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expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); 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digitalDataAnalyticsListener.emailCampaignRecord(); } }); }, formSubmitEvent: function () { var formId = this.getQueryParam("form-submit-id"); if ((formId) && formId !== "" && formId !== null) { var eventDetail = { detail: { form: { id: formId } } }; var formSubmitEvent = new CustomEvent('formSubmit', eventDetail); console.log('form submit event started'); document.dispatchEvent(formSubmitEvent); console.log('form submit event fired', eventDetail); //flush grepToken from Session Storage sessionStorage.removeItem('grepTokenSO'); } }, modalVideoEvent: function (videoId) { if (((videoId) && videoId !== "" && videoId !== null)) { var eventDetail = { detail: { video: { playerId: videoId } } }; var modalVideoEvent = new CustomEvent('videoPlayerLoaded', eventDetail); document.dispatchEvent(modalVideoEvent); } }, customClickEvent: function (clickDetail) { if (((clickDetail) && clickDetail !== "" && clickDetail !== null)) { var eventDetail = { detail: { CustomLink: { linkName: clickDetail } } } var customClickEvent = new CustomEvent('customLinkClicks', eventDetail); document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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alt='Conceptual art of a golden magnetic bead in front of antigens and ligands' title='Exosome isolation, detection, & analysis '></picture><style> #cq-image-jsp-eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_bf08 img{ width:100%; max-width: 300px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_bf08 .image-contain" + "," + "#cq-image-jsp-"+"eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_bf08 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="text parbase section"><p>Exosomes are membrane-secreted vesicles that carry complex nucleic acids, proteins, lipids, and metabolites. Exosomes play key roles in cell-to-cell communication and signal transduction. Because they transport and exchange a variety of cargo between cells, exosomes are used as non-invasive biomarkers for several diseases.</p><p><a class="btn btn-featured" href="#order">Order now</a> <a class="btn btn-primary" href="?open=videos#videos">Watch videos</a></p></div></div></div></div><div style="clear:both"></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="text parbase section"><h3>On this page:</h3><ul><li><a href="#what">What are exosomes?</a></li><li><a href="#isolate">Exosome isolation methods</a></li><li><a href="#detect-analyze">Exosome detection and analysis</a><ul><li><a href="#subpop">Analysis of exosome subpopulations using Dynabeads magnetic beads</a></li><li><a href="#cargo-analyze">Exosome cargo isolation and </a></li><li><a href="#cargo-analyze">Intact exosome analysis</a></li></ul><ul><li><a href="#antibodies">Important antibodies for exosome research</a></li></ul></li><li><a href="#fbs">Exosome-depleted FBS</a></li><li><a href="?open=videos#videos">Videos on exosome isolation, detection, and analysis</a></li><li><a href="#resources">Resources for exosome isolation, detection, and analysis</a></li><li><a href="#order">Order exosome products for isolation, detection, and analysis</a></li></ul></div></div></div></div></div></div><div class="heading section"><h2 id="what"> What are exosomes? </h2></div><div class="text parbase section"><p>Exosomes are a subset of small (30–150 nm) extracellular vesicles containing DNA, RNA, proteins, lipids, and metabolites. Exosomes are constantly secreted by cells <i>in vitro</i> and <i>in vivo</i>. The precise molecular mechanics for their secretion and uptake as well as the composition of their cargo, and resulting functions, are still being discovered. Exosomes have become the focus of growing interest both to study their functions and to understand ways to potentially use them as treatments for diseases.</p></div><div class="heading section"><h3> General functions of exosomes </h3></div><div class="text parbase section"><p>Exosomes’ primary function is to mediate near and long-distance intracellular communication, by transporting their cargo of nucleic acids, proteins, lipids, amino acids, and metabolites, reflecting their cell of origin. Exosomes are being investigated for the key roles they play in the following processes:</p><ul><li>Intracellular communication</li><li>Facilitation of the immune response</li><li>Apoptosis</li><li>Angiogenesis</li><li>Inflammation</li><li>Metastasis and progression of tumors</li><li>Blood coagulation</li><li>Pathological processes in cardiovascular diseases, neurodegeneration, and cancer</li></ul><p> </p></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2"> Selection guide for Dynabeads products </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><p>Dynabeads products are available for a multitude of molecular and cellular applications. Don’t know where to start? Use our easy selection guide that considers sample type, target molecule, and downstream application to provide tailored recommendations for Dynabeads products.</p><p><a class="cta-link" href="/sg/en/home/brands/product-brand/dynal/dynabeads-magnetic-separation-products-selection-guide.html">Dynabeads product selection guide</a></p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab"><a href="/sg/en/home/brands/product-brand/dynal/dynabeads-magnetic-separation-products-selection-guide.html"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.high.jpg/1710339445247.