Mouse CCL22/MDC DuoSet ELISA DY439: R&D Systems

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class="ds_title">Mouse CCL22/MDC DuoSet ELISA </h1> <div class="dsSprite_rnd ds_brand_logo ds_rnd_logo"></div> </div> <div class="product_meta"> <span class="category_number">Catalog #: DY439</span> <br/> <span class="citations"> <a href="#product-citations" id="citation_count" class="jump_to" data-tab="3" data-tabname="product-citations"> 11 Citations </a> </span> <span class="reviews review_stars_5"> <a href="#product-reviews" id="product_reviews" class="jump_to" data-tab="6" data-tabname="product-reviews"> 1 Review </a> </span> <a href="/reviews/DY439" rel="nofollow"><span class="submit_review"><button class="btn btn-quaternary">Submit a Review</button></span></a> <span class="datasheet_link"> <a href="#product-datasheets">Datasheet / COA / SDS</a> </span> </div>  <div class="product_wrapper row"> <div class="product_ordering_wrapper col-xs-12 col-md-8 col-md-push-4"> <div class="product_ordering"> <form action="/products/mouse-ccl22-mdc-duoset-elisa_dy439" method="post" id="atc-datasheet-form" accept-charset="UTF-8"><div><div id="atc_datasheet_block">     <table class="add_to_cart"> <thead> <tr> <th class="ds_catalog">Catalog #</th> <th class="ds_availability_column mobile-hide">Availability</th> <th class="ds_size_price_column">Size / Price</th> <th class="ds_quantity_column">Qty</th> </tr> </thead> <tbody> <tr class="et_atc_product" data-sku="DY439"> <td class="dat_catalog_column">DY439</td> <td class="et_atc_results" colspan="2"></td> <td class="ds_quantity ds_quantity_column"><div class="form-item form-item-catnum-dy439 form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-dy439" name="catnum-DY439" value="" size="5" maxlength="3" /></div></td> </tr> </tbody> </table> <div class="additional_products_header">Ancillary Products Available</div> <div class="additional_product_wrapper"><div class="et_atc_product" data-sku="DY008B"><div class="additional_product_label"><a href="/products/duoset-elisa-ancillary-reagent-kit-2_dy008b">DY008B: DuoSet ELISA Ancillary Reagent Kit 2</a></div><div class="et_atc_results"></div><div class="form-item form-item-catnum-dy008b form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-dy008b" name="catnum-DY008B" value="" size="5" maxlength="3" /></div></div></div><div class="additional_product_wrapper"><div class="et_atc_product" data-sku="DY999B"><div class="additional_product_label"><a href="/products/substrate-reagent-pack_dy999b">DY999B: Substrate Reagent Pack</a></div><div class="et_atc_results"></div><div class="form-item form-item-catnum-dy999b form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-dy999b" name="catnum-DY999B" value="" size="5" maxlength="3" /></div></div></div><div class="additional_product_wrapper"><div class="et_atc_product" data-sku="DY999"><div class="additional_product_label"><a href="/products/substrate-reagent-pack_dy999">DY999: Substrate Reagent Pack</a></div><div class="et_atc_results"></div><div class="form-item form-item-catnum-dy999 form-type-textfield form-group"><input class="atc_quantity atc_hidden atc_qty form-control form-text" placeholder="0" style="display:none;" type="text" id="edit-catnum-dy999" name="catnum-DY999" value="" size="5" maxlength="3" /></div></div></div> <div class="ds_atc_pre_buttons"></div> <div class="ds_atc_buttons"> <a href="/quote/instruments/ella-automated-immunoassay-system" class="btn btn-secondary atc_request_quote_simple_plex_assay" style="display:none" rel="nofollow">Request a Quote</a> <a href="/services/bulk-quote-request?catnum=DY439&product=Mouse CCL22/MDC DuoSet ELISA" class="btn btn-secondary atc_hidden bulk_btn" style="display:none;" rel="nofollow" title="Competitive discounts on products purchased in bulk quantities">Bulk Orders</a> <button class="btn btn-primary atc_hidden atc_btn form-submit icon-before" style="display:none;" title="Enter quantities above and click to add to cart" type="submit" id="edit-add-to-cart" name="add_to_cart_btn" value="Add to Cart"><span class="icon glyphicon glyphicon-plus" aria-hidden="true"></span> Add to Cart</button> </div> <div class="ds_atc_post_buttons"></div> </div> <input type="hidden" name="form_build_id" value="form-hUuGaQBqSiQHSh20w6D2TKwESemk5lOoPWzYuhJPH4w" /> <input type="hidden" name="form_id" value="atc_datasheet_form" /> </div></form> </div> </div> <div