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Search results for: bacteriological diagnostics

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331</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: bacteriological diagnostics</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">331</span> Testing Immunochemical Method for the Bacteriological Diagnosis of Bovine Tuberculosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Assiya%20Madenovna%20Borsynbayeva">Assiya Madenovna Borsynbayeva</a>, <a href="https://publications.waset.org/abstracts/search?q=Kairat%20Altynbekovich%20Turgenbayev"> Kairat Altynbekovich Turgenbayev</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikolay%20Petrovich%20Ivanov"> Nikolay Petrovich Ivanov</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this article presents the results of rapid diagnostics of tuberculosis in comparison with classical bacteriological method. The proposed method of rapid diagnosis of tuberculosis than bacteriological method allows shortening the time of diagnosis to 7 days, to visualize the growth of mycobacteria in the semi-liquid medium and differentiate the type of mycobacterium. Fast definition of Mycobacterium tuberculosis and its derivatives in the culture medium is a new and promising direction in the diagnosis of tuberculosis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=animal%20diagnosis%20of%20tuberculosis" title="animal diagnosis of tuberculosis">animal diagnosis of tuberculosis</a>, <a href="https://publications.waset.org/abstracts/search?q=bacteriological%20diagnostics" title=" bacteriological diagnostics"> bacteriological diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=antigen" title=" antigen"> antigen</a>, <a href="https://publications.waset.org/abstracts/search?q=specific%20antibodies" title=" specific antibodies"> specific antibodies</a>, <a href="https://publications.waset.org/abstracts/search?q=immunological%20reaction" title=" immunological reaction"> immunological reaction</a> </p> <a href="https://publications.waset.org/abstracts/46923/testing-immunochemical-method-for-the-bacteriological-diagnosis-of-bovine-tuberculosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46923.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">344</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">330</span> Free Residual Chlorine and Bacteriological Contamination in Addis Ababa Water Supply System, Ethiopia</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aklilu%20Zeleke">Aklilu Zeleke</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A cross-sectional study was conducted in order to understand the effect of wet and dry seasons on the free residual chlorine and bacteriological contamination of the Addis Ababa (Ethiopia) water supply system. Water samples were taken at 30 selected distribution points and analyzed for Free Residual Chlorine and bacteriological analysis total coliforms and fecal coliform). It was found that some of the bacteriological data and Free Residual Chlorine levels are below the recommended values and beyond the maximum tolerable limits recommended by World Health Organization and Ethiopian National Standards. Water quality during the dry season is better than that of the wet season. There is a strong relationship between Free Residual Chlorine levels in drinking water and its bacteriological quality. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=addis%20ababa" title="addis ababa">addis ababa</a>, <a href="https://publications.waset.org/abstracts/search?q=wet%20season" title=" wet season"> wet season</a>, <a href="https://publications.waset.org/abstracts/search?q=dry%20season" title=" dry season"> dry season</a>, <a href="https://publications.waset.org/abstracts/search?q=free%20residual%20chlorine" title=" free residual chlorine"> free residual chlorine</a> </p> <a href="https://publications.waset.org/abstracts/168079/free-residual-chlorine-and-bacteriological-contamination-in-addis-ababa-water-supply-system-ethiopia" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/168079.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">78</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">329</span> Physicochemical and Bacteriological Quality Characterization of Some Selected Wells in Ado-Ekiti, Nigeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Olu%20Ale">Olu Ale</a>, <a href="https://publications.waset.org/abstracts/search?q=Olugbenga%20Aribisala"> Olugbenga Aribisala</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanmi%20Awopetu"> Sanmi Awopetu </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Groundwater (Wells) is obtained from several well-defined and different water-bearing geological layers or strata. The physical, chemical and bacteriological quality of the water contributed from each of these water-bearing formations and resultant effects of indiscriminate wastes disposal will be dependent on the dissolution of material within the formation. Therefore, water withdrawn from any ground water source will be a composite of these individual aquifers. The water quality was determined by actual sampling and analysis of the completed wells. This study attempted to examine the physicochemical and bacteriological water quality of twenty five selected wells comprising twenty boreholes (deep wells) and five hand dug wells (shallow wells). The twenty five wells cut across the entire Ado Ekiti Metropolitan area. The water samples collected using standard method was promptly taken to water laboratory at the Federal Polytechnic Ado-Ekiti for analysis, physical, chemical and bacteriological tests were carried out. Quality characteristics tested were found to meet WHO’s standard and generally acceptable, making it potable for drinking in most situations, thus encouraging the use of groundwater. Possible improvement strategies to groundwater exploitation were highlighted while remedies to poor quality water were suggested. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacteriological" title="bacteriological">bacteriological</a>, <a href="https://publications.waset.org/abstracts/search?q=physicochemical" title=" physicochemical"> physicochemical</a>, <a href="https://publications.waset.org/abstracts/search?q=quality" title=" quality"> quality</a>, <a href="https://publications.waset.org/abstracts/search?q=wells" title=" wells"> wells</a>, <a href="https://publications.waset.org/abstracts/search?q=Ado%20Ekiti" title=" Ado Ekiti"> Ado Ekiti</a> </p> <a href="https://publications.waset.org/abstracts/35884/physicochemical-and-bacteriological-quality-characterization-of-some-selected-wells-in-ado-ekiti-nigeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35884.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">368</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">328</span> Electronic Nose Based on Metal Oxide Semiconductor Sensors as an Alternative Technique for the Spoilage Classification of Oat Milk</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=A.%20Deswal">A. Deswal</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20S.%20Deora"> N. S. Deora</a>, <a href="https://publications.waset.org/abstracts/search?q=H.%20N.%20Mishra"> H. N. Mishra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of the present study was to develop a rapid method for electronic nose for online quality control of oat milk. Analysis by electronic nose and bacteriological measurements were performed to analyse spoilage kinetics of oat milk samples stored at room temperature and refrigerated conditions for up to 15 days. Principal component analysis (PCA), discriminant factorial analysis (DFA) and soft independent modelling by class analogy (SIMCA) classification techniques were used to differentiate the samples of oat milk at different days. The total plate count (bacteriological method) was selected as the reference method to consistently train the electronic nose system. The e-nose was able to differentiate between the oat milk samples of varying microbial load. The results obtained by the bacteria total viable counts showed that the shelf-life of oat milk stored at room temperature and refrigerated conditions were 20 hours and 13 days, respectively. The models built classified oat milk samples based on the total microbial population into “unspoiled” and “spoiled”. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electronic-nose" title="electronic-nose">electronic-nose</a>, <a href="https://publications.waset.org/abstracts/search?q=bacteriological" title=" bacteriological"> bacteriological</a>, <a href="https://publications.waset.org/abstracts/search?q=shelf-life" title=" shelf-life"> shelf-life</a>, <a href="https://publications.waset.org/abstracts/search?q=classification" title=" classification"> classification</a> </p> <a href="https://publications.waset.org/abstracts/2540/electronic-nose-based-on-metal-oxide-semiconductor-sensors-as-an-alternative-technique-for-the-spoilage-classification-of-oat-milk" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/2540.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">258</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">327</span> Bacteriological Analysis of Logan&#039;s Branch Rowan County, Kentucky Utilizing Membrane Filtration Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Elizabeth%20G.%20Hereford">Elizabeth G. Hereford</a>, <a href="https://publications.waset.org/abstracts/search?q=Geoffrey%20W.