Rhizobacterial_Communities_of_Bt_Maize_over_a_Four_Year_Cultivation_Period","slug":"Effect_of_Cry1Ab_Protein_on_Rhizobacterial_Communities_of_Bt_Maize_over_a_Four_Year_Cultivation_Period","dom_id":"work_24147874","summary":"Background: Bt-maize is a transgenic variety of maize expressing the Cry toxin from Bacillus turingiensis. The potential accumulation of the relative effect of the transgenic modification and the cry toxin on the rhizobacterial communities of Btmaize has been monitored over a period of four years.","publication":"PLoS ONE","publication_with_fallback":"PLoS ONE","downloadable_attachments":[{"id":44500155,"asset_id":24147874,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44500155/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44500155/Effect_of_Cry1Ab_Protein_on_Rhizobacteri20160407-9844-1og0yyn-libre.pdf?1460018086=\u0026response-content-disposition=attachment%3B+filename%3DEffect_of_Cry1Ab_Protein_on_Rhizobacteri.pdf\u0026Expires=1740138716\u0026Signature=bKhnp5Ss7B08D9U0WJ7BaOmgeZSVbVZLWyN5JqVJwpGU9CIh888aPV~O2v7jXzav5bPNpPBo6yYkVArFqlvOJRzNol4GCouYanMPHw84OOzGF0Q6iyYVNlVv~ypwcBGZjX2MlIwGr1nbo6io9pJfYbRbE-jlH1vkXBo6EcuFSsf6LYeazaR9owAEJJtJvO47okNoG-bl2tY85jeXebBy~PgH8HNg0MLSrtOZySCYUD~BFKB-a27IO4SRJetKUAia-rsaD5NFdPXgA7u8U53aDAjdPWEUjouidwVKOqtjmBsi3lq7aZ8tMrpbr5JDJBoFEj3KNdm7a3yUA6OpDbbiJA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44500155/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44500155/mini_magick20220707-1209-1kv33ga.png?1657260727"}],"downloadable_attachments_with_full_thumbnails":[{"id":44500155,"asset_id":24147874,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44500155/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44500155/Effect_of_Cry1Ab_Protein_on_Rhizobacteri20160407-9844-1og0yyn-libre.pdf?1460018086=\u0026response-content-disposition=attachment%3B+filename%3DEffect_of_Cry1Ab_Protein_on_Rhizobacteri.pdf\u0026Expires=1740138716\u0026Signature=bKhnp5Ss7B08D9U0WJ7BaOmgeZSVbVZLWyN5JqVJwpGU9CIh888aPV~O2v7jXzav5bPNpPBo6yYkVArFqlvOJRzNol4GCouYanMPHw84OOzGF0Q6iyYVNlVv~ypwcBGZjX2MlIwGr1nbo6io9pJfYbRbE-jlH1vkXBo6EcuFSsf6LYeazaR9owAEJJtJvO47okNoG-bl2tY85jeXebBy~PgH8HNg0MLSrtOZySCYUD~BFKB-a27IO4SRJetKUAia-rsaD5NFdPXgA7u8U53aDAjdPWEUjouidwVKOqtjmBsi3lq7aZ8tMrpbr5JDJBoFEj3KNdm7a3yUA6OpDbbiJA__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44500155/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44500155/mini_magick20220707-1209-1kv33ga.png?1657260727"}],"has_pdf":true,"has_fulltext":true,"page_count":7,"ordered_authors":[{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary","nofollow":true},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria","nofollow":true},{"id":131237,"name":"Cluster Analysis","url":"https://www.academia.edu/Documents/in/Cluster_Analysis","nofollow":true},{"id":202399,"name":"Plant Roots","url":"https://www.academia.edu/Documents/in/Plant_Roots","nofollow":true},{"id":220780,"name":"PLoS one","url":"https://www.academia.edu/Documents/in/PLoS_one"},{"id":231547,"name":"Soil Microbiology","url":"https://www.academia.edu/Documents/in/Soil_Microbiology"},{"id":370863,"name":"Zea mays","url":"https://www.academia.edu/Documents/in/Zea_mays"},{"id":1451915,"name":"Endotoxins","url":"https://www.academia.edu/Documents/in/Endotoxins"}],"publication_year":2012,"publication_year_with_fallback":2012,"paper_rank":null,"all_time_views":5,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24148653" data-work_id="24148653" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24148653/Differences_in_cell_wall_polysaccharide_composition_between_embryogenic_and_non_embryogenic_calli_of_Medicago_arborea_L">Differences in cell wall polysaccharide composition between embryogenic and non-embryogenic calli of Medicago arborea L</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Analysis of cell wall polysaccharide composition of embryogenic and non-embryogenic calli obtained from hypocotyl and petiole explants from Medicago arborea L. revealed significant differences. For calli induced from both hypocotyls and... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24148653" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Analysis of cell wall polysaccharide composition of embryogenic and non-embryogenic calli obtained from hypocotyl and petiole explants from Medicago arborea L. revealed significant differences. For calli induced from both hypocotyls and petioles, levels of total sugars, pectins, and hemicelluloses were higher in embryogenic than in non-embryogenic calli. Whereas in the residual cellulose fraction, the highest levels of sugar were detected in non-embryogenic calli. When comparing the two donor sources of callus explants, the highest total sugar levels were detected in embryogenic calli induced from petioles, mainly in the pectin fraction and to a lesser extent in the hemicellulose fraction. Moreover, analysis of uronic acids revealed higher levels in embryogenic calli, primarily in the pectin fraction. Analysis of those sugars associated with cell walls of calli suggested that these polysaccharides consisted of pectic polysaccharides and glucans, and that their levels were higher in embryogenic than non-embryogenic calli.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24148653" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="c3c8380cac3d6b86f1c6b76b2e2c96ad" rel="nofollow" data-download="{"attachment_id":44500587,"asset_id":24148653,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44500587/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24148653 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24148653"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24148653, container: ".js-paper-rank-work_24148653", }); 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$(".js-view-count[data-work-id=24148653]").text(description); $(".js-view-count-work_24148653").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24148653").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24148653"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">2</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="5541" rel="nofollow" href="https://www.academia.edu/Documents/in/Plant_Biology">Plant Biology</a>, <script data-card-contents-for-ri="5541" type="text/json">{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="837135" rel="nofollow" href="https://www.academia.edu/Documents/in/Cell_Wall">Cell Wall</a><script data-card-contents-for-ri="837135" type="text/json">{"id":837135,"name":"Cell Wall","url":"https://www.academia.edu/Documents/in/Cell_Wall","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24148653]'), work: {"id":24148653,"title":"Differences in cell wall polysaccharide composition between embryogenic and non-embryogenic calli of Medicago arborea L","created_at":"2016-04-07T01:09:38.621-07:00","owner_id":46572140,"url":"https://www.academia.edu/24148653/Differences_in_cell_wall_polysaccharide_composition_between_embryogenic_and_non_embryogenic_calli_of_Medicago_arborea_L","slug":"Differences_in_cell_wall_polysaccharide_composition_between_embryogenic_and_non_embryogenic_calli_of_Medicago_arborea_L","dom_id":"work_24148653","summary":"Analysis of cell wall polysaccharide composition of embryogenic and non-embryogenic calli obtained from hypocotyl and petiole explants from Medicago arborea L. revealed significant differences. 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Analysis of those sugars associated with cell walls of calli suggested that these polysaccharides consisted of pectic polysaccharides and glucans, and that their levels were higher in embryogenic than non-embryogenic calli.","publication":"Plant Cell, Tissue and Organ Culture (PCTOC)","publication_with_fallback":"Plant Cell, Tissue and Organ Culture (PCTOC)","downloadable_attachments":[{"id":44500587,"asset_id":24148653,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44500587/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44500587/Differences_in_cell_wall_polysaccharide_20160407-30460-5kgt8x-libre.pdf?1460019192=\u0026response-content-disposition=attachment%3B+filename%3DDifferences_in_cell_wall_polysaccharide.pdf\u0026Expires=1740138716\u0026Signature=YQaVkRkI0UaO7Oif18GrnMUx3nYmuwyZ9npbTH9yiQ~NJDFO3OFJ~Jm~-OVT4ET9K64C5zeMIEDCSVAoH06PIUeR401YmqDaxAaiVDHeHqwE4BGRCHIxacWmHll6iaP2dEZ6G0an5LArp8WnktMzu4ScX~12uFonFum2Uapn4hiq1ZNsx1kp5d3Sa1LHIlr3YZRmZSSj-ufkVYbn4y4P7F6S02rJRMpG8~~-JUtK6dpewXRBrMUJMB3B-tqEATZdtHbW1mkjiPemWdN9vLlIw5iZzko~B~mebB9JZix--CbMIo86hpOprvKb0AGb95YhOwWxfzwk4NjM~62BzSSmwQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44500587/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