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.480.high.jpg/1710339445247.jpg' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.full.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.480.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.medium.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.620.high.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.320.low.jpg/1710339445247.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_8fa1/uipar/image_44ab/foregroundimg.img.480.medium.jpg/1710339445247.jpg" alt='Conceptual magnetic beads, antibodies, and DNA' title='Dynabeads Products Selection Guide'></picture><style> #cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab img{ width:100%; max-width: 525px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab .image-contain" + "," + "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_8fa1uiparimage_44ab .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="heading section"><h2 id="isolate"><br>Exosome isolation methods </h2></div><div class="heading section"><h3> Exosome isolation using centrifugation </h3></div><div class="text parbase section"><p>Exosome isolation using ultracentrifugation is considered a traditional method of isolation that can be both time-consuming and can damage the exosomes, changing their morphology and functional properties. Exosomes can be separated from cells and heavy artifacts by tying up water molecules via polymer precipitation, thereby reducing the solubility of exosomes and bringing about their precipitation. Exosomes are then harvested following low-speed centrifugation. Total Exosome Isolation reagents and kits utilize this method and help speed up the process of exosome isolation beginning with sample collection—from <a href="/order/catalog/product/4478359">cell culture media</a>, <a href="/order/catalog/product/4478360">serum</a>, <a href="/order/catalog/product/4484450">plasma</a>, <a href="/order/catalog/product/4484452">urine</a>, or <a href="/order/catalog/product/4484453">other body fluids</a>—and ending with purified exosomes ready for downstream molecular analyses (<b>Figure 1</b>). The Total Exosome Isolation kits and reagents, and accompanying protocols, are ideal for a wide range of experiments, including processing low sample volumes and handling multiple samples.</p></div><div class="text parbase section"><div id="crumbs"><ul><li><a><br> Starting sample</a></li><li><a>Incubation with<br> reagents and <br>low-speed <br> centrifugation</a></li><li><a>Molecular assays,<br> biological assays, <br> and intact exosome <br> analysis</a></li></ul></div></div><div class="text parbase section"><p><small><b>Figure 1. Isolation of exosomes using precipitation and low-speed centrifugation.</b></small></p></div><div class="LTRawHTML section"><style> #crumbs { text-align: center; } #crumbs ul { list-style: none; display: inline-table; } #crumbs ul li { display: inline; } #crumbs ul li a { display: block; float: left; height: 95px; background: #3498db; text-align: center; padding: 15px 2px 0 45px; position: relative; margin: 0 10px 0 0; font-size: 15px; text-decoration: none; color: #fff; } #crumbs ul li a:after { content: ""; border-top: 55px solid transparent; border-bottom: 55px solid transparent; border-left: 40px solid #3498db; position: absolute; right: -40px; top: 0; z-index: 1; } #crumbs ul li a:before { content: ""; border-top: 55px solid transparent; border-bottom: 55px solid transparent; border-left: 40px solid #d4f2ff; position: absolute; left: 0; top: 0; } #crumbs ul li:first-child a { border-top-left-radius: 10px; border-bottom-left-radius: 10px; } #crumbs ul li:first-child a:before { display: none; } #crumbs ul li:last-child a { padding-right: 40px; border-top-right-radius: 10px; border-bottom-right-radius: 10px; } #crumbs ul li:last-child a:after { display: none; } /* #crumbs ul li a:hover { background: #fa5ba5; } #crumbs ul li a:hover:after { border-left-color: #fa5ba5; } */ </style></div><div class="heading section"><h3> Magnetic bead-based isolation of exosome sub-populations </h3></div><div class="text parbase section"><p>Dynabeads magnetic beads and DynaGreen magnetic beads are essential tools for isolating and detecting exosomes in various research applications. Magnetic beads offer a simple isolation process from different starting materials such as cell culture media or liquid biopsy samples. Additionally, the KingFisher Sample Purification Systems can automate the process for high throughput or screening.</p><p>The following products include magnetic beads pre-conjugated with antibodies for isolation of specific exosome sub-populations: <a href="/order/catalog/product/10606D">Exosome-Human CD63 Isolation/Detection Reagent</a>, <a href="/order/catalog/product/10616D">Exosome-Human CD81 Isolation Reagent</a>, <a href="/order/catalog/product/10614D">Exosome-Human CD9 Isolation Reagent</a>, and <a href="/order/catalog/product/10618D">Exosome-Human EpCAM Isolation Reagent</a>. These beads isolate exosomes from cell culture media during an overnight incubation.