Leo System (004-550) by ProteinSimple, Part of Bio-Techne

<!DOCTYPE html> <html lang="en" dir="ltr" prefix="og: https://ogp.me/ns#"> <head> <meta charset="utf-8" /> <noscript><style>form.antibot * :not(.antibot-message) { display: none !important; }</style> </noscript><meta name="applicable-device" content="pc,mobile" /> <link rel="canonical" href="https://www.bio-techne.com/p/simple-western/leo-system_004-550" /> <meta property="og:site_name" content="Bio-Techne" /> <meta property="og:type" content="Website" /> <meta property="og:url" content="https://www.bio-techne.com/" /> <meta name="Generator" content="Drupal 10 (https://www.drupal.org)" /> <meta name="MobileOptimized" content="width" /> <meta name="HandheldFriendly" content="true" /> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <script type="application/ld+json">{ "@context": "https://schema.org", "@graph": [ { "@type": "WebPage", "@id": "https://www.bio-techne.com/p/simple-western/leo-system_004-550", "breadcrumb": { "@type": "BreadcrumbList", "itemListElement": [ { "@type": "ListItem", "position": 1, "name": "Home", "item": "https://www.bio-techne.com/" }, { "@type": "ListItem", "position": 2, "name": "Simple Western", "item": "https://www.bio-techne.com/search?keywords=Simple%20Western\u0026category_attr=Simple%20Western" } ] } } ] }</script> <meta http-equiv="x-dns-prefetch-control" content="on" /> <meta http-equiv="x-ua-compatible" content="ie=edge" /> <meta name="keywords" content="" /> <script type="application/ld+json" id="json-ld">[{"@context":"http://schema.org","@type":"Product","additionalProperty":[],"aggregateRating":[],"alternateName":"","brand":{"@type":"Brand","name":"ProteinSimple"},"description":"The Leo\u2122 System, powered by Simple Western\u2122 Technology, is a high-throughput, fully automated capillary western platform. It enables fast, simple, and sensitive protein quantification and detection, processing up to 100 samples per run, increasing experimental size for quantitative assay designs and delivering results in as little as 3 hours.","image":[{"@type":"ImageObject","url":"https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-2272024113851.png","description":"The Leo\u2122 System, powered by Simple Western\u2122 Technology, is a high-throughput, fully automated capillary western platform. It enables fast, simple, and sensitive protein quantification and detection, processing up to 100 samples per run, increasing experimental size for quantitative assay designs and delivering results in as little as 3 hours. 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Everything happens inside a capillary, and the Leo precisely controls the protein separation and reagent additions and incubations, even the detection.\n\nRun larger experiments for analytical grade protein quantitation.\nWith space for up to 96 unique samples and 4 molecular weight ladders per run, now you can run full standard curves, quality control (QC) samples, blanks, controls, references, and replicates alongside your unknown samples for robust analytical-grade quantitation of protein biomarker concentration and protein expression."},{"@type":"ImageObject","url":"https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-2272024125313.png","description":"Leo separates sample proteins by molecular weight before immunodetection, offering the ability to resolve non-specific binding and impart additional specificity in peak detection compared to other quantitative immunoassays that rely solely on immunodetection. After separation and immobilization of sample proteins, the sample matrix is flushed thoroughly from the capillary, so immunodetection occurs within an idealized buffer environment, minimizing cross-reactivity and non-specific binding for complex sample analysis."},{"@type":"ImageObject","url":"https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-2272024125359.png","description":"Simple Western is the only quantitative immunoassay technology that adds electrophoretic separation of sample proteins before immunodetection, enabling quantification of protein isoforms that differ in size. Using Simple Western technology, isoforms can be separated by size and subsequently detected using pan-target antibodies that bind to all isoforms present in the sample. The Leo System provides robust quantitative immunoassays that distinguish intact vs cleaved isoforms, fragments, aggregates, degradation products, oligomers, and more."},{"@type":"ImageObject","url":"https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-227202413353.png","description":"Detect up to 8 targets from just 3\u20135 \u00b5L of sample. Use the RePlex\u2122 Module, an automated stripping and re-probing replacement, to run two immunoassays in series or run an immunoassay followed by total protein detection for more accurate protein expression normalization. Plus, resample up to 4 times from the same well for analysis in independent capillaries, thus avoiding any concerns about antibody cross reactivity."},{"@type":"ImageObject","url":"https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-237202417655.png","description":"Determine optimal assay conditions on the Leo System in as little as a day. You can test multiple sample concentrations, antibody titration series, and positive and negative controls in 100 independent capillaries in one run. In addition, Leo includes high dynamic range (HDR) detection, like Jess and Abby."},{"@type":"ImageObject","url":"https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-2372024171048.png","description":"Maintain flexibility in your assay design and throughput as you progress from discovery through IND and into commercialization. You have the option to run 1-4 cartridges (25-100 samples) per run and detect up to 8 targets per sample. Start developing your assay on the Leo System with one cartridge at a time and scale up to maximal throughput when needed. Leo allows you to design assays capable of relative or absolute protein quantitation. Save time by developing and scaling up on the same system, enabling you to reach your next milestone faster.\nConfidence to meet regulatory guidelines for bioanalytical method validation.\nCompass\u2122 Software for Simple Western\u2122 Technology is the software package that all Simple Western instruments use to acquire, manage, and analyze data. Compass for Simple Western Software 7.0 has all the tools required for 21 CFR Part 11 compliance. Leo can boost your confidence to generate data that meets regulatory guidelines for bioanalytical method validation."}],"isRelatedTo":[],"itemcondition":"new","mpn":"004-550","name":"Leo System","productID":"004-550","review":[],"sku":"004-550","url":"https://www.bio-techne.com/p/simple-western/leo-system_004-550"},{"@context":"http://schema.org","@type":"AskAction","question":[]}]</script> <meta name="description" content="The Leo system, powered by Simple Western Technology, is a high-throughput, fully automated capillary western. 