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vesicles</title> <meta name="description" content="Search results for: phospholipid vesicles"> <meta name="keywords" content="phospholipid vesicles"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none 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action="https://publications.waset.org/abstracts/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="phospholipid vesicles"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 86</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: phospholipid vesicles</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">86</span> Localisation of Fluorescently Labelled Drug-Free Phospholipid Vesicles to the Cartilage Surface of Rat Synovial Joints</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sam%20Yurdakul">Sam Yurdakul</a>, <a href="https://publications.waset.org/abstracts/search?q=Nick%20Baverstock"> Nick Baverstock</a>, <a href="https://publications.waset.org/abstracts/search?q=Jim%20Mills"> Jim Mills</a> </p> <p class="card-text"><strong>Abstract:</strong></p> TDT 064 (FLEXISEQ®) is a drug-free gel used to treat osteoarthritis (OA)-associated pain and joint stiffness. It contains ultra-deformable phospholipid Sequessome™ vesicles, which can pass through the skin barrier intact. In six randomized OA studies, topical TDT 064 was well tolerated and improved joint pain, physical function and stiffness. In the largest study, these TDT 064-mediated effects were statistically significantly greater than oral placebo and equivalent to celecoxib. To understand the therapeutic effects of TDT 064, we investigated the localisation of the drug-free vesicles within rat synovial joints. TDT 064 containing DiO-labelled Sequessome™ vesicles was applied to the knees of four 6-week-old CD® hairless rats (10 mg/kg/ joint), 2–3 times/day, for 3 days (representing the recommended clinical dose). Eighteen hours later, the animals and one untreated control were sacrificed, and the knee joints isolated, flash frozen and embedded in Acrytol Mounting Media™. Approximately 15 sections (10 µm) from each joint were analysed by fluorescence microscopy. To investigate whether the localisation of DiO fluorescence was associated with intact vesicles, an anti-PEG monoclonal antibody (mAb) was used to detect Tween, a constituent of Sequessome™ vesicles. Sections were visualized at 484 nm (DiO) and 647 nm (anti-PEG mAb) and analysed using inForm 1.4 (Perkin Elmer, Inc.). Significant fluorescence was observed at 484 nm in sections from TDT 064-treated animals. No non-specific fluorescence was observed in control sections. Fluorescence was detected as discrete vesicles on the cartilage surfaces, inside the cartilaginous matrix and within the synovial space. The number of DiO-labelled vesicles in multiple fields of view was consistent and >100 in sections from four different treated knees. DiO and anti-PEG mAb co-localised within the collagenous tissues in four different joint sections. Under higher magnification (40x), vesicles were seen in the intercellular spaces of the synovial joint tissue, but no fluorescence was seen inside cells. These data suggest that the phospholipid vesicles in TDT 064 localize at the surface of the joint cartilage; these vesicles may therefore be supplementing the phospholipid deficiency reported in OA and acting as a biolubricant within the synovial joint. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=joint%20pain" title="joint pain">joint pain</a>, <a href="https://publications.waset.org/abstracts/search?q=osteoarthritis" title=" osteoarthritis"> osteoarthritis</a>, <a href="https://publications.waset.org/abstracts/search?q=phospholipid%20vesicles" title=" phospholipid vesicles"> phospholipid vesicles</a>, <a href="https://publications.waset.org/abstracts/search?q=TDT%20064" title=" TDT 064"> TDT 064</a> </p> <a href="https://publications.waset.org/abstracts/22741/localisation-of-fluorescently-labelled-drug-free-phospholipid-vesicles-to-the-cartilage-surface-of-rat-synovial-joints" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22741.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">443</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">85</span> Synthesis of Liposomal Vesicles by a Novel Supercritical Fluid Process</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Wen-Chyan%20Tsai">Wen-Chyan Tsai</a>, <a href="https://publications.waset.org/abstracts/search?q=Syed%20S.%20H.%20Rizvi"> Syed S. H. Rizvi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Organic solvent residues are always associated with liposomes produced by the traditional techniques like the thin film hydration and reverse phase evaporation methods, which limit the applications of these vesicles in the pharmaceutical, food and cosmetic industries. Our objective was to develop a novel and benign process of liposomal microencapsulation by using supercritical carbon dioxide (SC-CO2) as the sole phospholipid-dissolving medium and a green substitute for organic solvents. This process consists of supercritical fluid extraction followed by rapid expansion via a nozzle and automatic cargo suction. Lecithin and cholesterol mixed in 10:1 mass ratio were dissolved in SC-CO2 at 20 ± 0.5 MPa and 60 oC. After at least two hours of equilibrium, the lecithin/cholesterol-laden SC-CO2 was passed through a 1000-micron nozzle and immediately mixed with the cargo solution to form liposomes. Liposomal micro-encapsulation was conducted at three pressures (8.27, 12.41, 16.55 MPa), three temperatures (75, 83 and 90 oC) and two flow rates (0.25 ml/sec and 0.5 ml/sec). Liposome size, zeta potential and encapsulation efficiency were characterized as functions of the operating parameters. The average liposomal size varied from 400-500 nm to 1000-1200 nm when the pressure was increased from 8.27 to 16.55 MPa. At 12.41 MPa, 90 oC and 0.25 ml per second of 0.2 M glucose cargo loading rate, the highest encapsulation efficiency of 31.65 % was achieved. Under a confocal laser scanning microscope, large unilamellar vesicles and multivesicular vesicles were observed to make up a majority of the liposomal emulsion. This new approach is a rapid and continuous process for bulk production of liposomes using a green solvent. Based on the results to date, it is feasible to apply this technique to encapsulate hydrophilic compounds inside the aqueous core as well as lipophilic compounds in the phospholipid bilayers of the liposomes for controlled release, solubility improvement and targeted therapy of bioactive compounds. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=liposome" title="liposome">liposome</a>, <a href="https://publications.waset.org/abstracts/search?q=micro%20encapsulation" title=" micro encapsulation"> micro encapsulation</a>, <a href="https://publications.waset.org/abstracts/search?q=supercritical%20carbon%20dioxide" title=" supercritical carbon dioxide"> supercritical carbon dioxide</a>, <a href="https://publications.waset.org/abstracts/search?q=non-toxic%20process" title=" non-toxic process "> non-toxic process </a> </p> <a href="https://publications.waset.org/abstracts/22475/synthesis-of-liposomal-vesicles-by-a-novel-supercritical-fluid-process" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22475.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">431</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">84</span> Sterols Regulate the Activity of Phospholipid Scramblase by Interacting through Putative Cholesterol Binding Motif</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhasin%20Koyiloth">Muhasin Koyiloth</a>, <a href="https://publications.waset.org/abstracts/search?q=Sathyanarayana%20N.%20Gummadi"> Sathyanarayana N. Gummadi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Biological membranes are ordered association of lipids, proteins, and carbohydrates. Lipids except sterols possess asymmetric distribution across the bilayer. Eukaryotic membranes possess a group of lipid translocators called scramblases that disrupt phospholipid asymmetry. Their action is implicated in cell activation during wound healing and phagocytic clearance of apoptotic cells. Cholesterol is one of the major membrane lipids distributed evenly on both the leaflet and can directly influence the membrane fluidity through the ordering effect. The fluidity has an impact on the activity of several membrane proteins. The palmitoylated phospholipid scramblases localized to the lipid raft which is characterized by a higher number of sterols. Here we propose that cholesterol can interact with scramblases through putative CRAC motif and can modulate their activity. To prove this, we reconstituted phospholipid scramblase 1 of C. elegans (SCRM-1) in proteoliposomes containing different amounts of cholesterol (Liquid ordered/Lo). We noted that the presence of cholesterol reduced the scramblase activity of wild-type SCRM-1. The interaction between SCRM-1 and cholesterol was confirmed by fluorescence spectroscopy using NBD-Chol. Also, we observed loss of such interaction when one of I273 in the CRAC motif mutated to Asp. Interestingly, the point mutant has partially retained scramblase activity in Lo vesicles. The current study elucidated the important interaction between cholesterol and SCRM-1 to fine-tune its activity in artificial membranes. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=artificial%20membranes" title="artificial membranes">artificial membranes</a>, <a href="https://publications.waset.org/abstracts/search?q=CRAC%20motif" title=" CRAC motif"> CRAC motif</a>, <a href="https://publications.waset.org/abstracts/search?q=plasma%20membrane" title=" plasma membrane"> plasma membrane</a>, <a href="https://publications.waset.org/abstracts/search?q=PL%20scramblase" title=" PL scramblase"> PL scramblase</a> </p> <a href="https://publications.waset.org/abstracts/137353/sterols-regulate-the-activity-of-phospholipid-scramblase-by-interacting-through-putative-cholesterol-binding-motif" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/137353.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">175</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">83</span> Ultra-deformable Drug-free Sequessome™ Vesicles (TDT 064) for the Treatment of Joint Pain Following Exercise: A Case Report and Clinical Data</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Joe%20Collins">Joe Collins</a>, <a href="https://publications.waset.org/abstracts/search?q=Matthias%20Rother"> Matthias Rother</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Oral non-steroidal anti-inflammatory drugs (NSAIDs) are widely used for the relief of joint pain during and post-exercise. However, oral NSAIDs increase the risk of systemic side effects, even in healthy individuals, and retard recovery from muscle soreness. TDT 064 (Flexiseq®), a topical formulation containing ultra-deformable drug-free Sequessome™ vesicles, has demonstrated equivalent efficacy to oral celecoxib in reducing osteoarthritis-associated joint pain and stiffness. TDT 064 does not cause NSAID-related adverse effects. We describe clinical study data and a case report on the effectiveness of TDT 064 in reducing joint pain after exercise. Methods: Participants with a pain score ≥3 (10-point scale) 12–16 hours post-exercise were randomized to receive TDT 064 plus oral placebo, TDT 064 plus oral ketoprofen, or ketoprofen in ultra-deformable phospholipid vesicles plus oral placebo. Results: In the 168 study participants, pain scores were significantly higher with oral ketoprofen plus TDT 064 than with TDT 064 plus placebo in the 7 days post-exercise (P = 0.0240) and recovery from muscle soreness was significantly longer (P = 0.0262). There was a low incidence of adverse events. These data are supported by clinical experience. A 24-year-old male professional rugby player suffered a traumatic lisfranc fracture in March 2014 and underwent operative reconstruction. He had no relevant medical history and was not receiving concomitant medications. He had undergone anterior cruciate ligament reconstruction in 2008. The patient reported restricted training due to pain (score 7/10), stiffness (score 9/10) and poor function, as well as pain when changing direction and running on consecutive days. In July 2014 he started using TDT 064 twice daily at the recommended dose. In November 2014 he noted reduced pain on running (score 2-3/10), decreased morning stiffness (score 4/10) and improved joint mobility and was able to return to competitive rugby without restrictions. No side effects of TDT 064 were reported. Conclusions: TDT 064 shows efficacy against exercise- and injury-induced joint pain, as well as that associated with osteoarthritis. It does not retard muscle soreness recovery after exercise compared with an oral NSAID, making it an alternative approach for the treatment of joint pain during and post-exercise. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=exercise" title="exercise">exercise</a>, <a href="https://publications.waset.org/abstracts/search?q=joint%20pain" title=" joint pain"> joint pain</a>, <a href="https://publications.waset.org/abstracts/search?q=TDT%20064" title=" TDT 064"> TDT 064</a>, <a href="https://publications.waset.org/abstracts/search?q=phospholipid%20vesicles" title=" phospholipid vesicles"> phospholipid vesicles</a> </p> <a href="https://publications.waset.org/abstracts/22738/ultra-deformable-drug-free-sequessome-vesicles-tdt-064-for-the-treatment-of-joint-pain-following-exercise-a-case-report-and-clinical-data" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/22738.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">480</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">82</span> Surface Functionalized Biodegradable Polymersome for Targeted Drug Delivery</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Susmita%20Roy">Susmita Roy</a>, <a href="https://publications.waset.org/abstracts/search?q=Madhavan%20Nallani"> Madhavan Nallani</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In recent years' polymersomes, self-assembled polymeric vesicles emerge from block copolymers, have been widely investigated due to their enhance stability and unique advantageous properties compared to their phospholipid counterpart, liposomes, dendrimers, and micelles. It provides a distinctive platform for advanced therapeutics and the creation of complex (bio) catalytically active systems for research in Nanomedicine and synthetic biology. Inspired by nature, where compartmentalization of biological components is all ubiquitous, we are interested in developing a platform technology of self-assembled multifunctional compartments with applications in areas from targeted drug/gene delivery, biosensing, pharmaceutical to cosmetics. Polymersome surfaces can be a proper choice of derivatization with a controlled amount of functional groups. To achieve site-specific targeting of polymersomes, biological recognition motives can be attached to the polymersomes surface by standard bioconjugation techniques, (like esterification, amidation, thiol-maleimide coupling, click-chemistry routes or other coupling methods). Herein, we are developing easy going, one-step bioconjugation strategies for site-specific surface functionalized biodegradable polymeric and/or polymer-lipid hybrid vesicles for targeted drug delivery. Biodegradable polymer, polycaprolactone-b-polyethylene glycol (PCL-PEG), polylactic acid-b-polyethylene glycol (PLA-PEG) and phospholipid, 1-palmitoyl-2- oleoyl-sn-glycero-3-phosphocholine (POPC) has been widely used for numerous vesicle formulations. Some of these drug-loaded formulations are being tested on mice for controlled release. These surface functionalized polymersomes are also appropriate for membrane protein reconstitution/insertion, antibodies conjugation and various bioconjugation with diverse targeted molecules for controlled drug delivery. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=drug%20delivery" title="drug delivery">drug delivery</a>, <a href="https://publications.waset.org/abstracts/search?q=membrane%20protein" title=" membrane protein"> membrane protein</a>, <a href="https://publications.waset.org/abstracts/search?q=polymersome" title=" polymersome"> polymersome</a>, <a href="https://publications.waset.org/abstracts/search?q=surface%20modification" title=" surface modification"> surface modification</a> </p> <a href="https://publications.waset.org/abstracts/72971/surface-functionalized-biodegradable-polymersome-for-targeted-drug-delivery" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/72971.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">154</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">81</span> Bullous Pyoderma Gangrenosum in a Patient with Anti-Phospholipid Syndrome: A Case Report and Literature Review</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yousef%20Alwashahi">Yousef Alwashahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20Almoqbali"> Ahmed Almoqbali</a>, <a href="https://publications.waset.org/abstracts/search?q=Mayar%20Albahrani"> Mayar Albahrani</a>, <a href="https://publications.waset.org/abstracts/search?q=Asma%20Alajmi"> Asma Alajmi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> We report a rare case of a 49-year-old Omani woman who is a known case of primary anti-phospholipid syndrome, glucose-6-phosphate dehydrogenase deficiency, and iron deficiency anaemia. During cannulation, she was found to develop bulla that progressed to ulcerations. With chronicity and recurrent abscess formation that usually increase after surgical intervention, a pathergy phenomenon was postulated. High suspicion of pyoderma gangrenosum was considered. Fortunately, the rapid progression of the disease was slowed down with corticosteroids, cyclosporin, and biological agents. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anti-phospholipid%20syndrome" title="anti-phospholipid syndrome">anti-phospholipid syndrome</a>, <a href="https://publications.waset.org/abstracts/search?q=pyoderma%20gangrenosum" title=" pyoderma gangrenosum"> pyoderma gangrenosum</a>, <a href="https://publications.waset.org/abstracts/search?q=bullous%20pyoderma%20gangrenosum" title=" bullous pyoderma gangrenosum"> bullous pyoderma gangrenosum</a>, <a href="https://publications.waset.org/abstracts/search?q=pathergy" title=" pathergy"> pathergy</a>, <a href="https://publications.waset.org/abstracts/search?q=pathergy%20phenomenon" title=" pathergy phenomenon"> pathergy phenomenon</a> </p> <a href="https://publications.waset.org/abstracts/161750/bullous-pyoderma-gangrenosum-in-a-patient-with-anti-phospholipid-syndrome-a-case-report-and-literature-review" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/161750.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">91</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">80</span> Nanoparticles-Protein Hybrid-Based Magnetic Liposome </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Amlan%20Kumar%20Das">Amlan Kumar Das</a>, <a href="https://publications.waset.org/abstracts/search?q=Avinash%20Marwal"> Avinash Marwal</a>, <a href="https://publications.waset.org/abstracts/search?q=Vikram%20Pareek"> Vikram Pareek</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Liposome plays an important role in medical and pharmaceutical science as e.g. nano scale drug carriers. Liposomes are vesicles of varying size consisting of a spherical lipid bilayer and an aqueous inner compartment. Magnet-driven liposome used for the targeted delivery of drugs to organs and tissues1. These liposome preparations contain encapsulated drug components and finely dispersed magnetic particles. Liposomes are vesicles of varying size consisting of a spherical lipid bilayer and an aqueous inner compartment that are generated in vitro. These are useful in terms of biocompatibility, biodegradability, and low toxicity, and can control biodistribution by changing the size, lipid composition, and physical characteristics2. Furthermore, liposomes can entrap both hydrophobic and hydrophilic drugs and are able to continuously release the entrapped substrate, thus being useful drug carriers. Magnetic liposomes (MLs) are phospholipid vesicles that encapsulate magneticor paramagnetic nanoparticles. They are applied as contrast agents for magnetic resonance imaging (MRI)3. The biological synthesis of nanoparticles using plant extracts plays an important role in the field of nanotechnology4. Green-synthesized magnetite nanoparticles-protein hybrid has been produced by treating Iron (III)/Iron(II) chloride with the leaf extract of Dhatura Inoxia. The phytochemicals present in the leaf extracts act as a reducing as well stabilizing agents preventing agglomeration, which include flavonoids, phenolic compounds, cardiac glycosides, proteins and sugars. The magnetite nanoparticles-protein hybrid has been trapped inside the aqueous core of the liposome prepared by reversed phase evaporation (REV) method using oleic and linoleic acid which has been shown to be driven under magnetic field confirming the formation magnetic liposome (ML). Chemical characterization of stealth magnetic liposome has been performed by breaking the liposome and release of magnetic nanoparticles. The presence iron has been confirmed by colour complex formation with KSCN and UV-Vis study using spectrophotometer Cary 60, Agilent. This magnet driven liposome using nanoparticles-protein hybrid can be a smart vesicles for the targeted drug delivery. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=nanoparticles-protein%20hybrid" title="nanoparticles-protein hybrid">nanoparticles-protein hybrid</a>, <a href="https://publications.waset.org/abstracts/search?q=magnetic%20liposome" title=" magnetic liposome"> magnetic liposome</a>, <a href="https://publications.waset.org/abstracts/search?q=medical" title=" medical"> medical</a>, <a href="https://publications.waset.org/abstracts/search?q=pharmaceutical%20science" title=" pharmaceutical science"> pharmaceutical science</a> </p> <a href="https://publications.waset.org/abstracts/15000/nanoparticles-protein-hybrid-based-magnetic-liposome" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/15000.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">248</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">79</span> Biodegradable Polymeric Vesicles Containing Magnetic Nanoparticles, Quantum Dots and Anticancer Drugs for Drug Delivery and Imaging</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Fei%20Ye">Fei Ye</a>, <a href="https://publications.waset.org/abstracts/search?q=%C3%85sa%20Barrefelt"> Åsa Barrefelt</a>, <a href="https://publications.waset.org/abstracts/search?q=Manuchehr%20Abedi-Valugerdi"> Manuchehr Abedi-Valugerdi</a>, <a href="https://publications.waset.org/abstracts/search?q=Khalid%20M.%20Abu-Salah"> Khalid M. Abu-Salah</a>, <a href="https://publications.waset.org/abstracts/search?q=Salman%20A.%20Alrokayan"> Salman A. Alrokayan</a>, <a href="https://publications.waset.org/abstracts/search?q=Mamoun%20Muhammed"> Mamoun Muhammed</a>, <a href="https://publications.waset.org/abstracts/search?q=Moustapha%20Hassan"> Moustapha Hassan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> With appropriate encapsulation in functional nanoparticles drugs are more stable in physiological environment and the kinetics of the drug can be more carefully controlled and monitored. Furthermore, targeted drug delivery can be developed to improve chemotherapy in cancer treatment, not only by enhancing intracellular uptake by target cells but also by reducing the adverse effects in non-target organs. Inorganic imaging agents, delivered together with anti-cancer drugs, enhance the local imaging contrast and provide precise diagnosis as well as evaluation of therapy efficacy. We have developed biodegradable polymeric vesicles as a nanocarrier system for multimodal bio-imaging and anticancer drug delivery. The poly (lactic-co-glycolic acid) PLGA) vesicles were fabricated by encapsulating inorganic imaging agents of superparamagnetic iron oxide nanoparticles (SPION), manganese-doped zinc sulfide (MN:ZnS) quantum dots (QDs) and the anticancer drug busulfan into PLGA nanoparticles via an emulsion-evaporation method. T2-weighted magnetic resonance imaging (MRI) of PLGA-SPION-Mn:ZnS phantoms exhibited enhanced negative contrast with r2 relaxivity of approximately 523 s-1 mM-1 Fe. Murine macrophage (J774A) cellular uptake of PLGA vesicles started fluorescence imaging at 2 h and reached maximum intensity at 24 h incubation. The drug delivery ability PLGA vesicles was demonstrated in vitro by release of busulfan. PLGA vesicles degradation was studied in vitro, showing that approximately 32% was degraded into lactic and glycolic acid over a period of 5 weeks. The biodistribution of PLGA vesicles was investigated in vivo by MRI in a rat model. Change of contrast in the liver could be visualized by MRI after 7 min and maximal signal loss detected after 4 h post-injection of PLGA vesicles. Histological studies showed that the presence of PLGA vesicles in organs was shifted from the lungs to the liver and spleen over time. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=biodegradable%20polymers" title="biodegradable polymers">biodegradable polymers</a>, <a href="https://publications.waset.org/abstracts/search?q=multifunctional%20nanoparticles" title=" multifunctional nanoparticles"> multifunctional nanoparticles</a>, <a href="https://publications.waset.org/abstracts/search?q=quantum%20dots" title=" quantum dots"> quantum dots</a>, <a href="https://publications.waset.org/abstracts/search?q=anticancer%20drugs" title=" anticancer drugs"> anticancer drugs</a> </p> <a href="https://publications.waset.org/abstracts/29159/biodegradable-polymeric-vesicles-containing-magnetic-nanoparticles-quantum-dots-and-anticancer-drugs-for-drug-delivery-and-imaging" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/29159.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">472</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">78</span> Fabric Softener Deposition on Cellulose Nanocrystals and Cotton Fibers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Evdokia%20K.%20Oikonomou">Evdokia K. Oikonomou</a>, <a href="https://publications.waset.org/abstracts/search?q=Nikolay%20Christov"> Nikolay Christov</a>, <a href="https://publications.waset.org/abstracts/search?q=Galder%20Cristobal"> Galder Cristobal</a>, <a href="https://publications.waset.org/abstracts/search?q=Graziana%20Messina"> Graziana Messina</a>, <a href="https://publications.waset.org/abstracts/search?q=Giovani%20Marletta"> Giovani Marletta</a>, <a href="https://publications.waset.org/abstracts/search?q=Laurent%20Heux"> Laurent Heux</a>, <a href="https://publications.waset.org/abstracts/search?q=Jean-Francois%20Berret"> Jean-Francois Berret</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Fabric softeners are aqueous formulations that contain ~10 wt. % double tailed cationic surfactants. Here, a formulation in which 50% surfactant was replaced with low quantities of natural guar polymers was developed. Thanks to the reduced surfactant quantity this product has less environmental impact while the guars presence was found to maintain the product’s performance. The objective of this work is to elucidate the effect of the guar polymers on the softener deposition and the adsorption mechanism on the cotton surface. The surfactants in these formulations are assembled into large distributed (0.1 – 1 µm) vesicles that are stable in the presence of guars and upon dilution. The effect of guars on the vesicles adsorption on cotton was first estimated by using cellulose nanocrystals (CNC) as a stand-in for cotton. The dispersion of CNC in water permits to follow the interaction between the vesicles, guars, and CNC in the bulk. It was found that guars enhance the deposition on CNC and that the vesicles are deposited intactly on the fibers driven by electrostatics. The mechanism of the vesicles/guars adsorption on cellulose fibers was identified by quartz crystal microbalance with dissipation monitoring. It was found that the guars increase the surfactant deposited quantity, in agreement with the results in the bulk. Also, the structure of the adsorbed surfactant on the fibers' surfaces (vesicle or bilayer) was influenced by the guars presence. Deposition studies on cotton fabrics were also conducted. Attenuated total reflection and scanning electron microscopy were used to study the effect of the polymers on this deposition. Finally, fluorescent microscopy was used to follow the adsorption of surfactant vesicles, labeled with a fluorescent dye, on cotton fabrics in water. It was found that, in the presence or not of polymers, the surfactant vesicles are adsorbed on fiber maintaining their vesicular structure in water (supported vesicular bilayer structure). The guars influence this process. However, upon drying the vesicles are transformed into bilayers and eventually wrap the fibers (supported lipid bilayer structure). This mechanism is proposed for the adsorption of vesicular conditioner on cotton fiber and can be affected by the presence of polymers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=cellulose%20nanocrystals" title="cellulose nanocrystals">cellulose nanocrystals</a>, <a href="https://publications.waset.org/abstracts/search?q=cotton%20fibers" title=" cotton fibers"> cotton fibers</a>, <a href="https://publications.waset.org/abstracts/search?q=fabric%20softeners" title=" fabric softeners"> fabric softeners</a>, <a href="https://publications.waset.org/abstracts/search?q=guar%20polymers" title=" guar polymers"> guar polymers</a>, <a href="https://publications.waset.org/abstracts/search?q=surfactant%20vesicles" title=" surfactant vesicles"> surfactant vesicles</a> </p> <a href="https://publications.waset.org/abstracts/92386/fabric-softener-deposition-on-cellulose-nanocrystals-and-cotton-fibers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/92386.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">180</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">77</span> Medicinal Plants and Arbuscular mycorrhizal Colonization</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ammani%20K.">Ammani K.</a>, <a href="https://publications.waset.org/abstracts/search?q=Glory%20M."> Glory M.</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Demands of traditional herbal medicines are increasing day by day over the world. Considering the growing demand of medicinal plants in curative treatments and the role of VAM fungi in augmentation of the production of active secondary metabolites by the medicinal plants, the present work has been undertaken to survey the mycorrhizal status in 30 different medicinal plants belonging to various families from Krishna district, Andhra Pradesh. The roots were collected carefully and stained by the Phillips & Hayman technique. Basing on the occurrence of vesicles and arbuscules, categorized into four grades; Excellent: mycelia, vesicles or arbuscules present more than 75% of root bits, Good: mycelia, vesicles or arbuscules present 50-75% in surface of root bits, moderate: mycelia, vesicles or arbuscules present 25-50% in surface of root bits, and poor: mycelia, vesicles or arbuscules present 1-25% in surface of root bits. The study reveals that the roots of all plants were colonized by AM fungi. Percentage of root colonization by AM fungi was more in Aloe vera, Phylanthus emblica, Azadiracta indica and least in plants such as Aerva lanata, Vinca rosea, Crotalaria verrucosa among the 30 medicinal plants in present study. The enhancement of growth and vigour and increased production of bioactive compounds of the medicinal plants is desirable which may be achieved by inoculation of the roots with Arbuscular mycorrhizal fungi. There is a steady increase in the cultivation of medicinal plants to maintain a steady supply to support the increasing demand but corresponding researches of VAM fungi and their association in medicinal plants have received very little attention as compared to the studies on forest species and field crops. So a vast research on this field is necessary for a better tomorrow. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arbuscular%20mycorrhizae" title="Arbuscular mycorrhizae">Arbuscular mycorrhizae</a>, <a href="https://publications.waset.org/abstracts/search?q=colonization" title=" colonization"> colonization</a>, <a href="https://publications.waset.org/abstracts/search?q=categories" title=" categories"> categories</a>, <a href="https://publications.waset.org/abstracts/search?q=medicinal%20plants" title=" medicinal plants"> medicinal plants</a> </p> <a href="https://publications.waset.org/abstracts/35465/medicinal-plants-and-arbuscular-mycorrhizal-colonization" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35465.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">402</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">76</span> Unveiling the Self-Assembly Behavior and Salt-Induced Morphological Transition of Double PEG-Tailed Unconventional Amphiphiles</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rita%20Ghosh">Rita Ghosh</a>, <a href="https://publications.waset.org/abstracts/search?q=Joykrishna%20%20Dey"> Joykrishna Dey</a> </p> <p class="card-text"><strong>Abstract:</strong></p> PEG-based amphiphiles are of tremendous importance for its widespread applications in pharmaceutics, household purposes, and drug delivery. Previously, a number of single PEG-tailed amphiphiles having significant applications have been reported from our group. Therefore, it was of immense interest to explore the properties and application potential of PEG-based double tailed amphiphiles. Herein, for the first time, two novel double PEG-tailed amphiphiles having different PEG chain lengths have been developed. The self-assembly behavior of the newly developed amphiphiles in aqueous buffer (pH 7.0) was thoroughly investigated at 25 oC by a number of techniques including, 1H-NMR, and steady-state and time-dependent fluorescence spectroscopy, dynamic light scattering, transmission electron microscopy, atomic force microscopy, and isothermal titration calorimetry. Despite having two polar PEG chains both molecules were found to have strong tendency to self-assemble in aqueous buffered solution above a very low concentration. Surprisingly, the amphiphiles were shown to form stable vesicles spontaneously at room temperature without any external stimuli. The results of calorimetric measurements showed that the vesicle formation is driven by the hydrophobic effect (positive entropy change) of the system, which is associated with the helix-to-random coil transition of the PEG chain. The spectroscopic data confirmed that the bilayer membrane of the vesicles is constituted by the PEG chains of the amphiphilic molecule. Interestingly, the vesicles were also found to exhibit structural transitions upon addition of salts in solution. These properties of the vesicles enable them as potential candidate for drug delivery. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=double-tailed%20amphiphiles" title="double-tailed amphiphiles">double-tailed amphiphiles</a>, <a href="https://publications.waset.org/abstracts/search?q=fluorescence" title=" fluorescence"> fluorescence</a>, <a href="https://publications.waset.org/abstracts/search?q=microscopy" title=" microscopy"> microscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=PEG" title=" PEG"> PEG</a>, <a href="https://publications.waset.org/abstracts/search?q=vesicles" title=" vesicles"> vesicles</a> </p> <a href="https://publications.waset.org/abstracts/122452/unveiling-the-self-assembly-behavior-and-salt-induced-morphological-transition-of-double-peg-tailed-unconventional-amphiphiles" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/122452.