44500587/mini_magick20190214-13687-zn5cii.png?1550171605"}],"downloadable_attachments_with_full_thumbnails":[{"id":44500587,"asset_id":24148653,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/44500587/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/44500587/Differences_in_cell_wall_polysaccharide_20160407-30460-5kgt8x-libre.pdf?1460019192=\u0026response-content-disposition=attachment%3B+filename%3DDifferences_in_cell_wall_polysaccharide.pdf\u0026Expires=1740138717\u0026Signature=ILUDl5n5UvEQyvZpRFMz9hCZoNBEoyuMzohTjpAQWRy-L8~l2iYwD1eGddUbQsaT13h~ZdThJ6jdWy7~B9ZG3zBeWnsNlCUZJjHCt0eH254-HOqiz4TLdbshY8mspOby6nHh5PneOvdAo4NcrHl4Jv3kI9IXpAPNBSR2YVAuaNjPYsYHYku-EBahK~BbDR78x17ru3mbHcDnNigFSYmQlq4PVWPMWoiLFbP5JwWUC55pFcr6pPFA0pSfjy~YBVw6mRuvdBGBpqHnGbSFecqx6YAHtpmbp9CyZuKpcFYlP~gW5zlcOAwOFI5tQd8c3yQ2gjd8rvAz1xXB7ipBDh0PFQ__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/44500587/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/44500587/mini_magick20190214-13687-zn5cii.png?1550171605"}],"has_pdf":true,"has_fulltext":true,"page_count":7,"ordered_authors":[{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":5541,"name":"Plant Biology","url":"https://www.academia.edu/Documents/in/Plant_Biology","nofollow":true},{"id":837135,"name":"Cell Wall","url":"https://www.academia.edu/Documents/in/Cell_Wall","nofollow":true}],"publication_year":2009,"publication_year_with_fallback":2009,"paper_rank":null,"all_time_views":21,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_24148656" data-work_id="24148656" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/24148656/Quorum_sensing_mechanisms_mediated_by_farnesol_in_Ophiostoma_piceae_its_effect_on_the_secretion_of_sterol_esterase">Quorum sensing mechanisms mediated by farnesol in Ophiostoma piceae : its effect on the secretion of sterol esterase</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Ophiostoma piceae CECT 20416 is a dimorphic wood-staining fungus able to produce 17 an extracellular sterol-esterase/lipase (OPE) with great biotechnological interest. In this work 18 we have studied the morphological change from yeast to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_24148656" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Ophiostoma piceae CECT 20416 is a dimorphic wood-staining fungus able to produce 17 an extracellular sterol-esterase/lipase (OPE) with great biotechnological interest. In this work 18 we have studied the morphological change from yeast to hyphae of this fungus, associated to 19 the cell-density related mechanism known as quorum sensing (QS), and how this affects the 20 secretion of OPE. 21</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/24148656" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="224d84dc1e314b8d8ef0bb75a396ea24" rel="nofollow" data-download="{"attachment_id":44500588,"asset_id":24148656,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44500588/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="46572140" href="https://csic.academia.edu/JorgeBarriuso">Jorge Barriuso</a><script data-card-contents-for-user="46572140" type="text/json">{"id":46572140,"first_name":"Jorge","last_name":"Barriuso","domain_name":"csic","page_name":"JorgeBarriuso","display_name":"Jorge Barriuso","profile_url":"https://csic.academia.edu/JorgeBarriuso","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_24148656 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="24148656"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 24148656, container: ".js-paper-rank-work_24148656", }); 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$(".js-view-count[data-work-id=24148656]").text(description); $(".js-view-count-work_24148656").attr('title', description).tooltip(); }); });</script></span><script>$(function() { $(".js-view-count-work_24148656").removeClass('hidden') })</script></div></li><li class="InlineList-item u-positionRelative" style="max-width: 250px"><div class="u-positionAbsolute" data-has-card-for-ri-list="24148656"><i class="fa fa-tag InlineList-item-icon u-positionRelative"></i>  <a class="InlineList-item-text u-positionRelative">3</a>  </div><span class="InlineList-item-text u-textTruncate u-pl9x"><a class="InlineList-item-text" data-has-card-for-ri="28235" rel="nofollow" href="https://www.academia.edu/Documents/in/Multidisciplinary">Multidisciplinary</a>, <script