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principles</title> <meta name="description" content="Search results for: editing principles"> <meta name="keywords" content="editing principles"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="editing principles"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 2078</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: editing principles</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2078</span> Principles of Editing and Storytelling in Relation to Editorial Graphic Design </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Melike%20Tascioglu">Melike Tascioglu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper aims to combine film editing principles to basic design principles to explore what graphic designers do in terms of storytelling. The sequential aspect of film is designed and examined through the art of editing. Examining the rules, principles and formulas of film editing can be a method for graphic designers to further practice the art of storytelling. Although there are many research and publications on design basics, time, pace, dramatic structure and choreography are not very well defined in the area of graphic design. In this era of creative storytelling and interdisciplinary collaboration, not only film editors but also graphic designers and students in the arts and design should understand the theory and practice of editing to be able to create a strong mise-en-scène and not only a mise-en-page. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=design%20principles" title="design principles">design principles</a>, <a href="https://publications.waset.org/abstracts/search?q=editing%20principles" title=" editing principles"> editing principles</a>, <a href="https://publications.waset.org/abstracts/search?q=editorial%20design" title=" editorial design"> editorial design</a>, <a href="https://publications.waset.org/abstracts/search?q=film%20editing" title=" film editing"> film editing</a>, <a href="https://publications.waset.org/abstracts/search?q=graphic%20design" title=" graphic design"> graphic design</a>, <a href="https://publications.waset.org/abstracts/search?q=storytelling" title=" storytelling"> storytelling</a> </p> <a href="https://publications.waset.org/abstracts/3528/principles-of-editing-and-storytelling-in-relation-to-editorial-graphic-design" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/3528.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">330</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2077</span> Genome Editing in Sorghum: Advancements and Future Possibilities: A Review</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Micheale%20Yifter%20Weldemichael">Micheale Yifter Weldemichael</a>, <a href="https://publications.waset.org/abstracts/search?q=Hailay%20Mehari%20Gebremedhn"> Hailay Mehari Gebremedhn</a>, <a href="https://publications.waset.org/abstracts/search?q=Teklehaimanot%20Hailesslasie"> Teklehaimanot Hailesslasie</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The advancement of target-specific genome editing tools, including clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein9 (Cas9), mega-nucleases, base editing (BE), prime editing (PE), transcription activator-like endonucleases (TALENs), and zinc-finger nucleases (ZFNs), have paved the way for a modern era of gene editing. CRISPR/Cas9, as a versatile, simple, cost-effective and robust system for genome editing, has dominated the genome manipulation field over the last few years. The application of CRISPR/Cas9 in sorghum improvement is particularly vital in the context of ecological, environmental and agricultural challenges, as well as global climate change. In this context, gene editing using CRISPR/Cas9 can improve nutritional value, yield, resistance to pests and disease and tolerance to different abiotic stress. Moreover, CRISPR/Cas9 can potentially perform complex editing to reshape already available elite varieties and new genetic variations. However, existing research is targeted at improving even further the effectiveness of the CRISPR/Cas9 genome editing techniques to fruitfully edit endogenous sorghum genes. These findings suggest that genome editing is a feasible and successful venture in sorghum. Newer improvements and developments of CRISPR/Cas9 techniques have further qualified researchers to modify extra genes in sorghum with improved efficiency. The fruitful application and development of CRISPR techniques for genome editing in sorghum will not only help in gene discovery, creating new, improved traits in sorghum regulating gene expression sorghum functional genomics, but also in making site-specific integration events. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR%2FCas9" title="CRISPR/Cas9">CRISPR/Cas9</a>, <a href="https://publications.waset.org/abstracts/search?q=genome%20editing" title=" genome editing"> genome editing</a>, <a href="https://publications.waset.org/abstracts/search?q=quality" title=" quality"> quality</a>, <a href="https://publications.waset.org/abstracts/search?q=sorghum" title=" sorghum"> sorghum</a>, <a href="https://publications.waset.org/abstracts/search?q=stress" title=" stress"> stress</a>, <a href="https://publications.waset.org/abstracts/search?q=yield" title=" yield"> yield</a> </p> <a href="https://publications.waset.org/abstracts/177564/genome-editing-in-sorghum-advancements-and-future-possibilities-a-review" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/177564.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">59</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2076</span> Design and Implementation of Collaborative Editing System Based on Physical Simulation Engine Running State</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zhang%20Songning">Zhang Songning</a>, <a href="https://publications.waset.org/abstracts/search?q=Guan%20Zheng"> Guan Zheng</a>, <a href="https://publications.waset.org/abstracts/search?q=Ci%20Yan"> Ci Yan</a>, <a href="https://publications.waset.org/abstracts/search?q=Ding%20Gangyi"> Ding Gangyi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The application of physical simulation engines in collaborative editing systems has an important background and role. Firstly, physical simulation engines can provide real-world physical simulations, enabling users to interact and collaborate in real time in virtual environments. This provides a more intuitive and immersive experience for collaborative editing systems, allowing users to more accurately perceive and understand various elements and operations in collaborative editing. Secondly, through physical simulation engines, different users can share virtual space and perform real-time collaborative editing within it. This real-time sharing and collaborative editing method helps to synchronize information among team members and improve the efficiency of collaborative work. Through experiments, the average model transmission speed of a single person in the collaborative editing system has increased by 141.91%; the average model processing speed of a single person has increased by 134.2%; the average processing flow rate of a single person has increased by 175.19%; the overall efficiency improvement rate of a single person has increased by 150.43%. With the increase in the number of users, the overall efficiency remains stable, and the physical simulation engine running status collaborative editing system also has horizontal scalability. It is not difficult to see that the design and implementation of a collaborative editing system based on physical simulation engines not only enriches the user experience but also optimizes the effectiveness of team collaboration, providing new possibilities for collaborative work. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=physics%20engine" title="physics engine">physics engine</a>, <a href="https://publications.waset.org/abstracts/search?q=simulation%20technology" title=" simulation technology"> simulation technology</a>, <a href="https://publications.waset.org/abstracts/search?q=collaborative%20editing" title=" collaborative editing"> collaborative editing</a>, <a href="https://publications.waset.org/abstracts/search?q=system%20design" title=" system design"> system design</a>, <a href="https://publications.waset.org/abstracts/search?q=data%20transmission" title=" data transmission"> data transmission</a> </p> <a href="https://publications.waset.org/abstracts/178976/design-and-implementation-of-collaborative-editing-system-based-on-physical-simulation-engine-running-state" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/178976.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">85</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2075</span> Photoleap: An AI-Powered Photo Editing App with Advanced Features and User Satisfaction Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Joud%20Basyouni">Joud Basyouni</a>, <a href="https://publications.waset.org/abstracts/search?q=Rama%20Zagzoog"> Rama Zagzoog</a>, <a href="https://publications.waset.org/abstracts/search?q=Mashael%20Al%20Faleh"> Mashael Al Faleh</a>, <a href="https://publications.waset.org/abstracts/search?q=Jana%20Alireza"> Jana Alireza</a> </p> <p class="card-text"><strong>Abstract:</strong></p> AI is changing many fields and speeding up tasks that used to take a long time. It used to take too long to edit photos. However, many AI-powered apps make photo editing, automatic effects, and animations much easier than other manual editing apps with no AI. The mobile app Photoleap edits photos and creates digital art using AI. Editing photos with text prompts is also becoming a standard these days with the help of apps like Photoleap. Now, users can change backgrounds, add animations, turn text into images, and create scenes with AI. This project report discusses the photo editing app's history and popularity. Photoleap resembles Photoshop, Canva, Photos, and Pixlr. The report includes survey questions to assess Photoleap user satisfaction. The report describes Photoleap's features and functions with screenshots. Photoleap uses AI well. Charts and graphs show Photoleap user ratings and comments from the survey. This project found that most Photoleap users liked how well it worked, was made, and was easy to use. People liked changing photos and adding backgrounds. Users can create stunning photo animations. A few users dislike the app's animations, AI art, and photo effects. The project report discusses the app's pros and cons and offers improvements. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=artificial%20intelligence" title="artificial intelligence">artificial intelligence</a>, <a href="https://publications.waset.org/abstracts/search?q=photoleap" title=" photoleap"> photoleap</a>, <a href="https://publications.waset.org/abstracts/search?q=images" title=" images"> images</a>, <a href="https://publications.waset.org/abstracts/search?q=background" title=" background"> background</a>, <a href="https://publications.waset.org/abstracts/search?q=photo%20editing" title=" photo editing"> photo editing</a> </p> <a href="https://publications.waset.org/abstracts/179287/photoleap-an-ai-powered-photo-editing-app-with-advanced-features-and-user-satisfaction-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/179287.