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">117</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">75</span> Double Liposomes Based Dual Drug Delivery System for Effective Eradication of Helicobacter pylori</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Yuvraj%20Singh%20Dangi">Yuvraj Singh Dangi</a>, <a href="https://publications.waset.org/abstracts/search?q=Brajesh%20Kumar%20Tiwari"> Brajesh Kumar Tiwari</a>, <a href="https://publications.waset.org/abstracts/search?q=Ashok%20Kumar%20Jain"> Ashok Kumar Jain</a>, <a href="https://publications.waset.org/abstracts/search?q=Kamta%20Prasad%20Namdeo"> Kamta Prasad Namdeo</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The potential use of liposomes as drug carriers by i.v. injection is limited by their low stability in blood stream. Firstly, phospholipid exchange and transfer to lipoproteins, mainly HDL destabilizes and disintegrates liposomes with subsequent loss of content. To avoid the pain associated with injection and to obtain better patient compliance studies concerning various dosage forms, have been developed. Conventional liposomes (unilamellar and multilamellar) have certain drawbacks like low entrapment efficiency, stability and release of drug after single breach in external membrane, have led to the new type of liposomal systems. The challenge has been successfully met in the form of Double Liposomes (DL). DL is a recently developed type of liposome, consisting of smaller liposomes enveloped in lipid bilayers. The outer lipid layer of DL can protect inner liposomes against various enzymes, therefore DL was thought to be more effective than ordinary liposomes. This concept was also supported by in vitro release characteristics i.e. DL formation inhibited the release of drugs encapsulated in inner liposomes. DL consists of several small liposomes encapsulated in large liposomes, i.e., multivesicular vesicles (MVV), therefore, DL should be discriminated from ordinary classification of multilamellar vesicles (MLV), large unilamellar vesicles (LUV), small unilamellar vesicles (SUV). However, for these liposomes, the volume of inner phase is small and loading volume of water-soluble drugs is low. In the present study, the potential of phosphatidylethanolamine (PE) lipid anchored double liposomes (DL) to incorporate two drugs in a single system is exploited as a tool to augment the H. pylori eradication rate. Preparation of DL involves two steps, first formation of primary (inner) liposomes by thin film hydration method containing one drug, then addition of suspension of inner liposomes on thin film of lipid containing the other drug. The success of formation of DL was characterized by optical and transmission electron microscopy. Quantitation of DL-bacterial interaction was evaluated in terms of percent growth inhibition (%GI) on reference strain of H. pylori ATCC 26695. To confirm specific binding efficacy of DL to H. pylori PE surface receptor we performed an agglutination assay. Agglutination in DL treated H. pylori suspension suggested selectivity of DL towards the PE surface receptor of H. pylori. Monotherapy is generally not recommended for treatment of a H. pylori infection due to the danger of development of resistance and unacceptably low eradication rates. Therefore, combination therapy with amoxicillin trihydrate (AMOX) as anti-H. pylori agent and ranitidine bismuth citrate (RBC) as antisecretory agent were selected for the study with an expectation that this dual-drug delivery approach will exert acceptable anti-H. pylori activity. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Helicobacter%20pylorI" title="Helicobacter pylorI">Helicobacter pylorI</a>, <a href="https://publications.waset.org/abstracts/search?q=amoxicillin%20trihydrate" title=" amoxicillin trihydrate"> amoxicillin trihydrate</a>, <a href="https://publications.waset.org/abstracts/search?q=Ranitidine%20Bismuth%20citrate" title=" Ranitidine Bismuth citrate"> Ranitidine Bismuth citrate</a>, <a href="https://publications.waset.org/abstracts/search?q=phosphatidylethanolamine" title=" phosphatidylethanolamine"> phosphatidylethanolamine</a>, <a href="https://publications.waset.org/abstracts/search?q=multi%20vesicular%20systems" title=" multi vesicular systems"> multi vesicular systems</a> </p> <a href="https://publications.waset.org/abstracts/56355/double-liposomes-based-dual-drug-delivery-system-for-effective-eradication-of-helicobacter-pylori" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/56355.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">207</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">74</span> Synthesis and Characterization of Novel Hollow Silica Particle through DODAB Vesicle Templating</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Eun%20Ju%20Park">Eun Ju Park</a>, <a href="https://publications.waset.org/abstracts/search?q=Wendy%20Rusli"> Wendy Rusli</a>, <a href="https://publications.waset.org/abstracts/search?q=He%20Tao"> He Tao</a>, <a href="https://publications.waset.org/abstracts/search?q=Alexander%20M.%20Van%20Herk"> Alexander M. Van Herk</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanggu%20Kim"> Sanggu Kim</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Hollow micro-/nano- structured materials have proven to be promising in wide range of applications, such as catalysis, drug delivery and controlled release, biotechnology, and personal and consumer care. Hollow sphere structures can be obtained through various templating approaches; colloid templates, emulsion templates, multi-surfactant templates, and single crystal templates. Vesicles are generally the self-directed assemblies of amphiphilic molecules including cationic, anionic, and cationic surfactants in aqueous solutions. The directed silica capsule formations were performed at the surface of dioctadecyldimethylammoniumbromide(DODAB) bilayer vesicles as soft template. The size of DODAB bilayer vesicles could be tuned by extrusion of a preheated dispersion of DODAB. The synthesized hollow silica particles were characterized by conventional TEM, cryo-TEM and SEM to determine the morphology and structure of particles and dynamic light scattering (DLS) method to measure the particle size and particle size distribution. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=characterization" title="characterization">characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=DODAB" title=" DODAB"> DODAB</a>, <a href="https://publications.waset.org/abstracts/search?q=hollow%20silica%20particle" title=" hollow silica particle"> hollow silica particle</a>, <a href="https://publications.waset.org/abstracts/search?q=synthesis" title=" synthesis"> synthesis</a>, <a href="https://publications.waset.org/abstracts/search?q=vesicle" title=" vesicle"> vesicle</a> </p> <a href="https://publications.waset.org/abstracts/35402/synthesis-and-characterization-of-novel-hollow-silica-particle-through-dodab-vesicle-templating" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/35402.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">307</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">73</span> Regulating Nanocarrier and Mononuclear Phagocyte System Interactions through Esomeprazole-Based Preconditioning Strategy</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Zakia%20Belhadj">Zakia Belhadj</a>, <a href="https://publications.waset.org/abstracts/search?q=Bing%20He"> Bing He</a>, <a href="https://publications.waset.org/abstracts/search?q=Hua%20Zhang"> Hua Zhang</a>, <a href="https://publications.waset.org/abstracts/search?q=Xueqing%20Wang"> Xueqing Wang</a>, <a href="https://publications.waset.org/abstracts/search?q=Wenbing%20Dai"> Wenbing Dai</a>, <a href="https://publications.waset.org/abstracts/search?q=Qiang%20Zhang"> Qiang Zhang</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Mononuclear phagocyte system (MPS) forms an abominable obstacle hampering the tumor delivery efficiency of nanoparticles. Passively targeted nanocarriers have received clinical approval over the past 20 years. However, none of the actively targeted nanocarriers have entered clinical trials. Thus it is important to endue effective targeting ability to actively targeted approaches by overcoming biological barriers to nanoparticle drug delivery. Here, it presents that an Esomeprazole-based preconditioning strategy for regulating nanocarrier-MPS interaction to substantially prolong circulation time and enhance tumor targeting of nanoparticles. In vitro, the clinically approved proton pump inhibitor Esomeprazole “ESO” was demonstrated to reduce interactions between macrophages and subsequently injected targeted vesicles by interfering with their lysosomal trafficking. Of note, in vivo studies demonstrated that ESO pretreatment greatly decreased the liver and spleen uptake of c(RGDm7)-modified vesicles, highly enhanced their tumor accumulation, thereby provided superior therapeutic efficacy of c(RGDm7)-modified vesicles co-loaded with Doxorubicin (DOX) and Gefitinib (GE). This MPS-preconditioning strategy using ESO provides deeper insights into regulating nanoparticles interaction with the phagocytic system and enhancing their cancer cells' accessibility for anticancer therapy. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=esomeprazole%20%28ESO%29" title="esomeprazole (ESO)">esomeprazole (ESO)</a>, <a href="https://publications.waset.org/abstracts/search?q=mononuclear%20phagocyte%20system%20%28MPS%29" title=" mononuclear phagocyte system (MPS)"> mononuclear phagocyte system (MPS)</a>, <a href="https://publications.waset.org/abstracts/search?q=preconditioning%20strategy" title=" preconditioning strategy"> preconditioning strategy</a>, <a href="https://publications.waset.org/abstracts/search?q=targeted%20lipid%20vesicles" title=" targeted lipid vesicles"> targeted lipid vesicles</a> </p> <a href="https://publications.waset.org/abstracts/139048/regulating-nanocarrier-and-mononuclear-phagocyte-system-interactions-through-esomeprazole-based-preconditioning-strategy" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139048.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">176</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">72</span> Effect of Non-Ionic Surfactants on in vitro Release of Ketorolactromethamine</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ajay%20Aggarwal">Ajay Aggarwal</a>, <a href="https://publications.waset.org/abstracts/search?q=Kamal%20Saroha"> Kamal Saroha</a>, <a href="https://publications.waset.org/abstracts/search?q=Sanju%20Nanda"> Sanju Nanda</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Niosomes or non-ionic surfactant vesicles are microscopic lamellar structures formed on admixture of non-ionic surfactant of the alkyl or dialkyl polyglycerol ether class and cholesterol with subsequent hydration in aqueous media. They are vesicular systems similar to liposomes that can be used as carriers of amphiphilic and lipophilic drugs. Entrapment efficiency was found to be higher in case of niosome prepared with span60 than niosome prepared with tween. The amount of release was found to be in order of Span20>Tween60>Tween20>Span60. As the concentration of surfactant is increased in vitro release was increased due to high entrapment. The stability study of optimized batch revealed that particle size was increased after 3months on increasing the temperature. On the other hand entrapment efficiency was decreased on increasing the temperature. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=niosomes" title="niosomes">niosomes</a>, <a href="https://publications.waset.org/abstracts/search?q=vesicles" title=" vesicles"> vesicles</a>, <a href="https://publications.waset.org/abstracts/search?q=span" title=" span"> span</a>, <a href="https://publications.waset.org/abstracts/search?q=tween" title=" tween"> tween</a>, <a href="https://publications.waset.org/abstracts/search?q=in%20vitro%20release" title=" in vitro release"> in vitro release</a> </p> <a href="https://publications.waset.org/abstracts/41358/effect-of-non-ionic-surfactants-on-in-vitro-release-of-ketorolactromethamine" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/41358.