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Tilgjengelig på 24 språk" > <label id="p-lang-btn-label" for="p-lang-btn-checkbox" class="vector-dropdown-label cdx-button cdx-button--fake-button cdx-button--fake-button--enabled cdx-button--weight-quiet cdx-button--action-progressive mw-portlet-lang-heading-24" aria-hidden="true" ><span class="vector-icon mw-ui-icon-language-progressive mw-ui-icon-wikimedia-language-progressive"></span> <span class="vector-dropdown-label-text">24 språk</span> </label> <div class="vector-dropdown-content"> <div class="vector-menu-content"> <ul class="vector-menu-content-list"> <li class="interlanguage-link interwiki-nn mw-list-item"><a href="https://nn.wikipedia.org/wiki/UV/VIS-spektroskopi" title="UV/VIS-spektroskopi – norsk nynorsk" lang="nn" hreflang="nn" data-title="UV/VIS-spektroskopi" data-language-autonym="Norsk nynorsk" data-language-local-name="norsk nynorsk" class="interlanguage-link-target"><span>Norsk nynorsk</span></a></li><li class="interlanguage-link interwiki-ar mw-list-item"><a href="https://ar.wikipedia.org/wiki/%D9%85%D8%B7%D9%8A%D8%A7%D9%81%D9%8A%D8%A9_%D8%A7%D9%84%D8%A3%D8%B4%D8%B9%D8%A9_%D8%A7%D9%84%D9%85%D8%B1%D8%A6%D9%8A%D8%A9_%D9%88%D9%81%D9%88%D9%82_%D8%A7%D9%84%D8%A8%D9%86%D9%81%D8%B3%D8%AC%D9%8A%D8%A9" title="مطيافية الأشعة المرئية وفوق البنفسجية – arabisk" lang="ar" hreflang="ar" data-title="مطيافية الأشعة المرئية وفوق البنفسجية" data-language-autonym="العربية" data-language-local-name="arabisk" class="interlanguage-link-target"><span>العربية</span></a></li><li class="interlanguage-link interwiki-bg mw-list-item"><a href="https://bg.wikipedia.org/wiki/%D0%9E%D0%BF%D1%82%D0%B8%D1%87%D0%BD%D0%B0_%D1%81%D0%BF%D0%B5%D0%BA%D1%82%D1%80%D0%BE%D1%81%D0%BA%D0%BE%D0%BF%D0%B8%D1%8F" title="Оптична спектроскопия – bulgarsk" lang="bg" hreflang="bg" data-title="Оптична спектроскопия" data-language-autonym="Български" data-language-local-name="bulgarsk" class="interlanguage-link-target"><span>Български</span></a></li><li class="interlanguage-link interwiki-bs mw-list-item"><a href="https://bs.wikipedia.org/wiki/Ultravioletna-vidljiva_spektroskopija" title="Ultravioletna-vidljiva spektroskopija – bosnisk" lang="bs" hreflang="bs" data-title="Ultravioletna-vidljiva spektroskopija" data-language-autonym="Bosanski" data-language-local-name="bosnisk" class="interlanguage-link-target"><span>Bosanski</span></a></li><li class="interlanguage-link interwiki-ca mw-list-item"><a href="https://ca.wikipedia.org/wiki/Espectrosc%C3%B2pia_ultraviolada-visible" title="Espectroscòpia ultraviolada-visible – katalansk" lang="ca" hreflang="ca" data-title="Espectroscòpia ultraviolada-visible" data-language-autonym="Català" data-language-local-name="katalansk" class="interlanguage-link-target"><span>Català</span></a></li><li class="interlanguage-link interwiki-cs mw-list-item"><a href="https://cs.wikipedia.org/wiki/Ultrafialovo-viditeln%C3%A1_spektroskopie" title="Ultrafialovo-viditelná spektroskopie – tsjekkisk" lang="cs" hreflang="cs" data-title="Ultrafialovo-viditelná spektroskopie" data-language-autonym="Čeština" data-language-local-name="tsjekkisk" class="interlanguage-link-target"><span>Čeština</span></a></li><li class="interlanguage-link interwiki-de mw-list-item"><a href="https://de.wikipedia.org/wiki/UV/VIS-Spektroskopie" title="UV/VIS-Spektroskopie – tysk" lang="de" hreflang="de" data-title="UV/VIS-Spektroskopie" data-language-autonym="Deutsch" data-language-local-name="tysk" class="interlanguage-link-target"><span>Deutsch</span></a></li><li class="interlanguage-link interwiki-et mw-list-item"><a href="https://et.wikipedia.org/wiki/UV/Vis-spektroskoopia" title="UV/Vis-spektroskoopia – estisk" lang="et" hreflang="et" data-title="UV/Vis-spektroskoopia" data-language-autonym="Eesti" data-language-local-name="estisk" class="interlanguage-link-target"><span>Eesti</span></a></li><li class="interlanguage-link interwiki-en mw-list-item"><a href="https://en.wikipedia.org/wiki/Ultraviolet%E2%80%93visible_spectroscopy" title="Ultraviolet–visible spectroscopy – engelsk" lang="en" hreflang="en" data-title="Ultraviolet–visible spectroscopy" data-language-autonym="English" data-language-local-name="engelsk" class="interlanguage-link-target"><span>English</span></a></li><li class="interlanguage-link interwiki-es mw-list-item"><a href="https://es.wikipedia.org/wiki/Espectroscopia_ultravioleta-visible" title="Espectroscopia ultravioleta-visible – spansk" lang="es" hreflang="es" data-title="Espectroscopia ultravioleta-visible" data-language-autonym="Español" data-language-local-name="spansk" class="interlanguage-link-target"><span>Español</span></a></li><li class="interlanguage-link interwiki-fa mw-list-item"><a href="https://fa.wikipedia.org/wiki/%D8%B7%DB%8C%D9%81%E2%80%8C%D8%B4%D9%86%D8%A7%D8%B3%DB%8C_%D9%85%D8%B1%D8%A6%DB%8C%E2%80%93%D9%81%D8%B1%D8%A7%D8%A8%D9%86%D9%81%D8%B4" title="طیف‌شناسی مرئی–فرابنفش – persisk" lang="fa" hreflang="fa" data-title="طیف‌شناسی مرئی–فرابنفش" data-language-autonym="فارسی" data-language-local-name="persisk" class="interlanguage-link-target"><span>فارسی</span></a></li><li class="interlanguage-link interwiki-fr mw-list-item"><a href="https://fr.wikipedia.org/wiki/Spectroscopie_ultraviolet-visible" title="Spectroscopie ultraviolet-visible – fransk" lang="fr" hreflang="fr" data-title="Spectroscopie ultraviolet-visible" data-language-autonym="Français" data-language-local-name="fransk" class="interlanguage-link-target"><span>Français</span></a></li><li class="interlanguage-link interwiki-ko mw-list-item"><a href="https://ko.wikipedia.org/wiki/%EC%9E%90%EC%99%B8%EC%84%A0_%EA%B0%80%EC%8B%9C%EA%B4%91%EC%84%A0_%EB%B6%84%EA%B4%91%EB%B2%95" title="자외선 가시광선 분광법 – koreansk" lang="ko" hreflang="ko" data-title="자외선 가시광선 분광법" data-language-autonym="한국어" data-language-local-name="koreansk" class="interlanguage-link-target"><span>한국어</span></a></li><li class="interlanguage-link interwiki-it mw-list-item"><a href="https://it.wikipedia.org/wiki/Spettroscopia_ultravioletta/visibile" title="Spettroscopia ultravioletta/visibile – italiensk" lang="it" hreflang="it" data-title="Spettroscopia ultravioletta/visibile" data-language-autonym="Italiano" data-language-local-name="italiensk" class="interlanguage-link-target"><span>Italiano</span></a></li><li class="interlanguage-link interwiki-nl mw-list-item"><a href="https://nl.wikipedia.org/wiki/UV/VIS-spectroscopie" title="UV/VIS-spectroscopie – nederlandsk" lang="nl" hreflang="nl" data-title="UV/VIS-spectroscopie" data-language-autonym="Nederlands" data-language-local-name="nederlandsk" class="interlanguage-link-target"><span>Nederlands</span></a></li><li class="interlanguage-link interwiki-ja mw-list-item"><a href="https://ja.wikipedia.org/wiki/%E7%B4%AB%E5%A4%96%E5%8F%AF%E8%A6%96%E8%BF%91%E8%B5%A4%E5%A4%96%E5%88%86%E5%85%89%E6%B3%95" title="紫外可視近赤外分光法 – japansk" lang="ja" hreflang="ja" data-title="紫外可視近赤外分光法" data-language-autonym="日本語" data-language-local-name="japansk" class="interlanguage-link-target"><span>日本語</span></a></li><li class="interlanguage-link interwiki-pl mw-list-item"><a href="https://pl.wikipedia.org/wiki/Spektroskopia_UV-VIS" title="Spektroskopia UV-VIS – polsk" lang="pl" hreflang="pl" data-title="Spektroskopia