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<div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" title="Our capillary electrophoresis instruments (icIEF and CE-SDS) provide reproducible quantitative analysis for thorough therapeutic protein characterization." class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single-Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> <a href="/applications/crispr" class="dropdown-item text-wrap" 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data-drupal-link-system-path="node/1452">Organoid and 3-D Culture</a> <a href="/research-areas/stem-cells" class="dropdown-item text-wrap" data-drupal-link-system-path="node/161">Stem Cells</a> <a href="/research-areas/targeted-protein-degradation" class="dropdown-item text-wrap" data-drupal-link-system-path="node/376">Targeted Protein Degradation</a> <a href="/research-areas" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6741">View All Research Areas</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-therapeutic-manufacturing" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Therapeutic Manufacturing <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-therapeutic-manufacturing" aria-labelledby="navbarDropdown-therapeutic-manufacturing"> <div class="row"> <div class="col 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Proteins</a> <a href="/gmp-products/gmp-small-molecules" class="dropdown-item text-wrap" data-drupal-link-system-path="node/366">GMP Small Molecules</a> <a href="/gmp-products/gmp-antibodies" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13266">GMP Antibodies</a> <a href="/gmp-products/gmp-capabilities" class="dropdown-item text-wrap" title="GMP Capabilities" data-drupal-link-system-path="node/1071">GMP Capabilities</a> <a href="/gmp-products/ruo-to-gmp" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3421">Transition to GMP </a> <a href="/gmp-products/loa-request" class="dropdown-item text-wrap" data-drupal-link-system-path="node/13326">DMF/LOA Request</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instruments<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/brands/proteinsimple" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3281">ProteinSimple Instruments</a> <a href="/instruments/ice" class="dropdown-item text-wrap" data-drupal-link-system-path="node/256">iCE Maurice</a> <a href="/instruments/imagers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/536">Imagers</a> <a href="/instruments/luminex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/241">Luminex</a> <a href="/instruments/micro-flow-imaging" class="dropdown-item text-wrap" data-drupal-link-system-path="node/526">Micro-Flow Imaging</a> <a href="/instruments/simple-plex" class="dropdown-item text-wrap" data-drupal-link-system-path="node/541">Simple Plex Ella</a> <a href="/instruments/simple-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/521">Simple Western</a> <a href="/instruments/single-cell-dispensers" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6361">Single Cell Dispensers</a> <a href="/instruments/single-cell-western" class="dropdown-item text-wrap" data-drupal-link-system-path="node/531">Single Cell Western</a> <a href="/instruments" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6731">View All Instruments</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Applications<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/methods/bioprocessing" class="dropdown-item text-wrap">Bioprocessing</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Research Areas<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/research-areas/cell-and-gene-therapy" class="dropdown-item text-wrap" data-drupal-link-system-path="node/51">Cell & Gene Therapy</a> <a href="/applications/immuno-oncology-clinical" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2066">Immuno-Oncology</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-diagnostics" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Diagnostics <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-diagnostics" aria-labelledby="navbarDropdown-diagnostics"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Controls & Reagents<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/clinical-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7606">Clinical Quality Controls</a> <a href="/diagnostics/molecular-quality-controls" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5596">Molecular Controls</a> <a href="/diagnostics/controls-reagents/rnascope-ish-probe-high-risk-hpv" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5981">RNAscope™ ISH Probe High Risk HPV</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Kits<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/amplidex-genetic-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5581">Genetics</a> <a href="/diagnostics/quantidex-oncology-testing-kits" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5586">Oncology</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Technology<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/exosome" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5486">Exosome Platform</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> OEM Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/ivd-assay-development" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10881">IVD Assay Development</a> <a href="/diagnostics/ivd-contract-manufacturing" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7381">IVD Contract Manufacturing</a> <a href="/diagnostics/molecular-quality-controls/oem-and-commercial-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6781">Molecular Diagnostic OEM Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Companion Diagnostics<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/diagnostics/companion-diagnostics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5631">Companion Diagnostics Services</a> <a href="/diagnostics/companion-diagnostics/multiomics" class="dropdown-item text-wrap" data-drupal-link-system-path="node/10616">Multiomics Platform</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-services" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Services <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-services" aria-labelledby="navbarDropdown-services"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Quotes<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/bulk-quotes" class="dropdown-item text-wrap" data-drupal-link-system-path="services/bulk-quotes">Bulk Quote Discounts</a> <a href="/services/custom-formulation" class="dropdown-item text-wrap" data-drupal-link-system-path="services/custom-formulation">Custom Formulation Quotes</a> <a href="/reagents/rnascope-ish-technology/probes/custom" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12571">RNAscope Custom Probe Design Quote</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Custom Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/custom-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1891">All Custom Services</a> <a href="/services/custom-antibody-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/906">Custom Antibody Services</a> <a href="/services/custom-cell-and-gene-therapy-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1281">Custom Cell & Gene Therapy Services</a> <a href="/services/custom-compound-library-service" class="dropdown-item text-wrap" data-drupal-link-system-path="node/2126">Custom Compound Library Services</a> <a href="/services/custom-elisa-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/261">Custom ELISA Services</a> <a