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">357</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">71</span> The Molecular Mechanism of Vacuolar Function in Yeast Cell Homeostasis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Chang-Hui%20Shen">Chang-Hui Shen</a>, <a href="https://publications.waset.org/abstracts/search?q=Paulina%20Konarzewska"> Paulina Konarzewska</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Cell homeostasis is regulated by vacuolar activity and it has been shown that lipid composition of the vacuole plays an important role in vacuolar function. The major phosphoinositide species present in the vacuolar membrane include phosphatidylinositol 3,5-biphosphate (PI(3,5)P₂) which is generated from PI(3)P controlled by Fab1p. Deletion of FAB1 gene reduce the synthesis of PI(3,5)P₂ and thus result in enlarged or fragmented vacuoles, with neutral vacuolar pH due to reduced vacuolar H⁺-ATPase activity. These mutants also exhibited poor growth at high extracellular pH and in the presence of CaCl₂. Conversely, VPS34 regulates the synthesis of PI(3)P from phosphatidylinositol (PI), and the lack of Vps34p results in the reduction of vacuolar activity. Although the cellular observations are clear, it is still unknown about the molecular mechanism between the phospholipid biosynthesis pathway and vacuolar activity. Since both VPS34 and FAB1 are important in vacuolar activity, we hypothesize that the molecular mechanism of vacuolar function might be regulated by the transcriptional regulators of phospholipid biosynthesis. In this study, we study the role of the major phospholipid biosynthesis transcription factor, INO2, in the regulation of vacuolar activity. We first performed qRT-PCR to examine the effect of Ino2p on the expression of VPS34 and FAB1. Our results showed that VPS34 was upregulated in the presence of inositol for both WT and ino2Δ cells. However, FAB1 was only upregulated significantly in ino2Δ cells. This indicated that Ino2p might be the negative regulator for FAB1 expression. Next, growth sensitivity experiment showed that WT, vma3Δ, and ino2Δ grew well in growth medium buffered to pH 5.5 containing 10 mM CaCl₂. As cells were switched to growth medium buffered to pH 7 containing CaCl₂ WT, ino2Δ and opi1Δ showed growth reduction, whereas vma3Δ was completely nonviable. As the concentration of CaCl₂ was increased to 60 mM, ino2Δ cells showed moderate growth reduction compared to WT. This result suggests that ino2Δ cells have better vacuolar activity. Microscopic analysis and vacuolar acidification were employed to further elucidate the importance of INO2 in vacuolar homeostasis. Analysis of vacuolar morphology indicated that WT and vma3Δ cells displayed vacuoles that occupied a small area of the cell when grown in media buffered to pH 5.5. Whereas, ino2Δ displayed fragmented vacuoles. On the other hand, all strains grown in media buffered to pH 7, exhibited enlarged vacuoles that occupied most of the cell’s surface. This indicated that the presence of INO2 may play negative effect in vacuolar morphology when cells are grown in media buffered to pH 5.5. Furthermore, vacuolar acidification assay showed that only vma3Δ cells displayed notably less acidic vacuoles as cells were grown in media buffered to pH 5.5 and pH 7. Whereas, ino2Δ cells displayed more acidic pH compared to WT at pH7. Taken together, our results demonstrated the molecular mechanism of the vacuolar activity regulated by the phospholipid biosynthesis transcription factors Ino2p. Ino2p negatively regulates vacuolar activity through the expression of FAB1. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=vacuole" title="vacuole">vacuole</a>, <a href="https://publications.waset.org/abstracts/search?q=phospholipid" title=" phospholipid"> phospholipid</a>, <a href="https://publications.waset.org/abstracts/search?q=homeostasis" title=" homeostasis"> homeostasis</a>, <a href="https://publications.waset.org/abstracts/search?q=Ino2p" title=" Ino2p"> Ino2p</a>, <a href="https://publications.waset.org/abstracts/search?q=FAB1" title=" FAB1"> FAB1</a> </p> <a href="https://publications.waset.org/abstracts/94378/the-molecular-mechanism-of-vacuolar-function-in-yeast-cell-homeostasis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/94378.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">127</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">70</span> Computational Characterization of Electronic Charge Transfer in Interfacial Phospholipid-Water Layers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Samira%20Baghbanbari">Samira Baghbanbari</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20B.%20P.%20Lever"> A. B. P. Lever</a>, <a href="https://publications.waset.org/abstracts/search?q=Payam%20S.%20Shabestari"> Payam S. Shabestari</a>, <a href="https://publications.waset.org/abstracts/search?q=Donald%20Weaver"> Donald Weaver</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Existing signal transmission models, although undoubtedly useful, have proven insufficient to explain the full complexity of information transfer within the central nervous system. The development of transformative models will necessitate a more comprehensive understanding of neuronal lipid membrane electrophysiology. Pursuant to this goal, the role of highly organized interfacial phospholipid-water layers emerges as a promising case study. A series of phospholipids in neural-glial gap junction interfaces as well as cholesterol molecules have been computationally modelled using high-performance density functional theory (DFT) calculations. Subsequent 'charge decomposition analysis' calculations have revealed a net transfer of charge from phospholipid orbitals through the organized interfacial water layer before ultimately finding its way to cholesterol acceptor molecules. The specific pathway of charge transfer from phospholipid via water layers towards cholesterol has been mapped in detail. Cholesterol is an essential membrane component that is overrepresented in neuronal membranes as compared to other mammalian cells; given this relative abundance, its apparent role as an electronic acceptor may prove to be a relevant factor in further signal transmission studies of the central nervous system. The timescales over which this electronic charge transfer occurs have also been evaluated by utilizing a system design that systematically increases the number of water molecules separating lipids and cholesterol. Memory loss through hydrogen-bonded networks in water can occur at femtosecond timescales, whereas existing action potential-based models are limited to micro or nanosecond scales. As such, the development of future models that attempt to explain faster timescale signal transmission in the central nervous system may benefit from our work, which provides additional information regarding fast timescale energy transfer mechanisms occurring through interfacial water. The study possesses a dataset that includes six distinct phospholipids and a collection of cholesterol. Ten optimized geometric characteristics (features) were employed to conduct binary classification through an artificial neural network (ANN), differentiating cholesterol from the various phospholipids. This stems from our understanding that all lipids within the first group function as electronic charge donors, while cholesterol serves as an electronic charge acceptor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=charge%20transfer" title="charge transfer">charge transfer</a>, <a href="https://publications.waset.org/abstracts/search?q=signal%20transmission" title=" signal transmission"> signal transmission</a>, <a href="https://publications.waset.org/abstracts/search?q=phospholipids" title=" phospholipids"> phospholipids</a>, <a href="https://publications.waset.org/abstracts/search?q=water%20layers" title=" water layers"> water layers</a>, <a href="https://publications.waset.org/abstracts/search?q=ANN" title=" ANN"> ANN</a> </p> <a href="https://publications.waset.org/abstracts/175684/computational-characterization-of-electronic-charge-transfer-in-interfacial-phospholipid-water-layers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/175684.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">73</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">69</span> Formulation and Evaluation of Niosomes Containing an Antihypertensive Drug</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Sunil%20Kamboj">Sunil Kamboj</a>, <a href="https://publications.waset.org/abstracts/search?q=Suman%20Bala"> Suman Bala</a>, <a href="https://publications.waset.org/abstracts/search?q=Vipin%20Saini"> Vipin Saini</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Niosomes were formulated with an aim of enhancing the oral bioavailability of losartan potassium and formulated in different molar ratios of surfactant, cholesterol and dicetyl phosphate. The formulated niosomes were found in range of 54.98 µm to 107.85 µm in size. Formulations with 1:1 ratio of surfactant and cholesterol have shown maximum entrapment efficiencies. Niosomes with sorbitan monostearate showed maximum drug release and zero order release kinetics, at the end of 24 hours. The <em>in vivo</em> study has shown the significant enhancement in oral bioavailability of losartan potassium in rats, after a dose of 10 mg/kg. The average relative bioavailability in relation with pure drug solution was found 2.56, indicates more than two fold increase in oral bioavailability. A significant increment in MRT reflects the release retarding ability of the vesicles. In conclusion, niosomes could be a promising delivery of losartan potassium with improved oral bioavailability and prolonged release profiles. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=non-ionic%20surfactant%20vesicles" title="non-ionic surfactant vesicles">non-ionic surfactant vesicles</a>, <a href="https://publications.waset.org/abstracts/search?q=losartan%20potassium" title=" losartan potassium"> losartan potassium</a>, <a href="https://publications.waset.org/abstracts/search?q=oral%20bioavailability" title=" oral bioavailability"> oral bioavailability</a>, <a href="https://publications.waset.org/abstracts/search?q=controlled%20release" title=" controlled release"> controlled release</a> </p> <a href="https://publications.waset.org/abstracts/37426/formulation-and-evaluation-of-niosomes-containing-an-antihypertensive-drug" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/37426.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">354</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">68</span> Biophysical Features of Glioma-Derived Extracellular Vesicles as Potential Diagnostic Markers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abhimanyu%20Thakur">Abhimanyu Thakur</a>, <a href="https://publications.waset.org/abstracts/search?q=Youngjin%20Lee"> Youngjin Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Glioma is a lethal brain cancer whose early diagnosis and prognosis are limited due to the dearth of a suitable technique for its early detection. Current approaches, including magnetic resonance imaging (MRI), computed tomography (CT), and invasive biopsy for the diagnosis of this lethal disease, hold several limitations, demanding an alternative method. Recently, extracellular vesicles (EVs) have been used in numerous biomarker studies, majorly exosomes and microvesicles (MVs), which are found in most of the cells and biofluids, including blood, cerebrospinal fluid (CSF), and urine. Remarkably, glioma cells (GMs) release a high number of EVs, which are found to cross the blood-brain-barrier (BBB) and impersonate the constituents of parent GMs including protein, and lncRNA; however, biophysical properties of EVs have not been explored yet as a biomarker for glioma. We isolated EVs from cell culture conditioned medium of GMs and regular primary culture, blood, and urine of wild-type (WT)- and glioma mouse models, and characterized by nano tracking analyzer, transmission electron microscopy, immunogold-EM, and differential light scanning. Next, we measured the biophysical parameters of GMs-EVs by using atomic force microscopy. Further, the functional constituents of EVs were examined by FTIR and Raman spectroscopy. Exosomes and MVs-derived from GMs, blood, and urine showed distinction biophysical parameters (roughness, adhesion force, and stiffness) and different from that of regular primary glial cells, WT-blood, and -urine, which can be attributed to the characteristic functional constituents. Therefore, biophysical features can be potential diagnostic biomarkers for glioma. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=glioma" title="glioma">glioma</a>, <a href="https://publications.waset.org/abstracts/search?q=extracellular%20vesicles" title=" extracellular vesicles"> extracellular vesicles</a>, <a href="https://publications.waset.org/abstracts/search?q=exosomes" title=" exosomes"> exosomes</a>, <a href="https://publications.waset.org/abstracts/search?q=microvesicles" title=" microvesicles"> microvesicles</a>, <a href="https://publications.waset.org/abstracts/search?q=biophysical%20properties" title=" biophysical properties"> biophysical properties</a> </p> <a href="https://publications.waset.org/abstracts/131887/biophysical-features-of-glioma-derived-extracellular-vesicles-as-potential-diagnostic-markers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/131887.