UV-VIS" data-language-autonym="Polski" data-language-local-name="polsk" class="interlanguage-link-target"><span>Polski</span></a></li><li class="interlanguage-link interwiki-pt mw-list-item"><a href="https://pt.wikipedia.org/wiki/Espectroscopia_UV/vis%C3%ADvel" title="Espectroscopia UV/visível – portugisisk" lang="pt" hreflang="pt" data-title="Espectroscopia UV/visível" data-language-autonym="Português" data-language-local-name="portugisisk" class="interlanguage-link-target"><span>Português</span></a></li><li class="interlanguage-link interwiki-ru mw-list-item"><a href="https://ru.wikipedia.org/wiki/%D0%9E%D0%BF%D1%82%D0%B8%D1%87%D0%B5%D1%81%D0%BA%D0%B0%D1%8F_%D1%81%D0%BF%D0%B5%D0%BA%D1%82%D1%80%D0%BE%D1%81%D0%BA%D0%BE%D0%BF%D0%B8%D1%8F" title="Оптическая спектроскопия – russisk" lang="ru" hreflang="ru" data-title="Оптическая спектроскопия" data-language-autonym="Русский" data-language-local-name="russisk" class="interlanguage-link-target"><span>Русский</span></a></li><li class="interlanguage-link interwiki-simple mw-list-item"><a href="https://simple.wikipedia.org/wiki/Ultraviolet%E2%80%93visible_absorption" title="Ultraviolet–visible absorption – enkel engelsk" lang="en-simple" hreflang="en-simple" data-title="Ultraviolet–visible absorption" data-language-autonym="Simple English" data-language-local-name="enkel engelsk" class="interlanguage-link-target"><span>Simple English</span></a></li><li class="interlanguage-link interwiki-uk mw-list-item"><a href="https://uk.wikipedia.org/wiki/%D0%A3%D0%BB%D1%8C%D1%82%D1%80%D0%B0%D1%84%D1%96%D0%BE%D0%BB%D0%B5%D1%82%D0%BE%D0%B2%D0%B0_%D1%81%D0%BF%D0%B5%D0%BA%D1%82%D1%80%D0%BE%D1%81%D0%BA%D0%BE%D0%BF%D1%96%D1%8F" title="Ультрафіолетова спектроскопія – ukrainsk" lang="uk" hreflang="uk" data-title="Ультрафіолетова спектроскопія" data-language-autonym="Українська" data-language-local-name="ukrainsk" class="interlanguage-link-target"><span>Українська</span></a></li><li class="interlanguage-link interwiki-vi mw-list-item"><a href="https://vi.wikipedia.org/wiki/M%C3%A1y_quang_ph%E1%BB%95_c%E1%BB%B1c_t%C3%ADm_-_Kh%E1%BA%A3_ki%E1%BA%BFn" title="Máy quang phổ cực tím - Khả kiến – vietnamesisk" lang="vi" hreflang="vi" data-title="Máy quang phổ cực tím - Khả kiến" data-language-autonym="Tiếng Việt" data-language-local-name="vietnamesisk" class="interlanguage-link-target"><span>Tiếng Việt</span></a></li><li class="interlanguage-link interwiki-zh-yue mw-list-item"><a href="https://zh-yue.wikipedia.org/wiki/%E7%B4%AB%E5%A4%96-%E5%8F%AF%E8%A6%8B%E5%88%86%E5%85%89%E5%85%89%E5%BA%A6%E6%B3%95" title="紫外-可見分光光度法 – kantonesisk" lang="yue" hreflang="yue" data-title="紫外-可見分光光度法" data-language-autonym="粵語" data-language-local-name="kantonesisk" class="interlanguage-link-target"><span>粵語</span></a></li><li class="interlanguage-link interwiki-zh mw-list-item"><a href="https://zh.wikipedia.org/wiki/%E7%B4%AB%E5%A4%96-%E5%8F%AF%E8%A7%81%E5%88%86%E5%85%89%E5%85%89%E5%BA%A6%E6%B3%95" title="紫外-可见分光光度法 – kinesisk" lang="zh" hreflang="zh" data-title="紫外-可见分光光度法" data-language-autonym="中文" data-language-local-name="kinesisk" class="interlanguage-link-target"><span>中文</span></a></li> </ul> <div class="after-portlet after-portlet-lang"><span class="wb-langlinks-edit wb-langlinks-link"><a href="https://www.wikidata.org/wiki/Special:EntityPage/Q898426#sitelinks-wikipedia" title="Rediger lenker til artikkelen på andre språk" class="wbc-editpage">Rediger 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class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/e/ed/DU640_spectrophotometer.jpg/250px-DU640_spectrophotometer.jpg" decoding="async" width="250" height="144" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/e/ed/DU640_spectrophotometer.jpg/375px-DU640_spectrophotometer.jpg 