href="/services/gene-engineering-services-tcbuster" class="dropdown-item text-wrap" title="Gene Engineering Services: Bio-Techne" data-drupal-link-system-path="node/1136">Custom Gene Engineering Services</a> <a href="/services/custom-luminex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/271">Custom Luminex Services</a> <a href="/services/custom-protein-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1441">Custom Protein Services</a> <a href="/services/custom-simple-plex-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/6296">Custom Simple Plex Services</a> <a href="/services/custom-targeted-protein-degradation-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/861">Custom Targeted Protein Degradation Services</a> <a href="/services/professional-assay-services-rnascope" class="dropdown-item text-wrap" data-drupal-link-system-path="node/12306">RNAscope Professional Assay Services</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Instrument Services<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/certified-service-provider-program" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5446">Certified Service Provider (CSP) Program</a> <a href="/resources/instrument-services" class="dropdown-item text-wrap" data-drupal-link-system-path="node/5471">Contract Services</a> <a href="/services/instrument-services/simple-western-service-plans" class="dropdown-item text-wrap" data-drupal-link-system-path="node/3181">Simple Western Service Plans</a> </div> </div> </div> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Licensing<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/services/biotechnology-licensing-partnering" class="dropdown-item text-wrap" data-drupal-link-system-path="node/1101">Biotechnology Licensing and Partnering</a> </div> </div> </div> </div> </div> </li> <li class="nav-item dropdown"> <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown-resources" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false"> Resources <span class="icon-bt-icon-caret-cropped menu-caret js-caret d-inline-block"></span> </a> <div class="dropdown-menu dropdown-menu-resources" aria-labelledby="navbarDropdown-resources"> <div class="row"> <div class="col sub-menu-wrapper"> <div class="sub-menu-header"> Product & Instrument Tools<span class="icon-keyboard_arrow_down submenu-caret"></span> <hr/> <div class="menu-children-wrap sub-menu-box"> <a href="/resources/cofa-finder-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/cofa-finder-tool">Certificate of Analysis (COA) Finder Tool</a> <a href="/resources/calculators" class="dropdown-item text-wrap" data-drupal-link-system-path="node/7416">Calculators</a> <a href="/resources/flow-cytometry-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="node/8821">Flow Cytometry Panel Builder</a> <a href="/luminex-assay-customization-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="luminex-assay-customization-tool">Luminex Assay Customization Tool</a> <a href="/resources/product-insert-lookup-tool" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/product-insert-lookup-tool">Product Insert Lookup Tool</a> <a href="/resources/rnascope-citations" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/rnascope-citations">RNAscope Citations</a> <a href="/resources/simple-plex-panel-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-plex-panel-builder">Simple Plex Panel Builder</a> <a href="/resources/simple-western-kit-builder" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-western-kit-builder">Simple Western Kit Builder</a> <a href="/resources/simple-western-antibody-database" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/simple-western-antibody-database">Simple Western Antibody Database</a> <a href="/resources/single-cell-western-antibody-database" class="dropdown-item text-wrap" data-drupal-link-system-path="resources/single-cell-western-antibody-database">Single-Cell Western Antibody Database</a> <a href="/resources/spectra-viewer" class="dropdown-item text-wrap" 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<div class="highlighted"> <aside class="container section clearfix" role="complementary"> <div> </div> </aside> </div> <div id="main" class="container-fluid container-wide "> <div class="row"> <div class="col-md-12"> <div> <div id="block-bio-techne-global-breadcrumbs" class="system_breadcrumb_block"> <nav role="navigation" aria-labelledby="system-breadcrumb"> <h2 id="system-breadcrumb" class="visually-hidden">Breadcrumb</h2> <ol> <li> <a href="/">Home</a> </li> <li> Products </li> <li> <a href="/search?keywords=Simple%20Western&category_attr=Simple%20Western">Simple Western</a> </li> <li> Leo System (004-550) </li> </ol> </nav> </div> </div> </div> </div> <div class="row"> <div class="col-md-12"> <main> <section class="section"> <div> <div id="block-bio-techne-global-content" class="system_main_block"> <div class="top_region"><div class="o___product-summary mb-3 border-bottom"><div id="pp-page-title-brand-wrapper"> <div id="header-subheader" data-section="section-base--header-subheader"> <div class="row" id="pp-page-title-brand-wrapper"> <div class="col-lg-9 col-md-12"> <h1 class="font-weight-bold"> Leo System </h1> <h2 class="font-weight-normal h4"> ProteinSimple, part of Bio-Techne <span id="product-code"> | Catalog # <span class="font-weight-bold">004-550</span> </span> </h2> <div id="pp-reviews-citations-row"> <div id="header_left" class="row"> <div class="col-auto d-flex align-self-center"> <div> </div> </div> </div> </div> <div id="new_version_available"> </div> </div> <div class="col-lg-3 col-md-12"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/logos/bio-techne-proteinsimple.svg" alt="ProteinSimple, part of Bio-Techne" class="d-flex h-100 w-100"> </div> </div> </div> </div> </div> <div class="row mb-4"><div class="col-xl-3 order-xl-first" id="image_wrapper"><div id="images_summary"><article class="product-data-wrapper"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand="proteinsimple"> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-2272024113851.png" alt="The Leo™ System, powered by Simple Western™ Technology" title="The Leo™ System, powered by Simple Western™ Technology" data-caption="The Leo™ System, powered by Simple Western™ Technology, is a high-throughput, fully automated capillary western platform. It enables fast, simple, and sensitive protein quantification and detection, processing up to 100 samples per run, increasing experimental size for quantitative assay designs and delivering results in as little as 3 hours. " data-badges="" data-application="Simple Western" width="250" height="250" fetchpriority=high as=image class="search_result_thumbnail_image" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </article> </div> </div> <div class="col-xl-9 order-xl-first" id="key_benefits_buttoon_wrapper"> <h3> Leo Provides High-throughput Quantitative Western Analysis – Shipping available in 2025! </h3> <article class="product-data-wrapper "> The Leo™ System, powered by Simple Western™ Technology, is a high-throughput, fully automated capillary western platform. It enables fast, simple, and sensitive protein quantification and detection, processing up to 100 samples per run, increasing experimental size for quantitative assay designs and delivering results in as little as 3 hours. </article> <div id="pp-orderblock-buttons"><div class="float-right mt-3"> <a class="btn a___button-primary m-1 mt-3 col-xs-4" href="/p/instruments/simple-western/request-quote?productName=Leo%20System&catalogNumber=004-550" target="_self"> Request Pricing </a> </div> </div> </div> <div class="col-xl-3" id="product_ads_wrapper"><article class="product-data-wrapper"> </article> </div> </div> </div> <div><div id="instrument_meet_section"><div id="instrument_meet_section" class="top_region"> <div class="row"> <div class="col-md-6"> <h3 class="mb-2">How Does Leo Work?