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">60</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2074</span> Ethical Considerations in In-Utero Gene Editing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Shruti%20Govindarajan">Shruti Govindarajan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In-utero gene editing with CRISPR-Cas9 opens up new possibilities for treating genetic disorders during pregnancy while still in mother’s womb. By targeting genetic mutations in the early stages of fetal development, this approach could potentially prevent severe conditions—like cystic fibrosis, sickle cell anemia, and muscular dystrophy—from causing harm. CRISPR-Cas9, which allows precise DNA edits, could be delivered into fetal cells through vectors such as adeno-associated viruses (AAVs) or nanoparticles, correcting disease-causing mutations and possibly offering lifelong relief from these disorders. For families facing severe genetic diagnoses, in-utero gene editing could provide a transformative option. However, technical challenges remain, including ensuring that gene editing only targets the intended cells and verifying long-term safety. Ethical considerations are also at the forefront of this technology. The editing of a fetus's genes brings up difficult questions about consent, especially since these genetic changes will affect the child’s entire life without their input. There's also concern over possible unintended side effects, or changes passed down to future generations. Moreover, if used beyond therapeutic purposes, this technology could be misused for ‘enhancements,’ like selecting for certain physical or cognitive traits, raising concerns about inequality and social pressures. In this way, in-utero gene editing brings both exciting potential and complex moral questions. As research progresses, addressing these scientific and ethical concerns will be key to ensuring that this technology is used responsibly, prioritizing safety, fairness, and a focus on alleviating genetic disease. A cautious and inclusive approach, along with clear regulations, will be essential to realizing the benefits of in-utero gene editing while protecting against unintended consequences. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=in-utero%20gene%20editing" title="in-utero gene editing">in-utero gene editing</a>, <a href="https://publications.waset.org/abstracts/search?q=CRISPR" title=" CRISPR"> CRISPR</a>, <a href="https://publications.waset.org/abstracts/search?q=bioethics" title=" bioethics"> bioethics</a>, <a href="https://publications.waset.org/abstracts/search?q=genetic%20disorder" title=" genetic disorder"> genetic disorder</a> </p> <a href="https://publications.waset.org/abstracts/194663/ethical-considerations-in-in-utero-gene-editing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/194663.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">7</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2073</span> Intelligent CRISPR Design for Bone Regeneration</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yu-Chen%20Hu">Yu-Chen Hu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Gene editing by CRISPR and gene regulation by microRNA or CRISPR activation have dramatically changed the way to manipulate cellular gene expression and cell fate. In recent years, various gene editing and gene manipulation technologies have been applied to control stem cell differentiation to enhance tissue regeneration. This research will focus on how to develop CRISPR, CRISPR activation (CRISPRa), CRISPR inhibition (CRISPRi), as well as bi-directional CRISPR-AI gene regulation technologies to control cell differentiation and bone regeneration. Moreover, in this study, CRISPR/Cas13d-mediated RNA editng for miRNA editing and bone regeneration will be discussed. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=gene%20therapy" title="gene therapy">gene therapy</a>, <a href="https://publications.waset.org/abstracts/search?q=bone%20regeneration" title=" bone regeneration"> bone regeneration</a>, <a href="https://publications.waset.org/abstracts/search?q=stem%20cell" title=" stem cell"> stem cell</a>, <a href="https://publications.waset.org/abstracts/search?q=CRISPR" title=" CRISPR"> CRISPR</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20regulation" title=" gene regulation"> gene regulation</a> </p> <a href="https://publications.waset.org/abstracts/168750/intelligent-crispr-design-for-bone-regeneration" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/168750.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">90</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2072</span> Optimizing the Use of Google Translate in Translation Teaching: A Case Study at Prince Sultan University </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saadia%20Elamin">Saadia Elamin</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The quasi-universal use of smart phones with internet connection available all the time makes it a reflex action for translation undergraduates, once they encounter the least translation problem, to turn to the freely available web resource: Google Translate. Like for other translator resources and aids, the use of Google Translate needs to be moderated in such a way that it contributes to developing translation competence. Here, instead of interfering with students’ learning by providing ready-made solutions which might not always fit into the contexts of use, it can help to consolidate the skills of analysis and transfer which students have already acquired. One way to do so is by training students to adhere to the basic principles of translation work. The most important of these is that analyzing the source text for comprehension comes first and foremost before jumping into the search for target language equivalents. Another basic principle is that certain translator aids and tools can be used for comprehension, while others are to be confined to the phase of re-expressing the meaning into the target language. The present paper reports on the experience of making a measured and reasonable use of Google Translate in translation teaching at Prince Sultan University (PSU), Riyadh. First, it traces the development that has taken place in the field of translation in this age of information technology, be it in translation teaching and translator training, or in the real-world practice of the profession. Second, it describes how, with the aim of reflecting this development onto the way translation is taught, senior students, after being trained on post-editing machine translation output, are authorized to use Google Translate in classwork and assignments. Third, the paper elaborates on the findings of this case study which has demonstrated that Google Translate, if used at the appropriate levels of training, can help to enhance students’ ability to perform different translation tasks. This help extends from the search for terms and expressions, to the tasks of drafting the target text, revising its content and finally editing it. In addition, using Google Translate in this way fosters a reflexive and critical attitude towards web resources in general, maximizing thus the benefit gained from them in preparing students to meet the requirements of the modern translation job market. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Google%20Translate" title="Google Translate">Google Translate</a>, <a href="https://publications.waset.org/abstracts/search?q=post-editing%20machine%20translation%20output" title=" post-editing machine translation output"> post-editing machine translation output</a>, <a href="https://publications.waset.org/abstracts/search?q=principles%20of%20translation%20work" title=" principles of translation work"> principles of translation work</a>, <a href="https://publications.waset.org/abstracts/search?q=translation%20competence" title=" translation competence"> translation competence</a>, <a href="https://publications.waset.org/abstracts/search?q=translation%20teaching" title=" translation teaching"> translation teaching</a>, <a href="https://publications.waset.org/abstracts/search?q=translator%20aids%20and%20tools" title=" translator aids and tools"> translator aids and tools</a> </p> <a href="https://publications.waset.org/abstracts/60413/optimizing-the-use-of-google-translate-in-translation-teaching-a-case-study-at-prince-sultan-university" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/60413.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">473</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2071</span> CRISPR-DT: Designing gRNAs for the CRISPR-Cpf1 System with Improved Target Efficiency and Specificity</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Houxiang%20Zhu">Houxiang Zhu</a>, <a href="https://publications.waset.org/abstracts/search?q=Chun%20Liang"> Chun Liang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The CRISPR-Cpf1 system has been successfully applied in genome editing. However, target efficiency of the CRISPR-Cpf1 system varies among different gRNA sequences. The published CRISPR-Cpf1 gRNA data was reanalyzed. Many sequences and structural features of gRNAs (e.g., the position-specific nucleotide composition, position-nonspecific nucleotide composition, GC content, minimum free energy, and melting temperature) correlated with target efficiency were found. Using machine learning technology, a support vector machine (SVM) model was created to predict target efficiency for any given gRNAs. The first web service application, CRISPR-DT (CRISPR DNA Targeting), has been developed to help users design optimal gRNAs for the CRISPR-Cpf1 system by considering both target efficiency and specificity. CRISPR-DT will empower researchers in genome editing. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR-Cpf1" title="CRISPR-Cpf1">CRISPR-Cpf1</a>, <a href="https://publications.waset.org/abstracts/search?q=genome%20editing" title=" genome editing"> genome editing</a>, <a href="https://publications.waset.org/abstracts/search?q=target%20efficiency" title=" target efficiency"> target efficiency</a>, <a href="https://publications.waset.org/abstracts/search?q=target%20specificity" title=" target specificity"> target specificity</a> </p> <a href="https://publications.waset.org/abstracts/93235/crispr-dt-designing-grnas-for-the-crispr-cpf1-system-with-improved-target-efficiency-and-specificity" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/93235.