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mTeSR™ Plus cGMP Pluripotent Stem Cell Maintenance Medium | STEMCELL Technologies
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<span> <span style="font-weight:700"> Now Available:</span> human induced pluripotent stem cell lines, <a href="/products/healthy-control-human-ipsc-line-female-scti003-a.html">SCTi003-A</a> (female), and <a href="/products/healthy-control-human-ipsc-line-male-scti004-a.html">SCTi004-A</a> (male), manufactured with mTeSR™ Plus. </span> </div> </div> </div><div class="product-info-main"><div class="product-add-form"> <form data-product-sku="100-0276_C" action="https://www.stemcell.com/checkout/cart/add/uenc/aHR0cHM6Ly93d3cuc3RlbWNlbGwuY29tL3Byb2R1Y3RzL210ZXNyLXBsdXMuaHRtbA~~/product/32119/" method="post" id="product_addtocart_form" class="product_addtocart_form" > <input type="hidden" name="product" value="32119" /> <input type="hidden" name="selected_configurable_option" value="" /> <input type="hidden" name="related_product" id="related-products-field" value="" /> <input name="form_key" type="hidden" value="KttIlSLJ19fpsnOP" /> <div class="product-options-bottom"> <div 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stabilized feeder-free maintenance medium for human ES and iPS cells","37457":"cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells"},"registration_forms":{"32129":"","37457":""}}, "gallerySwitchStrategy": "prepend" } } } </script> <script> require([ 'jquery' ], function($){ //<![CDATA[ $.extend(true, $, { calendarConfig: { dayNames: ["Sunday","Monday","Tuesday","Wednesday","Thursday","Friday","Saturday"], dayNamesMin: ["Sun","Mon","Tue","Wed","Thu","Fri","Sat"], monthNames: ["January","February","March","April","May","June","July","August","September","October","November","December"], monthNamesShort: ["Jan","Feb","Mar","Apr","May","Jun","Jul","Aug","Sep","Oct","Nov","Dec"], infoTitle: 'About\u0020the\u0020calendar', firstDay: 0, closeText: 'Close', currentText: 'Go\u0020Today', prevText: 'Previous', nextText: 'Next', weekHeader: 'WK', timeText: 'Time', hourText: 'Hour', minuteText: 'Minute', dateFormat: "D, d M yy", // $.datepicker.RFC_2822 showOn: 'button', showAnim: '', changeMonth: true, changeYear: true, buttonImageOnly: null, buttonImage: null, showButtonPanel: true, showWeek: true, timeFormat: '', showTime: false, showHour: false, showMinute: false } }); enUS = {"m":{"wide":["January","February","March","April","May","June","July","August","September","October","November","December"],"abbr":["Jan","Feb","Mar","Apr","May","Jun","Jul","Aug","Sep","Oct","Nov","Dec"]}}; // en_US locale reference //]]> });</script> </div> <div class="product-attributes" data-sku="100-0276" style="display:none;"> <div class="product-attribute"> <label> Size </label> <span>500 mL</span> </div> </div> <div class="product-attributes" data-sku="100-1130" style="display:none;"> <div class="product-attribute"> <label> Size </label> <span>1 L</span> </div> </div> </div> <div class="product attribute sku"> <strong class="type">Catalog #</strong> <div class="value selected-sku-for-product-availability" itemprop="sku"> (Select a product) </div> </div> <div class="product attribute overview option-overview"> <div class="value selected-sku-for-product-availability" itemprop="description"> cGMP, stabilized feeder-free maintenance medium for human ES and iPS cells </div> </div> <div class="product-availability for-pdp" data-for-product-type="configurable" data-for-area="pdp"> <i class="icon hidden fa"></i> <div class="label hidden"></div> </div> <div class="lead-time label hidden"></div> <script type="text/x-magento-init"> { "*": { "Stemcell_ProductAvailability/js/configurable-options-handler": {"checkmark_css":"fa-check checkmark","warning_css":"fa-warning warning","auto_select_product_id":null,"auto_select_product_sku":null,"list":{"by_id":[],"by_sku":[]},"lead_times":{"by_id":[],"by_sku":[]}} } } </script> <div class="box-tocart standard-product"> <div class="fieldset"> <div class="field configurable required qty-box" style="display: none;"> <label class="label">Quantity</label> </div> <div class="row" style="display: none;"> <div class="qty-column"> <div class="field qty"> <span class="fa fa-minus qty-minus"></span> <div class="control"> <input type="number" name="qty" id="qty" value="1" title="Qty" class="input-text qty" data-validate="{"required-number":true,"validate-item-quantity":{"maxAllowed":10000}}" /> </div> <span class="fa fa-plus qty-plus"></span> </div> </div> <div class="price-column"> </div> </div> <div class="actions"> <button type="submit" title="Add to Cart" class="action primary tocart" id="product-addtocart-button" style="display: none;"> <span>Add to Cart</span> </button> <a class="action primary request-pricing" data-id="32119" data-name="mTeSR™ Plus" data-href="#request_price_form"> Request Pricing </a> <div id="instant-purchase" data-bind="scope:'instant-purchase'"> <!-- ko template: getTemplate() --><!-- /ko --> </div> <script type="text/x-magento-init"> { "#instant-purchase": { "Magento_Ui/js/core/app": {"components":{"instant-purchase":{"component":"Magento_InstantPurchase\/js\/view\/instant-purchase","config":{"template":"Magento_InstantPurchase\/instant-purchase","buttonText":"Instant Purchase","purchaseUrl":"https:\/\/www.stemcell.com\/instantpurchase\/button\/placeOrder\/"}}}} } } </script> <div class="notifyPopup catalog-product-notify" style="display: none;"> <p class="form-declaration"> <div data-content-type="html" data-appearance="default" data-element="main" data-decoded="true">By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our <a href="/privacy-policy" target="_blank">privacy policy</a>. Please <a href="/contact-us" target="_blank">contact us</a> with any questions that you may have. You can unsubscribe or change your <a href="/customer/subscribe/" target="_blank">email preferences</a> at any time.