Monoclonal Antibody Epitope Binning Services

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We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects. </p> <b class="Highlights_tit">Highlights</b> <ul class="HighlightsArea"> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-1.jpg" srcset="static/images/Abouts-menu-lights-pic-1.webp 1x" width="200" height="131" loading="lazy"><a href="aboutus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>About CBL </a> </li> <li class="col-md-6 col-lg-6 col-sm-6"> <img src="static/images/Abouts-menu-lights-pic-2.jpg" srcset="static/images/Abouts-menu-lights-pic-2.webp 1x" width="200" height="131" loading="lazy"><a href="contactus.html" class="bannerbutton"> <i class="fa fa-angle-right"></i>Contact Us </a> </li> </ul> </li> </ul> </div> </div> </li> </ul> <script> window.addEventListener('load',function(){ var arr = ['/drug-discovery/therapeutics/','/bioconjugation/','/adc/']; $('.mennav ul li a').each(function(){ var $this = $(this); var href = $this.attr("href").toLocaleLowerCase(); $.each(arr, function(index, value) { if (href && href.startsWith(value)) { $this.attr('target', '_blank'); } }); }); }); </script> </div> <div class="top-search search-group"> <div class="col-md-5 col-sm-6"> <label class="radio-inline"><input type="radio" name="search" class="rdo_productsearch" value="product" data-type="product" placeholder="Please input cat#, product name, abbr, or keywords."> Product</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_servicesearch" value="service" data-type="service" placeholder="Please input your keywords."> Service</label> <label class="radio-inline"><input type="radio" name="search" class="rdo_googlesearch" value="google" data-type="google" placeholder="Please input your keywords." checked="checked"> Keyword</label> </div> <div class="col-md-7 col-sm-6 search-div"> <input id="keys" name="keys" placeholder="Please input your keywords." class="search-input"> <div class="selectItems"></div> <span class="search_btn"><i class="fa fa-search"></i></span> </div> </div> </div> <div class="searchShow"> <div class="searchtopbg"></div> <span class="searchclosed"><i class="fa fa-times"></i>Close</span> <div class="addsearch search-group"></div> </div> <div class="banner_inpage services_bg_2"> <div class="auto-container small_content-width about_container"> <ul class="bread-crumb clearfix"> <li><a href="/">Home</a></li> <li><a href="javascrpt:void(0)">Characterization</a></li><li><a href="javascrpt:void(0)">Binding Analysis</a></li> <li>Epitope Binning</li> </ul> <p>"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."</p> </div> </div> <script> window.addEventListener('load', function () { if (($('.bread-crumb li:last-child').text()) == "Peer-Reviewed Publications") { $(".banner_inpage").addClass("peer-reviewed-publications_bg").removeClass("services_bg_2"); } if ($(window).hasClass('bread-crumb')) { if ($(".bread-crumb").html().indexOf("RESOURCE") > -1) { $(".pronote").hide(); } if ($(".bread-crumb").html().indexOf("Careers") > -1) { $(".pronote").hide(); } } }); </script> <div class="small_content-width services-container"> <div class="col-md-9"> <div class="inpage_section_title"> <h1>Monoclonal Antibody Epitope Binning Services</h1> </div> <div class="page-tab"> <span onclick="window.location.href=window.location.pathname+'#tab1'">Background</span> <span onclick="window.location.href=window.location.pathname+'#tab2'">Platform</span> <span onclick="window.location.href=window.location.pathname+'#tab3'">Service</span> <span onclick="window.location.href=window.location.pathname+'#tab4'">Published Data</span> <span onclick="window.location.href=window.location.pathname+'#tab5'">FAQ</span> <span onclick="window.location.href=window.location.pathname+'#tab6'">Resources</span> </div> <script>window.addEventListener('load',function(){page_tab_margin();page_tab_css();})</script> <p> Epitope binning array of mAbs is a practical application of blocking assays in a diagnostic or research setting. The quest for a mAb that targets a specific region (epitope) on an antigen is often more important than the identification of a tightly-binding mAb, because affinity can be matured via standard protein engineering protocols. <b>Creative Biolabs</b> has been a leader in developing reproducible, high-throughput analytical techniques to characterize monoclonal antibodies by epitope binning. </p> <h2 class="h2_tit page_tab_margin" id="tab1"> Background </h2> <p> Antibodies are tested in a pairwise combinatorial manner, and those that compete for the same binding region are grouped together into bins. Antibodies that target similar epitopes often share similar functions. The ability to generate this information early in the drug discovery process enables researchers to “eliminate the funnel” by reducing the number of potential antibody candidates while maintaining epitope