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">262</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2070</span> Precise Identification of Clustered Regularly Interspaced Short Palindromic Repeats-Induced Mutations via Hidden Markov Model-Based Sequence Alignment</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jingyuan%20Hu">Jingyuan Hu</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhandong%20Liu"> Zhandong Liu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> CRISPR genome editing technology has transformed molecular biology by accurately targeting and altering an organism’s DNA. Despite the state-of-art precision of CRISPR genome editing, the imprecise mutation outcome and off-target effects present considerable risk, potentially leading to unintended genetic changes. Targeted deep sequencing, combined with bioinformatics sequence alignment, can detect such unwanted mutations. Nevertheless, the classical method, Needleman-Wunsch (NW) algorithm may produce false alignment outcomes, resulting in inaccurate mutation identification. The key to precisely identifying CRISPR-induced mutations lies in determining optimal parameters for the sequence alignment algorithm. Hidden Markov models (HMM) are ideally suited for this task, offering flexibility across CRISPR systems by leveraging forward-backward algorithms for parameter estimation. In this study, we introduce CRISPR-HMM, a statistical software to precisely call CRISPR-induced mutations. We demonstrate that the software significantly improves precision in identifying CRISPR-induced mutations compared to NW-based alignment, thereby enhancing the overall understanding of the CRISPR gene-editing process. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR" title="CRISPR">CRISPR</a>, <a href="https://publications.waset.org/abstracts/search?q=HMM" title=" HMM"> HMM</a>, <a href="https://publications.waset.org/abstracts/search?q=sequence%20alignment" title=" sequence alignment"> sequence alignment</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20editing" title=" gene editing"> gene editing</a> </p> <a href="https://publications.waset.org/abstracts/183505/precise-identification-of-clustered-regularly-interspaced-short-palindromic-repeats-induced-mutations-via-hidden-markov-model-based-sequence-alignment" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/183505.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">51</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2069</span> Altered Expression of Ubiquitin Editing Complex in Ulcerative Colitis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ishani%20Majumdar">Ishani Majumdar</a>, <a href="https://publications.waset.org/abstracts/search?q=Jaishree%20Paul"> Jaishree Paul</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Introduction: Ulcerative Colitis (UC) is an inflammatory disease of the colon resulting from an autoimmune response towards individual’s own microbiota. Excessive inflammation is characterized by hyper-activation of NFkB, a transcription factor regulating expression of various pro-inflammatory genes. The ubiquitin editing complex consisting of TNFAIP3, ITCH, RNF11 and TAX1BP1 maintains homeostatic levels of active NFkB through feedback inhibition and assembles in response to various stimuli that activate NFkB. TNFAIP3 deubiquitinates key signaling molecules involved in NFkB activation pathway. ITCH, RNF11 and TAX1BP1 provide substrate specificity, acting as adaptors for TNFAIP3 function. Aim: This study aimed to find expression of members of the ubiquitin editing complex at the transcript level in inflamed colon tissues of UC patients. Materials and Methods: Colonic biopsy samples were collected from 30 UC patients recruited at Department of Gastroenterology, AIIMS (New Delhi). Control group (n= 10) consisted of individuals undergoing examination for functional disorders. Real Time PCR was used to determine relative expression with GAPDH as housekeeping gene. Results: Expression of members of the ubiquitin editing complex was significantly altered during active disease. Expression of TNFAIP3 was upregulated while concomitant decrease in expression of ITCH, RNF11, TAX1BP1 was seen in UC patients. Discussion: This study reveals that increase in expression of TNFAIP3 was unable to control inflammation during active UC. Further, insufficient upregulation of ITCH, RNF11, TAX1BP1 may limit the formation of the ubiquitin complex and contribute to pathogenesis of UC. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=altered%20expression" title="altered expression">altered expression</a>, <a href="https://publications.waset.org/abstracts/search?q=inflammation" title=" inflammation"> inflammation</a>, <a href="https://publications.waset.org/abstracts/search?q=ubiquitin%20editing%20complex" title=" ubiquitin editing complex"> ubiquitin editing complex</a>, <a href="https://publications.waset.org/abstracts/search?q=ulcerative%20colitis" title=" ulcerative colitis"> ulcerative colitis</a> </p> <a href="https://publications.waset.org/abstracts/45510/altered-expression-of-ubiquitin-editing-complex-in-ulcerative-colitis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/45510.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">262</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2068</span> CRISPR-Mediated Genome Editing for Yield Enhancement in Tomato</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aswini%20M.%20S.">Aswini M. S.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Tomato (Solanum lycopersicum L.) is one of the most significant vegetable crops in terms of its economic benefits. Both fresh and processed tomatoes are consumed. Tomatoes have a limited genetic base, which makes breeding extremely challenging. Plant breeding has become much simpler and more effective with genome editing tools of CRISPR and CRISPR-associated 9 protein (CRISPR/Cas9), which address the problems with traditional breeding, chemical/physical mutagenesis, and transgenics. With the use of CRISPR/Cas9, a number of tomato traits have been functionally distinguished and edited. These traits include plant architecture as well as flower characters (leaf, flower, male sterility, and parthenocarpy), fruit ripening, quality and nutrition (lycopene, carotenoid, GABA, TSS, and shelf-life), disease resistance (late blight, TYLCV, and powdery mildew), tolerance to abiotic stress (heat, drought, and salinity) and resistance to herbicides. This study explores the potential of CRISPR/Cas9 genome editing for enhancing yield in tomato plants. The study utilized the CRISPR/Cas9 genome editing technology to functionally edit various traits in tomatoes. The de novo domestication of elite features from wild cousins to cultivated tomatoes and vice versa has been demonstrated by the introgression of CRISPR/Cas9. The CycB (Lycopene beta someri) gene-mediated Cas9 editing increased the lycopene content in tomato. Also, Cas9-mediated editing of the AGL6 (Agamous-like 6) gene resulted in parthenocarpic fruit development under heat-stress conditions. The advent of CRISPR/Cas has rendered it possible to use digital resources for single guide RNA design and multiplexing, cloning (such as Golden Gate cloning, GoldenBraid, etc.), creating robust CRISPR/Cas constructs, and implementing effective transformation protocols like the Agrobacterium and DNA free protoplast method for Cas9-gRNAs ribonucleoproteins (RNPs) complex. Additionally, homologous recombination (HR)-based gene knock-in (HKI) via geminivirus replicon and base/prime editing (Target-AID technology) remains possible. Hence, CRISPR/Cas facilitates fast and efficient breeding in the improvement of tomatoes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR-Cas" title="CRISPR-Cas">CRISPR-Cas</a>, <a href="https://publications.waset.org/abstracts/search?q=biotic%20and%20abiotic%20stress" title=" biotic and abiotic stress"> biotic and abiotic stress</a>, <a href="https://publications.waset.org/abstracts/search?q=flower%20and%20fruit%20traits" title=" flower and fruit traits"> flower and fruit traits</a>, <a href="https://publications.waset.org/abstracts/search?q=genome%20editing" title=" genome editing"> genome editing</a>, <a href="https://publications.waset.org/abstracts/search?q=polygenic%20trait" title=" polygenic trait"> polygenic trait</a>, <a href="https://publications.waset.org/abstracts/search?q=tomato%20and%20trait%20introgression" title=" tomato and trait introgression"> tomato and trait introgression</a> </p> <a href="https://publications.waset.org/abstracts/176028/crispr-mediated-genome-editing-for-yield-enhancement-in-tomato" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/176028.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">70</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2067</span> Advances in Genome Editing and Future Prospects for Sorghum Improvement: A Review</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Micheale%20Yifter%20Weldemichael">Micheale Yifter Weldemichael</a>, <a href="https://publications.waset.org/abstracts/search?q=Hailay%20Mehari%20Gebremedhn"> Hailay Mehari Gebremedhn</a>, <a href="https://publications.waset.org/abstracts/search?q=Teklehaimanot%20Hailesslasie%20Teklu"> Teklehaimanot Hailesslasie Teklu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Recent developments in targeted genome editing accelerated genetic research and opened new potentials to improve crops for better yields and quality. Given the significance of cereal crops as a primary source of food for the global population, the utilization of contemporary genome editing techniques like CRISPR/Cas9 is timely and crucial. CRISPR/Cas technology has enabled targeted genomic modifications, revolutionizing genetic research and exploration. Application of gene editing through CRISPR/Cas9 in enhancing sorghum is particularly vital given the current ecological, environmental, and agricultural challenges exacerbated by climate change. As sorghum is one of the main staple foods of our region and is known to be a resilient crop with a high potential to overcome the above challenges, the application of genome editing technology will enhance the investigation of gene functionality. CRISPR/Cas9 enables the improvement of desirable sorghum traits, including nutritional value, yield, resistance to pests and diseases, and tolerance to various abiotic stresses. Furthermore, CRISPR/Cas9 has the potential to perform intricate editing and reshape the existing elite sorghum varieties, and introduce new genetic variations. However, current research primarily focuses on improving the efficacy of the CRISPR/Cas9 system in successfully editing endogenous sorghum genes, making it a feasible and successful undertaking in sorghum improvement. Recent advancements and developments in CRISPR/Cas9 techniques have further empowered researchers to modify additional genes in sorghum with greater efficiency. Successful application and advancement of CRISPR techniques in sorghum will aid not only in gene discovery and the creation of novel traits that regulate gene expression and functional genomics but also in facilitating site-specific integration events. The purpose of this review is, therefore, to elucidate the current advances in sorghum genome editing and highlight its potential in addressing food security issues. It also assesses the efficiency of CRISPR-mediated improvement and its long-term effects on crop improvement and host resistance against parasites, including tissue-specific activity and the ability to induce resistance. This review ends by emphasizing the challenges and opportunities of CRISPR technology in combating parasitic plants and proposing directions for future research to safeguard global agricultural productivity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR%2FCas9" title="CRISPR/Cas9">CRISPR/Cas9</a>, <a href="https://publications.waset.org/abstracts/search?q=genome%20editing" title=" genome editing"> genome editing</a>, <a href="https://publications.waset.org/abstracts/search?q=quality" title=" quality"> quality</a>, <a href="https://publications.waset.org/abstracts/search?q=sorghum" title=" sorghum"> sorghum</a>, <a href="https://publications.waset.org/abstracts/search?q=stress" title=" stress"> stress</a>, <a href="https://publications.waset.org/abstracts/search?q=yield" title=" yield"> yield</a> </p> <a href="https://publications.waset.org/abstracts/188537/advances-in-genome-editing-and-future-prospects-for-sorghum-improvement-a-review" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/188537.