%20Gearner"> Geoffrey W. Gearner</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Logan’s Branch, within the Triplett Creek Watershed of Rowan County, Kentucky, is a waterway located near important agricultural and residential areas. Part of Logan’s Branch flows over an exposed black shale formation with elevated radioactivity and heavy metals. Three sites were chosen in relation to the formation and sampled five times over a thirty-day period during the recreational season. A fourth site in North Fork in Rowan County, Kentucky was also sampled periodically as it too has contact with the shale formation. These sites were then sampled monthly. All samples are analyzed for concentrations of Escherichia coli, heterotrophic bacteria, and total coliform bacteria utilizing the membrane filtration method and various culture media. Current data suggests that the radioactivity of the shale formation influences the bacteriological growth present in the waterway; however, further data will be collected and compared with that of my colleagues to confirm this trend. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacteriological%20analysis" title="bacteriological analysis">bacteriological analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=Escherichia%20coli" title=" Escherichia coli"> Escherichia coli</a>, <a href="https://publications.waset.org/abstracts/search?q=heterotrophic%20bacteria" title=" heterotrophic bacteria"> heterotrophic bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=radioactive%20black%20shale%20formation" title=" radioactive black shale formation"> radioactive black shale formation</a>, <a href="https://publications.waset.org/abstracts/search?q=water%20quality" title=" water quality"> water quality</a> </p> <a href="https://publications.waset.org/abstracts/86253/bacteriological-analysis-of-logans-branch-rowan-county-kentucky-utilizing-membrane-filtration-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/86253.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">188</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">326</span> Bacteriological and Mineral Analyses of Leachate Samples from Erifun Dumpsite, Ado-Ekiti, Ekiti State, Nigeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Adebowale%20T.%20Odeyemi">Adebowale T. Odeyemi</a>, <a href="https://publications.waset.org/abstracts/search?q=Oluwafemi%20A.%20Ajenifuja"> Oluwafemi A. Ajenifuja</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The leachate samples collected from Erifun dumpsite along Federal Polythenic road, Ado-Ekiti, Ekiti State, were subjected to bacteriological and mineral analyses. The bacteriological estimation and isolation were done using serial dilution and pour plating techniques. Antibiotic susceptibility test was done using agar disc diffusion technique. Atomic Absorption Spectophotometry method was used to analyze the heavy metal contents in the leachate samples. The bacterial and coliform counts ranged from 4.2 × 105 CFU/ml to 2.97 × 106 CFU/ml and 5.0 × 104 CFU/ml to 2.45 x 106 CFU/ml, respectively. The isolated bacteria and percentage of occurrence include Bacillus cereus (22%), Enterobacter aerogenes (18%), Staphylococcus aureus (16%), Proteus vulgaris (14%), Escherichia coli (14%), Bacillus licheniformis (12%) and Klebsiella aerogenes (4%). The mineral value ranged as follow; iron (21.30mg/L - 25.60mg/L), zinc (1.80mg/L - 5.60mg/L), copper (1.00mg/L - 2.60mg/L), chromium (0.50mg/L - 1.30mg/L), candium (0.20mg/L - 1.30mg/L), nickel (0.20mg/L - 0.80mg/L), lead (0.05mg/L-0.30mg/L), cobalt (0.03mg/L - 0.30mg/L) and in all samples manganese was not detected. The entire organisms isolated exhibited a high level of resistance to most of the antibiotics used. There is an urgent need for awareness to be created about the present situation of the leachate in Erifun, on the need for treatment of the nearby stream and other water sources before they can be used for drinking and other domestic use. In conclusion, a good method of waste disposal is required in those communities to prevent leachate formation, percolation, and runoff into water bodies during the raining season. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=antibiotic%20susceptibility" title="antibiotic susceptibility">antibiotic susceptibility</a>, <a href="https://publications.waset.org/abstracts/search?q=dumpsite" title=" dumpsite"> dumpsite</a>, <a href="https://publications.waset.org/abstracts/search?q=bacteriological%20analysis" title=" bacteriological analysis"> bacteriological analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=heavy%20metal" title=" heavy metal"> heavy metal</a> </p> <a href="https://publications.waset.org/abstracts/110105/bacteriological-and-mineral-analyses-of-leachate-samples-from-erifun-dumpsite-ado-ekiti-ekiti-state-nigeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/110105.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">141</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">325</span> Diagnostics via Biophysical Resistotrons</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Matt%20Vellkorn">Matt Vellkorn</a>, <a href="https://publications.waset.org/abstracts/search?q=Mara%20Sarinski"> Mara Sarinski</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The field of advanced diagnostics is a very rapidly changing one. A new technology that has not been fully used yet are resistotrons. A resistotron is a physical device thatis used to detect the presence of low energy alpha particles. It has been used for many years in nuclear physics as an alpha particle detector. Since they are used in nuclear physics, they have to be accurate. They have to be able to differentiate between alpha particles and other types of radiation. The resistotrons are primarily used for safety. They are used in areas where people or animals can get exposed to radiation. A typical example is in the treatment of nuclear waste. As it is with any nuclear physics instrument, a resistotron has to be very accurate and reliable. In the past, the instrument was very expensive because they were made out of copper. Today, they are made out of brass. The main difference is that brass is much less expensive than copper. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biosensors" title="biosensors">biosensors</a>, <a href="https://publications.waset.org/abstracts/search?q=resistotrons" title=" resistotrons"> resistotrons</a>, <a href="https://publications.waset.org/abstracts/search?q=biophysics" title=" biophysics"> biophysics</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnostics" title=" diagnostics"> diagnostics</a> </p> <a href="https://publications.waset.org/abstracts/148720/diagnostics-via-biophysical-resistotrons" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/148720.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">122</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">324</span> Assessment of Groundwater Quality in Karakulam Grama Panchayath in Thiruvananthapuram, Kerala State, South India</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=D.%20S.%20Jaya">D. S. Jaya</a>, <a href="https://publications.waset.org/abstracts/search?q=G.%20P.%20Deepthi"> G. P. Deepthi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Groundwater is vital to the livelihoods and health of the majority of the people since it provides almost the entire water resource for domestic, agricultural and industrial uses. Groundwater quality comprises the physical, chemical, and bacteriological qualities. The present investigation was carried out to determine the physicochemical and bacteriological quality of the ground water sources in the residential areas of Karakulam Grama Panchayath in Thiruvananthapuram district, Kerala state in India. Karakulam is located in the eastern suburbs of Thiruvananthapuram city. The major drinking water source of the residents in the study area are wells. The present study aims to assess the portability and irrigational suitability of groundwater in the study area. The water samples were collected from randomly selected dug wells and bore wells in the study area during post monsoon and pre-monsoon seasons of the year 2014 after a preliminary field survey. The physical, chemical and bacteriological parameters of the water samples were analysed following standard procedures. The concentration of heavy metals (Cd, Pb, and Mn) in the acid digested water samples were determined by using an Atomic Absorption Spectrophotometer. The results showed that the pH of well water samples ranged from acidic to the alkaline level. In the majority of well water samples ( > 54%) the iron and magnesium content were found high in both the seasons studied, and the values were above the permissible limits of WHO drinking water quality standards. Bacteriological analyses showed that 63% of the wells were contaminated with total coliforms in both the seasons studied. Irrigational suitability of groundwater was assessed by determining the chemical indices like Sodium Percentage (%Na), Sodium Adsorption Ratio (SAR), Residual Sodium Carbonate (RSC), Permeability Index (PI), and the results indicate that the well water in the study area is good for irrigation purposes. Therefore, the study reveals the degradation of drinking water quality groundwater sources in Karakulam Grama Panchayath in Thiruvananthapuram District, Kerala in terms of its chemical and bacteriological characteristics and is not potable without proper treatment. In the study, more than 1/3rd of the wells tested were positive for total coliforms, and the bacterial contamination may pose threats to public health. The study recommends the need for periodic well water quality monitoring in the study area and to conduct awareness programs among the residents. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bacteriological" title="bacteriological">bacteriological</a>, <a href="https://publications.waset.org/abstracts/search?q=groundwater" title=" groundwater"> groundwater</a>, <a href="https://publications.waset.org/abstracts/search?q=irrigational%20suitability" title=" irrigational suitability"> irrigational suitability</a>, <a href="https://publications.waset.org/abstracts/search?q=physicochemical" title=" physicochemical"> physicochemical</a>, <a href="https://publications.waset.org/abstracts/search?q=portability" title=" portability"> portability</a> </p> <a href="https://publications.waset.org/abstracts/30584/assessment-of-groundwater-quality-in-karakulam-grama-panchayath-in-thiruvananthapuram-kerala-state-south-india" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30584.