</div> </p> </div> </div> <div class="wishlist-actions"> <script type="text/x-magento-init"> { "body": { "addToWishlist": {"productType":"configurable","giftcardInfo":"[id^=giftcard]"} } } </script> </div> </div> </div> <script type="text/x-magento-init"> { "#product_addtocart_form": { "Magento_Catalog/js/validate-product": {} } } </script> <div id="product-summaries"> <div class="summary open"> <div class="show-summary" ref="summary-advantages"> <h4>Product Advantages</h4><i class="fa fa-minus"></i><i class="fa fa-plus"></i> </div> <div class="summary-detail" id="summary-advantages" style="display:block;"> <ul><br /> <li>Enhanced buffering and stabilized FGF2 support cell quality while allowing for alternate feeding schedules</li><br /> <li>Supports superior culture morphology and cell growth characteristics</li><br /> <li>Enables heightened single-cell survival when used with CloneR™</li><br /> <li>Fully compatible with established genome editing and differentiation protocols</li><br /> </ul> </div> </div> <div class="summary close"> <div class="show-summary" ref="summary-components"> <h4>What's Included</h4><i class="fa fa-minus"></i><i class="fa fa-plus"></i> </div> <div class="summary-detail" id="summary-components" > <ul> <li>mTeSR™ Plus Kit, cGMP (Catalog #100-0276)</li> <ul> <li>mTeSR™ Plus Basal Medium, 400 mL</li> <li>mTeSR™ Plus 5X Supplement, 100 mL</li> </ul> </ul> <ul> <li>mTeSR™ Plus Kit (Catalog #(100-1130)</li> <ul> <li>mTeSR™ Plus Basal Medium, 800 mL</li> <li>mTeSR™ Plus 5x Supplement, 2 x 100 mL</li> </ul> </ul> </div> </div> </div> </div> </form> </div> <script> require([ 'jquery', 'priceBox' ], function($){ const dataPriceBoxSelector = '[data-role=priceBox]', dataProductIdSelector = '[data-product-id=32119]'; var priceBoxes = $(dataPriceBoxSelector + dataProductIdSelector); priceBoxes = priceBoxes.filter(function(index, elem){ return !$(elem).find('.price-from').length; }); priceBoxes.priceBox({'priceConfig': {"productId":32119,"priceFormat":{"pattern":"%s CAD","precision":2,"requiredPrecision":2,"decimalSymbol":".","groupSymbol":",","groupLength":3,"integerRequired":false},"prices":{"baseOldPrice":{"amount":331,"adjustments":[]},"oldPrice":{"amount":331,"adjustments":[]},"basePrice":{"amount":331,"adjustments":[]},"finalPrice":{"amount":331,"adjustments":[]}},"idSuffix":"_clone","tierPrices":[],"calculationAlgorithm":"TOTAL_BASE_CALCULATION"}}); }); </script> <script type="text/x-magento-init"> { "*": { "Stemcell_SearchSpring/js/intelligentSuggestPlp": { "search_spring_site_id": "815jys", "sku_for_search_spring": "100-0276_C" } } } </script> <div id="request_price_form" class="hidden"> <div class="request-pricing-wrapper"> <div class="description-container"> <div class="description-wrapper"> <h1>Request Pricing</h1> <p> Thank you for your interest in <strong>this product</strong>. Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n): </p> <p> <i class="fa fa-check-circle"></i>Estimated delivery time for your area </p> <p> <i class="fa fa-check-circle"></i>Product sample or exclusive offer </p> <p> <i class="fa fa-check-circle"></i>In-lab demonstration </p> <div class="form-declaration"> <div data-content-type="html" data-appearance="default" data-element="main" data-decoded="true">By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our <a href="/privacy-policy" target="_blank">privacy policy</a>. Please <a href="/contact-us" target="_blank">contact us</a> with any questions that you may have. You can unsubscribe or change your <a href="/customer/subscribe/" target="_blank">email preferences</a> at any time.</div> </div> </div> </div> <div class="form-container"> <form id="rp_form" class="sc-form popup" method="post" data-mage-init='{"mage/validation/validation":{}}' action="/products/distributor/requestPricing"> <div class="form-list-wrapper"> <div class="message hidden"></div> <ul class="form-list"> <li class="fields row"> <div class="field odd"> <label>First Name</label> <div class="input-box"> <input type="text" id="first_name" name="first_name" value="" title="First Name" maxlength="255" class="input-text required" /> </div> </div> <div class="field even"> <label>Last Name</label> <div class="input-box"> <input type="text" id="last_name" name="last_name" value="" title="Last Name" maxlength="255" class="input-text required" /> </div> </div> </li> <li class="fields row"> <div class="field odd"> <label>Work Email Address</label> <div class="input-box"> <input type="text" 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<select name="country" id="country" class="odd"> <option value=""></option> <option value="AF" > Afghanistan </option> <option value="AL" > Albania </option> <option value="DZ" > Algeria </option> <option value="AS" > American Samoa </option> <option value="AD" > Andorra </option> <option value="AO" > Angola </option> <option value="AI" > Anguilla </option> <option value="AQ" > Antarctica </option> <option value="AG" > Antigua and Barbuda </option> <option value="AR" > Argentina </option> <option value="AM" > Armenia </option> <option value="AW" > Aruba </option> <option value="AU" > Australia </option> <option value="AT" > Austria </option> <option value="AZ" > Azerbaijan </option> <option value="BS" > Bahamas </option> <option value="BH" > Bahrain </option> <option value="BD" > Bangladesh </option> <option value="BB" > Barbados </option> <option value="BY" > Belarus </option> <option value="BE" > Belgium </option> <option value="BZ" > Belize </option> <option value="BJ" > Benin </option> <option value="BM" > Bermuda </option> <option value="BT" > Bhutan </option> <option value="BO" > Bolivia </option> <option value="BA" > Bosnia and Herzegowina </option> <option value="BW" > Botswana </option> <option value="BV" > Bouvet Island </option> <option value="BR" > Brazil </option> <option value="IO" > British Indian Ocean Territory </option> <option value="BN" > Brunei Darussalam </option> <option value="BG" > Bulgaria </option> <option value="BF" > Burkina Faso </option> <option value="BI" > Burundi </option> <option value="KH" > Cambodia </option> <option value="CM" > Cameroon </option> <option value="CA" > Canada </option> <option value="CV" > Cape Verde </option> <option value="KY" > Cayman Islands </option> <option value="CF" > Central African Republic </option> <option value="TD" > Chad </option> <option value="CL" > Chile </option> <option value="CN" > China </option> <option value="CX" > Christmas Island </option> <option value="CC" > Cocos (Keeling) Islands </option> <option value="CO" > Colombia </option> <option value="KM" > Comoros </option> <option value="CG" > Congo </option> <option value="CK" > Cook Islands </option> <option value="CR" > Costa Rica </option> <option value="CI" > Cote d'Ivoire </option> <option value="HR" > Croatia </option> <option value="CY" > Cyprus </option> <option value="CZ" > Czech Republic </option> <option value="DK" > Denmark </option> <option value="DJ" > Djibouti </option> <option value="DM" > Dominica </option> <option value="DO" > Dominican Republic </option> <option value="TP" > East Timor </option> <option value="EC" > Ecuador </option> <option value="EG" > Egypt </option> <option value="SV" > El Salvador </option> <option value="GQ" > Equatorial Guinea </option> <option value="ER" > Eritrea </option> <option value="EE" > Estonia </option> <option value="ET" > Ethiopia </option> <option value="FK" > Falkland Islands </option> <option value="FO" > Faroe Islands </option> <option value="FJ" > Fiji </option> <option value="FI" > Finland </option> <option value="FR" > France </option> <option value="FX" > France, Metropolitan </option> <option value="GF" > French Guiana </option> <option value="PF" > French Polynesia </option> <option value="TF" > French Southern Territories </option> <option value="GA" > Gabon </option> <option value="GM" > Gambia </option> <option value="GE" > Georgia </option> <option value="DE" > Germany </option> <option value="GH" > Ghana </option> <option value="GI" > Gibraltar </option> <option value="GR" > Greece </option> <option value="GL" > Greenland </option> <option value="GD" > Grenada </option> <option value="GP" > Guadeloupe </option> <option value="GU" > Guam </option> <option value="GT" > Guatemala </option> <option value="GN" > Guinea </option> <option value="GW" > Guinea-Bissau </option> <option value="GY" > Guyana </option> <option value="HT" > Haiti </option> <option value="HM" > Heard and Mc Donald Islands </option> <option value="HN" > Honduras </option> <option value="HK" selected="selected"> Hong Kong </option> <option value="HU" > Hungary </option> <option value="IS" > Iceland </option> <option value="IN" > India </option> <option value="ID" > Indonesia </option> <option value="IQ" > Iraq </option> <option value="IE" > Ireland </option> <option value="IL" > Israel </option> <option value="IT" > Italy </option> <option value="JM" > Jamaica </option> <option value="JP" > Japan </option> <option value="JO" > Jordan </option> <option value="KZ" > Kazakhstan </option> <option value="KE" > Kenya </option> <option value="KI" > Kiribati </option> <option value="KR" > Korea, Republic of </option> <option value="KW" > Kuwait </option> <option value="KG" > Kyrgyzstan </option> <option value="LA" > Laos </option> <option value="LV" > Latvia </option> <option value="LB" > Lebanon </option> <option value="LS" > Lesotho </option> <option value="LR" > Liberia </option> <option value="LY" > Libyan Arab Jamahiriya </option> <option value="LI" > Liechtenstein </option> <option value="LT" > Lithuania </option> <option value="LU" > Luxembourg </option> <option value="MO" > Macau </option> <option value="MK" > Macedonia </option> <option value="MG" > Madagascar </option> <option value="MW" > Malawi </option> <option value="MY" > Malaysia </option> <option value="MV" > Maldives </option> <option value="ML" > Mali </option> <option value="MT" > Malta </option> <option value="MH" > Marshall Islands </option> <option value="MQ" > Martinique </option> <option value="MR" > Mauritania </option> <option value="MU" > Mauritius </option> <option value="YT" > Mayotte </option> <option value="MX" > Mexico </option> <option value="FM" > Micronesia, Federated States of </option> <option value="MD" > Moldova, Republic of </option> <option value="MC" > Monaco </option> <option value="MN" > Mongolia </option> <option value="ME" > Montenegro </option> <option value="MS" > Montserrat </option> <option value="MA" > Morocco </option> <option value="MZ" > Mozambique </option> <option value="MM" > Myanmar (Burma) </option> <option value="NA" > Namibia </option> <option value="NR" > Nauru </option> <option value="NP" > Nepal </option> <option value="NL" > Netherlands </option> <option value="NC" > New Caledonia </option> <option value="NZ" > New Zealand </option> <option value="NI" > Nicaragua </option> <option value="NE" > Niger </option> <option value="NG" > Nigeria </option> <option value="NU" > Niue </option> <option value="NF" > Norfolk Island </option> <option value="MP" > Northern Mariana Islands </option> <option value="NO" > Norway </option> <option value="OM" > Oman </option> <option value="PK" > Pakistan </option> <option value="PW" > Palau </option> <option value="PA" > Panama </option> <option value="PG" > Papua New Guinea </option> <option value="PY" > Paraguay </option> <option value="PE" > Peru </option> <option value="PH" > Philippines </option> <option value="PN" > Pitcairn </option> 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<div class="scientist-quotes-section full-width"> <div class="fixed-width"> <div class="swiper scientist-quotes"> <div class="swiper-wrapper"> <div class="scientist-quote swiper-slide"> <div class="quote-info"> <h2>What Our Scientist Says</h2> <p>Innovation is a core value at STEMCELL. The evolution to mTeSR™ Plus reflects that and our desire to serve the next generation of pluripotent stem cell researchers.</p> </div> <div class="scientist-info"> <span class="name-title">Matthew Wong</span>Scientist </div> <div class="scientist-photo round-image"> <img src="https://www.stemcell.com/media/images/scientists/Matthew_Wong.jpg" alt="Matthew Wong, Scientist"> </div> </div> </div> <div class="swiper-pagination"></div> </div> </div> </div> <div class="product info detailed"> <div class="product data items"> <div class="titles-row anchor-menu swiper"> <div class="data-anchors swiper-wrapper"> <div class="data item title swiper-slide" data-role="collapsible" id="tab-label-description"> <a class="data switch" tabindex="-1" data-toggle="switch" href="#section-overview" id="tab-label-description-title"> Overview </a> </div> <div class="data item title swiper-slide" data-role="collapsible" id="tab-label-product.info.data_figures"> <a class="data switch" tabindex="-1" data-toggle="switch" href="#section-data-figures" id="tab-label-product.info.data_figures-title"> Data Figures </a> </div> <div class="data item title swiper-slide" data-role="collapsible" id="tab-label-product.info.protocols_and_documentation"> <a class="data switch" tabindex="-1" data-toggle="switch" href="#section-protocols-and-documentation" id="tab-label-product.info.protocols_and_documentation-title"> Protocols and Documentation </a> </div> <div class="data item title swiper-slide" data-role="collapsible" id="tab-label-product.info.applications"> <a class="data switch" tabindex="-1" data-toggle="switch" href="#section-applications" id="tab-label-product.info.applications-title"> Applications </a> </div> <div class="data item title swiper-slide" data-role="collapsible" id="tab-label-product.info.resources_and_publications"> <a