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">38</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2066</span> Genetics, Law and Society: Regulating New Genetic Technologies</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aisling%20De%20Paor">Aisling De Paor</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Scientific and technological developments are driving genetics and genetic technologies into the public sphere. Scientists are making genetic discoveries as to the make up of the human body and the cause and effect of disease, diversity and disability amongst individuals. Technological innovation in the field of genetics is also advancing, with the development of genetic testing, and other emerging genetic technologies, including gene editing (which offers the potential for genetic modification). In addition to the benefits for medicine, health care and humanity, these genetic advances raise a range of ethical, legal and societal concerns. From an ethical perspective, such advances may, for example, change the concept of humans and what it means to be human. Science may take over in conceptualising human beings, which may push the boundaries of existing human rights. New genetic technologies, particularly gene editing techniques create the potential to stigmatise disability, by highlighting disability or genetic difference as something that should be eliminated or anticipated. From a disability perspective, use (and misuse) of genetic technologies raise concerns about discrimination and violations to the dignity and integrity of the individual. With an acknowledgement of the likely future orientation of genetic science, and in consideration of the intersection of genetics and disability, this paper highlights the main concerns raised as genetic science and technology advances (particularly with gene editing developments), and the consequences for disability and human rights. Through the use of traditional doctrinal legal methodologies, it investigates the use (and potential misuse) of gene editing as creating the potential for a unique form of discrimination and stigmatization to develop, as well as a potential gateway to a form of new, subtle eugenics. This article highlights the need to maintain caution as to the use, application and the consequences of genetic technologies. With a focus on the law and policy position in Europe, it examines the need to control and regulate these new technologies, particularly gene editing. In addition to considering the need for regulation, this paper highlights non-normative approaches to address this area, including awareness raising and education, public discussion and engagement with key stakeholders in the field and the development of a multifaceted genetics advisory network. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=disability" title="disability">disability</a>, <a href="https://publications.waset.org/abstracts/search?q=gene-editing" title=" gene-editing"> gene-editing</a>, <a href="https://publications.waset.org/abstracts/search?q=genetics" title=" genetics"> genetics</a>, <a href="https://publications.waset.org/abstracts/search?q=law" title=" law"> law</a>, <a href="https://publications.waset.org/abstracts/search?q=regulation" title=" regulation"> regulation</a> </p> <a href="https://publications.waset.org/abstracts/66469/genetics-law-and-society-regulating-new-genetic-technologies" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/66469.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">360</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2065</span> The Need for a Consistent Regulatory Framework for CRISPR Gene-Editing in the European Union</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Andrew%20Thayer">Andrew Thayer</a>, <a href="https://publications.waset.org/abstracts/search?q=Courtney%20Rondeau"> Courtney Rondeau</a>, <a href="https://publications.waset.org/abstracts/search?q=Paraskevi%20Papadopoulou"> Paraskevi Papadopoulou</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) gene-editing technologies have generated considerable discussion about the applications and ethics of their use. However, no consistent guidelines for using CRISPR technologies have been developed -nor common legislation passed related to gene editing, especially as it is connected to genetically modified organisms (GMOs) in the European Union. The recent announcement that the first babies with CRISPR-edited genes were born, along with new studies exploring CRISPR’s applications in treating thalassemia, sickle-cell anemia, cancer, and certain forms of blindness, have demonstrated that the technology is developing faster than the policies needed to control it. Therefore, it can be seen that a reasonable and coherent regulatory framework for the use of CRISPR in human somatic and germline cells is necessary to ensure the ethical use of the technology in future years. The European Union serves as a unique region of interconnected countries without a standard set of regulations or legislation for CRISPR gene-editing. We posit that the EU would serve as a suitable model in comparing the legislations of its affiliated countries in order to understand the practicality and effectiveness of adopting majority-approved practices. Additionally, we present a proposed set of guidelines which could serve as a basis in developing a consistent regulatory framework for the EU countries to implement but also act as a good example for other countries to adhere to. Finally, an additional, multidimensional framework of smart solutions is proposed with which all stakeholders are engaged to become better-informed citizens. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR" title="CRISPR">CRISPR</a>, <a href="https://publications.waset.org/abstracts/search?q=ethics" title=" ethics"> ethics</a>, <a href="https://publications.waset.org/abstracts/search?q=regulatory%20framework" title=" regulatory framework"> regulatory framework</a>, <a href="https://publications.waset.org/abstracts/search?q=European%20legislation" title=" European legislation"> European legislation</a> </p> <a href="https://publications.waset.org/abstracts/118942/the-need-for-a-consistent-regulatory-framework-for-crispr-gene-editing-in-the-european-union" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/118942.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">135</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2064</span> Societal Acceptability Conditions of Genome Editing for Upland Rice in Madagascar</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Anny%20Lucrece%20Nlend%20Nkott">Anny Lucrece Nlend Nkott</a>, <a href="https://publications.waset.org/abstracts/search?q=Ludovic%20Temple"> Ludovic Temple</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The appearance in 2012 of the CRISPR-CaS9 genome editing technique marks a turning point in the field of genetics. This technique would make it possible to create new varieties quickly and cheaply. Although some consider CRISPR-CaS9 to be revolutionary, others consider it a potential societal threat. To document the controversy, we explain the socioeconomic conditions under which this technique could be accepted for the creation of a rainfed rice variety in Madagascar. The methodological framework is based on 38 individual and semistructured interviews, a multistakeholder forum with 27 participants, and a survey of 148 rice producers. Results reveal that the acceptability of genome editing requires (i) strengthening the seed system through the operationalization of regulatory structures and the upgrading of stakeholders' knowledge of genetically modified organisms, (ii) assessing the effects of the edited variety on biodiversity and soil nitrogen dynamics, and (iii) strengthening the technical and human capacities of the biosafety body. Structural mechanisms for regulating the seed system are necessary to ensure safe experimentation of genome editing techniques. Organizational innovation also appears to be necessary. The study documents how collective learning between communities of scientists and nonscientists is a component of systemic processes of varietal innovation. This study was carried out with the financial support of the GENERICE project (Generation and Deployment of Genome-Edited, Nitrogen-use-Efficient Rice Varieties), funded by the Agropolis Foundation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR-CaS9" title="CRISPR-CaS9">CRISPR-CaS9</a>, <a href="https://publications.waset.org/abstracts/search?q=varietal%20innovation" title=" varietal innovation"> varietal innovation</a>, <a href="https://publications.waset.org/abstracts/search?q=seed%20system" title=" seed system"> seed system</a>, <a href="https://publications.waset.org/abstracts/search?q=innovation%20system" title=" innovation system"> innovation system</a> </p> <a href="https://publications.waset.org/abstracts/134588/societal-acceptability-conditions-of-genome-editing-for-upland-rice-in-madagascar" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/134588.