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<!DOCTYPE html> <html lang="en" class="no-js"> <head> <meta charset="UTF-8"> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="applicable-device" content="pc,mobile"> <meta name="viewport" content="width=device-width, initial-scale=1"> <meta name="robots" content="max-image-preview:large"> <meta name="access" content="Yes"> <meta name="360-site-verification" content="1268d79b5e96aecf3ff2a7dac04ad990" /> <title>Decline in condition of gorgonian octocorals on mesophotic reefs in the northern Gulf of Mexico: before and after the Deepwater Horizon oil spill | Coral Reefs</title> <meta name="twitter:site" content="@SpringerLink"/> <meta name="twitter:card" content="summary_large_image"/> <meta name="twitter:image:alt" content="Content cover image"/> <meta name="twitter:title" content="Decline in condition of gorgonian octocorals on mesophotic reefs in the northern Gulf of Mexico: before and after the Deepwater Horizon oil spill"/> <meta name="twitter:description" content="Coral Reefs - Hard-bottom ‘mesophotic’ reefs along the ‘40-fathom’ (73&nbsp;m) shelf edge in the northern Gulf of Mexico were investigated for potential effects of the..."/> <meta name="twitter:image" content="https://static-content.springer.com/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig1_HTML.gif"/> <meta name="journal_id" content="338"/> <meta name="dc.title" content="Decline in condition of gorgonian octocorals on mesophotic reefs in the northern Gulf of Mexico: before and after the Deepwater Horizon oil spill"/> <meta name="dc.source" content="Coral Reefs 2015 35:1"/> <meta name="dc.format" content="text/html"/> <meta name="dc.publisher" content="Springer"/> <meta name="dc.date" content="2015-10-20"/> <meta name="dc.type" content="Report"/> <meta name="dc.language" content="En"/> <meta name="dc.copyright" content="2015 The Author(s)"/> <meta name="dc.rights" content="2015 The Author(s)"/> <meta name="dc.rightsAgent" content="journalpermissions@springernature.com"/> <meta name="dc.description" content="Hard-bottom ‘mesophotic’ reefs along the ‘40-fathom’ (73&nbsp;m) shelf edge in the northern Gulf of Mexico were investigated for potential effects of the Deepwater Horizon (DWH) oil spill from the Macondo well in April 2010. Alabama Alps Reef, Roughtongue Reef, and Yellowtail Reef were near the well, situated 60–88&nbsp;m below floating oil discharged during the DWH spill for several weeks and subject to dispersant applications. In contrast, Coral Trees Reef and Madison Swanson South Reef were far from the DWH spill site and below the slick for less than a week or not at all, respectively. The reefs were surveyed by ROV in 2010, 2011, and 2014 and compared to similar surveys conducted one and two decades earlier. Large gorgonian octocorals were present at all sites in moderate abundance including Swiftia exserta, Hypnogorgia pendula, Thesea spp., and Placogorgia spp. The gorgonians were assessed for health and condition in a before-after-control-impact (BACI) research design using still images captured from ROV video transects. Injury was modeled as a categorical response to proximity and time using logistic regression. Condition of gorgonians at sites near Macondo well declined significantly post-spill. Before the spill, injury was observed for 4–9&nbsp;% of large gorgonians. After the spill, injury was observed in 38–50&nbsp;% of large gorgonians. Odds of injury for sites near Macondo were 10.8 times higher post-spill, but unchanged at far sites. The majority of marked injured colonies in 2011 declined further in condition by 2014. Marked healthy colonies generally remained healthy. Background stresses to corals, including fishing activity, fishing debris, and coral predation, were noted during surveys, but do not appear to account for the decline in condition at study sites near Macondo well."/> <meta name="prism.issn" content="1432-0975"/> <meta name="prism.publicationName" content="Coral Reefs"/> <meta name="prism.publicationDate" content="2015-10-20"/> <meta name="prism.volume" content="35"/> <meta name="prism.number" content="1"/> <meta name="prism.section" content="Report"/> <meta name="prism.startingPage" content="77"/> <meta name="prism.endingPage" content="90"/> <meta name="prism.copyright" content="2015 The Author(s)"/> <meta name="prism.rightsAgent" content="journalpermissions@springernature.com"/> <meta name="prism.url" content="https://link.springer.com/article/10.1007/s00338-015-1363-2"/> <meta name="prism.doi" content="doi:10.1007/s00338-015-1363-2"/> <meta name="citation_pdf_url" content="https://link.springer.com/content/pdf/10.1007/s00338-015-1363-2.pdf"/> <meta name="citation_fulltext_html_url" content="https://link.springer.com/article/10.1007/s00338-015-1363-2"/> <meta name="citation_journal_title" content="Coral Reefs"/> <meta name="citation_journal_abbrev" content="Coral Reefs"/> <meta name="citation_publisher" content="Springer Berlin Heidelberg"/> <meta name="citation_issn" content="1432-0975"/> <meta name="citation_title" content="Decline in condition of gorgonian octocorals on mesophotic reefs in the northern Gulf of Mexico: before and after the Deepwater Horizon oil spill"/> <meta name="citation_volume" content="35"/> <meta name="citation_issue" content="1"/> <meta name="citation_publication_date" content="2016/03"/> <meta name="citation_online_date" content="2015/10/20"/> <meta name="citation_firstpage" content="77"/> <meta name="citation_lastpage" content="90"/> <meta name="citation_article_type" content="Report"/> <meta name="citation_fulltext_world_readable" content=""/> <meta name="citation_language" content="en"/> <meta name="dc.identifier" content="doi:10.1007/s00338-015-1363-2"/> <meta name="DOI" content="10.1007/s00338-015-1363-2"/> <meta name="size" content="220321"/> <meta name="citation_doi" content="10.1007/s00338-015-1363-2"/> <meta name="citation_springer_api_url" content="http://api.springer.com/xmldata/jats?q=doi:10.1007/s00338-015-1363-2&api_key="/> <meta name="description" content="Hard-bottom ‘mesophotic’ reefs along the ‘40-fathom’ (73&nbsp;m) shelf edge in the northern Gulf of Mexico were investigated fo"/> <meta name="dc.creator" content="Etnoyer, Peter J."/> <meta name="dc.creator" content="Wickes, Leslie N."/> <meta name="dc.creator" content="Silva, Mauricio"/> <meta name="dc.creator" content="Dubick, J. D."/> <meta name="dc.creator" content="Balthis, Len"/> <meta name="dc.creator" content="Salgado, Enrique"/> <meta name="dc.creator" content="MacDonald, Ian R."/> <meta name="dc.subject" content="Freshwater & Marine Ecology"/> <meta name="dc.subject" content="Oceanography"/> <meta name="citation_reference" content="citation_journal_title=Ecotoxicology; citation_title=Ingestion and sublethal effects of physically and chemically dispersed crude oil on marine planktonic copepods; citation_author=R Almeda, S Baca, C Hyatt, EJ Buskey; citation_volume=23; citation_publication_date=2014; citation_pages=988-1003; citation_doi=10.1007/s10646-014-1242-6; citation_id=CR1"/> <meta name="citation_reference" content="citation_title=The shallow-water Octocorallia of the West Indian region: a manual for marine biologists; citation_publication_date=1961; citation_id=CR2; citation_author=FM Bayer; citation_publisher=Martinus Nijhoff"/> <meta name="citation_reference" content="citation_journal_title=Proc Biol Soc Wash; citation_title=Key to the genera of Octocorallia exclusive of Pennatulacea (Coelenterata: Anthozoa), with diagnoses of new taxa; citation_author=FM Bayer; citation_volume=94; citation_publication_date=1981; citation_pages=902-947; citation_id=CR3"/> <meta name="citation_reference" content="citation_title=Mississippi-Alabama continental shelf ecosystem study: data summary and synthesis. OCS Study MMS 91-0062; citation_publication_date=1991; citation_id=CR4; citation_publisher=U.S. Department of the Interior, Minerals Management Service, Gulf of Mexico OCS Regional Office"/> <meta name="citation_reference" content="citation_title=Distribution and diversity of species associated with deep-sea gorgonian corals off Atlantic Canada; citation_inbook_title=Cold-water corals and ecosystems; citation_publication_date=2005; citation_pages=849-879; citation_id=CR5; citation_author=L Buhl-Mortensen; citation_author=PB Mortensen; citation_publisher=Springer"/> <meta name="citation_reference" content="citation_title=Octocorallia (Cnidaria) of the Gulf of Mexico; citation_inbook_title=Gulf of Mexico–origins, waters, and biota; citation_publication_date=2009; citation_pages=321-331; citation_id=CR6; citation_author=SD Cairns; citation_author=FM Bayer; citation_publisher=Texas A&M Press"/> <meta name="citation_reference" content="citation_journal_title=Environ Sci Technol; citation_title=Using natural abundance radiocarbon to trace the flux of petrocarbon to the seafloor following the Deepwater Horizon oil spill; citation_author=J Chanton, T Zhao, BE Rosenheim, S Joye, S Bosman, C Brunner, KM Yeager, AR Diercks, D Hollander; citation_volume=49; citation_publication_date=2014; citation_pages=847-854; citation_doi=10.1021/es5046524; citation_id=CR7"/> <meta name="citation_reference" content="citation_journal_title=Mar Ecol Prog Ser; citation_title=Statistical design and analysis for a ‘biological effects’ study; citation_author=KR Clarke, RH Green; citation_volume=46; citation_publication_date=1988; citation_pages=213-226; citation_doi=10.3354/meps046213; citation_id=CR8"/> <meta name="citation_reference" content="citation_title=PRIMER v6: user manual/tutorial; citation_publication_date=2006; citation_id=CR9; citation_author=KR Clarke; citation_author=RN Gorley; citation_publisher=PRIMER-E"/> <meta name="citation_reference" content="citation_journal_title=Environ Pollut; citation_title=Effects of Iranian crude oil on the Red Sea octocoral Heteroxenia fuscescens ; citation_author=Y Cohen, A Nissenbaum, R Eisler; citation_volume=12; citation_publication_date=1977; citation_pages=173-186; citation_doi=10.1016/0013-9327(77)90051-9; citation_id=CR10"/> <meta name="citation_reference" content="citation_journal_title=Mar Ecol Prog Ser; citation_title=Feeding and prey capture cycles in the aposymbiontic gorgonian Paramuricea clavata ; citation_author=R Coma, JM Gili, M Zabala, T Riera; citation_volume=115; citation_publication_date=1994; citation_pages=257-270; citation_doi=10.3354/meps115257; citation_id=CR11"/> <meta name="citation_reference" content="citation_title=Ecological condition of coastal waters along the U.S. Continental Shelf of the Northeastern Gulf of Mexico: 2010. NOAA Technical Memorandum NOS NCCOS 188; citation_publication_date=2014; citation_id=CR12; citation_author=C Cooksey; citation_author=J Hyland; citation_author=M Fulton; citation_author=L Balthis; citation_author=E Wirth; citation_author=T Wade; citation_publisher=NOAA National Ocean Service"/> <meta name="citation_reference" content="citation_journal_title=Limnol Oceanogr; citation_title=Downwelling and deep-water bottom currents as food supply mechanisms to the cold-water coral Lophelia pertusa (Scleractinia) at the Mingulay Reef complex; citation_author=AJ Davies, GC Duineveld, MS Lavaleye, MJ Bergman, H Haren, JM Roberts; citation_volume=54; citation_publication_date=2009; citation_pages=620-629; citation_doi=10.4319/lo.2009.54.2.0620; citation_id=CR13"/> <meta name="citation_reference" content="citation_journal_title=Deep Sea Res Part II Top Stud Oceanogr; citation_title=Response of deep-water corals to oil and chemical dispersant exposure; citation_author=DM DeLeo, DV Ruiz-Ramos, IB Baums, EE Cordes; citation_publication_date=2015; citation_id=CR14"/> <meta name="citation_reference" content="citation_journal_title=PLoS One; citation_title=Caribbean corals in crisis: record thermal stress, bleaching, and mortality in 2005; citation_author=CM Eakin, JA Morgan, SF Heron, TB Smith, G Liu, L Alvarez- Filip, B Baca, E Bartels, C Bastidas, C Bouchon, M Brandt, AW Bruckner, L Bunkley-Williams, A Cameron, B Causey, M Chiappone, TRL Christensen, MJC Crabbe, O Day, E Guardia, G Díaz-Pulido, D DiResta, D Gil-Agudelo, DS Gilliam, RN Ginsburg, S Gore, HM Guzmán, JC Hendee, EA Hernández-Delgado, E Husain, CFG Jeffrey, RJ Jones, E Jordán-Dahlgren, LS Kaufman, DI Kline, PA Kramer, JC Lang, D Lirman, J Mallela, C Manfrino, JP Maréchal, K Marks, J Mihaly, WJ Miller, EM Mueller, CA Orozco Toro, HA Oxenford, D Ponce-Taylor, N Quinn, KB Ritchie, S Rodríguez, A Rodríguez Ramírez, S Romano, JF Samhouri, JA Sánchez, GP Schmahl, BV Shank, WJ Skirving, SCC Steiner, E Villamizar, SM Walsh, C Walter, E Weil, EH Williams, K Woody Roberson, Y Yusuf; citation_volume=5; citation_issue=11; citation_publication_date=2010; citation_pages=e13969; citation_doi=10.1371/journal.pone.0013969; citation_id=CR15"/> <meta name="citation_reference" content="citation_title=Deep-sea coral collection protocols; citation_publication_date=2006; citation_id=CR16; citation_author=P Etnoyer; citation_author=SD Cairns; citation_author=JA Sanchez; citation_author=JK Reed; citation_author=JV Lopez; citation_author=WW Schroeder; citation_author=SD Brooke; citation_author=L Watling; citation_author=A Baco-Taylor; citation_author=GC Williams; citation_author=A Lindner; citation_author=SC France; citation_author=AW Bruckner; citation_publisher=NOAA Technical Memorandum NMFS-OPR-28"/> <meta name="citation_reference" content="citation_journal_title=Limnol Oceanogr; citation_title=Flow-dependent herbivory and growth in zooxanthellae-free soft corals; citation_author=KE Fabricius, A Genin, Y Benayahu; citation_volume=40; citation_publication_date=1995; citation_pages=1290-1301; citation_doi=10.4319/lo.1995.40.7.1290; citation_id=CR17"/> <meta name="citation_reference" content="citation_journal_title=BioScience; citation_title=Seaweeds and decapod crustaceans on Gulf deep banks after the Macondo oil spill; citation_author=DL Felder, BP Thoma, WE Schmidt, T Sauvage, SL Self-Krayesky, A Chistoserdov, HD Bracken-Grissom, S Fredericq; citation_volume=64; citation_publication_date=2014; citation_pages=808-819; citation_doi=10.1093/biosci/biu119; citation_id=CR18"/> <meta name="citation_reference" content="citation_journal_title=Bioscience; citation_title=Coral communities as indicators of ecosystem-level impacts of the deepwater horizon spill; citation_author=CR Fisher, AWJ Demopoulos, EE Cordes, IB Baums, HK White, JR Bourque; citation_volume=64; citation_publication_date=2014; citation_pages=796-807; citation_doi=10.1093/biosci/biu129; citation_id=CR19"/> <meta name="citation_reference" content="Gardner JV, Sulak KJ, Dartnell P, Hellequin L, Calder B, Mayer LA (2000) Cruise report RV Ocean Surveyor Cruise O-1-00-GM the bathymetry and acoustic backscatter of the Pinnacles Area, Northern Gulf of Mexico May 23, through June 10, 2000. Venice, LA to Venice, LA. U.S. Geological Survey Open-File Report 00-350"/> <meta name="citation_reference" content="citation_journal_title=Bull Mar Sci; citation_title=Invertebrate assemblages and ecological controls on topographic features in the Northeast Gulf of Mexico; citation_author=SR Gittings, TJ Bright, WW Schroeder, WW Sager, SJ Laswell, R Rezak; citation_volume=50; citation_publication_date=1992; citation_pages=435-455; citation_id=CR21"/> <meta name="citation_reference" content="citation_journal_title=PLoS One; citation_title=Toxicity of deepwater horizon source oil and the chemical dispersant, Corexit® 9500, to coral larvae; citation_author=G Goodbody-Gringley, DL Wetzel, D Gillon, E Pulster, A Miller, KB Ritchie; citation_volume=8; citation_publication_date=2013; citation_pages=e45574; citation_doi=10.1371/journal.pone.0045574; citation_id=CR22"/> <meta name="citation_reference" content="citation_journal_title=Environ Res Lett; citation_title=Oil carbon entered the coastal planktonic food web during the Deepwater Horizon oil spill; citation_author=WM Graham, RH Condon, RH Carmichael, I D’Ambra, HK Patterson, LJ Linn, FJ Hernandez; citation_volume=5; citation_publication_date=2010; citation_pages=e045301; citation_doi=10.1088/1748-9326/5/4/045301; citation_id=CR23"/> <meta name="citation_reference" content="citation_journal_title=Dis Aquat Org; citation_title=Diseases affect cold-water corals too: Eunicella verrucosa (Cnidaria: Gorgonacea) necrosis in SW England; citation_author=JM Hall-Spencer, J Pike, CB Munn; citation_volume=76; citation_publication_date=2007; citation_pages=87-97; citation_doi=10.3354/dao076087; citation_id=CR24"/> <meta name="citation_reference" content="citation_journal_title=Elem Sci Anthr; citation_title=Evidence of lasting impact of the Deepwater Horizon oil spill on a deep Gulf of Mexico coral community; citation_author=P-Y Hsing, B Fu, EA Larcom, SP Berlet, TM Shank, AF Govindarajan, AJ Lukasiewicz, PM Dixon, CR Fisher; citation_volume=1; citation_publication_date=2013; citation_pages=0000012; citation_doi=10.12952/journal.elementa.000012; citation_id=CR25"/> <meta name="citation_reference" content="citation_title=Pollution and degradation of coral reef communities; citation_inbook_title=Tropical marine pollution; citation_publication_date=1975; citation_pages=13-51; citation_id=CR26; citation_author=RE Johannes; citation_publisher=Elsevier"/> <meta name="citation_reference" content="citation_journal_title=Coral Reefs; citation_title=Community ecology of mesophotic coral reef systems; citation_author=SE Kahng, JR Garcia-Sais, HL Spalding, E Brokovich, D Wagner, E Weil, L Hinderstein, RJ Toonen; citation_volume=29; citation_publication_date=2010; citation_pages=255-275; citation_doi=10.1007/s00338-010-0593-6; citation_id=CR27"/> <meta name="citation_reference" content="citation_journal_title=Am Nat; citation_title=The rise and fall of a six year coral fungal epizootic; citation_author=K Kim, D Harvell; citation_volume=164; citation_issue=S5; citation_publication_date=2004; citation_pages=S52-S63; citation_doi=10.1086/424609; citation_id=CR28"/> <meta name="citation_reference" content="citation_journal_title=Hydrobiologia; citation_title=Megafauna associations with deepwater corals (Primnoa spp.) in the Gulf of Alaska; citation_author=KJ Krieger, BL Wing; citation_volume=471; citation_publication_date=2002; citation_pages=83-90; citation_doi=10.1023/A:1016597119297; citation_id=CR29"/> <meta name="citation_reference" content="citation_journal_title=Mar Environ Res; citation_title=Metamorphosis of Heteroxenia fuscescens planulae (Cnidaria: Octocorallia) is inhibited by crude oil: a novel short term toxicity bioassay; citation_author=A Kushmora, G Henning, DK Hofmann, Y Benayahu; citation_volume=43; citation_publication_date=1997; citation_pages=295-302; citation_doi=10.1016/S0141-1136(96)00092-X; citation_id=CR30"/> <meta name="citation_reference" content="citation_journal_title=Mar Ecol Prog Ser; citation_title=Effects of oil pollution on coral reef communities; citation_author=YM Loya, B Rinkevich; citation_volume=3; citation_publication_date=1980; citation_pages=167-180; citation_doi=10.3354/meps003167; citation_id=CR31"/> <meta name="citation_reference" content="citation_journal_title=Zool J Linn Soc; citation_title=Phylogenetic revision of the Hippasterinae (Goniasteridae; Asteroidea): systematics of deep sea corallivores, including one new genus and three new species; citation_author=C Mah, M Nizinski, L Lundsten; citation_volume=160; citation_publication_date=2010; citation_pages=266-301; citation_doi=10.1111/j.1096-3642.2010.00638.x; citation_id=CR32"/> <meta name="citation_reference" content="citation_journal_title=Proc Natl Acad Sci USA; citation_title=Review of flow rate estimates of the Deepwater Horizon oil spill; citation_author=MK McNutt, R Camilli, TJ Crone, GD Guthrie, PA Hsieh, TB Ryerson, O Savas, F Shaffer; citation_volume=109; citation_publication_date=2011; citation_pages=20260-20267; citation_doi=10.1073/pnas.1112139108; citation_id=CR33"/> <meta name="citation_reference" content="citation_journal_title=PLoS One; citation_title=Deep-sea benthic footprint of the Deepwater Horizon blowout; citation_author=PA Montagna, JG Baguley, C Cooksey, I Hartwell, LJ Hyde, JL Hyland, RD Kalke, LM Kracker, M Reuscher, ACE Rhodes; citation_volume=8; citation_publication_date=2013; citation_pages=e70540; citation_doi=10.1371/journal.pone.0070540; citation_id=CR34"/> <meta name="citation_reference" content="citation_journal_title=Trans Am Fish Soc; citation_title=Prevalence of external skin lesions and polycyclic aromatic hydrocarbon concentrations in Gulf of Mexico fishes, post-Deepwater Horizon; citation_author=SA Murawski, WT Hogarth, EB Peebles, L Barbeiri; citation_volume=143; citation_publication_date=2014; citation_pages=1084-1097; citation_id=CR35"/> <meta name="citation_reference" content="Nash UA and Randall M (2015) GIS determination of the area of high-profile (>2 m elevation) reef-top biotope within the Pinnacles polygon- based on USGS high resolution multibeam sonar acoustic backscatter data acquired in 2000. USGS Coastal Ecology and Conservation Research Group, Gainesville, FL. Internal Briefing to NRDA TWG, 21 April 2015. 