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<!doctype html> <html lang="en"> <head> <meta charset="utf-8"> <title>Construction of a Novel Polyphyllin I-regulated PANoptosis Prognosis Model by WGCNA and Machine Learning Algorithms in Non-small Cell Lung Cancer-International Journal of Clinical and Experimental Medicine Research-Hill Publishing Group</title> <meta content="width=device-width, initial-scale=1" name="viewport"> <meta content="Construction of a Novel Polyphyllin I-regulated PANoptosis Prognosis Model by WGCNA and Machine Learning Algorithms in Non-small Cell Lung Cancer-International Journal of Clinical and Experimental Medicine Research-Hill Publishing Group" name="description"> <meta content="International Journal of Clinical and Experimental Medicine Research-Hill Publishing Group" name="keywords"> <link href="/css/hillpub.css" type="text/css" rel="stylesheet"> <script type="text/ecmascript" src="/js/tab.js"></script> <script type="text/javascript" src="/js/jquery1.3.2.js"></script> <script type="text/javascript"> $(document).ready(function () { $('.menutitle').click(function () { if ($(this).siblings('ul').css('display') == 'none') { $(this).parent('li').siblings('li').removeClass('active'); $(this).addClass('active'); $(this).siblings('ul').slideDown(100).children('li'); if ($(this).parents('li').siblings('li').children('ul').css('display') == 'block') { $(this).parents('li').siblings('li').children('ul').parent('li').children('a').removeClass('active'); $(this).parents('li').siblings('li').children('ul').slideUp(100); } } else { $(this).removeClass('active'); $(this).siblings('ul').slideUp(100); $(this).siblings('ul').children('li').children('ul').parent('li').children('a').addClass('active'); $(this).siblings('ul').children('li').children('ul').slideUp(100); $(this).siblings('ul').children('li').children('a').removeClass('active'); } }) }); </script> <script type="text/javascript"> var browser = { versions: function () { var u = navigator.userAgent, app = navigator.appVersion; return { trident: u.indexOf('Trident') > -1, presto: u.indexOf('Presto') > -1, webKit: u.indexOf('AppleWebKit') > -1, gecko: u.indexOf('Gecko') > -1 && u.indexOf('KHTML') == -1, mobile: !!u.match(/AppleWebKit.*Mobile.*/), ios: !!u.match(/\(i[^;]+;( U;)? 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class="art-authors"> <p style="padding-bottom: 15px; margin-bottom: 0px; line-height: 22px; outline: none;"><font color="#000000" face="Poppins"><span style="font-size: 14px;">Kai Tan</span><sup><span style="font-size: 14px;">1</span></sup><span style="font-size: 14px;">, Changhui Zhang</span><sup><span style="font-size: 14px;">1</span></sup><span style="font-size: 14px;">, Zuomei He</span><sup><span style="font-size: 14px;">2,3</span></sup><span style="font-size: 14px;">, Puhua Zeng</span><sup><span style="font-size: 14px;">2,3,*</span></sup></font></p><p style="padding-bottom: 15px; margin-bottom: 0px; line-height: 22px; outline: none;"><font color="#000000" face="Poppins"><sup><span style="font-size: 14px;">1</span></sup><span style="font-size: 14px;">Hunan University of Chinese Medicine, Changsha 410208, Hunan, China.</span></font></p><p style="padding-bottom: 15px; margin-bottom: 0px; line-height: 22px; outline: none;"><font color="#000000" face="Poppins"><sup><span style="font-size: 14px;">2</span></sup><span style="font-size: 14px;">Cancer Research Institute of Hunan Academy of Traditional Chinese Medicine, Changsha 410006, Hunan, China.