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">154</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2063</span> Predicting Suicidal Behavior by an Accurate Monitoring of RNA Editing Biomarkers in Blood Samples</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Berengere%20Vire">Berengere Vire</a>, <a href="https://publications.waset.org/abstracts/search?q=Nicolas%20Salvetat"> Nicolas Salvetat</a>, <a href="https://publications.waset.org/abstracts/search?q=Yoann%20Lannay"> Yoann Lannay</a>, <a href="https://publications.waset.org/abstracts/search?q=Guillaume%20Marcellin"> Guillaume Marcellin</a>, <a href="https://publications.waset.org/abstracts/search?q=Siem%20Van%20Der%20Laan"> Siem Van Der Laan</a>, <a href="https://publications.waset.org/abstracts/search?q=Franck%20Molina"> Franck Molina</a>, <a href="https://publications.waset.org/abstracts/search?q=Dinah%20Weissmann"> Dinah Weissmann</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Predicting suicidal behaviors is one of the most complex challenges of daily psychiatric practices. Today, suicide risk prediction using biological tools is not validated and is only based on subjective clinical reports of the at-risk individual. Therefore, there is a great need to identify biomarkers that would allow early identification of individuals at risk of suicide. Alterations of adenosine-to-inosine (A-to-I) RNA editing of neurotransmitter receptors and other proteins have been shown to be involved in etiology of different psychiatric disorders and linked to suicidal behavior. RNA editing is a co- or post-transcriptional process leading to a site-specific alteration in RNA sequences. It plays an important role in the epi transcriptomic regulation of RNA metabolism. On postmortem human brain tissue (prefrontal cortex) of depressed suicide victims, Alcediag found specific alterations of RNA editing activity on the mRNA coding for the serotonin 2C receptor (5-HT2cR). Additionally, an increase in expression levels of ADARs, the RNA editing enzymes, and modifications of RNA editing profiles of prime targets, such as phosphodiesterase 8A (PDE8A) mRNA, have also been observed. Interestingly, the PDE8A gene is located on chromosome 15q25.3, a genomic region that has recurrently been associated with the early-onset major depressive disorder (MDD). In the current study, we examined whether modifications in RNA editing profile of prime targets allow identifying disease-relevant blood biomarkers and evaluating suicide risk in patients. To address this question, we performed a clinical study to identify an RNA editing signature in blood of depressed patients with and without the history of suicide attempts. Patient’s samples were drawn in PAXgene tubes and analyzed on Alcediag’s proprietary RNA editing platform using next generation sequencing technology. In addition, gene expression analysis by quantitative PCR was performed. We generated a multivariate algorithm comprising various selected biomarkers to detect patients with a high risk to attempt suicide. We evaluated the diagnostic performance using the relative proportion of PDE8A mRNA editing at different sites and/or isoforms as well as the expression of PDE8A and the ADARs. The significance of these biomarkers for suicidality was evaluated using the area under the receiver-operating characteristic curve (AUC). The generated algorithm comprising the biomarkers was found to have strong diagnostic performances with high specificity and sensitivity. In conclusion, we developed tools to measure disease-specific biomarkers in blood samples of patients for identifying individuals at the greatest risk for future suicide attempts. This technology not only fosters patient management but is also suitable to predict the risk of drug-induced psychiatric side effects such as iatrogenic increase of suicidal ideas/behaviors. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=blood%20biomarker" title="blood biomarker">blood biomarker</a>, <a href="https://publications.waset.org/abstracts/search?q=next-generation-sequencing" title=" next-generation-sequencing"> next-generation-sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=RNA%20editing" title=" RNA editing"> RNA editing</a>, <a href="https://publications.waset.org/abstracts/search?q=suicide" title=" suicide"> suicide</a> </p> <a href="https://publications.waset.org/abstracts/60650/predicting-suicidal-behavior-by-an-accurate-monitoring-of-rna-editing-biomarkers-in-blood-samples" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/60650.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">258</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2062</span> RNA-Seq Based Transcriptomic Analysis of Wheat Cultivars for Unveiling of Genomic Variations and Isolation of Drought Tolerant Genes for Genome Editing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ghulam%20Muhammad%20Ali">Ghulam Muhammad Ali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Unveiling of genes involved in drought and root architecture using transcriptomic analyses remained fragmented for further improvement of wheat through genome editing. The purpose of this research endeavor was to unveil the variations in different genes implicated in drought tolerance and root architecture in wheat through RNA-seq data analysis. In this study seedlings of 8 days old, 6 cultivars of wheat namely, Batis, Blue Silver, Local White, UZ888, Chakwal 50 and Synthetic wheat S22 were subjected to transcriptomic analysis for root and shoot genes. Total of 12 RNA samples was sequenced by Illumina. Using updated wheat transcripts from Ensembl and IWGC references with 54,175 gene models, we found that 49,621 out of 54,175 (91.5%) genes are expressed at an RPKM of 0.1 or more (in at least 1 sample). The number of genes expressed was higher in Local White than Batis. Differentially expressed genes (DEG) were higher in Chakwal 50. Expression-based clustering indicated conserved function of DRO1and RPK1 between Arabidopsis and wheat. Dendrogram showed that Local White is sister to Chakwal 50 while Batis is closely related to Blue Silver. This study flaunts transcriptomic sequence variations in different cultivars that showed mutations in genes associated with drought that may directly contribute to drought tolerance. DRO1 and RPK1 genes were fetched/isolated for genome editing. These genes are being edited in wheat through CRISPR-Cas9 for yield enhancement. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=transcriptomic" title="transcriptomic">transcriptomic</a>, <a href="https://publications.waset.org/abstracts/search?q=wheat" title=" wheat"> wheat</a>, <a href="https://publications.waset.org/abstracts/search?q=genome%20editing" title=" genome editing"> genome editing</a>, <a href="https://publications.waset.org/abstracts/search?q=drought" title=" drought"> drought</a>, <a href="https://publications.waset.org/abstracts/search?q=CRISPR-Cas9" title=" CRISPR-Cas9"> CRISPR-Cas9</a>, <a href="https://publications.waset.org/abstracts/search?q=yield%20enhancement" title=" yield enhancement"> yield enhancement</a> </p> <a href="https://publications.waset.org/abstracts/107535/rna-seq-based-transcriptomic-analysis-of-wheat-cultivars-for-unveiling-of-genomic-variations-and-isolation-of-drought-tolerant-genes-for-genome-editing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/107535.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">147</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2061</span> A Gold-Based Nanoformulation for Delivery of the CRISPR/Cas9 Ribonucleoprotein for Genome Editing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Soultana%20Konstantinidou">Soultana Konstantinidou</a>, <a href="https://publications.waset.org/abstracts/search?q=Tiziana%20Schmidt"> Tiziana Schmidt</a>, <a href="https://publications.waset.org/abstracts/search?q=Elena%20Landi"> Elena Landi</a>, <a href="https://publications.waset.org/abstracts/search?q=Alessandro%20De%20Carli"> Alessandro De Carli</a>, <a href="https://publications.waset.org/abstracts/search?q=Giovanni%20Maltinti"> Giovanni Maltinti</a>, <a href="https://publications.waset.org/abstracts/search?q=Darius%20Witt"> Darius Witt</a>, <a href="https://publications.waset.org/abstracts/search?q=Alicja%20Dziadosz"> Alicja Dziadosz</a>, <a href="https://publications.waset.org/abstracts/search?q=Agnieszka%20Lindstaedt"> Agnieszka Lindstaedt</a>, <a href="https://publications.waset.org/abstracts/search?q=Michele%20Lai"> Michele Lai</a>, <a href="https://publications.waset.org/abstracts/search?q=Mauro%20Pistello"> Mauro Pistello</a>, <a href="https://publications.waset.org/abstracts/search?q=Valentina%20Cappello"> Valentina Cappello</a>, <a href="https://publications.waset.org/abstracts/search?q=Luciana%20Dente"> Luciana Dente</a>, <a href="https://publications.waset.org/abstracts/search?q=Chiara%20Gabellini"> Chiara Gabellini</a>, <a href="https://publications.waset.org/abstracts/search?q=Piotr%20Barski"> Piotr Barski</a>, <a href="https://publications.waset.org/abstracts/search?q=Vittoria%20Raffa"> Vittoria Raffa</a> </p> <p class="card-text"><strong>Abstract:</strong></p> CRISPR/Cas9 technology has gained the interest of researchers in the field of biotechnology for genome editing. Since its discovery as a microbial adaptive immune defense, this system has been widely adopted and is acknowledged for having a variety of applications. However, critical barriers related to safety and delivery are persisting. Here, we propose a new concept of genome engineering, which is based on a nano-formulation of Cas9. The Cas9 enzyme was conjugated to a gold nanoparticle (AuNP-Cas9). The AuNP-Cas9 maintained its cleavage efficiency in vitro, to the same extent as the ribonucleoprotein, including non-conjugated Cas9 enzyme, and showed high gene editing efficiency in vivo in zebrafish embryos. Since CRISPR/Cas9 technology is extensively used in cancer research, melanoma was selected as a validation target. Cell studies were performed in A375 human melanoma cells. Particles per se had no impact on cell metabolism and proliferation. Intriguingly, the AuNP-Cas9 internalized spontaneously in cells and localized as a single particle in the cytoplasm and organelles. More importantly, the AuNP-Cas9 showed a high nuclear localization signal. The AuNP-Cas9, overcoming the delivery difficulties of Cas9, could be used in cellular biology and localization studies. Taking advantage of the plasmonic properties of gold nanoparticles, this technology could potentially be a bio-tool for combining gene editing and photothermal therapy in cancer cells. Further work will be focused on intracellular interactions of the nano-formulation and characterization of the optical properties. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR%2FCas9" title="CRISPR/Cas9">CRISPR/Cas9</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20editing" title=" gene editing"> gene editing</a>, <a href="https://publications.waset.org/abstracts/search?q=gold%20nanoparticles" title=" gold nanoparticles"> gold nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=nanotechnology" title=" nanotechnology"> nanotechnology</a> </p> <a href="https://publications.waset.org/abstracts/137745/a-gold-based-nanoformulation-for-delivery-of-the-crisprcas9-ribonucleoprotein-for-genome-editing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/137745.