</p><p>To enhance flexibility, biotinylated antibodies can be combined with the <a href="/order/catalog/product/10608D">Exosome-Streptavidin Isolation/Detection Reagent</a> to isolate specific sub-populations using various exosome-specific markers. Alternatively, IgG antibodies from any species can be combined with our sustainable <a href="/order/catalog/product/80107G?SID=srch-srp-80107G">DynaGreen CaptureSelect Anti-IgG-Fc (Multi-Species) Magnetic Beads</a> to capture the exosome of choice. This isolation process is fast, automatable, and efficient, and takes only 40 minutes using liquid biopsy samples such as plasma and urine. Once the exosomes are isolated, they can be further analyzed with western blot, mass spectrometry, qPCR, or electron microscopy (<b>Figure 2</b>).</p></div><div class="image parbase section"><div id="cq-image-jsp-eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_684a"><a class="image-enlarge-link" data-lightbox="image_684a" href="/sg/en/home/life-science/cell-analysis/exosomes/_jcr_content/MainParsys/image_684a/backgroundimg.img.jpg/1728584721303.jpg"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.320.low.jpg/1728584721285.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.320.medium.jpg/1728584721285.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.480.medium.jpg/1728584721285.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.620.high.jpg/1728584721285.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.full.high.jpg/1728584721285.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.320.high.jpg/1728584721285.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.480.high.jpg/1728584721285.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.320.medium.jpg/1728584721285.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.620.high.jpg/1728584721285.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.320.low.jpg/1728584721285.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_684a/foregroundimg.img.480.medium.jpg/1728584721285.jpg" alt='A workflow showing exosome enrichment and detection with Dynabeads magnetic beads' title='Exosome enrichment and detection with Dynabeads magnetic beads'></picture><style> #cq-image-jsp-eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_684a img{ width:100%; max-width: 886px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_684a .image-contain" + "," + "#cq-image-jsp-"+"eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_684a .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div><div class="text parbase section"><small><p><b>Figure 2. Exosome enrichment and detection.</b></p><ol><li>Precipitation with Total Exosome Isolation Kits (specific for cell culture media, urine, plasma, and other samples)</li><li>Charge-based with Dynabeads Intact Virus Enrichment magnetic beads with release</li><li>Dynabeads magnetic beads for sub-population isolation: <ul><li>Exosome-Human CD63 Isolation/Detection Reagent</li><li>Exosome-Human CD81 Isolation Reagent</li><li>Exosome-Human CD9 Isolation Reagent</li><li>Exosome-Human EpCAM Isolation Reagent</li><li>DynaGreen CaptureSelect anti Anti-IgG-Fc (Multi-Species)</li><li>Exosome-Streptavidin Isolation/Detection Reagent</li></ul></li><li>Dynabeads magnetic beads for sub-population flow detection: <ul><li>Exosome-Human CD63 Isolation/Detection Reagent</li><li>Exosome-Human CD81 Flow Detection Reagent</li><li>Exosome-Human CD9 Flow Detection Reagent</li><li>Exosome-Human EpCAM Flow Detection Reagent</li></ul></li><li>Antibodies for western detection: <ul><li>Exosome Anti-Human CD63 antibody</li><li>Exosome Anti-Human CD81 antibody</li><li>Exosome Anti-Human CD9 antibody</li></ul></li></ol></small></div><div class="text parbase section"><p><a class="cta-link" href="?open=order-iso#order-iso">View all exosome isolation products</a></p></div><div class="text parbase section"><p>Exosome isolation from liquid biopsy samples refers to the process of separating exosomes from various body fluids, such as serum (<b>Figure 3</b>), plasma, and urine. The isolation process involves separating exosomes from other components in the liquid biopsy sample, such as cells, proteins, and debris, to obtain a pure exosome population. <a href="/order/catalog/product/10701D">Dynabeads Intact Virus Enrichment</a> isolates exosomes from liquid biopsy samples in less than 15 minutes and enables automation for a high-throughput enrichment. The enrichment is charge-based, thus the negatively charged exosomes in the sample bind to the positively charged Dynabeads magnetic beads. Changing the buffer conditions allows for an easy and quick release step.</p><p>The product can isolate both negatively charged viruses and exosomes.</p></div><div class="image parbase section"><div id="cq-image-jsp-eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_8234"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.320.low.jpg/1728584721329.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.320.medium.jpg/1728584721329.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.480.medium.jpg/1728584721329.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.620.high.jpg/1728584721329.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.full.high.jpg/1728584721329.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.full.high.jpg/1728584721329.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.320.high.jpg/1728584721329.