data-card-contents-for-ri="28235" type="text/json">{"id":28235,"name":"Multidisciplinary","url":"https://www.academia.edu/Documents/in/Multidisciplinary","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="107664" rel="nofollow" href="https://www.academia.edu/Documents/in/Applied_Environmental_Microbiology">Applied Environmental Microbiology</a>, <script data-card-contents-for-ri="107664" type="text/json">{"id":107664,"name":"Applied Environmental Microbiology","url":"https://www.academia.edu/Documents/in/Applied_Environmental_Microbiology","nofollow":true}</script><a class="InlineList-item-text" data-has-card-for-ri="137610" rel="nofollow" href="https://www.academia.edu/Documents/in/Quorum_Sensing">Quorum Sensing</a><script data-card-contents-for-ri="137610" type="text/json">{"id":137610,"name":"Quorum Sensing","url":"https://www.academia.edu/Documents/in/Quorum_Sensing","nofollow":true}</script></span></li><script>(function(){ if (true) { new Aedu.ResearchInterestListCard({ el: $('*[data-has-card-for-ri-list=24148656]'), work: {"id":24148656,"title":"Quorum sensing mechanisms mediated by farnesol in Ophiostoma piceae : its effect on the secretion of sterol esterase","created_at":"2016-04-07T01:09:39.082-07:00","owner_id":46572140,"url":"https://www.academia.edu/24148656/Quorum_sensing_mechanisms_mediated_by_farnesol_in_Ophiostoma_piceae_its_effect_on_the_secretion_of_sterol_esterase","slug":"Quorum_sensing_mechanisms_mediated_by_farnesol_in_Ophiostoma_piceae_its_effect_on_the_secretion_of_sterol_esterase","dom_id":"work_24148656","summary":"Ophiostoma piceae CECT 20416 is a dimorphic wood-staining fungus able to produce 17 an extracellular sterol-esterase/lipase (OPE) with great biotechnological interest. 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FtsZ assembles with GTP into filaments that hydrolyze the nucleotide at the association interface between monomers... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13700633" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Cell division protein FtsZ is the organizer of the cytokinetic Z-ring in most bacteria and a target for new antibiotics. FtsZ assembles with GTP into filaments that hydrolyze the nucleotide at the association interface between monomers and then disassemble. We have replaced FtsZ's GTP with nonnucleotide synthetic inhibitors of bacterial division. We searched for these small molecules among compounds from the literature, from virtual screening (VS), and from our in-house synthetic library (UCM), employing a fluorescence anisotropy primary assay. From these screens we have identified the polyhydroxy aromatic compound UCM05 and its simplified analogue UCM44 that specifically bind to Bacillus subtilis FtsZ monomers with micromolar affinities and perturb normal assembly, as examined with light scattering, polymer sedimentation, and negative stain electron microscopy. On the other hand, these ligands induce the cooperative assembly of nucleotide-devoid archaeal FtsZ into distinct well-ordered polymers, different from GTP-induced filaments. These FtsZ inhibitors impair localization of FtsZ into the Z-ring and inhibit bacterial cell division. The chlorinated analogue UCM53 inhibits the growth of clinical isolates of antibioticresistant Staphylococcus aureus and Enterococcus faecalis. We suggest that these interfacial inhibitors recapitulate binding and some assembly-inducing effects of GTP but impair the correct structural dynamics of FtsZ filaments and thus inhibit bacterial division, possibly by binding to a small fraction of the FtsZ molecules in a bacterial cell, which opens a new approach to FtsZ-based antibacterial drug discovery.