</h3> Leo, built on proven and trusted Simple Western technology, processes up to 100 samples in a single run, with a processing time of 3 hours, greatly increasing experimental throughput and enabling larger experiments for analytical grade protein quantification. Get the quantitative capabilities of immunoassays with the size resolution and specificity of westerns. Leo offers flexibility with the ability to utilize 1-4 capillary cartridges to run 25-100 samples per run and detect up to 8 protein targets per sample. Each well can be resampled up to 4 times and analyzed in a separate capillary, thus avoiding any concerns with carryover of samples from a prior sampling. Invest confidently in one instrument that meets your needs through the full drug development process. </div> <div class="col-md-6" > <div class="embed-responsive embed-responsive-16by9"> <iframe width="560" height="315" src="https://www.youtube.com/embed/WIWFwvSxFRU" allow="accelerometer; encrypted-media; gyroscope; picture-in-picture" allowfullscreen> </iframe> </div> </div> </div> </div> </div> </div> <div><div id="instrument_additional_section"> <div class="paragraph paragraph--type--product-page-content paragraph--view-mode--full"> <div> <div> <div class="paragraph paragraph--type--card-paragraph paragraph--view-mode--default card-para-default"> <div class="row card_row"> <div class="col-md-6"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="https://embed.envoke-demos.com/bio-techne/leo_platform/web.html" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/instruments/leo-3d-tour.png" alt="Leo 3D Tour" loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://embed.envoke-demos.com/bio-techne/leo_platform/web.html" > <h3 class="card_title_htag"> <div><h3>Take a Virtual Tour of Leo</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Explore Leo for free with an immersive 3D experience and navigate Leo's features at your own pace in a virtual environment. </p></div> </div> <div class="card-bottom-link"> <div> <a href="https://embed.envoke-demos.com/bio-techne/leo_platform/web.html" > Take 3D Tour </a> </div> </div> </div> </div> </div> <div class="col-md-6"> <div class="h-100"> <div class="d-flex flex-column h-100"> <a href="https://info.bio-techne.com/introducing-the-future-of-western-analysis.html" > <div class="card-img-top text-center mb-3" style="background-repeat: no-repeat; background-color: ; height:200px; width: 100%;"> <div class="teaser-valign-container" style=""> <div class="valign"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/videos/leo-launch-event.png" alt="Watch the replay of the Leo launch event to explore how Leo can meet your needs, from discovery through analytical development to commercialization, and enable you to reach your next milestone faster." loading="lazy" > </div> </div> </div> </a> <a class="card_title_link" href="https://info.bio-techne.com/introducing-the-future-of-western-analysis.html" > <h3 class="card_title_htag"> <div><h3>Watch the Leo Launch Event</h3></div> </h3> </a> <div class="card_description flex-grow-1"> <div><p>Watch the replay of the Leo launch event to explore how Leo can meet your needs and reach your next milestone faster, from discovery through analytical development to commercialization.</p></div> </div> <div class="card-bottom-link"> <div> <a href="https://info.bio-techne.com/introducing-the-future-of-western-analysis.html" > Watch Now </a> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> </div> <div class="tab-content"><div id="about-data-tab-tab"></div> <ul class="nav nav-tabs nav-fill " id="productTab" role="tablist"> <li class="nav-item"> <a class="nav-link active" id="about-leo-system-tab-tab" href="#about-leo-system-tab" data-toggle="tab" role="tab" aria-controls="region-4171591922717" aria-selected="true" >About Leo System</a> </li> <li class="nav-item"> <a class="nav-link" id="citations-tab-tab" href="#citations-tab" data-toggle="tab" role="tab" aria-controls="region-8961620056042" aria-selected="false" >Citations / Testimonials (32)</a> </li> </ul> <div class="tab-content rounded-bottom border-left border-right border-bottom mb-3" id="ProductTabContent"><div class="tab-pane fade show active mx-4" id="about-leo-system-tab" data-tab-label="About Leo System" role="tabpanel" aria-labelledby="about-leo-system-tab-tab"><div class="product_details_anchors"><div class="product-pages-anchors nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"><li id="how-can-leo-system-help-you_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor active" href="#how-can-leo-system-help-you" id="how-can-leo-system-help-you_li" >How Can Leo System Help You?</a> </li> <li id="how-does-leo-system-work_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#how-does-leo-system-work" id="how-does-leo-system-work_li" >How Does Leo System Work?</a> </li> <li id="applications_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#applications" id="applications_li" >Applications</a> </li> <li id="products-compatible-with-leo-system_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#products-compatible-with-leo-system" id="products-compatible-with-leo-system_li" >Products Compatible with Leo System</a> </li> <li id="specifications_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 jump-anchor" href="#specifications" id="specifications_li" >Specifications</a> </li> <li id="product-documents_li" class="nav-item m-0"> <a class="nav-link py-0 px-2 product-documents" href="#product-documents" id="product-documents_li" >Product Documents</a> </li> </div> <div id="how-can-leo-system-help-you" class="nav-link px-0" data-anchor-label="How Can Leo System Help You?"><table class="product-data-wrapper tablesaw tablesaw-stack table" id="data_image_table_wrapper"><h3>How Can Leo System Help You?</h3><article class="product-data-wrapper"> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-2272024121030.png" data-srcset="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-2272024121030.png" alt="Maximize throughput and process more samples in a day." title="Maximize throughput and process more samples in a day." data-caption="Leo can dramatically increase your throughput, with analysis of up to 100 samples per 3 hours run so you can get 4-times the throughput of Jess™ or Abby™ Systems and 5-times the throughput of Peggy Sue or Sally Sue Systems. Like other Simple Western Systems, Leo automates the protein separation and immunodetection of traditional western blotting, giving you fully analyzed results in just 3 hours. Everything happens inside a capillary, and the Leo precisely controls the protein separation and reagent additions and incubations, even the detection. <br /><br /> <strong>Run larger experiments for analytical grade protein quantitation.