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aberrations</title> <meta name="description" content="Search results for: chromosomal aberrations"> <meta name="keywords" content="chromosomal aberrations"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block 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action="https://publications.waset.org/search"> <div id="custom-search-input"> <div class="input-group"> <i class="fas fa-search"></i> <input type="text" class="search-query" name="q" placeholder="Author, Title, Abstract, Keywords" value="chromosomal aberrations"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 9</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: chromosomal aberrations</h1> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> Assessment of Occupational Exposure and Individual Radio-Sensitivity in People Subjected to Ionizing Radiation </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Oksana%20G.%20Cherednichenko">Oksana G. Cherednichenko</a>, <a href="https://publications.waset.org/search?q=Anastasia%20L.%20Pilyugina"> Anastasia L. Pilyugina</a>, <a href="https://publications.waset.org/search?q=Sergey%20N.Lukashenko"> Sergey N.Lukashenko</a>, <a href="https://publications.waset.org/search?q=Elena%20G.%20Gubitskaya"> Elena G. Gubitskaya</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>The estimation of accumulated radiation doses in people professionally exposed to ionizing radiation was performed using methods of biological (chromosomal aberrations frequency in lymphocytes) and physical (radionuclides analysis in urine, whole-body radiation meter, individual thermoluminescent dosimeters) dosimetry. A group of 84 "A" category employees after their work in the territory of former Semipalatinsk test site (Kazakhstan) was investigated. The dose rate in some funnels exceeds 40 μSv/h. After radionuclides determination in urine using radiochemical and WBC methods, it was shown that the total effective dose of personnel internal exposure did not exceed 0.2 mSv/year, while an acceptable dose limit for staff is 20 mSv/year. The range of external radiation doses measured with individual thermo-luminescent dosimeters was 0.3-1.406 µSv. The cytogenetic examination showed that chromosomal aberrations frequency in staff was 4.27±0.22%, which is significantly higher than at the people from non-polluting settlement Tausugur (0.87±0.1%) (р ≤ 0.01) and citizens of Almaty (1.6±0.12%) (р≤ 0.01). Chromosomal type aberrations accounted for 2.32±0.16%, 0.27±0.06% of which were dicentrics and centric rings. The cytogenetic analysis of different types group radiosensitivity among «professionals» (age, sex, ethnic group, epidemiological data) revealed no significant differences between the compared values. Using various techniques by frequency of dicentrics and centric rings, the average cumulative radiation dose for group was calculated, and that was 0.084-0.143 Gy. To perform comparative individual dosimetry using physical and biological methods of dose assessment, calibration curves (including own ones) and regression equations based on general frequency of chromosomal aberrations obtained after irradiation of blood samples by gamma-radiation with the dose rate of 0,1 Gy/min were used. Herewith, on the assumption of individual variation of chromosomal aberrations frequency (1–10%), the accumulated dose of radiation varied 0-0.3 Gy. The main problem in the interpretation of individual dosimetry results is reduced to different reaction of the objects to irradiation - radiosensitivity, which dictates the need of quantitative definition of this individual reaction and its consideration in the calculation of the received radiation dose. The entire examined contingent was assigned to a group based on the received dose and detected cytogenetic aberrations. Radiosensitive individuals, at the lowest received dose in a year, showed the highest frequency of chromosomal aberrations (5.72%). In opposite, radioresistant individuals showed the lowest frequency of chromosomal aberrations (2.8%). The cohort correlation according to the criterion of radio-sensitivity in our research was distributed as follows: radio-sensitive (26.2%) — medium radio-sensitivity (57.1%), radioresistant (16.7%). Herewith, the dispersion for radioresistant individuals is 2.3; for the group with medium radio-sensitivity — 3.3; and for radio-sensitive group — 9. These data indicate the highest variation of characteristic (reactions to radiation effect) in the group of radio-sensitive individuals. People with medium radio-sensitivity show significant long-term correlation (0.66; n=48, β ≥ 0.999) between the values of doses defined according to the results of cytogenetic analysis and dose of external radiation obtained with the help of thermoluminescent dosimeters. Mathematical models based on the type of violation of the radiation dose according to the professionals radiosensitivity level were offered.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Biodosimetry" title="Biodosimetry">Biodosimetry</a>, <a href="https://publications.waset.org/search?q=chromosomal%20aberrations" title=" chromosomal aberrations"> chromosomal aberrations</a>, <a href="https://publications.waset.org/search?q=ionizing%20radiation" title=" ionizing radiation"> ionizing radiation</a>, <a href="https://publications.waset.org/search?q=radiosensitivity." title=" radiosensitivity. "> radiosensitivity. </a> </p> <a href="https://publications.waset.org/10007565/assessment-of-occupational-exposure-and-individual-radio-sensitivity-in-people-subjected-to-ionizing-radiation" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10007565/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10007565/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10007565/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10007565/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10007565/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10007565/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10007565/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10007565/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10007565/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10007565/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10007565.