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ssDNA | Single-Stranded DNA Synthesis | GENEWIZ from Azenta
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href='https://www.azenta.com/' style='color:#826ec3;font-size:17px'>Azenta Life Sciences – Services</a></li><br><li><a class='GColor' href='/en/Public/Services/Next-Generation-Sequencing' style='color:#826ec3;font-size:17px'>Next Generation Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/RNA-Seq' style='color:#585F69;font-size:14px'>RNA-Seq</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Proteomics' style='color:#585F69;font-size:14px'>Proteomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Gene-Expression-Screening' style='color:#585F69;font-size:14px'>High-Throughput Gene Expression Screening <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Single-Cell-Sequencing' style='color:#585F69;font-size:14px'>Single-Cell Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Exome-Sequencing' style='color:#585F69;font-size:14px'>Exome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Amplicon-Sequencing-Services' style='color:#585F69;font-size:14px'>Amplicon Sequencing Services</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Standalone-NGS-Solutions' style='color:#585F69;font-size:14px'>Sequencing Only<br></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/High-Throughput-Genotyping' style='color:#585F69;font-size:14px'>CRISPR Validation</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels' style='color:#585F69;font-size:14px'>Targeted Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Metagenomics-Solutions' style='color:#585F69;font-size:14px'>Metagenomics </a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Epigenomics' style='color:#585F69;font-size:14px'>Epigenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Antibody-Discovery' style='color:#585F69;font-size:14px'>Antibody Discovery <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Immunogenomics' style='color:#585F69;font-size:14px'>Immunogenomics</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Digital-Spatial-Profiling' style='color:#585F69;font-size:14px'>Digital Spatial Profiling</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/AAV-Genome-Sequencing' style='color:#585F69;font-size:14px'>AAV Genome Sequencing</a></li><li><a href='/en/Public/Services/Next-Generation-Sequencing/Whole-Plasmid-Sequencing-Plasmid-EZ' style='color:#585F69;font-size:14px'>Whole Plasmid Sequencing: Plasmid-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Sanger-Sequencing' style='color:#826ec3;font-size:17px'>Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Sanger-EZ' style='color:#585F69;font-size:14px'>Sanger-EZ <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Sanger-Sequencing/AAV-ITR' style='color:#585F69;font-size:14px'>AAV-ITR Sanger Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Purified-Templates' style='color:#585F69;font-size:14px'>Purified Templates</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Difficult-Template-Sequencing' style='color:#585F69;font-size:14px'>Difficult Template Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Direct-Colony-Sequencing' style='color:#585F69;font-size:14px'>Direct Colony Sequencing</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/PCR-Purification' style='color:#585F69;font-size:14px'>PCR Purification</a></li><li><a href='/en/Public/Services/Sanger-Sequencing/Primer-Walking' style='color:#585F69;font-size:14px'>Primer Walking</a></li><br><li><a class='GColor' href='/en/Public/Services/Molecular-Genetics' style='color:#826ec3;font-size:17px'>PCR + Sanger Services</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Bacterial-and-Fungal-Identification' style='color:#585F69;font-size:14px'>Bacterial and Fungal Identification</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Mutation-Analysis' style='color:#585F69;font-size:14px'>Mutation Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/SNP-Genotyping' style='color:#585F69;font-size:14px'>SNP Genotyping</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Plus-Sequencing-CRISPR-Analysis-Package' style='color:#585F69;font-size:14px'>PCR Plus Sequencing CRISPR Analysis Package</a></li><li><a