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13700633" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="824178a5ef9427a3e220a216122d4775" rel="nofollow" data-download="{"attachment_id":45038922,"asset_id":13700633,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45038922/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32835360" href="https://independent.academia.edu/IsabelBarasoain">Isabel Barasoain</a><script data-card-contents-for-user="32835360" type="text/json">{"id":32835360,"first_name":"Isabel","last_name":"Barasoain","domain_name":"independent","page_name":"IsabelBarasoain","display_name":"Isabel Barasoain","profile_url":"https://independent.academia.edu/IsabelBarasoain","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-13700633">+1</span><div class="hidden js-additional-users-13700633"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://csic.academia.edu/SoniaHuecas">Sonia Huecas</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-13700633'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-13700633').html(); 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FtsZ assembles with GTP into filaments that hydrolyze the nucleotide at the association interface between monomers and then disassemble. We have replaced FtsZ's GTP with nonnucleotide synthetic inhibitors of bacterial division. We searched for these small molecules among compounds from the literature, from virtual screening (VS), and from our in-house synthetic library (UCM), employing a fluorescence anisotropy primary assay. From these screens we have identified the polyhydroxy aromatic compound UCM05 and its simplified analogue UCM44 that specifically bind to Bacillus subtilis FtsZ monomers with micromolar affinities and perturb normal assembly, as examined with light scattering, polymer sedimentation, and negative stain electron microscopy. On the other hand, these ligands induce the cooperative assembly of nucleotide-devoid archaeal FtsZ into distinct well-ordered polymers, different from GTP-induced filaments. These FtsZ inhibitors impair localization of FtsZ into the Z-ring and inhibit bacterial cell division. The chlorinated analogue UCM53 inhibits the growth of clinical isolates of antibioticresistant Staphylococcus aureus and Enterococcus faecalis. We suggest that these interfacial inhibitors recapitulate binding and some assembly-inducing effects of GTP but impair the correct structural dynamics of FtsZ filaments and thus inhibit bacterial division, possibly by binding to a small fraction of the FtsZ molecules in a bacterial cell, which opens a new approach to FtsZ-based antibacterial drug discovery.","publication":"ACS Chemical Biology","publication_with_fallback":"ACS Chemical Biology","downloadable_attachments":[{"id":45038922,"asset_id":13700633,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/45038922/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/45038922/Synthetic_inhibitors_of_bacterial_cell_d20160424-5036-1l6vtqz-libre.pdf?1461522538=\u0026response-content-disposition=attachment%3B+filename%3DSynthetic_Inhibitors_of_Bacterial_Cell_D.pdf\u0026Expires=1740138717\u0026Signature=WQoYdLN6LU2Z81wj50gyyRY3VD7FY3sUEpB4cBxXX6nX~8-GfUlPpaF95RCX4S3qZ6bTlck3JP~gP4T5LZKc9W9nLmIKyxyYp~UNJgNnQTbIgLPLSH5Jyyy1utuytiisqlzmpfOpvjXqwsqu2LK8TeXuyD1lCq7HxPoGj~x0TZIe4~44rNmyrgQ~RYeByJQB~ezxXVGv5ReDruWxgkkdW-gfLzowoBMVwPpbSMg7TA-gbwEJ0T6I2XbZHnf8mrCfrxdRXnEB2voiQLlLZXs9sCg0ZwhgRiyZ~IS9GiLea0DVjo~GL-d9aav5UtaKA7N1bcbh5IZmQ8dTLnImcjamQg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/45038922/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/45038922/mini_magick20220703-28582-yygqol.png?1656881564"}],"downloadable_attachments_with_full_thumbnails":[{"id":45038922,"asset_id":13700633,"asset_type":"Work","always_allow_download":false,"scribd_thumbnail_url":"https://attachments.academia-assets.com/45038922/thumbnails/1.jpg","download_url":"https://d1wqtxts1xzle7.cloudfront.net/45038922/Synthetic_inhibitors_of_bacterial_cell_d20160424-5036-1l6vtqz-libre.pdf?1461522538=\u0026response-content-disposition=attachment%3B+filename%3DSynthetic_Inhibitors_of_Bacterial_Cell_D.pdf\u0026Expires=1740138717\u0026Signature=WQoYdLN6LU2Z81wj50gyyRY3VD7FY3sUEpB4cBxXX6nX~8-GfUlPpaF95RCX4S3qZ6bTlck3JP~gP4T5LZKc9W9nLmIKyxyYp~UNJgNnQTbIgLPLSH5Jyyy1utuytiisqlzmpfOpvjXqwsqu2LK8TeXuyD1lCq7HxPoGj~x0TZIe4~44rNmyrgQ~RYeByJQB~ezxXVGv5ReDruWxgkkdW-gfLzowoBMVwPpbSMg7TA-gbwEJ0T6I2XbZHnf8mrCfrxdRXnEB2voiQLlLZXs9sCg0ZwhgRiyZ~IS9GiLea0DVjo~GL-d9aav5UtaKA7N1bcbh5IZmQ8dTLnImcjamQg__\u0026Key-Pair-Id=APKAJLOHF5GGSLRBV4ZA","download_file_url":"https://www.academia.edu/attachments/45038922/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&","full_thumbnail_url":"https://0.academia-photos.com/attachment_thumbnails/45038922/mini_magick20220703-28582-yygqol.png?1656881564"}],"has_pdf":true,"has_fulltext":true,"page_count":12,"ordered_authors":[{"id":32835360,"first_name":"Isabel","last_name":"Barasoain","domain_name":"independent","page_name":"IsabelBarasoain","display_name":"Isabel