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">263</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">323</span> Comparative Study of the Quality of Treated Water and Sludge from Wastewater Treatment Plants in the Peri-Urban Area of Casablanca</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Meryem%20Zarri">Meryem Zarri</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohame%20Tahiri"> Mohame Tahiri</a>, <a href="https://publications.waset.org/abstracts/search?q=Fouad%20Amraoui"> Fouad Amraoui</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the context of water resources shortage that Morocco is experiencing in recent years, the mobilization of non-conventional resources becomes a necessity. The reuse of treated water and the bioconversion of biological sewage sludge into value-added products is considered an environmentally friendly and economical approach to the management of this significant resource which represent at least 80 % of consumed fresh wate In this work, we compare the quality of treated water and sewage sludge from wastewater treatment plants in the peri-urban Casablanca by analyzing different physicochemical and bacteriological parameters. The choice was made for three wastewater plants installed in different regions and monitored either by LYDEC and Commune of Had Soualem and use different technologies. Recycling of treated water in agriculture and watering of green spaces is dependent on the compliance of the parameters with international standards (WHO, FAO, …etc.) The preliminary tests of the samples taken during the second half of the year 2021 showed that the advanced technologies put in place at the level of the Mediouna and the airport zone stations (membrane reactor and activated sludge, respectively) give water to the output of the stations more respectful of the standards required in terms of physicochemical parameters (pH, Conductivity, Tubidity, COD, BOD5, TNK, and TPK) and bacteriological (fecal germs, Escherichia Coli, streptococci, Helminthes eggs). The parameters relating to the Had Soualem natural lagoon station are generally at the tolerance’s threshold. The results of analyzes relating to the residual sludge collected at the end of the cycle are, on the whole satisfactory despite a fluctuating variability of the bacteriological parameters. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=urban%20wastewater%20treatment%20plants" title="urban wastewater treatment plants">urban wastewater treatment plants</a>, <a href="https://publications.waset.org/abstracts/search?q=purified%20wastewater" title=" purified wastewater"> purified wastewater</a>, <a href="https://publications.waset.org/abstracts/search?q=sewage%20sludge" title=" sewage sludge"> sewage sludge</a>, <a href="https://publications.waset.org/abstracts/search?q=physicochemical%20parameters" title=" physicochemical parameters"> physicochemical parameters</a>, <a href="https://publications.waset.org/abstracts/search?q=bacteriological%20parameters" title=" bacteriological parameters"> bacteriological parameters</a>, <a href="https://publications.waset.org/abstracts/search?q=peri-urban%20area%20of%20%E2%80%8B%E2%80%8Bcasablanca" title=" peri-urban area of ​​casablanca"> peri-urban area of ​​casablanca</a>, <a href="https://publications.waset.org/abstracts/search?q=morocco" title=" morocco"> morocco</a> </p> <a href="https://publications.waset.org/abstracts/148253/comparative-study-of-the-quality-of-treated-water-and-sludge-from-wastewater-treatment-plants-in-the-peri-urban-area-of-casablanca" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/148253.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">153</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">322</span> Research Design for Developing and Validating Ice-Hockey Team Diagnostics Scale</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Gergely%20Geczi">Gergely Geczi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In the modern world, ice hockey (and, in a broader sense, team sports) is becoming an increasingly popular field of entertainment. Although the main element is most likely perceived as the show itself, winning is an inevitable part of the successful operation of any sports team. In this paper, the author creates a research design allowing him to develop and validate an ice-hockey team-focused diagnostics scale, which enables researchers and practitioners to identify the problems associated with underperforming teams. The construction of the scale starts with personal interviews with experts of the field, carefully chosen from the sector of Hungarian ice hockey. Based on the interviews, the author is shown to be in the position to create the categories and the relevant items for the scale. When constructed, the next step is the validation process on a Hungarian sample. Data for validation are acquired through reaching the licensed database of the Hungarian Ice-Hockey Federation involving Hungarian ice-hockey coaches and players. The Ice-Hockey Team Diagnostics Scale is to be created to orient practitioners in understanding both effective and underperforming teamwork. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diagnostics%20scale" title="diagnostics scale">diagnostics scale</a>, <a href="https://publications.waset.org/abstracts/search?q=effective%20versus%20underperforming%20team%20work" title=" effective versus underperforming team work"> effective versus underperforming team work</a>, <a href="https://publications.waset.org/abstracts/search?q=ice-hockey" title=" ice-hockey"> ice-hockey</a>, <a href="https://publications.waset.org/abstracts/search?q=research%20design" title=" research design"> research design</a> </p> <a href="https://publications.waset.org/abstracts/132475/research-design-for-developing-and-validating-ice-hockey-team-diagnostics-scale" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/132475.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">132</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">321</span> Photoplethysmography-Based Device Designing for Cardiovascular System Diagnostics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Botman">S. Botman</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Borchevkin"> D. Borchevkin</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20Petrov"> V. Petrov</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Bogdanov"> E. Bogdanov</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Patrushev"> M. Patrushev</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20Shusharina"> N. Shusharina</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In this paper, we report the development of the device for diagnostics of cardiovascular system state and associated automated workstation for large-scale medical measurement data collection and analysis. It was shown that optimal design for the monitoring device is wristband as it represents engineering trade-off between accuracy and usability. The monitoring device is based on the infrared reflective photoplethysmographic sensor, which allows collecting multiple physiological parameters, such as heart rate and pulsing wave characteristics. Developed device use BLE interface for medical and supplementary data transmission to the coupled mobile phone, which process it and send it to the doctor's automated workstation. Results of this experimental model approbation confirmed the applicability of the proposed approach. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cardiovascular%20diseases" title="cardiovascular diseases">cardiovascular diseases</a>, <a href="https://publications.waset.org/abstracts/search?q=health%20monitoring%20systems" title=" health monitoring systems"> health monitoring systems</a>, <a href="https://publications.waset.org/abstracts/search?q=photoplethysmography" title=" photoplethysmography"> photoplethysmography</a>, <a href="https://publications.waset.org/abstracts/search?q=pulse%20wave" title=" pulse wave"> pulse wave</a>, <a href="https://publications.waset.org/abstracts/search?q=remote%20diagnostics" title=" remote diagnostics"> remote diagnostics</a> </p> <a href="https://publications.waset.org/abstracts/29151/photoplethysmography-based-device-designing-for-cardiovascular-system-diagnostics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29151.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">492</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">320</span> Analysis of Transformer by Gas and Moisture Sensor during Laboratory Time Monitoring</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Miroslav%20Gutten">Miroslav Gutten</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20Korenciak"> Daniel Korenciak</a>, <a href="https://publications.waset.org/abstracts/search?q=Milan%20Simko"> Milan Simko</a>, <a href="https://publications.waset.org/abstracts/search?q=Milan%20Chupac"> Milan Chupac</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ensure the reliable and correct function of transformers is the main essence of on-line non-destructive diagnostic tool, which allows the accurately track of the status parameters. Devices for on-line diagnostics are very costly. However, there are devices, whose price is relatively low and when used correctly, they can be executed a complex diagnostics. One of these devices is sensor HYDRAN M2, which is used to detect the moisture and gas content in the insulation oil. Using the sensor HYDRAN M2 in combination with temperature, load measurement, and physicochemical analysis can be made the economically inexpensive diagnostic system, which use is not restricted to distribution transformers. This system was tested in educational laboratory environment at measured oil transformer 22/0.4 kV. From the conclusions referred in article is possible to determine, which kind of fault was occurred in the transformer and how was an impact on the temperature, evolution of gases and water content. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=transformer" title="transformer">transformer</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnostics" title=" diagnostics"> diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=gas%20and%20moisture%20sensor" title=" gas and moisture sensor"> gas and moisture sensor</a>, <a href="https://publications.waset.org/abstracts/search?q=monitoring" title=" monitoring"> monitoring</a> </p> <a href="https://publications.waset.org/abstracts/77770/analysis-of-transformer-by-gas-and-moisture-sensor-during-laboratory-time-monitoring" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/77770.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">385</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">319</span> Pantograph-Catenary Contact Force: Features Evaluation for Catenary Diagnostics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mehdi%20Brahimi">Mehdi Brahimi</a>, <a href="https://publications.waset.org/abstracts/search?q=Kamal%20Medjaher"> Kamal Medjaher</a>, <a href="https://publications.waset.org/abstracts/search?q=Noureddine%20Zerhouni"> Noureddine Zerhouni</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20Leouatni"> Mohammed Leouatni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The Prognostics and Health Management is a system engineering discipline which provides solutions and models to the implantation of a predictive maintenance. The approach is based on extracting useful information from monitoring data to assess the “health” state of an industrial equipment or an asset. In this paper, we examine multiple extracted features from Pantograph-Catenary contact force in order to select the most relevant ones to achieve a diagnostics function. The feature extraction methodology is based on simulation data generated thanks to a Pantograph-Catenary simulation software called INPAC and measurement data. The feature extraction method is based on both statistical and signal processing analyses. The feature selection method is based on statistical criteria. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=catenary%2Fpantograph%20interaction" title="catenary/pantograph interaction">catenary/pantograph interaction</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnostics" title=" diagnostics"> diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=Prognostics%20and%20Health%20Management%20%28PHM%29" title=" Prognostics and Health Management (PHM)"> Prognostics and Health Management (PHM)</a>, <a href="https://publications.waset.org/abstracts/search?q=quality%20of%20current%20collection" title=" quality of current collection"> quality of current collection</a> </p> <a href="https://publications.waset.org/abstracts/63877/pantograph-catenary-contact-force-features-evaluation-for-catenary-diagnostics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/63877.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">290</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">318</span> Fluorescent Ph-Sensing Bandage for Point-of-Care Wound Diagnostics</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Cherifi%20Katia">Cherifi Katia</a>, <a href="https://publications.waset.org/abstracts/search?q=Al-Hawat%20Marie-Lynn"> Al-Hawat Marie-Lynn</a>, <a href="https://publications.waset.org/abstracts/search?q=Tricou%20Leo-Paul"> Tricou Leo-Paul</a>, <a href="https://publications.waset.org/abstracts/search?q=Lamontagne%20Stephanie"> Lamontagne Stephanie</a>, <a href="https://publications.waset.org/abstracts/search?q=Tran%20Minh"> Tran Minh</a>, <a href="https://publications.waset.org/abstracts/search?q=Ngu%20Amy%20Ching%20Yie"> Ngu Amy Ching Yie</a>, <a href="https://publications.waset.org/abstracts/search?q=Manrique%20Gabriela"> Manrique Gabriela</a>, <a href="https://publications.waset.org/abstracts/search?q=Guirguis%20Natalie"> Guirguis Natalie</a>, <a href="https://publications.waset.org/abstracts/search?q=Machuca%20Parra%20Arturo%20Israel"> Machuca Parra Arturo Israel</a>, <a href="https://publications.waset.org/abstracts/search?q=Matoori%20Simon"> Matoori Simon</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Diabetic foot ulcers (DFUs) are a serious and prevalent complication of diabetes. Current diagnostic options are limited to macroscopic wound analysis such as wound size, depth, and infection. Molecular diagnostics promise to improve DFU diagnosis, staging, and assessment of treatment response. Here, we developed a rapid and easy-to-use fluorescent pH-sensing bandage for wound diagnostics. In a fluorescent dye screen, we identified pyranine as the lead compound due to its suitable pH-sensing properties in the clinically relevant pH range of 6 to 9. To minimize the release of this dye into the wound bed, we screened a library of ionic microparticles and found a strong adhesion of the anionic dye to a cationic polymeric microparticle. These dye-loaded microparticles showed a strong fluorescence response in the clinically relevant pH range of 6 to 9 and a dye release below 1% after one day in biological media. The dye-loaded microparticles were subsequently encapsulated in a calcium alginate hydrogel to minimize the interaction of the microparticles with the wound tissue. This pH-sensing diagnostic wound dressing was tested on full-thickness dorsal wounds of mice, and a linear fluorescence response (R2 = 0.9909) to clinically relevant pH values was observed. These findings encourage further development of this pH-sensing system for molecular diagnostics in DFUs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=wound%20ph" title="wound ph">wound ph</a>, <a href="https://publications.waset.org/abstracts/search?q=fluorescence" title=" fluorescence"> fluorescence</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnostics" title=" diagnostics"> diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=diabetic%20foot%20ulcer" title=" diabetic foot ulcer"> diabetic foot ulcer</a>, <a href="https://publications.waset.org/abstracts/search?q=wound%20healing" title=" wound healing"> wound healing</a>, <a href="https://publications.waset.org/abstracts/search?q=chronic%20wounds" title=" chronic wounds"> chronic wounds</a>, <a href="https://publications.waset.org/abstracts/search?q=diabetes" title=" diabetes"> diabetes</a> </p> <a href="https://publications.waset.org/abstracts/177542/fluorescent-ph-sensing-bandage-for-point-of-care-wound-diagnostics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/177542.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">86</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">317</span> Diagnostics of Subclinical Mastitis in Dairy Cows</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=G.%20Tanbayeva">G. Tanbayeva</a>, <a href="https://publications.waset.org/abstracts/search?q=Z.%20Myrzabekov"> Z. Myrzabekov</a>, <a href="https://publications.waset.org/abstracts/search?q=O.%20Tagayev"> O. Tagayev</a>, <a href="https://publications.waset.org/abstracts/search?q=B.%20Barakhov"> B. Barakhov</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Tokayeva"> M. Tokayeva</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mastitis is widely spread among dairy cows bringing large economic damage resulting in decreased milk yield, deterioration of the milk quality, gastrointestinal tract disorders among young animals, culling of breeding stock, and expenses for sick animal treatment. Up-to-date and accurate diagnostics of subclinical (latent) mastitis in dairy cows has huge practical and economical significance. The aim of the research was to develop a new optimal alternative rapid method for the diagnosis of subclinical mastitis in cows. The study was performed in the laboratory of the Hygiene and Sanitation of Kazakh National Agrarian University. The first stage was to evaluate the different percentages of “Promastit” preparation. It showed that the best diagnostics capacity had 10% dilution. The second stage was to compare “Promastit” with some of the domestic and foreign analogues “Somatic-Test” (Denmark), “MastTest” (Russia), “Mastidin” (Ukraine), “Diagmast” (Kazakhstan). The observation was carried out on 520 dairy cows with subclinical mastitis on farms of Almaty region of Kazakhstan. The effectiveness was checked by milk sedimentation test. Our research tends to show that the diagnostic test "Promastitis" revealed subclinical mastitis in 193 out of 520 lactating cows (37.1% of those examined). At the same time, in the case of using other diagnostic tests, the given index was as follows: 35.5% (mastidin), 34.4% (masttest-AF), 33.8% (somatic-test Ecotest), 30.7% (diagmast). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dairy%20cows" title="dairy cows">dairy cows</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnostics" title=" diagnostics"> diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=subclinical%20mastitis" title=" subclinical mastitis"> subclinical mastitis</a>, <a href="https://publications.waset.org/abstracts/search?q=test%20Promastit" title=" test Promastit"> test Promastit</a> </p> <a href="https://publications.waset.org/abstracts/43472/diagnostics-of-subclinical-mastitis-in-dairy-cows" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/43472.