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">938</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Opto-Mechanical Characterization of Aspheric Lenses from the Hybrid Method</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Aliouane%20Toufik">Aliouane Toufik</a>, <a href="https://publications.waset.org/search?q=Hamdi%20Amine"> Hamdi Amine</a>, <a href="https://publications.waset.org/search?q=Bouzid%20Djamel"> Bouzid Djamel</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>Aspheric optical components are an alternative to the use of conventional lenses in the implementation of imaging systems for the visible range. Spherical lenses are capable of producing aberrations. Therefore, they are not able to focus all the light into a single point. Instead, aspherical lenses correct aberrations and provide better resolution even with compact lenses incorporating a small number of lenses.</p> <p>Metrology of these components is very difficult especially when the resolution requirements increase and insufficient or complexity of conventional tools requires the development of specific approaches to characterization.</p> <p>This work is part of the problem existed because the objectives are the study and comparison of different methods used to measure surface rays hybrid aspherical lenses.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Aspherical%20surface" title="Aspherical surface">Aspherical surface</a>, <a href="https://publications.waset.org/search?q=Manufacture%20of%20lenses" title=" Manufacture of lenses"> Manufacture of lenses</a>, <a href="https://publications.waset.org/search?q=precision%20molding" title=" precision molding"> precision molding</a>, <a href="https://publications.waset.org/search?q=radius%20of%20curvature" title=" radius of curvature"> radius of curvature</a>, <a href="https://publications.waset.org/search?q=roughness." title=" roughness. "> roughness. </a> </p> <a href="https://publications.waset.org/9998915/opto-mechanical-characterization-of-aspheric-lenses-from-the-hybrid-method" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/9998915/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/9998915/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/9998915/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/9998915/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/9998915/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/9998915/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/9998915/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/9998915/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/9998915/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/9998915/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/9998915.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">2015</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> Evolutionary Distance in the Yeast Genome</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Somayyeh%20Azizi">Somayyeh Azizi</a>, <a href="https://publications.waset.org/search?q=Saeed%20Kaboli"> Saeed Kaboli</a>, <a href="https://publications.waset.org/search?q=Atsushi%20Yagi"> Atsushi Yagi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Whole genome duplication (WGD) increased the number of yeast Saccharomyces cerevisiae chromosomes from 8 to 16. In spite of retention the number of chromosomes in the genome of this organism after WGD to date, chromosomal rearrangement events have caused an evolutionary distance between current genome and its ancestor. Studies under evolutionary-based approaches on eukaryotic genomes have shown that the rearrangement distance is an approximable problem. In the case of S. cerevisiae, we describe that rearrangement distance is accessible by using dedoubled adjacency graph drawn for 55 large paired chromosomal regions originated from WGD. Then, we provide a program extracted from a C program database to draw a dedoubled genome adjacency graph for S. cerevisiae. From a bioinformatical perspective, using the duplicated blocks of current genome in S. cerevisiae, we infer that genomic organization of eukaryotes has the potential to provide valuable detailed information about their ancestrygenome. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Whole-genome%20duplication" title="Whole-genome duplication">Whole-genome duplication</a>, <a href="https://publications.waset.org/search?q=Evolution" title=" Evolution"> Evolution</a>, <a href="https://publications.waset.org/search?q=Double-cutand-%0Ajoin%20operation" title=" Double-cutand- join operation"> Double-cutand- join operation</a>, <a href="https://publications.waset.org/search?q=Yeast." title=" Yeast."> Yeast.</a> </p> <a href="https://publications.waset.org/3540/evolutionary-distance-in-the-yeast-genome" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/3540/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/3540/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/3540/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/3540/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/3540/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/3540/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/3540/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/3540/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/3540/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/3540/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/3540.