23 pp"/> <meta name="citation_reference" content="NOAA (2010) Oil spills in coral reefs: planning and response considerations. In: Yender RA, Michel J (eds) US Department of Commerce, National Oceanic and Atmospheric Administration. Silver Spring, MD, pp 2–82"/> <meta name="citation_reference" content="NOAA (2014) Cumulative TCNNA SAR Oiling - Days of Oiling, Environmental Response Management Application. Web application. ERMA Deepwater Horizon MC252. National Oceanic and Atmospheric Administration. Accessed 30 Jan 2015. http://response.restoration.noaa.gov/erma/ "/> <meta name="citation_reference" content="citation_journal_title=Environ Res Lett; citation_title=Marine snow formation in the aftermath of the Deepwater Horizon oil spill in the Gulf of Mexico; citation_author=U Passow, K Ziervogel, V Asper, A Diercks; citation_volume=7; citation_publication_date=2012; citation_pages=035301; citation_doi=10.1088/1748-9326/7/3/035301; citation_id=CR39"/> <meta name="citation_reference" content="citation_journal_title=Cont Shelf Res; citation_title=Correspondence of sea fan orientations with measured currents on hard bottom habitats of the Mississippi/Alabama continental shelf; citation_author=MB Peccini, IR MacDonald; citation_volume=28; citation_publication_date=2008; citation_pages=302-308; citation_doi=10.1016/j.csr.2007.08.003; citation_id=CR40"/> <meta name="citation_reference" content="citation_journal_title=Mar Pollut Bull; citation_title=Bioaccumulation and histopathological effects of oil on a stony coral; citation_author=EC Peters, P Meyers, P Yevich, N Blake; citation_volume=12; citation_publication_date=1981; citation_pages=333-339; citation_doi=10.1016/0025-326X(81)90106-5; citation_id=CR41"/> <meta name="citation_reference" content="Prytherch HF (1983) A descriptive survey of the bottom longline fishery in the Gulf of Mexico. NOAA Technical Memorandum NMFS-SEFC-122:1–33"/> <meta name="citation_reference" content="citation_title=Reefs and Banks of the Northwestern Gulf of Mexico: their geological, biological, and physical dynamics; citation_publication_date=1985; citation_id=CR43; citation_author=R Rezak; citation_author=TJ Bright; citation_author=W McGrail; citation_publisher=John Wiley and Sons"/> <meta name="citation_reference" content="citation_journal_title=Mar Ecol Prog Ser; citation_title=Heterogeneous feeding in benthic suspension feeders: the natural diet and grazing rate of the temperate gorgonian Paramuricea clavata (Cnidaria; Octocorallia) over a year cycle; citation_author=M Ribes, R Coma, JM Gili; citation_volume=183; citation_publication_date=1999; citation_pages=125-137; citation_doi=10.3354/meps183125; citation_id=CR44"/> <meta name="citation_reference" content="citation_journal_title=J Mar Biol Assoc UK; citation_title=Cold-water corals landed by bottom longline fisheries in the Azores (north-eastern Atlantic); citation_author=I Sampaio, A Braga-Henriques, C Pham, O Ocana, V Matos, T Morato, FM Porteiro; citation_volume=92; citation_publication_date=2012; citation_pages=1547-1555; citation_doi=10.1017/S0025315412000045; citation_id=CR45"/> <meta name="citation_reference" content="citation_journal_title=Int J Trop Biol; citation_title=Octocoral densities and mortalities in Gorgona Island, Colombia, Tropical Eastern Pacific; citation_author=JA Sánchez, NE Ardila, J Andrade, LF Dueñas, R Navas, D Ballesteros; citation_volume=62; citation_publication_date=2014; citation_pages=209-219; citation_id=CR46"/> <meta name="citation_reference" content="citation_journal_title=Mar Fish Rev; citation_title=Descriptions of the U.S. Gulf of Mexico reef fish bottom longline and vertical line fisheries based on observer data; citation_author=E Scott-Denton, PF Cryer, JP Gocke, MR Harrelson, DL Kinsella, JR Pulver, RC Smith, JA Williams; citation_volume=72; citation_publication_date=2011; citation_pages=1-26; citation_id=CR47"/> <meta name="citation_reference" content="citation_journal_title=Mar Ecol Prog Ser; citation_title=Stable isotopic composition of deep-sea gorgonian corals Primnoa spp.: a new archive of surface processes; citation_author=OA Sherwood, JM Heikoop, DB Scott, MJ Risk, TP Guilderson, RA McKinney; citation_volume=301; citation_publication_date=2005; citation_pages=135-148; citation_doi=10.3354/meps301135; citation_id=CR48"/> <meta name="citation_reference" content="citation_journal_title=Deep Sea Res Part II Top Stud Oceanogr; citation_title=Coral injuries observed at mesophotic reefs after the Deepwater Horizon oil discharge; citation_author=M Silva, PJ Etnoyer, IR MacDonald; citation_publication_date=2015; citation_id=CR49"/> <meta name="citation_reference" content="Sulak K, Berg J, Randall M, Dennis III GD, Brooks RA (2008) Dual-carbon sources fuel the OCS deep-reef community, a stable isotope investigation. Proc 11th Int Coral Reef Symp 2:945–949"/> <meta name="citation_reference" content="citation_journal_title=J Exp Mar Biol Ecol; citation_title=photoQuad: a dedicated seabed image processing software, and a comparative error analysis of four photoquadrat methods; citation_author=V Trygonis, M Sini; citation_volume=424; citation_publication_date=2012; citation_pages=99-108; citation_doi=10.1016/j.jembe.2012.04.018; citation_id=CR51"/> <meta name="citation_reference" content="citation_journal_title=Proc Natl Acad Sci USA; citation_title=Fallout plume of submerged oil from Deepwater Horizon; citation_author=DL Valentine, GB Fisher, RK Nelson, CM Reddy, SP Sylva, MA Woo; citation_volume=111; citation_publication_date=2014; citation_pages=15906-15911; citation_doi=10.1073/pnas.1414873111; citation_id=CR52"/> <meta name="citation_reference" content="Weaver DC, Dennis GD, Sulak KJ (2002) Community structure and trophic ecology of demersal fishes on the Pinnacles Reef tract. USGS BSR 2001–2008"/> <meta name="citation_reference" content="citation_journal_title=Proc Natl Acad Sci USA; citation_title=Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico; citation_author=HK White, PY Hsing, W Cho, TM Shank, EE Cordes, AM Quattrini, RK Nelson, R Camilli, AWJ Demopoulos, CR German, JM Brooks, HH Roberts, W Shedd, CM Reddy, CR Fisher; citation_volume=109; citation_publication_date=2012; citation_pages=20303-22030; citation_doi=10.1073/pnas.1118029109; citation_id=CR54"/> <meta name="citation_author" content="Etnoyer, Peter J."/> <meta name="citation_author_email" content="peter.etnoyer@noaa.gov"/> <meta name="citation_author_institution" content="NOAA Center for Coastal Environmental Health and Biomolecular Research, Charleston, USA"/> <meta name="citation_author" content="Wickes, Leslie N."/> <meta name="citation_author_institution" content="JHT, Inc., Orlando, USA"/> <meta name="citation_author" content="Silva, Mauricio"/> <meta name="citation_author_institution" content="Florida State University, Tallahassee, USA"/> <meta name="citation_author" content="Dubick, J. D."/> <meta name="citation_author_institution" content="JHT, Inc., Orlando, USA"/> <meta name="citation_author" content="Balthis, Len"/> <meta name="citation_author_institution" content="NOAA Center for Coastal Environmental Health and Biomolecular Research, Charleston, USA"/> <meta name="citation_author" content="Salgado, Enrique"/> <meta name="citation_author_institution" content="JHT, Inc., Orlando, USA"/> <meta name="citation_author" content="MacDonald, Ian R."/> <meta name="citation_author_institution" content="Florida State University, Tallahassee, USA"/> <meta name="format-detection" content="telephone=no"/> <meta name="citation_cover_date" content="2016/03/01"/> <meta property="og:url" content="https://link.springer.com/article/10.1007/s00338-015-1363-2"/> <meta property="og:type" content="article"/> <meta property="og:site_name" content="SpringerLink"/> <meta property="og:title" content="Decline in condition of gorgonian octocorals on mesophotic reefs in the northern Gulf of Mexico: before and after the Deepwater Horizon oil spill - Coral Reefs"/> <meta property="og:description" content="Hard-bottom ‘mesophotic’ reefs along the ‘40-fathom’ (73 m) shelf edge in the northern Gulf of Mexico were investigated for potential effects of the Deepwater Horizon (DWH) oil spill from the Macondo well in April 2010. Alabama Alps Reef, Roughtongue Reef, and Yellowtail Reef were near the well, situated 60–88 m below floating oil discharged during the DWH spill for several weeks and subject to dispersant applications. In contrast, Coral Trees Reef and Madison Swanson South Reef were far from the DWH spill site and below the slick for less than a week or not at all, respectively. The reefs were surveyed by ROV in 2010, 2011, and 2014 and compared to similar surveys conducted one and two decades earlier. Large gorgonian octocorals were present at all sites in moderate abundance including Swiftia exserta, Hypnogorgia pendula, Thesea spp., and Placogorgia spp. The gorgonians were assessed for health and condition in a before-after-control-impact (BACI) research design using still images captured from ROV video transects. Injury was modeled as a categorical response to proximity and time using logistic regression. Condition of gorgonians at sites near Macondo well declined significantly post-spill. Before the spill, injury was observed for 4–9 % of large gorgonians. After the spill, injury was observed in 38–50 % of large gorgonians. Odds of injury for sites near Macondo were 10.8 times higher post-spill, but unchanged at far sites. The majority of marked injured colonies in 2011 declined further in condition by 2014. Marked healthy colonies generally remained healthy. Background stresses to corals, including fishing activity, fishing debris, and coral predation, were noted during surveys, but do not appear to account for the decline in condition at study sites near Macondo well."/> <meta property="og:image" content="https://static-content.springer.com/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig1_HTML.gif"/> <meta name="format-detection" content="telephone=no"> <link rel="apple-touch-icon" sizes="180x180" href=/oscar-static/img/favicons/darwin/apple-touch-icon-92e819bf8a.png> <link rel="icon" type="image/png" sizes="192x192" href=/oscar-static/img/favicons/darwin/android-chrome-192x192-6f081ca7e5.png> <link rel="icon" type="image/png" sizes="32x32" href=/oscar-static/img/favicons/darwin/favicon-32x32-1435da3e82.png> <link rel="icon" type="image/png" sizes="16x16" href=/oscar-static/img/favicons/darwin/favicon-16x16-ed57f42bd2.png> <link rel="shortcut icon" data-test="shortcut-icon" 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Alabama Alps Reef, Roughtongue Reef, and Yellowtail Reef were near the well, situated 60–88 m below floating oil discharged during the DWH spill for several weeks and subject to dispersant applications. In contrast, Coral Trees Reef and Madison Swanson South Reef were far from the DWH spill site and below the slick for less than a week or not at all, respectively. The reefs were surveyed by ROV in 2010, 2011, and 2014 and compared to similar surveys conducted one and two decades earlier. Large gorgonian octocorals were present at all sites in moderate abundance including Swiftia exserta, Hypnogorgia pendula, Thesea spp., and Placogorgia spp. The gorgonians were assessed for health and condition in a before-after-control-impact (BACI) research design using still images captured from ROV video transects. Injury was modeled as a categorical response to proximity and time using logistic regression. Condition of gorgonians at sites near Macondo well declined significantly post-spill. Before the spill, injury was observed for 4–9 % of large gorgonians. After the spill, injury was observed in 38–50 % of large gorgonians. Odds of injury for sites near Macondo were 10.8 times higher post-spill, but unchanged at far sites. The majority of marked injured colonies in 2011 declined further in condition by 2014. Marked healthy colonies generally remained healthy. 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gorgonian octocorals on mesophotic reefs in the northern Gulf of Mexico: before and after the Deepwater Horizon oil spill </div> <div data-test="inCoD" data-track-context="sticky banner"> <div class="c-pdf-container"> <div class="c-pdf-download u-clear-both u-mb-16"> <a href="/content/pdf/10.1007/s00338-015-1363-2.pdf" class="u-button u-button--full-width u-button--primary u-justify-content-space-between c-pdf-download__link" data-article-pdf="true" data-readcube-pdf-url="true" data-test="pdf-link" data-draft-ignore="true" data-track="content_download" data-track-type="article pdf download" data-track-action="download pdf" data-track-label="button" data-track-external download> <span class="c-pdf-download__text">Download PDF</span> <svg aria-hidden="true" focusable="false" width="16" height="16" class="u-icon"><use xlink:href="#icon-eds-i-download-medium"/></svg> </a> </div> </div> </div> </div> </div> <div class="c-article-header"> <header> <ul class="c-article-author-list 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Alabama Alps Reef, Roughtongue Reef, and Yellowtail Reef were near the well, situated 60–88 m below floating oil discharged during the DWH spill for several weeks and subject to dispersant applications. In contrast, Coral Trees Reef and Madison Swanson South Reef were far from the DWH spill site and below the slick for less than a week or not at all, respectively. The reefs were surveyed by ROV in 2010, 2011, and 2014 and compared to similar surveys conducted one and two decades earlier. Large gorgonian octocorals were present at all sites in moderate abundance including <i>Swiftia exserta</i>, <i>Hypnogorgia pendula</i>, <i>Thesea</i> spp., and <i>Placogorgia</i> spp. The gorgonians were assessed for health and condition in a before-after-control-impact (BACI) research design using still images captured from ROV video transects. Injury was modeled as a categorical response to proximity and time using logistic regression. Condition of gorgonians at sites near Macondo well declined significantly post-spill. Before the spill, injury was observed for 4–9 % of large gorgonians. After the spill, injury was observed in 38–50 % of large gorgonians. Odds of injury for sites near Macondo were 10.8 times higher post-spill, but unchanged at far sites. The majority of marked injured colonies in 2011 declined further in condition by 2014. Marked healthy colonies generally remained healthy. Background stresses to corals, including fishing activity, fishing debris, and coral predation, were noted during surveys, but do not appear to account for the decline in condition at study sites near Macondo well.</p></div></div></section> <div data-test="cobranding-download"> </div> <div class="app-card-service" data-test="article-checklist-banner"> <div> <a class="app-card-service__link" data-track="click_presubmission_checklist" data-track-context="article page top of reading companion" data-track-category="pre-submission-checklist" data-track-action="clicked article page checklist banner test 2 old version" data-track-label="link" href="https://beta.springernature.com/pre-submission?journalId=338" data-test="article-checklist-banner-link"> <span class="app-card-service__link-text">Use our pre-submission checklist</span> <svg class="app-card-service__link-icon" aria-hidden="true" focusable="false"><use xlink:href="#icon-eds-i-arrow-right-small"></use></svg> </a> <p class="app-card-service__description">Avoid common mistakes on your manuscript.</p> </div> <div class="app-card-service__icon-container"> <svg class="app-card-service__icon" aria-hidden="true" focusable="false"> <use xlink:href="#icon-eds-i-clipboard-check-medium"></use> </svg> </div> </div> <div class="main-content"> <section data-title="Introduction"><div class="c-article-section" id="Sec1-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Sec1">Introduction</h2><div class="c-article-section__content" id="Sec1-content"><p>The Deepwater Horizon oil spill released 4.3 million barrels of crude oil near 1500 m depth in the Gulf of Mexico over a period of 87 d in the spring of 2010 from the Macondo well MC 252 (McNutt et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2011" title="McNutt MK, Camilli R, Crone TJ, Guthrie GD, Hsieh PA, Ryerson TB, Savas O, Shaffer F (2011) Review of flow rate estimates of the Deepwater Horizon oil spill. Proc Natl Acad Sci USA 109:20260–20267" href="/article/10.1007/s00338-015-1363-2#ref-CR33" id="ref-link-section-d48549525e457">2011</a>). The oil formed a large subsurface plume and produced a large surface slick that was visible by airplane and satellite for nearly 90 d (NOAA <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="NOAA (2014) Cumulative TCNNA SAR Oiling - Days of Oiling, Environmental Response Management Application. Web application. ERMA Deepwater Horizon MC252. National Oceanic and Atmospheric Administration. Accessed 30 Jan 2015. 
