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Topics</h1> </div> <div class="module-buttons"> <span class="toggle-button module-button-item collapse" title="Collapse" data-toggle-title="Expand"><span class="b-icon b-icon__toggle-collapse">Collapse</span></span> </div> </div> <hr class="widget-header-divider" /> <div class="widget-content"> <ul class="conversation-list list-container h-clearfix"> <li class="b-post-sm js-post-sm" data-nodeid="326290"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/149680-novogene"> <img src="./core/image.php?userid=149680&thumb=1&dateline=1716924809" width="32" height="32" alt="Novogene" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/vendor-forum/326290-amplicon-vs-shotgun-metagenomics-–-how-do-i-choose">Amplicon vs Shotgun Metagenomics – How Do I Choose?</a> </div> <div class="b-post-sm__author"> by <a href="https://www.seqanswers.com/member/149680-novogene" data-vbnamecard="149680">Novogene</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> Metagenomics is the study of microbial communities in their original habitats and gives a comprehensive insight into the biochemical and metabolic interactions... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326290"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/vendor-forum">Sequencing Resources</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">11-21-2024, 02:02 PM</div> </div> </li> <li class="b-post-sm js-post-sm" data-nodeid="326282"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/469-dsturgill"> <img src="./core/images/default/default_avatar_thumb.png" width="32" height="32" alt="dsturgill" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/forum/bioinformatics/bioinformatics-aa/326282-gdc-analysis-tool-challenge">GDC Analysis Tool Challenge</a> </div> <div class="b-post-sm__author"> by <a href="https://www.seqanswers.com/member/469-dsturgill" data-vbnamecard="469">dsturgill</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> Here is a announcement for the GDC Analysis Tool Challenge, with a registration deadline of 11/25/2024. Follow the link to the challenge site to register.... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326282"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/forum/bioinformatics/bioinformatics-aa">Bioinformatics</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">11-18-2024, 02:05 PM</div> </div> </li> <li class="b-post-sm js-post-sm" data-nodeid="326279"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/150365-césar"> <img src="./core/images/default/default_avatar_thumb.png" width="32" height="32" alt="César" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/forum/applications-forums/rna-sequencing/326279-how-to-make-a-heatmap-with-the-exons-of-a-gene-with-bam-file-of-rna-seq-in-r-studio">How to make a heatmap with the exons of a gene with bam file of RNA-seq in R-studio?</a> </div> <div class="b-post-sm__author"> by <a href="https://www.seqanswers.com/member/150365-césar" data-vbnamecard="150365">César</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> Hi, I´m new in this kind of analysis in RNA-seq but I´m trying to make a heatmap with the exons of a gene. My files are from a public database and first... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326279"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/forum/applications-forums/rna-sequencing">RNA Sequencing</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">11-14-2024, 10:26 PM</div> </div> </li> </ul> <div class="widget-footer h-align-left h-clear"> <a href="https://www.seqanswers.com/search?r=78064133" class="b-button">View All</a> </div> </div> </div>   <div class="b-module canvas-widget default-widget activity-stream-widget forum-activity-stream-widget widget-tabs ui-tabs widget-no-header-buttons widget-no-border" id="widget_3" data-widget-id="6" data-widget-instance-id="3" data-widget-default-tab="#forum-tab"> <div class="widget-header widget-tabs-nav h-clearfix"> <div class="module-title h-left"> <ul class="ui-tabs-nav hide-on-editmode h-clearfix 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multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer nucleotide complexes bound to clonal copies of DNA targets.