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">142</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">67</span> Resveratrol Incorporated Liposomes Prepared from Pegylated Phospholipids and Cholesterol</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mont%20Kumpugdee-Vollrath">Mont Kumpugdee-Vollrath</a>, <a href="https://publications.waset.org/abstracts/search?q=Khaled%20Abdallah"> Khaled Abdallah</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Liposomes and pegylated liposomes were widely used as drug delivery system in pharmaceutical field since a long time. However, in the former time, polyethylene glycol (PEG) was connected into phospholipid after the liposomes were already prepared. In this paper, we intend to study the possibility of applying phospholipids which already connected with PEG and then they were used to prepare liposomes. The model drug resveratrol was used because it can be applied against different diseases. Cholesterol was applied to stabilize the membrane of liposomes. The thin film technique in a laboratory scale was a preparation method. The liposomes were then characterized by nanoparticle tracking analysis (NTA), photon correlation spectroscopy (PCS) and light microscopic techniques. The stable liposomes can be produced and the particle sizes after filtration were in nanometers. The 2- and 3-chains-PEG-phospholipid (PL) caused in smaller particle size than the 4-chains-PEG-PL. Liposomes from PL 90G and cholesterol were stable during storage at 8 °C of 56 days because the particle sizes measured by PCS were almost not changed. There was almost no leakage of resveratrol from liposomes PL 90G with cholesterol after diffusion test in dialysis tube for 28 days. All liposomes showed the sustained release during measuring time of 270 min. The maximum release amount of 16-20% was detected with liposomes from 2- and 3-chains-PEG-PL. The other liposomes gave max. release amount of resveratrol only of 10%. The release kinetic can be explained by Korsmeyer-Peppas equation.  <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=liposome" title="liposome">liposome</a>, <a href="https://publications.waset.org/abstracts/search?q=NTA" title=" NTA"> NTA</a>, <a href="https://publications.waset.org/abstracts/search?q=resveratrol" title=" resveratrol"> resveratrol</a>, <a href="https://publications.waset.org/abstracts/search?q=pegylation" title=" pegylation"> pegylation</a>, <a href="https://publications.waset.org/abstracts/search?q=cholesterol" title=" cholesterol"> cholesterol</a> </p> <a href="https://publications.waset.org/abstracts/53135/resveratrol-incorporated-liposomes-prepared-from-pegylated-phospholipids-and-cholesterol" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/53135.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">185</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">66</span> Resveratrol-Phospholipid Complex for Sustained Delivery of Resveratrol via the Skin for the Treatment of Inflammatory Diseases</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Malay%20K.%20Das">Malay K. Das</a>, <a href="https://publications.waset.org/abstracts/search?q=Bhupen%20Kalita"> Bhupen Kalita</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The poor oral bioavailability of resveratrol (RSV) due to presystemic metabolism can be avoided via dermal route of administration. The hydrophilic-lipophilic nature of resveratrol-phospholipid complex (RSVPs) favors the delivery of resveratrol via the skin. The RSVPs embedded polymeric patch with moderate adhesiveness was developed for dermal application for sustained anti-inflammatory effect. The prepared patches were evaluated for various physicochemical properties, surface morphology by SEM, TEM, and compatibility of patch components by FT-IR and DSC studies. The dermal flux of the optimized patch formulation was found to be at 4.28 ± 0.48 mg/cm2/24 h. The analysis of skin extract after permeation study revealed the presence of resveratrol, which confirmed the localization of RSVPs in the skin. The stability of RSVPs in the polymeric patch and the physiologic environment was confirmed by FE-SEM studies on the patches after drug release and skin permeation studies. The RSVPs particles released from the polymer matrix maintaining the structural integrity and permeate the keratinized horney layer of skin. The optimized patch formulation showed sustained anti-inflammatory effect (84.10% inhibition of inflammation at 24 h) in carrageenan-induced rat paw edema model compared to marketed diclofenac sodium gel (39.58% inhibition of inflammation at 24 h). The CLSM study confirmed the localization of RSVPs for a longer period, thus enabling drug targeting to the dermis for sustained anti-inflammatory effect. Histological studies with phase contrast trinocular microscope suggested no alteration of skin integrity and no evidence of the presence of inflammatory cells after exposure to the permeants. The patch was found to be safe for skin application as evaluated by Draize method for skin irritation scoring in a rabbit model. These results suggest the therapeutic efficacy of the developed patch in both acute and chronic inflammatory diseases. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=resveratrol-phospholipid%20complex" title="resveratrol-phospholipid complex">resveratrol-phospholipid complex</a>, <a href="https://publications.waset.org/abstracts/search?q=skin%20delivery" title=" skin delivery"> skin delivery</a>, <a href="https://publications.waset.org/abstracts/search?q=sustained%20anti-inflammatory%20effect" title=" sustained anti-inflammatory effect"> sustained anti-inflammatory effect</a>, <a href="https://publications.waset.org/abstracts/search?q=inflammatory%20diseases" title=" inflammatory diseases"> inflammatory diseases</a>, <a href="https://publications.waset.org/abstracts/search?q=dermal%20patch" title=" dermal patch"> dermal patch</a> </p> <a href="https://publications.waset.org/abstracts/52751/resveratrol-phospholipid-complex-for-sustained-delivery-of-resveratrol-via-the-skin-for-the-treatment-of-inflammatory-diseases" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/52751.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">230</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">65</span> Investigating the Suitability of Utilizing Lyophilized Gels to Improve the Stability of Ufasomes</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mona%20Hassan%20Aburahma">Mona Hassan Aburahma</a>, <a href="https://publications.waset.org/abstracts/search?q=Alaa%20Hamed%20Salama"> Alaa Hamed Salama </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Ufasomes “unsaturated fatty acids liposomes” are unique nano-sized self-assembled bilayered vesicles that can be easily created from the readily available unsaturated fatty acid. Ufasomes are formed due to weak associative interaction of the fully ionized and unionized fatty acids into bilayers structures. In the ufasomes constructs, the fatty acid molecules are oriented with their hydrocarbon tails directed toward the membrane interior and the carboxyl groups are in contact with water. Although ufasomes can be employed as a safe vesicular carrier for drugs, the extreme instability of their aqueous dispersions hinders their effective use in drug delivery field. Accordingly, in our study, lyophilized gels containing ufasomes were prepared using a simple assembling technique form the readily available oleic acid to overcome the colloidal instability of the ufasomes dispersions and convert them into accurate unit dosage forms. The influence of changing cholesterol percentage relative to oleic acid on the ufasomes vesicles were investigated using factorial design. The optimized oleic acid ufasomes comprised nanoscaled spherical vesicles. Scanning electron micrographs of the lyophilized gels revealed that the included ufasomes were intact, non-aggregating, and preserved their spherical morphology. Rheological characterization (viscosity and shear stress versus shear rate) of reconstituted ufasomal lyophilized gel ensured the ease of application. The capability of the ufasomes, included in the gel, to penetrate deep through the mucosa layers was illustrated using ex-vivo confocal laser imaging, thereby, highlighting the feasibility of stabilizing ufasomes using lyophilized gel platforms. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=ufasomes" title="ufasomes">ufasomes</a>, <a href="https://publications.waset.org/abstracts/search?q=lyophilized%20gel" title=" lyophilized gel"> lyophilized gel</a>, <a href="https://publications.waset.org/abstracts/search?q=confocal%20scanning%20microscopy" title=" confocal scanning microscopy"> confocal scanning microscopy</a>, <a href="https://publications.waset.org/abstracts/search?q=rheological%20characterization" title=" rheological characterization"> rheological characterization</a>, <a href="https://publications.waset.org/abstracts/search?q=oleic%20acid" title=" oleic acid "> oleic acid </a> </p> <a href="https://publications.waset.org/abstracts/21763/investigating-the-suitability-of-utilizing-lyophilized-gels-to-improve-the-stability-of-ufasomes" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/21763.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">408</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">64</span> Biosurfactant: A Greener Approach for Enhanced Concrete Rheology and Strength</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Olivia%20Anak%20Rayeg">Olivia Anak Rayeg</a>, <a href="https://publications.waset.org/abstracts/search?q=Clotilda%20Binti%20Petrus"> Clotilda Binti Petrus</a>, <a href="https://publications.waset.org/abstracts/search?q=Arnel%20Reanturco%20Ascotia"> Arnel Reanturco Ascotia</a>, <a href="https://publications.waset.org/abstracts/search?q=Ang%20Chung%20Huap"> Ang Chung Huap</a>, <a href="https://publications.waset.org/abstracts/search?q=Caroline%20Marajan"> Caroline Marajan</a>, <a href="https://publications.waset.org/abstracts/search?q=Rudy%20Tawie%20Joseph%20Sipi"> Rudy Tawie Joseph Sipi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Concrete is essential for global infrastructure, yet enhancing its rheology and strength in an environmentally sustainable manner remains a significant challenge. Conventional chemical admixtures often pose environmental and health risks. This study explores the use of a phospholipid biosurfactant, derived from Rhizopus oryzae, as an environmentally friendly admixture in concrete. Various concentrations of the biosurfactant were integrated into fresh concrete, partially replacing the water content. The inclusion of the biosurfactant markedly enhanced the workability of the concrete, as demonstrated by Vertical Slump, Slump Flow, and T50 tests. After a 28-day curing period, the concrete's mechanical properties were assessed through compressive strength and bonding tests. Results revealed that substituting up to 10% of the water with the biosurfactant not only improved workability but also significantly increased both compressive and flexural strength. These findings highlight the potential of phospholipid biosurfactant as a biodegradable and non-toxic alternative to traditional admixtures, enhancing both structural integrity and sustainability in concrete. This approach reduces environmental impact and production costs, marking a significant advancement in sustainable construction technology. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=concrete%20rheology" title="concrete rheology">concrete rheology</a>, <a href="https://publications.waset.org/abstracts/search?q=green%20admixture" title=" green admixture"> green admixture</a>, <a href="https://publications.waset.org/abstracts/search?q=fungal%20biosurfactant" title=" fungal biosurfactant"> fungal biosurfactant</a>, <a href="https://publications.waset.org/abstracts/search?q=phospholipids" title=" phospholipids"> phospholipids</a>, <a href="https://publications.waset.org/abstracts/search?q=rhizopus%20oryzae" title=" rhizopus oryzae"> rhizopus oryzae</a> </p> <a href="https://publications.waset.org/abstracts/186458/biosurfactant-a-greener-approach-for-enhanced-concrete-rheology-and-strength" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/186458.