href='/en/Public/Services/Molecular-Genetics/cDNA-Verification' style='color:#585F69;font-size:14px'>Confirmatory Sequencing & Cell Bank Characterization</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Yeast-Colony-Sequencing' style='color:#585F69;font-size:14px'>Yeast Colony Sequencing</a></li><li><a href='/en/Public/Services/Molecular-Genetics/Fragment-Analysis' style='color:#585F69;font-size:14px'>Fragment Analysis</a></li><li><a href='/en/Public/Services/Molecular-Genetics/PCR-Solutions' style='color:#585F69;font-size:14px'>Quantitative PCR & Digital PCR <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Molecular-Genetics/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>Nucleic Acid Extraction</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Gene-Synthesis' style='color:#826ec3;font-size:17px'>DNA & RNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Synthesis' style='color:#585F69;font-size:14px'>Gene Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Antibody-DNA-Synthesis' style='color:#585F69;font-size:14px'>Antibody Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/AAV-Plasmid-Synthesis' style='color:#585F69;font-size:14px'>AAV Plasmid Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Gene-Fragments' style='color:#585F69;font-size:14px'>Gene Fragments</a></li><li><a href='/en/Public/Services/Gene-Synthesis/CRISPR-Construct-Synthesis' style='color:#585F69;font-size:14px'>CRISPR Construct Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Synthetic-DNA-Libraries' style='color:#585F69;font-size:14px'>Synthetic DNA Libraries</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Single-Stranded-DNA-Synthesis' style='color:#585F69;font-size:14px'>ssDNA Synthesis</a></li><li><a href='/en/Public/Services/Gene-Synthesis/DNA-Cloning' style='color:#585F69;font-size:14px'>DNA Cloning</a></li><li><a href='/en/Public/Services/Gene-Synthesis/Site-Directed-Mutagenesis' style='color:#585F69;font-size:14px'>Site-Directed Mutagenesis<br></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Viral-Package' style='color:#585F69;font-size:14px'>Viral Packaging <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Recombinant-Antibody-Production' style='color:#585F69;font-size:14px'>Recombinant Antibody Production <sup style="color:#826ec3;"><strong>NEW </strong></sup></a></li><li><a href='/en/Public/Services/Gene-Synthesis/Custom-mRNA-Synthesis' style='color:#585F69;font-size:14px'>Custom mRNA Synthesis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><br><li><a class='GColor' href='/en/Public/Services/Oligo' style='color:#826ec3;font-size:17px'>Oligo Synthesis Services</a></li><li><a href='/en/Public/Services/Oligo/Overnight-DNA-Oligos' style='color:#585F69;font-size:14px'>Overnight DNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Custom-DNA-and-RNA-Oligos' style='color:#585F69;font-size:14px'>Custom DNA and RNA Oligos</a></li><li><a href='/en/Public/Services/Oligo/Oligo-Retained-for-Sanger-Sequencing' style='color:#585F69;font-size:14px'>Oligo Retained for Sanger Sequencing</a></li></ul></div><div class='col-md-3 '><ul class='list-unstyled'><li><a class='GColor' href='/en/Public/Services/Plasmid-DNA-Prep' style='color:#826ec3;font-size:17px'>Plasmid DNA Preparation</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Standard' style='color:#585F69;font-size:14px'>Standard Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/Targeted-Yield' style='color:#585F69;font-size:14px'>Targeted Yield Plasmid DNA Prep</a></li><li><a href='/en/Public/Services/Plasmid-DNA-Prep/AAV-Plasmid-Preparation' style='color:#585F69;font-size:14px'>AAV Plasmid Preparation</a></li><br><li><a class='GColor' href='/en/Public/Services/Clinical-Services' style='color:#826ec3;font-size:17px'>PreClinical and Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Clinical-Services-Overview' style='color:#585F69;font-size:14px'>Clinical Services</a></li><li><a href='/en/Public/Services/Clinical-Services/Microarray-Solutions' style='color:#585F69;font-size:14px'>Microarray Solutions <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Variant-Confirmation' style='color:#585F69;font-size:14px'>CLIA Variant Confirmation (PCR + Sanger)</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Sanger-Sequencing' style='color:#585F69;font-size:14px'>CLIA