</strong><br /> With space for up to 96 unique samples and 4 molecular weight ladders per run, now you can run full standard curves, quality control (QC) samples, blanks, controls, references, and replicates alongside your unknown samples for robust analytical-grade quantitation of protein biomarker concentration and protein expression." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Maximize throughput and process more samples in a day.</h4> <p> Leo can dramatically increase your throughput, with analysis of up to 100 samples per 3 hours run so you can get 4-times the throughput of Jess™ or Abby™ Systems and 5-times the throughput of Peggy Sue or Sally Sue Systems. Like other Simple Western Systems, Leo automates the protein separation and immunodetection of traditional western blotting, giving you fully analyzed results in just 3 hours. Everything happens inside a capillary, and the Leo precisely controls the protein separation and reagent additions and incubations, even the detection. <br><br> <strong>Run larger experiments for analytical grade protein quantitation.</strong><br> With space for up to 96 unique samples and 4 molecular weight ladders per run, now you can run full standard curves, quality control (QC) samples, blanks, controls, references, and replicates alongside your unknown samples for robust analytical-grade quantitation of protein biomarker concentration and protein expression. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-2272024125313.png" data-srcset="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-2272024125313.png" alt="Design high specificity quantitative immunoassays, even in crude lysates and tissue homogenates." title="Design high specificity quantitative immunoassays, even in crude lysates and tissue homogenates." data-caption="Leo separates sample proteins by molecular weight before immunodetection, offering the ability to resolve non-specific binding and impart additional specificity in peak detection compared to other quantitative immunoassays that rely solely on immunodetection. After separation and immobilization of sample proteins, the sample matrix is flushed thoroughly from the capillary, so immunodetection occurs within an idealized buffer environment, minimizing cross-reactivity and non-specific binding for complex sample analysis." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Design high specificity quantitative immunoassays, even in crude lysates and tissue homogenates.</h4> <p> Leo separates sample proteins by molecular weight before immunodetection, offering the ability to resolve non-specific binding and impart additional specificity in peak detection compared to other quantitative immunoassays that rely solely on immunodetection. After separation and immobilization of sample proteins, the sample matrix is flushed thoroughly from the capillary, so immunodetection occurs within an idealized buffer environment, minimizing cross-reactivity and non-specific binding for complex sample analysis. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-2272024125359.png" data-srcset="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-2272024125359.png" alt="Quantify protein isoforms and size variants." title="Quantify protein isoforms and size variants." data-caption="Simple Western is the only quantitative immunoassay technology that adds electrophoretic separation of sample proteins before immunodetection, enabling quantification of protein isoforms that differ in size. Using Simple Western technology, isoforms can be separated by size and subsequently detected using pan-target antibodies that bind to all isoforms present in the sample. The Leo System provides robust quantitative immunoassays that distinguish intact vs cleaved isoforms, fragments, aggregates, degradation products, oligomers, and more." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Quantify protein isoforms and size variants.</h4> <p> Simple Western is the only quantitative immunoassay technology that adds electrophoretic separation of sample proteins before immunodetection, enabling quantification of protein isoforms that differ in size. Using Simple Western technology, isoforms can be separated by size and subsequently detected using pan-target antibodies that bind to all isoforms present in the sample. The Leo System provides robust quantitative immunoassays that distinguish intact vs cleaved isoforms, fragments, aggregates, degradation products, oligomers, and more. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-227202413353.png" data-srcset="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-227202413353.png" alt="Multiplexed protein detection and total protein normalization to maximize results from your precious sample." title="Get more results from your precious sample." data-caption="Detect up to 8 targets from just 3–5 µL of sample. Use the RePlex™ Module, an automated stripping and re-probing replacement, to run two immunoassays in series or run an immunoassay followed by total protein detection for more accurate protein expression normalization. Plus, resample up to 4 times from the same well for analysis in independent capillaries, thus avoiding any concerns about antibody cross reactivity." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Get more results from your precious sample.</h4> <p> Detect up to 8 targets from just 3–5 µL of sample. Use the RePlex™ Module, an automated stripping and re-probing replacement, to run two immunoassays in series or run an immunoassay followed by total protein detection for more accurate protein expression normalization. Plus, resample up to 4 times from the same well for analysis in independent capillaries, thus avoiding any concerns about antibody cross reactivity. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-237202417655.png" data-srcset="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-237202417655.png" alt="Faster immunoassay method development" title="Speed up your Simple Western method development and optimization." data-caption="Determine optimal assay conditions on the Leo System in as little as a day. You can test multiple sample concentrations, antibody titration series, and positive and negative controls in 100 independent capillaries in one run. In addition, Leo includes high dynamic range (HDR) detection, like Jess and Abby." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>Speed up your Simple Western method development and optimization.