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">101</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2060</span> A Pilot Study to Investigate the Use of Machine Translation Post-Editing Training for Foreign Language Learning</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Hong%20Zhang">Hong Zhang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The main purpose of this study is to show that machine translation (MT) post-editing (PE) training can help our Chinese students learn Spanish as a second language. Our hypothesis is that they might make better use of it by learning PE skills specific for foreign language learning. We have developed PE training materials based on the data collected in a previous study. Training material included the special error types of the output of MT and the error types that our Chinese students studying Spanish could not detect in the experiment last year. This year we performed a pilot study in order to evaluate the PE training materials effectiveness and to what extent PE training helps Chinese students who study the Spanish language. We used screen recording to record these moments and made note of every action done by the students. Participants were speakers of Chinese with intermediate knowledge of Spanish. They were divided into two groups: Group A performed PE training and Group B did not. We prepared a Chinese text for both groups, and participants translated it by themselves (human translation), and then used Google Translate to translate the text and asked them to post-edit the raw MT output. Comparing the results of PE test, Group A could identify and correct the errors faster than Group B students, Group A did especially better in omission, word order, part of speech, terminology, mistranslation, official names, and formal register. From the results of this study, we can see that PE training can help Chinese students learn Spanish as a second language. In the future, we could focus on the students’ struggles during their Spanish studies and complete the PE training materials to teach Chinese students learning Spanish with machine translation. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=machine%20translation" title="machine translation">machine translation</a>, <a href="https://publications.waset.org/abstracts/search?q=post-editing" title=" post-editing"> post-editing</a>, <a href="https://publications.waset.org/abstracts/search?q=post-editing%20training" title=" post-editing training"> post-editing training</a>, <a href="https://publications.waset.org/abstracts/search?q=Chinese" title=" Chinese"> Chinese</a>, <a href="https://publications.waset.org/abstracts/search?q=Spanish" title=" Spanish"> Spanish</a>, <a href="https://publications.waset.org/abstracts/search?q=foreign%20language%20learning" title=" foreign language learning"> foreign language learning</a> </p> <a href="https://publications.waset.org/abstracts/100937/a-pilot-study-to-investigate-the-use-of-machine-translation-post-editing-training-for-foreign-language-learning" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/100937.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">144</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2059</span> Investigating the Editing's Effect of Advertising Photos on the Virtual Purchase Decision Based on the Quantitative Electroencephalogram (EEG) Parameters</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Parya%20Tabei">Parya Tabei</a>, <a href="https://publications.waset.org/abstracts/search?q=Maryam%20Habibifar"> Maryam Habibifar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Decision-making is an important cognitive function that can be defined as the process of choosing an option among available options to achieve a specific goal. Consumer ‘need’ is the main reason for purchasing decisions. Human decision-making while buying products online is subject to various factors, one of which is the quality and effect of advertising photos. Advertising photo editing can have a significant impact on people's virtual purchase decisions. This technique helps improve the quality and overall appearance of photos by adjusting various aspects such as brightness, contrast, colors, cropping, resizing, and adding filters. This study, by examining the effect of editing advertising photos on the virtual purchase decision using EEG data, tries to investigate the effect of edited images on the decision-making of customers. A group of 30 participants were asked to react to 24 edited and unedited images while their EEG was recorded. Analysis of the EEG data revealed increased alpha wave activity in the occipital regions (O1, O2) for both edited and unedited images, which is related to visual processing and attention. Additionally, there was an increase in beta wave activity in the frontal regions (FP1, FP2, F4, F8) when participants viewed edited images, suggesting involvement in cognitive processes such as decision-making and evaluating advertising content. Gamma wave activity also increased in various regions, especially the frontal and parietal regions, which are associated with higher cognitive functions, such as attention, memory, and perception, when viewing the edited images. While the visual processing reflected by alpha waves remained consistent across different visual conditions, editing advertising photos appeared to boost neural activity in frontal and parietal regions associated with decision-making processes. These Findings suggest that photo editing could potentially influence consumer perceptions during virtual shopping experiences by modulating brain activity related to product assessment and purchase decisions. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=virtual%20purchase%20decision" title="virtual purchase decision">virtual purchase decision</a>, <a href="https://publications.waset.org/abstracts/search?q=advertising%20photo" title=" advertising photo"> advertising photo</a>, <a href="https://publications.waset.org/abstracts/search?q=EEG%20parameters" title=" EEG parameters"> EEG parameters</a>, <a href="https://publications.waset.org/abstracts/search?q=decision%20Making" title=" decision Making"> decision Making</a> </p> <a href="https://publications.waset.org/abstracts/186491/investigating-the-editings-effect-of-advertising-photos-on-the-virtual-purchase-decision-based-on-the-quantitative-electroencephalogram-eeg-parameters" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186491.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">50</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2058</span> Semi-Automatic Segmentation of Mitochondria on Transmission Electron Microscopy Images Using Live-Wire and Surface Dragging Methods</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mahdieh%20Farzin%20Asanjan">Mahdieh Farzin Asanjan</a>, <a href="https://publications.waset.org/abstracts/search?q=Erkan%20Unal%20Mumcuoglu"> Erkan Unal Mumcuoglu</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mitochondria are cytoplasmic organelles of the cell, which have a significant role in the variety of cellular metabolic functions. Mitochondria act as the power plants of the cell and are surrounded by two membranes. Significant morphological alterations are often due to changes in mitochondrial functions. A powerful technique in order to study the three-dimensional (3D) structure of mitochondria and its alterations in disease states is Electron microscope tomography. Detection of mitochondria in electron microscopy images due to the presence of various subcellular structures and imaging artifacts is a challenging problem. Another challenge is that each image typically contains more than one mitochondrion. Hand segmentation of mitochondria is tedious and time-consuming and also special knowledge about the mitochondria is needed. Fully automatic segmentation methods lead to over-segmentation and mitochondria are not segmented properly. Therefore, semi-automatic segmentation methods with minimum manual effort are required to edit the results of fully automatic segmentation methods. Here two editing tools were implemented by applying spline surface dragging and interactive live-wire segmentation tools. These editing tools were applied separately to the results of fully automatic segmentation. 3D extension of these tools was also studied and tested. Dice coefficients of 2D and 3D for surface dragging using splines were 0.93 and 0.92. This metric for 2D and 3D for live-wire method were 0.94 and 0.91 respectively. The root mean square symmetric surface distance values of 2D and 3D for surface dragging was measured as 0.69, 0.93. The same metrics for live-wire tool were 0.60 and 2.11. Comparing the results of these editing tools with the results of automatic segmentation method, it shows that these editing tools, led to better results and these results were more similar to ground truth image but the required time was higher than hand-segmentation time <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=medical%20image%20segmentation" title="medical image segmentation">medical image segmentation</a>, <a href="https://publications.waset.org/abstracts/search?q=semi-automatic%20methods" title=" semi-automatic methods"> semi-automatic methods</a>, <a href="https://publications.waset.org/abstracts/search?q=transmission%20electron%20microscopy" title=" transmission electron microscopy"> transmission electron microscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=surface%20dragging%20using%20splines" title=" surface dragging using splines"> surface dragging using splines</a>, <a href="https://publications.waset.org/abstracts/search?q=live-wire" title=" live-wire"> live-wire</a> </p> <a href="https://publications.waset.org/abstracts/85965/semi-automatic-segmentation-of-mitochondria-on-transmission-electron-microscopy-images-using-live-wire-and-surface-dragging-methods" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/85965.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">169</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2057</span> Realization of a (GIS) for Drilling (DWS) through the Adrar Region</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Djelloul%20Benatiallah">Djelloul Benatiallah</a>, <a href="https://publications.waset.org/abstracts/search?q=Ali%20Benatiallah"> Ali Benatiallah</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdelkader%20Harouz"> Abdelkader Harouz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Geographic Information Systems (GIS) include various methods and computer techniques to model, capture digitally, store, manage, view and analyze. Geographic information systems have the characteristic to appeal to many scientific and technical field, and many methods. In this article we will present a complete and operational geographic information system, following the theoretical principles of data management and adapting to spatial data, especially data concerning the monitoring of drinking water supply wells (DWS) Adrar region. The expected results of this system are firstly an offer consulting standard features, updating and editing beneficiaries and geographical data, on the other hand, provides specific functionality contractors entered data, calculations parameterized and statistics. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=GIS" title="GIS">GIS</a>, <a href="https://publications.waset.org/abstracts/search?q=DWS" title=" DWS"> DWS</a>, <a href="https://publications.waset.org/abstracts/search?q=drilling" title=" drilling"> drilling</a>, <a href="https://publications.waset.org/abstracts/search?q=Adrar" title=" Adrar "> Adrar </a> </p> <a href="https://publications.waset.org/abstracts/27960/realization-of-a-gis-for-drilling-dws-through-the-adrar-region" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/27960.