Barasoain","profile_url":"https://independent.academia.edu/IsabelBarasoain","photo":"/images/s65_no_pic.png"},{"id":32923211,"first_name":"Sonia","last_name":"Huecas","domain_name":"csic","page_name":"SoniaHuecas","display_name":"Sonia Huecas","profile_url":"https://csic.academia.edu/SoniaHuecas","photo":"/images/s65_no_pic.png"}],"research_interests":[{"id":10640,"name":"Drug Discovery","url":"https://www.academia.edu/Documents/in/Drug_Discovery","nofollow":true},{"id":47884,"name":"Biological Sciences","url":"https://www.academia.edu/Documents/in/Biological_Sciences","nofollow":true},{"id":113903,"name":"Bacteria","url":"https://www.academia.edu/Documents/in/Bacteria","nofollow":true},{"id":260118,"name":"CHEMICAL SCIENCES","url":"https://www.academia.edu/Documents/in/CHEMICAL_SCIENCES","nofollow":true},{"id":335984,"name":"Anti-Bacterial Agents","url":"https://www.academia.edu/Documents/in/Anti-Bacterial_Agents"},{"id":386872,"name":"Bacillus subtilis","url":"https://www.academia.edu/Documents/in/Bacillus_subtilis"},{"id":418954,"name":"Guanosine Triphosphate","url":"https://www.academia.edu/Documents/in/Guanosine_Triphosphate"},{"id":587528,"name":"Bacterial infections","url":"https://www.academia.edu/Documents/in/Bacterial_infections"},{"id":1490349,"name":"Halogenation","url":"https://www.academia.edu/Documents/in/Halogenation"}],"publication_year":2013,"publication_year_with_fallback":2013,"paper_rank":null,"all_time_views":25,"active_discussion":{}}, }) } })();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13700646 coauthored" data-work_id="13700646" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13700646/Effective_GTP_Replacing_FtsZ_Inhibitors_and_Antibacterial_Mechanism_of_Action">Effective GTP-Replacing FtsZ Inhibitors and Antibacterial Mechanism of Action</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Essential cell division protein FtsZ is considered an attractive target in the search for antibacterials with novel mechanisms of action to overcome the resistance problem. FtsZ undergoes GTP-dependent assembly at midcell to form the... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13700646" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Essential cell division protein FtsZ is considered an attractive target in the search for antibacterials with novel mechanisms of action to overcome the resistance problem. FtsZ undergoes GTP-dependent assembly at midcell to form the Z-ring, a dynamic structure that evolves until final constriction of the cell. Therefore, molecules able to inhibit its activity will eventually disrupt bacterial viability. In this work, we report a new series of small molecules able to replace GTP and to specifically inhibit FtsZ, blocking the bacterial division process. These new synthesized inhibitors interact with the GTP-binding site of FtsZ (K d = 0.4−0.8 μM), display antibacterial activity against Gram-positive pathogenic bacteria, and show selectivity against tubulin. Biphenyl derivative 28 stands out as a potent FtsZ inhibitor (K d = 0.5 μM) with high antibacterial activity [MIC (MRSA) = 7 μM]. In-depth analysis of the mechanism of action of compounds 22, 28, 33, and 36 has revealed that they act as effective inhibitors of correct FtsZ assembly, blocking bacterial division and thus leading to filamentous undivided cells. These findings provide a compelling rationale for the development of compounds targeting the GTP-binding site as antibacterial agents and open the door to antibiotics with novel mechanisms of action.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13700646" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="b7003e70d30404644a2f47d7d06ac819" rel="nofollow" data-download="{"attachment_id":45038928,"asset_id":13700646,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/45038928/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32835360" href="https://independent.academia.edu/IsabelBarasoain">Isabel Barasoain</a><script data-card-contents-for-user="32835360" type="text/json">{"id":32835360,"first_name":"Isabel","last_name":"Barasoain","domain_name":"independent","page_name":"IsabelBarasoain","display_name":"Isabel