</h4> <p> Determine optimal assay conditions on the Leo System in as little as a day. You can test multiple sample concentrations, antibody titration series, and positive and negative controls in 100 independent capillaries in one run. In addition, Leo includes high dynamic range (HDR) detection, like Jess and Abby. </p> </td> </tr> <tr class="row m___product-data-examples"> <td class="border-0"> <div class="image-slider-container"> <div class="product-image-lightbox" data-brand=""> <div class="text-center border rounded p-2 item d-flex align-items-center justify-content-center bg-white active"> <img src="data:image/png;base64,R0lGODlhAQABAAD/ACwAAAAAAQABAAACADs=" data-src="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-2372024171048.png" data-srcset="https://resources.rndsystems.com/images/datasheets/004-550_leo-system-with-pc-simple-western-2372024171048.png" alt="One platform that meets your needs from discovery through analytical development and commercialization." title="One system that meets your needs from discovery through analytical development and commercialization." data-caption="Maintain flexibility in your assay design and throughput as you progress from discovery through IND and into commercialization. You have the option to run 1-4 cartridges (25-100 samples) per run and detect up to 8 targets per sample. Start developing your assay on the Leo System with one cartridge at a time and scale up to maximal throughput when needed. Leo allows you to design assays capable of relative or absolute protein quantitation. Save time by developing and scaling up on the same system, enabling you to reach your next milestone faster.<br><br> <strong>Confidence to meet regulatory guidelines for bioanalytical method validation.</strong><br> Compass™ Software for Simple Western™ Technology is the software package that all Simple Western instruments use to acquire, manage, and analyze data. Compass for Simple Western Software 7.0 has all the tools required for 21 CFR Part 11 compliance. Leo can boost your confidence to generate data that meets regulatory guidelines for bioanalytical method validation." data-badges="" data-application="Simple Western" width="250" height="250" class="search_result_thumbnail_image lazy" /> <div class="slider-open"> <div class="count-container text-center px-2 py-2 border-top border-left rounded-left"> <span class="icon-icon-image-camera-plus align-middle"></span> </div> </div> </div> </div> </div> </td> <td class="w-75 border-0 align-top"> <h4>One system that meets your needs from discovery through analytical development and commercialization.</h4> <p> Maintain flexibility in your assay design and throughput as you progress from discovery through IND and into commercialization. You have the option to run 1-4 cartridges (25-100 samples) per run and detect up to 8 targets per sample. Start developing your assay on the Leo System with one cartridge at a time and scale up to maximal throughput when needed. Leo allows you to design assays capable of relative or absolute protein quantitation. Save time by developing and scaling up on the same system, enabling you to reach your next milestone faster.<br><br> <strong>Confidence to meet regulatory guidelines for bioanalytical method validation.</strong><br> Compass™ Software for Simple Western™ Technology is the software package that all Simple Western instruments use to acquire, manage, and analyze data. Compass for Simple Western Software 7.0 has all the tools required for 21 CFR Part 11 compliance. Leo can boost your confidence to generate data that meets regulatory guidelines for bioanalytical method validation. </p> </td> </tr> </article> </table> </div> <div id="how_does_work-anchor" class="nav-link static-anchor p-0" data-anchor-label="How Does Leo System Work?"></div> <div id="applications-anchor" class="nav-link static-anchor p-0" data-anchor-label="Applications"></div> <div id="products_compatible-anchor" class="nav-link static-anchor p-0" data-anchor-label="Products Compatible with Leo System"></div> <div id="specifications-anchor" class="nav-link static-anchor p-0" data-anchor-label="Specifications"></div> <div id="product-datasheet-anchor" class="nav-link static-anchor p-0" data-anchor-label="Product Documents"></div> </div> </div> <div class="tab-pane fade mx-4 py-4" id="citations-tab" data-tab-label="Citations / Testimonials (32)" role="tabpanel" aria-labelledby="citations-tab-tab"><div class="product_details_anchors mb-2"> <div class="nav mx-0 pt-4 pb-2 m___nav-anchors d-none d-lg-flex"> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#citations"> Citations </a> </li> <li class="nav-item m-0"> <a class="nav-link jump-anchor active" href="#testimonials"> Testimonials </a> </li> </div> </div> <div id="citations"> <div class="pp_filter_list border-bottom mb-4 pb-3"> <h2>Citations for Leo System</h2> <p><a href="/resources/instrument-citations?instrument-platform=simple%20western" rel="nofollow noopener noreferrer" target="_blank">View all citations for the Simple Western platform.</a><br> Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments. </p> </div> </div> <div class="testimonials" id="testimonials"><article class="product-data-wrapper"> <div class="pp_filter_list mt-4 pt-4 border-bottom mb-4 pb-3"> <h2>Testimonials from your Peers for Simple Western</h2> <div class="testimonials_showing pp_filters_showing row col-md-12 mb-2"> <span class="less">Showing  <span class="testimonials_show_min font-weight-bold">1</span> <span class="font-weight-bold">-</span> <span class="testimonials_show_max font-weight-bold">5</span> of <span class="testimonials_count font-weight-bold">32</span> testimonials </span> <span class="more font-weight-bold">Showing All</span> </div> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/headshots/tatiana-rosenstock-headshotv2.png" alt="Tatiana Rosenstock, Principal Scientist, Sygnature Discovery" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Jess is faster and more reliable in terms of quantification and allows us to use low sample volumes, for example, difficult-to-obtain samples such as iPSC-derived neurons."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/tatiana-moves-closer-to-treating-neurodegenerative-diseases-with-jess">Tatiana Moves Closer to Treating Neurodegenerative Diseases With Jess</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Dr. Tatiana Rosado Rosenstock, </span> Principal Scientist, Sygnature Discovery </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/headshots/fyp-Chris-LaBreck-200x200.png" alt="Chris LaBreck, Ph.D., Senior Product Scientist, Cell Signaling Technology" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-cst-antibody-validation">Translating Traditional Western Blots to Simple Western is Fast and Easy</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Chris LaBreck, Ph.D., </span> Senior Product Scientist, Cell Signaling Technology (CST) </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/headshots/fpy-headshot-laura-martins-200x200.png" alt="Laura Martins Ph.D." loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Simple Western™ allows us to run three-hour assays, which would usually take us two days with traditional Western blots."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/advancing-cell-and-gene-therapies-with-simple-western">Advancing Cell and Gene Therapies with Jess</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Laura Martins, Ph.D., </span> Early Development Scientist, Oxford Biomedica </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/fyp_john_jess.png" alt="John Blackwood PhD Head of Biology at Samsara Therapeutics Headshot" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease"</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/restoring-autophagy-reversing-disease-with-jess">Restoring Autophagy, Reversing Disease with Jess </a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> John K. Blackwood PhD, </span> Head of Biology, and Jakub Stefaniak PhD, Samsara Therapeutics </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/fyp_mario_abby.png" alt="FYP Abby Competition Mario Tile" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The introduction of an Abby will enable us to answer important questions quicker, such as identifying the disease driving mechanism and molecules surrounding osteoarthritis. Moving away from the tedious manual western blotting process, which can take up to two days, and introducing automation will speed up time to data and provide increased sensitivity and accuracy."