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.480.high.jpg/1728584721329.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.320.medium.jpg/1728584721329.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.620.high.jpg/1728584721329.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.320.low.jpg/1728584721329.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/image_8234/foregroundimg.img.480.medium.jpg/1728584721329.jpg" alt='A diagram showing reversible exosome enrichment from serum including Dynabeads Intact Virus Enrichment' title='A diagram of reversible exosome enrichment from serum with Dynabeads Intact Virus Enrichment'></picture><style> #cq-image-jsp-eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_8234 img{ width:100%; max-width: 1350px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_8234 .image-contain" + "," + "#cq-image-jsp-"+"eec906ea-d723-4130-9b2e-06e137786edfMainParsysimage_8234 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></div></div><div class="text parbase section"><p><small><b>Figure 3. Reversible exosome enrichment from serum. </b>SW480 exosomes were spiked into serum followed by isolation with the Dynabeads Intact Virus Enrichment beads. The exosomes were released from the magnetic beads with 0.25M KI. The serum used was undiluted or diluted to 10% and 50% in PBS. Released exosomes were analyzed by Western blot detecting CD81 (A) or by re-capture by Exosome-Human CD81 Flow Detection Reagent for downstream flow cytometry (B-G). Exosomes spiked into 10%, 50% and 100% serum were captured and released from the Dynabeads Intact Virus Enrichment beads and detected by Western blot (A) and flow cytometry (B-E). CD81 was not detected on the Dynabeads Intact Virus Enrichment beads after release, suggesting that most of the exosomes were eluted off the beads. Exosomes spiked into PBS or 10% serum demonstrated similar isolation and release efficiency (C and D) and in the same range as prior to isolation (B). Interestingly, less dilute serum samples (50% and 100%) resulted in slightly lower capture efficiency (E and F). Exosomes captured from un-spiked and undiluted serum samples were included, demonstrating that a small fraction of the captured exosomes are native exosomes present in the sample (G). Each sample is compared to the isotype control using an irrelevant staining antibody of the same isotype (gray histograms).</small></p></div><div class="LTRawHTML section"><style> #crumbsSmall { text-align: center; } #crumbsSmall ul { list-style: none; display: inline-table; } #crumbsSmall ul li { display: inline; } #crumbsSmall ul li a { display: block; float: left; height: 95px; background: #3498db; text-align: center; padding: 15px 2px 0 45px; position: relative; margin: 0 10px 0 0; font-size: 12px; text-decoration: none; color: #fff; } #crumbsSmall ul li a:after { content: ""; border-top: 55px solid transparent; border-bottom: 55px solid transparent; border-left: 40px solid #3498db; position: absolute; right: -40px; top: 0; z-index: 1; } #crumbsSmall ul li a:before { content: ""; border-top: 55px solid transparent; border-bottom: 55px solid transparent; border-left: 40px solid #d4f2ff; position: absolute; left: 0; top: 0; } #crumbsSmall ul li:first-child a { border-top-left-radius: 10px; border-bottom-left-radius: 10px; } #crumbsSmall ul li:first-child a:before { display: none; } #crumbsSmall ul li:last-child a { padding-right: 40px; border-top-right-radius: 10px; border-bottom-right-radius: 10px; } #crumbsSmall ul li:last-child a:after { display: none; } /* #crumbsSmall ul li a:hover { background: #fa5ba5; } #crumbsSmall ul li a:hover:after { border-left-color: #fa5ba5; } */ </style></div><div class="heading section"><h2 id="detect-analyze"> Exosome detection and analysis </h2></div><div class="text parbase section"><p>Detection and analysis of exosomes can be very challenging due to their small size (30–150 nm). However, there are a few methods used for exosome detection and analysis.</p><ul><li><b>Nanoparticle tracking analysis (NanoSight instrument)</b>—Exosomes recovered with <a href="?open=order-iso#order-iso">Total Exosome Isolation</a> reagents and kits can be sized and counted using a NanoSight instrument or similar tool, such as ParticleMetrix, Izon, and Malvern.</li><li><b>Electron microscopy (EM)</b>—One of the advantages of using Dynabeads magnetic beads to purify exosomal subpopulations is that you can directly perform EM analysis. You can analyze subsets from total exosomes enriched from cell culture using <a href="/order/catalog/product/10614D">Exosome-Human CD9 Isolation Reagent</a>-, <a href="/order/catalog/product/10606D">Exosome-Human CD63 Isolation/Detection Reagent</a>-, <a href="/order/catalog/product/10616D">Exosome-Human CD81 Isolation Reagent</a>-, or <a href="/order/catalog/product/10618D">Exosome-Human EpCAM Isolation Reagent</a>, or have the option to use the <a href="/order/catalog/product/10608D">Streptavidin reagent</a> in combination with your choice of biotinylated antibody.