</span></font></p><p style="padding-bottom: 15px; margin-bottom: 0px; line-height: 22px; outline: none;"><font color="#000000" face="Poppins"><sup><span style="font-size: 14px;">3</span></sup><span style="font-size: 14px;">Hunan Provincial Hospital of Integrated Traditional Chinese and Western Medicine, Changsha 410006, Hunan, China.</span></font></p><p style="padding-bottom: 15px; margin-bottom: 0px; line-height: 22px; outline: none;"><strong style="color: rgb(0, 0, 0); font-family: Arial, sans-serif, "Hiragino Sans GB", "Microsoft YaHei", "WenQuanYi Micro Hei" !important; font-size: 16px;"><span style="padding-left: 4px; outline: none; font-family: Poppins; font-size: 14px;">*Corresponding author: </span></strong><font color="#000000" face="Poppins"><span style="font-size: 14px;">Puhua Zeng</span></font></p> </div> <div class="art-affiliations"> </div> <div class="art-pubhistory"> Published: October 30,2024 </div> <ul class="wzzhbox1"> <li id="left" class="preAfter" style="display:block;"> <a href="/ArticleDetails/3766"> <span>Previous article in journal</span> </a> </li> <li id="right" class="next" style="display:block;"> <a href="/ArticleDetails/3966"> <span>Next article in journal</span> </a> </li> </ul> <div class="abt"> <h2>Abstract</h2> <p><p><b>Objective:</b> To predict Polyphyllin I (PPI) targets, analyze their effects on non-small cell lung cancer (NSCLC) patients, develop a PANoptosis-based prognostic model, and identify potential molecular markers that can be used as PPI targets for the treatment of NSCLC. <b>Methods:</b> We predicted the targets of PPI and analyzed the main effect of PPI targets in NSCLC patients by principal component analysis (PCA). The PANoptosis genes associated with PPI and immune infiltration were explored using the weighted gene co-expression network analysis (WGCNA) algorithm. Then, the least absolute shrinkage and selection operator (LASSO), random forest (RF), support vector machine-recursive feature elimination (SVM-RFE) and extreme gradient boosting (XGBoost) algorithms were used to screen the feature genes, and the prognostic model and clinical nomogram were constructed. Finally, the differences in the immune characteristics and mutations of patients with different risk scores were compared, and the expression of the modeled genes at the gene and protein levels was explored using single-cell sequencing and immunohistochemical techniques. <b>Results: </b>We obtained the PPI effective index (PEI) using the NSCLC data of the training and the validation sets. Meanwhile, both datasets identified strong interaction relationships between PPI and PANoptosis and screened out PPI-induced PANoptosis genes (PPGs). Then, the PPG_score model and clinical nomogram were successfully constructed. Finally, we found that PPG_score patients had different immune responses, and independent predictors were highly expressed in lung cancer tissues and fibroblasts. <b>Conclusion:</b> In this study, a PANoptosis prognostic model regulated by PPI has a guiding role for both immunotherapy and mutations, and the screened independent prognostic factors, <i>IRF1</i>, <i>PTGIS</i>, and <i>G0S2</i>, may become potential molecular markers and PPI targets for NSCLC patients.</p></p> </div> <div class="re" > <h2>References</h2> <p>[1]<span style="white-space:pre"> </span>SUNG H, FERLAY J, SIEGEL R L, et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA: a cancer journal for clinicians. 2021;71(3):209-49.</p><p>[2]<span style="white-space:pre"> </span>WANG M, HERBST R S, BOSHOFF C. Toward personalized treatment approaches for non-small-cell lung cancer. Nature medicine. 