diversity. Binning assays can be highly informative to the discovery of therapeutic mAbs and can be predictive of functional activity. </p> <h2 class="h2_tit page_tab_margin" id="tab2"> Epitope Binning Platforms </h2> <p> Technical progress in human antibody discovery allows for the selection of hundreds of high affinity antibodies against many therapeutically relevant targets. <b>Creative biolabs</b> has introduced several emerging commercial platforms for epitope binning, including <b>ForteBio Octet platform, ProteOn array system, Biacore system </b>and <b>IBIS MX96 & CFM</b>, to handle binning of large numbers of antibodies simultaneously in an automated mode by redesigning the concept of a flow channel and the way in which samples are applied and/or delivered. </p> <h2 class="h2_tit page_tab_margin" id="tab3"> Epitope Binning Services </h2> <p class="show-right"> <img loading="lazy" width="250" height="337" alt="" src="static/img/Monoclonal-Antibody-Epitope-Binning-1.jpg" /> </p> <p> <b>Creative Biolabs</b> has the unprecedented ability to provide epitope binning services with over 99% accuracy and increasingly high efficiency. </p> <p> <strong>Services</strong> </p> <p> <b>Creative Biolabs</b> provides Monoclonal Antibody Epitope Binning services including but not limited to: </p> <ul class="ullist"> <li> <a href="Epitope-binning-by-ProteOn-Platform.html">Epitope binning by ProteOn platform</a> </li> <li> <a href="Epitope-binning-by-Biacore-Systems.html">Epitope binning by Biacore systems</a> </li> <li> <a href="Epitope-binning-by-ForteBio-Octet-platform.html">Epitope binning by Octet systems</a> </li> <li> <a href="/Epitope-Binning-by-IBIS-MX96-CFM.html">Epitope binning by IBIS MX96 & CFM</a> </li> </ul> <br /> <p> <strong>Key Benefits</strong> </p> <ul class="ullist"> <li> Label-Free, Real-Time Results </li> <li> High Throughput, Rapid Analysis </li> <li> High Quality Data at an Affordable Price </li> <li> Crude Sample Compatibility </li> <li> Less Sample Consumption </li> <li> Dedicated Data Acquisition and Analysis Software </li> </ul> <br /> <p> Alternatively, you can also choose an ELISA test as a basis for epitope binning. We provide sandwich ELISA and competitive ELISA. For cell-based assay, FACS analysis is frequently used for binning measurement. Whatever you need, <b>Creative Biolabs</b> can help! </p> <p> Other optional <a href="/Antibody-Analysis-Services.html">antibody analysis services</a>: </p> <ul class="ullist"> <li> <a href="/antibody-characterization.html">Antibody Characterization</a> </li> <li> <a href="/Antibody-Affinity-Measurement.html">Antibody Affinity Measurement</a> </li> <li> <a href="/antibody-sequencing-service.html">Hybridoma Sequencing Service</a> </li> <li> <a href="/next-generation-antibody-sequencing.html"><em>De Novo</em> Antibody Sequencing Services</a> </li> <li> <a href="/precision-paratope-mapping-service.htm">Precision Paratope Mapping Service</a> </li> <li> <a href="/SPR-Services.html">SPR Services</a> </li> <li> <a href="/Isothermal-Titration-Calorimetry-ITC-Service.html">Isothermal Titration Calorimetry (ITC) Services</a> </li> <li> <a href="/Differential-scanning-calorimetry-DSC-Service.html">Differential Scanning Calorimetry (DSC) Services</a> </li> <li> <a href="/Dual-Polarization-Interferometry-DPI-Services.html">Dual-Polarization Interferometry (DPI) Services</a> </li> </ul> <h2 class="h2_tit page_tab_margin" id="tab4"> Published Data </h2> <ul class="ullist"> <li> <strong>Human Antibody Epitope Map Containing Epitope Binning Data and Multiple Human mAb Structures</strong> </li> </ul> <p class="show-center"> <img loading="lazy" src="/static/img/1-2-3-6-monoclonal-antibody-epitope-binning-services-2.jpg" width="600" height="156" alt="" />Fig. 2 Functional activity and epitope bins of Pfs25 mAbs. (Niharika Shukla, 2023) </p> <p> Pfs25 is the main antigen of the malaria vaccine, which shows transmission blocking activity and induces a rapid decrease in antibody titer. A comprehensive analysis of all the transmission reduction epitopes of Pfs25 will provide information for vaccine structure-guided design and then the development of enhanced Pfs25-based vaccines. Here, the researchers compiled a detailed human antibody epitope map containing epitope binning data and the structure of a variety of human monoclonal antibodies. These structures demonstrate other epitopes in Pfs25 that can reduce transmission and extend this characterization to humans exposed to malaria. With this structural information, researchers will be able to develop more effective malaria vaccines. </p> <div class="reference"> <strong>Reference</strong> <ol class="ollist decimal"> <li> Shukla, Niharika, et al. "A human antibody epitope map of the malaria vaccine antigen Pfs25." <em>npj Vaccines</em> 8.1 (2023): 108. </li> </ol> </div> <script type="text/javascript"> window.addEventListener('load',function(){ faqlist(4); }); </script> <h2 class="h2_tit page_tab_margin" id="tab5"> FAQ </h2> <ol class="ollist faqlist"> <li> <span class="sFAQTit">What is epitope binning, and why is it important in monoclonal antibody characterization?