How to Perform a TUNEL Assay?

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First, cells or tissue sections are fixed and permeabilized to allow access to the DNA. Next, TdT enzyme and labeled dUTPs are added to the samples. After incubation, the samples are washed, and the labeled DNA fragments are visualized using fluorescence or colorimetric detection. It is crucial to include positive and negative controls to validate the assay results.<br></div></div> </div> <hr /> <h3 class="rq-heading">Frequently asked queries:</h3> <div class="qa-container"> <div class="qa-item"> <a href="https://cellbiology.blog/knowledgebase/what-is-a-tunel-assay" title="What is a TUNEL Assay?">What is a TUNEL Assay?</a> </div> <div class="qa-item"> <a href="https://cellbiology.blog/knowledgebase/how-does-the-tunel-assay-work" title="How Does the TUNEL Assay Work?">How Does the TUNEL Assay Work?</a> </div> <div class="qa-item"> <a href="https://cellbiology.blog/knowledgebase/how-to-perform-a-tunel-assay" title="How to Perform a TUNEL Assay?">How to Perform a TUNEL Assay?</a> </div> <div class="qa-item"> <a href="https://cellbiology.blog/knowledgebase/what-role-do-organelles-play" title="What Role Do Organelles Play?">What Role Do Organelles Play?</a> </div> <div class="qa-item"> <a href="https://cellbiology.blog/knowledgebase/what-are-carcinogens" title="What 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