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">354</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">316</span> Diagnostics of Existing Steel Structures of Winter Sport Halls</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marcela%20Karmaz%C3%ADnov%C3%A1">Marcela Karmazínová</a>, <a href="https://publications.waset.org/abstracts/search?q=Jindrich%20Melcher"> Jindrich Melcher</a>, <a href="https://publications.waset.org/abstracts/search?q=Lubom%C3%ADr%20V%C3%ADtek"> Lubomír Vítek</a>, <a href="https://publications.waset.org/abstracts/search?q=Petr%20Cikrle"> Petr Cikrle</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The paper deals with the diagnostics of steel roof structure of the winter sports stadiums built in 1970 year. The necessity of the diagnostics has been given by the requirement to the evaluation design of this structure, which has been caused by the new situation in the field of the loadings given by the validity of the European Standards in the Czech Republic from 2010 year. Due to these changes in the normative rules, in practice, existing structures are gradually subjected to the evaluation design and depending on its results to the strengthening or reconstruction, respectively. The steel roof is composed of plane truss main girders, purlins and bracings and the roof structure is supported by two arch main girders with the span of L=84 m. The in situ diagnostics of the roof structure was oriented to the following parts: (i) determination and evaluation of the actual material properties of used steel and (ii) verification of the actual dimensions of the structural members. For the solution, the non-destructive methods have been used for in situ measurement. For the indicative determination of steel strengths the modified method based on the determination of Rockwell’s hardness has been used. For the verification of the member’s dimensions (thickness of hollow sections) the ultrasound method has been used. This paper presents the results obtained using these testing methods and their evaluation, from the viewpoint of the usage for the subsequent static assessment and design evaluation of the existing structure. For the comparison, the examples of the similar evaluations realized for steel structures of the stadiums in Olomouc and Jihlava cities are briefly illustrated, too. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=actual%20dimensions" title="actual dimensions">actual dimensions</a>, <a href="https://publications.waset.org/abstracts/search?q=destructive%20methods" title=" destructive methods"> destructive methods</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnostics" title=" diagnostics"> diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=existing%20steel%20structure" title=" existing steel structure"> existing steel structure</a>, <a href="https://publications.waset.org/abstracts/search?q=indirect%20non-destructive%20methods" title=" indirect non-destructive methods"> indirect non-destructive methods</a>, <a href="https://publications.waset.org/abstracts/search?q=Rockwel%E2%80%99s%20hardness" title=" Rockwel’s hardness"> Rockwel’s hardness</a>, <a href="https://publications.waset.org/abstracts/search?q=sport%20hall" title=" sport hall"> sport hall</a>, <a href="https://publications.waset.org/abstracts/search?q=steel%20strength" title=" steel strength"> steel strength</a>, <a href="https://publications.waset.org/abstracts/search?q=ultrasound%20method." title=" ultrasound method."> ultrasound method.</a> </p> <a href="https://publications.waset.org/abstracts/24272/diagnostics-of-existing-steel-structures-of-winter-sport-halls" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/24272.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">341</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">315</span> Highly-Sensitive Nanopore-Based Sensors for Point-Of-Care Medical Diagnostics </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Leyla%20Esfandiari">Leyla Esfandiari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Rapid, sensitive detection of nucleic acid (NA) molecules of specific sequence is of interest for a range of diverse health-related applications such as screening for genetic diseases, detecting pathogenic microbes in food and water, and identifying biological warfare agents in homeland security. Sequence-specific nucleic acid detection platforms rely on base pairing interaction between two complementary single stranded NAs, which can be detected by the optical, mechanical, or electrochemical readout. However, many of the existing platforms require amplification by polymerase chain reaction (PCR), fluorescent or enzymatic labels, and expensive or bulky instrumentation. In an effort to address these shortcomings, our research is focused on utilizing the cutting edge nanotechnology and microfluidics along with resistive pulse electrical measurements to design and develop a cost-effective, handheld and highly-sensitive nanopore-based sensor for point-of-care medical diagnostics. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diagnostics" title="diagnostics">diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=nanopore" title=" nanopore"> nanopore</a>, <a href="https://publications.waset.org/abstracts/search?q=nucleic%20acids" title=" nucleic acids"> nucleic acids</a>, <a href="https://publications.waset.org/abstracts/search?q=sensor" title=" sensor "> sensor </a> </p> <a href="https://publications.waset.org/abstracts/35912/highly-sensitive-nanopore-based-sensors-for-point-of-care-medical-diagnostics" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35912.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">464</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">314</span> Failure Analysis of the Gasoline Engines Injection System</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jozef%20Jurcik">Jozef Jurcik</a>, <a href="https://publications.waset.org/abstracts/search?q=Miroslav%20Gutten"> Miroslav Gutten</a>, <a href="https://publications.waset.org/abstracts/search?q=Milan%20Sebok"> Milan Sebok</a>, <a href="https://publications.waset.org/abstracts/search?q=Daniel%20Korenciak"> Daniel Korenciak</a>, <a href="https://publications.waset.org/abstracts/search?q=Jerzy%20Roj"> Jerzy Roj</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The paper presents the research results of electronic fuel injection system, which can be used for diagnostics of automotive systems. In the paper is described the construction and operation of a typical fuel injection system and analyzed its electronic part. It has also been proposed method for the detection of the injector malfunction, based on the analysis of differential current or voltage characteristics. In order to detect the fault state, it is needed to use self-learning process, by the use of an appropriate self-learning algorithm. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=electronic%20fuel%20injector" title="electronic fuel injector">electronic fuel injector</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnostics" title=" diagnostics"> diagnostics</a>, <a href="https://publications.waset.org/abstracts/search?q=measurement" title=" measurement"> measurement</a>, <a href="https://publications.waset.org/abstracts/search?q=testing%20device" title=" testing device"> testing device</a> </p> <a href="https://publications.waset.org/abstracts/23302/failure-analysis-of-the-gasoline-engines-injection-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/23302.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">552</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">313</span> Acute Phase Proteins, Proinflammatory Cytokines and Oxidative Stress Biomarkers in Sheep with Pneumonic Pasteurellosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Wael%20M.%20El-Deeb">Wael M. El-Deeb</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The aim of this study was to assess the pathophysiological importance of lipid profile, acute phase proteins, proinflammatory cytokines and oxidative stress markers in sheep with pneumonic pasteurellosis. Blood samples were collected from 36 Pasteurellamultocida-infected sheep, together with 20 healthy controls. Samples for bacteriological examination (nasal swabs, bronchoalveolar lavage) were collected from all animals and subjected to bacteriological examinations. Moreover, heart blood and lung samples were collected from the dead pneumonic sheep and subjected also to bacteriological examinations. A lipid profile was determined, along with a blood picture and other biochemical parameters. The acute phase proteins (fibrinogen, haptoglobin, serum amyloid A), the proinflammatory cytokine tumour necrosis factor-alpha, interleukins (IL-1α, IL-1β, IL-6), interferon-gamma and the oxidative stress markers malondialdehyde, super oxide dismutase, glutathione and catalase were also measured. The examined biochemical parameters were increased in the pneumonic sheep, except for cholesterol and high-density lipoprotein cholesterol (HDL-c), which were significantly lower than control group. Acute phase proteins and cytokines were significantly higher in the pneumonic sheep when compared to the healthy sheep. There was a significant increase in the levels of malondialdehyde; however, a significant decrease in the levels of super oxide dismutase, glutathione and catalase was observed. The present study shed the light on the possible pathphysiological role of lipid profile, acute phase proteins (APPs), proinflammatory cytokines and oxidative stress markers in pneumonic pasteurelosis in sheep. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acute%20phase%20proteins" title="acute phase proteins">acute phase proteins</a>, <a href="https://publications.waset.org/abstracts/search?q=sheep" title=" sheep"> sheep</a>, <a href="https://publications.waset.org/abstracts/search?q=pasteurella" title=" pasteurella"> pasteurella</a>, <a href="https://publications.waset.org/abstracts/search?q=interleukins" title=" interleukins"> interleukins</a>, <a href="https://publications.waset.org/abstracts/search?q=stress" title=" stress "> stress </a> </p> <a href="https://publications.waset.org/abstracts/40005/acute-phase-proteins-proinflammatory-cytokines-and-oxidative-stress-biomarkers-in-sheep-with-pneumonic-pasteurellosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/40005.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">391</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">312</span> Information System for Early Diabetic Retinopathy Diagnostics Based on Multiscale Texture Gradient Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=L.