 http://response.restoration.noaa.gov/erma/
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR38" id="ref-link-section-d48549525e460">2014</a>). The biological footprint of the DWH spill on infaunal sediments has been calculated as 148 km<sup>2</sup> (Montagna et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2013" title="Montagna PA, Baguley JG, Cooksey C, Hartwell I, Hyde LJ, Hyland JL, Kalke RD, Kracker LM, Reuscher M, Rhodes ACE (2013) Deep-sea benthic footprint of the Deepwater Horizon blowout. PLoS One 8:e70540" href="/article/10.1007/s00338-015-1363-2#ref-CR34" id="ref-link-section-d48549525e465">2013</a>), but recent studies indicate that the chemical footprint of impacted areas may be substantially larger than previously anticipated (Valentine et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Valentine DL, Fisher GB, Nelson RK, Reddy CM, Sylva SP, Woo MA (2014) Fallout plume of submerged oil from Deepwater Horizon. Proc Natl Acad Sci USA 111:15906–15911" href="/article/10.1007/s00338-015-1363-2#ref-CR52" id="ref-link-section-d48549525e468">2014</a>). There is a potential that the biological footprint of the spill, including injury to corals, could increase with increasing search effort (Fisher et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Fisher CR, Demopoulos AWJ, Cordes EE, Baums IB, White HK, Bourque JR (2014) Coral communities as indicators of ecosystem-level impacts of the deepwater horizon spill. Bioscience 64:796–807" href="/article/10.1007/s00338-015-1363-2#ref-CR19" id="ref-link-section-d48549525e472">2014</a>).</p><p>The surface oil slick from the DWH blowout persisted in the northern Gulf of Mexico for several weeks over a series of deep-water rocky reefs called the Pinnacle Trend (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig1">1</a>). Fishermen refer to these as the ‘broken grounds’ (Gittings et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1992" title="Gittings SR, Bright TJ, Schroeder WW, Sager WW, Laswell SJ, Rezak R (1992) Invertebrate assemblages and ecological controls on topographic features in the Northeast Gulf of Mexico. Bull Mar Sci 50:435–455" href="/article/10.1007/s00338-015-1363-2#ref-CR21" id="ref-link-section-d48549525e481">1992</a>). The rocky plateaus occur along the 60- to 90-m depth contours between the Mississippi Delta and Pensacola, FL. Recreational and commercial fishermen recognize these ‘40-fathom’ reefs as good fishing habitat for snapper and grouper, conferring social and economic value on these natural resources (Prytherch <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1983" title="Prytherch HF (1983) A descriptive survey of the bottom longline fishery in the Gulf of Mexico. NOAA Technical Memorandum NMFS-SEFC-122:1–33" href="/article/10.1007/s00338-015-1363-2#ref-CR42" id="ref-link-section-d48549525e484">1983</a>; Scott-Denton et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2011" title="Scott-Denton E, Cryer PF, Gocke JP, Harrelson MR, Kinsella DL, Pulver JR, Smith RC, Williams JA (2011) Descriptions of the U.S. Gulf of Mexico reef fish bottom longline and vertical line fisheries based on observer data. Mar Fish Rev 72:1–26" href="/article/10.1007/s00338-015-1363-2#ref-CR47" id="ref-link-section-d48549525e487">2011</a>).</p><div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-1" data-title="Fig. 1"><figure><figcaption><b id="Fig1" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 1</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/1" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig1_HTML.gif?as=webp"><img aria-describedby="Fig1" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig1_HTML.gif" alt="figure 1" loading="lazy"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-1-desc"><p>A map showing the surface oil slick generated by the Deepwater Horizon blowout at Macondo wellhead (<i>gray triangle</i>), overlaid with the location of four mesophotic reefs assessed in this study (<i>yellow circles</i>)—Alabama Alps Reef (AAR), Roughtongue Reef (RTR), Coral Trees Reef (CTR), and Madison Swanson South Reef (MSSR). Yellowtail Reef is adjacent to RTR</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/1" data-track-dest="link:Figure1 Full size image" aria-label="Full size image figure 1" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> <p>There are nine named reefs in the Pinnacle Trend (Gardner et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2000" title="Gardner JV, Sulak KJ, Dartnell P, Hellequin L, Calder B, Mayer LA (2000) Cruise report RV Ocean Surveyor Cruise O-1-00-GM the bathymetry and acoustic backscatter of the Pinnacles Area, Northern Gulf of Mexico May 23, through June 10, 2000. Venice, LA to Venice, LA. U.S. Geological Survey Open-File Report 00-350" href="/article/10.1007/s00338-015-1363-2#ref-CR20" id="ref-link-section-d48549525e518">2000</a>). Among the largest of these are Alabama Alps Reef (AAR), Roughtongue Reef (RTR), and Yellowtail Reef (YTR), which were situated below the spill for 19–39 d (NOAA <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="NOAA (2014) Cumulative TCNNA SAR Oiling - Days of Oiling, Environmental Response Management Application. Web application. ERMA Deepwater Horizon MC252. National Oceanic and Atmospheric Administration. Accessed 30 Jan 2015. 
 http://response.restoration.noaa.gov/erma/
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR38" id="ref-link-section-d48549525e521">2014</a>). AAR (39 d), RTR (19 d), and YTR (19 d) are 57–109 km from the well. The reefs have been surveyed by remotely operated vehicle (ROV) since 1989. Two other reefs about 200 km to the east of the Macondo wellhead, Madison Swanson South Reef and Coral Trees Reefs, were below the slick for 0–3 d, and surveyed by ROV since 1997.</p><p>Gorgonian octocorals (sea fans), black corals, and scleractinian corals occur on all of these reefs, providing structure and refuge for small demersal fishes that are prey to larger fishes (Weaver et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2002" title="Weaver DC, Dennis GD, Sulak KJ (2002) Community structure and trophic ecology of demersal fishes on the Pinnacles Reef tract. USGS BSR 2001–2008" href="/article/10.1007/s00338-015-1363-2#ref-CR53" id="ref-link-section-d48549525e527">2002</a>). The corals contribute substantially to biodiversity in high relief areas on the reefs (Gittings et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1992" title="Gittings SR, Bright TJ, Schroeder WW, Sager WW, Laswell SJ, Rezak R (1992) Invertebrate assemblages and ecological controls on topographic features in the Northeast Gulf of Mexico. Bull Mar Sci 50:435–455" href="/article/10.1007/s00338-015-1363-2#ref-CR21" id="ref-link-section-d48549525e530">1992</a>). Suspension feeding corals are vulnerable to oil and dispersants (NOAA <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2010" title="NOAA (2010) Oil spills in coral reefs: planning and response considerations. In: Yender RA, Michel J (eds) US Department of Commerce, National Oceanic and Atmospheric Administration. Silver Spring, MD, pp 2–82" href="/article/10.1007/s00338-015-1363-2#ref-CR37" id="ref-link-section-d48549525e533">2010</a>) so these animals were considered as part of the Natural Resource Damage Assessment (NRDA) for DWH. Injury to gorgonian octocorals has been demonstrated adjacent to the wellhead, and this injury has been linked to DWH (White et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e536">2012</a>) for sites up to 22 km from the Macondo well (Fisher et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Fisher CR, Demopoulos AWJ, Cordes EE, Baums IB, White HK, Bourque JR (2014) Coral communities as indicators of ecosystem-level impacts of the deepwater horizon spill. Bioscience 64:796–807" href="/article/10.1007/s00338-015-1363-2#ref-CR19" id="ref-link-section-d48549525e539">2014</a>). Additional findings have linked numerous injured coral colonies observed in the Pinnacle Trend to exposure to DWH oil (Silva et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="Silva M, Etnoyer PJ, MacDonald IR (2015) Coral injuries observed at mesophotic reefs after the Deepwater Horizon oil discharge. Deep Sea Res Part II Top Stud Oceanogr. doi:
 10.1016/j.dsr2.2015.05.013
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR49" id="ref-link-section-d48549525e543">2015</a>).</p><p>Deep-water gorgonian octocorals (Alcyonacea) and black corals (Antipatharia) are common and conspicuous in the 60–90 m ‘mesophotic’ depth range in the northern Gulf of Mexico, from Texas to Florida (Bayer <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1961" title="Bayer FM (1961) The shallow-water Octocorallia of the West Indian region: a manual for marine biologists. Martinus Nijhoff, The Hague" href="/article/10.1007/s00338-015-1363-2#ref-CR2" id="ref-link-section-d48549525e550">1961</a>; Rezak et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1985" title="Rezak R, Bright TJ, McGrail W (1985) Reefs and Banks of the Northwestern Gulf of Mexico: their geological, biological, and physical dynamics. John Wiley and Sons, New York" href="/article/10.1007/s00338-015-1363-2#ref-CR43" id="ref-link-section-d48549525e553">1985</a>; Cairns and Bayer <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2009" title="Cairns SD, Bayer FM (2009) Octocorallia (Cnidaria) of the Gulf of Mexico. In: Felder DL, Camp DK (eds) Gulf of Mexico–origins, waters, and biota, vol 1., BiodiversityTexas A&M Press, College Station, pp 321–331" href="/article/10.1007/s00338-015-1363-2#ref-CR6" id="ref-link-section-d48549525e556">2009</a>). The gorgonians are important components of deep-reef habitat because they provide structural complexity, substrate, and refuge for fish and invertebrates (Krieger and Wing <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2002" title="Krieger KJ, Wing BL (2002) Megafauna associations with deepwater corals (Primnoa spp.) in the Gulf of Alaska. Hydrobiologia 471:83–90" href="/article/10.1007/s00338-015-1363-2#ref-CR29" id="ref-link-section-d48549525e559">2002</a>; Buhl-Mortensen and Mortensen <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2005" title="Buhl-Mortensen L, Mortensen PB (2005) Distribution and diversity of species associated with deep-sea gorgonian corals off Atlantic Canada. In: Freiwald A, Roberts JM (eds) Cold-water corals and ecosystems. Springer, Heidelberg, pp 849–879" href="/article/10.1007/s00338-015-1363-2#ref-CR5" id="ref-link-section-d48549525e562">2005</a>). The soft coral colonies grow on rocky, current-swept habitat, and prefer high relief features.</p><p>Gorgonian octocorals on the Pinnacle Trend reefs are predominantly heterotrophic suspension feeders. Their branches grow in a flabellate, reticulated (sea fan) shape with an orientation perpendicular to prevailing currents in order to maximize particle flux and rates of capture for the polyps along the branches (Peccini and MacDonald <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2008" title="Peccini MB, MacDonald IR (2008) Correspondence of sea fan orientations with measured currents on hard bottom habitats of the Mississippi/Alabama continental shelf. Cont Shelf Res 28:302–308" href="/article/10.1007/s00338-015-1363-2#ref-CR40" id="ref-link-section-d48549525e568">2008</a>). These are not technically ‘mesophotic coral ecosystems’ (sensu Kahng et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2010" title="Kahng SE, Garcia-Sais JR, Spalding HL, Brokovich E, Wagner D, Weil E, Hinderstein L, Toonen RJ (2010) Community ecology of mesophotic coral reef systems. Coral Reefs 29:255–275" href="/article/10.1007/s00338-015-1363-2#ref-CR27" id="ref-link-section-d48549525e571">2010</a>) because the corals are azooxanthellate, but we refer to them as ‘mesophotic reefs’ because they occur in the deeper portion of the photic zone.</p><p>Gorgonian octocorals and antipatharians are susceptible to exposure to water-borne toxicants because they are large, emergent epifauna with polyps adapted to straining small particles of food from the water column. The diet and prey of these heterotrophic corals consist of holoplankton—small particles of phytoplankton and zooplankton. Stable isotope studies indicate that the colonies feed on surface-originated and re-suspended photosynthetic material (Sherwood et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2005" title="Sherwood OA, Heikoop JM, Scott DB, Risk MJ, Guilderson TP, McKinney RA (2005) Stable isotopic composition of deep-sea gorgonian corals Primnoa spp.: a new archive of surface processes. Mar Ecol Prog Ser 301:135–148" href="/article/10.1007/s00338-015-1363-2#ref-CR48" id="ref-link-section-d48549525e577">2005</a>; Sulak et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2008" title="Sulak K, Berg J, Randall M, Dennis III GD, Brooks RA (2008) Dual-carbon sources fuel the OCS deep-reef community, a stable isotope investigation. Proc 11th Int Coral Reef Symp 2:945–949" href="/article/10.1007/s00338-015-1363-2#ref-CR50" id="ref-link-section-d48549525e580">2008</a>). Transport of surface particulates to deep-water corals can be rapid (Davies et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2009" title="Davies AJ, Duineveld GC, Lavaleye MS, Bergman MJ, van Haren H, Roberts JM (2009) Downwelling and deep-water bottom currents as food supply mechanisms to the cold-water coral Lophelia pertusa (Scleractinia) at the Mingulay Reef complex. Limnol Oceanogr 54:620–629" href="/article/10.1007/s00338-015-1363-2#ref-CR13" id="ref-link-section-d48549525e583">2009</a>).</p><p>The most likely mechanism for oil/dispersant exposure to the reefs would be passive or active sinking of contaminated surface material toward the benthos (Passow et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="Passow U, Ziervogel K, Asper V, Diercks A (2012) Marine snow formation in the aftermath of the Deepwater Horizon oil spill in the Gulf of Mexico. Environ Res Lett 7:035301" href="/article/10.1007/s00338-015-1363-2#ref-CR39" id="ref-link-section-d48549525e589">2012</a>) and/or re-suspension and advection of contaminated surface material in sediments from the reef perimeter onto the reef. These processes may have been intensified by storm events during the time when floating oil was present over the reef sites (Silva et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="Silva M, Etnoyer PJ, MacDonald IR (2015) Coral injuries observed at mesophotic reefs after the Deepwater Horizon oil discharge. Deep Sea Res Part II Top Stud Oceanogr. doi:
 10.1016/j.dsr2.2015.05.013
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR49" id="ref-link-section-d48549525e592">2015</a>). Multiple studies have shown deleterious effects of oil and dispersant exposure on coral colonies and coral larvae (e.g., Loya and Rinkevich <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1980" title="Loya YM, Rinkevich B (1980) Effects of oil pollution on coral reef communities. Mar Ecol Prog Ser 3:167–180" href="/article/10.1007/s00338-015-1363-2#ref-CR31" id="ref-link-section-d48549525e595">1980</a>; Goodbody-Gringley et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2013" title="Goodbody-Gringley G, Wetzel DL, Gillon D, Pulster E, Miller A, Ritchie KB (2013) Toxicity of deepwater horizon source oil and the chemical dispersant, Corexit® 9500, to coral larvae. PLoS One 8:e45574" href="/article/10.1007/s00338-015-1363-2#ref-CR22" id="ref-link-section-d48549525e598">2013</a>; DeLeo et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="DeLeo DM, Ruiz-Ramos DV, Baums IB, Cordes EE (2015) Response of deep-water corals to oil and chemical dispersant exposure. Deep Sea Res Part II Top Stud Oceanogr. doi:
 10.1016/j.dsr2.2015.02.028
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR14" id="ref-link-section-d48549525e601">2015</a>). Scleractinian coral tissue exposed to oil will break down (Johannes <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1975" title="Johannes RE (1975) Pollution and degradation of coral reef communities. In: Johannes RE, Ferguson Wood EJ (eds) Tropical marine pollution. Elsevier, Amsterdam, pp 13–51" href="/article/10.1007/s00338-015-1363-2#ref-CR26" id="ref-link-section-d48549525e605">1975</a>), resulting in a thinning of coenosarc cell layers (Peters et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1981" title="Peters EC, Meyers P, Yevich P, Blake N (1981) Bioaccumulation and histopathological effects of oil on a stony coral. Mar Pollut Bull 12:333–339" href="/article/10.1007/s00338-015-1363-2#ref-CR41" id="ref-link-section-d48549525e608">1981</a>). The few studies that examined octocorals noted deleterious effects including polyp mortality and discoloration (DeLeo et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="DeLeo DM, Ruiz-Ramos DV, Baums IB, Cordes EE (2015) Response of deep-water corals to oil and chemical dispersant exposure. Deep Sea Res Part II Top Stud Oceanogr. doi:
 10.1016/j.dsr2.2015.02.028
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR14" id="ref-link-section-d48549525e611">2015</a>; Silva et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="Silva M, Etnoyer PJ, MacDonald IR (2015) Coral injuries observed at mesophotic reefs after the Deepwater Horizon oil discharge. Deep Sea Res Part II Top Stud Oceanogr. doi:
 10.1016/j.dsr2.2015.05.013
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR49" id="ref-link-section-d48549525e614">2015</a>), inhibition of metamorphosis (Kushmora et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1997" title="Kushmora A, Henning G, Hofmann DK, Benayahu Y (1997) Metamorphosis of Heteroxenia fuscescens planulae (Cnidaria: Octocorallia) is inhibited by crude oil: a novel short term toxicity bioassay. Mar Environ Res 43:295–302" href="/article/10.1007/s00338-015-1363-2#ref-CR30" id="ref-link-section-d48549525e617">1997</a>), and behavioral changes (Cohen et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1977" title="Cohen Y, Nissenbaum A, Eisler R (1977) Effects of Iranian crude oil on the Red Sea octocoral Heteroxenia fuscescens. Environ Pollut 12:173–186" href="/article/10.1007/s00338-015-1363-2#ref-CR10" id="ref-link-section-d48549525e620">1977</a>).</p><p>Octocorals may be exposed to contaminants through the ingestion of contaminated plankton or suspended organic material. Octocorals ingest a wide variety of food resources, including zooplankton (Coma et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1994" title="Coma R, Gili JM, Zabala M, Riera T (1994) Feeding and prey capture cycles in the aposymbiontic gorgonian Paramuricea clavata. Mar Ecol Prog Ser 115:257–270" href="/article/10.1007/s00338-015-1363-2#ref-CR11" id="ref-link-section-d48549525e626">1994</a>), detrital particulate organic matter (POM), nanoeukaryotes, ciliates (Ribes et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1999" title="Ribes M, Coma R, Gili JM (1999) Heterogeneous feeding in benthic suspension feeders: the natural diet and grazing rate of the temperate gorgonian Paramuricea clavata (Cnidaria; Octocorallia) over a year cycle. Mar Ecol Prog Ser 183:125–137" href="/article/10.1007/s00338-015-1363-2#ref-CR44" id="ref-link-section-d48549525e629">1999</a>), and phytoplankton (Fabricius et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1995" title="Fabricius KE, Genin A, Benayahu Y (1995) Flow-dependent herbivory and growth in zooxanthellae-free soft corals. Limnol Oceanogr 40:1290–1301" href="/article/10.1007/s00338-015-1363-2#ref-CR17" id="ref-link-section-d48549525e632">1995</a>). Zooplankton can bio-accumulate toxic polycyclic aromatic hydrocarbons (Almeda et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Almeda R, Baca S, Hyatt C, Buskey EJ (2014) Ingestion and sublethal effects of physically and chemically dispersed crude oil on marine planktonic copepods. Ecotoxicology 23:988–1003" href="/article/10.1007/s00338-015-1363-2#ref-CR1" id="ref-link-section-d48549525e635">2014</a>) and exhibit isotopic field signatures that indicate oil-derived hydrocarbons have entered the food web (Graham et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2010" title="Graham WM, Condon RH, Carmichael RH, D’Ambra I, Patterson HK, Linn LJ, Hernandez FJ Jr (2010) Oil carbon entered the coastal planktonic food web during the Deepwater Horizon oil spill. Environ Res Lett 5:e045301" href="/article/10.1007/s00338-015-1363-2#ref-CR23" id="ref-link-section-d48549525e638">2010</a>). Effects of ingesting contaminated material by coral have not yet been studied.</p><p>Gorgonian octocoral colonies are good subjects of study to test for effects from the DWH spill because there is a precedent for injury to gorgonians from this oil spill (White et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e644">2012</a>; Silva et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="Silva M, Etnoyer PJ, MacDonald IR (2015) Coral injuries observed at mesophotic reefs after the Deepwater Horizon oil discharge. Deep Sea Res Part II Top Stud Oceanogr. doi:
 10.1016/j.dsr2.2015.05.013
