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<!DOCTYPE html> <html lang="en"> <head> <meta charset="UTF-8" /> <meta property="og:title" content="TLS" /> <meta property="og:url" content="https://www.ddbj.nig.ac.jp/ddbj/tls-e.html" /> <meta property="og:description" content="Since 2016, INSDC has accepted sequence data including 16S rRNA or some other ta..." /> <meta property6="og:image" content="/images/thumbnail/logo_ddbj_fb.png" /> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <title>TLS</title> <script async src="https://www.google-analytics.com/analytics.js"></script> <script src="https://code.jquery.com/jquery-3.5.0.js" integrity="sha256-r/AaFHrszJtwpe+tHyNi/XCfMxYpbsRg2Uqn0x3s2zc=" crossorigin="anonymous"></script> <script src="https://cdnjs.cloudflare.com/ajax/libs/jquery.hoverintent/1.10.1/jquery.hoverIntent.min.js" integrity="sha512-gx3WTM6qxahpOC/hBNUvkdZARQ2ObXSp/m+jmsEN8ZNJPymj8/Jamf8+/3kJQY1RZA2DR+KQfT+b3JEB0r9YRg==" crossorigin="anonymous"></script> <script 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class="internal-link"> </nav> </div> <section id="MainContentView" class="mainview"> <header class="header"> <nav class="breadcrumb-view"> <ul> <li> <a href="https://www.ddbj.nig.ac.jp/index-e.html">Home</a> </li> <li> <a href="https://www.ddbj.nig.ac.jp/ddbj/index-e.html">ddbj</a> </li> <li><a>TLS</a></li> </ul> </nav> <h1 class="title">TLS</h1> </header> <main class="md-content"> <p>Since 2016, INSDC has accepted sequence data including 16S rRNA or some other targeted loci mainly to be clustered into operational taxonomic unit as Targeted Locus Study (TLS) data type.</p> <p>With regard to comprehensive analysis of marker sequences by using NGS, TLS data submission is not required in many cases, because it is reproducible if the original reads are submitted to <a href="/dra/index-e.html">DDBJ Read Archive</a>.<br /> So, please consider if you have to submit TLS data to DDBJ or not.</p> <p>See <a href="https://ddbj.nig.ac.jp/public/ddbj_database/tls/TLS_ORGANISM_LIST.html">the list of publicized TLS data</a>.</p> <p>You can submit TSA data to DDBJ through <a href="/ddbj/mss-e.html">Mass Submission System (MSS)</a>.</p> <dl> <dt>Notes on the TLS data submission</dt> <dd> <ul> <li>Prior to TLS data submission, it is required to submit to <a href="/bioproject/index-e.html">BioProject Database</a> and <a href="/biosample/index-e.html">BioSample Database</a>.</li> <li>It is strongly recommended that the TLS data submission with the original sequence data of primary sequences are classified into <a href="/dra/index-e.html">DDBJ Read Archive</a>.</li> <li>Remove low quality reads and chimeric sequences before submission.</li> </ul> </dd> </dl> <h2 id="flat-file">Sample flat file</h2> <p>Aspects of TLS</p> <ul> <li>Basically, each TLS sequence submitted to DDBJ is assigned an <a href="#AccessionA">accession number</a> that consists of 4 alphabet characters and 8 digits.</li> <li>“TLS:” is shown at the beginning of <a href="#DefinitionA">DEFINITION</a> line.</li> <li>“TLS” and “Targeted Locus Study” are indicated in <a href="#KeywordsA">KEYWORDS</a> line.</li> </ul> <!