</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=323994" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 2 Posts: 5 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/element-biosciences/325771-weird-16s-custom-primer-issue-on-aviti-runs?p=325812#post325812" class="lastpost-title">Weird 16S custom primer issue on Aviti runs</a> </div> </td> <td class="topics-count">2</td> <td class="posts-count">5</td> <td class="lastpost"> <div class="lastpost-wrapper"> <a href="https://www.seqanswers.com/member/38168-iddan" class="avatar h-left"> <img src="./core/images/default/default_avatar_thumb.png" alt="IDDan" title="IDDan" /> </a> <div class="lastpost-info"> <div class="lastpost-title-wrapper ellipsis"> <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/element-biosciences/325771-weird-16s-custom-primer-issue-on-aviti-runs?p=325812#post325812" class="lastpost-title">Weird 16S custom primer issue on Aviti runs</a> </div> <div class="lastpost-by"> by <a href="https://www.seqanswers.com/member/38168-iddan" data-vbnamecard="38168">IDDan</a> </div> <div class="lastpost-date post-date">06-12-2024, 10:46 AM</div> </div> </div> </td> </tr>  <tr class="forum-item main new" id="forum29" data-channel-id="29" data-lastcontent="1725309447" data-readtime="0"> <td class="cell-forum"> <div class="forum-wrapper"> <div class="forum-info forum-info-has-rss"> <span class="icon b-icon b-icon__speech-double-large--blue h-left" title="Contains unread posts." ></span> <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/illumina-solexa" class="forum-title">Illumina (Solexa)</a> <div class="forum-desc">Bridged amplification & clustering followed by sequencing by synthesis.</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=29" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 3,057 Posts: 16,723 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/illumina-solexa/326036-re-basecalling-illumina-data-to-improve-accuracy#post326036" class="lastpost-title">Re-basecalling Illumina data to improve accuracy?</a> </div> </td> <td class="topics-count">3,057</td> <td class="posts-count">16,723</td> <td class="lastpost"> <div class="lastpost-wrapper"> <a href="https://www.seqanswers.com/member/149114-hjarnek" class="avatar h-left"> <img src="./core/images/default/default_avatar_thumb.png" alt="hjarnek" title="hjarnek" /> </a> <div class="lastpost-info"> <div class="lastpost-title-wrapper ellipsis"> <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/illumina-solexa/326036-re-basecalling-illumina-data-to-improve-accuracy#post326036" class="lastpost-title">Re-basecalling Illumina data to improve accuracy?</a> </div> <div class="lastpost-by"> by <a href="https://www.seqanswers.com/member/149114-hjarnek" data-vbnamecard="149114">hjarnek</a> </div> <div class="lastpost-date post-date">09-02-2024, 12:37 PM</div> </div> </div> </td> </tr>  <tr class="forum-item main new" id="forum52" data-channel-id="52" data-lastcontent="1715289567" data-readtime="0"> <td class="cell-forum"> <div class="forum-wrapper"> <div class="forum-info forum-info-has-rss"> <span class="icon b-icon b-icon__speech-double-large--blue h-left" title="Contains unread posts." ></span> <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/ion-torrent" class="forum-title">Ion Torrent</a> <div class="forum-desc">Integrated electronic detection of protons released upon base incorporation. (Thermo Fisher Scientific)</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=52" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 392 Posts: 2,174 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/ion-torrent/325723-ion-ampliseq-library-prep-query#post325723" class="lastpost-title">Ion Ampliseq library prep query</a> </div> </td> <td class="topics-count">392</td> <td class="posts-count">2,174</td> <td class="lastpost"> <div class="lastpost-wrapper"> <a href="https://www.seqanswers.com/member/149598-monalinm" class="avatar h-left"> <img src="./core/images/default/default_avatar_thumb.png" alt="Monalinm" title="Monalinm" /> </a> <div class="lastpost-info"> <div class="lastpost-title-wrapper ellipsis"> <a 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polymerase using zero-mode waveguide (ZMW) optical confinement nanostructures or sequencing by binding (SBB)</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=42" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 385 Posts: 2,176 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/pacific-biosciences/325487-having-poor-coverage-depth-using-pacbio-hifi-ccs-reads-for-hdv-viral-genome#post325487" class="lastpost-title">Having poor coverage depth using Pacbio hifi ccs reads for HDV viral Genome.