2021;27(8):1345-56.</p><p>[3]<span style="white-space:pre"> </span>LANCASTER H L, HEUVELMANS M A, OUDKERK M. Low-dose computed tomography lung cancer screening: Clinical evidence and implementation research. Journal of internal medicine. 2022;292(1):68-80.</p><p>[4]<span style="white-space:pre"> </span>CAMIDGE D R, DOEBELE R C, KERR K M. Comparing and contrasting predictive biomarkers for immunotherapy and targeted therapy of NSCLC. Nature reviews Clinical oncology. 2019;16(6):341-55.</p><p>[5]<span style="white-space:pre"> </span>LI Z, FEIYUE Z, GAOFENG L. Traditional Chinese medicine and lung cancer--From theory to practice. Biomedicine & pharma-cotherapy = Biomedecine & pharmacotherapie. 2021;137:111381.</p><p>[6]<span style="white-space:pre"> </span>WANG X, HOU L, CUI M, et al. The traditional Chinese medicine and non-small cell lung cancer: from a gut microbiome perspective. Frontiers in cellular and infection microbiology. 2023;13:1151557.</p><p>[7]<span style="white-space:pre"> </span>JIANG R, HU C, LI Q, et al. Sodium new houttuyfonate suppresses metastasis in NSCLC cells through the Linc00668/miR-147a/slug axis. Journal of experimental & clinical cancer research: CR. 2019;38(1):155.</p><p>[8]<span style="white-space:pre"> </span>XU L, MENG X, XU N, et al. Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth. Cell death & disease. 2018;9(3):262.</p><p>[9]<span style="white-space:pre"> </span>YAO C, SU L, ZHANG F, et al. Thevebioside, the active ingredient of traditional Chinese medicine, promotes ubiquitin-mediated SRC-3 degradation to induce NSCLC cells apoptosis. Cancer letters. 2020;493:167-77.</p><p>[10]<span style="white-space:pre"> </span>COMMISSION C P. Pharmacopoeia of People’s Republic of China. Beijing: China Medical Science Press. 2020:271-2.</p><p>[11]<span style="white-space:pre"> </span>WANG K, CHEN Q, SHAO Y, et al. Anticancer activities of TCM and their active components against tumor metastasis. Bio-medicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2021;133:111044.</p><p>[12]<span style="white-space:pre"> </span>LAI L, SHEN Q, WANG Y, et al. Polyphyllin I reverses the resistance of osimertinib in non-small cell lung cancer cell through regulation of PI3K/Akt signaling. Toxicology and applied pharmacology. 2021;419:115518.</p><p>[13]<span style="white-space:pre"> </span>LI H S, XU Y. Inhibition of EZH2 via the STAT3/HOTAIR signalling axis contributes to cell cycle arrest and apoptosis induced by polyphyllin I in human non-small cell lung cancer cells. Steroids. 2020;164:108729.</p><p>[14]<span style="white-space:pre"> </span>FENG F F, CHENG P, SUN C, et al. Inhibitory effects of polyphyllins I and VII on human cisplatin-resistant NSCLC via p53 upregulation and CIP2A/AKT/mTOR signaling axis inhibition. Chinese journal of natural medicines. 2019;17(10):768-77.</p><p>[15]<span style="white-space:pre"> </span>YANG Q, CHEN W, XU Y, et al. Polyphyllin I modulates MALAT1/STAT3 signaling to induce apoptosis in gefitinib-resistant non-small cell lung cancer. Toxicology and applied pharmacology. 2018;356:1-7.</p><p>[16]<span style="white-space:pre"> </span>LIU Y, ZHANG W, ZHOU H, et al. Steroidal saponins PPI/CCRIS/PSV induce cell death in pancreatic cancer cell through GSDME-dependent pyroptosis. Biochemical and biophysical research communications. 2023;673:51-8.</p><p>[17]<span style="white-space:pre"> </span>LUO Q, YANG D, QI Q, et al. Role of the Death Receptor and Endoplasmic Reticulum Stress Signaling Pathways in Polyphyllin I-Regulated Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells. BioMed research international. 