class="product_image_wrapper col-xs-12 col-md-4 col-md-pull-8"> <div class="product_image"> <span class="product_image_label"> </span> <a class="ds-image overview-images" data-toggle="lightbox" data-gallery="overview-images" onClick="event.preventDefault();" data-title="Mouse CCL22 / MDC ELISA Standard Curve" data-footer="Mouse CCL22 / MDC ELISA Standard Curve" title="Cat. No. DY439 - Mouse CCL22/MDC DuoSet ELISA" href="https://resources.rndsystems.com/images/datasheets/elisa/DATA_CCL22_DY439_ELISA_2027.jpg"> <img fetchpriority="high" src="https://resources.rndsystems.com/images/datasheets/elisa/DATA_CCL22_DY439_ELISA_2027.jpg" class="img-fluid" alt="Mouse CCL22 / MDC ELISA Standard Curve" title="Mouse CCL22 / MDC ELISA Standard Curve"> </a> <div class="search_image_other_count"> <span class="search_more_images">1 Image</span> </div> </div> </div> <div id="extra_images overview-images" class="hidden"> </div> </div> <div class="product_info_wrapper"> </div> <div id="view-more-target" class="datasheet_section_element"> <a href="/target/ccl22-mdc?category=" class="btn btn-secondary">All CCL22/MDC ELISAs</a> </div> </div> <div class="tabbed_content" id="rnd_elisabased"> <div id="ds_tabs" class="tab_menu"> <div class="tab tab_1 tab_open" data-tab="1" data-tabname="product-details" id="dsTabs_product-details"> <span>Product Details</span> </div> <div class="tab tab_2" data-tab="2" data-tabname="assay-procedure" id="dsTabs_assay-procedure"> <span>Procedure</span> </div> <div class="tab tab_3" data-tab="3" data-tabname="product-citations" id="dsTabs_product-citations"> <span>Citations (11)</span> </div> <div class="tab tab_4" data-tab="4" data-tabname="faqs" id="dsTabs_faqs"> <span>FAQs</span> </div> <div class="tab tab_5" data-tab="5" data-tabname="supplemental-products" id="dsTabs_supplemental-products"> <span>Supplemental Products</span> </div> <div class="tab tab_6" data-tab="6" data-tabname="product-reviews" id="dsTabs_product-reviews"> <span> Reviews (1) </span> </div> </div> <div class="tab_content tab_1 tab_open" data-tab="1" data-tabname="product-details" id="product-details"> <div class="datasheet_section_element"> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_summary" id="product-anchor-ds_summary">Summary</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_product_features" id="product-anchor-ds_product_features">Product Features</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_kit_content" id="product-anchor-ds_kit_content">Kit Content</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_other_reagents" id="product-anchor-ds_other_reagents">Other Reagents Required</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_data_examples" id="product-anchor-ds_data_examples">Data Examples</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_datasheets" id="product-anchor-ds_datasheets">Product Datasheets</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_preparation_storage" id="product-anchor-ds_preparation_storage">Preparation and Storage</a> <a class="btn btn-quaternary scroll_to anchor-link" href="#ds_background" id="product-anchor-ds_background">Background</a> </div> <div id="ds_summary" class="datasheet_section"> <h2>Mouse CCL22/MDC DuoSet ELISA Summary</h2> <div class="ds_table summary_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Type</div> <div class="ds_table_cell summary_table_data">Solid Phase Sandwich ELISA</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Format</div> <div class="ds_table_cell summary_table_data">96-well strip plate</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Sample Volume Required</div> <div class="ds_table_cell summary_table_data">100 µL</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Assay Range</div> <div class="ds_table_cell summary_table_data">7.8 - 500 pg/mL</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Sufficient Materials</div> <div class="ds_table_cell summary_table_data">For fifteen 96-well plates*</div> </div> <div class="ds_table_row"> <div class="ds_table_cell summary_table_name">Specificity</div> <div class="ds_table_cell summary_table_data">Please see the <a href="//resources.rndsystems.com/pdfs/datasheets/dy439.pdf" class="ds_pdf">product datasheet</a></div> </div> </div> </div> <p class="subtext">* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided. </p> <div id="ds_product_details" class="datasheet_section"> <p>This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CCL22/MDC. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.</p> </div> </div> <div id="customer_also_purchased_block" class="loader" data-catalogId="DY439"></div> <div id="ds_product_features" class="datasheet_section"> <h2>Product Features</h2> <ul> <li>Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time</li> <li>Development protocols are provided to guide further assay optimization</li> <li>Assay can be customized to your specific needs</li> <li>Economical alternative to complete kits</li> </ul> </div> <div id="ds_kit_content" class="datasheet_section"> <h2>Kit Content</h2> <ul> <li>Capture Antibody</li> <li>Detection Antibody</li> <li>Recombinant Standard</li> <li>Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)</li> </ul> </div> <div id="ds_other_reagents" class="datasheet_section"> <h2>Other Reagents Required</h2> <div class="other_reagent" id="reagents"><p><strong>DuoSet Ancillary Reagent Kit 2 (5 plates): </strong>(Catalog # <a href="/search?keywords=DY008">DY008</a>) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.</p> <p><strong>The components listed above may be purchased separately:</strong></p> <p><strong>PBS:</strong> (Catalog # <a href="/search?keywords=DY006">DY006</a>), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na<sub>2</sub>HPO<sub>4</sub>, 1.5 mM KH<sub>2</sub>PO<sub>4</sub>, pH 7.2 - 7.4, 0.2 µm filtered<br /> <br /> <strong>Wash Buffer:</strong> (Catalog # <a href="/search?keywords=WA126">WA126</a>), or 0.05% Tween<sup>®</sup> 20 in PBS, pH 7.2-7.4</p> <p><strong>Reagent Diluent: </strong>(Catalog # <a href="/search?keywords=DY995">DY995</a>), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered<br /> <br /> <strong>Substrate Solution:</strong> 1:1 mixture of Color Reagent A (H<sub>2</sub>O<sub>2</sub>) and Color Reagent B (Tetramethylbenzidine) (Catalog # <a href="/search?keywords=DY999">DY999</a>)<br /> <br /> <strong>Stop Solution:</strong> 2 N H<sub>2</sub>SO<sub>4</sub> (Catalog # <a href="/search?keywords=DY994">DY994</a>)</p> <p><strong>Microplates</strong>: R&D Systems (Catalog # <a href="/search?keywords=DY990">DY990</a>)<br /> <br /> <strong>Plate Sealers:</strong> ELISA Plate Sealers (Catalog # <a href="/search?keywords=DY992">DY992</a>)</p></div> </div> <div id="ds_scientific_data" class="datasheet_section"> <div id="ds_data_examples"> <h2>Scientific Data</h2> <div id="data_examples"> <div class="row data_ex_row"> <a id="ds_image_0" class="pageAnchor"></a> <div class="data_ex_image_wrapper col-xs-12 col-sm-5 col-md-4"> <div class="data_ex_image"> <span class="data_ex_image_label"> N/A </span> <a class="ds-image overview-images data_ex_image_link" data-toggle="lightbox" data-gallery="overview-images-0" data-title="Mouse CCL22 / MDC ELISA Standard Curve" data-footer="Mouse CCL22 / MDC ELISA Standard Curve" title="Cat. No. - Mouse CCL22 / MDC ELISA Standard Curve" href="https://resources.rndsystems.com/images/datasheets/elisa/DATA_CCL22_DY439_ELISA_2027.jpg"> <img loading="lazy" src="https://resources.rndsystems.com/images/datasheets/elisa/DATA_CCL22_DY439_ELISA_2027.jpg" class="img-fluid" alt="Mouse CCL22 / MDC ELISA Standard Curve" title="Mouse CCL22 / MDC ELISA Standard Curve"> <span class="data_ex_image_larger">View Larger</span> </a> </div> </div> <div class="data_ex_description col-xs-12 col-sm-7 col-md-8"> <p> <span class="data_ex_description_title">Mouse CCL22 / MDC ELISA Standard Curve</span> </p> </div> </div> </div> </div> </div> <div class="datasheet_section" id="product-datasheets"> <h2 id="ds_datasheets">Product Datasheets</h2> <div class="product_datasheets"> <span class="sds-message hide"> <p class="sds-label">You must select a language.</p> <span class="close_sds_message">x</span> </span> <div class="ds_document"> <a id="cfpdf" href="//resources.rndsystems.com/pdfs/datasheets/dy439.pdf?v=20241126" class="datasheets-link " target="_blank" rel="noreferrer nofollow">Product Datasheet</a> </div> <div class="ds_document"> <div class="ds_pdfData"><a class="datasheets-link" href="https://www.rndsystems.com/cofas/mouse-ccl22-mdc-duoset-elisa_dy439">COA</a> </div> </div> <div class="ds_document sds_link"> <a class="sds_non_cf datasheets-link non_cf_download_link" target="_blank" rel="noreferrer" data-path="//aero.bio-techne.com/sds/pdf/dy439?v=20241126">SDS</a> </div> </div> </div> <div