%20S.%20Godlevsky">L. S. Godlevsky</a>, <a href="https://publications.waset.org/abstracts/search?q=N.%20V.%20Kresyun"> N. V. Kresyun</a>, <a href="https://publications.waset.org/abstracts/search?q=V.%20P.%20Martsenyuk"> V. P. Martsenyuk</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20S.%20Shakun"> K. S. Shakun</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20V.%20Tatarchuk"> T. V. Tatarchuk</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20O.%20Prybolovets"> K. O. Prybolovets</a>, <a href="https://publications.waset.org/abstracts/search?q=L.%20F.%20Kalinichenko"> L. F. Kalinichenko</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Karpinski"> M. Karpinski</a>, <a href="https://publications.waset.org/abstracts/search?q=T.%20Gancarczyk"> T. Gancarczyk </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Structures of eye bottom were extracted using multiscale texture gradient method and color characteristics of macular zone and vessels were verified in CIELAB scale. The difference of average values of L*, a* and b* coordinates of CIE (International Commision of Illumination) scale in patients with diabetes and healthy volunteers was compared. The average value of L* in diabetic patients exceeded such one in the group of practically healthy persons by 2.71 times (<em>P</em>&nbsp;&lt;&nbsp;0.05), while the value of a* index was reduced by 3.8 times when compared with control one (<em>P</em>&nbsp;&lt;&nbsp;0.05). b* index exceeded such one in the control group by 12.4 times (<em>P</em>&nbsp;&lt;&nbsp;0.05). The integrated index on color difference (&Delta;<em>E</em>) exceeded control value by 2.87 times (<em>P</em>&nbsp;&lt;&nbsp;0.05). More pronounced differences with &Delta;<em>E</em> were followed by a shorter period of MA appearance with a correlation level at -0.56 (<em>P</em>&nbsp;&lt;&nbsp;0.05). The specificity of diagnostics raised by 2.17 times (<em>P</em>&nbsp;&lt;&nbsp;0.05) and negative prognostic index exceeded such one determined with the expert method by 2.26 times (<em>P</em>&nbsp;&lt;&nbsp;0.05). <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diabetic%20retinopathy" title="diabetic retinopathy">diabetic retinopathy</a>, <a href="https://publications.waset.org/abstracts/search?q=multiscale%20texture%20gradient" title=" multiscale texture gradient"> multiscale texture gradient</a>, <a href="https://publications.waset.org/abstracts/search?q=color%20spectrum%20analysis" title=" color spectrum analysis"> color spectrum analysis</a>, <a href="https://publications.waset.org/abstracts/search?q=medical%20diagnostics" title=" medical diagnostics"> medical diagnostics</a> </p> <a href="https://publications.waset.org/abstracts/116592/information-system-for-early-diabetic-retinopathy-diagnostics-based-on-multiscale-texture-gradient-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/116592.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">115</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">311</span> Plasmonic Nanoshells Based Metabolite Detection for in-vitro Metabolic Diagnostics and Therapeutic Evaluation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Deepanjali%20Gurav">Deepanjali Gurav</a>, <a href="https://publications.waset.org/abstracts/search?q=Kun%20Qian"> Kun Qian</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In-vitro metabolic diagnosis relies on designed materials-based analytical platforms for detection of selected metabolites in biological samples, which has a key role in disease detection and therapeutic evaluation in clinics. However, the basic challenge deals with developing a simple approach for metabolic analysis in bio-samples with high sample complexity and low molecular abundance. In this work, we report a designer plasmonic nanoshells based platform for direct detection of small metabolites in clinical samples for in-vitro metabolic diagnostics. We first synthesized a series of plasmonic core-shell particles with tunable nanoshell structures. The optimized plasmonic nanoshells as new matrices allowed fast, multiplex, sensitive, and selective LDI MS (Laser desorption/ionization mass spectrometry) detection of small metabolites in 0.5 μL of bio-fluids without enrichment or purification. Furthermore, coupling with isotopic quantification of selected metabolites, we demonstrated the use of these plasmonic nanoshells for disease detection and therapeutic evaluation in clinics. For disease detection, we identified patients with postoperative brain infection through glucose quantitation and daily monitoring by cerebrospinal fluid (CSF) analysis. For therapeutic evaluation, we investigated drug distribution in blood and CSF systems and validated the function and permeability of blood-brain/CSF-barriers, during therapeutic treatment of patients with cerebral edema for pharmacokinetic study. Our work sheds light on the design of materials for high-performance metabolic analysis and precision diagnostics in real cases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=plasmonic%20nanoparticles" title="plasmonic nanoparticles">plasmonic nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=metabolites" title=" metabolites"> metabolites</a>, <a href="https://publications.waset.org/abstracts/search?q=fingerprinting" title=" fingerprinting"> fingerprinting</a>, <a href="https://publications.waset.org/abstracts/search?q=mass%20spectrometry" title=" mass spectrometry"> mass spectrometry</a>, <a href="https://publications.waset.org/abstracts/search?q=in-vitro%20diagnostics" title=" in-vitro diagnostics"> in-vitro diagnostics</a> </p> <a href="https://publications.waset.org/abstracts/91905/plasmonic-nanoshells-based-metabolite-detection-for-in-vitro-metabolic-diagnostics-and-therapeutic-evaluation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/91905.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">138</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">310</span> Condition Based Assessment of Power Transformer with Modern Techniques</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Piush%20Verma">Piush Verma</a>, <a href="https://publications.waset.org/abstracts/search?q=Y.%20R.%20Sood"> Y. R. Sood</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This paper provides the information on the diagnostics techniques for condition monitoring of power transformer (PT). This paper deals with the practical importance of the transformer diagnostic in the Electrical Engineering field. The life of the transformer depends upon its insulation i.e paper and oil. The major testing techniques applies on transformer oil and paper i.e dissolved gas analysis, furfural analysis, radio interface, acoustic emission, infra-red emission, frequency response analysis, power factor, polarization spectrum, magnetizing currents, turn and winding ratio. A review has been made on the modern development of this practical technology. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=temperature" title="temperature">temperature</a>, <a href="https://publications.waset.org/abstracts/search?q=condition%20monitoring" title=" condition monitoring"> condition monitoring</a>, <a href="https://publications.waset.org/abstracts/search?q=diagnostics%20methods" title=" diagnostics methods"> diagnostics methods</a>, <a href="https://publications.waset.org/abstracts/search?q=paper%20analysis%20techniques" title=" paper analysis techniques"> paper analysis techniques</a>, <a href="https://publications.waset.org/abstracts/search?q=oil%20analysis%20techniques" title=" oil analysis techniques"> oil analysis techniques</a> </p> <a href="https://publications.waset.org/abstracts/46784/condition-based-assessment-of-power-transformer-with-modern-techniques" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/46784.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">433</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">309</span> Physicochemical and Bacteriological Assessment of Water Resources in Ughelli and Its Environs, Delta State Nigeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20O.%20Eyankware">M. O. Eyankware</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20O.%20Ufomata"> D. O. Ufomata </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Groundwater samples were collected from Otovwodo-Ughelli and Environ with the aim of assessing groundwater quality of the area. Twenty (20) water samples from Boreholes (BH) (six) and Hand Dug Wells (HDW) (fourteen) were randomly sampled and were analysed for different physiochemical and bacteriological parameters. The following 16 parameters have been considered viz: pH, electrical conductivity, temperature, total hardness, total dissolved solids, dissolved oxygen, biological oxygen demand, phosphate, sulphate, chloride, nitrate, calcium, sodium, chloride, magnesium, and total suspended solids. On comparing the results against drinking quality standards laid by World Health Organization and Nigeria industrial standard, it was found that the water quality parameters were not above the (WHO, 2011 and NIS, 2007) permissible limit. Microbial analysis reveals the presence of coliform and E.coli in two hand-dug well (HDW7 and 13) and one borehole well (BH20). These contaminations are perhaps traceable to have originated from human activities (septic tanks, latrines, dumpsites) and have affected the quality of groundwater in Otovwodo-Ughelli. From the piper trilinear diagram, the dominant ionic species is alkali bicarbonate water type, with bicarbonate as the predominant ion (Na+ + K+)-HCO3. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=groundwater" title="groundwater">groundwater</a>, <a href="https://publications.waset.org/abstracts/search?q=surface%20water" title=" surface water"> surface water</a>, <a href="https://publications.waset.org/abstracts/search?q=Ughelli" title=" Ughelli"> Ughelli</a>, <a href="https://publications.waset.org/abstracts/search?q=Nigeria%20industrial%20standard" title=" Nigeria industrial standard"> Nigeria industrial standard</a>, <a href="https://publications.waset.org/abstracts/search?q=who%20standard" title=" who standard"> who standard</a> </p> <a href="https://publications.waset.org/abstracts/30654/physicochemical-and-bacteriological-assessment-of-water-resources-in-ughelli-and-its-environs-delta-state-nigeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/30654.