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A mixture of these chemicals is known as '''embalming fluid''' and is used to preserve bodies of deceased persons for both funeral purposes and in medical research in anatomical laboratories. The period for which a body is embalmed is dependent on time, expertise of the embalmer and factors regarding duration of stay and purpose. Typically, embalming fluid contains a mixture of [[formaldehyde]], [[glutaraldehyde]], [[methanol]], and other solvents. The formaldehyde content generally ranges from 5&ndash;37% and the methanol content may range from 9&ndash;56%. In the United States alone, about 20 million liters (roughly 5.3 million gallons) of embalming fluid are used every year.<ref name="PERC">Sehee, Joe (2007). [http://perc.org/articles/green-burial-its-only-natural Green Burial: It's Only Natural], [[PERC Reports]], Winter 2007. Retrieved on 2013-11-06.</ref> == Method of operation == [[File:Atelier Nadar - Pedro II. (1825-1891), König von Brasilien, auf seinem Totenbett in Paris (Zeno Fotografie).jpg|thumb|Emperor [[Pedro II of Brazil]] lying in repose in 1891. He was embalmed on the day of his death. During the process, six liters (1.585 gallons) of [[hydrochloride]] of zinc and aluminum was injected into his [[common carotid artery]].{{sfn|Besouchet|1993|p=603}} In 1912, Brazilian educator and archivist Luís Gastão d'Escragnolle Dória went to the emperor's glass-topped coffin. At the occasion, he had noticed the well-preserved state of Pedro II's body.{{sfn|Rezzutti|2019|pp=498–499}}]] Embalming fluid acts to ''[[fixation (histology)|fix]]'' ([[denaturation (biochemistry)|denature]]) cellular proteins, meaning that they cannot act as a nutrient source for bacteria; embalming fluid also kills the bacteria themselves. Formaldehyde or glutaraldehyde fixes tissue or cells by irreversibly connecting a primary amine group in a protein molecule with a nearby nitrogen in a protein or DNA molecule through a -CH<sub>2</sub>- linkage called a [[Schiff base]]. The end result also creates the simulation, via color changes, of the appearance of blood flowing under the skin. Modern embalming is not done with a single fixative. Instead, various chemicals are used to create a mixture, called an arterial solution, which is uniquely generated for the needs of each case. For example, a body needing to be repatriated overseas needs a higher index (percentage of diluted preservative chemical) than one simply for [[viewing (funeral)|viewing]] (known in the United States and Canada as a [[Funeral#Visitation|funeral visitation]]) at a funeral home before [[cremation]] or burial. ==Process== [[File:Container containing embalming fluid.jpg|thumb|Tank containing embalming fluid]] {{main|Embalming}} Embalming fluid is injected into the arterial system of the deceased's abdomen and a [[trocar]] is inserted into the body cavity. The organs in the chest cavity and the abdomen are then punctured and drained of gas and fluid contents. Many other bodily fluids may also be displaced and removed from the body using the arterial system and in the case of cavity treatment aspirated from the body and replaced with a specialty fluid known as cavity fluid. ==Chemicals and additives== It is important to distinguish between an arterial chemical (or fluid), which is generally taken to be the product in its original composition, and an arterial solution, which is a diluted mixture of chemicals and made to order for each body. Non-preservative chemicals in an arterial solution are generally called "accessory chemicals" or co/pre-injectants, depending on their time of utilization. Potential ingredients in an arterial solution include: * Preservative (Arterial) Chemical. These are commonly a percentage (normally 18–37%) based mixture of [[formaldehyde]], [[glutaraldehyde]] or in some cases [[phenol]] which are then diluted to gain the final index of the arterial solution. Methanol is used to hold the formaldehyde in solution. [[Formalin]] refers specifically to 37% aqueous formaldehyde and is not commonly used in funeral embalming but rather in the preservation of anatomical specimens. * Water Conditioner. These are designed to balance the "hardness" of water (the presence of other trace chemicals that change the water's pH or neutrality) and to help reduce the deceased's acidity, a by-product of decomposition, as formaldehyde works best in an [[alkaline]] environment. Additionally, water conditioners may be used to help inactivate chemotherapy drugs and antibiotics, which may bind to and render ineffectual the preservative chemical. * Cell Conditioner. These chemicals act to prepare cells for absorption of arterial fluid and help break up clots in the bloodstream. * Dyes. Active dyes are used to restore the body's natural colouration and counterstain against conditions such as [[jaundice]] as well as to indicate distribution of arterial fluid. Inactive dyes are used by the manufacturer of the arterial fluid to give a pleasant color to the fluid in the bottle but do nothing for the appearance of the embalmed body. * [[Humectant]]s. These are added to dehydrated and emaciated bodies to help restore tissue to a more natural and hydrated appearance. * Anti-Edemic Chemicals. The opposite of humectants, these are designed to draw excessive