class="data switch" tabindex="-1" data-toggle="switch" href="#section-resources-and-publications" id="tab-label-product.info.resources_and_publications-title"> Resources and Publications </a> </div> <div class="data item title swiper-slide" data-role="collapsible" id="tab-label-product.info.related_product"> <a class="data switch" tabindex="-1" data-toggle="switch" href="#section-related-products" id="tab-label-product.info.related_product-title"> Related Products </a> </div> </div> </div> <div class="data item content fixed-width" id="section-overview" data-role="content"> <h2>Overview</h2> <div class="row view-more-content" data-visible-height="250" style="max-height:274px; overflow:hidden;"> <div class="grid_9_lg grid_9_md grid_6_sm grid_12_xs"> <div class="description" itemprop="description"> Enjoy weekend-free schedules and enhanced growth characteristics while maintaining cell quality with this stabilized feeder-free maintenance medium for human pluripotent stem cells (hPSCs). <br /> <br /> Manufactured under relevant cGMPs, mTeSR™ Plus ensures the highest quality and consistency for fundamental research as well as for cell therapy and investigational new drug research applications. It is based on <a href="/products/mtesr1.html">mTeSR™1</a> (Catalog #85850), the most widely published feeder-free cell culture medium for hPSCs. With stabilized critical medium components, including FGF2, and enhanced pH buffering, you can use mTeSR™ Plus to maintain cell-quality attributes and increase cell expansion rates with either daily or restricted feeding. Each lot of mTeSR™ Plus 5X Supplement is used to prepare complete mTeSR™ Plus medium and then performance-tested in a culture assay using human pluripotent stem cells (hPSCs).<br /> <br /> mTeSR™ Plus is compatible with a variety of culture matrices, including Corning® Matrigel® hESC-Qualified Matrix and <a href="/products/vitronectin-xf.html">Vitronectin XF™</a> (Catalog #07180, developed and manufactured by Nucleus Biologics). <br /> <br /> For additional quality information, visit <a href="/compliance">www.stemcell.com/compliance</a>.<br /> <br /> To request a Letter of Authorization (LOA) for the FDA Master File for mTeSR™ Plus, <a href="/loa-request-dmf">click here</a>. </div> </div> <div class="grid_3_lg grid_3_md grid_6_sm grid_12_xs"> <div class="attribute-list"> <div class="attribute attribute-subtype"> <div class="header">Subtype</div> <div class="value">Specialized Media</div> </div> <div class="attribute attribute-cell_type"> <div class="header">Cell Type</div> <div class="value">Pluripotent Stem Cells</div> </div> <div class="attribute attribute-species"> <div class="header">Species</div> <div class="value">Human</div> </div> <div class="attribute attribute-application"> <div class="header">Application</div> <div class="value">Cell Culture, Expansion, Maintenance</div> </div> <div class="attribute attribute-brand"> <div class="header">Brand</div> <div class="value">TeSR</div> </div> <div class="attribute attribute-area_of_interest"> <div class="header">Area of Interest</div> <div class="value">Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology</div> </div> <div class="attribute attribute-formulation"> <div class="header">Formulation Category</div> <div class="value">Serum-Free</div> </div> </div> </div> <div class="clear"></div> <div class="view-more-wrapper hidden"> <a class="view-more-button"> View More </a> <a class="view-less-button"> View Less </a> </div> </div> </div> <div class="data item content full-width" id="section-data-figures" data-role="content"> <h2>Data Figures</h2> <div class="section data-figures"> <div class="data-figures-content view-more-content" data-visible-height="450" style="max-height:474px; overflow:hidden;"> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig1-updated.jpg" alt=""> </div> <p class="figure-description"><strong>Figure 1.</strong> mTeSR™ Plus Maintains Optimal pH Levels Throughout a Weekend-Free Protocol</p> <p class="figure-note">The pH of spent medium from hPSCs cultured in mTeSR™ Plus is higher than that of hPSCs cultured in mTeSR™1 and other flexible-feeding medium at similar cell densities. pH and cell numbers were measured after a 72-hour period without feeding. Range of cell numbers shown represent different densities that would be observed throughout a typical passage. This demonstrates that feeds can be skipped for two days at any time during routine maintenance using mTeSR™ Plus while maintaining a pH above 7.0. Note: Cultures were fed double the standard medium volume prior to the 72-hour period without feeds in all media and cell numbers are from one well of a 6-well plate.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig2-Updated.jpg" alt=""> </div> <p class="figure-description"><strong>Figure 2.</strong> mTeSR™ Plus Maintains Consistent Levels of FGF2 Throughout a Weekend-Free Protocol</p> <p class="figure-note">FGF2 levels remain high in mTeSR™ Plus when cultured at 37°C over a 72 hour time period. Measured by ELISA.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig3-updated.jpg" alt=""> </div> <p class="figure-description"><strong>Figure 3.</strong> mTeSR™ Plus Supports Higher Cell Numbers</p> <p class="figure-note">Growth curves were obtained for human ES (H9) cells cultured in mTeSR™1 or mTeSR™ Plus on Corning® Matrigel® matrix over 7 days with either daily feeds or restricted feeds. Growth curves were determined by seeding 20,000 cells per well of a 6-well plate as aggregates and counting the cell numbers each day in duplicate wells.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/fig4_data_web_ready.jpg" alt=""> </div> <p class="figure-description"><strong>Figure 4.</strong> Larger Colonies are Observed in mTeSR™ Plus Cultures</p> <p class="figure-note">The average colony size per passage (± SEM) was obtained for human ES (H1, H9) and iPS (STiPS-M001, WLS-1C) cells cultured in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds) on Corning® Matrigel® over 10 passages. Size was determined by measuring representative colony diameters at harvest. Note that this data is representative of cultures passaged at a 7-day passaging interval; smaller colony size should be expected if using shorter passaging intervals.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/fig5_data_web_ready_updated.jpg" alt=""> </div> <p class="figure-description"><strong>Figure 5.