</span> <p> Epitope binning is a technique used to categorize monoclonal antibodies based on their binding to specific epitopes on an antigen. This method helps in understanding the diversity of antibody responses and can inform the selection of antibodies with unique or overlapping binding sites. Epitope binning is crucial for identifying competitive interactions among antibodies, which is important for therapeutic antibody development and mechanism of action studies. </p> </li> <li> <span class="sFAQTit">How does epitope binning differ from other antibody characterization methods?</span> <p> Unlike general binding assays that only confirm an antibody's ability to bind to an antigen, epitope binning specifically evaluates how different antibodies relate to each other based on the epitope they recognize. It provides a detailed map of antibody-antigen interactions and can reveal whether antibodies are competitive, non-competitive, or bind to completely different sites. This level of detail is unique to epitope binning and is not typically achieved with other characterization techniques. </p> </li> <li> <span class="sFAQTit">What techniques are commonly used for epitope binning of monoclonal antibodies?</span> <p> Common techniques for epitope binning include Surface Plasmon Resonance (SPR), Bio-Layer Interferometry (BLI), and Enzyme-linked Immunosorbent Assay (ELISA) based approaches. SPR and BLI are particularly useful for real-time, label-free analysis of antibody interactions, providing insights into the kinetics and competition of antibody binding. </p> </li> <li> <span class="sFAQTit">Can epitope binning be used for all types of antigens?</span> <p> Epitope binning can be applied to a wide range of antigens, including proteins, peptides, and complex structures like viruses or cells. However, the complexity of the antigen can affect the choice of technique and the interpretation of the data. For complex antigens, it may be necessary to use more than one method or additional analytical tools to fully understand the antibody-antigen interactions. </p> </li> <li> <span class="sFAQTit">What role does epitope binning play in therapeutic antibody development?</span> <p> Epitope binning helps in selecting antibodies that target unique and therapeutically relevant epitopes. By identifying non-overlapping epitopes, researchers can choose antibodies that might offer synergistic effects when used in combination therapies. Additionally, understanding the epitope landscape can aid in avoiding cross-reactivity with related antigens, potentially reducing side effects and improving drug specificity. </p> </li> <li> <span class="sFAQTit">How can epitope binning contribute to improving antibody affinity and specificity?</span> <p> Through epitope binning, researchers can identify antibodies that bind to different regions of an antigen. By understanding these binding patterns, it is possible to engineer antibodies with improved affinity and specificity by modifying them to enhance binding characteristics or by selecting those that target more accessible or therapeutically relevant epitopes. This targeted selection and modification can lead to the development of highly effective and specific antibody therapies. </p> </li> <li> <span class="sFAQTit">What challenges are associated with epitope binning in monoclonal antibody characterization?</span> <p> One of the main challenges in epitope binning is the complexity of antigen structure. Some antigens may have multiple epitopes closely located, making it difficult to distinguish between the binding sites of different antibodies. Additionally, technical limitations in assay conditions, such as antigen presentation and assay sensitivity, can affect the accuracy and resolution of epitope binning results. Overcoming these challenges often requires optimizing experimental conditions and possibly combining multiple characterization techniques. </p> </li> <li> <span class="sFAQTit">How does epitope binning aid in patenting and intellectual property (IP) creation for monoclonal antibodies?