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">309</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2056</span> Technical Realization of Key Aesthetic Principles in Guzheng Performance</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Qiongzi%20Zheng">Qiongzi Zheng</a>, <a href="https://publications.waset.org/abstracts/search?q=Lewis%20Cornwell"> Lewis Cornwell</a>, <a href="https://publications.waset.org/abstracts/search?q=Neal%20Peres%20Da%20Costa"> Neal Peres Da Costa</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Drawn on Confucian and Taoist philosophy and long-established tradition of aesthetic ideals, the Art of the Chinese Zither (Xishan Qinkuang), a classic work by Chinese music scholar Xu Shangyin in 1643, distilled twenty-four practicing principles for the Chinese zither. This work has influenced the practice of guzheng to the present day. Whilst the principles were described in detail, how they can actually be achieved on a technical level remains to be explored. This study focuses on three key practicing principles: yuan (roundness), liu (fluidness), and su (swiftness), and examines how the playing techniques developed by Master Zhao Manqin contribute to the implementation of the principles. The study concludes that knowledge of the technicality of fingering positioning before and after plucking motion is critical to the realization of these principles. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chinese%20music%20aesthetics" title="Chinese music aesthetics">Chinese music aesthetics</a>, <a href="https://publications.waset.org/abstracts/search?q=practicing%20principles%20of%20the%20Chinese%20zither" title=" practicing principles of the Chinese zither"> practicing principles of the Chinese zither</a>, <a href="https://publications.waset.org/abstracts/search?q=guzheng%20playing%20techniques" title=" guzheng playing techniques"> guzheng playing techniques</a>, <a href="https://publications.waset.org/abstracts/search?q=Zhao%20Manqin%E2%80%99s%20fingering%20techniques" title=" Zhao Manqin’s fingering techniques"> Zhao Manqin’s fingering techniques</a>, <a href="https://publications.waset.org/abstracts/search?q=Xishan%20Qinkuang" title=" Xishan Qinkuang"> Xishan Qinkuang</a> </p> <a href="https://publications.waset.org/abstracts/164771/technical-realization-of-key-aesthetic-principles-in-guzheng-performance" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/164771.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">185</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2055</span> The Effectiveness of Rebranding as a Comparative Study of Ghanaian Business Using the Principles of Corporate Rebranding</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Kennedy%20Gbenu">Kennedy Gbenu</a>, <a href="https://publications.waset.org/abstracts/search?q=Richmond%20Kweku%20Frempong"> Richmond Kweku Frempong </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rebranding has become a very important strategic tool for companies wanting to succeed in the ever competitive business world using the principles of rebranding Moisescu. Two businesses in Ghana (Ghana Commercial Bank and Vodafone Ghana) have been used to ascertain how rebranding of these organizations was done using the principles in their effort to rebrand themselves and to stay relevant. A secondary research mainly on literature surrounding rebranding, official websites of the organizations under study have also been used extensively. After a basic comparative study undertaken two firms (GCB and VODAFONE) seems to be using the first three principles and reaping from it as provided by Moisescu. This goes to show that rebranding should not be done in vacuum but should be guided by such principles so as to achieve the full potential of any kind of investments made. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=brands" title="brands">brands</a>, <a href="https://publications.waset.org/abstracts/search?q=corporate%20branding" title=" corporate branding"> corporate branding</a>, <a href="https://publications.waset.org/abstracts/search?q=innovation" title=" innovation"> innovation</a>, <a href="https://publications.waset.org/abstracts/search?q=case%20studies" title=" case studies"> case studies</a> </p> <a href="https://publications.waset.org/abstracts/20464/the-effectiveness-of-rebranding-as-a-comparative-study-of-ghanaian-business-using-the-principles-of-corporate-rebranding" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20464.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">396</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2054</span> A Simulated Scenario of WikiGIS to Support the Iteration and Traceability Management of the Geodesign Process</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Wided%20Batita">Wided Batita</a>, <a href="https://publications.waset.org/abstracts/search?q=St%C3%A9phane%20Roche"> Stéphane Roche</a>, <a href="https://publications.waset.org/abstracts/search?q=Claude%20Caron"> Claude Caron</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Geodesign is an emergent term related to a new and complex process. Hence, it needs to rethink tools, technologies and platforms in order to efficiently achieve its goals. A few tools have emerged since 2010 such as CommunityViz, GeoPlanner, etc. In the era of Web 2.0 and collaboration, WikiGIS has been proposed as a new category of tools. In this paper, we present WikiGIS functionalities dealing mainly with the iteration and traceability management to support the collaboration of the Geodesign process. Actually, WikiGIS is built on GeoWeb 2.0 technologies —and primarily on wiki— and aims at managing the tracking of participants’ editing. This paper focuses on a simplified simulation to illustrate the strength of WikiGIS in the management of traceability and in the access to history in a Geodesign process. Indeed, a cartographic user interface has been implemented, and then a hypothetical use case has been imagined as proof of concept. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=geodesign" title="geodesign">geodesign</a>, <a href="https://publications.waset.org/abstracts/search?q=history" title=" history"> history</a>, <a href="https://publications.waset.org/abstracts/search?q=traceability" title=" traceability"> traceability</a>, <a href="https://publications.waset.org/abstracts/search?q=tracking%20of%20participants%E2%80%99%20editing" title=" tracking of participants’ editing"> tracking of participants’ editing</a>, <a href="https://publications.waset.org/abstracts/search?q=WikiGIS" title=" WikiGIS"> WikiGIS</a> </p> <a href="https://publications.waset.org/abstracts/54647/a-simulated-scenario-of-wikigis-to-support-the-iteration-and-traceability-management-of-the-geodesign-process" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54647.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">247</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2053</span> A Nucleic Acid Extraction Method for High-Viscosity Floricultural Samples</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Harunori%20Kawabe">Harunori Kawabe</a>, <a href="https://publications.waset.org/abstracts/search?q=Hideyuki%20Aoshima"> Hideyuki Aoshima</a>, <a href="https://publications.waset.org/abstracts/search?q=Koji%20Murakami"> Koji Murakami</a>, <a href="https://publications.waset.org/abstracts/search?q=Minoru%20Kawakami"> Minoru Kawakami</a>, <a href="https://publications.waset.org/abstracts/search?q=Yuka%20Nakano"> Yuka Nakano</a>, <a href="https://publications.waset.org/abstracts/search?q=David%20D.%20Ordinario"> David D. Ordinario</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20W.%20Crawford"> C. W. Crawford</a>, <a href="https://publications.waset.org/abstracts/search?q=Iri%20Sato-Baran"> Iri Sato-Baran</a> </p> <p class="card-text"><strong>Abstract:</strong></p> With the recent advances in gene editing technologies allowing the rewriting of genetic sequences, additional market growth in the global floriculture market beyond previous trends is anticipated through increasingly sophisticated plant breeding techniques. As a prerequisite for gene editing, the gene sequence of the target plant must first be identified. This necessitates the genetic analysis of plants with unknown gene sequences, the extraction of RNA, and comprehensive expression analysis. Consequently, a technology capable of consistently and effectively extracting high-purity DNA and RNA from plants is of paramount importance. Although model plants, such as Arabidopsis and tobacco, have established methods for DNA and RNA extraction, floricultural species such as roses present unique challenges. Different techniques to extract DNA and RNA from various floricultural species were investigated. Upon sampling and grinding the petals of several floricultural species, it was observed that nucleic acid extraction from the ground petal solutions of low viscosity was straightforward; solutions of high viscosity presented a significant challenge. It is postulated that the presence of substantial quantities of polysaccharides and polyphenols in the plant tissue was responsible for the inhibition of nucleic acid extraction. Consequently, attempts were made to extract high-purity DNA and RNA by improving the CTAB method and combining it with commercially available nucleic acid extraction kits. The quality of the total extracted DNA and RNA was evaluated using standard methods. Finally, the effectiveness of the extraction method was assessed by determining whether it was possible to create a library that could be applied as a suitable template for a next-generation sequencer. In conclusion, a method was developed for consistent and accurate nucleic acid extraction from high-viscosity floricultural samples. These results demonstrate improved techniques for DNA and RNA extraction from flowers, help facilitate gene editing of floricultural species and expand the boundaries of research and commercial opportunities. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=floriculture" title="floriculture">floriculture</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20editing" title=" gene editing"> gene editing</a>, <a href="https://publications.waset.org/abstracts/search?q=next-generation%20sequencing" title=" next-generation sequencing"> next-generation sequencing</a>, <a href="https://publications.waset.org/abstracts/search?q=nucleic%20acid%20extraction" title=" nucleic acid extraction"> nucleic acid extraction</a> </p> <a href="https://publications.waset.org/abstracts/191049/a-nucleic-acid-extraction-method-for-high-viscosity-floricultural-samples" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/191049.