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1505</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> A Study on Physicochemical Analysis of Road and Railway Track Side Soil Samples of Amritsar (Punjab) and Their Genotoxic Effects </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=R.%20Kaur">R. Kaur</a>, <a href="https://publications.waset.org/search?q=Y.%20B.%20Pakade"> Y. B. Pakade</a>, <a href="https://publications.waset.org/search?q=J.%20K.%20Katnoria"> J. K. Katnoria</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>Considering the serious health hazards of air pollutants from automobiles, the present study was aimed to estimate the genotoxic/tumor inducing potential of three soil samples collected from junctions of Bus stand (BS), Crystal (CT) and Railway station (RS) of Amritsar, Punjab (India) using <em>Allium cepa</em> root chromosomal aberration assay (<em>Al</em>RCAA) and potato disc tumor assay (PDTA). The genotoxic potential in <em>Al</em>RCAA was 41.27% and 41.26% for BS; 37.89% and 43.38% for RS and 33.76% and 37.83% for CT during <em>in situ</em> and root dip treatments, respectively. The maximum number of tumors were induced in RS sample (64) followed by BS (21) and CT (9) during PDTA. The physicochemical parameters of soil sample were also studied and the concentration of lead was found to be 95.21 mg/Kg in RS, 35.30 mg/Kg in BS and 24.59 mg/Kg in CT samples.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Automobiles" title="Automobiles">Automobiles</a>, <a href="https://publications.waset.org/search?q=genotoxicity" title=" genotoxicity"> genotoxicity</a>, <a href="https://publications.waset.org/search?q=Physicochemical%20parameters" title=" Physicochemical parameters"> Physicochemical parameters</a>, <a href="https://publications.waset.org/search?q=pollutants." title=" pollutants."> pollutants.</a> </p> <a href="https://publications.waset.org/9999202/a-study-on-physicochemical-analysis-of-road-and-railway-track-side-soil-samples-of-amritsar-punjab-and-their-genotoxic-effects" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/9999202/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/9999202/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/9999202/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/9999202/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/9999202/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/9999202/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/9999202/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/9999202/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/9999202/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/9999202/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/9999202.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">2228</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> A Pairwise-Gaussian-Merging Approach: Towards Genome Segmentation for Copy Number Analysis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Chih-Hao%20Chen">Chih-Hao Chen</a>, <a href="https://publications.waset.org/search?q=Hsing-Chung%20Lee"> Hsing-Chung Lee</a>, <a href="https://publications.waset.org/search?q=Qingdong%20Ling"> Qingdong Ling</a>, <a href="https://publications.waset.org/search?q=Hsiao-Jung%20Chen"> Hsiao-Jung Chen</a>, <a href="https://publications.waset.org/search?q=Sun-Chong%20Wang"> Sun-Chong Wang</a>, <a href="https://publications.waset.org/search?q=Li-Ching%20Wu"> Li-Ching Wu</a>, <a href="https://publications.waset.org/search?q=H.C.%20Lee"> H.C. Lee</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Segmentation, filtering out of measurement errors and identification of breakpoints are integral parts of any analysis of microarray data for the detection of copy number variation (CNV). Existing algorithms designed for these tasks have had some successes in the past, but they tend to be O(N2) in either computation time or memory requirement, or both, and the rapid advance of microarray resolution has practically rendered such algorithms useless. Here we propose an algorithm, SAD, that is much faster and much less thirsty for memory – O(N) in both computation time and memory requirement -- and offers higher accuracy. The two key ingredients of SAD are the fundamental assumption in statistics that measurement errors are normally distributed and the mathematical relation that the product of two Gaussians is another Gaussian (function). We have produced a computer program for analyzing CNV based on SAD. In addition to being fast and small it offers two important features: quantitative statistics for predictions and, with only two user-decided parameters, ease of use. Its speed shows little dependence on genomic profile. Running on an average modern computer, it completes CNV analyses for a 262 thousand-probe array in ~1 second and a 1.8 million-probe array in 9 seconds <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Cancer" title="Cancer">Cancer</a>, <a href="https://publications.waset.org/search?q=pathogenesis" title=" pathogenesis"> pathogenesis</a>, <a href="https://publications.waset.org/search?q=chromosomal%20aberration" title=" chromosomal aberration"> chromosomal aberration</a>, <a href="https://publications.waset.org/search?q=copy%0D%0Anumber%20variation" title=" copy number variation"> copy number variation</a>, <a href="https://publications.waset.org/search?q=segmentation%20analysis." title=" segmentation analysis."> segmentation analysis.