</a> </div> </td> <td class="topics-count">385</td> <td class="posts-count">2,176</td> <td class="lastpost"> <div class="lastpost-wrapper"> <a href="https://www.seqanswers.com/member/148795-osingh" class="avatar h-left"> <img src="./core/images/default/default_avatar_thumb.png" alt="Osingh" title="Osingh" /> </a> <div class="lastpost-info"> <div class="lastpost-title-wrapper ellipsis"> <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/pacific-biosciences/325487-having-poor-coverage-depth-using-pacbio-hifi-ccs-reads-for-hdv-viral-genome#post325487" class="lastpost-title">Having poor coverage depth using Pacbio hifi ccs reads for HDV viral Genome.</a> </div> <div class="lastpost-by"> by <a href="https://www.seqanswers.com/member/148795-osingh" data-vbnamecard="148795">Osingh</a> </div> <div class="lastpost-date post-date">02-20-2024, 05:21 AM</div> </div> </div> </td> </tr>  <tr class="forum-item main new" id="forum323995" data-channel-id="323995" data-lastcontent="0" data-readtime="0"> <td class="cell-forum"> <div class="forum-wrapper"> <div class="forum-info forum-info-has-rss"> <span class="icon b-icon b-icon__speech-double-large--blue h-left" title="Contains unread posts." ></span> <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/singular-genomics" class="forum-title">Singular Genomics</a> <div class="forum-desc">Optical ensemble sequencing by synthesis via fluorescently labeled reversible terminated nucleotides.</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=323995" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 0 Posts: 0 </div> </td> <td class="topics-count">0</td> <td class="posts-count">0</td> <td class="lastpost"> <div class="lastpost-wrapper"> </div> </td> </tr>  <tr class="forum-item main new" id="forum323996" data-channel-id="323996" data-lastcontent="0" data-readtime="0"> <td class="cell-forum"> <div class="forum-wrapper"> <div class="forum-info forum-info-has-rss"> <span class="icon b-icon b-icon__speech-double-large--blue h-left" title="Contains unread posts." ></span> <a href="https://www.seqanswers.com/forum/ultima-genomics" class="forum-title">Ultima Genomics</a> <div class="forum-desc">Open flow cell design on a circular wafer and mostly natural nucleotides that allow optical end-point detection without reversible terminators.</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=323996" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 0 Posts: 0 </div> </td> <td class="topics-count">0</td> <td class="posts-count">0</td> <td class="lastpost"> <div class="lastpost-wrapper"> </div> </td> </tr>  <tr class="forum-item main new" id="forum31" data-channel-id="31" data-lastcontent="1717064575" data-readtime="0"> <td class="cell-forum"> <div class="forum-wrapper"> <div class="forum-info forum-info-has-rss"> <span class="icon b-icon 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Post here</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=43" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 342 Posts: 1,701 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/applications-forums/de-novo-discovery/326130-hifiasm-and-sex-chromosomes-from-xy-specimen#post326130" class="lastpost-title">Hifiasm and sex chromosomes from XY specimen</a> </div> </td> <td class="topics-count">342</td> <td class="posts-count">1,701</td> <td class="lastpost"> <div class="lastpost-wrapper"> <a href="https://www.seqanswers.com/member/149716-andycarter" class="avatar h-left"> <img src="./core/images/default/default_avatar_thumb.png" alt="AndyCarter" title="AndyCarter" /> </a> <div class="lastpost-info"> <div class="lastpost-title-wrapper ellipsis"> <a href="https://www.seqanswers.com/forum/applications-forums/de-novo-discovery/326130-hifiasm-and-sex-chromosomes-from-xy-specimen#post326130" class="lastpost-title">Hifiasm and sex chromosomes from XY specimen</a> </div> <div class="lastpost-by"> by <a href="https://www.seqanswers.com/member/149716-andycarter" data-vbnamecard="149716">AndyCarter</a> </div> <div class="lastpost-date post-date">09-26-2024, 05:35 PM</div> </div> </div> </td> </tr>  <tr class="forum-item main new" id="forum45" data-channel-id="45" data-lastcontent="1712555455" data-readtime="0"> <td class="cell-forum"> <div class="forum-wrapper"> <div class="forum-info forum-info-has-rss"> <span class="icon b-icon b-icon__speech-double-large--blue h-left" title="Contains unread posts." ></span> <a href="https://www.seqanswers.com/forum/applications-forums/metagenomics" class="forum-title">Metagenomics</a> <div class="forum-desc">Ever wonder what's growing in that hot spring or glacier?