fluid ([[edema]]) from a body. * Additional [[Disinfectant]]s. For certain cases, such as [[tissue gas]], [[speciality chemicals]] such as Omega Decomp Factor, Triton-28, STOP or Dispray (Topical) can be arterially injected to kill tissue gas. * Water. Most arterial solutions are a mix of some of the preceding chemicals with tepid water. Cases done without the addition of water are referred to as "waterless." Waterless embalming is more common in difficult cases or those requiring a very high degree of preservation, such as instances of an extended delay between death and final disposition. * Cavity Fluid. This is a generally a very high-index [[formaldehyde]] or [[glutaraldehyde]] solution injected undiluted directly via the trocar incision into the body cavities to treat the viscera. In cases of tissue gas, [[phenol]] based products are often used instead. ==History== Prior to the advent of the modern range of embalming chemicals a variety of alternative additives have been used by embalmers, including [[epsom salts]] for edema cases,{{Citation needed|date=May 2008}} but these are of limited effectiveness and can be chalked up as "embalmer tricks", as the validity of their use has never been demonstrated by professional embalmers or mortuary science programs. During the [[American Civil War]], the Union Army, wanting to transport dead soldiers from the battlefields back home for burial, consulted with Dr. Thomas Holmes, who developed a technique that involved draining a corpse's blood and embalming it with a fluid made with [[arsenic]] for preservation.<ref name="PERC"/> Embalming chemicals are generally produced by specialist manufacturers. The oldest embalming fluid company was founded as the Hill Fluid Company, in 1878, and was then incorporated by Dr. A.A. Bakker, as the Champion Company, in 1880, making The Champion Company 143 years old. Champion was still owned and operated by the Bakker Family until the death of Dr. Bakker's granddaughter, in the late 1970's. Champion still operates today and is still family owned by the Giankopulous Family. They continuously operate today. They are located in Springfield, OH. The Frigid Fluid Company was founded in 1892, followed by the Dodge Company in 1893, with other companies including Egyptian, now U.S. Chemical, as well as Kelco Supply Company (formerly L H Kellogg), Pierce Chemical Company (now owned by The Wilbert Company), Bondol Chemical Company, and Hydrol Chemical Company. There are many smaller and regional producers as well. Some funeral homes produce their own embalming fluids, although this practice has declined in recent decades as commercially available products have become of better quality and more readily available. Following the EU Biocides Legislation some pressure was brought to reduce the use of formaldehyde. [[International Agency for Research on Cancer|IARC]] Classes [[Formaldehyde]] as a [[:Category:IARC Group 1 carcinogens|Class 1 Carcinogen]]. There are alternatives to formaldehyde and phenol-based fluids, but these are technically not preservatives but rather sanitising agents and are not widely accepted. However, The Champion Company has always been aware of the safety of the embalmer and created and distributed lower exposure fluids with less HCHO and by the 1990s Champion was the first to create and distribute HCHO Free Fluids. Only The Champion and The Dodge Company sell those fluids. ==Environmental effects== Despite genuine concerns, formaldehyde is a naturally occurring substance, of which human beings produce approximately 1.5 oz a day as a normal part of a healthy metabolism. Formaldehyde also occurs naturally in many fruits, such as bananas, apples, and carrots, and does not bioaccumulate in either plants or animals. <ref>{{Cite web|url=https://formaldehyde.americanchemistry.com/Formaldehyde-Occurs-Naturally-and-Is-All-Around-Us.pdf|title = Formaldehyde| date=18 October 2023 }}</ref> Formaldehyde works to fixate the tissue of the deceased. This is the characteristic that also makes concentrated formaldehyde hazardous when not handled using appropriate [[personal protective equipment]]. The carbon atom in formaldehyde, CH<sub>2</sub>O, carries a slight positive charge due to the high [[electronegativity]] of the oxygen double bonded with the carbon. The electropositive carbon will react with a negatively charged molecule and other electron-rich species. As a result, the carbon in the formaldehyde molecule bonds with electron-rich nitrogen groups called [[amine]]s found in plant and animal tissue. This leads to formaldehyde [[cross-link]]ing, bonding proteins with other proteins and [[DNA]], rendering them dysfunctional or no longer useful.<ref name=":2">{{Cite web|title = Molecular Level Answers: The Real Reason Why Formaldehyde is Toxic – and how to avoid it|url = http://drholly.typepad.com/question_and_answer_forum/2011/06/th.html|website = drholly.typepad.com|access-date = 2015-11-28}}</ref> This is the reason for usage of formaldehyde as a preservative, as it thus prevents cellular decay and renders the tissue unsuitable for use as a nutrient source for bacteria. Formaldehyde is carcinogenic in humans and animals at excessive levels<ref name=":3">{{Cite web|title = Formaldehyde (HSG 57, 1991)|url = http://www.inchem.org/documents/hsg/hsg/hsg057.htm|website = www.inchem.org|access-date = 2015-11-28}}</ref><ref name=":4">{{Cite web|title = Formaldehyde CASRN 50-00-0 {{!