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/e/ed/DU640_spectrophotometer.jpg/500px-DU640_spectrophotometer.jpg 2x" data-file-width="3984" data-file-height="2298" /></a><figcaption>Beckman DU640 UV/Vis spektrofotometer</figcaption></figure> <p><b>Ultrafiolett–synlig spektroskopi</b> (fra engelsk <i>Ultraviolet–visible spectroscopy</i>, forkortet <i>UV/Vis</i>) refererer til <a href="/wiki/Absorpsjonsspekter" title="Absorpsjonsspekter">absorpsjonsspekter</a> i det ultrafiolette/synlige område i spektret for stårling. Dette betyr at det bruker lys i det synlige og nærligende områdene området. Absorpsjonen eller refleksjonen i det synlige området vil gi direkte innvirkning av fargen til kjemikaliet som er målt. I dette området av det <a href="/wiki/Elektromagnetisk_spekter" title="Elektromagnetisk spekter">elektromagnetiske spekteret</a>, atomer eller molekyler får elektromagnetiske sprang. Absorpsjonsspektrometri er komplementær til <a href="/w/index.php?title=Fluorescensspektrometri&action=edit&redlink=1" class="new" title="Fluorescensspektrometri (ikke skrevet ennå)">fluorescensspektrometri</a>, i at <a href="/wiki/Fluorescens" title="Fluorescens">fluorescens</a> omhandler overganger fra den eksiterete tilstanden til grunntilstanden, absorpsjon måler overganger fra grunntilstanden til den eksiterte tilstanden<sup id="cite_ref-1" class="reference"><a href="#cite_note-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup>. </p> <div class="mw-heading mw-heading2"><h2 id="Prinsippet_av_uv/vis_absorpsjon"><span id="Prinsippet_av_uv.2Fvis_absorpsjon"></span>Prinsippet av uv/vis absorpsjon</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Ultrafiolett%E2%80%93synlig_spektroskopi&veaction=edit&section=1" title="Rediger avsnitt: Prinsippet av uv/vis absorpsjon" class="mw-editsection-visualeditor"><span>rediger</span></a><span class="mw-editsection-divider"> | </span><a href="/w/index.php?title=Ultrafiolett%E2%80%93synlig_spektroskopi&action=edit&section=1" title="Rediger kildekoden til seksjonen Prinsippet av uv/vis absorpsjon"><span>rediger kilde</span></a><span class="mw-editsection-bracket">]</span></span></div> <figure class="mw-halign-right" typeof="mw:File/Thumb"><a href="/wiki/Fil:Bis(triphenylphosphine)_nickel_(II)_chloride_UV-vis.JPG" class="mw-file-description"><img src="//upload.wikimedia.org/wikipedia/commons/thumb/9/9e/Bis%28triphenylphosphine%29_nickel_%28II%29_chloride_UV-vis.JPG/300px-Bis%28triphenylphosphine%29_nickel_%28II%29_chloride_UV-vis.JPG" decoding="async" width="300" height="234" class="mw-file-element" srcset="//upload.wikimedia.org/wikipedia/commons/thumb/9/9e/Bis%28triphenylphosphine%29_nickel_%28II%29_chloride_UV-vis.JPG/450px-Bis%28triphenylphosphine%29_nickel_%28II%29_chloride_UV-vis.JPG 1.5x, //upload.wikimedia.org/wikipedia/commons/thumb/9/9e/Bis%28triphenylphosphine%29_nickel_%28II%29_chloride_UV-vis.JPG/600px-Bis%28triphenylphosphine%29_nickel_%28II%29_chloride_UV-vis.JPG 2x" data-file-width="752" data-file-height="587" /></a><figcaption>Et eksempelt på UV/Vis avlesning</figcaption></figure> <p>Molekyler som har π-elektroner eller ikke-bindende elektroner (n-elektroner) kan absorbere energi i form av ultrafiolett eller synlig lys for å eksitere disse elektronene til en høyere anti-bindende molekylorbital<sup id="cite_ref-2" class="reference"><a href="#cite_note-2"><span class="cite-bracket">[</span>2<span class="cite-bracket">]</span></a></sup>. Jo lettere elektronene er eksitert (dvs. lavere energigap mellom HOMO og LUMO) vil bølgelengden til lyse som blir absorbert bli større. Det