</strong> Normal human ES and iPS Cell Morphology is Observed in mTeSR™ Plus Cultures</p> <p class="figure-note">Images depict undifferentiated human hES (H1) and iPS (WLS-1C) cells cultured on Corning®️ Matrigel®️ matrix in mTeSR™1 with daily feeds or mTeSR™ Plus with restricted feeds. Cells retain the prominent nucleoli and high nuclear-to-cytoplasmic ratio characteristic of this cell type after 10 passages. Densely packed cells and multi-layering are prominent when cells are ready to be passaged.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig6-updated.jpg" alt=""> </div> <p class="figure-description"><strong>Figure 6.</strong> Cells Cultured in mTeSR™ Plus Medium with Restricted Feeding Express Undifferentiated Cell Markers</p> <p class="figure-note">Human ES (H1, H9) and iPS (WLS-1C, STiPS-M001) cells were characterized using flow cytometry for undifferentiated cell markers, (A) OCT3/4 and (B) TRA-1-60. Graphs show average expression (± SEM) results from analyses of duplicate wells every 5 passages for up to 10-15 passages in mTeSR™1 (daily feeds), or mTeSR™ Plus (restricted feeds).</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/fig7_data_web_ready_2.jpg" alt=""> </div> <p class="figure-description"><strong>Figure 7.</strong> Cells Maintained in mTeSR™ Plus with Restricted Feeding Have Comparable Differentiation Efficiencies to Cells Maintained in mTeSR™1</p> <p class="figure-note">Human ES (H1, H9) and iPS (WLS-1C, STiPS-M001) cells were maintained in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds). Cells were differentiated using directed differentiation protocols and subjected to flow cytometry analysis. Graphs show average expression (± SEM) results from the 4 cell lines. The markers used for flow cytometry for each germ layer are listed in the bar titles.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig8-Updated.jpg" alt=""> </div> <p class="figure-description"><strong>Figure 8.</strong> hPSCs Cultured in mTeSR™ Plus with Restricted Feeding Maintain a Normal Karyotype</p> <p class="figure-note">Karyograms of (A) human ES (H1) and (B) iPS (WLS-1C) cells cultured in mTeSR™ Plus for 30 passages shows a normal karyotype is retained.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig9-Updated.jpg" alt=""> </div> <p class="figure-description"><strong>Figure 9.</strong> High Cloning Efficiency of hPSCs in mTeSR™ Plus Supplemented with CloneR™</p> <p class="figure-note">hPSCs (H1, H9, WLS-1C, and STiPS-M001) plated in mTeSR™ Plus with CloneR™ demonstrate cloning efficiencies equal to or greater than hPSCs in mTeSR™1 with CloneR™. Cells were seeded at clonal density (25 cells/cm²) in mTeSR™1 or mTeSR™ Plus on CellAdhere™ Vitronectin™ XF™-coated plates. n ≧ 3 biological replicates.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig10-updated.jpg" alt="Cell morphology images of ES cells plated in mTeSR™1 and mTeSR™ Plus and supplemented with CloneR™ immediately following RNP electroporation."> </div> <p class="figure-description"><strong>Figure 10. </strong>Representative Cell Morphology 24 Hours After RNP Electroporation in mTeSR™1 and mTeSR™ Plus</p> <p class="figure-note">H1-eGFP ES cells were plated in (A) mTeSR™1 and (B) mTeSR™ Plus and supplemented with CloneR™ immediately following RNP electroporation. Images were taken 24 hours after electroporation.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig11-updated.jpg" alt="Cell images of human ES colonies plated in mTeSR™1 and mTeSR™ Plus and supplemented with CloneR™ on CellAdhere™ Vitronectin™ XF™-coated plates."> </div> <p class="figure-description"><strong>Figure 11. </strong>Clones Derived in mTeSR™ Plus are Larger and Ready to Be Picked at an Earlier Timepoint</p> <p class="figure-note">Representative images of human ES (H9) colonies taken 8 days following singlecell plating at clonal density (25 cells/cm²) in either (A) mTeSR™1 or (B) mTeSR™ Plus supplemented with CloneR™ on CellAdhere™ Vitronectin™ XF™-coated plates.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig12-updated.jpg" alt="Cell morphology images of neural progenitor cells maintained in mTeSR™1 or mTeSR™ Plus. Arrowheads point to clearly displayed neural rosettes after replating embryoid bodies."> </div> <p class="figure-description"><strong>Figure 12. </strong>Generation of Neural Progenitor Cells from hPSCs Maintained in mTeSR™ Plus</p> <p class="figure-note">Human ES (H9) and iPS (STiPS-M001) cells were maintained in (A) mTeSR™1 with daily feeds or (B) mTeSR™ Plus with restricted feeds and differentiated using an embryoid body (EB)-based protocol with STEMdiff™ SMADi Neural Induction Kit. Neural progenitor cells derived from hPSCs maintained in either mTeSR™1 or mTeSR™ Plus clearly display neural rosettes (arrowheads) after replating EBs.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig13-updated.jpg" alt="Immunocytochemistry image of a cerebral organoid cultured in mTeSR™ Plus and directed to cerebral organoids using the STEMdiff™ Cerebral Organoid Kit."> </div> <p class="figure-description"><strong>Figure 13. </strong>Generation of Cerebral Organoids from hPSCs Maintained in mTeSR™ Plus</p> <p class="figure-note">Human ES (H9) cells were cultured with mTeSR™ Plus and directed to cerebral organoids using the STEMdiff™ Cerebral Organoid Kit. Image shows apical progenitor marker SOX2 (purple) and neuronal marker TBR1 (green).</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig14-updated.jpg" alt="Density plots showing CD34+ and CD45+ expression and percentage of cells co-expressing CD34+ and CD45+ and graphs showing total number of viable cells harvested."> </div> <p class="figure-description"><strong>Figure 14. </strong>Generation of Hematopoietic Progenitor Cells from hPSCs Maintained in mTeSR™ Plus</p> <p class="figure-note">Human ES (H1, H9) and iPS (STiPS-M001, WLS-1C) cell lines maintained in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds) were differentiated to hematopoietic progenitor cells using the STEMdiff™ Hematopoietic Kit. At the end of the differentiation period, cells were harvested from the supernatant and analyzed by flow cytometry for co-expression of CD34+ and CD45+ . (A) Representative density plots showing CD34+ and CD45+ expression, (B) percentage of cells co-expressing CD34+ and CD45+ , and (C) total number of viable cells harvested are shown. Data are expressed as the mean (± SEM); n=4.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig15-updated.jpg" alt="Microelectrode array and flow cytometry of human ES and iPS cells maintained in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds) and differentiated to cardiomyocytes using the STEMdiff™ Cardiomyocyte Differentiation Kit."