Rare Cell Isolation | Bio-Techne

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<div class="highlighted"> <aside class="container section clearfix" role="complementary"> <div> </div> </aside> </div> <div id="main" class="container-fluid container-wide "> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> <a href="/resources">Resources</a> </li> <li> <a href="/resources/instrument-applications">ProteinSimple Instrument Applications</a> </li> <li> Rare Cell Isolation </li> </ol> </nav> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <article> <div> <div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h1>Rare Cell Isolation</h1></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h3>Fast & Gentle Rare Cell Isolation</h3> <ul> <li><a href="#enrichment">Rare Cell Enrichment with Bio-Techne's Cell Sorter</a></li> <li><a href="#benefits">Benefits of the Bio-Techne’s Single Cell Sorter</a></li> <li><a href="#isolating">Isolating Rare Cells using the Bio-Techne’s cell sorter</a></li> <li><a href="#resources">Rare Cell Isolation Resources</a></li> <li><a href="#testimonials">Testimonials from Your Peers</a></li> </ul> <p> </p></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_call_to_action_global"> <a href="#quote" class="btn btn-primary" type="link"> Request Information </a> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <a href="https://www.bio-techne.com#quote" title="Request Information" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/about/v2_AAI-LP-new-Pala.png" alt="Pala Cell Sorter and Single Cell Dispenser hero image" loading="lazy" > </a> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-media-to-text mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_indv_media"> <div> <a href="https://www.bio-techne.com#quote" title="Request Demo, Pricing or more Info" > <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/enhrichment-cell-sorting-v3.jpg" alt="Enrichment Cell Sorting Figure" loading="lazy" > </a> </div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_media_to_text:field_header_2_global"> <div><h2>Rare Cell Enrichment with Bio-Techne's Cell Sorting and Single Cell Dispensing Technology</h2></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_lg_sngl_frmtd_text_global"> <div><p><a name="enrichment"></a>Rare cells are typically defined as cells that exist at very low concentrations (<0.1%) in a large background population. Circulating Tumor Cells (CTCs) and Circulating Fetal Cells (CFCs) in blood samples are two rare cell types that hold great therapeutic potential. Isolating rare cells has traditionally relied on enrichment methods such as filtration and magnetic bead selection, however, these techniques lack specificity and often yield mixed results.<br> <br> <a href="/instruments/single-cell-dispensers">Bio-Techne's cell sorting technology</a> enables for gentle, rare cell enrichment utilizing fluorescence.<br> <br> Utilizing the "enrichment" workflow paired with single cell dispensing will allow for the isolation of rare cells for downstream analysis or use.<br> <br> A typical workflow would start with labeling rare target cells with fluorescence tags followed by loading the high-density samples into a <a href="/p/cell-sorting-and-single-cell-dispensing/single-cell-cartridge-kit_nc003">single cell</a> or <a href="/p/cell-sorting-and-single-cell-dispensing/bulk-sorting-cartridge-kit_nc101">bulk cartridge</a>. Cells with the specific fluorescence profile are gated and a fluorescence "trigger" is set to ignore cells below the threshold. When a cell with </p></div> </div> <div class="field_block:paragraph:2_2_media_to_text:field_call_to_action_global"> <a href="#quote" class="btn btn-primary" type="link"> Request Demo, Pricing or more Info </a> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><a name="benefits"></a><h2>Benefits of Bio-Techne Cell Sorter and Single Cell Dispenser</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><h4>Gentle</h4> <p>Gentle sorting (<2 psi) preserves cell viability; higher clonality; better outgrowth</p> <h4>Easy</h4> <p>Quick start up in under 5 minutes with no calibration and minimal maintenance</p> <h4>Fast</h4> <p>Quickly isolate rare antigen-specific B cells; process up to 1M cells in 5 min</p> <h4>Sterile</h4> <p>Fits in a standard tissue culture hood; sterile and disposable cell cartridges eliminate sample to sample carryover</p> <h4>Efficient</h4> <p>Dispensing efficiency of 80-90% for CHO cells compared to ~30% with manual pipetting (percentage of dispensed wells with single cells - see figure right)</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/dispensing-efficiency-bar-graph-calibration-particles.jpg" alt="Bar chart highlighting the dispensing efficiency of Bio-Techne's single cell dispensers" loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--_-2-text-to-media mb-4 paragraph--view-mode--default"> <div class="container-fluid container-wide"> <div class="layout layout--twocol-section layout--twocol-section--50-50"> <div class="layout__region layout__region--first"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_header_2_global"> <div><h2>Isolating Rare Cells with the Bio-Techne Cell Sorter</h2></div> </div> <div class="field_block:paragraph:2_2_text_to_media:field_lg_sngl_frmtd_text_global"> <div><p><a name="isolating"></a>The Bio-Techne cell sorting technology was used to isolate EpCAM+ cancer cells spiked into a population of white blood cells at a frequency of 0.01%.