2018;2018:5241941.</p><p>[18]<span style="white-space:pre"> </span>KURIAKOSE T, MAN S M, MALIREDDI R K, et al. ZBP1/DAI is an innate sensor of influenza virus triggering the NLRP3 inflammasome and programmed cell death pathways. Science immunology. 2016;1(2).</p><p>[19]<span style="white-space:pre"> </span>KESAVARDHANA S, KURIAKOSE T, GUY C S, et al. ZBP1/DAI ubiquitination and sensing of influenza vRNPs activate programmed cell death. The Journal of experimental medicine. 2017;214(8):2217-29.</p><p>[20]<span style="white-space:pre"> </span>MALIREDDI R K S, GURUNG P, MAVULURI J, et al. TAK1 restricts spontaneous NLRP3 activation and cell death to control myeloid proliferation. The Journal of experimental medicine. 2018;215(4):1023-34.</p><p>[21]<span style="white-space:pre"> </span>MALIREDDI R K S, KESAVARDHANA S, KANNEGANTI T D. ZBP1 and TAK1: Master Regulators of NLRP3 Inflam-masome/Pyroptosis, Apoptosis, and Necroptosis (PAN-optosis). Frontiers in cellular and infection microbiology. 2019;9:406.</p><p>[22]<span style="white-space:pre"> </span>MALIREDDI R K S, GURUNG P, KESAVARDHANA S, et al. Innate immune priming in the absence of TAK1 drives RIPK1 kinase activity-independent pyroptosis, apoptosis, necroptosis, and inflammatory disease. The Journal of experimental medicine. 2020;217(3).</p><p>[23]<span style="white-space:pre"> </span>CHRISTGEN S, ZHENG M, KESAVARDHANA S, et al. Identification of the PANoptosome: A Molecular Platform Triggering Pyroptosis, Apoptosis, and Necroptosis (PANoptosis). Frontiers in cellular and infection microbiology. 2020;10:237.</p><p>[24]<span style="white-space:pre"> </span>MALIREDDI R K S, KARKI R, SUNDARAM B, et al. Inflammatory Cell Death, PANoptosis, Mediated by Cytokines in Di-verse Cancer Lineages Inhibits Tumor Growth. ImmunoHorizons. 2021;5(7):568-80.</p><p>[25]<span style="white-space:pre"> </span>KARKI R, SUNDARAM B, SHARMA B R, et al. ADAR1 restricts ZBP1-mediated immune response and PANoptosis to promote tumorigenesis. Cell reports. 2021;37(3):109858.</p><p>[26]<span style="white-space:pre"> </span>KARKI R, SHARMA B R, LEE E, et al. Interferon regulatory factor 1 regulates PANoptosis to prevent colorectal cancer. JCI insight. 2020;5(12).</p><p>[27]<span style="white-space:pre"> </span>WEI S, CHEN Z, LING X, et al. Comprehensive analysis illustrating the role of PANoptosis-related genes in lung cancer based on bioinformatic algorithms and experiments. Frontiers in pharmacology. 2023;14:1115221.</p><p>[28]<span style="white-space:pre"> </span>LI Y, QI D, ZHU B, et al. Analysis of m6A RNA Methylation-Related Genes in Liver Hepatocellular Carcinoma and Their Correlation with Survival. International journal of molecular sciences. 2021;22(3).</p><p>[29]<span style="white-space:pre"> </span>GIANNINI H M, GINESTRA J C, CHIVERS C, et al. A Machine Learning Algorithm to Predict Severe Sepsis and Septic Shock: Development, Implementation, and Impact on Clinical Practice. Critical care medicine. 2019;47(11):1485-92.</p><p>[30]<span style="white-space:pre"> </span>SADOZAI H, ACHARJEE A, EPPENBERGER-CASTORI S, et al. Distinct Stromal and Immune Features Collectively Contribute to Long-Term Survival in Pancreatic Cancer. Frontiers in immunology. 2021;12:643529.</p><p>[31]<span style="white-space:pre"> </span>WANG Y, HUANG Z, XIAO Y, et al. The shared biomarkers and pathways of systemic lupus erythematosus and metabolic syndrome analyzed by bioinformatics combining machine learning algorithm and single-cell sequencing analysis. Frontiers in immunology. 