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR49" id="ref-link-section-d48549525e647">2015</a>). Furthermore, there is a potential for exposure to suspended and falling particles, and the reliance of these colonies on surface POM suggests a strong connection to surface plankton. This study asks: is there evidence of injury to gorgonian octocorals on mesophotic reefs near DWH? What types of injury were observed and to what degree? How does condition of gorgonians compare between sites near and far? Was there a change in condition to gorgonians before and after the DWH oil spill? We provide this information to assess potential injury to biota in the mesophotic zone relative to the DWH oil spill in the Gulf of Mexico.</p></div></div></section><section data-title="Methods"><div class="c-article-section" id="Sec2-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Sec2">Methods</h2><div class="c-article-section__content" id="Sec2-content"><h3 class="c-article__sub-heading" id="Sec3">Field methods</h3><p>A before-after-control-impact (BACI) research design was used to study demersal fish and coral populations 50–100 m deep between Louisiana and West Florida at sites near and far from the Macondo well MC252 as part of the NRDA for DWH. BACI is a powerful research design that allows for detection of change over time. Two ‘impact sites,’ Alabama Alps Reef (AAR) and Roughtongue Reef/Yellowtail Reef (RTR/YTR), were near the Macondo well within 109 km of the wellhead, 60–88 m below floating oil discharged during the DWH spill for >19 d, and were subject to dispersant applications (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig1">1</a>). In contrast, two other ‘control’ or ‘reference sites’ called Coral Trees Reef (CTR) and Madison Swanson South Reef (MSSR) were far from the Macondo well (~200 km), below the surface slick for <3 d or not at all, respectively, and were not subject to dispersant application. RTR and YTR were treated as one impact site because they are directly adjacent (~1.5 km apart) with similar crest depths (Table <a data-track="click" data-track-label="link" data-track-action="table anchor" href="/article/10.1007/s00338-015-1363-2#Tab1">1</a>).</p><div class="c-article-table" data-test="inline-table" data-container-section="table" id="table-1"><figure><figcaption class="c-article-table__figcaption"><b id="Tab1" data-test="table-caption">Table 1 Study site characteristics</b></figcaption><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="table-link" data-track="click" data-track-action="view table" data-track-label="button" rel="nofollow" href="/article/10.1007/s00338-015-1363-2/tables/1" aria-label="Full size table 1"><span>Full size table</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> <p>The reefs were surveyed before the spill in 1989 (MAMES: Brooks <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1991" title="Brooks JM (ed) (1991) Mississippi-Alabama continental shelf ecosystem study: data summary and synthesis. OCS Study MMS 91-0062. U.S. Department of the Interior, Minerals Management Service, Gulf of Mexico OCS Regional Office, New Orleans" href="/article/10.1007/s00338-015-1363-2#ref-CR4" id="ref-link-section-d48549525e1112">1991</a>) and 1997–2003 (USGS: Weaver et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2002" title="Weaver DC, Dennis GD, Sulak KJ (2002) Community structure and trophic ecology of demersal fishes on the Pinnacles Reef tract. USGS BSR 2001–2008" href="/article/10.1007/s00338-015-1363-2#ref-CR53" id="ref-link-section-d48549525e1115">2002</a>) and then after the spill, in 2010, 2011 and 2014 as part of the NRDA effort (Table <a data-track="click" data-track-label="link" data-track-action="table anchor" href="/article/10.1007/s00338-015-1363-2#Tab2">2</a>). Remotely operated vehicles (ROVs) equipped with still and video cameras were used to conduct slow moving, low altitude, 2–5 min video transects on the reeftop biotope. The survey methods supported a BACI research design to compare condition and abundance of corals at mesophotic sites near and far from DWH, before and after the oil spill.</p><div class="c-article-table" data-test="inline-table" data-container-section="table" id="table-2"><figure><figcaption class="c-article-table__figcaption"><b id="Tab2" data-test="table-caption">Table 2 Cruise expeditions that contributed video for health assessment of corals</b></figcaption><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="table-link" data-track="click" data-track-action="view table" data-track-label="button" rel="nofollow" href="/article/10.1007/s00338-015-1363-2/tables/2" aria-label="Full size table 2"><span>Full size table</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> <p>Pre-spill video transect methods were comparable to post-spill methods, but standard definition cameras used pre-spill were generally lower resolution than high-definition cameras used after the spill. Full details on the ROVs and cameras are provided as Electronic Supplementary Materials (ESM—ROV Methods). Together the USGS, MAMES, and NRDA datasets comprised 295 min of pre-spill video to compare with 340 min of post-spill video in 2011. By combining these various datasets, a minimum of 30 min of high-quality transect video was available for each reef before and after DWH oil spill (ESM Fig. S1). While transect time differed among sites and time periods, total observations were comparable with large enough samples to facilitate robust statistical analyses based on proportions (see ‘<a data-track="click" data-track-label="link" data-track-action="section anchor" href="/article/10.1007/s00338-015-1363-2#Sec5">Statistical methods</a>’ section). Variable transect times were included as a continuous covariate in the statistical model with near and far sites run separately, thereby adjusting for any potential effect of effort.</p><p>All octocorals appearing along transects in 2011 were enumerated for analyses of species abundance and diversity. A group of select taxa were identified for assessment of health and condition based on discernible species and condition. Species that attain large colony sizes (>20 cm in mean height or width) were readily identifiable in standard and high-definition video, and their condition could be assessed (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig2">2</a>). The species used in health analyses included <i>Swiftia exserta</i>, <i>Hypnogorgia pendula</i>, <i>Thesea nivea</i>, <i>T. rubra</i>, <i>Placogorgia</i> spp., <i>Paramuricea</i> spp., and <i>Muriceides</i> cf. <i>M. hirta</i>. <i>Paramuricea</i> and <i>Muriceides</i> could not be distinguished from <i>Placogorgia</i> spp. in video and still images based on gross morphology, and were thus binned together for health analyses. <i>Placogorgia</i> were most common among the yellow plexaurid specimens collected, particularly <i>P. rudis</i>. A species identification guide to large gorgonian taxa is provided in ESM—Species guide. Small gorgonians (<i>Nicella</i> and <i>Bebryce</i> spp.) were difficult to discern in video due to image quality, colony size, and reproducibility of results, so these taxa were not assessed.</p><div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-2" data-title="Fig. 2"><figure><figcaption><b id="Fig2" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 2</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/2" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig2_HTML.jpg?as=webp"><img aria-describedby="Fig2" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig2_HTML.jpg" alt="figure 2" loading="lazy"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-2-desc"><p>Healthy and injured colonies of the gorgonian octocorals <i>Swiftia exserta</i> (<b>a</b>, <b>b</b>), <i>Hypnogorgia pendula</i> (<b>c</b>, <b>d</b>), and <i>Placogorgia</i> sp. (<b>e</b>, <b>f</b>) similar in appearance to <i>Paramuricea</i> </p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/2" data-track-dest="link:Figure2 Full size image" aria-label="Full size image figure 2" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> <p>Due to differences in species composition among sites, the large, discernible gorgonian species were binned together into a single group (large gorgonians) for the BACI analysis. These were treated as one generic taxon in order to achieve comparable sample sizes among reef sites. This treatment rests on the assumption that the largest gorgonians at different sites have similar exposure to currents and similar feeding modes.</p><p>To monitor change in injury to gorgonians over time, weighted nylon rope markers flagged with syntactic foam and reflective tape were deployed at 12 locations in 2011. Deployments included six markers at AAR and six at RTR. Of these 12 markers, 11 were re-photographed in 2014. Gorgonian corals at each flagged location were photographed in 2011 with an AquaSLR digital still camera and then again in 2014 using a DPC-8800. The time-series images sought to reproduce ROV heading, camera angle, zoom, and lighting.</p><h3 class="c-article__sub-heading" id="Sec4">Laboratory methods</h3><p>A total of 153 biological samples of octocorals were collected by ROV in 2010 (<i>n</i> = 31), 2011 (<i>n</i> = 58), and 2014 (<i>n</i> = 64) for the purposes of species identification and/or chemical analyses. Specimens were documented and preserved according to established protocols (Etnoyer et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2006" title="Etnoyer P, Cairns SD, Sanchez JA, Reed JK, Lopez JV, Schroeder WW, Brooke SD, Watling L, Baco-Taylor A, Williams GC, Lindner A, France SC, Bruckner AW (2006) Deep-sea coral collection protocols. NOAA Technical Memorandum NMFS-OPR-28, Silver Spring" href="/article/10.1007/s00338-015-1363-2#ref-CR16" id="ref-link-section-d48549525e2142">2006</a>). Morphological diagnosis was achieved by scanning electron microscopy (JEOL JSM5600LV) and light microscopy (Olympus SZX16). Genus-level determination was based upon the criteria set forth by Bayer (<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1981" title="Bayer FM (1981) Key to the genera of Octocorallia exclusive of Pennatulacea (Coelenterata: Anthozoa), with diagnoses of new taxa. Proc Biol Soc Wash 94:902–947" href="/article/10.1007/s00338-015-1363-2#ref-CR3" id="ref-link-section-d48549525e2145">1981</a>). Species-level identifications were made using the best available keys, and species identities were confirmed through consultation with experts at Smithsonian National Museum of Natural History (See ESM Figs. S4–S22).</p><p>For image-based analyses of in situ coral health and condition, target taxa were identified from video and a framegrab was generated for detailed assessment of health and condition. Health assessment was a three-stage process. Framegrabs were selected from each sequence so as to optimize resolution and visibility of the coral colonies being assessed. In the second review, gorgonians were ‘verified,’ i.e., assessed for discernible condition. Gorgonians were ‘discernible’ when the whole colony was clearly visible (e.g., cloudy water, fish, or colony orientation did not obscure large parts). In the third stage, injured and healthy regions on gorgonians were outlined using the software photoQuad (Trygonis and Sini <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="Trygonis V, Sini M (2012) photoQuad: a dedicated seabed image processing software, and a comparative error analysis of four photoquadrat methods. J Exp Mar Biol Ecol 424:99–108" href="/article/10.1007/s00338-015-1363-2#ref-CR51" id="ref-link-section-d48549525e2151">2012</a>). Injury impact rankings (degree of injury) were assigned based on the categorical scale (0–4) described by White et al. (<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e2154">2012</a>) where ‘0’ denoted healthy and ‘4’ indicated >90 % damage.</p><p>Injured colonies were categorized both in terms of their degree and type of injury. Types of injury included bare or denuded branches, broken branches, overgrowth, abnormal polyps, and/or severe discoloration. In the case of broken branches, the flabellum shape and condition were referenced to observed patterns of branching for the given taxa, and then breakage was coded at the holdfast, primary or secondary branch. Degree of injury was upweighted (increased by one level) when there was clear evidence of major branch loss.</p><h3 class="c-article__sub-heading" id="Sec5">Statistical methods</h3><h4 class="c-article__sub-heading c-article__sub-heading--small" id="Sec6">Community composition</h4><p>The nonparametric analysis of similarity (ANOSIM) global R statistic (Clarke and Green <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1988" title="Clarke KR, Green RH (1988) Statistical design and analysis for a ‘biological effects’ study. Mar Ecol Prog Ser 46:213–226" href="/article/10.1007/s00338-015-1363-2#ref-CR8" id="ref-link-section-d48549525e2172">1988</a>) was employed to test the null hypothesis of no difference in species composition among reef sites using non-metric multidimensional scaling techniques (nMDS) with an underlying Bray–Curtis similarity matrix based on counts of morphospecies along transects in 2011. Similarity percentages (SIMPER) were calculated using Bray–Curtis distance to identify the species groups contributing significantly to the differences among groups near and far from DWH. Multivariate analyses were conducted using the software PRIMER 6.1 (Clarke and Gorley <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2006" title="Clarke KR, Gorley RN (2006) PRIMER v6: user manual/tutorial. PRIMER-E, Plymouth" href="/article/10.1007/s00338-015-1363-2#ref-CR9" id="ref-link-section-d48549525e2175">2006</a>). Univariate analyses employed the nonparametric Kruskal–Wallis statistic with Tukey’s honest significant difference (HSD) post hoc test to test the null hypothesis of no difference in frequency of occurrence for small and large gorgonians, fishing gear, and gear interactions.</p><h4 class="c-article__sub-heading c-article__sub-heading--small" id="Sec7">Health assessment</h4><p>The health assessment considered an ordered, categorical range of injuries by degree of intensity (0–4; White et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e2186">2012</a>) in a BACI design. The categorical scale for injury was 0 = healthy colony, <1 % injury; 1 = 1–10 % injury; 2 = 10–50 %; 3 = 50–90 %; and 4 = >90 % (White et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e2189">2012</a>). These ranks were used for consistency with other DWH-NRDA studies (White et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e2192">2012</a>; Hsing et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2013" title="Hsing P-Y, Fu B, Larcom EA, Berlet SP, Shank TM, Govindarajan AF, Lukasiewicz AJ, Dixon PM, Fisher CR (2013) Evidence of lasting impact of the Deepwater Horizon oil spill on a deep Gulf of Mexico coral community. Elem Sci Anthr 1:0000012" href="/article/10.1007/s00338-015-1363-2#ref-CR25" id="ref-link-section-d48549525e2195">2013</a>; Fisher et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Fisher CR, Demopoulos AWJ, Cordes EE, Baums IB, White HK, Bourque JR (2014) Coral communities as indicators of ecosystem-level impacts of the deepwater horizon spill. Bioscience 64:796–807" href="/article/10.1007/s00338-015-1363-2#ref-CR19" id="ref-link-section-d48549525e2198">2014</a>). The values were pooled together in two ways. Injury was considered in the binary sense, as absent (0) or present (1–4), and as low ranks (0, 1) or high ranks (2, 3, 4). Dead gorgonian stolons and toppled colonies were treated in a separate class or injury rank (=4) as appropriate. The marker study also used an ordered categorical response, in the form of 0 = healthy, 1 = injured, 2 = more injured.</p><p>Severity of injury to large gorgonians was modeled as a function of proximity to Macondo well (near and far) and time (post-spill vs. pre-spill). Injury was treated as a binary response (logit, dependent variable) using logistic regression to obtain odds ratio estimates and Wald confidence intervals. The predictor variables were proximity and time frame, and the response variable was presence or absence of injury. Transect duration was included as a continuous covariate to adjust for variable transect lengths.</p><p>A sign test was used to measure the direction of change (from 2011 to 2014) in injured status for marked colonies revisited in 2014. The test statistic was calculated from the difference in injury (injury 2014–injury 2011) to evaluate the direction of change in status (more injured, less injured, or no change).</p></div></div></section><section data-title="Results"><div class="c-article-section" id="Sec8-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Sec8">Results</h2><div class="c-article-section__content" id="Sec8-content"><h3 class="c-article__sub-heading" id="Sec9">Community composition</h3><p>At least 31 octocoral species were collected (Table <a data-track="click" data-track-label="link" data-track-action="table anchor" href="/article/10.1007/s00338-015-1363-2#Tab3">3</a>). Biological species richness ranged from nine species at CTR to 17 species at RTR. Morphospecies richness in video samples ranged from six at RTR to 11 at AAR. Species composition in video was significantly different among sites (ANOSIM test, Global R = 0.66, <i>p</i> < 0.01) and among groups near and far from DWH (ANOSIM test, Global R = 0.40, <i>p</i> < 0.01). Small gorgonian octocorals <i>Bebryce</i> spp., <i>Nicella</i> sp., and <i>Villogorgia</i> sp. contributed a cumulative 51.6 % to the dissimilarity among distance groups, while large gorgonians <i>S. exserta</i> and <i>Placogorgia</i> spp. contributed an additional 18 % to the dissimilarity among distance groups.</p><div class="c-article-table" data-test="inline-table" data-container-section="table" id="table-3"><figure><figcaption class="c-article-table__figcaption"><b id="Tab3" data-test="table-caption">Table 3 Octocoral species identified in sample collections 2010–2014</b></figcaption><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="table-link" data-track="click" data-track-action="view table" data-track-label="button" rel="nofollow" href="/article/10.1007/s00338-015-1363-2/tables/3" aria-label="Full size table 3"><span>Full size table</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> <p>No single species occurred in equivalent abundance at all study sites. Small colonies <20 cm (e.g., <i>Bebryce</i> and <i>Nicella</i> spp.) were highly abundant at some sites and relatively rare at others. The frequency of small fans was significantly different among sites (Kruskal–Wallis, <i>p</i> < 0.001). The frequencies of large gorgonians were more consistent among sites (<i>p</i> = 0.05; ESM Fig. S2). Maximum densities of large gorgonians ranged from 12 to 48 colonies 100 m<sup>−2</sup> (Table <a data-track="click" data-track-label="link" data-track-action="table anchor" href="/article/10.1007/s00338-015-1363-2#Tab1">1</a>).</p><h3 class="c-article__sub-heading" id="Sec10">Health assessment</h3><h4 class="c-article__sub-heading c-article__sub-heading--small" id="Sec11">Was there a change in condition to gorgonian octocorals before and after the DWH oil spill?</h4><p>There was a significant change in condition to gorgonian octocorals before and after the oil spill at sites below the surface oil slick (<i>p</i> < 0.0001, OR = 7.0–16.7; Table <a data-track="click" data-track-label="link" data-track-action="table anchor" href="/article/10.1007/s00338-015-1363-2#Tab4">4</a>). Odds of injury after the spill were 10.8 times higher than injury before the spill for all injury types (all ranks 1–4; Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig3">3</a>a) and 6.9 times higher for injuries >10 % (ranks ≥2; Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig3">3</a>b). The historical injury rate for large gorgonians at impact sites was 4.3 % (9/210) at RTR/YTR and 8.9 % (24/271) at AAR, with all taxa pooled together (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig4">4</a>). Injury rates after the spill increased to 38 % (35/93) and 50 % (105/210) for RTR/YTR and AAR, respectively. Injury was documented across all taxa, ranging from 21 % (5/24) of <i>Placogorgia</i> spp. to 62 % (35/56) of <i>Thesea</i> spp. post-spill (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig5">5</a>).