</li><li><b>Flow cytometry</b>—Immunomagnetic capture of exosomes via Dynabeads magnetic beads help allow for clear and defined FFC/SSC detection (typically in less than one hour). You can analyze subset exosome populations using the <a href="/order/catalog/product/10606D">Exosome-Human CD63 Isolation/Detection Reagent</a>, <a href="/order/catalog/product/10620D">Exosome-Human CD9 Flow Detection Reagent,</a> <a href="/order/catalog/product/10622D">Exosome-Human CD81 Flow Detection Reagent</a>, or <a href="/order/catalog/product/10624D">Exosome-Human EpCAM Flow Detection Reagent</a></li><li><b>Dynamic light scattering</b></li><li><b>HS flow cytometry</b></li><li><b>Resistive pulse sensing (Spectradyne)</b></li><br></ul></div><div class="text parbase section"><p><a class="cta-link" href="?open=order-det#order-det">View all exosome detection and analysis products</a></p></div><div class="heading section"><h2 id="cargo-analyze"> Exosome cargo isolation and intact exosome analysis </h2></div><div class="text parbase section"><p>Exosomes have been shown to transport a range of molecules between different cell types. Their cargo includes various proteins, mRNA (fragments and full length), rRNA, tRNA, miRNA, and ncRNA.</p><ul><li><b>RNA isolation</b>—Purification of total RNA, or recovery of both protein and RNA from the same sample of pre-enriched exosomes, can be achieved using the Invitrogen <a href="/order/catalog/product/4478545">Total Exosome RNA and Protein Isolation Kit</a>. The kit is compatible with all protocols for exosome isolation.</li><li><b>Protein isolation</b>—Exosome Immunoprecipitation Reagents (<a href="/order/catalog/product/10610D">Protein A</a> or <a href="/order/catalog/product/10612D">Protein G</a>) are designed for the immunoprecipitation of proteins, protein complexes, protein-nucleic acid complexes, and other exosome-related antigens, which can then be used in molecular assays and intact exosome analysis.</li><li><b>Protein analysis</b>—Specific monoclonal antibodies (<a href="/antibody/product/CD9-Antibody-clone-Ts9-Monoclonal/10626D">Exosome Anti-Human CD9</a>, <a href="/antibody/product/CD63-Antibody-clone-Ts63-Monoclonal/10628D">Exosome Anti-Human CD63</a>, and <a href="/antibody/product/CD81-Antibody-clone-M38-Monoclonal/10630D">Exosome Anti-Human CD81</a>) allow for detection of cellular and exosomal antigens by Western blot analysis. If you are looking to compare multiple protein samples on the same gel and want to keep exosomal protein complexes intact, you may use the Dynabeads-based immunoprecipitation method using <a href="/order/catalog/product/10610D">Protein A</a> or <a href="/order/catalog/product/10612D">Protein G</a>.</li><li><b>RNA analysis</b>—Exosomal RNA and membrane components can be labeled using fluorescent dyes, while unincorporated dye can be easily depleted with Invitrogen spin columns. Exosomal RNA recovered using the <a href="/order/catalog/product/4478545">Total Exosome RNA and Protein Isolation Kit</a> can be analyzed by qRT-PCR using Applied Biosystems <a href="/sg/en/home/life-science/pcr/real-time-pcr/real-time-pcr-assays.html">TaqMan Assays</a>, <a href="/sg/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-applications/rna-sequencing-sample-preparation.html">RNA sequencing</a>, or <span class="icon-lt-pdf"></span> <a href="https://assets.thermofisher.com/TFS-Assets/LSG/manuals/small_RNA_library_sequencing_man.pdf" target="_blank">next-generation sequencing tools</a>. Exosomal RNA and membrane components can be labeled using fluorescent dyes, while unincorporated dye can be removed via spin columns.</li></ul><p> </p></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt8"><div class="parsys_column cq-colctrl-lt8-c0"><div container-id="cq-colctrl-lt8" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-eec906ea-d723-4130-9b2e-06e137786edfMainParsyswell_1e40uiparimage_5cfc"><a href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/fbs/specialty-serum.html?open=exosome-depleted#exosome-depleted.html"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/well_1e40/uipar/image_5cfc/foregroundimg.img.full.high.jpg/1728584721463.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/well_1e40/uipar/image_5cfc/foregroundimg.img.full.high.jpg/1728584721463.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/well_1e40/uipar/image_5cfc/foregroundimg.img.320.high.jpg/1728584721463.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/well_1e40/uipar/image_5cfc/foregroundimg.img.480.high.jpg/1728584721463.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/well_1e40/uipar/image_5cfc/foregroundimg.img.320.medium.jpg/1728584721463.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/well_1e40/uipar/image_5cfc/foregroundimg.img.620.high.jpg/1728584721463.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/well_1e40/uipar/image_5cfc/foregroundimg.img.320.low.jpg/1728584721463.jpg,https://www.thermofisher.com/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/well_1e40/uipar/image_5cfc/foregroundimg.img.480.medium.jpg/1728584721463.jpg" alt='Exosome depleted FBS product photo' title='Exosome depleted FBS '></picture><style> #cq-image-jsp-eec906ea-d723-4130-9b2e-06e137786edfMainParsyswell_1e40uiparimage_5cfc img{ width:100%; max-width: 350px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"eec906ea-d723-4130-9b2e-06e137786edfMainParsyswell_1e40uiparimage_5cfc .image-contain" + "," + "#cq-image-jsp-"+"eec906ea-d723-4130-9b2e-06e137786edfMainParsyswell_1e40uiparimage_5cfc .