> </div> <p class="figure-description"><strong>Figure 15. </strong>Generation of Cardiomyocytes from hPSCs Maintained in mTeSR™ Plus</p> <p class="figure-note">Human ES (H9) and iPS (WLS-1C) cells were maintained in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds) and differentiated to cardiomyocytes using the STEMdiff™ Cardiomyocyte Differentiation Kit. At the end of the differentiation period, cells were harvested and analyzed by microelectrode array (MEA) and flow cytometry. (A) Representative MEA voltage recordings of cardiomyocytes (day 20) demonstrate a characteristic electrical profile and stable beat rate. (B) Percentages of cells expressing cTNT and (C) total number of viable cells harvested are shown. Data are expressed as the mean (± SEM); n=2.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig16-updated.jpg" alt="Immunocytochemistry image of an intestinal organoid cultured in mTeSR™ Plus and directed to intestinal organoids using the STEMdiff™ Intestinal Organoid Kit."> </div> <p class="figure-description"><strong>Figure 16. </strong>Generation of Intestinal Organoids from hPSCs Maintained in mTeSR™ Plus</p> <p class="figure-note">Human ES (H9) cells were cultured with mTeSR™ Plus and directed to intestinal organoids using the STEMdiff™ Intestinal Organoid Kit. Image shows markers of the intestinal epithelium EpCAM (green) and CDX2 (red), and intestinal mesenchyme marker vimentin (white). Nuclei are counterstained with DAPI (blue).</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig17-updated.jpg" alt="Density plots and quantitative analysis showing CXCR4 and SOX17 expression in cells cultured in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds), following 5 days of differentiation using the STEMdiff™ Definitive Endoderm Kit."> </div> <p class="figure-description"><strong>Figure 17. </strong>Generation of Definitive Endoderm from hPSCs Maintained in mTeSR™ Plus</p> <p class="figure-note">(A) Representative density plots showing CXCR4 and SOX17 expression in cells cultured in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds), following 5 days of differentiation using the STEMdiff™ Definitive Endoderm Kit. (B) Quantitative analysis of definitive endoderm formation in multiple hPSC lines (H9, STiPS-M001, WLS-1C) maintained with mTeSR™1 or mTeSR™ Plus as measured by co-expression of CXCR4 and SOX17. Data are expressed as the mean percentage of cells (± SEM) expressing both markers; n=3.</p> </div> </div> <div class="section"> <div class="figure"> <div class="figure-image retina"> <img loading="lazy" src="/media/images/data-figures/05825/05825-Fig18-updated.jpg" alt="Density plots and quantitative analysis showing PDX-1 and NKX6.1 expression in cells cultured in mTeSR™1 or mTeSR™ Plus, following 5 days of differentiation using the STEMdiff™ Pancreatic Progenitor Kit."> </div> <p class="figure-description"><strong>Figure 18. </strong>Generation of Pancreatic Progenitors from hPSCs Maintained in mTeSR™ Plus</p> <p class="figure-note">(A) Representative density plots showing PDX-1 and NKX6.1 expression in cells cultured in mTeSR™1 (daily feeds) or mTeSR™ Plus (restricted feeds), following differentiation using the STEMdiff™ Pancreatic Progenitor Kit. (B) Quantitative analysis of pancreatic progenitor formation in multiple hPS (H9, STiPS-M001, WLS-1C) cell lines maintained with mTeSR™1 or mTeSR™ Plus as measured by co-expression of PDX-1 and NKX6.1. Data are expressed as the mean percentage of cells (± SEM) expressing both markers; n=3.</p> </div> </div> <div class="view-more-wrapper hidden"> <a class="view-more-button"> View More </a> <a class="view-less-button"> View Less </a> </div> </div> </div> </div> <div class="data item content fixed-width" id="section-protocols-and-documentation" data-role="content"> <h2>Protocols and Documentation</h2> <div class="product-resources"> <p> Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below. </p> <div class="table scientific-resources-table view-more-content" data-visible-height="500" style="max-height:524px; overflow:hidden;"> <div class="thead"> <div class="tr"> <div class="th document-type">Document Type</div> <div class="th product-name">Product Name</div> <div class="th catalog-number">Catalog #</div> <div class="th lot-number">Lot #</div> <div class="th language">Language</div> <div class="clear"></div> </div> </div> <div class="tbody"> <div class="tr"> <div class="td document-type"> <div class="th">Document Type</div> <a href="https://cdn.stemcell.com/media/files/pis/10000007758-PIS_03.pdf" target="_blank"> <i class="fa fa-file-pdf-o"></i> <span>Product Information Sheet </span> </a> </div> <div class="td product-name"> <div class="th">Product Name</div> <a href="https://cdn.stemcell.com/media/files/pis/10000007758-PIS_03.pdf" target="_blank"> mTeSR™ Plus </a> </div> <div class="td catalog-number"> <div class="th">Catalog #</div> 100-0276, 100-1130 </div> <div class="td lot-number"> <div class="th">Lot #</div> All </div> <div class="td language"> <div class="th">Language</div> English </div> <div class="clear"></div> </div> <div class="tr"> <div class="td document-type"> <div class="th">Document Type</div> <a href="https://cdn.stemcell.com/media/files/manual/10000007757-Maintenance_of_Human_Pluripotent_Stem_Cells_in_mTeSR_Plus.pdf" target="_blank"> <i class="fa fa-file-pdf-o"></i> <span>Technical Manual </span> </a> </div> <div class="td product-name"> <div class="th">Product Name</div> <a href="https://cdn.stemcell.com/media/files/manual/10000007757-Maintenance_of_Human_Pluripotent_Stem_Cells_in_mTeSR_Plus.pdf" target="_blank"> mTeSR™ Plus </a> </div> <div class="td catalog-number"> <div class="th">Catalog #</div> 100-0276 </div> <div class="td lot-number"> <div class="th">Lot #</div> All </div> <div class="td language"> <div class="th">Language</div> English </div> <div class="clear"></div> </div> <div class="tr"> <div class="td document-type"> <div class="th">Document Type</div> <a href="https://cdn.stemcell.com/media/files/pis/10000007945-SDS_1_1.pdf" target="_blank"> <i class="fa fa-file-pdf-o"></i> <span>Safety Data Sheet 1</span> </a> </div> <div class="td product-name"> <div class="th">Product Name</div> <a href="https://cdn.stemcell.com/media/files/pis/10000007945-SDS_1_1.pdf" target="_blank"> mTeSR™ Plus </a> </div> <div class="td catalog-number"> <div class="th">Catalog #</div> 100-0276, 100-1130 </div> <div class="td lot-number"> <div class="th">Lot #</div> All </div> <div class="td language"> <div class="th">Language</div> English </div> <div class="clear"></div> </div> <div class="tr"> <div class="td document-type"> <div class="th">Document Type</div> <a href="https://cdn.stemcell.com/media/files/pis/10000007909-SDS_1_1.pdf" target="_blank"> <i class="fa fa-file-pdf-o"></i> <span>Safety Data Sheet 2</span> </a> </div> <div class="td product-name"> <div class="th">Product Name</div> <a href="https://cdn.stemcell.com/media/files/pis/10000007909-SDS_1_1.pdf" target="_blank"> mTeSR™ Plus </a> </div> <div class="td catalog-number"> <div class="th">Catalog #</div> 100-0276, 100-1130 </div> <div class="td lot-number"> <div class="th">Lot #</div> All </div> <div class="td language"> <div class="th">Language</div> English </div> <div class="clear"></div> </div> <div class="tr"> <div class="td document-type"> <div class="th">Document Type</div> <a href="https://cdn.stemcell.com/media/files/pis/10000017374_SDS_1_0.pdf" target="_blank"> <i class="fa fa-file-pdf-o"></i> <span>Safety Data Sheet 1</span> </a> </div> <div class="td product-name"> <div class="th">Product Name</div> <a href="https://cdn.stemcell.com/media/files/pis/10000017374_SDS_1_0.pdf" target="_blank"> mTeSR™ Plus </a> </div> <div class="td catalog-number"> <div class="th">Catalog #</div> 100-1130 </div> <div class="td lot-number"> <div class="th">Lot #</div> All </div> <div class="td language"> <div class="th">Language</div> English </div> <div class="clear"></div> </div> </div> <div class="clear"></div> <div class="view-more-wrapper hidden"> <a class="view-more-button"> View More </a> <a class="view-less-button"> View Less </a> </div> </div> </div> <div class="coa-request-section"> <div class="product-additional-info note-box"> <i class="fa fa-info-circle"></i> <span> The Certificate of Analysis for this product has been updated for newly released materials. To access respective CoAs please <a class="coa-link" href="/coa">use this tool</a>. </span> </div> <style> .page-layout-1column .info.detailed .product.data.items .content .coa-request-section a.coa-link { border: none; border-radius: 0; color: #ef8200; padding: 0px; font-size: initial; display: initial; margin-top: 0px } @media (max-width: 767px) { .page-layout-1column .info.detailed .product.data.items .content .coa-request-section a.coa-link { font-size: 14px; line-height: 20px; } } </style> </div> </div> <div class="data item content fixed-width" id="section-applications" data-role="content"> <h2>Applications</h2> <div id="product-use" class="section"> <p class="description"> This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area. </p> <div class="table tablet view-more-content" data-visible-height="350" style="max-height:374px; overflow:hidden;"> <div class="thead"> <div class="tr"> <div class="th workflow-name"> <span>Research Area</span> </div> <div class="th steps"> <span>Workflow Stages</span> </div> <div class="clear"></div> </div> </div> <div class="tbody"> <div class="tr swiper workflow-steps" id="workflow-step-1"> <div class="td workflow-name"> <span class="th mobile">Workflow Stages for</span> <a href="https://www.stemcell.com/product-portfolios/human-pluripotent-stem-cell-research.html"> Human Pluripotent Stem Cell Research </a> </div> <div class="td steps swiper-wrapper"> <div class="step swiper-slide"> <a href="https://www.stemcell.com/product-portfolios/human-pluripotent-stem-cell-research/reprogramming.html"> <span>Reprogramming</span> </a> </div> <div class="step active swiper-slide"> <a href="https://www.stemcell.com/product-portfolios/human-pluripotent-stem-cell-research/maintenance.html"> <span>Maintenance</span> </a> </div> <div class="step swiper-slide"> <a href="https://www.stemcell.com/product-portfolios/human-pluripotent-stem-cell-research/differentiation.html"> <span>Differentiation</span> </a> </div> </div> <div class="swiper-scrollbar"></div> </div> <div class="tr swiper workflow-steps" id="workflow-step-2"> <div class="td workflow-name"> <span class="th mobile">Workflow Stages for</span> <a href="https://www.stemcell.com/product-portfolios/psc-quality-control.html"> PSC Quality Control </a> </div> <div class="td steps swiper-wrapper"> <div class="step active swiper-slide"> <a href="https://www.stemcell.com/product-portfolios/psc-quality-control/view-all.html"> <span>View All</span> </a> </div> </div> <div class="swiper-scrollbar"></div> </div> <div class="tr swiper workflow-steps" id="workflow-step-3"> <div class="td workflow-name"> <span class="th mobile">Workflow Stages for</span> <a href="https://www.stemcell.com/product-portfolios/genome-editing.html"> Genome Editing </a> </div> <div class="td steps swiper-wrapper"> <div class="step active swiper-slide"> <a href="https://www.stemcell.com/product-portfolios/genome-editing/view-all.html"> <span>View All</span> </a> </div> </div> <div class="swiper-scrollbar"></div> </div> </div> <div class="clear"></div> <div class="view-more-wrapper hidden"> <a class="view-more-button"> View More </a> <a class="view-less-button"> View Less </a> </div> </div> </div> </div> <div class="data item content full-width" id="section-resources-and-publications" data-role="content"> <h2>Resources and Publications</h2> <div class="educational-materials educational-materials-section swiper"> <h2 class="mobile-popup-trigger"> Educational Materials <span>(53)</span> </h2> <div class="materials mobile-popup swiper-wrapper"> <div class="tile swiper-slide"> <div class="material sync-height"> <div class="material-image"> <a href="https://cdn.stemcell.com/media/files/brochure/BR27158-mTeSR_Plus.pdf" target="_blank"> <div class="material-image-container"> <picture> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br27158-mtesr-plus.jpg?width=175&height=100&fit=crop" media="(min-width: 768px) and (max-width: 1007px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br27158-mtesr-plus.jpg?width=227&height=130&fit=crop" media="(min-width: 481px) and (max-width: 767px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br27158-mtesr-plus.jpg?width=204&height=115&fit=crop" media="(max-width: 480px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br27158-mtesr-plus.jpg?width=303&height=170&fit=crop"> <img loading="lazy" src="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br27158-mtesr-plus.jpg?width=303&height=170&fit=crop" width="303" height="170" alt="mTeSR™ Plus Product Overview