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">444</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">308</span> Bacteriological Safety of Sachet Drinking Water Sold in Benin City, Nigeria</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Stephen%20Olusanmi%20Akintayo">Stephen Olusanmi Akintayo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Access to safe drinking water remains a major challenge in Nigeria, and where available, the quality of the water is often in doubt. An alternative to the inadequate clean drinking water is being found in treated drinking water packaged in electrically heated sealed nylon and commonly referred to as “sachet water”. “Sachet water” is a common thing in Nigeria as the selling price is within the reach of members of the low socio- economic class and the setting up of a production unit does not require huge capital input. The bacteriological quality of selected “sachet water” stored at room temperature over a period of 56 days was determined to evaluate the safety of the sachet drinking water. Test for the detection of coliform bacteria was performed, and the result showed no coliform bacteria that indicates the absence of fecal contamination throughout 56 days. Heterotrophic plate count (HPC) was done at an interval 14 days, and the samples showed HPC between 0 cfu/mL and 64 cfu/mL. The highest count was observed on day 1. The count decreased between day 1 and 28, while no growths were observed between day 42 and 56. The decrease in HPC suggested the presence of residual disinfectant in the water. The organisms isolated were identified as Staphylococcus epidermis and S. aureus. The presence of these microorganisms in sachet water is indicative for contamination during processing and handling. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=coliform" title="coliform">coliform</a>, <a href="https://publications.waset.org/abstracts/search?q=heterotrophic%20plate%20count" title=" heterotrophic plate count"> heterotrophic plate count</a>, <a href="https://publications.waset.org/abstracts/search?q=sachet%20water" title=" sachet water"> sachet water</a>, <a href="https://publications.waset.org/abstracts/search?q=Staphyloccocus%20aureus" title=" Staphyloccocus aureus"> Staphyloccocus aureus</a>, <a href="https://publications.waset.org/abstracts/search?q=Staphyloccocus%20epidermidis" title=" Staphyloccocus epidermidis"> Staphyloccocus epidermidis</a> </p> <a href="https://publications.waset.org/abstracts/77952/bacteriological-safety-of-sachet-drinking-water-sold-in-benin-city-nigeria" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/77952.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">341</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">307</span> Peculiarities of Microflora of Odontogenic Inflammatory Processes in the Central Kazakhstan Region</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aliya%20Tokbergenova">Aliya Tokbergenova</a>, <a href="https://publications.waset.org/abstracts/search?q=Maida%20Tusupbekova"> Maida Tusupbekova</a>, <a href="https://publications.waset.org/abstracts/search?q=Daulet%20Dzhangaliyev"> Daulet Dzhangaliyev</a>, <a href="https://publications.waset.org/abstracts/search?q=Alena%20Lavrinenko"> Alena Lavrinenko</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Odontogenic phlegmons are ranked the first among pyoinflammatory processes in the frequency of hospitalization in maxillofacial surgery in the post-Soviet countries. The main role in etiology is played by obligate anaerobes and aerobes. According to numerous data, the structure of aerobic pathogens is dominated by staphylococci and gram-negative bacteria. Aim: The research aim is to study the microflora of the purulent discharge odontogenic inflammatory processes. Materials and methods: A total of 220 patients have been examined, of which 120 patients aged 25-59 years have been included in the research who did not have comorbidity hospitalized in the maxillofacial hospital in Karaganda (Kazakhstan) from January 2016 to July 2017. The bacteriological research has been carried out on the basis of the multiaccess laboratory of the KSMU, through the Matrix Assisted Laser Desorption/Ionization (MALDI) apparatus. The material sample was pus from the inflammation focus, taken during the operating period. Results: According to the research among 120 patients (100%), 15 patients (12.5%) have had microorganisms not grown. From 105 (87.5%) bacteriological results, it has been revealed the following 1) Streptococcus: 51 (42.5%): Streptococcus beta-haemolytic: 17 (14.2%), Streptococcus pneumoniae: 12 (10%), Streptococcus anginosus: 8 (6.6%), Streptococcus oralis: 8 (6.6%), Streptococcus constellatus: 6 (5.0%); 2) Staphylococci: 27 (22.5%): Staphylococci aureus: 14 (11.7%) and Staphylococci epidermidis: 13 (10.8%); 3) Pseudomonas aeruginosa: 12 (10%); 4) Neisseria: 11 (9.1%): Neisseria mucosa: 5 (4.1%) and Neisseria macacae: 6 (5.0%); 5) Klebsiella pneumoniae: 2 (1.7%); 6) Stenotrophomonas maltophilia: 2 (1.7%). 15 patients (12.5%) experienced complications in the form of 1) The dissemination of the process in 10 patients (8.4%). 2) Osteomyelitis in 3 (2.5%). 3) Mediastinitis in 1 (0.8%). 4) Sinusitis in 1 (0.8%). 15 patients (100%) were carried out repeated bacteriological examination, the following was revealed: 1) Streptococcus: 10 (66.7%): Streptococcus beta-haemolytic: 4 (26.7%), Streptococcus pneumoniae: 2 (13.3%), Streptococcus аnginosus: 2 (13.3%), Streptococcus oralis: 1 (6.7%), Streptococcus constellatus: 1 (6.7%); 2) Staphylococci: 4 (26.7%): Staphylococci aureus: 3 (20%) and Staphylococci epidermidis: 1 (6.7%); 3) Pseudomonas aeruginosa: 1 (6.7%). Conclusions: Thus, according to our research data, streptococci predominate in the odontogenic processes microflora in aerobic flora in the central Kazakhstan region, which refutes the leading role of staphylococci in the development of odontogenic inflammatory processes, thus creating prerequisites for studying new treatment approaches. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=maxillofacial%20surgery" title="maxillofacial surgery">maxillofacial surgery</a>, <a href="https://publications.waset.org/abstracts/search?q=microflora" title=" microflora"> microflora</a>, <a href="https://publications.waset.org/abstracts/search?q=odontogenic%20phlegmons" title=" odontogenic phlegmons"> odontogenic phlegmons</a>, <a href="https://publications.waset.org/abstracts/search?q=pyo-inflammatory" title=" pyo-inflammatory"> pyo-inflammatory</a> </p> <a href="https://publications.waset.org/abstracts/91569/peculiarities-of-microflora-of-odontogenic-inflammatory-processes-in-the-central-kazakhstan-region" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/91569.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">193</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">306</span> Clustered Regularly Interspaced Short Palindromic Repeat/cas9-Based Lateral Flow and Fluorescence Diagnostics for Rapid Pathogen Detection</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mark%20Osborn">Mark Osborn</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Clustered, regularly interspaced short palindromic repeat (CRISPR/Cas) proteins can be designed to bind specified DNA and RNA sequences and hold great promise for the accurate detection of nucleic acids for diagnostics. Commercially available reagents were integrated into a CRISPR/Cas9-based lateral flow assay that can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequences with single-base specificity. This approach requires minimal equipment and represents a simplified platform for field-based deployment. A rapid, multiplex fluorescence CRISPR/Cas9 nuclease cleavage assay capable of detecting and differentiating SARS-CoV-2, influenza A and B, and respiratory syncytial virus in a single reaction was also developed. These findings provide proof of principle for CRISPR/Cas9 point-of-care diagnosis that can detect specific SARS-CoV-2 strain(s). Further, Cas9 cleavage allows for a scalable fluorescent platform for identifying respiratory viral pathogens with overlapping symptomology. Collectively, this approach is a facile platform for diagnostics with broad application to user-defined sequence interrogation and detection. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=CRISPR%2FCas9" title="CRISPR/Cas9">CRISPR/Cas9</a>, <a href="https://publications.waset.org/abstracts/search?q=lateral%20flow%20assay" title=" lateral flow assay"> lateral flow assay</a>, <a href="https://publications.waset.org/abstracts/search?q=SARS-Co-V2" title=" SARS-Co-V2"> SARS-Co-V2</a>, <a href="https://publications.waset.org/abstracts/search?q=single-nucleotide%20resolution" title=" single-nucleotide resolution"> single-nucleotide resolution</a> </p> <a href="https://publications.waset.org/abstracts/134880/clustered-regularly-interspaced-short-palindromic-repeatcas9-based-lateral-flow-and-fluorescence-diagnostics-for-rapid-pathogen-detection" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/134880.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">184</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">305</span> Using Scanning Electron Microscope and Computed Tomography for Concrete Diagnostics of Airfield Pavements</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=M.