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div><div class="parsys_column cq-colctrl-lt8-c1"><div container-id="cq-colctrl-lt8" comp-id="B1"><div class="heading section"><div class="h2" id="fbs"> Exosome-depleted FBS </div></div></div><div container-id="cq-colctrl-lt8" comp-id="B2"><div class="text parbase section"><p>By using exosome-depleted FBS, the inefficiencies that come with ultracentrifugation of serum are lessened. Gibco exosome-depleted FBS has the highest level of exosome depletion compared to homebrewed material and competitor FBS while retaining the vital nutrients necessary for cell culture. Features of this specialty FBS include:</p><ul><li>≥90% of exosomes depleted</li><li>Cell culture testing of every lot</li><li>Specially developed for exosome research</li></ul><p><a class="cta-link" href="/sg/en/home/life-science/cell-culture/mammalian-cell-culture/fbs/specialty-serum.html?open=exosome-depleted#exosome-depleted">Learn more about Gibco exosome-depleted FBS</a></p></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><div class="h2" id="automate-sample-preparation"> Automate your sample preparation using Dynabeads magnetic beads with protocols for Kingfisher instruments </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="text parbase section"><p>KingFisher instruments automate the isolation of DNA, RNA, proteins, exosomes, viruses, and cells. KingFisher instruments help eliminate cross contamination, produce reproducible results, and cut your hands-on time down to 40 minutes. Set up plates, press start, and walk away.</p><p><a class="cta-link" href="/sg/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/kingfisher-systems.html">Learn more about KingFisher instruments for automated protocols</a></p></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="image parbase section"><div id="cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_92dcuiparimage_16f3"><a href="/sg/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/kingfisher-systems.html"><picture><source srcset='https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.full.high.jpg/1710339445067.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.full.high.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.320.high.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.480.high.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.320.medium.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.620.high.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.320.low.jpg/1710339445067.jpg,https://www.thermofisher.com/sg/en/reference-components/bid-reference-components/sample-prep-resource-boxes/jcr:content/MainParsys/well_92dc/uipar/image_16f3/foregroundimg.img.480.medium.jpg/1710339445067.jpg" alt='Three KingFisher automation family of instruments: Duo Prime, Flex, and Presto' title='Automated protocols with KingFisher instruments'></picture><style> #cq-image-jsp-fd598175-dace-4391-9fef-15325671dabcMainParsyswell_92dcuiparimage_16f3 img{ width:100%; max-width: 234px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_92dcuiparimage_16f3 .image-contain" + "," + "#cq-image-jsp-"+"fd598175-dace-4391-9fef-15325671dabcMainParsyswell_92dcuiparimage_16f3 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div><div class="accordion section"><div class="accordion" id="bcadc42a76004d8db9fd4df7a95e1612" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#bcadc42a76004d8db9fd4df7a95e1612" href="#videos"><span class="caret"></span> Exosome videos </a></div><div class="accordion-body collapse " id="videos"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="heading section"><h2> Videos on exosome isolation, detection, and analysis </h2></div><div class="heading section"><h3> Documentary mini-series: Exosomes—The Next Small Thing </h3></div><div class="text parbase section"><p>In this documentary mini-series, we asked ten prominent scientists to share their thoughts on exosome research.</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><p>Watch all six episodes now:</p><ol><li><a href="/sg/en/home/life-science/cell-analysis/exosomes/exosomes-documentary-episode-1.html">What is an Exosome?</a></li><li><a href="/sg/en/home/life-science/cell-analysis/exosomes/exosomes-documentary-episode-2.html">The History and Promise of Exosomes</a></li><li><a href="/sg/en/home/life-science/cell-analysis/exosomes/exosomes-documentary-episode-3.html">Exosomes in Cancer Research</a></li><li><a href="/sg/en/home/life-science/cell-analysis/exosomes/exosomes-documentary-episode-4.html">Curiosity and a Passion for Science</a></li><li><a href="/sg/en/home/life-science/cell-analysis/exosomes/exosomes-documentary-series-episode-5.html">Collaboration—The Key to Scientific Success</a></li><li><a href="/sg/en/home/life-science/cell-analysis/exosomes/exosomes-documentary-episode-6.html">Exosomes—The Next Small Thing</a></li></ol></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="brightcoveplayer section"><div data-video="83ab455f9067"><script type="text/javascript"> var loadEME = true; 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margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-83ab455f9067" class="brightcove-container"><script>document.createElement('video')</script><video id="video-83ab455f9067" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="3808163873001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "ISEV 2013 - RNA profiling of exosomes, Sasha Vlassov", "description": "Oral presentation at ISEV 2013 in Boston, by Life Technologies scientist Sasha Vlassov. 