</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=45" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 273 Posts: 852 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/applications-forums/metagenomics/325619-impact-of-biomass-on-shotgun-sequencing#post325619" class="lastpost-title">Impact of biomass on shotgun sequencing</a> </div> </td> <td class="topics-count">273</td> <td class="posts-count">852</td> <td class="lastpost"> <div class="lastpost-wrapper"> <a href="https://www.seqanswers.com/member/149482-donovan" class="avatar h-left"> <img src="./core/images/default/default_avatar_thumb.png" alt="Donovan" title="Donovan" /> </a> <div class="lastpost-info"> <div class="lastpost-title-wrapper ellipsis"> <a href="https://www.seqanswers.com/forum/applications-forums/metagenomics/325619-impact-of-biomass-on-shotgun-sequencing#post325619" class="lastpost-title">Impact of biomass on shotgun sequencing</a> </div> <div class="lastpost-by"> by <a href="https://www.seqanswers.com/member/149482-donovan" data-vbnamecard="149482">Donovan</a> </div> <div class="lastpost-date post-date">04-07-2024, 09:50 PM</div> </div> </div> </td> </tr>  <tr class="forum-item main new" id="forum39" data-channel-id="39" data-lastcontent="1702185430" data-readtime="0"> <td class="cell-forum"> <div class="forum-wrapper"> <div class="forum-info forum-info-has-rss"> <span class="icon b-icon b-icon__speech-double-large--blue h-left" title="Contains unread posts." ></span> <a href="https://www.seqanswers.com/forum/applications-forums/epigenetics" class="forum-title">Epigenetics</a> <div class="forum-desc">Any non-primary sequence heritable modification of genetic material. ChIP-SEQ, DNA methylation (Bisulfite-SEQ), chromatin modifications (methylation, acetylation, etc), non coding RNA.</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=39" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 357 Posts: 1,578 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/applications-forums/epigenetics/325286-tutorial-master-chip-seq-analysis-your-beginner-s-guide-to-bioinformatics#post325286" class="lastpost-title">Tutorial: Master ChIP seq analysis: Your beginner's guide to bioinformatics</a> </div> </td> <td class="topics-count">357</td> <td class="posts-count">1,578</td> <td class="lastpost"> <div class="lastpost-wrapper"> <a href="https://www.seqanswers.com/member/27261-crazyhottommy" class="avatar h-left"> <img src="./core/image.php?userid=27261&thumb=1&dateline=1687577514" alt="crazyhottommy" title="crazyhottommy" /> </a> <div class="lastpost-info"> <div class="lastpost-title-wrapper ellipsis"> <a href="https://www.seqanswers.com/forum/applications-forums/epigenetics/325286-tutorial-master-chip-seq-analysis-your-beginner-s-guide-to-bioinformatics#post325286" class="lastpost-title">Tutorial: Master ChIP seq analysis: Your beginner's guide to bioinformatics</a> </div> <div class="lastpost-by"> by <a href="https://www.seqanswers.com/member/27261-crazyhottommy" data-vbnamecard="27261">crazyhottommy</a> </div> <div class="lastpost-date post-date">12-09-2023, 09:17 PM</div> </div> </div> </td> </tr>  <tr class="forum-item main new" id="forum41" data-channel-id="41" data-lastcontent="1731652012" data-readtime="0"> <td class="cell-forum"> <div class="forum-wrapper"> <div class="forum-info forum-info-has-rss"> <span class="icon b-icon b-icon__speech-double-large--blue h-left" title="Contains unread posts." ></span> <a href="https://www.seqanswers.com/forum/applications-forums/rna-sequencing" class="forum-title">RNA Sequencing</a> <div class="forum-desc">Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=41" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 2,872 Posts: 11,430 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/applications-forums/rna-sequencing/326279-how-to-make-a-heatmap-with-the-exons-of-a-gene-with-bam-file-of-rna-seq-in-r-studio#post326279" class="lastpost-title">How to make a heatmap with the exons of a gene with bam file of RNA-seq in R-studio?