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Shotgun Sequencing
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<li>Shotgun Sequencing</li> </ol> </nav> <div class="breadcrumb-main show-for-small-only hide"> <div class="crumb-parent"> <div class="link-block collapsed"> <a href="/">Home</a> </div> <div class="link-block collapsed"> <a href="/about-genomics">About Genomics</a> </div> <div class="link-block collapsed"> <a href="/About-Genomics/Educational-Resources">Educational Resources</a> </div> </div> <div class="link-block current-block "> <div class="current-page remove-padding"> <a href="/node/41621">Talking Glossary of Genomic and Genetic Terms</a></div> <span class="drop-arrow-container"> <i class="fa fa-chevron-down"></i> </span> </div> </div> </div> </div> </div> </div> <div id="block-languageswitch"> <div class="grid-container language-switch"><a href="/es/genetics-glossary/Secuenciacion-shotgun">En Espa帽ol</a></div> </div> </header> <main id="main" role="main"> <a id="main-content" tabindex="-1"></a> <img src="/themes/custom/nhgri/assets/global/NHGRI_grey_teal.svg" class="logo-print-only" alt="NHGRI logo" /> <div class="region region-content"> <div data-drupal-messages-fallback class="hidden"></div><div id="block-nhgri-content"> <article data-history-node-id="8576" class="node node--type-talking-glossary node--promoted node--view-mode-full nhgri_custom"> <div class="node__content"> <style> @import url("/themes/custom/nhgri/templates/paragraphs/talking-glossary/tg.css"); </style> <div class="tg-details"> <div class="tg-head"> <div class="tg-background"> <div class="bg-img" > <img src="/sites/default/files/media/images/tg/Shotgun-sequencing_hero.png" alt="Shotgun-sequencing_hero"> </div> </div> <div class="tg-box"> <div class="tg-title"> <div class="eyebrow"> </div> <div class="tg-title-box"> <h1>鈥婼hotgun Sequencing</h1> </div> <span class="tg-pro"></span> <span class="tg-date">updated: November 27, 2024</span> </div> </div> </div> <div class="tg-contents"> <div class="intro tg-box"> <div class="intro-title"> <h2> Definition </h2> </div> <div class="intro-content dm"> <audio preload="auto" controls> <source src="/sites/default/files/media/audio/2022-04/Shotgun_Sequencing_definition_Green_Eric.mp3"> </audio> <p class="dm en"> Shotgun sequencing is a laboratory technique for determining the DNA sequence of an organism鈥檚 genome. The method involves randomly breaking up the genome into small DNA fragments that are sequenced individually. A computer program looks for overlaps in the DNA sequences, using them to reassemble the fragments in their correct order to reconstitute the genome. </p> <div class="tg-img"> <span id="tg-max"> <svg id="icon" xmlns="http://www.w3.org/2000/svg" width="32" height="32" viewBox="0 0 32 32"> <defs> <style> .cls-1 { fill: none; } </style> </defs> <polygon class="tg-btn-white tg-max" points="20 2 20 4 26.586 4 18 12.582 19.414 14 28 5.414 28 12 30 12 30 2 20 2"/> <polygon class="tg-btn-white tg-max" points="14 19.416 12.592 18 4 26.586 4 20 2 20 2 30 12 30 12 28 5.414 28 14 19.416"/> <polygon class="tg-btn-white tg-min" points="4 18 4 20 10.586 20 2 28.582 3.414 30 12 21.414 12 28 14 28 14 18 4 18"/> <polygon class="tg-btn-white tg-min" points="30 3.416 28.592 2 20 10.586 20 4 18 4 18 14 28 14 28 12 21.414 12 30 3.416"/> <rect id="_Transparent_Rectangle_" data-name="<Transparent Rectangle>" class="cls-1" width="32" height="32" transform="translate(32 32) rotate(-180)"/> </svg> </span> <img class="tg-image" src="/sites/default/files/media/images/tg/Shotgun-sequencing.jpg" alt=" Shotgun-sequencing"> </div> <br/> <br/> </div> </div> <div class="narration tg-box line-t"> <div class="narration-title"> <h2>Narration</h2> </div> <div class="narration-contents"> <audio preload="auto" controls> <source src="/sites/default/files/media/audio/tg/Shotgun_Sequencing_transcription_Green_Eric.mp3"> </audio> <p class="dm"> Imagine taking a page in a book, making hundreds of copies of it, taking those copies and putting them in a paper shredder that magically creates strips of paper that contain various bits and pieces of the original sentences on the starting page. Then imagine reading each of those strips containing the bits and pieces of the sentences, looking for overlapping words and phrases, and eventually being able to reconstruct the entire text on the starting page by having read enough of those shreds of paper. In essence, that is shotgun DNA sequencing. Instead of a page of a book, a scientist starts with a piece of clone DNA or even an entire genome. The DNA is broken apart and many many many many many sequence reads are generated from the DNA pieces. All of those data are analyzed by a computer program that looks for overlapping stretches of sequence and eventually puts that DNA puzzle back together. </p> <div class="tg-cast"> <div class="tg-user"> <img src="/sites/default/files/media/images/2024-02/green_eric.jpg" alt="Eric Green"> </div> <div class="tg-user-details"> <a href="/staff/Eric-D-Green-MD-PhD" class="name"> Eric Green, M.D., Ph.D.</a> <p>Director</p> <p>Office of the Director</p> </div> </div> </div> </div> </div> <div class="tg-tail "> <div class="tg-box line-t"> <div class="tg-search" style="margin-top: 30px;"> <h2>Search</h2> <div class="eyebrow"></div> <div class="tg-search-content"> <div id="sg"> <div class="clear-search ui-widget"> <input class="glossary-search gtsearch " id="gsearch" type="text" placeholder="type to search" tabindex="1"> </input> <div class="tg-search-list"></div> </div> </div> <div> <span class="tg-back"> <a href="/genetics-glossary">Back to Glossary</a> </span> </div> </div> </div> <div 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