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="heading section"><h3> Video: Poster presentation on exosome isolation based on surface protein expression </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="brightcoveplayer section"><div data-video="3be812cc40bf"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-3be812cc40bf', 10); </script></div><div class="video-container video-single"><div id="span-video-3be812cc40bf" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-3be812cc40bf" class="brightcove-container"><script>document.createElement('video')</script><video id="video-3be812cc40bf" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="3808197242001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "ISEV 2013 Poster Session - Exosome Isolation and characterization, Axl Neurauter", "description": "Poster presentation at ISEV 2013 in Boston. 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div></div><div style="clear:both"></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="heading section"><h3> Video: Poster presentation on RNA characterization of human blood-derived exosomes </h3></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="brightcoveplayer section"><div data-video="1aba31e73b9b"><script type="text/javascript"> var loadEME = true; function checkVideojsLoaded(callback, schemaVideoId, maxIterations) { let iterations = 0; return new Promise((resolve, reject) => { function iterate() { if(loadEME && typeof videojs != "undefined"){ videojs.registerPlugin('eme', function() {}); loadEME = false; } // Perform your value check here if (typeof videojs!="undefined" && videojs.getPlayer(schemaVideoId)) { resolve(); // Resolve the promise if the value is true } else if (iterations <= (maxIterations*1e3)) { iterations++; setTimeout(iterate, 500); // Wait for 0.5 second and iterate again } else { reject('Maximum iteration time exceeded.'); // Reject the promise if max iterations reached } } iterate(); // Start the iteration }) .then(() => { callback(); // Call the provided callback function when the value is true }) .catch((error) => { console.error('Error:', error); }); } checkVideojsLoaded(function(){ videojs.options.techOrder = ["html5"]; }, 'video-1aba31e73b9b', 10); </script></div><div class="video-container video-single"><div id="span-video-1aba31e73b9b" class="video-player-wrapper"><div style="margin-bottom: 0; margin-left: auto; margin-right: auto; margin-top: 0; overflow-x: hidden; overflow-y: hidden; text-align: center; width: 100%; text-align: center;"><div id="container-1aba31e73b9b" class="brightcove-container"><script>document.createElement('video')</script><video id="video-1aba31e73b9b" data-account="3663210762001" data-player="08UsfMRkC" data-embed="default" data-video-id="3872837398001" class="video-js" controls ></video><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script> videojs.options.techOrder = ["html5"]; </script></div></div></div></div><script src="//players.brightcove.net/3663210762001/08UsfMRkC_default/index.min.js"></script><script type="text/javascript"> var schemaObj = { "@context": "https://schema.org", "@type": "VideoObject", "name": "Ion Torrent AGBT Poster - Characterization of RNA content of human blood-derived exosomes using PGM", "description": "Dr. Robert Setterquist presents a Life Technologies poster at AGBT 2012: Characterization of the RNA content of the human blood-dreived exosomes using the Ion Personal Genome Machine.", "thumbnailUrl": ["https://cf-images.us-east-1.prod.boltdns.net/v1/static/3663210762001/0128d109-ded9-41f1-9df6-59ba5b128e43/894c1657-4cde-4a41-b149-38e0e9f187f0/160x90/match/image.jpg"], "uploadDate": "2014-11-03T17:56:22.071Z", "duration": "PT5M38.756S", "contentUrl": "https://edge.api.brightcove.com/playback/v1/accounts/3663210762001/videos/3872837398001/high.mp4?bcov_auth=eyJhbGciOiAiUlMyNTYiLCJ0eXAiOiAiSldUIn0.ewogICJpc3MiOiAiaHR0cHM6Ly93d3cudGhlcm1vZmlzaGVyLmNvbS8iLAogICJzdWIiOiAiY29udGVudF91cmwiLAogICJhdWQiOiAiaHR0cHM6Ly9icmlnaHRjb3ZlLmNvbSIKfQ.YqHusDPupPtMxztEAJqXZdheYqO3snN6xjms4Ui3zIPoj2yxzflQiCo4F8WHxA9NYzu3QGhPfCS_m8RFrLoa656z7RNfPGWsxJRa-IPjFvLAqhwJiJNWKBMoClWyGj0-groti1dghXWuGXxNraTU5GZ92tReZd9H8-g1ugo2hP_CV_qPtN8_uk6049UR1uXSXkiVf-bHrShqG1GDrTELzsHkLy3QAbyFkSlITfeaKcqGQO0nmAMPxOPbqHF5WjQwJEZyRmHOOGLXcy1PMRSUvtT9LUpJ6NOz8wgw6dfLpqdxnZOuSxwxTC6WRcnRSiokgpYuP6zYCopaoWUkeIzX3Q", "embedUrl": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=3872837398001", "@id": "//players.brightcove.net/3663210762001/08UsfMRkC_default/index.html?videoId=3872837398001&_="+new Date().getTime() }; var el = document.createElement("script"); el.type = "application/ld+json"; el.text = JSON.stringify(schemaObj); document.head.appendChild(el); if (navigator.userAgent.indexOf('MSIE') !== -1 || navigator.userAgent.indexOf('Trident/') !== -1) { $('.brightcoveplayer, .cmp-p-videoplayer').addClass('brightcoveplayer-ie'); $("<div class='row' />") .append("<div class='span1 warning-triangle-icon' />") .append("<div class='span11'><b>Error:</b> Internet Explorer is not compatible with the Brightcove Video Player. 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Please use a different browser to view these videos.