</a> </div> </td> <td class="topics-count">2,872</td> <td class="posts-count">11,430</td> <td class="lastpost"> <div class="lastpost-wrapper"> <a href="https://www.seqanswers.com/member/150365-césar" class="avatar h-left"> <img src="./core/images/default/default_avatar_thumb.png" alt="César" title="César" /> </a> <div class="lastpost-info"> <div class="lastpost-title-wrapper ellipsis"> <a href="https://www.seqanswers.com/forum/applications-forums/rna-sequencing/326279-how-to-make-a-heatmap-with-the-exons-of-a-gene-with-bam-file-of-rna-seq-in-r-studio#post326279" class="lastpost-title">How to make a heatmap with the exons of a gene with bam file of RNA-seq in R-studio?</a> </div> <div class="lastpost-by"> by <a href="https://www.seqanswers.com/member/150365-césar" data-vbnamecard="150365">César</a> </div> <div class="lastpost-date post-date">11-14-2024, 10:26 PM</div> </div> </div> </td> </tr>  <tr class="forum-item main new" id="forum54" data-channel-id="54" data-lastcontent="1689932228" data-readtime="0"> <td class="cell-forum"> <div class="forum-wrapper"> <div class="forum-info forum-info-has-rss"> <span class="icon b-icon b-icon__speech-double-large--blue h-left" title="Contains unread posts." ></span> <a href="https://www.seqanswers.com/forum/applications-forums/clinical-sequencing" class="forum-title">Clinical Sequencing</a> <div class="forum-desc">Discuss issues unique to clinical sequencing.</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=54" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 42 Posts: 107 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/applications-forums/clinical-sequencing/324856-a-course-in-clinical-exome-analysis-any-recommendations#post324856" class="lastpost-title">A course in clinical exome analysis - 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Companies such as 23andme, Navigenics, Knome, DNADirect, and private efforts like the Personal Genome Project. Ethics, privacy, security, all of it goes here.</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=34" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 58 Posts: 291 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/personalized-genomics/personalized-genomics-aa/326268-hard-to-know-what-is-accurate-wes-and-wgs#post326268" class="lastpost-title">Hard to know what is accurate (WES and WGS)</a> </div> </td> <td class="topics-count">58</td> <td class="posts-count">291</td> <td class="lastpost"> <div class="lastpost-wrapper"> <a href="https://www.seqanswers.com/member/150351-oppletree" class="avatar h-left"> <img src="./core/images/default/default_avatar_thumb.png" alt="Oppletree" title="Oppletree" /> </a> <div class="lastpost-info"> <div class="lastpost-title-wrapper 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this forum.</div> </div> <a href="https://www.seqanswers.com/external?type=rss2&nodeid=27" title="View this forum's RSS feed." class="h-right h-margin-right-xl h-margin-top-xs h-hide-on-small"><span class="b-icon b-icon__rss"></span></a> </div> <div class="rx-forum-stats h-hide"> Topics: 48 Posts: 657 </div> <div class="rx-lastpost-info h-hide"> Last Post: <a href="https://www.seqanswers.com/forum/site-news/site-feedback-suggestions/325365-making-a-table-in-bbcode-does-not-work#post325365" class="lastpost-title">Making a table in BBCode does not work</a> </div> </td> <td class="topics-count">48</td> <td class="posts-count">657</td> <td class="lastpost"> <div class="lastpost-wrapper"> <a href="https://www.seqanswers.com/member/149114-hjarnek" class="avatar h-left"> <img src="./core/images/default/default_avatar_thumb.png" alt="hjarnek" title="hjarnek" /> </a> <div class="lastpost-info"> <div class="lastpost-title-wrapper ellipsis"> <a href="https://www.seqanswers.com/forum/site-news/site-feedback-suggestions/325365-making-a-table-in-bbcode-does-not-work#post325365" class="lastpost-title">Making a table in BBCode does not work</a> </div> <div class="lastpost-by"> by <a href="https://www.seqanswers.com/member/149114-hjarnek" data-vbnamecard="149114">hjarnek</a> </div> <div class="lastpost-date post-date">01-08-2024, 03:01 AM</div> </div> </div> </td> </tr> </table> <div class="list-footer"><a href="#" class="markchannelsread">Mark Channels Read</a></div> </div> </div> </div>  </div> </div>  <div class="canvas-widget-list section-1 js-sectiontype-secondary h-clearfix l-col__fixed-6 l-col__small--full has-adjacent-left-col h-padding-left-xl l-narrow-column">  <div class="b-module canvas-widget search-widget" id="widget_10" data-widget-id="14" data-widget-instance-id="10"> <div class="widget-header h-clearfix"> <div class="module-title h-left"> <h1 class="main-title js-main-title hide-on-editmode">Trending</h1> </div> <div class="module-buttons"> <span class="toggle-button module-button-item collapse" title="Collapse" data-toggle-title="Expand"><span class="b-icon b-icon__toggle-collapse">Collapse</span></span> </div> </div> <hr class="widget-header-divider" /> <div class="widget-content"> <ul class="conversation-list list-container h-clearfix"> <li class="b-post-sm js-post-sm" data-nodeid="326290"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/149680-novogene"> <img src="./core/image.php?userid=149680&thumb=1&dateline=1716924809" width="32" height="32" alt="Novogene" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/vendor-forum/326290-amplicon-vs-shotgun-metagenomics-–-how-do-i-choose">Amplicon vs Shotgun Metagenomics – How Do I Choose?</a> </div> <div class="b-post-sm__author"> by <a href="https://www.seqanswers.com/member/149680-novogene" data-vbnamecard="149680">Novogene</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> Metagenomics is the study of microbial communities in their original habitats and gives a comprehensive insight into the biochemical and metabolic interactions... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326290"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/vendor-forum">Sequencing Resources</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">11-21-2024, 02:02 PM</div> </div> </li> <li class="b-post-sm js-post-sm" data-nodeid="326273"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/149951-angelat"> <img src="./core/images/default/default_avatar_thumb.png" width="32" height="32" alt="AngelaT" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/forum/applications-forums/sample-prep-library-generation/326273-dna-turns-black-over-time-contamination">DNA turns black over time - contamination?</a> </div> <div class="b-post-sm__author"> by <a href="https://www.seqanswers.com/member/149951-angelat" data-vbnamecard="149951">AngelaT</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> Hi all! Thanks in advance for your help.<br /> <br /> In our lab we have been noticing something strange. Over time, some of our DNA samples turn a black... <div class="h-clearfix"> <div class="js-photo-preview photo-preview b-gallery-thumbnail-list b-gallery-thumbnail-list--preview"> <span class="b-gallery-thumbnail-list__item js-slideshow--item" itemscope itemtype="http://schema.org/ImageObject"> <img class="b-gallery-thumbnail-list__thumbnail" src="filedata/fetch?photoid=326274&type=thumb" title="" alt="" /> </span> </div> <div class="b-gallery-thumbnail-list__aside"> <span class="b-photo-count h-pointer js-slideshow--item"> <span class="b-photo-count__count">1</span> <span class="b-photo-count__label">Photo</span> </span> </div> </div> </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326273"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/forum/applications-forums/sample-prep-library-generation">Sample Prep / Library Generation</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">11-13-2024, 06:50 AM</div> </div> </li> <li class="b-post-sm js-post-sm" data-nodeid="326252"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/28554-ecseq-bioinformatics"> <img src="./core/images/default/default_avatar_thumb.png" width="32" height="32" alt="ecSeq Bioinformatics" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/forum/events-conferences/326252-online-workshop-a-practical-introduction-to-ngs-data-analysis-dec-9-11-2024">Online Workshop - A Practical Introduction to NGS Data Analysis (Dec 9-11, 2024)</a> </div> <div class="b-post-sm__author"> by <a href="https://www.seqanswers.com/member/28554-ecseq-bioinformatics" data-vbnamecard="28554">ecSeq Bioinformatics</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> <b><span style="font-size:20px">Online Course - A Practical Introduction to NGS Data Analysis</span></b><br /> <b><i>Quality Control, Read Mapping, Visualization and DNA Variant Analysis</i></b><br /> <br />... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326252"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/forum/events-conferences">Events / Conferences</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">11-04-2024, 07:31 AM</div> </div> </li> <li class="b-post-sm js-post-sm" data-nodeid="325521"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/149339-blainetoro"> <img src="./core/images/default/default_avatar_thumb.png" width="32" height="32" alt="blainetoro" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/oxford-nanopore/325521-actual-minimum-specs-for-an-oxford-nanopore-minion">Actual minimum specs for an Oxford Nanopore MinION?</a> </div> <div class="b-post-sm__author"> by <a href="https://www.seqanswers.com/member/149339-blainetoro" data-vbnamecard="149339">blainetoro</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> Hi all - I'm planning to purchase a MinION sequencer. It seems the IT requirements are fairly high (8GB RAM, i5/i7 processor, 500 GB SSD). I have a Macbook... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-325521"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/forum/sequencing-technologies-companies/oxford-nanopore">Oxford Nanopore</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">03-04-2024, 11:35 PM</div> </div> </li> </ul> <div class="widget-footer h-align-left h-clear"> <a href="https://www.seqanswers.com/search?r=78064134" class="b-button">View All</a> </div> </div> </div>   <div class="b-module js-module canvas-widget default-widget axd-widget axd-container" id="widget_193" data-widget-id="45" data-widget-instance-id="193"> <div class="widget-content restore axd axd_193"> <div class="axd-inner js-module__hide-if-empty "> <div class="ad_193_inner"> <span id=zone803333780></span> <script> (function(r,d,u) { var s = d.createElement(r); s.async = true; u += '&cb=' + Date.now() + Math.random(); s.src = u; var n = d.getElementsByTagName(r)[0]; n.parentNode.insertBefore(s, n); })('script',document,'//comparenetworks.engine.adglare.net/?803333780'); </script></div> </div> </div> </div>   <div class="b-module canvas-widget search-widget" id="widget_220" data-widget-id="12" data-widget-instance-id="220"> <div class="widget-header h-clearfix"> <div class="module-title h-left"> <h1 class="main-title js-main-title hide-on-editmode">Webinars</h1> </div> <div class="module-buttons"> <span class="toggle-button module-button-item collapse" title="Collapse" data-toggle-title="Expand"><span class="b-icon b-icon__toggle-collapse">Collapse</span></span> </div> </div> <hr class="widget-header-divider" /> <div class="widget-content"> <ul class="conversation-list list-container h-clearfix"> <li class="b-post-sm js-post-sm" data-nodeid="326254"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/146502-seqadmin"> <img src="./core/image.php?userid=146502&thumb=1&dateline=1673964993" width="32" height="32" alt="seqadmin" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/forum/site-news/webinar-series/326254-on-demand-webinars">On-Demand Webinars</a> </div> <div class="b-post-sm__author"> by <a href="https://www.seqanswers.com/member/146502-seqadmin" data-vbnamecard="146502">seqadmin</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> <span style="color:black">This fall was packed with four must-see webinars! If you missed out, they’re all available on demand for you to watch at your convenience.</span><br /> <br />... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326254"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/forum/site-news/webinar-series">Webinar Series</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">11-04-2024, 08:07 AM</div> </div> </li> <li class="b-post-sm js-post-sm" data-nodeid="326125"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/146502-seqadmin"> <img src="./core/image.php?userid=146502&thumb=1&dateline=1673964993" width="32" height="32" alt="seqadmin" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/forum/site-news/webinar-series/326125-single-cell-multi-omics-for-disease-research">Single-Cell Multi-Omics for Disease Research</a> </div> <div class="b-post-sm__author"> by <a href="https://www.seqanswers.com/member/146502-seqadmin" data-vbnamecard="146502">seqadmin</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> <br /> <br /> <br /> <br /> <br /> <b><a href="https://www.workcast.com/register?cpak=8003326921094844&referrer=SEQsite" target="_blank">Join us</a></b> for an exciting webinar focused on innovative research in the field of single-cell multi-omics. Our expert... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326125"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/forum/site-news/webinar-series">Webinar Series</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">09-26-2024, 06:22 AM</div> </div> </li> </ul> <div class="widget-footer h-align-left h-clear"> <a href="https://www.seqanswers.com/search?r=78064135" class="b-button">View All</a> </div> </div> </div>   <div class="b-module canvas-widget search-widget" id="widget_197" data-widget-id="12" data-widget-instance-id="197"> <div class="widget-header h-clearfix"> <div class="module-title h-left"> <h1 class="main-title js-main-title hide-on-editmode">Recent Articles</h1> </div> <div class="module-buttons"> <span class="toggle-button module-button-item collapse" title="Collapse" data-toggle-title="Expand"><span class="b-icon b-icon__toggle-collapse">Collapse</span></span> </div> </div> <hr class="widget-header-divider" /> <div class="widget-content"> <ul class="conversation-list list-container h-clearfix"> <li class="b-post-sm js-post-sm" data-nodeid="326260"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/146502-seqadmin"> <img src="./core/image.php?userid=146502&thumb=1&dateline=1673964993" width="32" height="32" alt="seqadmin" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/articles/326260-genetic-variation-in-immunogenetics-and-antibody-diversity">Genetic Variation in Immunogenetics and Antibody Diversity</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> <br /> <br /> <br /> The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben Martínez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326260"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/articles">Articles</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">11-06-2024, 07:24 PM</div> </div> </li> <li class="b-post-sm js-post-sm" data-nodeid="326184"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/146502-seqadmin"> <img src="./core/image.php?userid=146502&thumb=1&dateline=1673964993" width="32" height="32" alt="seqadmin" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/articles/326184-choosing-between-ngs-and-qpcr">Choosing Between NGS and qPCR</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> <br /> <br /> <br /> Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326184"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/articles">Articles</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">10-18-2024, 07:11 AM</div> </div> </li> <li class="b-post-sm js-post-sm" data-nodeid="326160"> <div class="b-post-sm__avatar avatar h-left"> <a href="https://www.seqanswers.com/member/146502-seqadmin"> <img src="./core/image.php?userid=146502&thumb=1&dateline=1673964993" width="32" height="32" alt="seqadmin" /> </a> </div> <div class="b-post-sm__content b-post-sm__content--has-avatar"> <div class="b-post-sm__title ellipsis"> <a href="https://www.seqanswers.com/articles/326160-non-coding-rna-research-and-technologies">Non-Coding RNA Research and Technologies</a> </div> <div class="b-post-sm__post-content post-content ellipsis restore"> <br /> <br /> <br /> <br /> Non-coding RNAs (ncRNAs) do not code for proteins but play important roles in numerous cellular processes including gene silencing, developmental pathways, and more. There are numerous types including microRNA (miRNA), long ncRNA (lncRNA), circular RNA (circRNA), and more. In this article, we discuss innovative ncRNA research and explore recent technological advancements that improve the study of ncRNAs.<br /> <br /> <span style="font-size:16px"><b>Nobel Prize for MicroRNA Discovery</b></span><br /> This week,... </div> <div class="list-item-footer h-clearfix"> <ul class="replylike_count h-left"> <li id="channel-326160"> <div class="channel h-left"> <div class="channel_text"> Channel: <a href="https://www.seqanswers.com/articles">Articles</a> </div> </div> </li> </ul> </div> <div class="b-post-sm__date">10-07-2024, 08:07 AM</div> </div> </li> </ul> <div class="widget-footer h-align-left h-clear"> <a href="https://www.seqanswers.com/search?r=78064136" class="b-button">View All</a> </div> </div> </div>   <div class="b-module canvas-widget search-widget" id="widget_204" data-widget-id="12" data-widget-instance-id="204"> <div class="widget-header h-clearfix"> <div class="module-title h-left"> <h1 class="main-title js-main-title hide-on-editmode">News</h1> 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