Barasoain","profile_url":"https://independent.academia.edu/IsabelBarasoain","photo":"/images/s65_no_pic.png"}</script></span></span><span class="u-displayInlineBlock InlineList-item-text"> and <span class="u-textDecorationUnderline u-clickable InlineList-item-text js-work-more-authors-13700646">+1</span><div class="hidden js-additional-users-13700646"><div><span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a href="https://csic.academia.edu/SoniaHuecas">Sonia Huecas</a></span></div></div></span><script>(function(){ var popoverSettings = { el: $('.js-work-more-authors-13700646'), placement: 'bottom', hide_delay: 200, html: true, content: function(){ return $('.js-additional-users-13700646').html(); 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FtsZ undergoes GTP-dependent assembly at midcell to form the Z-ring, a dynamic structure that evolves until final constriction of the cell. Therefore, molecules able to inhibit its activity will eventually disrupt bacterial viability. In this work, we report a new series of small molecules able to replace GTP and to specifically inhibit FtsZ, blocking the bacterial division process. These new synthesized inhibitors interact with the GTP-binding site of FtsZ (K d = 0.4−0.8 μM), display antibacterial activity against Gram-positive pathogenic bacteria, and show selectivity against tubulin. Biphenyl derivative 28 stands out as a potent FtsZ inhibitor (K d = 0.5 μM) with high antibacterial activity [MIC (MRSA) = 7 μM]. In-depth analysis of the mechanism of action of compounds 22, 28, 33, and 36 has revealed that they act as effective inhibitors of correct FtsZ assembly, blocking bacterial division and thus leading to filamentous undivided cells. These findings provide a compelling rationale for the development of compounds targeting the GTP-binding site as antibacterial agents and open the door to antibiotics with novel mechanisms of action.","publication":"ACS Chemical Biology","publication_with_fallback":"ACS Chemical 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})();</script></ul></li></ul></div></div><div class="u-borderBottom1 u-borderColorGrayLighter"><div class="clearfix u-pv7x u-mb0x js-work-card work_13827914" data-work_id="13827914" itemscope="itemscope" itemtype="https://schema.org/ScholarlyArticle"><div class="header"><div class="title u-fontSerif u-fs22 u-lineHeight1_3"><a class="u-tcGrayDarkest js-work-link" href="https://www.academia.edu/13827914/Insights_into_Nucleotide_Recognition_by_Cell_Division_Protein_FtsZ_from_a_mant_GTP_Competition_Assay_and_Molecular_Dynamics">Insights into Nucleotide Recognition by Cell Division Protein FtsZ from a mant -GTP Competition Assay and Molecular Dynamics</a></div></div><div class="u-pb4x u-mt3x"><div class="summary u-fs14 u-fw300 u-lineHeight1_5 u-tcGrayDarkest"><div class="summarized">Essential cell division protein FtsZ forms the bacterial cytokinetic ring and is a target for new antibiotics. FtsZ monomers bind GTP and assemble into filaments. Hydrolysis to GDP at the association interface between monomers leads to... <a class="more_link u-tcGrayDark u-linkUnstyled" data-container=".work_13827914" data-show=".complete" data-hide=".summarized" data-more-link-behavior="true" href="#">more</a></div><div class="complete hidden">Essential cell division protein FtsZ forms the bacterial cytokinetic ring and is a target for new antibiotics. FtsZ monomers bind GTP and assemble into filaments. Hydrolysis to GDP at the association interface between monomers leads to filament disassembly. We have developed a homogeneous competition assay, employing the fluorescence anisotropy change of mant-GTP upon binding to nucleotide-free FtsZ, which detects compounds binding to the nucleotide site in FtsZ monomers and measures their affinities within the millimolar to 10 nM range. We have employed this method to determine the apparent contributions of the guanine, ribose, and the R-, β-, and γ-phosphates to the free energy change of nucleotide binding. Similar relative contributions have also been estimated through molecular dynamics and binding free energy calculations, employing the crystal structures of FtsZ-nucleotide complexes. We find an energetically dominant contribution of the β-phosphate, comparable to the whole guanosine moiety. GTP and GDP bind with similar observed affinity to FtsZ monomers. Loss of the regulatory γ-phosphate results in a predicted accommodation of GDP which has not been observed in the crystal structures. The binding affinities of a series of C8-substituted GTP analogues, known to inhibit FtsZ but not eukaryotic tubulin assembly, correlate with their inhibitory capacity on FtsZ polymerization. Our methods permit testing of FtsZ inhibitors targeting its nucleotide site, as well as compounds from virtual screening of large synthetic libraries. Our results give insight into the FtsZ-nucleotide interactions, which could be useful in the rational design of new inhibitors, especially GTP phosphate mimetics.