id="ds_preparation_storage" class="datasheet_section"> <h2>Preparation and Storage</h2> <div class="ds_table prep_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Shipping</div> <div class="ds_table_cell prep_table_data">The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. </div> </div> <div class="ds_table_row"> <div class="ds_table_cell prep_table_name">Stability & Storage</div> <div class="ds_table_cell prep_table_data">Store the unopened product at 2 - 8 °C. Do not use past expiration date. </div> </div> </div> </div> </div> <div id="ds_background" class="datasheet_section"> <h2>Background: CCL22/MDC</h2> <p>Macrophage-derived chemokine (MDC), also named stimulated T cell chemotactic protein (STCP-1) and ABCD-1, and now designated as CCL22, is a CC chemokine initially isolated from clones of monocyte-derived macrophages. At the amino acid sequence level, MDC shows less than 35% identity to other CC chemokine family members. Human MDC is expressed in dendritic cells, macrophages and activated monocytes. In addition, MDC expression is detected in thymus, lymph node and appendix tissues. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity.</p> <div class="ds_table background_table"> <div class="ds_table_body"> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Entrez Gene IDs:</div> <div class="ds_table_cell background_table_data">6367 (Human); 20299 (Mouse)</div> </div> <div class="ds_table_row"> <div class="ds_table_cell background_table_name">Alternate Names:</div> <div class="ds_table_cell background_table_data">A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1</div> </div> </div> </div> </div> </div> <div class="tab_content tab_2" data-tab="2" data-tabname="product-assay-procedure" id="product-assay-procedure"> <a id="assay-procedure"></a> <div id="boxes">  <h2>Assay Procedure</h2> <h3><strong>GENERAL ELISA PROTOCOL</strong></h3> <h4><strong><em>Plate Preparation</em></strong></h4> <ol> <li>Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.</li> <li>Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.</li> <li>Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.</li> <li>Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.</li> </ol> <h4><strong><em>Assay Procedure</em></strong></h4> <ol> <li>Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.</li> <li>Repeat the aspiration/wash as in step 2 of Plate Preparation.</li> <li>Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.</li> <li>Repeat the aspiration/wash as in step 2 of Plate Preparation.</li> <li>Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.</li> <li>Repeat the aspiration/wash as in step 2.</li> <li>Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.</li> <li>Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.</li> <li>Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.</li> </ol> </div> </div> <div class="tab_content tab_3" data-tab="3" data-tabname="product-citations" id="citations"> <a id="citations_section"></a> <h2><span class="citations">Citations for Mouse CCL22/MDC DuoSet ELISA</h2> <div id="citation"> <a id="dsTabCitations"></a> <p> R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. </p> <p> <strong><span id="maxcitations">11</span> Citations: Showing <span id="showfrom">1</span> - <span id="showto">10</span> </strong> <br/><strong>Filter your results:</strong> </p> <div class="row"> <div class="col-sm-12"> <div class="citations_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper filter_select"> <select id="selSpecies" title="Species"> <option value="All">All Species</option> <option class="citation_filterRadio" value="species" data-filter="Human"> Human </option> <option class="citation_filterRadio" value="species" data-filter="Mouse"> Mouse </option> </select> </div> <input type="hidden" id="selAntibody" value="All"> <div class="select_wrapper filter_select"> <select id="selSampleType" title="Sample Types"> <option value="All">All Sample Types</option> <option class="citation_filterRadio" value="sampletype" data-filter="Cell Culture Supernates"> Cell Culture Supernates </option> <option class="citation_filterRadio" value="sampletype" data-filter="Plasma"> Plasma </option> <option class="citation_filterRadio" value="sampletype" data-filter="Serum"> Serum </option> <option class="citation_filterRadio" value="sampletype" data-filter="Serum, Cell Culture Supernates, Peritoneal Lavage Fluid"> Serum, Cell