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/musculoskeletal-disease-researchers-utilitze-abby-for-protein-analysis">Accelerate Osteoarthritis Research Studies with Simple Western</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Dr. Mario Rothbauer, </span> Postdoc Researcher, Department of Orthopedics and Trauma Surgery, Medical University of Vienna </p> </section> </div> </div> </article> <span id="more-testimonials" style="display:none"> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-harte.jpg" alt="Dr Michael Harte tile" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"It can be difficult to optimize new antibodies, particularly for brain markers where you have a lot of background noise and low levels of your target. The appropriate control and low variability between plates using Simple Western™ means we can complete our studies in weeks rather than months."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/utilizing-simple-western-to-understand-neuronal-markers-schizophrenia">Utilizing Simple Western to Understand Neuronal Markers in Schizophrenia</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Dr. Michael Harte, </span> Senior Lecturer in Drug Action, Neuroscience, University of Manchester </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-noelle-anastasio.png" alt="Noelle Anastasio Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"One of the best advantages of using Wes is that it saves a lot of time, both in terms of hands-on time and in time to completion of the experiment. We were spending more than 4 hours of hands-on time per Western blot with traditional methods, and we can set up a run on the Wes in about 45 minutes. Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-changes-brain-receptor-research-game">Noelle Changes Her Brain Receptor Research Game with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Noelle Anastasio, Ph.D., </span> Assistant Professor, Department of Pharmacology and Toxicology, University of Texas Medical Branch </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-mary-howell.png" alt="Elizabeth Rick uses Luminex porcine assays to perform traumatic brain injury biomarker research" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"We collect samples from laser microdissection and were using our entire sample on just one regular Western blot. With Wes, we can do multiple assays with one sample collection."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-ups-data-point-count-on-laser-capture-microdissection-samples">Mary Ups Her Data Point Count on Laser Capture Microdissection Samples with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Mary Howell, </span> Laboratory Coordinator, Department of Internal Medicine, East Tennessee State University </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-miranda.png" alt="Miranda Orr Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The rapid, reproducible results on small amounts of tissue/cell lysates have allowed me to generate a more thorough data set on additional proteins, samples, conditions and brain regions all in the same amount, or even less time, as traditional Western blot. This technology has made it possible for small research labs to compete with the pace that research is conducted in large labs or companies."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-sets-a-new-pace-for-alzheimers-research">Miranda Sets a New Pace for Alzheimer's Research with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Miranda Orr, Ph.D., </span> Postdoctoral Fellow, University of Texas Health Science Center </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-brian-perrino.png" alt="Brian Perrino Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"We had been trying to study receptor proteins with traditional Westerns but it was frustrating—we just could not get it. A few months with Wes and we've already had way more success than we had in three years."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-cuts-time-to-results-and-solves-membrane-protein-challenges">Brian Cuts Time to Results and Solves His Membrane Protein Challenges with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Brian Perrino, Ph.D., </span> Associate Professor, Department of Physiology and Cell Biology, University of Nevada School of Medicine </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-jamie-boisvenue.png" alt="Jamie Boisvenue Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The traditional blotting method can use up to 300 pieces of plastic and [Simple Western] uses two. What's even better is that [Simple Western] can run up to 40 proteins in three days. It would take about a week to run 40 proteins using Western blotting."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-keeps-things-greener">Jamie Keeps Things Greener with Simple Western</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Jamie Boisvenue, </span> Cardiovascular Research Technician, Department of Pediatrics, University of Alberta Faculty of Medicine & Dentistry </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-mary-g-johlfs.png" alt="Mary G. Johlfs Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The Simple Western Charge assay, a capillary isoelectric focusing immunoassay, exceeded the reliability of 2D Western blots for resolving recombinant PKG-Iα and PKG-Iβ and uses 100,000X less sample quantity, making it well-suited for clinical disease proteomics because protein isoforms and post-translational modifications can be detected in precious tissue biopsies."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/nanopro-links-between-obesity-and-cancer">Distinguish PKG Antibody Isoforms with Simple Western</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Mary G. Johlfs, M.S., </span> Director of Research Operations/Scientist, Roseman University of Health Sciences </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-emma-wilson.png" alt="Emma Wilson" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Wes gives me more throughput so I can get more replicates and trust my results a little bit more, and I can trust them straight away rather than having to do a lot of repeats. And obviously I like getting results the same day rather than having to wait two days"</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-better-outcomes-for-osteoarthritis-patients">Emma Gets to Better Outcomes for Osteoarthritis Patients with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Emma Wilson (née Humphrey), Ph.D., </span> Lecturer in Molecular Biology, Institute of Medicine, University of Chester </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-uconn-Kazuyam.png" alt="Kazuya Machida thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The no-gel, no-transfer, no-membrane features are beneficial for the reproducibility of the assay. I really like this system over traditional Western."