</p><div class="c-article-table" data-test="inline-table" data-container-section="table" id="table-4"><figure><figcaption class="c-article-table__figcaption"><b id="Tab4" data-test="table-caption">Table 4 Summary statistics showing results of logistic regression models</b></figcaption><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="table-link" data-track="click" data-track-action="view table" data-track-label="button" rel="nofollow" href="/article/10.1007/s00338-015-1363-2/tables/4" aria-label="Full size table 4"><span>Full size table</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> <div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-3" data-title="Fig. 3"><figure><figcaption><b id="Fig3" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 3</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/3" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig3_HTML.gif?as=webp"><img aria-describedby="Fig3" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig3_HTML.gif" alt="figure 3" loading="lazy"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-3-desc"><p>Odds ratio estimates (<i>circles</i>) with 95 % confidence intervals (<i>error bars</i>) for odds of injury post-spill versus odds of injury pre-spill at sites near and far from Macondo wellhead. <b>a</b> Odds ratios with rank 0 = healthy, and ranks 1–4 = injury. <b>b</b> Odds ratios with ranks 0, 1 = healthy; and ranks 2–4 = injury. The <i>dashed line</i> at <i>y</i> = 1 indicates no significant difference from pre-spill</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/3" data-track-dest="link:Figure3 Full size image" aria-label="Full size image figure 3" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> <div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-4" data-title="Fig. 4"><figure><figcaption><b id="Fig4" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 4</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/4" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig4_HTML.gif?as=webp"><img aria-describedby="Fig4" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig4_HTML.gif" alt="figure 4" loading="lazy"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-4-desc"><p>Overall frequency of injury to large gorgonians, with all ranks (1–4) combined, before and after Deepwater Horizon oil spill at the Macondo well</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/4" data-track-dest="link:Figure4 Full size image" aria-label="Full size image figure 4" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> <div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-5" data-title="Fig. 5"><figure><figcaption><b id="Fig5" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 5</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/5" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig5_HTML.gif?as=webp"><img aria-describedby="Fig5" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig5_HTML.gif" alt="figure 5" loading="lazy"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-5-desc"><p>Frequency of injury post-spill for four large sea fan taxa (<i>Thesea</i> spp., <i>Swiftia exserta</i>, <i>Hypnogorgia pendula</i>, and <i>Placogorgia</i> spp.) occurring on northwest Gulf of Mexico mesophotic reefs. <i>Asterisks</i> indicate taxa were absent on the transects</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/5" data-track-dest="link:Figure5 Full size image" aria-label="Full size image figure 5" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> <p>Before the spill, the condition of gorgonians at sites AAR and RTR/YTR near DWH was healthy compared to far sites (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig4">4</a>). The odds of injured gorgonians far from the wellhead were historically 7.7 (<i>p</i> < 0.0001, OR = 4.6–13.0) times higher than the odds of injured gorgonians near DWH. Pooling taxa at reference sites yield historical injury rates of 28.4 % (92/324) and 10.8 % (24/222), for Madison Swanson South Reef (MSSR) and Coral Trees (CTR), respectively. Pooling reference sites (CTR/MSSR) showed a moderate increase in gorgonian injury from 21 % pre-spill to 26 % post-spill. The change in condition at reference sites was not significantly different (<i>p</i> = 0.651, OR = 0.7–1.7) before and after the spill (Table <a data-track="click" data-track-label="link" data-track-action="table anchor" href="/article/10.1007/s00338-015-1363-2#Tab4">4</a>, Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig3">3</a>a, b).</p><p>Taken together, these results indicate a significant effect of time only at sites near the wellhead; gorgonian condition did not change significantly at sites far from the wellhead (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig6">6</a>). Before the spill, reference sites were degraded relative to impact sites, but after the spill the pattern reversed, with the odds of injured gorgonians near DWH 2.5 (=1/0.4) times higher than far sites.</p><div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-6" data-title="Fig. 6"><figure><figcaption><b id="Fig6" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 6</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/6" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig6_HTML.gif?as=webp"><img aria-describedby="Fig6" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig6_HTML.gif" alt="figure 6" loading="lazy"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-6-desc"><p>Frequency of observation for each degree of injury (0 = <1 %, 1 = 1–10 %, 2 = 10–50 %, 3 = 50–90 %, 4 = >90 %) for large gorgonians on study sites near and far from Macondo well, before and after the Deepwater Horizon spill</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/6" data-track-dest="link:Figure6 Full size image" aria-label="Full size image figure 6" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> <p>Several different statistical models were tested: an ordinal logistic regression model (OLRM) using all five ordered response (impact ranking) categories; a binary logistic regression model (LRM0) defining healthy versus injured as impact ranking 0 versus (1, 2, 3, 4) combined, and a binary logistic regression model (LRM1) defining healthy versus injured as impact rankings (0, 1) versus (2, 3, 4) combined. Each of the various models gave the same interpretation and similar odds ratio (OR). The binary logistic regression model was presented, due to concerns about low cell frequencies for some of the impact ranking categories. Goodness-of-fit tests suggested some degree of lack of model fit, although receiver operating characteristic (ROC) curves indicate reasonably good predictive power for sites near the wellhead (area under the ROC curve ranged from 0.74 to 0.78).</p><h4 class="c-article__sub-heading c-article__sub-heading--small" id="Sec12">What types of injury were observed and to what degree?</h4><p>Types of injury to large gorgonians were similar among sites near and far, both pre-spill and post-spill. While most injured gorgonian colonies exhibited more than one form of injury, the most common <i>primary</i> forms of injury across all sites post-spill were overgrowth (47 %), broken branches (12 %), or bare branches (10 %). Frequency of primary injury types across all sites was similar pre-spill, with examples of overgrowth (49 %), broken branches (26 %), and bare branches (14 %). Overgrowth consisted primarily of hydroids or sedimented material. Bare branches had clear loss of the coenosarc and polyps, with only the axis remaining. Post-spill, overgrowth was also the predominant form of injury at sites both near (48 %) and far (45 %). Less common forms of injury included severe discoloration, eroded polyps, and toppled colonies. Severe discoloration was unusually dark tissue of brown or gray (<i>S. exserta</i>, <i>H. pendula</i> and <i>Placogorgia</i> spp.) or unusually light tissue with erosion of polyps (<i>T. nivea</i>).</p><h4 class="c-article__sub-heading c-article__sub-heading--small" id="Sec13">Was there a change in condition to marked colonies after the DWH oil spill?</h4><p>Colonies at the marked locations included six <i>Hypnogorgia</i> and 12 <i>Swiftia.</i> Of the 18 total marked colonies, eight were considered healthy and ten injured in 2011. Marked corals imaged in both 2011 and 2014 (<i>n</i> = 18) indicated that most healthy corals remained healthy (7/8 = 88 %) and most colonies that were injured in 2011 progressed in injury (8/10 = 80 %). The null hypothesis of no change was rejected (<i>p</i> < 0.01, two-sided sign test), and the test statistic was positive, indicating a direction of change from less injured to more injured. Injuries included loss of tissue, expanded overgrowth, pruning of branches, and toppling of the colony. One injured colony of <i>Swiftia</i> remained at the same level of injury. In the only case where a colony progressed from injured to healthy, injury in 2011 was to 4 % of the <i>Swiftia</i> colony in overgrowth on secondary branches. The location of initial injury appeared to have some influence on the rate of injury progression. For example, injury of <3 % at the base of a <i>Hypnogorgia</i> colony resulted in rapid progression of overgrowth, loss of branches, and collapse of the colony. Thus, low percentages of injury (3–4 %) could be followed subsequently by either colony recovery or colony collapse (Fig. <a data-track="click" data-track-label="link" data-track-action="figure anchor" href="/article/10.1007/s00338-015-1363-2#Fig7">7</a>).</p><div class="c-article-section__figure js-c-reading-companion-figures-item" data-test="figure" data-container-section="figure" id="figure-7" data-title="Fig. 7"><figure><figcaption><b id="Fig7" class="c-article-section__figure-caption" data-test="figure-caption-text">Fig. 7</b></figcaption><div class="c-article-section__figure-content"><div class="c-article-section__figure-item"><a class="c-article-section__figure-link" data-test="img-link" data-track="click" data-track-label="image" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/7" rel="nofollow"><picture><source type="image/webp" srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig7_HTML.jpg?as=webp"><img aria-describedby="Fig7" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1007%2Fs00338-015-1363-2/MediaObjects/338_2015_1363_Fig7_HTML.jpg" alt="figure 7" loading="lazy"></picture></a></div><div class="c-article-section__figure-description" data-test="bottom-caption" id="figure-7-desc"><p>Two colonies of <i>Hypnogorgia pendula</i> marked in 2011 (<b>a</b>, <b>c</b>) and revisited in 2014 (<b>b</b>, <b>d</b>). <i>Green outlines</i> indicate healthy sections, and <i>red outlines</i> indicate injured sections</p></div></div><div class="u-text-right u-hide-print"><a class="c-article__pill-button" data-test="article-link" data-track="click" data-track-label="button" data-track-action="view figure" href="/article/10.1007/s00338-015-1363-2/figures/7" data-track-dest="link:Figure7 Full size image" aria-label="Full size image figure 7" rel="nofollow"><span>Full size image</span><svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-chevron-right-small"></use></svg></a></div></figure></div> </div></div></section><section data-title="Discussion"><div class="c-article-section" id="Sec14-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Sec14">Discussion</h2><div class="c-article-section__content" id="Sec14-content"><p>This study found clear evidence of injury to at least four species of gorgonian octocorals on mesophotic reefs situated below the surface oil slick from the Deepwater Horizon blowout at Macondo wellhead. Results indicate a highly significant decline in condition at sites nearest the wellhead after the spill. Decline in condition was severe, from <10 % of colonies injured before 2003 to more than 50 % of colonies injured in 2011. Nearly all injured colonies marked in 2011 declined in condition, suggesting that recovery of corals is unlikely. The majority of healthy colonies marked in 2011 remained healthy, suggesting that the cause of injury was ephemeral. Injury occurred in all taxa of large gorgonians, including <i>S. exserta</i>, <i>H. pendula</i>, <i>Thesea</i> spp., and <i>Placogorgia</i> spp., in the form of eroded polyps, discoloration, bare branches, overgrowth, missing branches, and broken branches.</p><p>The findings are consistent with the results of other studies showing benthic impacts to deep-sea corals from the Deepwater Horizon oil spill (White et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e3078">2012</a>; Hsing et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2013" title="Hsing P-Y, Fu B, Larcom EA, Berlet SP, Shank TM, Govindarajan AF, Lukasiewicz AJ, Dixon PM, Fisher CR (2013) Evidence of lasting impact of the Deepwater Horizon oil spill on a deep Gulf of Mexico coral community. Elem Sci Anthr 1:0000012" href="/article/10.1007/s00338-015-1363-2#ref-CR25" id="ref-link-section-d48549525e3081">2013</a>; Fisher et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Fisher CR, Demopoulos AWJ, Cordes EE, Baums IB, White HK, Bourque JR (2014) Coral communities as indicators of ecosystem-level impacts of the deepwater horizon spill. Bioscience 64:796–807" href="/article/10.1007/s00338-015-1363-2#ref-CR19" id="ref-link-section-d48549525e3084">2014</a>; Silva et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="Silva M, Etnoyer PJ, MacDonald IR (2015) Coral injuries observed at mesophotic reefs after the Deepwater Horizon oil discharge. Deep Sea Res Part II Top Stud Oceanogr. doi:
 10.1016/j.dsr2.2015.05.013
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR49" id="ref-link-section-d48549525e3087">2015</a>). Injuries to <i>Paramuricea</i> gorgonians were linked to oil from the Macondo well by White et al. (<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e3094">2012</a>). Those results were later extended to other sites up to 22 km from the wellhead (Fisher et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Fisher CR, Demopoulos AWJ, Cordes EE, Baums IB, White HK, Bourque JR (2014) Coral communities as indicators of ecosystem-level impacts of the deepwater horizon spill. Bioscience 64:796–807" href="/article/10.1007/s00338-015-1363-2#ref-CR19" id="ref-link-section-d48549525e3097">2014</a>). These studies reported a patchy distribution of injury (i.e., not mass mortality) with healthy and injured colonies co-occurring, much like the observations presented here. A decline in condition of marked injured gorgonians (<i>n</i> = 19) was also reported near 1500 m depth (Hsing et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2013" title="Hsing P-Y, Fu B, Larcom EA, Berlet SP, Shank TM, Govindarajan AF, Lukasiewicz AJ, Dixon PM, Fisher CR (2013) Evidence of lasting impact of the Deepwater Horizon oil spill on a deep Gulf of Mexico coral community. Elem Sci Anthr 1:0000012" href="/article/10.1007/s00338-015-1363-2#ref-CR25" id="ref-link-section-d48549525e3103">2013</a>). This temporal decline is consistent with marker observations (<i>n</i> = 18) in the mesophotic zone. These studies near Macondo wellhead were able to produce evidence linking the DWH spill by ‘fingerprinting’ oiled sediments recovered from the branches and polyps of gorgonians (White et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e3109">2012</a>).</p><p>One key difference between this and other studies of deep-sea corals (White et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e3115">2012</a>; Fisher et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Fisher CR, Demopoulos AWJ, Cordes EE, Baums IB, White HK, Bourque JR (2014) Coral communities as indicators of ecosystem-level impacts of the deepwater horizon spill. Bioscience 64:796–807" href="/article/10.1007/s00338-015-1363-2#ref-CR19" id="ref-link-section-d48549525e3118">2014</a>) is that this is the first to employ a BACI design. The context gained through this approach was demonstrable evidence of pre-spill injury to gorgonians at reference sites far from the wellhead, nearly 200 km away. It is unlikely injuries at reference sites can be attributed to DWH because injuries to large gorgonians were apparent in the earliest ROV surveys of the reefs available to the study in 1997. Additionally, condition of colonies was unchanged pre- and post-spill, in contrast to the dramatic changes observed at reefs near Macondo well. Reference sites were under surface slicks for <3 d or not at all.</p><p>The most probable cause of injury to corals on reference sites was bottom-contact fishing. Bottom-contact fishing is a known stressor to deep-sea coral habitats and the likely source of pre-spill injury at reference sites. Bottom longlines in particular are recognized as a threat to gorgonian octocorals (Sampaio et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="Sampaio I, Braga-Henriques A, Pham C, Ocana O, De Matos V, Morato T, Porteiro FM (2012) Cold-water corals landed by bottom longline fisheries in the Azores (north-eastern Atlantic). J Mar Biol Assoc UK 92:1547–1555" href="/article/10.1007/s00338-015-1363-2#ref-CR45" id="ref-link-section-d48549525e3124">2012</a>). Monofilament line occurred in and around gorgonian colonies, with significantly more line and more interactions observed at reference sites (Kruskal–Wallis, <i>p</i> < 0.01; ESM Fig. S3).</p><p>Coral Trees Reef and Madison Swanson South Reef are both situated in a region known as the ‘northern grounds’ for the Gulf of Mexico bottom longline fishery. The northern grounds are “the area of the Gulf where fishing actually began in the 1800’s” (Prytherch <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 1983" title="Prytherch HF (1983) A descriptive survey of the bottom longline fishery in the Gulf of Mexico. NOAA Technical Memorandum NMFS-SEFC-122:1–33" href="/article/10.1007/s00338-015-1363-2#ref-CR42" id="ref-link-section-d48549525e3134">1983</a>). Intense fishing pressure at MSSR led to closure in 2000, when the Fishery Management Council put all bottom-contact gear off limits. Low levels of bottom longline fishing effort were reported from the Pinnacles region in 2001, but the vast majority of effort was concentrated in the eastern Gulf, near Tampa, Florida (Scott-Denton et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2011" title="Scott-Denton E, Cryer PF, Gocke JP, Harrelson MR, Kinsella DL, Pulver JR, Smith RC, Williams JA (2011) Descriptions of the U.S. Gulf of Mexico reef fish bottom longline and vertical line fisheries based on observer data. Mar Fish Rev 72:1–26" href="/article/10.1007/s00338-015-1363-2#ref-CR47" id="ref-link-section-d48549525e3137">2011</a>). Observations of fishing line on transects at Pinnacle Trend sites were few. Impact sites are not likely to have incurred higher levels of injury from fishing than reference sites in a relatively brief time frame.</p><p>Octocorals are subject to a wide variety of natural and anthropogenic stressors beyond fisheries. Known stressors to octocorals include disease, ocean warming, predation by corallivores, toppling by hurricanes, and sedimentation. These alternatives are recognized here as counterpoint explanations for potential causes of injury unrelated to the Deepwater Horizon incident.</p><p>While fungal infections have been shown to eradicate gorgonian populations in tropical waters (Kim and Harvell <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2004" title="Kim K, Harvell D (2004) The rise and fall of a six year coral fungal epizootic. Am Nat 164(S5):S52–S63" href="/article/10.1007/s00338-015-1363-2#ref-CR28" id="ref-link-section-d48549525e3146">2004</a>), few diseases have been reported for deep-water octocorals. Disease has not been reported from Gulf mesophotic taxa to our knowledge. Furthermore, disease outbreaks are typically reported to occur in one or a few host taxa (e.g., <i>Gorgonia ventalina</i>, <i>Eunicella verrucosa;</i> Hall-Spencer et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2007" title="Hall-Spencer JM, Pike J, Munn CB (2007) Diseases affect cold-water corals too: Eunicella verrucosa (Cnidaria: Gorgonacea) necrosis in SW England. Dis Aquat Org 76:87–97" href="/article/10.1007/s00338-015-1363-2#ref-CR24" id="ref-link-section-d48549525e3155">2007</a>) and are not ubiquitous to a reef. The indiscriminate nature of the observed injuries suggests that disease is not the cause of injury at impact sites.</p><p>Octocorals are susceptible to surface warming in shallow waters (Sánchez et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Sánchez JA, Ardila NE, Andrade J, Dueñas LF, Navas R, Ballesteros D (2014) Octocoral densities and mortalities in Gorgona Island, Colombia, Tropical Eastern Pacific. Int J Trop Biol 62:209–219" href="/article/10.1007/s00338-015-1363-2#ref-CR46" id="ref-link-section-d48549525e3161">2014</a>), but there is presently no evidence of warming-related injury to octocorals in deep water. A dramatic warming event occurred in 2005 that caused bleaching to 40–60 % of shallow water corals at Flower Garden Banks in the western Gulf of Mexico, but no observations were deeper than 30 m and no mortality was evident (Eakin et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2010" title="Eakin CM, Morgan JA, Heron SF, Smith TB, Liu G, Alvarez- Filip L, Baca B, Bartels E, Bastidas C, Bouchon C, Brandt M, Bruckner AW, Bunkley-Williams L, Cameron A, Causey B, Chiappone M, Christensen TRL, Crabbe MJC, Day O, de la Guardia E, Díaz-Pulido G, DiResta D, Gil-Agudelo D, Gilliam DS, Ginsburg RN, Gore S, Guzmán HM, Hendee JC, Hernández-Delgado EA, Husain E, Jeffrey CFG, Jones RJ, Jordán-Dahlgren E, Kaufman LS, Kline DI, Kramer PA, Lang JC, Lirman D, Mallela J, Manfrino C, Maréchal JP, Marks K, Mihaly J, Miller WJ, Mueller EM, Orozco Toro CA, Oxenford HA, Ponce-Taylor D, Quinn N, Ritchie KB, Rodríguez S, Rodríguez Ramírez A, Romano S, Samhouri JF, Sánchez JA, Schmahl GP, Shank BV, Skirving WJ, Steiner SCC, Villamizar E, Walsh SM, Walter C, Weil E, Williams EH, Woody Roberson K, Yusuf Y (2010) Caribbean corals in crisis: record thermal stress, bleaching, and mortality in 2005. PLoS One 5(11):e13969" href="/article/10.1007/s00338-015-1363-2#ref-CR15" id="ref-link-section-d48549525e3164">2010</a>). Maps of temperature anomalies indicate impact sites were minimally affected. The reduced severity of the anomalies and depth of the mesophotic reefs suggest that surface warming is not the cause.</p><p>Corallivore predators can occur in deep water (Mah et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2010" title="Mah C, Nizinski M, Lundsten L (2010) Phylogenetic revision of the Hippasterinae (Goniasteridae; Asteroidea): systematics of deep sea corallivores, including one new genus and three new species. Zool J Linn Soc 160:266–301" href="/article/10.1007/s00338-015-1363-2#ref-CR32" id="ref-link-section-d48549525e3170">2010</a>), but they do not explain the injuries observed in the Gulf of Mexico. Predators of gorgonian octocorals include asteroid sea stars, flamingo tongue snails, gastropods, and cidaroid urchins, among others. However, there were no obvious infestations of any of these predators in pre- or post-spill video. The frequency of occurrence for predators was very low among sites, with no obvious examples of predator-inflicted injury.</p><p>Hurricanes and storms can impact shallow coral reefs in the Caribbean, and topple gorgonian octocoral colonies attached to poorly consolidated substrate. Multiple hurricanes have passed over the Pinnacles sites in the past few decades, but the reef depths should provide an insulating effect from surface winds. Hurricanes Ivan and Katrina both passed near to the Pinnacles sites as strong Category 4 storms, in 2004 and 2005, respectively, yet other storms of similar strength (Andrew in 1992 and Opal in 1995) occurred prior to the pre-spill surveys, and the effects of those storms were not apparent in pre-spill surveys. It is not likely that hurricanes were the cause of injury observed in 2011, as prior hurricanes were not associated with subsequent damage to the mesophotic reefs.</p><p>Other studies have invoked DWH as the causative agent for declines in the mesophotic zone, including lesions observed in deep-water crabs (Felder et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Felder DL, Thoma BP, Schmidt WE, Sauvage T, Self-Krayesky SL, Chistoserdov A, Bracken-Grissom HD, Fredericq S (2014) Seaweeds and decapod crustaceans on Gulf deep banks after the Macondo oil spill. BioScience 64:808–819" href="/article/10.1007/s00338-015-1363-2#ref-CR18" id="ref-link-section-d48549525e3180">2014</a>) and fishes (Murawski et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Murawski SA, Hogarth WT, Peebles EB, Barbeiri L (2014) Prevalence of external skin lesions and polycyclic aromatic hydrocarbon concentrations in Gulf of Mexico fishes, post-Deepwater Horizon. Trans Am Fish Soc 143:1084–1097" href="/article/10.1007/s00338-015-1363-2#ref-CR35" id="ref-link-section-d48549525e3183">2014</a>) and up to 50 % injury to a broad range of corals on Pinnacle Trend reefs (Silva et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="Silva M, Etnoyer PJ, MacDonald IR (2015) Coral injuries observed at mesophotic reefs after the Deepwater Horizon oil discharge. Deep Sea Res Part II Top Stud Oceanogr. doi:
 10.1016/j.dsr2.2015.05.013
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR49" id="ref-link-section-d48549525e3186">2015</a>). The latter analysis included black corals and sea whips and used a different dataset of high-resolution still images from another set of pre-spill surveys, reaching the same conclusions presented here.</p><p>Unlike other studies documenting DWH impacts to deep-sea corals (White et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030" href="/article/10.1007/s00338-015-1363-2#ref-CR54" id="ref-link-section-d48549525e3192">2012</a>; Fisher et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Fisher CR, Demopoulos AWJ, Cordes EE, Baums IB, White HK, Bourque JR (2014) Coral communities as indicators of ecosystem-level impacts of the deepwater horizon spill. Bioscience 64:796–807" href="/article/10.1007/s00338-015-1363-2#ref-CR19" id="ref-link-section-d48549525e3195">2014</a>), the current study of mesophotic corals at sites 50–100 km from DWH did not include fingerprinting of ‘Macondo 252’ oil on injured octocorals nor in sediments, but some chemical analyses were conducted. Total polycyclic aromatic hydrocarbons (PAHs) in tissues of corals on Pinnacle Trend reefs were observed in 2010 at low levels (Silva et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="Silva M, Etnoyer PJ, MacDonald IR (2015) Coral injuries observed at mesophotic reefs after the Deepwater Horizon oil discharge. Deep Sea Res Part II Top Stud Oceanogr. doi:
 10.1016/j.dsr2.2015.05.013
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR49" id="ref-link-section-d48549525e3198">2015</a>). Cooksey et al. (<a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Cooksey C, Hyland J, Fulton M, Balthis L, Wirth E, Wade T (2014) Ecological condition of coastal waters along the U.S. Continental Shelf of the Northeastern Gulf of Mexico: 2010. NOAA Technical Memorandum NOS NCCOS 188. NOAA National Ocean Service, Charleston" href="/article/10.1007/s00338-015-1363-2#ref-CR12" id="ref-link-section-d48549525e3201">2014</a>) also reported total PAHs in shelf sediments after the spill (August 2010) at very low levels, below sediment quality bio-effect guidelines developed with data for estuarine species. However, the low levels of PAHs observed near Pinnacle Trend were slightly higher compared to eastern sites with sandy sediments. There are no well-established bio-effect levels for offshore sediments (Cooksey et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Cooksey C, Hyland J, Fulton M, Balthis L, Wirth E, Wade T (2014) Ecological condition of coastal waters along the U.S. Continental Shelf of the Northeastern Gulf of Mexico: 2010. NOAA Technical Memorandum NOS NCCOS 188. NOAA National Ocean Service, Charleston" href="/article/10.1007/s00338-015-1363-2#ref-CR12" id="ref-link-section-d48549525e3204">2014</a>), nor deep-water octocorals, so it may be possible that bio-effect thresholds for estuarine invertebrates are higher than thresholds for deep-water species. Furthermore, dispersants may have also played a role in the development of injury (DeLeo et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="DeLeo DM, Ruiz-Ramos DV, Baums IB, Cordes EE (2015) Response of deep-water corals to oil and chemical dispersant exposure. Deep Sea Res Part II Top Stud Oceanogr. doi:
 10.1016/j.dsr2.2015.02.028
 
 
 " href="/article/10.1007/s00338-015-1363-2#ref-CR14" id="ref-link-section-d48549525e3208">2015</a>).</p><p>The lack of accumulation of DWH-related hydrocarbons in nearby shelf sediments does not preclude the possibility of DWH-related impacts to mesophotic corals located in these same areas. It is likely that oil and dispersant particles would have been patchily distributed in the water column this far from the wellhead (Valentine et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Valentine DL, Fisher GB, Nelson RK, Reddy CM, Sylva SP, Woo MA (2014) Fallout plume of submerged oil from Deepwater Horizon. Proc Natl Acad Sci USA 111:15906–15911" href="/article/10.1007/s00338-015-1363-2#ref-CR52" id="ref-link-section-d48549525e3214">2014</a>). Therefore, oil contaminants would be unlikely to accumulate in large quantities on the sediment surface, but suspended material could still cause harm to suspension feeders as it came into contact with upright corals.</p><p>In the absence of compelling evidence attributable to other known potential sources of injury, the oil slick from Deepwater Horizon remains the likely causative agent for injury observed on mesophotic reefs in the Pinnacle Trend. The persistence and proximity of the surface slick and dispersant applications, as well as evidence of oil-contaminated marine snow (Passow et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2012" title="Passow U, Ziervogel K, Asper V, Diercks A (2012) Marine snow formation in the aftermath of the Deepwater Horizon oil spill in the Gulf of Mexico. Environ Res Lett 7:035301" href="/article/10.1007/s00338-015-1363-2#ref-CR39" id="ref-link-section-d48549525e3220">2012</a>) and seafloor deposition of surface-derived carbon (Chanton et al. <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2014" title="Chanton J, Zhao T, Rosenheim BE, Joye S, Bosman S, Brunner C, Yeager KM, Diercks AR, Hollander D (2014) Using natural abundance radiocarbon to trace the flux of petrocarbon to the seafloor following the Deepwater Horizon oil spill. Environ Sci Technol 49:847–854" href="/article/10.1007/s00338-015-1363-2#ref-CR7" id="ref-link-section-d48549525e3223">2014</a>) all suggest a pathway for contaminants to reach the mesophotic reefs. Contact of surface slick-derived oil or dispersants with mesophotic octocorals may have occurred directly with sinking of oil or oil-contaminated material, or indirectly, through ingestion of contaminated plankton or suspended organic material.</p><p>In conclusion, there is clear evidence of injury and decline to gorgonian octocorals at sites that were situated below the surface slick in the spring of 2010. The degree of injury is substantial, with up to 50 % of sea fans affected; the time frame suggests that the Macondo blowout was a likely causative agent of the decline. Other potential sources of injury, including fisheries, hurricanes, water temperature, and predators, were included in analyses, but do not appear to account for changes to octocoral health at sites in the Pinnacle Trend.</p><p>Alabama Alps Reef, Roughtongue Reef, and Yellowtail Reef were selected as representative sites in this study, but are only a few of many reefs known to harbor octocorals and abundant fishes in the Pinnacle Trend. Recent GIS-derived areas of potential reef habitat indicate that the study sites evaluated may encompass <3 % of total mesophotic reef area in the mapped area below the slick (Nash and Randall <a data-track="click" data-track-action="reference anchor" data-track-label="link" data-test="citation-ref" aria-label="Reference 2015" title="Nash UA and Randall M (2015) GIS determination of the area of high-profile (>2 m elevation) reef-top biotope within the Pinnacles polygon- based on USGS high resolution multibeam sonar acoustic backscatter data acquired in 2000. USGS Coastal Ecology and Conservation Research Group, Gainesville, FL. Internal Briefing to NRDA TWG, 21 April 2015. 23 pp" href="/article/10.1007/s00338-015-1363-2#ref-CR36" id="ref-link-section-d48549525e3232">2015</a>). The results presented here may vastly underestimate the extent of impacts to mesophotic reefs in the northern Gulf of Mexico.</p><p>This study identifies multiple potential sources of injury to gorgonian octocorals of the Gulf of Mexico mesophotic reefs, both within and outside the scope of the Deepwater Horizon oil spill. Monitoring to date suggests recovery to injured colonies is unlikely. Nevertheless, a number of healthy colonies still remain on these reefs, and data suggest that healthy colonies will remain so in the absence of new stressors. Habitat protection and habitat restoration efforts are recommended to ameliorate collateral damage from fishing and to accelerate the recovery rates to gorgonian aggregations in the northern Gulf of Mexico; deep-water monitoring programs should be established to document the progress and effect of the new conservation measures.</p></div></div></section> </div> <div id="MagazineFulltextArticleBodySuffix"><section aria-labelledby="Bib1" data-title="References"><div class="c-article-section" id="Bib1-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Bib1">References</h2><div class="c-article-section__content" id="Bib1-content"><div data-container-section="references"><ul class="c-article-references" data-track-component="outbound reference" data-track-context="references section"><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR1">Almeda R, Baca S, Hyatt C, Buskey EJ (2014) Ingestion and sublethal effects of physically and chemically dispersed crude oil on marine planktonic copepods. Ecotoxicology 23:988–1003</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="noopener" data-track-label="10.1007/s10646-014-1242-6" data-track-item_id="10.1007/s10646-014-1242-6" data-track-value="article reference" data-track-action="article reference" href="https://link.springer.com/doi/10.1007/s10646-014-1242-6" aria-label="Article reference 1" data-doi="10.1007/s10646-014-1242-6">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4078238" aria-label="PubMed Central reference 1">PubMed Central</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2cXms1ajtrc%3D" aria-label="CAS reference 1">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=24756329" aria-label="PubMed reference 1">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 1" href="http://scholar.google.com/scholar_lookup?&title=Ingestion%20and%20sublethal%20effects%20of%20physically%20and%20chemically%20dispersed%20crude%20oil%20on%20marine%20planktonic%20copepods&journal=Ecotoxicology&doi=10.1007%2Fs10646-014-1242-6&volume=23&pages=988-1003&publication_year=2014&author=Almeda%2CR&author=Baca%2CS&author=Hyatt%2CC&author=Buskey%2CEJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR2">Bayer FM (1961) The shallow-water Octocorallia of the West Indian region: a manual for marine biologists. Martinus Nijhoff, The Hague</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 2" href="http://scholar.google.com/scholar_lookup?&title=The%20shallow-water%20Octocorallia%20of%20the%20West%20Indian%20region%3A%20a%20manual%20for%20marine%20biologists&publication_year=1961&author=Bayer%2CFM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR3">Bayer FM (1981) Key to the genera of Octocorallia exclusive of Pennatulacea (Coelenterata: Anthozoa), with diagnoses of new taxa. Proc Biol Soc Wash 94:902–947</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 3" href="http://scholar.google.com/scholar_lookup?&title=Key%20to%20the%20genera%20of%20Octocorallia%20exclusive%20of%20Pennatulacea%20%28Coelenterata%3A%20Anthozoa%29%2C%20with%20diagnoses%20of%20new%20taxa&journal=Proc%20Biol%20Soc%20Wash&volume=94&pages=902-947&publication_year=1981&author=Bayer%2CFM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR4">Brooks JM (ed) (1991) Mississippi-Alabama continental shelf ecosystem study: data summary and synthesis. OCS Study MMS 91-0062. U.S. Department of the Interior, Minerals Management Service, Gulf of Mexico OCS Regional Office, New Orleans</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 4" href="http://scholar.google.com/scholar_lookup?&title=Mississippi-Alabama%20continental%20shelf%20ecosystem%20study%3A%20data%20summary%20and%20synthesis.%20OCS%20Study%20MMS%2091-0062&publication_year=1991"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR5">Buhl-Mortensen L, Mortensen PB (2005) Distribution and diversity of species associated with deep-sea gorgonian corals off Atlantic Canada. In: Freiwald A, Roberts JM (eds) Cold-water corals and ecosystems. Springer, Heidelberg, pp 849–879</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="noopener" data-track-label="10.1007/3-540-27673-4_44" data-track-item_id="10.1007/3-540-27673-4_44" data-track-value="chapter reference" data-track-action="chapter reference" href="https://link.springer.com/doi/10.1007/3-540-27673-4_44" aria-label="Chapter reference 5" data-doi="10.1007/3-540-27673-4_44">Chapter</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 5" href="http://scholar.google.com/scholar_lookup?&title=Distribution%20and%20diversity%20of%20species%20associated%20with%20deep-sea%20gorgonian%20corals%20off%20Atlantic%20Canada&doi=10.1007%2F3-540-27673-4_44&pages=849-879&publication_year=2005&author=Buhl-Mortensen%2CL&author=Mortensen%2CPB"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR6">Cairns SD, Bayer FM (2009) Octocorallia (Cnidaria) of the Gulf of Mexico. In: Felder DL, Camp DK (eds) Gulf of Mexico–origins, waters, and biota, vol 1., BiodiversityTexas A&M Press, College Station, pp 321–331</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 6" href="http://scholar.google.com/scholar_lookup?&title=Octocorallia%20%28Cnidaria%29%20of%20the%20Gulf%20of%20Mexico&pages=321-331&publication_year=2009&author=Cairns%2CSD&author=Bayer%2CFM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR7">Chanton J, Zhao T, Rosenheim BE, Joye S, Bosman S, Brunner C, Yeager KM, Diercks AR, Hollander D (2014) Using natural abundance radiocarbon to trace the flux of petrocarbon to the seafloor following the Deepwater Horizon oil spill. Environ Sci Technol 49:847–854</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1021/es5046524" data-track-item_id="10.1021/es5046524" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1021%2Fes5046524" aria-label="Article reference 7" data-doi="10.1021/es5046524">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 7" href="http://scholar.google.com/scholar_lookup?&title=Using%20natural%20abundance%20radiocarbon%20to%20trace%20the%20flux%20of%20petrocarbon%20to%20the%20seafloor%20following%20the%20Deepwater%20Horizon%20oil%20spill&journal=Environ%20Sci%20Technol&doi=10.1021%2Fes5046524&volume=49&pages=847-854&publication_year=2014&author=Chanton%2CJ&author=Zhao%2CT&author=Rosenheim%2CBE&author=Joye%2CS&author=Bosman%2CS&author=Brunner%2CC&author=Yeager%2CKM&author=Diercks%2CAR&author=Hollander%2CD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR8">Clarke KR, Green RH (1988) Statistical design and analysis for a ‘biological effects’ study. Mar Ecol Prog Ser 46:213–226</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3354/meps046213" data-track-item_id="10.3354/meps046213" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3354%2Fmeps046213" aria-label="Article reference 8" data-doi="10.3354/meps046213">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 8" href="http://scholar.google.com/scholar_lookup?&title=Statistical%20design%20and%20analysis%20for%20a%20%E2%80%98biological%20effects%E2%80%99%20study&journal=Mar%20Ecol%20Prog%20Ser&doi=10.3354%2Fmeps046213&volume=46&pages=213-226&publication_year=1988&author=Clarke%2CKR&author=Green%2CRH"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR9">Clarke KR, Gorley RN (2006) PRIMER v6: user manual/tutorial. PRIMER-E, Plymouth</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 9" href="http://scholar.google.com/scholar_lookup?&title=PRIMER%20v6%3A%20user%20manual%2Ftutorial&publication_year=2006&author=Clarke%2CKR&author=Gorley%2CRN"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR10">Cohen Y, Nissenbaum A, Eisler R (1977) Effects of Iranian crude oil on the Red Sea octocoral <i>Heteroxenia fuscescens</i>. Environ Pollut 12:173–186</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/0013-9327(77)90051-9" data-track-item_id="10.1016/0013-9327(77)90051-9" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2F0013-9327%2877%2990051-9" aria-label="Article reference 10" data-doi="10.1016/0013-9327(77)90051-9">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DyaE2sXhsFWitrY%3D" aria-label="CAS reference 10">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 10" href="http://scholar.google.com/scholar_lookup?&title=Effects%20of%20Iranian%20crude%20oil%20on%20the%20Red%20Sea%20octocoral%20Heteroxenia%20fuscescens&journal=Environ%20Pollut&doi=10.1016%2F0013-9327%2877%2990051-9&volume=12&pages=173-186&publication_year=1977&author=Cohen%2CY&author=Nissenbaum%2CA&author=Eisler%2CR"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR11">Coma R, Gili JM, Zabala M, Riera T (1994) Feeding and prey capture cycles in the aposymbiontic gorgonian <i>Paramuricea clavata</i>. Mar Ecol Prog Ser 115:257–270</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3354/meps115257" data-track-item_id="10.3354/meps115257" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3354%2Fmeps115257" aria-label="Article reference 11" data-doi="10.3354/meps115257">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 11" href="http://scholar.google.com/scholar_lookup?&title=Feeding%20and%20prey%20capture%20cycles%20in%20the%20aposymbiontic%20gorgonian%20Paramuricea%20clavata&journal=Mar%20Ecol%20Prog%20Ser&doi=10.3354%2Fmeps115257&volume=115&pages=257-270&publication_year=1994&author=Coma%2CR&author=Gili%2CJM&author=Zabala%2CM&author=Riera%2CT"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR12">Cooksey C, Hyland J, Fulton M, Balthis L, Wirth E, Wade T (2014) Ecological condition of coastal waters along the U.S. Continental Shelf of the Northeastern Gulf of Mexico: 2010. NOAA Technical Memorandum NOS NCCOS 188. NOAA National Ocean Service, Charleston</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 12" href="http://scholar.google.com/scholar_lookup?&title=Ecological%20condition%20of%20coastal%20waters%20along%20the%20U.S.%20Continental%20Shelf%20of%20the%20Northeastern%20Gulf%20of%20Mexico%3A%202010.%20NOAA%20Technical%20Memorandum%20NOS%20NCCOS%20188&publication_year=2014&author=Cooksey%2CC&author=Hyland%2CJ&author=Fulton%2CM&author=Balthis%2CL&author=Wirth%2CE&author=Wade%2CT"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR13">Davies AJ, Duineveld GC, Lavaleye MS, Bergman MJ, van Haren H, Roberts JM (2009) Downwelling and deep-water bottom currents as food supply mechanisms to the cold-water coral <i>Lophelia pertusa</i> (Scleractinia) at the Mingulay Reef complex. Limnol Oceanogr 54:620–629</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.4319/lo.2009.54.2.0620" data-track-item_id="10.4319/lo.2009.54.2.0620" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.4319%2Flo.2009.54.2.0620" aria-label="Article reference 13" data-doi="10.4319/lo.2009.54.2.0620">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 13" href="http://scholar.google.com/scholar_lookup?&title=Downwelling%20and%20deep-water%20bottom%20currents%20as%20food%20supply%20mechanisms%20to%20the%20cold-water%20coral%20Lophelia%20pertusa%20%28Scleractinia%29%20at%20the%20Mingulay%20Reef%20complex&journal=Limnol%20Oceanogr&doi=10.4319%2Flo.2009.54.2.0620&volume=54&pages=620-629&publication_year=2009&author=Davies%2CAJ&author=Duineveld%2CGC&author=Lavaleye%2CMS&author=Bergman%2CMJ&author=Haren%2CH&author=Roberts%2CJM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR14">DeLeo DM, Ruiz-Ramos DV, Baums IB, Cordes EE (2015) Response of deep-water corals to oil and chemical dispersant exposure. Deep Sea Res Part II Top Stud Oceanogr. doi:<a href="https://doi.org/10.1016/j.dsr2.2015.02.028" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="10.1016/j.dsr2.2015.02.028">10.1016/j.dsr2.2015.02.028</a> </p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 14" href="http://scholar.google.com/scholar_lookup?