Sanger Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Exome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Exome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Whole-Genome-Sequencing' style='color:#585F69;font-size:14px'>CLIA Whole Genome Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/Regulatory' style='color:#585F69;font-size:14px'>GLP-Compliant Overview</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Confirmatory-Sequencing' style='color:#585F69;font-size:14px'>GLP Confirmatory Sequencing</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-SNP-Mutation-Analysis' style='color:#585F69;font-size:14px'>GLP SNP Mutation Analysis</a></li><li><a href='/en/Public/Services/Clinical-Services/GLP-compliant-Plasmid-Prep' style='color:#585F69;font-size:14px'>GLP Plasmid Prep</a></li><li><a href='/en/Public/Services/Clinical-Services/Nucleic-Acid-Extraction' style='color:#585F69;font-size:14px'>GLP Nucleic Acid Extraction</a></li><li><a href='/en/Public/Services/Clinical-Services/Biofluid-Processing-Overview' style='color:#585F69;font-size:14px'>Biofluid Processing</a></li><li><a href='/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis' style='color:#585F69;font-size:14px'>CLIA Integration Site Analysis <sup style="color:#826ec3; font-weight: bold;">NEW</sup></a></li></ul></div></div></div></li></ul></li><li class='dropdown'><a href='/en/Public/Research-Areas' data-toggle='dropdown' class='dropdown-toggle' style='color:white;height:52px;top:47px;padding-top:0;background-color:transparent;font-size:17px;font-weight:bold;'>RESEARCH AREAS</a><ul class='dropdown-menu'><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/AAV' style='font-size:17px'>AAV Services</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' href='/en/Public/Research-Areas/Agriculture-Food-Science' style='font-size:17px'>Agriculture and Food Science</a><ul class='dropdown-menu'></ul></li><li class='dropdown'><a class='GColor' 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padding: 0; } .ssdna-new-container { background: #f0f0f0; padding: 30px 0; } .ssdna-new-container h1 { margin: 0; color: #000000; } .ssdna-new-content p { color: #000000; } .ssdna-new-content { width: 1170px; margin: 0 auto; } .ssdna-content-box { display: inline-block; vertical-align: middle; /*width: 50%;*/ } #ssdna-content-box-left { width: 60%; text-align: left; padding: 30px 0; } #ssdna-content-box-right { width: 39%; text-align: right; } .ssdna-content-box img { width: 400px; } .ssdna-content-box button { border: none; border-radius: 4px; background: #826ec3; padding: 10px 20px; color: #fff; font-size: 15px; letter-spacing: 1px; } .gs-content-box button:hover { background: #6965a1; } @media only screen and (max-width: 768px) { .headline-center { padding-top: 0; } .ssdna-new-container { text-align: center; margin-top: 0; } .ssdna-new-content { width: 90%; margin: 0 auto; } .ssdna-new-content { display: inline-block; } #ssdna-content-box-right, #ssdna-content-box-left { width: 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Our ssDNA Synthesis fragments are derived from clonally purified double-stranded DNA (dsDNA), producing the highest quality results possible.</p> <p>Recent research in CRISPR/Cas9 technology has shown that the use of long ssDNA donor templates greatly enhances the efficiency of homology-directed repair (HDR) enabling researchers to optimize the process of efficiently generating transgenic animal models and cell lines. As the leader in complex gene sequences, you can trust GENEWIZ from Azenta Life Sciences for sequence-verified ssDNA synthesis for CRISPR gene knock-in, in-vitro transcription, and much more.</p> <p> </p> <a href="https://clims4.genewiz.com/CustomerHome/Index?lobId=2" target="_blank"><button>Place an Order</button></a> </div> <div class="ssdna-content-box" id="ssdna-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/ssdna-50off" target="_blank"><img alt="" src="/-/media/Images/Services/GS/photos/ssDNA-50-Off.ashx?la=en&hash=CB0FDBD30C41A23E4D368C31D359D2DEC545D9BF" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>What is the purpose of ssDNA?</h2> <p>ssDNA, or single-stranded DNA, is a nucleotide sequence involved in DNA replication and DNA repair.</p> <p> </p> <h2>What is the difference between dsDNA and ssDNA?