2022;13:1015882.</p><p>[32]<span style="white-space:pre"> </span>CHEN T, GUESTRIN C. Xgboost: A scalable tree boosting system; proceedings of the Proceedings of the 22nd acm sigkdd international conference on knowledge discovery and data mining, F, 2016 [C].</p><p>[33]<span style="white-space:pre"> </span>SUN D, WANG J, HAN Y, et al. TISCH: a comprehensive web resource enabling interactive single-cell transcriptome visualization of tumor microenvironment. Nucleic acids research. 2021;49(D1):D1420-d30.</p><p>[34]<span style="white-space:pre"> </span>KIM N, KIM H K, LEE K, et al. Single-cell RNA sequencing demonstrates the molecular and cellular reprogramming of metastatic lung adenocarcinoma. Nature communications. 2020;11(1):2285.</p><p>[35]<span style="white-space:pre"> </span>MILLER M, HANNA N. Advances in systemic therapy for non-small cell lung cancer. BMJ (Clinical research ed). 2021;375:n2363.</p><p>[36]<span style="white-space:pre"> </span>TIAN Y, GONG G Y, MA L L, et al. Anti-cancer effects of Polyphyllin I: An update in 5 years. Chemico-biological interactions. 2020;316:108936.</p><p>[37]<span style="white-space:pre"> </span>WELCH C, SANTRA M K, EL-ASSAAD W, et al. Identification of a protein, G0S2, that lacks Bcl-2 homology domains and interacts with and antagonizes Bcl-2. Cancer research. 2009;69(17):6782-9.</p><p>[38]<span style="white-space:pre"> </span>XIONG T, LV X S, WU G J, et al. Single-Cell Sequencing Analysis and Multiple Machine Learning Methods Identified G0S2 and HPSE as Novel Biomarkers for Abdominal Aortic Aneurysm. Frontiers in immunology. 2022;13:907309.</p><p>[39]<span style="white-space:pre"> </span>KUSAKABE M, KUTOMI T, WATANABE K, et al. Identification of G0S2 as a gene frequently methylated in squamous lung cancer by combination of in silico and experimental approaches. International journal of cancer. 2010;126(8):1895-902.</p><p>[40]<span style="white-space:pre"> </span>ZAGANI R, EL-ASSAAD W, GAMACHE I, et al. Inhibition of adipose triglyceride lipase (ATGL) by the putative tumor suppressor G0S2 or a small molecule inhibitor attenuates the growth of cancer cells. Oncotarget. 2015;6(29):28282-95.</p><p>[41]<span style="white-space:pre"> </span>HATAE T, WADA M, YOKOYAMA C, et al. Prostacyclin-dependent apoptosis mediated by PPAR delta. The Journal of biological chemistry. 2001;276(49):46260-7.</p><p>[42]<span style="white-space:pre"> </span>PAN X Y, YANG Y, MENG H W, et al. DNA Methylation of PTGIS Enhances Hepatic Stellate Cells Activation and Liver Fibrogenesis. Frontiers in pharmacology. 2018;9:553.</p><p>[43]<span style="white-space:pre"> </span>LEI K, LIANG R, TAN B, et al. Effects of Lipid Metabolism-Related Genes PTGIS and HRASLS on Phenotype, Prognosis, and Tumor Immunity in Lung Squamous Cell Carcinoma. Oxidative medicine and cellular longevity. 2023;2023:6811625.</p><p>[44]<span style="white-space:pre"> </span>DAI D, CHEN B, FENG Y, et al. Prognostic value of prostaglandin I2 synthase and its correlation with tumor-infiltrating immune cells in lung cancer, ovarian cancer, and gastric cancer. Aging. 2020;12(10):9658-85.</p><p>[45]<span style="white-space:pre"> </span>LIANG X, WANG J, LIU Y, et al. Polymorphisms of COX/PEG2 pathway-related genes are associated with the risk of lung cancer: A case-control study in China. International immunopharmacology. 2022;108:108763.</p><p>[46]<span style="white-space:pre"> </span>MILOVIC-HOLM K, KRIEGHOFF E, JENSEN K, et al. FLASH links the CD95 signaling pathway to the cell nucleus and nuclear bodies. The EMBO journal. 