</a> </p> <a href="https://publications.waset.org/14262/a-pairwise-gaussian-merging-approach-towards-genome-segmentation-for-copy-number-analysis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/14262/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/14262/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/14262/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/14262/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/14262/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/14262/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/14262/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/14262/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/14262/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/14262/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/14262.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1477</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Statistics of Exon Lengths in Animals, Plants, Fungi, and Protists</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Alexander%20Kaplunovsky">Alexander Kaplunovsky</a>, <a href="https://publications.waset.org/search?q=Vladimir%20Khailenko"> Vladimir Khailenko</a>, <a href="https://publications.waset.org/search?q=Alexander%20Bolshoy"> Alexander Bolshoy</a>, <a href="https://publications.waset.org/search?q=Shara%20Atambayeva"> Shara Atambayeva</a>, <a href="https://publications.waset.org/search?q=AnatoliyIvashchenko"> AnatoliyIvashchenko</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>Eukaryotic protein-coding genes are interrupted by spliceosomal introns, which are removed from the RNA transcripts before translation into a protein. The exon-intron structures of different eukaryotic species are quite different from each other, and the evolution of such structures raises many questions. We try to address some of these questions using statistical analysis of whole genomes. We go through all the protein-coding genes in a genome and study correlations between the net length of all the exons in a gene, the number of the exons, and the average length of an exon. We also take average values of these features for each chromosome and study correlations between those averages on the chromosomal level. Our data show universal features of exon-intron structures common to animals, plants, and protists (specifically, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Cryptococcus neoformans, Homo sapiens, Mus musculus, Oryza sativa, and Plasmodium falciparum). We have verified linear correlation between the number of exons in a gene and the length of a protein coded by the gene, while the protein length increases in proportion to the number of exons. On the other hand, the average length of an exon always decreases with the number of exons. Finally, chromosome clustering based on average chromosome properties and parameters of linear regression between the number of exons in a gene and the net length of those exons demonstrates that these average chromosome properties are genome-specific features.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Comparative%20genomics" title="Comparative genomics">Comparative genomics</a>, <a href="https://publications.waset.org/search?q=exon-intron%20structure" title=" exon-intron structure"> exon-intron structure</a>, <a href="https://publications.waset.org/search?q=eukaryotic%20clustering" title=" eukaryotic clustering"> eukaryotic clustering</a>, <a href="https://publications.waset.org/search?q=linear%20regression." title=" linear regression."> linear regression.</a> </p> <a href="https://publications.waset.org/5596/statistics-of-exon-lengths-in-animals-plants-fungi-and-protists" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/5596/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/5596/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/5596/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/5596/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/5596/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/5596/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/5596/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/5596/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/5596/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/5596/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/5596.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">2573</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Analysis of Plasmids and Restriction Fragment Length Polymorphisms of Acinetobacter baumannii Isolated from Hospitals- AL Jouf Region- KSA</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Samy%20A.%20Selim">Samy A. Selim</a>, <a href="https://publications.waset.org/search?q=Nashwa%20I.%20Hagag"> Nashwa I. Hagag</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Abstract–The objectives of the current study are to determine the prevalence, etiological agents, drug susceptibility pattern and plasmid profile of Acinetobacter baumannii isolates from Hospital-Acquired Infections (HAI) at Community Hospital, Al Jouf Province, Saudi Arabia. A total of 1890 patients had developed infection during hospital admission and were included in the study. Among those who developed nosocomial infections, 15(9.4), 10(2.7) and 118 (12.7) had respiratory tract infection (RTI), blood stream infections (BSI) and urinary tract (UTI) respectively. A total of 268 bacterial isolates were isolated from nosocomial infection. S. aureus was reported in 23.5% for of the total isolates followed by Klebsiella pneumoniae (17.5%), E. coli (17.2%), P. aeruginosa (11.9%), coagulase negative staphylococcus (9%), A. baumannii (7.1%), Enterobacter spp. (3.4%), Citrobacter freundii (3%), Proteus mirabilis (2.6%), and Proteus vulgaris and Enterococcous faecalis (0.7%). Isolated organisms are multi-drug resistant, predominantly Gram-positive pathogens with a high incidence of methicillin-resistant S. aureus, extended spectrum beta lactamase and vancomycin resistant enterococci organisms. The RFLP (Fragment Length Polymorphisms) patterns of plasmid preparations from isolated A. baumannii isolates had altered RFLP patterns, possibly due to the presence of plasmid(s). Five A. baumannii isolates harbored plasmids all of which were not less than 2.71kbp in molecular weight. Hence, it showed that the gene coding for the isolates were located on the plasmid DNA while the remaining isolates which have no plasmid might showed gene coding for antibiotic resistance being located on chromosomal DNA. Nosocomial infections represent a current problem in Community Hospital, Al Jouf Province, Saudi Arabia. Problems associated with SSI include infection with multidrug resistant pathogens which are difficult to treat and are associated with increased mortality. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Hospital-Acquired%20Infections" title="Hospital-Acquired Infections">Hospital-Acquired Infections</a>, <a href="https://publications.waset.org/search?q=Acinetobacter%20baumannii" title=" Acinetobacter baumannii"> Acinetobacter baumannii</a>, <a href="https://publications.waset.org/search?q=antibiotic%20resistance" title=" antibiotic resistance"> antibiotic resistance</a>, <a href="https://publications.waset.org/search?q=plasmid%20profile" title=" plasmid profile"> plasmid profile</a>, <a href="https://publications.waset.org/search?q=RFLP%20patterns" title=" RFLP patterns"> RFLP patterns</a>, <a href="https://publications.waset.org/search?q=Al%20Jouf%20Province" title=" Al Jouf Province"> Al Jouf Province</a>, <a href="https://publications.waset.org/search?q=Saudi%20Arabia" title=" Saudi Arabia"> Saudi Arabia</a> </p> <a href="https://publications.waset.org/6437/analysis-of-plasmids-and-restriction-fragment-length-polymorphisms-of-acinetobacter-baumannii-isolated-from-hospitals-al-jouf-region-ksa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/6437/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/6437/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/6437/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/6437/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/6437/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/6437/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/6437/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/6437/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/6437/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/6437/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/6437.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">2117</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Evaluation of Azo Dye Toxicity Using Some Haematological and Histopathological Alterations in Fish Catla catla</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Barot%20Jagruti">Barot Jagruti</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>The textile industry plays a major role in the economy of India and on the other side of the coin it is the major source for water pollution. As azo dyes is the largest dye class they are extensively used in many fields such as textile industry, leather tanning industry, paper production, food, color photography, pharmaceuticals and medicine, cosmetic, hair colorings, wood staining, agricultural, biological and chemical research etc. In addition to these, they can have acute and/or chronic effects on organisms depending on their concentration and length of exposure when they discharged as effluent in the environment. The aim of this study was to assess the genotoxic and histotoxic potentials of environmentally relevant concentrations of C. I. Reactive Red 120 (RR 120) on Catla catla, important edible freshwater fingerlings. For this, healthy Catla catla fingerlings were procured from the Government Fish Farm and acclimatized in 100 L capacity and continuously aerated glass aquarium in laboratory for 15 days. According to APHA some physic-chemical parameters were measured and maintained such as temperature, pH, dissolve oxygen, alkalinity, total hardness. Water along with excreta had been changed every 24 hrs. All fingerlings were fed artificial food palates once a day @ body weight. After 15 days fingerlings were grouped in 5 (10 in each) and exposed to various concentrations of RR 120 (Control, 10, 20, 30 and 40 mg.l-1) and samples (peripheral blood and gills, kidney) were collected and analyzed at 96 hrs. All results were compared with the control. Micronuclei (MN), nuclear buds (NB), fragmented-apoptotic (FA) and bi-nucleated (BN) cells in blood smears and in tissues (gills and kidney cells) were observed. Prominent histopathological alterations were noticed in gills such as aneurism, hyperplasia, degenerated central axis, lifting of gill epithelium, curved secondary gill lamellae etc. Similarly kidney showed some detrimental changes like shrunken glomeruli with increased periglomerular space, degenerated renal tubules etc. Both haematological and histopathological changes clearly reveal the toxic potential of RR 120. This work concludes that water pollution assessment can be done by these two biomarkers which provide baseline to the further chromosomal or molecular work.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Catla%20catla" title="Catla catla">Catla catla</a>, <a href="https://publications.waset.org/search?q=genotoxicity" title=" genotoxicity"> genotoxicity</a>, <a href="https://publications.waset.org/search?q=histopathlogicalchanges" title=" histopathlogicalchanges"> histopathlogicalchanges</a>, <a href="https://publications.waset.org/search?q=RR%20120azo%20dye." title=" RR 120azo dye."> RR 120azo dye.