</h2> <p>dsDNA stands for double-stranded DNA, which is two complimentary DNA strands forming a helical shape bound by hydrogen bonds. ssDNA stands for single-stranded DNA, and has a lower stability compared to dsDNA. Due to its double-stranded nature and strong hydrogen bond linkage, dsDNA is resistant to formaldehyde (unlike ssDNA).</p> <p> </p> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body { text-align: center; display: inline-block; } .banner-2 { width: 1170px; background-image: url('/-/media/Images/13064-D_Lagator-Customer-Webinar_WebBanFull-Med_Desktop-BG.ashx'); background-size: cover; text-align: center; display: flex; padding: 10px; } .banner img { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner-left { width: 40%; padding: 20px; } #banner-right-2 { width: 80%; padding: 10px; } #banner-right-2 h1 { text-transform: none; letter-spacing: 1px; line-height: 30px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 30px; margin: 0; margin-bottom: 5px; color: #FFFFFF } #banner-right-2 h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; 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font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 15px; color: #FFFFFF } #banner-left img { width: auto; height: auto; } #banner-right img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner-left img { width: 60%; } #banner-right button { font-size: 14px; } } </style> <div class="body"> <div class="banner-2"> <div id="banner-left"> <img alt="" src="-/media/.ashx" /></div> <div id="banner-right-2"> <div id="banner-right h1"> <h3>WEBINAR</h3> <h1>Understanding biological mechanisms to better predict the evolution of antibiotic resistance</h1> <div id="banner-right h3"> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/webinar/biological-mechanisms-predict-evolution-antibiotic-resistance?utm_campaign=2022-06 GEN NGS - New Customer Growth&utm_medium=web&utm_source=banner&utm_medium=Single%Stranded%DNA%Synthesis&__hstc=251210969.ee677d8374177d47808d4f65f2e7a318.1652957843001.1665408131811.1665479132940.197&__hssc=251210969.25.1665479132940&__hsfp=1345237511" target="_blank"><button>Watch Now</button></a> </div> </div> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>ssDNA Synthesis Applications</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Antibody Discovery:</strong> Engineer customized cell lines or transgenic mouse models to study in vivo immune responses </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Food technology:</strong> Modify genomes of agricultural crops to study pathogenic resistance for improved food security </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Cancer Biology:</strong> Generate CRISPR gene insertions to study oncogene function for targeted therapeutics </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Biofuels:</strong> Precise genome editing to optimize metabolic pathways for biofuel production </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .ssdna-container { background-color: #f0f0f0; padding: 30px 0; } .ssdna-content { width: 1170px; margin: 0 auto; } .workflow-image { display: inline-block; width: 48%; } .workflow-image img { width: 100%; } @media only screen and (max-width: 1024px) { .workflow-image { width: 100%; text-align: center; } .workflow-image img { width: 90%; margin: 0 auto; } } @media only screen and (max-width: 1170px) { .ssdna-content { width: 90%; margin: 0 auto; } } </style> <div class="ssdna-container"> <div class="ssdna-content"> <h2>CRISPR Gene Knock-In Workflow</h2> <p>Double-stranded breaks are generated through <a href="/Public/Research-Areas/Genome-Editing-and-Engineering" target="_blank">CRISPR/Cas9 editing</a>, then repaired by the endogenous cellular pathways of non-homologous end joining (NHEJ) and HDR. While the HDR pathway has consistently proven successful in copying genetic information via homologous recombination, insertion of exogenous genetic material is a challenge due to the inherent inefficiencies of HDR. Double-stranded DNA has historically been the template of choice for gene insertions, but recent research has shown the superiority of ssODNs as a donor template for HDR. Offering much higher efficiency to insert long sequences with shorter homology arms, ssDNA has become the preferred donor template for this process. Azenta now offers the longest (up to 10,000 nt) ssDNA fragments on the market, allowing insertion of long sequences with high efficiency and reduced cellular toxicity or off-target integration compared to dsDNA donors.