</p> <p>CNV (copy number variation) across the genome were profiled and the results confirmed a cancer-characteristic CNV profile from the isolated EpCAM+ cells (top panel), as compared with the background cell population (bottom panel).</p> <p>Internal controls were carried out: the spiked-in cancer cells were female, carrying the XX genes, while the background cell population were male (XY genes). CNV analysis of the X and Y chromosomes supported that successful isolation of the EpCAM+ cancer cells had occurred.</p></div> </div> </div> </div> <div class="layout__region layout__region--second"> <div class="hero-content-wrap"> <div class="field_block:paragraph:2_2_text_to_media:field_indv_media"> <div> <img src="https://resources.bio-techne.com/bio-techne-assets/images/applications/isolating-rare-cells.jpg" alt="isolating rare cells panel with Bio-Techne's cell sorter " loading="lazy" > </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--section paragraph--view-mode--default mb-4"> <div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="resources"></a></p></div> </div> </div> <div> <div class="paragraph paragraph--type--header"> <div> <div><h2>Related Cell Sorter & Dispenser Resources</h2></div> </div> </div> </div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/resources/literature/rare-cell-isolation-with-single-cell-dispenser" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/research-areas/rare-cell-isolation-thumbnail-500x650.png" alt="In this application note, learn how Bio-Techne's microfluidic single cell dispenser effectively preserves the viability and integrity of rare cells like circulating tumor cells or fetal cells during isolation. " loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/rare-cell-isolation-with-single-cell-dispenser" > <h3 class="card_title_htag"> <div><h3>Application Notes</h3></div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/rare-cell-isolation-with-single-cell-dispenser" > Rare Cell Isolation </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/resources/literature/single-cell-deposition-efficiency" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/docs/literature/single-cell-deposition-efficiency-thumb.png" alt="Single Cell Deposition Efficiency App Note" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/literature/single-cell-deposition-efficiency" > <h3 class="card_title_htag"> <div><h3>Application Notes</h3></div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/literature/single-cell-deposition-efficiency" > Single Cell Deposition Efficiency </a> </div> </div> </div> </div> </div> <div class="col-md-4"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="/resources/webinars/view-all-webinars?tid_1%5B936%5D=936" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/icons/webinar-icon-blue-light.png" alt="On Demand Webinar Icon" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="/resources/webinars/view-all-webinars?tid_1%5B936%5D=936" > <h3 class="card_title_htag"> <div><h3>Cell Sorter Webinars</h3></div> </h3> </a> <div class="card_description flex-grow-1"> </div> <div class="card-bottom-link"> <div> <a href="/resources/webinars/view-all-webinars?tid_1%5B936%5D=936" > View All Cell Sorter Webinars </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div> <div class="paragraph paragraph--type--freeform mb-4 paragraph--view-mode--default"> <div><p><a name="testimonials"></a></p></div> </div> </div> <div> <div class="testimonial"> <div > <div class="row"> <div class="col-md-4"> <div class="left-title"> <h2>Customer Success Stories</h2> </div> </div> <div class="col-md-8"> <div class="right-card bg-white border rounded p-5 mb-4"> <div class="carousel slide" id="carouselIndicators" data-ride="carousel"> <div class="row"> <div class="col-md-12"> <div class="carousel-inner"> <div class="carousel-item active">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-eaffc930db1" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/logos/cytovance-biologic-logo.png' alt='Cytovance Biologic logo'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Mike Brem </span><br/> <span class="individual-testimonial__position"> </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> Here at Cytovance, we love the Bio-Techne Cell Sorter. This machine has increased our planning capacity and efficiency nearly four-fold. The accuracy of the Bio-Techne Cell Sorter's 384-well plating coupled with the easy changes between samples hs been a game changer for our workflow.  </div> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-286b4582747" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/logos/genentech-logo.png' alt='Genetech Logo'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Mandy Yim </span><br/> <span class="individual-testimonial__position"> </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> Bio-Techne's Cell Sorter & dispenser provides a great solution for gentle single cell cloning using sterile disposable cartridges. THe system is easy to use and quick to initialize. Hana combines th eadvantages of FACS and microfluidic cell dispensing to enable high assurance of monoclonality with high throughput and great cell viability.  </div> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-ae533c002f9" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/logos/c4therapeutic-logo.png' alt='C4 Therapeutics logo'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Trang Dickson </span><br/> <span class="individual-testimonial__position"> </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> Bio-Techne's disposable cartridges have allowed us to increase the throughput of the validation of our CRISPR engineered cell lines since we can sort a full plate in less than 3 minutes, and can move to the next plate without the hassle of cleaning. This outstanding product is paired with Bio-Techne's exceptional customer service that has been responsive to all our needs. While evaluating different FACS machines, we simply did not find a rival that combined Bio-techne's simplicity, performance and value, all while occupying a small footprint.  </div> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-2c613d1c2be" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/logos/gladstone-logo.png' alt='Gladstone Institutes logo'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Po-Lin So </span><br/> <span class="individual-testimonial__position"> </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> Our stem cell core facility was looking for a way to speed up our human induced pluripotent cell cloning to generate clonal cell lines, and to analyze our gene editing efficiencies in single cells. Our criteria for an instrument for single cell dispensing was: Sterility Gentle on cells Ease of use Reasonable cost The Bio-Techne's Cell Sorter & dispenser fitted our criteria perfectly. We are able to reduce the time and effort for generating clonal CRISPR-engineered iPSC lines significantly. The instrument requires very little maintenance and users do not need to be highly trained with the main requirement being aseptic technique. we are excited to use the Bio-Techne Cell sorter's potential for other single cell applications.  </div> </div> </div> </div> </div> <div class="carousel-item">  <div class="paragraph paragraph--type--individual-testimonial individual-testimonial paragraph--view-mode--default"> <div class="testimonial-info-group"> <div class="row"> <div class="col-lg-3 offset-lg-1 col-sm-12"> <div class="text-center text-lg-left"> <div class="individual-testimonial__headshot"> <div class="blazy blazy--field blazy--field-indv-img blazy--field-indv-img--default" data-blazy=""> <div data-b-token="b-c7724a9655d" class="media media--blazy media--rendered media--resourcesbio-techne media--bundle--remote-image"><img src='https://resources.bio-techne.com/bio-techne-assets/images/logos/biomicro-center-logo.png' alt='BioMicro Center logo'> </div> </div> </div> </div> </div> <div class="col-10 offset-1 col-sm-10 offset-sm-1 col-md-8 offset-md-2 col-lg-6 offset-lg-0"> <div class="testimonial-info"> <div class="individual-testimonial__info"> <span class="individual-testimonial__name"> Noelani Kamelamela </span><br/> <span class="individual-testimonial__position"> </span><br/> </div> </div> </div> <div class="col-12 col-sm-12 col-md-12 col-lg-2 quote-column text-center"> <img class="quote" loading="lazy" src="/themes/custom/bio_techne_global/images/testimonials-quote.svg"/> </div> </div> </div> <div class="row"> <div class="col-10 offset-1 col-md-10 offset-md-1 col-sm-10"> <div class="individual-testimonial__testimonial"> We've been using Bio-Techne's cell sorter to do single cell sorting for genomics/transcriptomics applications as well as for colony selection for tissue culture. We're overall very satisfied with our results and find the set up to be very easy, as well as standard maintenance. 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