</div>") .addClass("unsuppported-videoplayer") .insertAfter($(".brightcoveplayer, .cmp-p-videoplayer")); } </script></div></div></div></div><div style="clear:both"></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#bcadc42a76004d8db9fd4df7a95e1612').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#bcadc42a76004d8db9fd4df7a95e1612').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="text parbase section"><p><a name="celebrate"></a></p></div><div class="heading section"><h2 id="order"> Order exosome products for isolation, detection, and analysis </h2></div><div class="accordion section"><div class="accordion" id="b77ebcceab8641c0a461dc10370c5763" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#b77ebcceab8641c0a461dc10370c5763" href="#orderiso"><span class="caret"></span> Total exosome isolation </a></div><div class="accordion-body collapse " id="orderiso"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">4478359, 4478360, 4484450, 4484452, 4484453, 4478545, 10610D, 10612D, 10608D, 10614D, 10606D, 10616D, 10618D, 10700D, 10701D, 80107G, 80108G, 80109G</div><div id='ltajax-7546016728035253390'></div><script type="text/javascript">(function() {$('#ltajax-7546016728035253390').load('/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/accordion_602d/itemspar/accordionitem_e7e3/itemParsys/productlist_5c1b.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#b77ebcceab8641c0a461dc10370c5763" href="#orderdet"><span class="caret"></span> Exosome detection and analysis of subpopulations and cargo </a></div><div class="accordion-body collapse " id="orderdet"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="productlist parbase section"><div id="skulist" style="display:none">10610D, 10612D, 10608D, 10614D, 10606D, 10616D, 10618D, 10620D, 10622D, 10624D, 10626D, 10628D, 10630D, S32703, D7540</div><div id='ltajax-8526840226025159255'></div><script type="text/javascript">(function() {$('#ltajax-8526840226025159255').load('/sg/en/home/life-science/cell-analysis/exosomes/jcr:content/MainParsys/accordion_602d/itemspar/accordionitem_4681/itemParsys/productlist_823b.ajax.html?skulist=');})(); </script></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#b77ebcceab8641c0a461dc10370c5763').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#b77ebcceab8641c0a461dc10370c5763').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h2 id="related-content"> Exosome isolation, detection, and analysis related content </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><h3>Frequently asked questions (FAQs)</h3><ul class="the-icons clearfix"><li><a href="/sg/en/home/life-science/cell-analysis/exosomes/video-faqs-exosomes.html">FAQs about exosomes in video format</a></li><li><a href="/sg/en/home/life-science/cell-analysis/exosomes/exosomes-webinar-q-and-a.html">Exosome FAQs tool</a></li></ul></div></div><div container-id="cq-colctrl-lt0" comp-id="A2"><div class="text parbase section"><h3>Application notes</h3><ul class="the-icons clearfix"><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/isolating-highly-pure-exosome-subpopulations-western-blot-app-note.pdf" target="_blank">Tips for isolating highly pure exosome subpopulations for western blot</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/ultrastructural-analysis-exosomes-immunolabeling-negative-staining-app-note.pdf" target="_blank">Ultrastructural analysis of exosomes by immunolabeling and negative staining</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/brochures/automated-pull-down-extracellular-vesicles-app-note.pdf" target="_blank">Automated pull-down of extracellular vesicles (EVs) on the KingFisher system using Dynabeads magnetic beads—standardizing EV capture and analysis</a></li><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/Exosome%20Tracing_App%20Note.pdf" target="_blank">Labeling exosomal RNA and membrane components using fluorescent dyes</a></li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><h3>Flyer</h3><ul class="the-icons clearfix"><li><span class="icon-lt-pdf"></span><a href="https://assets.thermofisher.com/TFS-Assets/BID/Flyers/take-closer-look-exosomes-flyer.pdf" target="_blank">Take a closer look at exosomes</a></li></ul></div></div><div container-id="cq-colctrl-lt0" comp-id="B2"><div class="text parbase section"><h3>Blog</h3><ul class="the-icons clearfix"><li><span class="icon icon-outbound"></span><a href="/blog/behindthebench/using-exosomes-and-cell-free-dna-in-cancer-research-an-interview-with-katie-meehan/" target="_blank">Using Exosomes and Cell-Free DNA in Cancer Research—an Interview with Katie Meehan</a></li></ul></div></div><div container-id="cq-colctrl-lt0" comp-id="B3"><div class="text parbase section"><h3>Webinars</h3><ul class="the-icons clearfix"><li><a href="/sg/en/home/life-science/cell-analysis/exosomes/exosomes-webinars.html">Specific isolation and analysis of exosome sub-populations</a></li><li><a href="/sg/en/home/life-science/cell-analysis/exosomes/exosomes-webinars.html">Exosomes isolation and monitoring: From cell culture to clinically relevant research samples</a></li></ul></div></div></div></div><div style="clear:both"></div></div></div></div></div></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well "><div class="well-container" style=""><div class="well-content"><div class="parsys"><div 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