</p> <div class="workflow-container"> <div class="workflow-image"> <img alt="" src="/-/media/Images/2xCRISPRMediatedGeneKnockInWorkflow112x1.ashx?la=en&hash=EC479FCA3D8997AC81A473DF1AA49BF8BD64569F" /> <p><strong>Figure 1.1.</strong> Guide RNA forms a complex with Cas9 directing enzyme to cleave target DNA resulting in a double-stranded break (DSB).</p> </div> <div class="workflow-image"> <img alt="" src="/-/media/Images/2xCRISPRMediatedGeneKnockInWorkflow122x1.ashx?la=en&hash=BA6A45C72765120CAB31C9A47A16C3DA1FBD58F5" /> <p><strong>Figure 1.2.</strong> Homology directed repair after DSB in the presence of a ssDNA donor template results in precise gene knock-in.</p> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <div class="headline-center"> <h2>ssDNA Advantages for CRISPR Gene Editing</h2> </div> <div class="headline-left"> </div> <div class="row category" style="display: inline-block;"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></em></div> <div class="content-boxes-in-v3"> <strong>Lower cellular toxicity</strong> compared to dsDNA after cellular delivery </div> </div> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></em></div> <div class="content-boxes-in-v3"> <strong>Low off-target integration</strong> for more reliable gene knock-ins </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></em></div> <div class="content-boxes-in-v3"> <strong>High specificity</strong> knock-in templates for homology directed repair </div> </div> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></em></div> <div class="content-boxes-in-v3" style="text-align: left;"> <strong>High efficiency</strong> donors for targeted insertions and gene replacements</div> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <center></center> <div class="container"> <div class="headline-center"> <h2>Fastest and Longest ssDNA Available</h2> </div> <table class="table table-responsive table-bordered" style="background-color: #ffffff; font-family: 'Open Sans', sans-serif; font-size: 14px;"> <tbody class="panel panel-purple"> <tr class="panel-heading"> <td>Length</td> <td>Available Yield</td> <td>Turnaround Time</td> </tr> <tr> <td>151-500 nt</td> <td>2, 3, 6, 10, 20, or 40 µg</td> <td>Starting at 10 Business Days</td> </tr> <tr> <td>501 - 2,000 nt</td> <td rowspan="2" style="vertical-align: middle;">3, 6, 10, 20, or 40 µg</td> <td>Starting at 15 Business Days</td> </tr> <tr> <td>2,001 - 8,000 nt</td> <td>Starting at 20 Business Days</td> </tr> </tbody> </table> </div> <p>Please note: 50% off complementary sequence for every order. Custom discount available for customer-supplied dsDNA. </p> <p>Email <a href="mailto: pm@azenta.com">pm@azenta.com</a> to submit custom inquiries for orders >8,000 nt.</p> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Single-Stranded DNA Features & Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Ph.D.-level consultation and support –</strong> Our dedicated Project Management team will tailor the order to your exact specifications and support your project from start to finish. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Advanced capabilities –</strong> Standard ssDNA synthesis service ranging from 150 nt to 8000 nt in length, with difficult stretches, like highly repetitive, AT- or GC-rich DNA. For ssDNA over 8000 nt in length, please contact <a href="mailto:PM@azenta.com">PM@azenta.com</a> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>AAV control applications – </strong>Synthesize ITR at each end of transgene. For the application such as AAV NGS library control, AAV qPCR control or AAV DNA ladder control. </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Quality control –</strong> Stringent quality control process with a 100% sequence accuracy guarantee. Residue rate of dsDNA lower than 0.5%.<br /> <br /> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Rapid delivery –</strong> Industry-leading turnaround time to keep your project running on time. </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> <strong>Yield flexibility –</strong> Choose 2, 3, 6, 10, 20, or 40 µg of lyophilized fragments.