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">43</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">63</span> Natural Honey and Effect on the Activity of the Cells</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abujnah%20Dukali">Abujnah Dukali</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Natural honey was assessed in cell culture system for its anticancer activity. Human leukemic cell line HL 60 was treated with honey and cultured for 5 days and cytotoxicity was calculated by MTT assay. Honey showed cytotoxicity with CC50 value of 174.20 µg/ml. Radical modulation activities was assessed by lipid peroxidation assay using egg lecithin. Honey showed antioxidant activity with EC50 value of 159.73 µg/ml. In addition, treatment with HL60 cells also resulted in nuclear DNA fragmentation, as seen in agarose gel electrophoresis. This is a hallmark of cells undergoing apoptosis. Confirmation of apoptosis was performed by staining the cells with Annexin V and FACS analysis. Apoptosis is an active, genetically regulated disassembly of the cell form within. Disassembly creates changes in the phospholipid content of the cytoplasmic membrane outer leaflet. Phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. The human anticoagulant, annexin V, is a Ca2+-dependent phospholipid protein with a high affinity for PS. Annexin V labeled with fluorescein can identify apoptotic cells in the population It is a confirmatory test for apoptosis. Annexin V-positive cells were defined as apoptotic cells. Since honey shows both antioxidant activity and cytotoxicity at almost the same concentration, it can prevent the free radical induced cancer as prophylactic agent and kill the cancer cells by apoptotic process as a chemotherapeutic agent. Everyday intake of honey can prevent the cancer induction. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=anticancer" title="anticancer">anticancer</a>, <a href="https://publications.waset.org/abstracts/search?q=cells" title=" cells"> cells</a>, <a href="https://publications.waset.org/abstracts/search?q=DNA" title=" DNA"> DNA</a>, <a href="https://publications.waset.org/abstracts/search?q=honey" title=" honey"> honey</a> </p> <a href="https://publications.waset.org/abstracts/44196/natural-honey-and-effect-on-the-activity-of-the-cells" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/44196.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">206</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">62</span> Ultrastructural Characterization of Lipid Droplets of Rat Hepatocytes after Whole Body 60-Cobalt Gamma Radiation</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Ivna%20Moror%C3%B3">Ivna Mororó</a>, <a href="https://publications.waset.org/abstracts/search?q=Lise%20P.%20Lab%C3%A9jof"> Lise P. Labéjof</a>, <a href="https://publications.waset.org/abstracts/search?q=Stephanie%20Ribeiro"> Stephanie Ribeiro</a>, <a href="https://publications.waset.org/abstracts/search?q=Kely%20Almeida"> Kely Almeida</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Lipid droplets (LDs) are normally presented in greater or lesser number in the cytoplasm of almost all eukaryotic and some prokaryotic cells. They are independent organelles composed of a lipid ester core and a surface phospholipid monolayer. As a lipid storage form, they provide an available source of energy for the cell. Recently it was demonstrated that they play an important role in other many cellular processes. Among the many unresolved questions about them, it is not even known how LDs is formed, how lipids are recruited to LDs and how they interact with the other organelles. Excess fat in the organism is pathological and often associated with the development of some genetic, hormonal or behavioral diseases. The formation and accumulation of lipid droplets in the cytoplasm can be increased by exogenous physical or chemical agents. It is well known that ionizing radiation affects lipid metabolism resulting in increased lipogenesis in cells, but the details of this process are unknown. To better understand the mode of formation of LDs in liver cells, we investigate their ultrastructural morphology after irradiation. For that, Wistar rats were exposed to whole body gamma radiation from 60-cobalt at various single doses. Samples of the livers were processed for analysis under a conventional transmission electron microscope. We found that when compared to controls, morphological changes in liver cells were evident at the higher doses of radiation used. It was detected a great number of lipid droplets of different sizes and homogeneous content and some of them merged each other. In some cells, it was observed diffused LDs, not limited by a monolayer of phospholipids. This finding suggests that the phospholipid monolayer of the LDs was disrupted by ionizing radiation exposure that promotes lipid peroxydation of endo membranes. Thus the absence of the phospholipid monolayer may prevent the realization of some cellular activities as follow: - lipid exocytosis which requires the merging of LDs membrane with the plasma membrane; - the interaction of LDs with other membrane-bound organelles such as the endoplasmic reticulum (ER), the golgi and mitochondria and; - lipolysis of lipid esters contained in the LDs which requires the presence of enzymes located in membrane-bound organelles as ER. All these impediments can contribute to lipid accumulation in the cytoplasm and the development of diseases such as liver steatosis, cirrhosis and cancer. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=radiobiology" title="radiobiology">radiobiology</a>, <a href="https://publications.waset.org/abstracts/search?q=hepatocytes" title=" hepatocytes"> hepatocytes</a>, <a href="https://publications.waset.org/abstracts/search?q=lipid%20metabolism" title=" lipid metabolism"> lipid metabolism</a>, <a href="https://publications.waset.org/abstracts/search?q=transmission%20electron%20microscopy" title=" transmission electron microscopy"> transmission electron microscopy</a> </p> <a href="https://publications.waset.org/abstracts/43603/ultrastructural-characterization-of-lipid-droplets-of-rat-hepatocytes-after-whole-body-60-cobalt-gamma-radiation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/43603.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">314</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">61</span> An Insight into the Paddy Soil Denitrifying Bacteria and Their Relation with Soil Phospholipid Fatty Acid Profile</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Meenakshi%20Srivastava">Meenakshi Srivastava</a>, <a href="https://publications.waset.org/abstracts/search?q=A.%20K.%20Mishra"> A. K. Mishra</a> </p> <p class="card-text"><strong>Abstract:</strong></p> This study characterizes the metabolic versatility of denitrifying bacterial communities residing in the paddy soil using the GC-MS based Phospholipid Fatty Acid (PLFA) analyses simultaneously with nosZ gene based PCR-DGGE (Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis) and real time Q-PCR analysis. We have analyzed the abundance of nitrous oxide reductase (nosZ) genes, which was subsequently related to soil PLFA profile and DGGE based denitrifier community structure. Soil denitrifying bacterial community comprised majority or dominance of Ochrobactrum sp. following Cupriavidus and uncultured bacteria strains in paddy soil of selected sites. Initially, we have analyzed the abundance of the nitrous oxide reductase gene (nosZ), which was found to be related with PLFA based lipid profile. Chandauli of Eastern UP, India represented greater amount of lipid content (C18-C20) and denitrifier’s diversity. This study suggests the positive co-relation between soil PLFA profiles, DGGE, and Q-PCR data. Thus, a close networking among metabolic abilities and taxonomic composition of soil microbial communities existed, and subsequently, such work at greater extent could be helpful in managing nutrient dynamics as well as microbial dynamics of paddy soil ecosystem. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=denaturing%20gradient%20gel%20electrophoresis" title="denaturing gradient gel electrophoresis">denaturing gradient gel electrophoresis</a>, <a href="https://publications.waset.org/abstracts/search?q=DGGE" title=" DGGE"> DGGE</a>, <a href="https://publications.waset.org/abstracts/search?q=nitrifying%20and%20denitrifying%20bacteria" title=" nitrifying and denitrifying bacteria"> nitrifying and denitrifying bacteria</a>, <a href="https://publications.waset.org/abstracts/search?q=PLFA" title=" PLFA"> PLFA</a>, <a href="https://publications.waset.org/abstracts/search?q=Q-PCR" title=" Q-PCR"> Q-PCR</a> </p> <a href="https://publications.waset.org/abstracts/111346/an-insight-into-the-paddy-soil-denitrifying-bacteria-and-their-relation-with-soil-phospholipid-fatty-acid-profile" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/111346.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">124</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">60</span> An Initial Evaluation of Newly Proposed Biomarker of Zinc Status in Humans: The Erythrocyte Linoleic Acid: Dihomo-γ-Linolenic Acid (LA:DGLA) Ratio</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Marija%20Knez">Marija Knez</a>, <a href="https://publications.waset.org/abstracts/search?q=James%20C.R.%20Stangoulis"> James C.R. Stangoulis</a>, <a href="https://publications.waset.org/abstracts/search?q=Manja%20Zec"> Manja Zec</a>, <a href="https://publications.waset.org/abstracts/search?q=Zoran%20Pavlovic"> Zoran Pavlovic</a>, <a href="https://publications.waset.org/abstracts/search?q=Jasmina%20D.%20Martacic"> Jasmina D. Martacic</a>, <a href="https://publications.waset.org/abstracts/search?q=Mirjana%20Gurinovic"> Mirjana Gurinovic</a>, <a href="https://publications.waset.org/abstracts/search?q=Maria%20Glibetic"> Maria Glibetic</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Background: Zinc is an essential micronutrient for humans with important physiological functions. A sensitive and specific biomarker for assessing Zn status is still needed. Objective: The major aim of this study was to examine if the changes in the content of plasma phospholipid LA, DGLA and LA: DGLA ratio can be used to efficiently predict the dietary Zn intake and plasma Zn status of humans. Methods: The study was performed on apparently healthy human volunteers. The dietary Zn intake was assessed using 24h recall questionnaires. Plasma phospholipid fatty acid analysis was done by gas chromatography and plasma analysis of minerals by atomic absorption spectrometry. Biochemical, anthropometrical and hematological parameters were assessed. Results: No significant relationship was found between the dietary and plasma zinc status (r=0.07; p=0.6). There is a statistically significant correlation between DGLA and plasma Zn (r=0.39, p=0.00). No relationship was observed between the linoleic acid and plasma Zn, while there was a significant negative correlation between LA: DGLA ratio and plasma Zn status (r=-0.35, p=0.01). Similarly, there were statistically significant difference in DGLA status (p=0.004) and LA: DGLA ratio (p=0.042) between the Zn formed groups. Conclusions: This study is an initial step in evaluating LA: DGLA ratio as a biomarker of Zn status in humans. The results are encouraging as they show that concentration of DGLA is decreased and LA: DGLA ratio increased in people with lower dietary Zn intake. However, additional studies are needed to fully examine the sensitivity of this biomarker. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=dietary%20Zn%20intake%20Zinc" title="dietary Zn intake Zinc">dietary Zn intake Zinc</a>, <a href="https://publications.waset.org/abstracts/search?q=fatty%20acid%20composition" title=" fatty acid composition"> fatty acid composition</a>, <a href="https://publications.waset.org/abstracts/search?q=LA%3A%20DGLA" title=" LA: DGLA"> LA: DGLA</a>, <a href="https://publications.waset.org/abstracts/search?q=healthy%20population" title=" healthy population"> healthy population</a>, <a href="https://publications.waset.org/abstracts/search?q=plasma%20Zn%20status" title=" plasma Zn status"> plasma Zn status</a>, <a href="https://publications.waset.org/abstracts/search?q=Zn%20biomarker" title=" Zn biomarker"> Zn biomarker</a> </p> <a href="https://publications.waset.org/abstracts/45975/an-initial-evaluation-of-newly-proposed-biomarker-of-zinc-status-in-humans-the-erythrocyte-linoleic-acid-dihomo-gh-linolenic-acid-ladgla-ratio" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/45975.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">270</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">59</span> Preparation and Physicochemical Characterization of Non-ionic Surfactant Vesicles Containing Itraconazole </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Ataei">S. Ataei</a>, <a href="https://publications.waset.org/abstracts/search?q=F.%20Sarrafzadeh%20Javadi"> F. Sarrafzadeh Javadi</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Gilani"> K. Gilani</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Moazeni"> E. Moazeni</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Drug delivery systems using colloidal particulate carriers such as niosomes or liposomes have distinct advantages over conventional dosage forms because the particles can act as drug-containing reservoirs. These carriers play an increasingly important role in drug delivery. Niosomes are vesicular delivery systems which result from the self-assembly of hydrated surfactant. Niosomes are now widely studied as an attractive to liposomes because they alleviate the disadvantages associated with liposomes, such as chemical instability, variable purity of phospholipids and high cost. The encapsulation of drugs in niosomes can decrease drug toxicity, increase the stability of drug and increase the penetrability of drug in the location of application, and may reduce the dose and systemic side effect. Nowadays, Niosomes are used by the pharmaceutical industry in manufacturing skin medications, eye medication, in cosmetic formulas and these vesicular systems can be used to deliver aspiratory drugs. One way of improving dispersion in the water phase and solubility of the hydrophobic drug is to formulate in into niosomes. Itraconazole (ITZ) was chosen as a model hydrophobic drug. This drug is water insoluble (solubility ~ 1 ng/ml at neutral pH), is a broad-spectrum triazole antifungal agent and is used to treat various fungal disease. This study aims to investigate the capability of forming itraconazole niosomes with Spans, Tweens, Brijs as non-ionic surfactants. To this end, various formulations of niosomes have been studied with regard to parameters such as the degree of containment and particle size. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=physicochemical" title="physicochemical">physicochemical</a>, <a href="https://publications.waset.org/abstracts/search?q=non-ionic%20surfactant%20vesicles" title=" non-ionic surfactant vesicles"> non-ionic surfactant vesicles</a>, <a href="https://publications.waset.org/abstracts/search?q=itraconazole" title=" itraconazole"> itraconazole</a> </p> <a href="https://publications.waset.org/abstracts/18011/preparation-and-physicochemical-characterization-of-non-ionic-surfactant-vesicles-containing-itraconazole" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/18011.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">462</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">58</span> Artificial Cells Capable of Communication by Using Polymer Hydrogel</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Qi%20Liu">Qi Liu</a>, <a href="https://publications.waset.org/abstracts/search?q=Jiqin%20Yao"> Jiqin Yao</a>, <a href="https://publications.waset.org/abstracts/search?q=Xiaohu%20Zhou"> Xiaohu Zhou</a>, <a href="https://publications.waset.org/abstracts/search?q=Bo%20Zheng"> Bo Zheng</a> </p> <p class="card-text"><strong>Abstract:</strong></p> The first artificial cell was produced by Thomas Chang in the 1950s when he was trying to make a mimic of red blood cells. Since then, many different types of artificial cells have been constructed from one of the two approaches: a so-called bottom-up approach, which aims to create a cell from scratch, and a top-down approach, in which genes are sequentially knocked out from organisms until only the minimal genome required for sustaining life remains. In this project, bottom-up approach was used to build a new cell-free expression system which mimics artificial cell that capable of protein expression and communicate with each other. The artificial cells constructed from the bottom-up approach are usually lipid vesicles, polymersomes, hydrogels or aqueous droplets containing the nucleic acids and transcription-translation machinery. However, lipid vesicles based artificial cells capable of communication present several issues in the cell communication research: (1) The lipid vesicles normally lose the important functions such as protein expression within a few hours. (2) The lipid membrane allows the permeation of only small molecules and limits the types of molecules that can be sensed and released to the surrounding environment for chemical communication; (3) The lipid vesicles are prone to rupture due to the imbalance of the osmotic pressure. To address these issues, the hydrogel-based artificial cells were constructed in this work. To construct the artificial cell, polyacrylamide hydrogel was functionalized with Acrylate PEG Succinimidyl Carboxymethyl Ester (ACLT-PEG2000-SCM) moiety on the polymer backbone. The proteinaceous factors can then be immobilized on the polymer backbone by the reaction between primary amines of proteins and N-hydroxysuccinimide esters (NHS esters) of ACLT-PEG2000-SCM, the plasmid template and ribosome were encapsulated inside the hydrogel particles. Because the artificial cell could continuously express protein with the supply of nutrients and energy, the artificial cell-artificial cell communication and artificial cell-natural cell communication could be achieved by combining the artificial cell vector with designed plasmids. The plasmids were designed referring to the quorum sensing (QS) system of bacteria, which largely relied on cognate acyl-homoserine lactone (AHL) / transcription pairs. In one communication pair, “sender” is the artificial cell or natural cell that can produce AHL signal molecule by synthesizing the corresponding signal synthase that catalyzed the conversion of S-adenosyl-L-methionine (SAM) into AHL, while the “receiver” is the artificial cell or natural cell that can sense the quorum sensing signaling molecule form “sender” and in turn express the gene of interest. In the experiment, GFP was first immobilized inside the hydrogel particle to prove that the functionalized hydrogel particles could be used for protein binding. After that, the successful communication between artificial cell-artificial cell and artificial cell-natural cell was demonstrated, the successful signal between artificial cell-artificial cell or artificial cell-natural cell could be observed by recording the fluorescence signal increase. The hydrogel-based artificial cell designed in this work can help to study the complex communication system in bacteria, it can also be further developed for therapeutic applications. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=artificial%20cell" title="artificial cell">artificial cell</a>, <a href="https://publications.waset.org/abstracts/search?q=cell-free%20system" title=" cell-free system"> cell-free system</a>, <a href="https://publications.waset.org/abstracts/search?q=gene%20circuit" title=" gene circuit"> gene circuit</a>, <a href="https://publications.waset.org/abstracts/search?q=synthetic%20biology" title=" synthetic biology"> synthetic biology</a> </p> <a href="https://publications.waset.org/abstracts/120873/artificial-cells-capable-of-communication-by-using-polymer-hydrogel" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/120873.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">152</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">57</span> Development of Agomelatine Loaded Proliposomal Powders for Improved Intestinal Permeation: Effect of Surface Charge</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rajasekhar%20Reddy%20Poonuru">Rajasekhar Reddy Poonuru</a>, <a href="https://publications.waset.org/abstracts/search?q=Anusha%20Parnem"> Anusha Parnem</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Purpose: To formulate proliposome powder of agomelatine, an antipsychotic drug, and to evaluate physicochemical, in vitro characters and effect of surface charge on ex vivo intestinal permeation. Methods: Film deposition technique was employed to develop proliposomal powders of agomelatin with varying molar ratios of lipid Hydro Soy PC L-α-phosphatidylcholine (HSPC) and cholesterol with fixed sum of drug. With the aim to derive free flowing and stable proliposome powder, fluid retention potential of various carriers was examined. Liposome formation and number of vesicles formed for per mm3 up on hydration, vesicle size, and entrapment efficiency was assessed to deduce an optimized formulation. Sodium cholate added to optimized formulation to induce surface charge on formed vesicles. Solid-state characterization (FTIR, DSC, and XRD) was performed with the intention to assess native crystalline and chemical behavior of drug. The in vitro dissolution test of optimized formulation along with pure drug was evaluated to estimate dissolution efficiency (DE) and relative dissolution rate (RDR). Effective permeability co-efficient (Peff(rat)) in rat and enhancement ratio (ER) of drug from formulation and pure drug dispersion were calculated from ex vivo permeation studies in rat ileum. Results: Proliposomal powder formulated with equimolar ratio of HSPC and cholesterol ensued in higher no. of vesicles (3.95) with 90% drug entrapment up on hydration. Neusilin UFL2 was elected as carrier because of its high fluid retention potential (4.5) and good flow properties. Proliposome powder exhibited augmentation in DE (60.3 ±3.34) and RDR (21.2±01.02) of agomelation over pure drug. Solid state characterization studies demonstrated the transformation of native crystalline form of drug to amorphous and/or molecular state, which was in correlation with results obtained from in vitro dissolution test. The elevated Peff(rat) of 46.5×10-4 cm/sec and ER of 2.65 of drug from charge induced proliposome formulation with respect to pure drug dispersion was assessed from ex vivo intestinal permeation studies executed in ileum of wistar rats. Conclusion: Improved physicochemical characters and ex vivo intestinal permeation of drug from charge induced proliposome powder with Neusilin UFL2 unravels the potentiality of this system in enhancing oral delivery of agomelatin. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=agomelatin" title="agomelatin">agomelatin</a>, <a href="https://publications.waset.org/abstracts/search?q=proliposome" title=" proliposome"> proliposome</a>, <a href="https://publications.waset.org/abstracts/search?q=sodium%20cholate" title=" sodium cholate"> sodium cholate</a>, <a href="https://publications.waset.org/abstracts/search?q=neusilin" title=" neusilin"> neusilin</a> </p> <a href="https://publications.waset.org/abstracts/139924/development-of-agomelatine-loaded-proliposomal-powders-for-improved-intestinal-permeation-effect-of-surface-charge" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/139924.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">136</span> </span> </div> </div> <ul class="pagination"> <li class="page-item disabled"><span class="page-link">&lsaquo;</span></li> <li class="page-item active"><span class="page-link">1</span></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=phospholipid%20vesicles&page=2">2</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=phospholipid%20vesicles&page=3">3</a></li> <li class="page-item"><a class="page-link" href="https://publications.waset.org/abstracts/search?q=phospholipid%20vesicles&page=2" rel="next">&rsaquo;</a></li> </ul> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" 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