%20Linek">M. Linek</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This article presents the comparison of selected evaluation methods regarding microstructure modification of hardened cement concrete intended for airfield pavements. Basic test results were presented for two pavement quality concrete lots. Analysis included standard concrete used for airfield pavements and modern material solutions based on concrete composite modification. In case of basic grain size distribution of concrete cement CEM I 42,5HSR NA, fine aggregate and coarse aggregate fractions in the form of granite chippings, water and admixtures were considered. In case of grain size distribution of modified concrete, the use of modern modifier as substitute of fine aggregate was suggested. Modification influence on internal concrete structure parameters using scanning electron microscope was defined. Obtained images were compared to the results obtained using computed tomography. Opportunity to use this type of equipment for internal concrete structure diagnostics and an attempt of its parameters evaluation was presented. Obtained test results enabled to reach a conclusion that both methods can be applied for pavement quality concrete diagnostics, with particular purpose of airfield pavements. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=scanning%20electron%20microscope" title="scanning electron microscope">scanning electron microscope</a>, <a href="https://publications.waset.org/abstracts/search?q=computed%20tomography" title=" computed tomography"> computed tomography</a>, <a href="https://publications.waset.org/abstracts/search?q=cement%20concrete" title=" cement concrete"> cement concrete</a>, <a href="https://publications.waset.org/abstracts/search?q=airfield%20pavements" title=" airfield pavements"> airfield pavements</a> </p> <a href="https://publications.waset.org/abstracts/53038/using-scanning-electron-microscope-and-computed-tomography-for-concrete-diagnostics-of-airfield-pavements" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/53038.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">339</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">304</span> Epidemiological, Clinical and Bacteriological Profile of Human Brucellosis in the District of Tunis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jihene%20Bettaieb">Jihene Bettaieb</a>, <a href="https://publications.waset.org/abstracts/search?q=Ghassen%20kharroubi"> Ghassen kharroubi</a>, <a href="https://publications.waset.org/abstracts/search?q=Rym%20mallekh"> Rym mallekh</a>, <a href="https://publications.waset.org/abstracts/search?q=Ines%20Cherif"> Ines Cherif</a>, <a href="https://publications.waset.org/abstracts/search?q=Taoufik%20Atawa"> Taoufik Atawa</a>, <a href="https://publications.waset.org/abstracts/search?q=Kaouther%20Harrabech"> Kaouther Harrabech</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Brucellosis is a major worldwide zoonosis. It is a reportable condition in Tunisia where the disease remains endemic, especially in rural areas. The aim of this study was to describe the epidemiological, clinical, and bacteriological profile of human brucellosis cases notified in the district of Tunis. It was a retrospective descriptive study of cases reported in the district of Tunis through the national surveillance system between the 1st January and 31th December 2017. During the study period, 133 brucellosis confirmed cases were notified. The mean age was 37.5 ± 18.0 years, and 54.9% of cases were males. More than four-fifths (82.7%) of cases were reported in spring and summer with a peak in the month of May (36 cases). Fever and sweats were the most common symptoms; they occurred in 95% and 72% of cases, respectively. Osteoarticular complications occurred in 10 cases, meningitis in one case and endocarditis in one other case. Wright agglutination test and Rose Bengale test were positive in 100% and 91% of cases, respectively. While blood culture was positive in 9 cases and PCR in 2 cases. Brucella melitensis was the only identified specie (9 cases). Almost all cases (99.2%) reported the habit of consuming raw dairy products. Only 5 cases had a suspect contact with animals; among them, 3 persons were livestock breeders. The transmission was essentially due to raw dairy product consumption. It is important to enhance preventive measures to control animal Brucellosis and to educate the population regarding the risk factors of the disease. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=brucellosis" title="brucellosis">brucellosis</a>, <a href="https://publications.waset.org/abstracts/search?q=risk%20factors" title=" risk factors"> risk factors</a>, <a href="https://publications.waset.org/abstracts/search?q=surveillance%20system" title=" surveillance system"> surveillance system</a>, <a href="https://publications.waset.org/abstracts/search?q=Tunisia" title=" Tunisia"> Tunisia</a> </p> <a href="https://publications.waset.org/abstracts/127936/epidemiological-clinical-and-bacteriological-profile-of-human-brucellosis-in-the-district-of-tunis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/127936.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">135</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">303</span> Laser Ultrasonic Diagnostics and Acoustic Emission Technique for Examination of Rock Specimens under Uniaxial Compression</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Elena%20B.%20Cherepetskaya">Elena B. Cherepetskaya</a>, <a href="https://publications.waset.org/abstracts/search?q=Vladimir%20A.%20Makarov"> Vladimir A. Makarov</a>, <a href="https://publications.waset.org/abstracts/search?q=Dmitry%20V.%20Morozov"> Dmitry V. Morozov</a>, <a href="https://publications.waset.org/abstracts/search?q=Ivan%20E.%20Sas"> Ivan E. Sas</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Laboratory studies of the stress-strain behavior of rocks specimens were conducted by using acoustic emission and laser-ultrasonic diagnostics. The sensitivity of the techniques allowed changes in the internal structure of the specimens under uniaxial compressive load to be examined at micro- and macro scales. It was shown that microcracks appear in geologic materials when the stress level reaches about 50% of breaking strength. Also, the characteristic stress of the main crack formation was registered in the process of single-stage compression of rocks. On the base of laser-ultrasonic echoscopy, 2D visualization of the internal structure of rocky soil specimens was realized, and the microcracks arising during uniaxial compression were registered. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=acoustic%20emission" title="acoustic emission">acoustic emission</a>, <a href="https://publications.waset.org/abstracts/search?q=geomaterial" title=" geomaterial"> geomaterial</a>, <a href="https://publications.waset.org/abstracts/search?q=laser%20ultrasound" title=" laser ultrasound"> laser ultrasound</a>, <a href="https://publications.waset.org/abstracts/search?q=uniaxial%20compression" title=" uniaxial compression"> uniaxial compression</a> </p> <a href="https://publications.waset.org/abstracts/54531/laser-ultrasonic-diagnostics-and-acoustic-emission-technique-for-examination-of-rock-specimens-under-uniaxial-compression" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/54531.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">375</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">302</span> Drinking Water Quality of Lahore Pakistan: A Comparison of Quality of Drinking Water from Source and Distribution System</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zainab%20Abbas%20Soharwardi">Zainab Abbas Soharwardi</a>, <a href="https://publications.waset.org/abstracts/search?q=Chunli%20Su"> Chunli Su</a>, <a href="https://publications.waset.org/abstracts/search?q=Fazeelat%20Tahira"> Fazeelat Tahira</a>, <a href="https://publications.waset.org/abstracts/search?q=Syed%20Zahid%20Aziz"> Syed Zahid Aziz</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The study monitors the quality of drinking water consumed by urban population of Lahore. A total of 50 drinking water samples (16 from source and 34 from distribution system) were examined for physical, chemical and bacteriological parameters. The parameters including pH, turbidity, electrical conductivity, total dissolved solids, total hardness, calcium, magnesium, total alkalinity, carbonate, sulphate, chloride, nitrite, fluoride, sodium and potassium were analyzed. Sixteen out of fifty samples showed high values of alkalinity compared to EPA standards and WHO guidelines. Twenty-eight samples were analyzed for heavy metals, chromium, iron, copper, zinc, cadmium and lead. Trace amounts of heavy metals were detected in some samples, however for most of the samples values were within the permissible limits although high concentration of zinc was detected in one sample collected from Mughal Pura area. Fifteen samples were analyzed for arsenic. The results were unsatisfactory; around 73% samples showed exceeding values of As. WHO has suggested permissible limits of arsenic < 0.01 ppm, whereas 27 % of samples have shown 0.05 ppm arsenic, which is five times greater than WHO highest permissible limits. All the samples were examined for E. coli bacteria. On the basis of bacteriological analysis, 42 % samples did not meet WHO guidelines and were unsafe for drinking. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=arsenic" title="arsenic">arsenic</a>, <a href="https://publications.waset.org/abstracts/search?q=heavy%20metals" title=" heavy metals"> heavy metals</a>, <a href="https://publications.waset.org/abstracts/search?q=ground%20water" title=" ground water"> ground water</a>, <a href="https://publications.waset.org/abstracts/search?q=Lahore" title=" Lahore"> Lahore</a> </p> <a href="https://publications.waset.org/abstracts/42495/drinking-water-quality-of-lahore-pakistan-a-comparison-of-quality-of-drinking-water-from-source-and-distribution-system" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/42495.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads 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