&title=Response%20of%20deep-water%20corals%20to%20oil%20and%20chemical%20dispersant%20exposure&journal=Deep%20Sea%20Res%20Part%20II%20Top%20Stud%20Oceanogr&doi=10.1016%2Fj.dsr2.2015.02.028&publication_year=2015&author=DeLeo%2CDM&author=Ruiz-Ramos%2CDV&author=Baums%2CIB&author=Cordes%2CEE"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR15">Eakin CM, Morgan JA, Heron SF, Smith TB, Liu G, Alvarez- Filip L, Baca B, Bartels E, Bastidas C, Bouchon C, Brandt M, Bruckner AW, Bunkley-Williams L, Cameron A, Causey B, Chiappone M, Christensen TRL, Crabbe MJC, Day O, de la Guardia E, Díaz-Pulido G, DiResta D, Gil-Agudelo D, Gilliam DS, Ginsburg RN, Gore S, Guzmán HM, Hendee JC, Hernández-Delgado EA, Husain E, Jeffrey CFG, Jones RJ, Jordán-Dahlgren E, Kaufman LS, Kline DI, Kramer PA, Lang JC, Lirman D, Mallela J, Manfrino C, Maréchal JP, Marks K, Mihaly J, Miller WJ, Mueller EM, Orozco Toro CA, Oxenford HA, Ponce-Taylor D, Quinn N, Ritchie KB, Rodríguez S, Rodríguez Ramírez A, Romano S, Samhouri JF, Sánchez JA, Schmahl GP, Shank BV, Skirving WJ, Steiner SCC, Villamizar E, Walsh SM, Walter C, Weil E, Williams EH, Woody Roberson K, Yusuf Y (2010) Caribbean corals in crisis: record thermal stress, bleaching, and mortality in 2005. PLoS One 5(11):e13969</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1371/journal.pone.0013969" data-track-item_id="10.1371/journal.pone.0013969" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1371%2Fjournal.pone.0013969" aria-label="Article reference 15" data-doi="10.1371/journal.pone.0013969">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2981599" aria-label="PubMed Central reference 15">PubMed Central</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=21125021" aria-label="PubMed reference 15">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 15" href="http://scholar.google.com/scholar_lookup?&title=Caribbean%20corals%20in%20crisis%3A%20record%20thermal%20stress%2C%20bleaching%2C%20and%20mortality%20in%202005&journal=PLoS%20One&doi=10.1371%2Fjournal.pone.0013969&volume=5&issue=11&publication_year=2010&author=Eakin%2CCM&author=Morgan%2CJA&author=Heron%2CSF&author=Smith%2CTB&author=Liu%2CG&author=Alvarez-%20Filip%2CL&author=Baca%2CB&author=Bartels%2CE&author=Bastidas%2CC&author=Bouchon%2CC&author=Brandt%2CM&author=Bruckner%2CAW&author=Bunkley-Williams%2CL&author=Cameron%2CA&author=Causey%2CB&author=Chiappone%2CM&author=Christensen%2CTRL&author=Crabbe%2CMJC&author=Day%2CO&author=Guardia%2CE&author=D%C3%ADaz-Pulido%2CG&author=DiResta%2CD&author=Gil-Agudelo%2CD&author=Gilliam%2CDS&author=Ginsburg%2CRN&author=Gore%2CS&author=Guzm%C3%A1n%2CHM&author=Hendee%2CJC&author=Hern%C3%A1ndez-Delgado%2CEA&author=Husain%2CE&author=Jeffrey%2CCFG&author=Jones%2CRJ&author=Jord%C3%A1n-Dahlgren%2CE&author=Kaufman%2CLS&author=Kline%2CDI&author=Kramer%2CPA&author=Lang%2CJC&author=Lirman%2CD&author=Mallela%2CJ&author=Manfrino%2CC&author=Mar%C3%A9chal%2CJP&author=Marks%2CK&author=Mihaly%2CJ&author=Miller%2CWJ&author=Mueller%2CEM&author=Orozco%20Toro%2CCA&author=Oxenford%2CHA&author=Ponce-Taylor%2CD&author=Quinn%2CN&author=Ritchie%2CKB&author=Rodr%C3%ADguez%2CS&author=Rodr%C3%ADguez%20Ram%C3%ADrez%2CA&author=Romano%2CS&author=Samhouri%2CJF&author=S%C3%A1nchez%2CJA&author=Schmahl%2CGP&author=Shank%2CBV&author=Skirving%2CWJ&author=Steiner%2CSCC&author=Villamizar%2CE&author=Walsh%2CSM&author=Walter%2CC&author=Weil%2CE&author=Williams%2CEH&author=Woody%20Roberson%2CK&author=Yusuf%2CY"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR16">Etnoyer P, Cairns SD, Sanchez JA, Reed JK, Lopez JV, Schroeder WW, Brooke SD, Watling L, Baco-Taylor A, Williams GC, Lindner A, France SC, Bruckner AW (2006) Deep-sea coral collection protocols. NOAA Technical Memorandum NMFS-OPR-28, Silver Spring</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 16" href="http://scholar.google.com/scholar_lookup?&title=Deep-sea%20coral%20collection%20protocols&publication_year=2006&author=Etnoyer%2CP&author=Cairns%2CSD&author=Sanchez%2CJA&author=Reed%2CJK&author=Lopez%2CJV&author=Schroeder%2CWW&author=Brooke%2CSD&author=Watling%2CL&author=Baco-Taylor%2CA&author=Williams%2CGC&author=Lindner%2CA&author=France%2CSC&author=Bruckner%2CAW"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR17">Fabricius KE, Genin A, Benayahu Y (1995) Flow-dependent herbivory and growth in zooxanthellae-free soft corals. Limnol Oceanogr 40:1290–1301</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.4319/lo.1995.40.7.1290" data-track-item_id="10.4319/lo.1995.40.7.1290" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.4319%2Flo.1995.40.7.1290" aria-label="Article reference 17" data-doi="10.4319/lo.1995.40.7.1290">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 17" href="http://scholar.google.com/scholar_lookup?&title=Flow-dependent%20herbivory%20and%20growth%20in%20zooxanthellae-free%20soft%20corals&journal=Limnol%20Oceanogr&doi=10.4319%2Flo.1995.40.7.1290&volume=40&pages=1290-1301&publication_year=1995&author=Fabricius%2CKE&author=Genin%2CA&author=Benayahu%2CY"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR18">Felder DL, Thoma BP, Schmidt WE, Sauvage T, Self-Krayesky SL, Chistoserdov A, Bracken-Grissom HD, Fredericq S (2014) Seaweeds and decapod crustaceans on Gulf deep banks after the Macondo oil spill. BioScience 64:808–819</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1093/biosci/biu119" data-track-item_id="10.1093/biosci/biu119" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1093%2Fbiosci%2Fbiu119" aria-label="Article reference 18" data-doi="10.1093/biosci/biu119">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 18" href="http://scholar.google.com/scholar_lookup?&title=Seaweeds%20and%20decapod%20crustaceans%20on%20Gulf%20deep%20banks%20after%20the%20Macondo%20oil%20spill&journal=BioScience&doi=10.1093%2Fbiosci%2Fbiu119&volume=64&pages=808-819&publication_year=2014&author=Felder%2CDL&author=Thoma%2CBP&author=Schmidt%2CWE&author=Sauvage%2CT&author=Self-Krayesky%2CSL&author=Chistoserdov%2CA&author=Bracken-Grissom%2CHD&author=Fredericq%2CS"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR19">Fisher CR, Demopoulos AWJ, Cordes EE, Baums IB, White HK, Bourque JR (2014) Coral communities as indicators of ecosystem-level impacts of the deepwater horizon spill. Bioscience 64:796–807</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1093/biosci/biu129" data-track-item_id="10.1093/biosci/biu129" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1093%2Fbiosci%2Fbiu129" aria-label="Article reference 19" data-doi="10.1093/biosci/biu129">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 19" href="http://scholar.google.com/scholar_lookup?&title=Coral%20communities%20as%20indicators%20of%20ecosystem-level%20impacts%20of%20the%20deepwater%20horizon%20spill&journal=Bioscience&doi=10.1093%2Fbiosci%2Fbiu129&volume=64&pages=796-807&publication_year=2014&author=Fisher%2CCR&author=Demopoulos%2CAWJ&author=Cordes%2CEE&author=Baums%2CIB&author=White%2CHK&author=Bourque%2CJR"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR20">Gardner JV, Sulak KJ, Dartnell P, Hellequin L, Calder B, Mayer LA (2000) Cruise report RV Ocean Surveyor Cruise O-1-00-GM the bathymetry and acoustic backscatter of the Pinnacles Area, Northern Gulf of Mexico May 23, through June 10, 2000. Venice, LA to Venice, LA. U.S. Geological Survey Open-File Report 00-350</p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR21">Gittings SR, Bright TJ, Schroeder WW, Sager WW, Laswell SJ, Rezak R (1992) Invertebrate assemblages and ecological controls on topographic features in the Northeast Gulf of Mexico. Bull Mar Sci 50:435–455</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 21" href="http://scholar.google.com/scholar_lookup?&title=Invertebrate%20assemblages%20and%20ecological%20controls%20on%20topographic%20features%20in%20the%20Northeast%20Gulf%20of%20Mexico&journal=Bull%20Mar%20Sci&volume=50&pages=435-455&publication_year=1992&author=Gittings%2CSR&author=Bright%2CTJ&author=Schroeder%2CWW&author=Sager%2CWW&author=Laswell%2CSJ&author=Rezak%2CR"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR22">Goodbody-Gringley G, Wetzel DL, Gillon D, Pulster E, Miller A, Ritchie KB (2013) Toxicity of deepwater horizon source oil and the chemical dispersant, Corexit<sup>®</sup> 9500, to coral larvae. PLoS One 8:e45574</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1371/journal.pone.0045574" data-track-item_id="10.1371/journal.pone.0045574" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1371%2Fjournal.pone.0045574" aria-label="Article reference 22" data-doi="10.1371/journal.pone.0045574">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541341" aria-label="PubMed Central reference 22">PubMed Central</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC3sXht1Smu7g%3D" aria-label="CAS reference 22">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=23326298" aria-label="PubMed reference 22">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 22" href="http://scholar.google.com/scholar_lookup?&title=Toxicity%20of%20deepwater%20horizon%20source%20oil%20and%20the%20chemical%20dispersant%2C%20Corexit%C2%AE%209500%2C%20to%20coral%20larvae&journal=PLoS%20One&doi=10.1371%2Fjournal.pone.0045574&volume=8&publication_year=2013&author=Goodbody-Gringley%2CG&author=Wetzel%2CDL&author=Gillon%2CD&author=Pulster%2CE&author=Miller%2CA&author=Ritchie%2CKB"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR23">Graham WM, Condon RH, Carmichael RH, D’Ambra I, Patterson HK, Linn LJ, Hernandez FJ Jr (2010) Oil carbon entered the coastal planktonic food web during the Deepwater Horizon oil spill. Environ Res Lett 5:e045301</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1088/1748-9326/5/4/045301" data-track-item_id="10.1088/1748-9326/5/4/045301" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1088%2F1748-9326%2F5%2F4%2F045301" aria-label="Article reference 23" data-doi="10.1088/1748-9326/5/4/045301">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 23" href="http://scholar.google.com/scholar_lookup?&title=Oil%20carbon%20entered%20the%20coastal%20planktonic%20food%20web%20during%20the%20Deepwater%20Horizon%20oil%20spill&journal=Environ%20Res%20Lett&doi=10.1088%2F1748-9326%2F5%2F4%2F045301&volume=5&publication_year=2010&author=Graham%2CWM&author=Condon%2CRH&author=Carmichael%2CRH&author=D%E2%80%99Ambra%2CI&author=Patterson%2CHK&author=Linn%2CLJ&author=Hernandez%2CFJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR24">Hall-Spencer JM, Pike J, Munn CB (2007) Diseases affect cold-water corals too: Eunicella verrucosa (Cnidaria: Gorgonacea) necrosis in SW England. Dis Aquat Org 76:87–97</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3354/dao076087" data-track-item_id="10.3354/dao076087" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3354%2Fdao076087" aria-label="Article reference 24" data-doi="10.3354/dao076087">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=17760382" aria-label="PubMed reference 24">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 24" href="http://scholar.google.com/scholar_lookup?&title=Diseases%20affect%20cold-water%20corals%20too%3A%20Eunicella%20verrucosa%20%28Cnidaria%3A%20Gorgonacea%29%20necrosis%20in%20SW%20England&journal=Dis%20Aquat%20Org&doi=10.3354%2Fdao076087&volume=76&pages=87-97&publication_year=2007&author=Hall-Spencer%2CJM&author=Pike%2CJ&author=Munn%2CCB"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR25">Hsing P-Y, Fu B, Larcom EA, Berlet SP, Shank TM, Govindarajan AF, Lukasiewicz AJ, Dixon PM, Fisher CR (2013) Evidence of lasting impact of the Deepwater Horizon oil spill on a deep Gulf of Mexico coral community. Elem Sci Anthr 1:0000012</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.12952/journal.elementa.000012" data-track-item_id="10.12952/journal.elementa.000012" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.12952%2Fjournal.elementa.000012" aria-label="Article reference 25" data-doi="10.12952/journal.elementa.000012">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 25" href="http://scholar.google.com/scholar_lookup?&title=Evidence%20of%20lasting%20impact%20of%20the%20Deepwater%20Horizon%20oil%20spill%20on%20a%20deep%20Gulf%20of%20Mexico%20coral%20community&journal=Elem%20Sci%20Anthr&doi=10.12952%2Fjournal.elementa.000012&volume=1&publication_year=2013&author=Hsing%2CP-Y&author=Fu%2CB&author=Larcom%2CEA&author=Berlet%2CSP&author=Shank%2CTM&author=Govindarajan%2CAF&author=Lukasiewicz%2CAJ&author=Dixon%2CPM&author=Fisher%2CCR"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR26">Johannes RE (1975) Pollution and degradation of coral reef communities. In: Johannes RE, Ferguson Wood EJ (eds) Tropical marine pollution. Elsevier, Amsterdam, pp 13–51</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/S0422-9894(08)71107-3" data-track-item_id="10.1016/S0422-9894(08)71107-3" data-track-value="chapter reference" data-track-action="chapter reference" href="https://doi.org/10.1016%2FS0422-9894%2808%2971107-3" aria-label="Chapter reference 26" data-doi="10.1016/S0422-9894(08)71107-3">Chapter</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 26" href="http://scholar.google.com/scholar_lookup?&title=Pollution%20and%20degradation%20of%20coral%20reef%20communities&doi=10.1016%2FS0422-9894%2808%2971107-3&pages=13-51&publication_year=1975&author=Johannes%2CRE"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR27">Kahng SE, Garcia-Sais JR, Spalding HL, Brokovich E, Wagner D, Weil E, Hinderstein L, Toonen RJ (2010) Community ecology of mesophotic coral reef systems. Coral Reefs 29:255–275</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="noopener" data-track-label="10.1007/s00338-010-0593-6" data-track-item_id="10.1007/s00338-010-0593-6" data-track-value="article reference" data-track-action="article reference" href="https://link.springer.com/doi/10.1007/s00338-010-0593-6" aria-label="Article reference 27" data-doi="10.1007/s00338-010-0593-6">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 27" href="http://scholar.google.com/scholar_lookup?&title=Community%20ecology%20of%20mesophotic%20coral%20reef%20systems&journal=Coral%20Reefs&doi=10.1007%2Fs00338-010-0593-6&volume=29&pages=255-275&publication_year=2010&author=Kahng%2CSE&author=Garcia-Sais%2CJR&author=Spalding%2CHL&author=Brokovich%2CE&author=Wagner%2CD&author=Weil%2CE&author=Hinderstein%2CL&author=Toonen%2CRJ"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR28">Kim K, Harvell D (2004) The rise and fall of a six year coral fungal epizootic. Am Nat 164(S5):S52–S63</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1086/424609" data-track-item_id="10.1086/424609" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1086%2F424609" aria-label="Article reference 28" data-doi="10.1086/424609">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=15540141" aria-label="PubMed reference 28">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 28" href="http://scholar.google.com/scholar_lookup?&title=The%20rise%20and%20fall%20of%20a%20six%20year%20coral%20fungal%20epizootic&journal=Am%20Nat&doi=10.1086%2F424609&volume=164&issue=S5&pages=S52-S63&publication_year=2004&author=Kim%2CK&author=Harvell%2CD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR29">Krieger KJ, Wing BL (2002) Megafauna associations with deepwater corals (<i>Primnoa</i> spp.) in the Gulf of Alaska. Hydrobiologia 471:83–90</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1023/A:1016597119297" data-track-item_id="10.1023/A:1016597119297" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1023%2FA%3A1016597119297" aria-label="Article reference 29" data-doi="10.1023/A:1016597119297">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 29" href="http://scholar.google.com/scholar_lookup?&title=Megafauna%20associations%20with%20deepwater%20corals%20%28Primnoa%20spp.%29%20in%20the%20Gulf%20of%20Alaska&journal=Hydrobiologia&doi=10.1023%2FA%3A1016597119297&volume=471&pages=83-90&publication_year=2002&author=Krieger%2CKJ&author=Wing%2CBL"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR30">Kushmora A, Henning G, Hofmann DK, Benayahu Y (1997) Metamorphosis of <i>Heteroxenia fuscescens</i> planulae (Cnidaria: Octocorallia) is inhibited by crude oil: a novel short term toxicity bioassay. Mar Environ Res 43:295–302</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/S0141-1136(96)00092-X" data-track-item_id="10.1016/S0141-1136(96)00092-X" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2FS0141-1136%2896%2900092-X" aria-label="Article reference 30" data-doi="10.1016/S0141-1136(96)00092-X">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 30" href="http://scholar.google.com/scholar_lookup?&title=Metamorphosis%20of%20Heteroxenia%20fuscescens%20planulae%20%28Cnidaria%3A%20Octocorallia%29%20is%20inhibited%20by%20crude%20oil%3A%20a%20novel%20short%20term%20toxicity%20bioassay&journal=Mar%20Environ%20Res&doi=10.1016%2FS0141-1136%2896%2900092-X&volume=43&pages=295-302&publication_year=1997&author=Kushmora%2CA&author=Henning%2CG&author=Hofmann%2CDK&author=Benayahu%2CY"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR31">Loya YM, Rinkevich B (1980) Effects of oil pollution on coral reef communities. Mar Ecol Prog Ser 3:167–180</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3354/meps003167" data-track-item_id="10.3354/meps003167" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3354%2Fmeps003167" aria-label="Article reference 31" data-doi="10.3354/meps003167">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 31" href="http://scholar.google.com/scholar_lookup?&title=Effects%20of%20oil%20pollution%20on%20coral%20reef%20communities&journal=Mar%20Ecol%20Prog%20Ser&doi=10.3354%2Fmeps003167&volume=3&pages=167-180&publication_year=1980&author=Loya%2CYM&author=Rinkevich%2CB"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR32">Mah C, Nizinski M, Lundsten L (2010) Phylogenetic revision of the Hippasterinae (Goniasteridae; Asteroidea): systematics of deep sea corallivores, including one new genus and three new species. Zool J Linn Soc 160:266–301</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1111/j.1096-3642.2010.00638.x" data-track-item_id="10.1111/j.1096-3642.2010.00638.x" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1111%2Fj.1096-3642.2010.00638.x" aria-label="Article reference 32" data-doi="10.1111/j.1096-3642.2010.00638.x">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 32" href="http://scholar.google.com/scholar_lookup?&title=Phylogenetic%20revision%20of%20the%20Hippasterinae%20%28Goniasteridae%3B%20Asteroidea%29%3A%20systematics%20of%20deep%20sea%20corallivores%2C%20including%20one%20new%20genus%20and%20three%20new%20species&journal=Zool%20J%20Linn%20Soc&doi=10.1111%2Fj.1096-3642.2010.00638.x&volume=160&pages=266-301&publication_year=2010&author=Mah%2CC&author=Nizinski%2CM&author=Lundsten%2CL"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR33">McNutt MK, Camilli R, Crone TJ, Guthrie GD, Hsieh PA, Ryerson TB, Savas O, Shaffer F (2011) Review of flow rate estimates of the Deepwater Horizon oil spill. Proc Natl Acad Sci USA 109:20260–20267</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1073/pnas.1112139108" data-track-item_id="10.1073/pnas.1112139108" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1073%2Fpnas.1112139108" aria-label="Article reference 33" data-doi="10.1073/pnas.1112139108">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528583" aria-label="PubMed Central reference 33">PubMed Central</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=22187459" aria-label="PubMed reference 33">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 33" href="http://scholar.google.com/scholar_lookup?&title=Review%20of%20flow%20rate%20estimates%20of%20the%20Deepwater%20Horizon%20oil%20spill&journal=Proc%20Natl%20Acad%20Sci%20USA&doi=10.1073%2Fpnas.1112139108&volume=109&pages=20260-20267&publication_year=2011&author=McNutt%2CMK&author=Camilli%2CR&author=Crone%2CTJ&author=Guthrie%2CGD&author=Hsieh%2CPA&author=Ryerson%2CTB&author=Savas%2CO&author=Shaffer%2CF"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR34">Montagna PA, Baguley JG, Cooksey C, Hartwell I, Hyde LJ, Hyland JL, Kalke RD, Kracker LM, Reuscher M, Rhodes ACE (2013) Deep-sea benthic footprint of the Deepwater Horizon blowout. PLoS One 8:e70540</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1371/journal.pone.0070540" data-track-item_id="10.1371/journal.pone.0070540" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1371%2Fjournal.pone.0070540" aria-label="Article reference 34" data-doi="10.1371/journal.pone.0070540">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3737147" aria-label="PubMed Central reference 34">PubMed Central</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC3sXhtlWgtL7O" aria-label="CAS reference 34">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=23950956" aria-label="PubMed reference 34">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 34" href="http://scholar.google.com/scholar_lookup?&title=Deep-sea%20benthic%20footprint%20of%20the%20Deepwater%20Horizon%20blowout&journal=PLoS%20One&doi=10.1371%2Fjournal.pone.0070540&volume=8&publication_year=2013&author=Montagna%2CPA&author=Baguley%2CJG&author=Cooksey%2CC&author=Hartwell%2CI&author=Hyde%2CLJ&author=Hyland%2CJL&author=Kalke%2CRD&author=Kracker%2CLM&author=Reuscher%2CM&author=Rhodes%2CACE"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR35">Murawski SA, Hogarth WT, Peebles EB, Barbeiri L (2014) Prevalence of external skin lesions and polycyclic aromatic hydrocarbon concentrations in Gulf of Mexico fishes, post-Deepwater Horizon. Trans Am Fish Soc 143:1084–1097</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2cXhtFeru7zJ" aria-label="CAS reference 35">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 35" href="http://scholar.google.com/scholar_lookup?&title=Prevalence%20of%20external%20skin%20lesions%20and%20polycyclic%20aromatic%20hydrocarbon%20concentrations%20in%20Gulf%20of%20Mexico%20fishes%2C%20post-Deepwater%20Horizon&journal=Trans%20Am%20Fish%20Soc&volume=143&pages=1084-1097&publication_year=2014&author=Murawski%2CSA&author=Hogarth%2CWT&author=Peebles%2CEB&author=Barbeiri%2CL"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR36">Nash UA and Randall M (2015) GIS determination of the area of high-profile (>2 m elevation) reef-top biotope within the Pinnacles polygon- based on USGS high resolution multibeam sonar acoustic backscatter data acquired in 2000. USGS Coastal Ecology and Conservation Research Group, Gainesville, FL. Internal Briefing to NRDA TWG, 21 April 2015. 