-- end list --> <pre><code><a id="LocusA" href="/ddbj/flat-file-e#LocusB">LOCUS</a> <a id="LocusNameA" href="/ddbj/flat-file-e#LocusNameB">TZZZ01000001</a> <a id="SequenceLengthA" href="/ddbj/flat-file-e#SequenceLengthB">800 bp</a> <a id="MoleculeTypeA" href="/ddbj/flat-file-e#MoleculeTypeB">mRNA</a> <a id="MoleculeFormA" href="/ddbj/flat-file-e#MoleculeFormB">linear</a> <a id="DivisionA" href="/ddbj/flat-file-e#DivisionB">TLS</a> <a id="ModificationDateA" href="/ddbj/flat-file-e#ModificationDateB">15-NOV-2017</a> <a id="DefinitionA" href="/ddbj/flat-file-e#DefinitionB">DEFINITION</a> TLS: Uncultured bacterium OTU:MS213 gene for 16S ribosomal RNA, partial sequence. <a id="AccessionA" href="/ddbj/flat-file-e#AccessionB">ACCESSION</a> TZZZ01000001 <a id="VersionA" href="/ddbj/flat-file-e#VersionB">VERSION</a> TZZZ01000001.1 <a id="DblinkA" href="/ddbj/flat-file-e#DblinkB">DBLINK</a> BioProject:PRJDA43211 Sequence Read Archive: DRR800001 BioSample: SAMD98765439 <a id="KeywordsA" href="/ddbj/flat-file-e#KeywordsB">KEYWORDS</a> TLS; Targeted Locus Study; ENV. <a id="SourceA" href="/ddbj/flat-file-e#SourceB">SOURCE</a> uncultured bacterium <a id="OrganismA" href="/ddbj/flat-file-e#OrganismB">ORGANISM</a> uncultured bacterium Bacteria; environmental samples. <a id="Reference1A" href="/ddbj/flat-file-e#Reference1B">REFERENCE 1</a> (bases 1 to 800) <a id="AuthorsA" href="/ddbj/flat-file-e#AuthorsB">AUTHORS</a> Mishima,H. and Shizuoka,T. <a id="TitleA" href="/ddbj/flat-file-e#TitleB">TITLE</a> Direct Submission <a id="JournalA" href="/ddbj/flat-file-e#JournalB">JOURNAL</a> Submitted (30-SEP-2017) to the DDBJ/EMBL/GenBank databases. Contact:Hanako Mishima National Institute of Genetics, DNA Data Bank of Japan; Yata 1111, Mishima, Shizuoka 411-8540, Japan <a id="Reference2A" href="/ddbj/flat-file-e#Reference2B">REFERENCE 2</a> AUTHORS Mishima,H., Shizuoka,T. and Fuji,I. TITLE Metagenomic Taxonomy Profile in Sea Water JOURNAL TLS Biol 15, 161-170 (2017) <a id="CommentA" href="/ddbj/flat-file-e#CommentB">COMMENT</a> ##Assembly-Data-START## Assembly Method :: FLASH v. 2015 Sequencing Technology :: Illumina ##Assembly-Data-END## <a id="FeaturesA" href="/ddbj/flat-file-e#FeaturesB">FEATURES</a> Location/Qualifiers <a href="/ddbj/features.html#source">source</a> <a href="/ddbj/location-e.html">1..301</a> /<a href="/ddbj/qualifiers-e.html#altitude">altitude</a>="-20 m" /<a href="/ddbj/qualifiers-e.html#collection_date">collection_date</a>="2007" /<a href="/ddbj/qualifiers-e.html#db_xref">db_xref</a>="taxon:77133" /<a href="/ddbj/qualifiers-e.html#environmental_sample">environmental_sample</a> /<a href="/ddbj/qualifiers-e.html#geo_loc_name">geo_loc_name</a>="Pacific Ocean" /<a href="/ddbj/qualifiers-e.html#isolation_source">isolation_source</a>="marine water" /<a href="/ddbj/qualifiers-e.html#lat_lon">lat_lon</a>="29.3116 N 148.6778 E" /<a href="/ddbj/qualifiers-e.html#mol_type">mol_type</a>="genomic DNA" /<a href="/ddbj/qualifiers-e.html#organism">organism</a>="uncultured bacterium" /<a href="/ddbj/qualifiers-e.html#submitter_seqid">submitter_seqid</a>="OTU:MS213" <a href="/ddbj/features.html#rRNA">rRNA</a> <a href="/ddbj/location-e.html"><1..>301</a> /<a href="/ddbj/qualifiers-e.html#product">product</a>="16S ribosomal RNA" <a id="BaseCountA" href="#BaseCountB">BASE COUNT</a> 75 a 59 c 102 g 65 t <a id="OriginA" href="#OriginB">ORIGIN</a> 1 cagtcgccgc gggaatacgg agggggctag cgttgttcgg aattactggg cgtaaagcgc : -- The rest of nucleotide sequence is omitted -- : <a id="EndA" href="/ddbj/flat-file-e#EndB">//</a></code></pre> </main> <aside class="related-pages"> <h2 class="caption">Related pages</h2> <div class="navigation"> <nav> <ul> <li> <a href="/ddbj/genome-e.html">Data Submission from Genome Project</a> </li> <li> <a href="/ddbj/environmental-e.html">Submission of environmental sequences</a> </li> <li> <a href="/ddbj/transcriptome-e.html">Data Submission from Transcriptome Project</a> </li> <li> <a href="/ddbj/tpa-e.html">Third Party Data (TPA)</a> </li> </ul> </nav> </div> </aside> </section> </div> </section> </div> <footer></footer> <div id="back-top"></div> </body> </html>