</span> <p> Epitope binning can provide critical data that strengthens patent claims by clearly demonstrating the unique binding characteristics of a monoclonal antibody. By showing that an antibody binds to a unique epitope not targeted by existing antibodies, developers can establish the novelty and non-obviousness of their antibody products, which are key criteria for patentability. This detailed characterization can be invaluable in securing IP rights and can help in protecting the commercial interests of new therapeutic antibodies. </p> </li> </ol> <h2 class="h2_tit page_tab_margin" id="tab6"> Resources </h2> <p> Use the resources in our library to help you understand your options and make critical decisions for your study. </p> <div class="tab_nav"> <span class="tab_choosebg">Videos</span><span>Podcasts</span><span>Infographic</span><span>Flyer</span><span>Case study</span><span>Articles</span> </div>  <div class="tab_cont" style="display:block;"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/introduction-of-recombinant-antibody.jpg" alt="" /> </div> <a href="https://youtu.be/0-yZ9dTGg18" target="_blank">Introduction of Recombinant Antibody</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/immunogenicity-overview-of-therapeutic-biologics.jpg" alt="" /> </div> <a href="https://youtu.be/J430752_dTo" target="_blank">Immunogenicity overview of therapeutic biologics</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/video-development-of-monoclonal-antibodies-s.png" alt="" /> </div> <a href="https://youtu.be/NGqipRkxXiY" target="_blank">Antibody Library Screening - Creative Biolabs</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/antibody-based-cancer-therapies-creative-biolabs.jpg" alt="" /> </div> <a href="https://youtu.be/_Cqj5gphNug" target="_blank">Antibody based Cancer Therapies - Creative Biolabs</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/video-de-novo-antibody-sequencing-service.jpg" alt="" /> </div> <a href="https://youtu.be/lcdGTNKbaGQ" target="_blank"><em>De Novo</em> Antibody Sequencing Service</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/video-100-identification-of-leucine-and-isoleucine-in-de-novo-antibody-sequencing.jpg" alt="" /> </div> <a href="https://youtu.be/lcdGTNKbaGQ" target="_blank">100% Identification of Leucine and Isoleucine in <em>De Novo</em> Antibody Sequencing</a> </div> </div> </div>  <div class="tab_cont"> <div class="therapy_ad"> <a href="therapeutic-antibody.html"> <img loading="lazy" src="../static/img/podcasts-ad.png" width="1700" height="500" srcset="../static/img/podcasts-ad.webp 1x,../static/img/podcasts-ad.webp 2x,../static/img/podcasts-ad.webp 3x" alt="In this brand new podcast series, we will show you everything you want to know about CAR-T therapy, including the mechanism, current applications, technology limitations, and potential strategies." /></a> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/A-Brief-Introduction-to-Antibodies-1.jpg" width="600" height="500" alt="" /> </div> <a href="https://go.creative-biolabs.com/a-brief-introduction-to-antibodies.html">A Brief Introduction to Antibodies</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Antibody-Library-Screening-Creative-Biolabs.jpg" width="600" height="500" alt="" /> </div> <a href="https://go.creative-biolabs.com/antibody-library-screening.html ">Antibody Library Screening</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/De-Novo-Antibody-Seuqencing-Service-Creative-Biolabs.jpg" width="600" height="500" alt="" /> </div> <a href="https://go.creative-biolabs.com/de-novo-antibody-sequencing-service.html"><em>De Novo</em> Antibody Sequencing Service</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Antibody-Based-Therapies-1.jpg" width="600" height="500" alt="" /> </div> <a href="https://go.creative-biolabs.com/antibody-based-therapies.html">Antibody-Based Therapies</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Magic-Therapeutic-Antibody-Discovery-Platform-Flyer-Creative-Biolabs.jpg" width="600" height="500" alt="" /> </div> <a href="/resource/pdf/com/flyers/Magic-Therapeutic-Antibody-Discovery-Platform-Flyer-Creative-Biolabs.pdf">Magic<sup>TM</sup> Therapeutic Antibody Discovery Platform</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit_2"> <img loading="lazy" src="/resource/images/Antibody-Development-Case-Study-com.jpg" width="530" height="500" alt="" /> </div> <a href="https://go.creative-biolabs.com/antibody-development-case-study.html">Antibody Development Case Study</a> </div> </div> </div>  <div class="tab_cont"> <div class="row pagerow_pt"> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/what-is-antibody-de-novo-sequencing-1.jpg" width="600" height="500" alt="" /> </div> <a href="/what-is-antibody-de-novo-sequencing.html">What Is Antibody <em>De Novo</em> Sequencing?</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/what-is-protein-mass-spectrometry-technology-1.jpg" width="600" height="500" alt="" /> </div> <a href="/what-is-protein-mass-spectrometry-technology.html">What Is Protein Mass Spectrometry Technology?</a> </div> <div class="col-md-4"> <div class="pagerow_pt_unit"> <img loading="lazy" src="../static/img/hybridoma-sequencing-technology-assisting-the-r-d-and-production-1.jpg" width="600" height="500" alt="" /> </div> <a href="/hybridoma-sequencing-technology-assisting-with-the-r-d-and-production-of-monoclonal-antibodies.html">Hybridoma Sequencing Technology - 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