}} IRIS {{!}} US EPA, ORD|url = http://cfpub.epa.gov/ncea/iris2/chemicalLanding.cfm?substance_nmbr=419|website = cfpub.epa.gov|access-date = 2015-11-28|first = US EPA, ORD, Integrated Risk Information System|last = Division}}</ref> because the cross-linking can cause DNA to keep cells from halting the replication process. This unwarranted replication of cells can lead to [[cancer]].<ref name=":2" /> Unicellular organisms found in the soil and groundwater are also quite sensitive to cross-linking, experiencing damage at a concentration of 0.3&nbsp;mg to 22&nbsp;mg per liter.<ref name=":3" /> Formaldehyde also affects aquatic invertebrates, with [[crustacean]]s being the most sensitive type. The range of concentration damaging them is 0.4&nbsp;mg to 20&nbsp;mg per liter.<ref name=":3" /> Formaldehyde released from the cremation of embalmed bodies enters the atmosphere and can remain suspended for up to 250 hours.<ref name=":3" /> It is readily soluble<ref name=":4" /> in water so it will bond with moisture in the atmosphere and rain down onto plants, animals, and water supplies below. As a result, formaldehyde content in precipitation can range from 110 μg to 1380 μg per liter.<ref name=":3" /> These concerns notwithstanding, according to the [[American Chemistry Council]], formaldehyde, as a ubiquitous chemical produced by living beings, is eminently biodegradable by both sunlight in air and bacteria in soil and water. <ref>{{Cite web |title=Formaldehyde Is Biodegradable, Quickly Broken Down in the Air By Sunlight or By Bacteria in Soil or Water |url=https://www.americanchemistry.com/Media/PressReleasesTranscripts/ACC-news-releases/Formaldehyde-Is-Biodegradable.html |url-status=dead |archive-url=https://web.archive.org/web/20210210194558/https://www.americanchemistry.com/Media/PressReleasesTranscripts/ACC-news-releases/Formaldehyde-Is-Biodegradable.html |archive-date=2021-02-10}}</ref> The growth of the environment movement has caused some people to consider [[Natural burial|green burials]] where there are either no aldehyde-based chemicals used in the embalming process, or there is no embalming process at all.<ref>{{Cite news|title = Dying to Be Green|last = Shea|first = Neil|date = July 2008|work = magazine|via = National Geographic}}</ref> Embalming fluid meeting specific criteria for such burials is commercially available, and although it is not as effective as aldehyde-based solutions, is approved by the Green Burial Association of America. Only the Champion Company has created and distributed their 4th generation of fluids called "Enigma", created in the early 2000's. All of Champion's enigma products have been approved by the green Burial Council. ==See also== * [[Glass House (British Columbia)]] – a building in British Columbia constructed with empty embalming fluid bottles ==References== {{reflist}} ==Further reading== {{refbegin}} * Abrams, J.L. ''Embalming''. 2008. * Frederick, L.G.; Strub, Clarence G. [1959] (1989). ''The Principles and Practice of Embalming'', 5th ed., Dallas, TX: Professional Training Schools Inc & Robertine Frederick. {{OCLC|20723376}}. * Mayer, Robert G. (2000). ''Embalming: History, Theory and Practice,'' 3rd ed., McGraw-Hill/Appleton & Lange. {{ISBN|978-0838521878}}. * {{cite book |author-last=Schmitz-Esser |author-first=Romedio |year=2013 |chapter=Embalming and Dissecting the Corpse between East and West: From ar-Razi to Henry de Mondeville |chapter-url=https://books.google.com/books?id=snPnBQAAQBAJ&pg=PA611 |editor-last=Classen |editor-first=Albrecht |title=East Meets West in the Middle Ages and Early Modern Times: Transcultural Experiences in the Premodern World |location=[[Berlin]] and [[Boston]] |publisher=[[De Gruyter]] |series=Fundamentals of Medieval and Early Modern Culture |volume=14 |pages=611–624 |doi=10.1515/9783110321517.611 |isbn=978-3110328783 |issn=1864-3396 }} * {{cite book| last1 = Besouchet| first1 = Lídia| year = 1993| language = Portuguese| title = Pedro&nbsp;II e o Século&nbsp;XIX| edition = 2nd| publisher = Nova Fronteira| location = Rio de Janeiro | isbn = 978-85-209-0494-7}} * {{cite book| last1 = Rezzutti| first1 = Paulo| year = 2019| language = pt| title = D. Pedro II: a história não contada: O último imperador do Novo Mundo revelado por cartas e documentos inéditos| publisher = Leya; 2019| location = | isbn = 978-85-7734-677-6}} {{refend}} == External links == * [http://thechampioncompany.com/ Official Champion Company Website] * [http://www.kelcosupply.com/ Official Kelco Supply Company Website] * [http://www.piercedirect.com/ Official Pierce Chemical Company Website] * [http://www.frigidfluid.com/ Official Frigid Fluid Company Website] * [http://www.trinityfluids.com/ Official Trinity Fluids, LLC website] * [http://www.dodgeco.com/ Official Dodge Company Website] * [https://web.archive.org/web/20120206141309/http://aardbalm.com/ Official Aardbalm Company Website] * [https://web.archive.org/web/20070313172757/http://video.google.co.uk/videoplay?docid=2387318321935223597 CGI Embalming using Aardbalm] [[Category:Chemical substances by use]] [[Category:Death customs]] [[Category:Anatomical preservation]] </textarea><div 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