Culture Supernates, Peritoneal Lavage Fluid </option> <option class="citation_filterRadio" value="sampletype" data-filter="Tissue Homogenates"> Tissue Homogenates </option> </select> </div> </div> </div> </div> <div class="row"> <ol id="citations" class="col-xs-12" data-expandall="false" data-max-citations="11" data-max-show="10"> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum, Cell Culture Supernates, Peritoneal Lavage Fluid"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/38367238"> The IRAK1/IRF5 axis initiates IL-12 response by dendritic cells and control of Toxoplasma gondii infection </a> </span><br/> Authors: Pereira, M;Ramalho, T;Andrade, WA;Durso, DF;Souza, MC;Fitzgerald, KA;Golenbock, DT;Silverman, N;Gazzinelli, RT;<br/> Cell reports<br/> Species: Mouse<br/> Sample Types: Serum, Cell Culture Supernates, Peritoneal Lavage Fluid<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Serum"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/36969873"> Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinoma </a> </span><br/> Authors: W Zhang, X Jiang, Y Zou, L Yuan, X Wang<br/> Frontiers in Pharmacology, 2023-03-08;14(0):1092767.<br/> Species: Mouse<br/> Sample Types: Serum<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Plasma"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/36287123"> Combinatorial microRNA Loading into Extracellular Vesicles for Increased Anti-Inflammatory Efficacy </a> </span><br/> Authors: AE Pottash, D Levy, A Jeyaram, L Kuo, SM Kronstadt, W Chao, SM Jay<br/> Non-coding RNA, 2022-10-21;8(5):.<br/> Species: Mouse<br/> Sample Types: Plasma<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/32574210"> Head-to-head comparisons of Toxoplasma gondii and its near relative Hammondia hammondi reveal dramatic differences in the host response and effectors with species-specific functions </a> </span><br/> Authors: ZS Wong, SL Sokol-Borr, P Olias, JP Dubey, JP Boyle<br/> PLoS Pathog., 2020-06-23;16(6):e1008528.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/26204512"> S100B Up-Regulates Macrophage Production of IL1beta and CCL22 and Influences Severity of Retinal Inflammation. </a> </span><br/> Authors: Niven J, Hoare J, McGowan D, Devarajan G, Itohara S, Gannage M, Teismann P, Crane I<br/> PLoS ONE, 2015-07-23;10(7):e0132688.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Tissue Homogenates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/26075911"> Dipeptidylpeptidase 4 inhibition enhances lymphocyte trafficking, improving both naturally occurring tumor immunity and immunotherapy. </a> </span><br/> Authors: Barreira da Silva R, Laird M, Yatim N, Fiette L, Ingersoll M, Albert M<br/> Nat Immunol, 2015-06-15;16(8):850-8.<br/> Species: Mouse<br/> Sample Types: Tissue Homogenates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/25786174"> Network integration of parallel metabolic and transcriptional data reveals metabolic modules that regulate macrophage polarization. </a> </span><br/> Authors: Jha A, Huang S, Sergushichev A, Lampropoulou V, Ivanova Y, Loginicheva E, Chmielewski K, Stewart K, Ashall J, Everts B, Pearce E, Driggers E, Artyomov M<br/> Immunity, 2015-03-17;42(3):419-30.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/22539295"> Influence of hypoxia-inducible factor 1-alpha on dendritic cell differentiation and migration. </a> </span><br/> Authors: Kohler T, Reizis B, Johnson RS, Weighardt H, Forster I<br/> Eur. J. Immunol., 2012-05-01;42(5):1226-36.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/20557428"> Inflammatory chemokine release of astrocytes in vitro is reduced by all-trans retinoic acid. </a> </span><br/> Authors: van Neerven S, Regen T, Wolf D, Nemes A, Johann S, Beyer C, Hanisch UK, Mey J<br/> J. Neurochem., 2010-06-16;114(5):1511-26.<br/> Species: Mouse<br/> Sample Types: Cell Culture Supernates<br/> </li> <li class="top-lined" data-species="Human" data-application="" data-sampletype="Cell Culture Supernates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/18056384"> Mycobacterium tuberculosis antigens specifically modulate CCR2 and MCP-1/CCL2 on lymphoid cells from human pulmonary hilar lymph nodes. </a> </span><br/> Authors: Arias MA, Jaramillo G, Lopez YP, Mejia N, Mejia C, Pantoja AE, Shattock RJ, Garcia LF, Griffin GE<br/> J. Immunol., 2007-12-15;179(12):8381-91.