er fire mulige typer sprang (π–π*, n–π*, σ–σ*, og n–σ*), og de kan bli ordnet slik: σ–σ* > n–σ* > π–π* > n–π*. </p> <div class="mw-heading mw-heading2"><h2 id="Referanser">Referanser</h2><span class="mw-editsection"><span class="mw-editsection-bracket">[</span><a href="/w/index.php?title=Ultrafiolett%E2%80%93synlig_spektroskopi&veaction=edit&section=2" title="Rediger avsnitt: Referanser" class="mw-editsection-visualeditor"><span>rediger</span></a><span class="mw-editsection-divider"> | </span><a href="/w/index.php?title=Ultrafiolett%E2%80%93synlig_spektroskopi&action=edit&section=2" title="Rediger kildekoden til seksjonen Referanser"><span>rediger kilde</span></a><span class="mw-editsection-bracket">]</span></span></div> <div class="mw-references-wrap"><ol class="references"> <li id="cite_note-1"><b><a href="#cite_ref-1">^</a></b> <span class="reference-text"><cite class="citation book">A.,, Skoog, Douglas. <a rel="nofollow" class="external text" href="https://www.worldcat.org/oclc/77224390"><i>Principles of instrumental analysis</i></a> (Sixth edition utg.). Belmont, CA. <a href="/wiki/ISBN" title="ISBN">ISBN</a> <a href="/wiki/Spesial:Bokkilder/9780495012016" title="Spesial:Bokkilder/9780495012016">9780495012016</a>. <a href="/wiki/OCLC" class="mw-redirect" title="OCLC">OCLC</a> <a rel="nofollow" class="external text" href="//www.worldcat.org/oclc/77224390">77224390</a>.</cite><span title="ctx_ver=Z39.88-2004&rfr_id=info%3Asid%2Fno.wikipedia.org%3AUltrafiolett%E2%80%93synlig+spektroskopi&rft.aufirst=Skoog%2C+Douglas&rft.aulast=A.%2C&rft.btitle=Principles+of+instrumental+analysis&rft.edition=Sixth+edition&rft.genre=book&rft.isbn=9780495012016&rft.place=Belmont%2C+CA&rft_id=https%3A%2F%2Fwww.worldcat.org%2Foclc%2F77224390&rft_id=info%3Aoclcnum%2F77224390&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Abook" class="Z3988"><span style="display:none;"> </span></span><span class="citation-comment" style="display:none; color:#33aa33; margin-left:0.3em">CS1-vedlikehold: Ekstra tekst (<a href="/wiki/Kategori:CS1-vedlikehold:_Ekstra_tekst" title="Kategori:CS1-vedlikehold: Ekstra tekst">link</a>)</span></span> </li> <li id="cite_note-2"><b><a href="#cite_ref-2">^</a></b> <span class="reference-text"><cite class="citation news"><a rel="nofollow" class="external text" href="http://pharmaxchange.info/press/2011/12/ultraviolet-visible-uv-vis-spectroscopy-principle/">«Ultraviolet-Visible (UV-Vis) Spectroscopy | Analytical Chemistry | PharmaXChange.info»</a>. <i>PharmaXChange.info</i> (på engelsk). 13. desember 2011<span class="reference-accessdate">. Besøkt 17. oktober 2018</span>.</cite><span title="ctx_ver=Z39.88-2004&rfr_id=info%3Asid%2Fno.wikipedia.org%3AUltrafiolett%E2%80%93synlig+spektroskopi&rft.atitle=Ultraviolet-Visible+%28UV-Vis%29+Spectroscopy++%7C+Analytical+Chemistry+%7C+PharmaXChange.info&rft.date=2011-12-13&rft.genre=article&rft.jtitle=PharmaXChange.info&rft_id=http%3A%2F%2Fpharmaxchange.info%2Fpress%2F2011%2F12%2Fultraviolet-visible-uv-vis-spectroscopy-principle%2F&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal" class="Z3988"><span style="display:none;"> </span></span></span> </li> </ol></div> <div class="navbox-styles"><style data-mw-deduplicate="TemplateStyles:r23230704">.mw-parser-output .hlist dl,.mw-parser-output .hlist ol,.mw-parser-output .hlist ul{margin:0;padding:0}.mw-parser-output .hlist dd,.mw-parser-output .hlist dt,.mw-parser-output .hlist li{margin:0;display:inline}.mw-parser-output .hlist.inline,.mw-parser-output .hlist.inline dl,.mw-parser-output .hlist.inline ol,.mw-parser-output .hlist.inline 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