</a> </p> <a href="https://publications.waset.org/10001206/evaluation-of-azo-dye-toxicity-using-some-haematological-and-histopathological-alterations-in-fish-catla-catla" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10001206/apa" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">APA</a> <a href="https://publications.waset.org/10001206/bibtex" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">BibTeX</a> <a href="https://publications.waset.org/10001206/chicago" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Chicago</a> <a href="https://publications.waset.org/10001206/endnote" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">EndNote</a> <a href="https://publications.waset.org/10001206/harvard" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">Harvard</a> <a href="https://publications.waset.org/10001206/json" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">JSON</a> <a href="https://publications.waset.org/10001206/mla" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">MLA</a> <a href="https://publications.waset.org/10001206/ris" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">RIS</a> <a href="https://publications.waset.org/10001206/xml" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10001206/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10001206.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">2814</span> </span> </div> </div> <div class="card publication-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> From Primer Generation to Chromosome Identification: A Primer Generation Genotyping Method for Bacterial Identification and Typing</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/search?q=Wisam%20H.%20Benamer">Wisam H. Benamer</a>, <a href="https://publications.waset.org/search?q=Ehab%20A.%20Elfallah"> Ehab A. Elfallah</a>, <a href="https://publications.waset.org/search?q=Mohamed%20A.%20Elshaari"> Mohamed A. Elshaari</a>, <a href="https://publications.waset.org/search?q=Farag%20A.%20Elshaari"> Farag A. Elshaari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> <p>A challenge for laboratories is to provide bacterial identification and antibiotic sensitivity results within a short time. Hence, advancement in the required technology is desirable to improve timing, accuracy and quality. Even with the current advances in methods used for both phenotypic and genotypic identification of bacteria the need is there to develop method(s) that enhance the outcome of bacteriology laboratories in accuracy and time. The hypothesis introduced here is based on the assumption that the chromosome of any bacteria contains unique sequences that can be used for its identification and typing. The outcome of a pilot study designed to test this hypothesis is reported in this manuscript. Methods: The complete chromosome sequences of several bacterial species were downloaded to use as search targets for unique sequences. Visual basic and SQL server (2014) were used to generate a complete set of 18-base long primers, a process started with reverse translation of randomly chosen 6 amino acids to limit the number of the generated primers. In addition, the software used to scan the downloaded chromosomes using the generated primers for similarities was designed, and the resulting hits were classified according to the number of similar chromosomal sequences, i.e., unique or otherwise. Results: All primers that had identical/similar sequences in the selected genome sequence(s) were classified according to the number of hits in the chromosomes search. Those that were identical to a single site on a single bacterial chromosome were referred to as unique. On the other hand, most generated primers sequences were identical to multiple sites on a single or multiple chromosomes. Following scanning, the generated primers were classified based on ability to differentiate between medically important bacterial and the initial results looks promising. Conclusion: A simple strategy that started by generating primers was introduced; the primers were used to screen bacterial genomes for match. Primer(s) that were uniquely identical to specific DNA sequence on a specific bacterial chromosome were selected. The identified unique sequence can be used in different molecular diagnostic techniques, possibly to identify bacteria. In addition, a single primer that can identify multiple sites in a single chromosome can be exploited for region or genome identification. Although genomes sequences draft of isolates of organism DNA enable high throughput primer design using alignment strategy, and this enhances diagnostic performance in comparison to traditional molecular assays. In this method the generated primers can be used to identify an organism before the draft sequence is completed. In addition, the generated primers can be used to build a bank for easy access of the primers that can be used to identify bacteria.</p> <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/search?q=Bacteria%20chromosome" title="Bacteria chromosome">Bacteria chromosome</a>, <a href="https://publications.waset.org/search?q=bacterial%20identification" title=" bacterial identification"> bacterial identification</a>, <a href="https://publications.waset.org/search?q=sequence" title=" sequence"> sequence</a>, <a href="https://publications.waset.org/search?q=primer%20generation." title=" primer generation."> primer generation.</a> </p> <a href="https://publications.waset.org/10005718/from-primer-generation-to-chromosome-identification-a-primer-generation-genotyping-method-for-bacterial-identification-and-typing" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/10005718/apa" target="_blank" rel="nofollow" class="btn 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rel="nofollow" class="btn btn-primary btn-sm">XML</a> <a href="https://publications.waset.org/10005718/iso690" target="_blank" rel="nofollow" class="btn btn-primary btn-sm">ISO 690</a> <a href="https://publications.waset.org/10005718.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">1046</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div 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