<br/> Species: Human<br/> Sample Types: Cell Culture Supernates<br/> </li> <div id="ds_extra_cits"> <li class="top-lined" data-species="Mouse" data-application="" data-sampletype="Tissue Homogenates"> <span class="ctitle"> <a target="_blank" rel="noreferrer nofollow" class="safe_link" href="//www.ncbi.nlm.nih.gov/pubmed/16239514"> Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis. </a> </span><br/> Authors: Carpenter KJ, Hogaboam CM<br/> Infect. Immun., 2005-11-01;73(11):7198-207.<br/> Species: Mouse<br/> Sample Types: Tissue Homogenates<br/> </li> </div> </ol> </div> <div class="clearfix"><button class="btn btn-quinary ds_extra_cits pull-right toggle_cits_down" id="toggle_cits"></button></div> </div> </div> <div class="tab_content tab_4" data-tab="4" data-tabname="product-faqs" id="product-faqs"> <h2>FAQs</h2> <div class="row"> <div class="col-xs-12"> <p>No product specific FAQs exist for this product, however you may</p> <a class="btn btn-quinary view_all_faqs_btn" href="/resources/faqs/elisas"> View all ELISA FAQs </a> </div> </div> </div> <div class="tab_content tab_5" data-tab="5" data-tabname="product-supplemental" id="supplemental-products"> <div id="supplemental-products-tab-content-placeholder" class="tab-content-placeholder" data-id="supplemental-products" data-catalog_number="dy439" data-language="en"> <div class="et_spinner_small"></div> Loading... </div> </div> <div class="tab_content tab_6" data-tab="6" data-tabname="product-reviews" id="reviews"> <h3>Reviews for Mouse CCL22/MDC DuoSet ELISA</h3> <div class="row"> <div class="col-xs-12"> <div class="datasheet_section"> <div class="row"> <div class="col-md-6"> <p> Average Rating: 5 <span class="review_stars_5"></span> (Based on 1 Review) </p> <div class="review_rating_progress"> <div class="row"> <div class="col-xs-3"> 5 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="100" aria-valuemin="0" aria-valuemax="100" style="width:100%"> </div> </div> </div> <div class="col-xs-3"> 100% </div> </div> <div class="row"> <div class="col-xs-3"> 4 Star </div> <div class="col-xs-6"> <div class="progress"> <div class="progress-bar progress-bar-warning" role="progressbar" aria-valuenow="0" aria-valuemin="0" aria-valuemax="100" style="width:0%"> </div> </div> </div> <div class="col-xs-3"> 0% </div> </div> <div class="row"> <div class="col-xs-3"> 3 Star </div> <div 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Amazon gift card.</strong></h5> <div class="review_reward_txt"> <p>$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image</p> <p>$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image</p> </div> <a class="btn btn-quinary" href="/reviews/DY439"> Submit a Review </a> </div> </div> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <div class="reviews_filters"> <p style="display:inline;"><b>Filter by:</b></p> <div class="select_wrapper"> <select class="review_filter_rating"> <option value="all">All Ratings</option> <option value="5">5</option> </select> </div> <div class="select_wrapper"> <select class="review_filter_application"> <option value="all">All Applications</option> </select> </div> </div> </div> </div> <br/> <div class="review_row" data-rating="5" data-species="" data-application=""> <div class="row"> <div class="col-xs-12 col-sm-10"> <div class="review_title review_stars_5">Mouse CCL22/MDC DuoSet ELISA</div> <div class="review_date">By <span class="review_reviewer">Anonymous</span> on 04/15/2019 </div> <div class="row"> <div class="review_specifics col-xs-12"> <span class="ds_review_data"> <strong>Sample Tested: </strong> peritoneal lavage fluid </span> </div> </div> <div class="review_how_used"><p>Mice (HIII and LIII strains) injected i.p. with 2,4,6,10 tetramethylpentadecane (pristane).</p></div> </div> <div class="col-xs-12 col-sm-2"> <a class="ds-image" data-toggle="lightbox" href="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_dy439_42491_0.jpg" data-title="Mouse CCL22/MDC DuoSet ELISA DY439" data-gallery="Review-images"> <img loading="lazy" class="img-fluid review_image" alt="Mouse CCL22/MDC DuoSet ELISA DY439" title="Mouse CCL22/MDC DuoSet ELISA DY439" src="https://www.rndsystems.com/sites/rndsystems.com/files/reviews/review_dy439_42491_0.jpg"/> </a> </div> </div> <hr/> </div> </div> </div> </div> <div id="sidebar" class="col-xs-12 col-sm-4 col-md-3 col-lg-3 col-xl-3"> <div 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Mouse CCL22/MDC DuoSet ELISA DY439: R&D Systems