2007;26(2):391-401.</p><p>[47]<span style="white-space:pre"> </span>MELENOTTE C, MEZOUAR S, BEN AMARA A, et al. A transcriptional signature associated with non-Hodgkin lymphoma in the blood of patients with Q fever. PloS one. 2019;14(6):e0217542.</p><p>[48]<span style="white-space:pre"> </span>DUAN Y, DU Y, MU Y, et al. Expression, prognostic value and mechanism of SP100 family in pancreatic adenocarcinoma. Aging. 2023;15(12):5569-91.</p><p>[49]<span style="white-space:pre"> </span>BERSCHEMINSKI J, BRUN J, SPEISEDER T, et al. Sp100A is a tumor suppressor that activates p53-dependent transcription and counteracts E1A/E1B-55K-mediated transformation. Oncogene. 2016;35(24):3178-89.</p><p>[50]<span style="white-space:pre"> </span>HELD-FEINDT J, HATTERMANN K, KNERLICH-LUKOSCHUS F, et al. SP100 reduces malignancy of human glioma cells. International journal of oncology. 2011;38(4):1023-30.</p><p>[51]<span style="white-space:pre"> </span>DONNER I, KATAINEN R, SIPILä L J, et al. Germline mutations in young non-smoking women with lung adenocarcinoma. Lung cancer (Amsterdam, Netherlands). 2018;122:76-82.</p><p>[52]<span style="white-space:pre"> </span>KARKI R, SHARMA B R, TULADHAR S, et al. Synergism of TNF-α and IFN-γ Triggers Inflammatory Cell Death, Tissue Damage, and Mortality in SARS-CoV-2 Infection and Cytokine Shock Syndromes. Cell. 2021;184(1):149-68.e17.</p><p>[53]<span style="white-space:pre"> </span>HUANG J X, WU Y C, CHENG Y Y, et al. IRF1 Negatively Regulates Oncogenic KPNA2 Expression Under Growth Stimulation and Hypoxia in Lung Cancer Cells. OncoTargets and therapy. 2019;12:11475-86.</p><p>[54]<span style="white-space:pre"> </span>JIN J, YU G. Hypoxic lung cancer cell-derived exosomal miR-21 mediates macrophage M2 polarization and promotes cancer cell proliferation through targeting IRF1. World journal of surgical oncology. 2022;20(1):241.</p><p>[55]<span style="white-space:pre"> </span>ZHANG L, CHENG T, YANG H, et al. Interferon regulatory factor-1 regulates cisplatin-induced apoptosis and autophagy in A549 lung cancer cells. Medical oncology (Northwood, London, England). 2022;39(4):38.</p><p>[56]<span style="white-space:pre"> </span>WU M, TU J, HUANG J, et al. Exosomal IRF1-loaded rat adipose-derived stem cell sheet contributes to wound healing in the diabetic foot ulcers. Molecular medicine (Cambridge, Mass). 2023;29(1):60.</p><p>[57]<span style="white-space:pre"> </span>ORZALLI M H, SMITH A, JURADO K A, et al. An Antiviral Branch of the IL-1 Signaling Pathway Restricts Immune-Evasive Virus Replication. Molecular cell. 2018;71(5):825-40.e6.</p><p>[58]<span style="white-space:pre"> </span>WANG J, IKEDA R, CHE X F, et al. VEGF expression is augmented by hypoxia‑induced PGIS in human fibroblasts. International journal of oncology. 2013;43(3):746-54.</p><p>[59]<span style="white-space:pre"> </span>SOMMERFELD L, KNUTH I, FINKERNAGEL F, et al. Prostacyclin Released by Cancer-Associated Fibroblasts Promotes Immunosuppressive and Pro-Metastatic Macrophage Polarization in the Ovarian Cancer Microenvironment. Cancers. 2022;14(24).</p><p>[60]<span style="white-space:pre"> </span>YIM C Y, SEKULA D J, HEVER-JARDINE M P, et al. G0S2 Suppresses Oncogenic Transformation by Repressing a MYC-Regulated Transcriptional Program. Cancer research. 2016;76(5):1204-13.</p><p>[61]<span style="white-space:pre"> </span>CHOI H, LEE H, KIM T H, et al. G0/G1 switch gene 2 has a critical role in adipocyte differentiation. Cell death and differentiation. 2014;21(7):1071-80.