23 pp</p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR37">NOAA (2010) Oil spills in coral reefs: planning and response considerations. In: Yender RA, Michel J (eds) US Department of Commerce, National Oceanic and Atmospheric Administration. Silver Spring, MD, pp 2–82</p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR38">NOAA (2014) Cumulative TCNNA SAR Oiling - Days of Oiling, <i>Environmental Response Management Application</i>. Web application. ERMA Deepwater Horizon MC252. National Oceanic and Atmospheric Administration. Accessed 30 Jan 2015. <a href="http://response.restoration.noaa.gov/erma/" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="http://response.restoration.noaa.gov/erma/">http://response.restoration.noaa.gov/erma/</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR39">Passow U, Ziervogel K, Asper V, Diercks A (2012) Marine snow formation in the aftermath of the Deepwater Horizon oil spill in the Gulf of Mexico. Environ Res Lett 7:035301</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1088/1748-9326/7/3/035301" data-track-item_id="10.1088/1748-9326/7/3/035301" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1088%2F1748-9326%2F7%2F3%2F035301" aria-label="Article reference 39" data-doi="10.1088/1748-9326/7/3/035301">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 39" href="http://scholar.google.com/scholar_lookup?&title=Marine%20snow%20formation%20in%20the%20aftermath%20of%20the%20Deepwater%20Horizon%20oil%20spill%20in%20the%20Gulf%20of%20Mexico&journal=Environ%20Res%20Lett&doi=10.1088%2F1748-9326%2F7%2F3%2F035301&volume=7&publication_year=2012&author=Passow%2CU&author=Ziervogel%2CK&author=Asper%2CV&author=Diercks%2CA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR40">Peccini MB, MacDonald IR (2008) Correspondence of sea fan orientations with measured currents on hard bottom habitats of the Mississippi/Alabama continental shelf. Cont Shelf Res 28:302–308</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.csr.2007.08.003" data-track-item_id="10.1016/j.csr.2007.08.003" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2Fj.csr.2007.08.003" aria-label="Article reference 40" data-doi="10.1016/j.csr.2007.08.003">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 40" href="http://scholar.google.com/scholar_lookup?&title=Correspondence%20of%20sea%20fan%20orientations%20with%20measured%20currents%20on%20hard%20bottom%20habitats%20of%20the%20Mississippi%2FAlabama%20continental%20shelf&journal=Cont%20Shelf%20Res&doi=10.1016%2Fj.csr.2007.08.003&volume=28&pages=302-308&publication_year=2008&author=Peccini%2CMB&author=MacDonald%2CIR"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR41">Peters EC, Meyers P, Yevich P, Blake N (1981) Bioaccumulation and histopathological effects of oil on a stony coral. Mar Pollut Bull 12:333–339</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/0025-326X(81)90106-5" data-track-item_id="10.1016/0025-326X(81)90106-5" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2F0025-326X%2881%2990106-5" aria-label="Article reference 41" data-doi="10.1016/0025-326X(81)90106-5">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DyaL3MXmtFKjsbw%3D" aria-label="CAS reference 41">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 41" href="http://scholar.google.com/scholar_lookup?&title=Bioaccumulation%20and%20histopathological%20effects%20of%20oil%20on%20a%20stony%20coral&journal=Mar%20Pollut%20Bull&doi=10.1016%2F0025-326X%2881%2990106-5&volume=12&pages=333-339&publication_year=1981&author=Peters%2CEC&author=Meyers%2CP&author=Yevich%2CP&author=Blake%2CN"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR42">Prytherch HF (1983) A descriptive survey of the bottom longline fishery in the Gulf of Mexico. NOAA Technical Memorandum NMFS-SEFC-122:1–33</p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR43">Rezak R, Bright TJ, McGrail W (1985) Reefs and Banks of the Northwestern Gulf of Mexico: their geological, biological, and physical dynamics. John Wiley and Sons, New York</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 43" href="http://scholar.google.com/scholar_lookup?&title=Reefs%20and%20Banks%20of%20the%20Northwestern%20Gulf%20of%20Mexico%3A%20their%20geological%2C%20biological%2C%20and%20physical%20dynamics&publication_year=1985&author=Rezak%2CR&author=Bright%2CTJ&author=McGrail%2CW"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR44">Ribes M, Coma R, Gili JM (1999) Heterogeneous feeding in benthic suspension feeders: the natural diet and grazing rate of the temperate gorgonian <i>Paramuricea clavata</i> (Cnidaria; Octocorallia) over a year cycle. Mar Ecol Prog Ser 183:125–137</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3354/meps183125" data-track-item_id="10.3354/meps183125" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3354%2Fmeps183125" aria-label="Article reference 44" data-doi="10.3354/meps183125">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 44" href="http://scholar.google.com/scholar_lookup?&title=Heterogeneous%20feeding%20in%20benthic%20suspension%20feeders%3A%20the%20natural%20diet%20and%20grazing%20rate%20of%20the%20temperate%20gorgonian%20Paramuricea%20clavata%20%28Cnidaria%3B%20Octocorallia%29%20over%20a%20year%20cycle&journal=Mar%20Ecol%20Prog%20Ser&doi=10.3354%2Fmeps183125&volume=183&pages=125-137&publication_year=1999&author=Ribes%2CM&author=Coma%2CR&author=Gili%2CJM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR45">Sampaio I, Braga-Henriques A, Pham C, Ocana O, De Matos V, Morato T, Porteiro FM (2012) Cold-water corals landed by bottom longline fisheries in the Azores (north-eastern Atlantic). J Mar Biol Assoc UK 92:1547–1555</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1017/S0025315412000045" data-track-item_id="10.1017/S0025315412000045" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1017%2FS0025315412000045" aria-label="Article reference 45" data-doi="10.1017/S0025315412000045">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 45" href="http://scholar.google.com/scholar_lookup?&title=Cold-water%20corals%20landed%20by%20bottom%20longline%20fisheries%20in%20the%20Azores%20%28north-eastern%20Atlantic%29&journal=J%20Mar%20Biol%20Assoc%20UK&doi=10.1017%2FS0025315412000045&volume=92&pages=1547-1555&publication_year=2012&author=Sampaio%2CI&author=Braga-Henriques%2CA&author=Pham%2CC&author=Ocana%2CO&author=Matos%2CV&author=Morato%2CT&author=Porteiro%2CFM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR46">Sánchez JA, Ardila NE, Andrade J, Dueñas LF, Navas R, Ballesteros D (2014) Octocoral densities and mortalities in Gorgona Island, Colombia, Tropical Eastern Pacific. Int J Trop Biol 62:209–219</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 46" href="http://scholar.google.com/scholar_lookup?&title=Octocoral%20densities%20and%20mortalities%20in%20Gorgona%20Island%2C%20Colombia%2C%20Tropical%20Eastern%20Pacific&journal=Int%20J%20Trop%20Biol&volume=62&pages=209-219&publication_year=2014&author=S%C3%A1nchez%2CJA&author=Ardila%2CNE&author=Andrade%2CJ&author=Due%C3%B1as%2CLF&author=Navas%2CR&author=Ballesteros%2CD"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR47">Scott-Denton E, Cryer PF, Gocke JP, Harrelson MR, Kinsella DL, Pulver JR, Smith RC, Williams JA (2011) Descriptions of the U.S. Gulf of Mexico reef fish bottom longline and vertical line fisheries based on observer data. Mar Fish Rev 72:1–26</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 47" href="http://scholar.google.com/scholar_lookup?&title=Descriptions%20of%20the%20U.S.%20Gulf%20of%20Mexico%20reef%20fish%20bottom%20longline%20and%20vertical%20line%20fisheries%20based%20on%20observer%20data&journal=Mar%20Fish%20Rev&volume=72&pages=1-26&publication_year=2011&author=Scott-Denton%2CE&author=Cryer%2CPF&author=Gocke%2CJP&author=Harrelson%2CMR&author=Kinsella%2CDL&author=Pulver%2CJR&author=Smith%2CRC&author=Williams%2CJA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR48">Sherwood OA, Heikoop JM, Scott DB, Risk MJ, Guilderson TP, McKinney RA (2005) Stable isotopic composition of deep-sea gorgonian corals <i>Primnoa</i> spp.: a new archive of surface processes. Mar Ecol Prog Ser 301:135–148</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.3354/meps301135" data-track-item_id="10.3354/meps301135" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.3354%2Fmeps301135" aria-label="Article reference 48" data-doi="10.3354/meps301135">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BD28Xks1GqtQ%3D%3D" aria-label="CAS reference 48">CAS</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 48" href="http://scholar.google.com/scholar_lookup?&title=Stable%20isotopic%20composition%20of%20deep-sea%20gorgonian%20corals%20Primnoa%20spp.%3A%20a%20new%20archive%20of%20surface%20processes&journal=Mar%20Ecol%20Prog%20Ser&doi=10.3354%2Fmeps301135&volume=301&pages=135-148&publication_year=2005&author=Sherwood%2COA&author=Heikoop%2CJM&author=Scott%2CDB&author=Risk%2CMJ&author=Guilderson%2CTP&author=McKinney%2CRA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR49">Silva M, Etnoyer PJ, MacDonald IR (2015) Coral injuries observed at mesophotic reefs after the Deepwater Horizon oil discharge. Deep Sea Res Part II Top Stud Oceanogr. doi:<a href="https://doi.org/10.1016/j.dsr2.2015.05.013" data-track="click_references" data-track-action="external reference" data-track-value="external reference" data-track-label="10.1016/j.dsr2.2015.05.013">10.1016/j.dsr2.2015.05.013</a> </p><p class="c-article-references__links u-hide-print"><a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 49" href="http://scholar.google.com/scholar_lookup?&title=Coral%20injuries%20observed%20at%20mesophotic%20reefs%20after%20the%20Deepwater%20Horizon%20oil%20discharge&journal=Deep%20Sea%20Res%20Part%20II%20Top%20Stud%20Oceanogr&doi=10.1016%2Fj.dsr2.2015.05.013&publication_year=2015&author=Silva%2CM&author=Etnoyer%2CPJ&author=MacDonald%2CIR"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR50">Sulak K, Berg J, Randall M, Dennis III GD, Brooks RA (2008) Dual-carbon sources fuel the OCS deep-reef community, a stable isotope investigation. Proc 11th Int Coral Reef Symp 2:945–949</p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR51">Trygonis V, Sini M (2012) photoQuad: a dedicated seabed image processing software, and a comparative error analysis of four photoquadrat methods. J Exp Mar Biol Ecol 424:99–108</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1016/j.jembe.2012.04.018" data-track-item_id="10.1016/j.jembe.2012.04.018" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1016%2Fj.jembe.2012.04.018" aria-label="Article reference 51" data-doi="10.1016/j.jembe.2012.04.018">Article</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 51" href="http://scholar.google.com/scholar_lookup?&title=photoQuad%3A%20a%20dedicated%20seabed%20image%20processing%20software%2C%20and%20a%20comparative%20error%20analysis%20of%20four%20photoquadrat%20methods&journal=J%20Exp%20Mar%20Biol%20Ecol&doi=10.1016%2Fj.jembe.2012.04.018&volume=424&pages=99-108&publication_year=2012&author=Trygonis%2CV&author=Sini%2CM"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR52">Valentine DL, Fisher GB, Nelson RK, Reddy CM, Sylva SP, Woo MA (2014) Fallout plume of submerged oil from Deepwater Horizon. Proc Natl Acad Sci USA 111:15906–15911</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1073/pnas.1414873111" data-track-item_id="10.1073/pnas.1414873111" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1073%2Fpnas.1414873111" aria-label="Article reference 52" data-doi="10.1073/pnas.1414873111">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4234598" aria-label="PubMed Central reference 52">PubMed Central</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC2cXhvVWmsL3M" aria-label="CAS reference 52">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=25349409" aria-label="PubMed reference 52">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 52" href="http://scholar.google.com/scholar_lookup?&title=Fallout%20plume%20of%20submerged%20oil%20from%20Deepwater%20Horizon&journal=Proc%20Natl%20Acad%20Sci%20USA&doi=10.1073%2Fpnas.1414873111&volume=111&pages=15906-15911&publication_year=2014&author=Valentine%2CDL&author=Fisher%2CGB&author=Nelson%2CRK&author=Reddy%2CCM&author=Sylva%2CSP&author=Woo%2CMA"> Google Scholar</a> </p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR53">Weaver DC, Dennis GD, Sulak KJ (2002) Community structure and trophic ecology of demersal fishes on the Pinnacles Reef tract. USGS BSR 2001–2008</p></li><li class="c-article-references__item js-c-reading-companion-references-item"><p class="c-article-references__text" id="ref-CR54">White HK, Hsing PY, Cho W, Shank TM, Cordes EE, Quattrini AM, Nelson RK, Camilli R, Demopoulos AWJ, German CR, Brooks JM, Roberts HH, Shedd W, Reddy CM, Fisher CR (2012) Impact of the Deepwater Horizon oil spill on a deep-water coral community in the Gulf of Mexico. Proc Natl Acad Sci USA 109:20303–22030</p><p class="c-article-references__links u-hide-print"><a data-track="click_references" rel="nofollow noopener" data-track-label="10.1073/pnas.1118029109" data-track-item_id="10.1073/pnas.1118029109" data-track-value="article reference" data-track-action="article reference" href="https://doi.org/10.1073%2Fpnas.1118029109" aria-label="Article reference 54" data-doi="10.1073/pnas.1118029109">Article</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed central reference" data-track-action="pubmed central reference" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3528508" aria-label="PubMed Central reference 54">PubMed Central</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="cas reference" data-track-action="cas reference" href="/articles/cas-redirect/1:CAS:528:DC%2BC3sXnvVSntA%3D%3D" aria-label="CAS reference 54">CAS</a> <a data-track="click_references" rel="nofollow noopener" data-track-label="link" data-track-item_id="link" data-track-value="pubmed reference" data-track-action="pubmed reference" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=22454495" aria-label="PubMed reference 54">PubMed</a> <a data-track="click_references" data-track-action="google scholar reference" data-track-value="google scholar reference" data-track-label="link" data-track-item_id="link" rel="nofollow noopener" aria-label="Google Scholar reference 54" href="http://scholar.google.com/scholar_lookup?&title=Impact%20of%20the%20Deepwater%20Horizon%20oil%20spill%20on%20a%20deep-water%20coral%20community%20in%20the%20Gulf%20of%20Mexico&journal=Proc%20Natl%20Acad%20Sci%20USA&doi=10.1073%2Fpnas.1118029109&volume=109&pages=20303-22030&publication_year=2012&author=White%2CHK&author=Hsing%2CPY&author=Cho%2CW&author=Shank%2CTM&author=Cordes%2CEE&author=Quattrini%2CAM&author=Nelson%2CRK&author=Camilli%2CR&author=Demopoulos%2CAWJ&author=German%2CCR&author=Brooks%2CJM&author=Roberts%2CHH&author=Shedd%2CW&author=Reddy%2CCM&author=Fisher%2CCR"> Google Scholar</a> </p></li></ul><p class="c-article-references__download u-hide-print"><a data-track="click" data-track-action="download citation references" data-track-label="link" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1007/s00338-015-1363-2?format=refman&flavour=references">Download references<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-download-medium"></use></svg></a></p></div></div></div></section></div><section data-title="Acknowledgments"><div class="c-article-section" id="Ack1-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="Ack1">Acknowledgments</h2><div class="c-article-section__content" id="Ack1-content"><p>The authors would like to acknowledge support from the DWH NRDA since 2010. We would also like to acknowledge the efforts of USGS since 1997 under the leadership of Dr. Ken Sulak. The captain and crews of NOAA ship Nancy Foster, RV Holiday Chouest, and RV Walton Smith were indispensable, as were the efforts of ROV teams from Deep Sea Systems International and Schilling Robotics. We appreciate A. Shuler, M. Rittinghouse, E. Couch, J. Frometa, and G. Hanna for their significant contributions. A. Shuler prepared the species identification guide in ESM. Special thanks to SD Cairns for help with species identification.</p></div></div></section><section aria-labelledby="author-information" data-title="Author information"><div class="c-article-section" id="author-information-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="author-information">Author information</h2><div class="c-article-section__content" id="author-information-content"><h3 class="c-article__sub-heading" id="affiliations">Authors and Affiliations</h3><ol class="c-article-author-affiliation__list"><li id="Aff1"><p class="c-article-author-affiliation__address">NOAA Center for Coastal Environmental Health and Biomolecular Research, 219 Fort Johnson Rd., Charleston, SC, 29412, USA</p><p class="c-article-author-affiliation__authors-list">Peter J. Etnoyer & Len Balthis</p></li><li id="Aff2"><p class="c-article-author-affiliation__address">JHT, Inc., 2710 Discovery Dr., Suite 100, Orlando, FL, 32826, USA</p><p class="c-article-author-affiliation__authors-list">Leslie N. Wickes, J. D. Dubick & Enrique Salgado</p></li><li id="Aff3"><p class="c-article-author-affiliation__address">Florida State University, Tallahassee, FL, 32306, USA</p><p class="c-article-author-affiliation__authors-list">Mauricio Silva & Ian R. MacDonald</p></li></ol><div class="u-js-hide u-hide-print" data-test="author-info"><span class="c-article__sub-heading">Authors</span><ol class="c-article-authors-search u-list-reset"><li id="auth-Peter_J_-Etnoyer-Aff1"><span class="c-article-authors-search__title u-h3 js-search-name">Peter J. Etnoyer</span><div class="c-article-authors-search__list"><div class="c-article-authors-search__item c-article-authors-search__list-item--left"><a href="/search?dc.creator=Peter%20J.%20Etnoyer" class="c-article-button" data-track="click" data-track-action="author link - publication" data-track-label="link" rel="nofollow">View author publications</a></div><div class="c-article-authors-search__item c-article-authors-search__list-item--right"><p class="search-in-title-js c-article-authors-search__text">You can also search for this author in <span class="c-article-identifiers"><a class="c-article-identifiers__item" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&term=Peter%20J.%20Etnoyer" data-track="click" data-track-action="author link - pubmed" data-track-label="link" rel="nofollow">PubMed</a><span class="u-hide"> </span><a class="c-article-identifiers__item" href="http://scholar.google.co.uk/scholar?as_q=&num=10&btnG=Search+Scholar&as_epq=&as_oq=&as_eq=&as_occt=any&as_sauthors=%22Peter%20J.%20Etnoyer%22&as_publication=&as_ylo=&as_yhi=&as_allsubj=all&hl=en" data-track="click" data-track-action="author link - scholar" data-track-label="link" rel="nofollow">Google Scholar</a></span></p></div></div></li><li id="auth-Leslie_N_-Wickes-Aff2"><span class="c-article-authors-search__title u-h3 js-search-name">Leslie N. 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Etnoyer</a>.</p></div></div></section><section data-title="Ethics declarations"><div class="c-article-section" id="ethics-section"><h2 class="c-article-section__title js-section-title js-c-reading-companion-sections-item" id="ethics">Ethics declarations</h2><div class="c-article-section__content" id="ethics-content"> <h3 class="c-article__sub-heading" id="FPar1">NOAA Disclaimer</h3> <p>This publication does not constitute an endorsement of any commercial product or intend to be an opinion beyond scientific or other results obtained by the National Oceanic and Atmospheric Administration (NOAA). 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id="citeas">Cite this article</h3><p class="c-bibliographic-information__citation">Etnoyer, P.J., Wickes, L.N., Silva, M. <i>et al.</i> Decline in condition of gorgonian octocorals on mesophotic reefs in the northern Gulf of Mexico: before and after the Deepwater Horizon oil spill. <i>Coral Reefs</i> <b>35</b>, 77–90 (2016). https://doi.org/10.1007/s00338-015-1363-2</p><p class="c-bibliographic-information__download-citation u-hide-print"><a data-test="citation-link" data-track="click" data-track-action="download article citation" data-track-label="link" data-track-external="" rel="nofollow" href="https://citation-needed.springer.com/v2/references/10.1007/s00338-015-1363-2?format=refman&flavour=citation">Download citation<svg width="16" height="16" focusable="false" role="img" aria-hidden="true" class="u-icon"><use xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="#icon-eds-i-download-medium"></use></svg></a></p><ul class="c-bibliographic-information__list" data-test="publication-history"><li class="c-bibliographic-information__list-item"><p>Received<span class="u-hide">: </span><span class="c-bibliographic-information__value"><time datetime="2015-02-06">06 February 2015</time></span></p></li><li class="c-bibliographic-information__list-item"><p>Accepted<span class="u-hide">: </span><span class="c-bibliographic-information__value"><time datetime="2015-10-05">05 October 2015</time></span></p></li><li class="c-bibliographic-information__list-item"><p>Published<span class="u-hide">: </span><span class="c-bibliographic-information__value"><time datetime="2015-10-20">20 October 2015</time></span></p></li><li class="c-bibliographic-information__list-item"><p>Issue Date<span class="u-hide">: </span><span class="c-bibliographic-information__value"><time datetime="2016-03">March 2016</time></span></p></li><li class="c-bibliographic-information__list-item c-bibliographic-information__list-item--full-width"><p><abbr title="Digital Object 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