<br> <br> <br> </div> </div> </div> </div> </div> </div><div class="headline-center Rich-Text-Edit-Area"> <style> @media (max-width: 768px){ .service .desc { float:left; } </style> <div class="container no-padding text-left"> <div class="row row-centered margin-top-40 margin-bottom-40"> <div class="col-md-12 col-centered"> <div class="service"><span class="service-icon"><img alt="Case Study" src="/-/media/Images/13064-D_Lagator-Customer-Webinar_TechResource-Thumb.ashx?h=160&w=220&la=en&hash=4C5A728A0AA1CBF7B77B779F8EE170DFA2964413" style="height: 160px; width: 220px;" /></span> <div class="desc text-left"> <h4>Webinar | Understanding biological mechanisms to better predict the evolution of antibiotic resistance</h4> <p>The rapid and wide-spread evolution of antibiotic resistance is threatening global health. In this webinar, presented by Dr. Mato Lagator, Wellcome Trust, Royal Society Sir Henry Dale Fellow, University of Manchester, you will learn about different approaches to study evolutionary processes that underpin the emergence of resistance. Special focus is given on how these methods can be utilized to improve drug development and longevity.</p> <em class="fa icon fa-download icon-md"></em><a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/webinar/biological-mechanisms-predict-evolution-antibiotic-resistance?utm_campaign=2022-06 GEN NGS - New Customer Growth&utm_medium=web&utm_source=thumbnail&utm_medium=Single%Stranded%DNA%Synthesis" target="_blank"> <strong>Watch Now</strong></a></div> <div class="desc text-left"> </div> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style> .deliverables-container { padding: 40px 0; } </style> <div class="deliverables-container"> <h2>ssDNA Deliverables</h2> <ul style="display:inline-block;"> <li style="text-align: left;">Sequence verified</li> <li style="text-align: left;">2, 3, 6, 10, 20, or 40 µg lyophilized DNA</li> <li style="text-align: left;">Certificate of Analysis (COA) including gel image, sequence trace data with alignment, and sequence files</li> </ul> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style> .qc-container { padding: 40px 0; } </style> <div class="qc-container"> <h2>ssDNA Quality Control</h2> <ul style="display:inline-block"> <li style="text-align: left;">Sequence confirmation via Sanger sequencing</li> <li style="text-align: left;">Size verification by gel electrophoresis</li> <li style="text-align: left;">S1 nuclease digestion test</li> </ul> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style> .publications-container { padding: 60px 0; width: 1170px; margin: 0 auto; } #related-publications { padding-top: 160px; margin-top: -160px; } .publications-container li { font-size: 16px; } .publications-container li a { color: #0067B1; } @media only screen and (max-width: 1170px) { .publications-container { width: 90%; } } </style> <div class="publications-container" id="related-publications"> <h2>RELATED PUBLICATIONS</h2> <ol style="display:inline-block;"> <li style="text-align: left;">Li H, Beckman KA, Pessino V, et al. Design and specificity of long ssDNA donors for CRISPR-based knock-in. bioRxiv 178905. doi: <a href="https://www.biorxiv.org/content/early/2017/08/21/178905" target="_blank">10.1101/178905</a>.</li> <li style="text-align: left;">Mason DM, Weber CR, Parola C, et al. High-throughput antibody engineering in mammalian cells by CRISPR/Cas9-mediated homology-directed mutagenesis. <a href="https://www.ncbi.nlm.nih.gov/pubmed/29931269" target="_blank">Nucleic Acids Res.</a> (2018);46(14):7436-7449. doi: <a href="https://academic.oup.com/nar/article/46/14/7436/5042025" target="_blank">10.1093/nar/gky550</a></li> <li style="text-align: left;">Miura H, Quadros RM, Gurumurthy CB, et al. Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors. Nat Protoc. (2018) 13(1):195-215. doi: <a href="https://www.nature.com/articles/nprot.2017.153" target="_blank">10.1038/nprot.2017.153</a></li> <li style="text-align: left;">Roth TL, Puig-Saus, C, Yu R, et al. Reprogramming human T cell function and specificity with non-viral genome targeting. Nature 559. 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