</p> </div> <div class="ftex" id="fu"> <h2>How to cite this paper</h2> <p style="padding-bottom: 15px; margin-bottom: 0px; line-height: 22px; outline: none;"><font color="#000000" face="Poppins"><b><span style="font-size: 14px;">Construction of a Novel Polyphyllin I-regulated PANoptosis Prognosis Model by WGCNA and Machine Learning Algorithms in Non-small Cell Lung Cancer</span></b></font></p><p style="padding-bottom: 15px; margin-bottom: 0px; line-height: 22px; outline: none;"><b style="color: rgb(0, 0, 0); font-family: Arial, sans-serif, "Hiragino Sans GB", "Microsoft YaHei", "WenQuanYi Micro Hei"; font-size: 16px;"><span style="outline: none; font-family: Poppins; font-size: 14px;">How to cite this paper: </span></b><span style="color: rgb(0, 0, 0); font-family: Arial, sans-serif, "Hiragino Sans GB", "Microsoft YaHei", "WenQuanYi Micro Hei"; font-size: 16px; outline: none;"><span style="outline: none; font-family: Poppins; font-size: 14px;">Kai Tan, Changhui Zhang, Zuomei He, Puhua Zeng. (2024) </span></span><span style="outline: none;"><font color="#000000" face="Poppins" style=""><font style=""><span style="font-size: 14px;">Construction of a Novel Polyphyllin I-regulated PANoptosis Prognosis Model by WGCNA and Machine Learning Algorithms in Non-small Cell Lung Cancer</span><font color="#313131" face="Arial, Helvetica, sans-serif, 微软雅黑"><span style="font-size: 14px;">.</span></font></font><font color="#313131" face="Arial, Helvetica, sans-serif, 微软雅黑" style="color: rgb(49, 49, 49); font-family: Arial, Helvetica, sans-serif, 微软雅黑; font-size: 16px;"><span style="font-size: 14px;"> </span></font></font></span><i style="color: rgb(0, 0, 0); font-family: Arial, sans-serif, "Hiragino Sans GB", "Microsoft YaHei", "WenQuanYi Micro Hei"; font-size: 16px;"><span style="outline: none; font-family: Poppins; font-size: 14px;">International Journal of Clinical and Experimental Medicine Research</span></i><span style="color: rgb(0, 0, 0); font-family: Poppins; font-size: 14px; outline: none;"><span style="outline: none;"><span style="font-size: 14px;">, </span><b><span style="font-size: 14px;">8</span></b></span></span><span style="color: rgb(0, 0, 0); font-family: Poppins; font-size: 14px; outline: none;"><span style="outline: none; font-family: Arial, sans-serif, "Hiragino Sans GB", "Microsoft YaHei", "WenQuanYi Micro Hei" !important; font-size: 16px !important;"><span style="outline: none; font-family: Poppins; font-size: 14px;"><span style="font-size: 14px;">(</span><b><span style="font-size: 14px;">4</span></b></span></span></span><span style="color: rgb(0, 0, 0); font-family: Poppins; font-size: 14px; outline: none;">), 528-546.</span></p><p style="padding-bottom: 15px; margin-bottom: 0px; line-height: 22px; font-size: 16px; outline: none; font-family: Arial, sans-serif, "Hiragino Sans GB", "Microsoft YaHei", "WenQuanYi Micro Hei"; color: rgb(0, 0, 0);"><span style="outline: none; font-family: Poppins; font-size: 14px;">DOI: https://dx.doi.org/10.26855/ijcemr.2024.10.003</span></p> </div> </div>  </div>  </div>  <div class="footer"> <div class="foot_nav"><a href="/Index" title="Home">Home</a>　|　<a href="/Journals" title="Journals">Journals</a>　|　<a href="/Books" title="Books">Books</a>　|　<a href="/ForAuthors" title="For Authors and Reviewers">For Authors and Reviewers</a>　|　<a href="/OnlineSubmission" title="Online Submission">Online Submission</a>　|　<a href="ContactUs" title="Contact Us">Contact Us</a>　|　<a href="/AboutUs" title="About Us">About Us</a></div> <p>Copyright © 2023 Hill Publishing Group Inc. 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