</div></div></div><ul class="InlineList u-ph0x u-fs13"><li class="InlineList-item logged_in_only"><div class="share_on_academia_work_button"><a class="academia_share Button Button--inverseBlue Button--sm js-bookmark-button" data-academia-share="Work/13827914" data-share-source="work_strip" data-spinner="small_white_hide_contents"><i class="fa fa-plus"></i><span class="work-strip-link-text u-ml1x" data-content="button_text">Bookmark</span></a></div></li><li class="InlineList-item"><div class="download"><a id="542e1c91264c47675172f21f5135e2c3" rel="nofollow" data-download="{"attachment_id":44905042,"asset_id":13827914,"asset_type":"Work","always_allow_download":false,"track":null,"button_location":"work_strip","source":null,"hide_modal":null}" class="Button Button--sm Button--inverseGreen js-download-button prompt_button doc_download" href="https://www.academia.edu/attachments/44905042/download_file?st=MTc0MDE1NDA2MSw4LjIyMi4yMDguMTQ2&s=work_strip"><i class="fa fa-arrow-circle-o-down fa-lg"></i><span class="u-textUppercase u-ml1x" data-content="button_text">Download</span></a></div></li><li class="InlineList-item"><ul class="InlineList InlineList--bordered u-ph0x"><li class="InlineList-item InlineList-item--bordered"><span class="InlineList-item-text">by <span itemscope="itemscope" itemprop="author" itemtype="https://schema.org/Person"><a class="u-tcGrayDark u-fw700" data-has-card-for-user="32923211" href="https://csic.academia.edu/SoniaHuecas">Sonia Huecas</a><script data-card-contents-for-user="32923211" type="text/json">{"id":32923211,"first_name":"Sonia","last_name":"Huecas","domain_name":"csic","page_name":"SoniaHuecas","display_name":"Sonia Huecas","profile_url":"https://csic.academia.edu/SoniaHuecas","photo":"/images/s65_no_pic.png"}</script></span></span></li><li class="js-paper-rank-work_13827914 InlineList-item InlineList-item--bordered hidden"><span class="js-paper-rank-view hidden u-tcGrayDark" data-paper-rank-work-id="13827914"><i class="u-m1x fa fa-bar-chart"></i><strong class="js-paper-rank"></strong></span><script>$(function() { new Works.PaperRankView({ workId: 13827914, container: ".js-paper-rank-work_13827914", }); 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FtsZ monomers bind GTP and assemble into filaments. Hydrolysis to GDP at the association interface between monomers leads to filament disassembly. We have developed a homogeneous competition assay, employing the fluorescence anisotropy change of mant-GTP upon binding to nucleotide-free FtsZ, which detects compounds binding to the nucleotide site in FtsZ monomers and measures their affinities within the millimolar to 10 nM range. We have employed this method to determine the apparent contributions of the guanine, ribose, and the R-, β-, and γ-phosphates to the free energy change of nucleotide binding. Similar relative contributions have also been estimated through molecular dynamics and binding free energy calculations, employing the crystal structures of FtsZ-nucleotide complexes. We find an energetically dominant contribution of the β-phosphate, comparable to the whole guanosine moiety. GTP and GDP bind with similar observed affinity to FtsZ monomers. Loss of the regulatory γ-phosphate results in a predicted accommodation of GDP which has not been observed in the crystal structures. The binding affinities of a series of C8-substituted GTP analogues, known to inhibit FtsZ but not eukaryotic tubulin assembly, correlate with their inhibitory capacity on FtsZ polymerization. Our methods permit testing of FtsZ inhibitors targeting its nucleotide site, as well as compounds from virtual screening of large synthetic libraries. 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