Booklet" /> </picture> </div> </a> </div> <div class="type-name"> Brochure </div> <div class="material-name" title="mTeSR™ Plus Product Overview Booklet"> <a href="https://cdn.stemcell.com/media/files/brochure/BR27158-mTeSR_Plus.pdf" target="_blank"> mTeSR™ Plus Product Overview Booklet </a> </div> </div> </div> <div class="tile swiper-slide"> <div class="material sync-height"> <div class="material-image"> <a href="https://cdn.stemcell.com/media/files/brochure/BR29063-Products_for_Human_Pluripotent_Stem_Cells.pdf" target="_blank"> <div class="material-image-container"> <picture> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br174-psc_maintenance_brochure-thumbnail-500x280.jpg?width=175&height=100&fit=crop" media="(min-width: 768px) and (max-width: 1007px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br174-psc_maintenance_brochure-thumbnail-500x280.jpg?width=227&height=130&fit=crop" media="(min-width: 481px) and (max-width: 767px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br174-psc_maintenance_brochure-thumbnail-500x280.jpg?width=204&height=115&fit=crop" media="(max-width: 480px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br174-psc_maintenance_brochure-thumbnail-500x280.jpg?width=303&height=170&fit=crop"> <img loading="lazy" src="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br174-psc_maintenance_brochure-thumbnail-500x280.jpg?width=303&height=170&fit=crop" width="303" height="170" alt="Products for Human Pluripotent Stem Cells" /> </picture> </div> </a> </div> <div class="type-name"> Brochure </div> <div class="material-name" title="Products for Human Pluripotent Stem Cells"> <a href="https://cdn.stemcell.com/media/files/brochure/BR29063-Products_for_Human_Pluripotent_Stem_Cells.pdf" target="_blank"> Products for Human Pluripotent Stem Cells </a> </div> </div> </div> <div class="tile swiper-slide"> <div class="material sync-height"> <div class="material-image"> <a href="https://cdn.stemcell.com/media/files/brochure/BR27214-hPSC_Derived_Cell_Therapy_Workflow.pdf" target="_blank"> <div class="material-image-container"> <picture> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/h/p/hpsc-derived-cell-therapy-workflow-27214.jpg?width=175&height=100&fit=crop" media="(min-width: 768px) and (max-width: 1007px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/h/p/hpsc-derived-cell-therapy-workflow-27214.jpg?width=227&height=130&fit=crop" media="(min-width: 481px) and (max-width: 767px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/h/p/hpsc-derived-cell-therapy-workflow-27214.jpg?width=204&height=115&fit=crop" media="(max-width: 480px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/h/p/hpsc-derived-cell-therapy-workflow-27214.jpg?width=303&height=170&fit=crop"> <img loading="lazy" src="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/h/p/hpsc-derived-cell-therapy-workflow-27214.jpg?width=303&height=170&fit=crop" width="303" height="170" alt="hPSC-Derived Cell Therapy Workflow" /> </picture> </div> </a> </div> <div class="type-name"> Brochure </div> <div class="material-name" title="hPSC-Derived Cell Therapy Workflow"> <a href="https://cdn.stemcell.com/media/files/brochure/BR27214-hPSC_Derived_Cell_Therapy_Workflow.pdf" target="_blank"> hPSC-Derived Cell Therapy Workflow </a> </div> </div> </div> <div class="tile swiper-slide"> <div class="material sync-height"> <div class="material-image"> <a href="https://cdn.stemcell.com/media/files/brochure/BRDX20460-mTeSR1_cGMP_Grade.pdf" target="_blank"> <div class="material-image-container"> <picture> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/brdx20460_1.jpg?width=175&height=100&fit=crop" media="(min-width: 768px) and (max-width: 1007px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/brdx20460_1.jpg?width=227&height=130&fit=crop" media="(min-width: 481px) and (max-width: 767px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/brdx20460_1.jpg?width=204&height=115&fit=crop" media="(max-width: 480px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/brdx20460_1.jpg?width=303&height=170&fit=crop"> <img loading="lazy" src="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/brdx20460_1.jpg?width=303&height=170&fit=crop" width="303" height="170" alt="cGMP mTeSR™1" /> </picture> </div> </a> </div> <div class="type-name"> Brochure </div> <div class="material-name" title="cGMP mTeSR™1"> <a href="https://cdn.stemcell.com/media/files/brochure/BRDX20460-mTeSR1_cGMP_Grade.pdf" target="_blank"> cGMP mTeSR™1 </a> </div> </div> </div> <div class="tile swiper-slide"> <div class="material sync-height"> <div class="material-image"> <a href="https://cdn.stemcell.com/media/files/brochure/BR27242-hPSC_Differentiation.pdf" target="_blank"> <div class="material-image-container"> <picture> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br170-hpsc_differentiation_brochure-27242-thumbnail.jpg?width=175&height=100&fit=crop" media="(min-width: 768px) and (max-width: 1007px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br170-hpsc_differentiation_brochure-27242-thumbnail.jpg?width=227&height=130&fit=crop" media="(min-width: 481px) and (max-width: 767px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br170-hpsc_differentiation_brochure-27242-thumbnail.jpg?width=204&height=115&fit=crop" media="(max-width: 480px)"> <source srcset="https://www.stemcell.com/media/catalog/product/cache/a3b2788186157220f33afb50b11e7d2a/b/r/br170-hpsc_differentiation_brochure-27242-thumbnail.jpg?width=303&height=170&fit=crop"> <img loading="lazy" 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<div class="reference-info show-abstract"> <span class="reference-name"> Rapid and efficient induction of functional astrocytes from human pluripotent stem cells. </span> <span class="reference-author"> I. Canals et al. </span> <span class="reference-data"> Nature methods 2018 SEP </span> </div> <div class="actions"> <div class="show-abstract"> <i class="fa fa-plus"></i> </div> </div> </div> <div class="abstract"> <h4>Abstract</h4> <div class="abstract-content"> The derivation of astrocytes from human pluripotent stem cells is currently slow and inefficient. We demonstrate that overexpression of the transcription factors SOX9 and NFIB in human pluripotent stem cells rapidly and efficiently yields homogeneous populations of induced astrocytes. In our study these cells exhibited molecular and functional properties resembling those of adult human astrocytes and were deemed suitable for disease modeling. 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