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">29</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2052</span> ISME: Integrated Style Motion Editor for 3D Humanoid Character</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ismahafezi%20Ismail">Ismahafezi Ismail</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohd%20Shahrizal%20Sunar"> Mohd Shahrizal Sunar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The motion of a realistic 3D humanoid character is very important especially for the industries developing computer animations and games. However, this type of motion is seen with a very complex dimensional data as well as body position, orientation, and joint rotation. Integrated Style Motion Editor (ISME), on the other hand, is a method used to alter the 3D humanoid motion capture data utilised in computer animation and games development. Therefore, this study was carried out with the purpose of demonstrating a method that is able to manipulate and deform different motion styles by integrating Key Pose Deformation Technique and Trajectory Control Technique. This motion editing method allows the user to generate new motions from the original motion capture data using a simple interface control. Unlike the previous method, our method produces a realistic humanoid motion style in real time. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=computer%20animation" title="computer animation">computer animation</a>, <a href="https://publications.waset.org/abstracts/search?q=humanoid%20motion" title=" humanoid motion"> humanoid motion</a>, <a href="https://publications.waset.org/abstracts/search?q=motion%20capture" title=" motion capture"> motion capture</a>, <a href="https://publications.waset.org/abstracts/search?q=motion%20editing" title=" motion editing"> motion editing</a> </p> <a href="https://publications.waset.org/abstracts/54401/isme-integrated-style-motion-editor-for-3d-humanoid-character" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54401.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">382</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2051</span> Design and Implementation of Flexible Metadata Editing System for Digital Contents</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=K.%20W.%20Nam">K. W. Nam</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20J.%20Kim"> B. J. Kim</a>, <a href="https://publications.waset.org/abstracts/search?q=S.%20J.%20Lee"> S. J. Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Along with the development of network infrastructures, such as high-speed Internet and mobile environment, the explosion of multimedia data is expanding the range of multimedia services beyond voice and data services. Amid this flow, research is actively being done on the creation, management, and transmission of metadata on digital content to provide different services to users. This paper proposes a system for the insertion, storage, and retrieval of metadata about digital content. The metadata server with Binary XML was implemented for efficient storage space and retrieval speeds, and the transport data size required for metadata retrieval was simplified. With the proposed system, the metadata could be inserted into the moving objects in the video, and the unnecessary overlap could be minimized by improving the storage structure of the metadata. The proposed system can assemble metadata into one relevant topic, even if it is expressed in different media or in different forms. It is expected that the proposed system will handle complex network types of data. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=video" title="video">video</a>, <a href="https://publications.waset.org/abstracts/search?q=multimedia" title=" multimedia"> multimedia</a>, <a href="https://publications.waset.org/abstracts/search?q=metadata" title=" metadata"> metadata</a>, <a href="https://publications.waset.org/abstracts/search?q=editing%20tool" title=" editing tool"> editing tool</a>, <a href="https://publications.waset.org/abstracts/search?q=XML" title=" XML"> XML</a> </p> <a href="https://publications.waset.org/abstracts/94443/design-and-implementation-of-flexible-metadata-editing-system-for-digital-contents" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/94443.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">171</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2050</span> Islam’s Expediency; The Poison and the Antidote of Islamic Government</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mahdi%20Ebrahimi">Mahdi Ebrahimi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> One of the most effective factors that can empower and modernize the Islamic government according to the needs of society is the institution of expediency in Islamic governance. At the same time, not paying attention to the foundations and principles of Islamic governance may cause this factor to create the basis for corruption and totalitarianism of the rulers, which ultimately causes a gap between the people and the rulers and the emergence of whole government corruption and dictatorship. Meanwhile, there are specific principles in the doctrine of Islamic Governance that can prevent such an event from happening. Principles such as: remaining popular and pious by the Ulama, Amr Bil-Maroof Va Nahy An Al-Munkar (commanding the good and preventing the bad) by the individuals, the rule of frank criticism with loud voices and clarification and accountability in the fields that cause the corruption of those in charge. In this research, these principles are presented along with their Islamic basis. It is also stated what effect each of these cases will have on preserving Islam in the ruling system. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=expediency" title="expediency">expediency</a>, <a href="https://publications.waset.org/abstracts/search?q=Islamic%20ruling" title=" Islamic ruling"> Islamic ruling</a>, <a href="https://publications.waset.org/abstracts/search?q=Islamic%20principles" title=" Islamic principles"> Islamic principles</a>, <a href="https://publications.waset.org/abstracts/search?q=Islam" title=" Islam"> Islam</a> </p> <a href="https://publications.waset.org/abstracts/165483/islams-expediency-the-poison-and-the-antidote-of-islamic-government" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/165483.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">77</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2049</span> Assessing Brain Targeting Efficiency of Ionisable Lipid Nanoparticles Encapsulating Cas9 mRNA/gGFP Following Different Routes of Administration in Mice</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Meiling%20Yu">Meiling Yu</a>, <a href="https://publications.waset.org/abstracts/search?q=Nadia%20Rouatbi"> Nadia Rouatbi</a>, <a href="https://publications.waset.org/abstracts/search?q=Khuloud%20T.%20Al-Jamal"> Khuloud T. Al-Jamal</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Treatment of neurological disorders with modern medical and surgical approaches remains difficult. Gene therapy, allowing the delivery of genetic materials that encodes potential therapeutic molecules, represents an attractive option. The treatment of brain diseases with gene therapy requires the gene-editing tool to be delivered efficiently to the central nervous system. In this study, we explored the efficiency of different delivery routes, namely intravenous (i.v.), intra-cranial (i.c.), and intra-nasal (i.n.), to deliver stable nucleic acid-lipid particles (SNALPs) containing gene-editing tools namely Cas9 mRNA and sgRNA encoding for GFP as a reporter protein. We hypothesise that SNALPs can reach the brain and perform gene-editing to different extents depending on the administration route. Intranasal administration (i.n.) offers an attractive and non-invasive way to access the brain circumventing the blood–brain barrier. Successful delivery of gene-editing tools to the brain offers a great opportunity for therapeutic target validation and nucleic acids therapeutics delivery to improve treatment options for a range of neurodegenerative diseases. In this study, we utilised Rosa26-Cas9 knock-in mice, expressing GFP, to study brain distribution and gene-editing efficiency of SNALPs after i.v.; i.c. and i.n. routes of administration. Methods: Single guide RNA (sgRNA) against GFP has been designed and validated by in vitro nuclease assay. SNALPs were formulated and characterised using dynamic light scattering. The encapsulation efficiency of nucleic acids (NA) was measured by RiboGreen™ assay. SNALPs were incubated in serum to assess their ability to protect NA from degradation. Rosa26-Cas9 knock-in mice were i.v., i.n., or i.c. administered with SNALPs to test in vivo gene-editing (GFP knockout) efficiency. SNALPs were given as three doses of 0.64 mg/kg sgGFP following i.v. and i.n. or a single dose of 0.25 mg/kg sgGFP following i.c.. knockout efficiency was assessed after seven days using Sanger Sequencing and Inference of CRISPR Edits (ICE) analysis. In vivo, the biodistribution of DiR labelled SNALPs (SNALPs-DiR) was assessed at 24h post-administration using IVIS Lumina Series III. Results: Serum-stable SNALPs produced were 130-140 nm in diameter with ~90% nucleic acid loading efficiency. SNALPs could reach and stay in the brain for up to 24h following i.v.; i.n. and i.c. administration. Decreasing GFP expression (around 50% after i.v. and i.c. and 20% following i.n.) was confirmed by optical imaging. Despite the small number of mice used, ICE analysis confirmed GFP knockout in mice brains. Additional studies are currently taking place to increase mice numbers. Conclusion: Results confirmed efficient gene knockout achieved by SNALPs in Rosa26-Cas9 knock-in mice expressing GFP following different routes of administrations in the following order i.v.= i.c.> i.n. Each of the administration routes has its pros and cons. The next stages of the project involve assessing gene-editing efficiency in wild-type mice and replacing GFP as a model target with therapeutic target genes implicated in Motor Neuron Disease pathology. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR" title="CRISPR">CRISPR</a>, <a href="https://publications.waset.org/abstracts/search?q=nanoparticles" title=" nanoparticles"> nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=brain%20diseases" title=" brain diseases"> brain diseases</a>, <a href="https://publications.waset.org/abstracts/search?q=administration%20routes" title=" administration routes"> administration routes</a> </p> <a href="https://publications.waset.org/abstracts/166265/assessing-brain-targeting-efficiency-of-ionisable-lipid-nanoparticles-encapsulating-cas9-mrnaggfp-following-different-routes-of-administration-in-mice" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/166265.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">101</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=editing%20principles&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=editing%20principles&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=editing%20principles&page=4">4</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=editing%20principles&page=5">5</a></li> <li class="page-item"><a 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