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/far-western-blotting-detect-protein-phosphorylation-with-simple-western-wes">Far Western Blot With Simple Western To Detect Protein Phosphorylation</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Kazuya Machida, M.D., Ph.D., </span> Associate Professor, Department of Genetics and Genome Sciences, UConn Health </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-melyssa-bratton.png" alt="Melyssa Bratton thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"We’ve been able to increase productivity dramatically with Wes. The results are much better too, given that it removes the blotting step all together."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-speeds-up-the-core">Melyssa Speeds Up the Core with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Melyssa Bratton, Ph.D., </span> Manager of the Cell, Molecular, and Biostatistics Core, Xavier University of Louisiana </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-jane.png" alt="Dr Jane Gray Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"[Simple Western] is easy to use, convenient, and gives answers quickly. The throughput and system automation is a definite advantage."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-gets-fast-answers-for-cancer-research">Make Quick Advances in Cancer Research with Simple Western</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Jane Gray, Ph.D., </span> Head of Research Instrumentation, Cancer Research UK Cambridge Institute </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-debbi-hicks.jpg" alt="Debbi Hicks Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Some of the proteins we wanted to detect are low in abundance and it’s really hard to get a good amount of protein for traditional Western blot. The low protein concentrations required by [Simple Western] makes it easier to save precious samples ensuring protein detection."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-detecting-low-abundance-proteins-in-precious-pediatric-cancer-samples">Detecting Low Abundant Proteins in Precious Pediatric Cancer Samples</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Debbie Hicks, Ph.D., </span> Faculty Fellow, Wolfson Childhood Cancer Research Centre, Northern Institute for Cancer Research, Newcastle University </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-irene-tobias.jpg" alt="Irene Tobias Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Wes allows us to measure cell signaling proteins from a single muscle fiber segment by lowering our limit of detection nearly ~20-fold from Western blotting. It also allows for much more precise, reproducible, and faster results to be generated, in addition to far greater ease of operation, analysis, and waste disposal."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-lower-limit-detection-advances-understanding-of-metabolic-signaling-in-human-muscle">Lower Limit of Detection Advances Understanding of Metabolic Signaling in Human Muscle</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Irene Tobias, Ph.D., </span> Postdoctoral Researcher, CSU Fullerton </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-y-senis.jpg" alt="Yotis Senis Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"[Wes] Provides quantitative data, saves time and uses far less sample."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-quantifying-protein-phosphorylation-in-platelets">Quantifying Protein Tyrosine Phosphatase in Platelets</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Yotis Senis, Ph.D., </span> Professor and Chair, University of Birmingham, UK </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-krishna.png" alt="Krishna C. Yalla" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"I was able to screen various antibodies in a single run within half a day. This had previously taken weeks and months using traditional Western blotting methods"</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-pharmacodynamic-biomarker-discovery">Wes Zips Through Pharmacodynamic Biomarker Discovery Projects at the University of Glasgow</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Krishna C. Yalla, Ph.D., </span> Postdoctoral Research Scientist, Institute of Cancer Sciences, University of Glasgow </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-j-voutila.png" alt="Jon Voutila Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"I can run immunoassays on Wes for an entire animal experiment in a single day. This is something I wouldn’t even try with traditional Western blots."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/simple-western-wes-validates-RNA-based-metabolic-therapies-potential">Wes Validates the Potential of RNA-based Metabolic Therapies At MiNA Therapeutics</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Jon Voutila, Ph.D., </span> Senior Research Scientist, MiNA Therapeutics, London, UK </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-ferrari.png" alt="Dr Ferrari Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Simple Western allowed me to screen quickly lots of samples with different antibodies while eliminating the manual washing and incubation steps."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/jess-simple-western-coronavirus-vaccine">Simple Western and the Race to Develop a Vaccine for the COVID-19 Coronavirus</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Dr. Matteo Ferrari, </span> Postdoc, DIOSynVax, Department of Veterinary Medicine, Cambridge, United Kingdom </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-volta.png" alt="Dr. Mattia Volta thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"It’s had a significant impact on our research... we get double the amount of data in a third of the time."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/researching-origins-of-parkinsons-disease-with-simple-western">Rebel without a Cause - Searching for the Origins of Parkinson's Disease with Wes</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Dr. Mattia Volta, </span> Senior Researcher, EURAC Research </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-rachel-doidge.png" alt="Rachel Doidge thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"I work for a fast-paced drug discovery CRO where our clients expect high-quality data with rapid turnarounds. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/protac-drug-discovery-jess">PROTAC Studies Take Off in Drug Discovery, and Jess is on Board</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Rachel Doidge Ph.D, </span> Senior Research Scientist, Aurelia Bioscience, Biocity, Nottingham, UK </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-tonmoy-sarkar.png" alt="Tonmoy Sarkar Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"The quantitative assays and short run times on Jess enable us to generate data faster and be more confident in our results."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/jess-screens-new-chemical-entities-at-tcg-lifesciences">Jess Breaks New Quantitative Ground in New Chemical Entity Screening at TCG Lifesciences</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Tonmoy Sarkar, Ph.D., </span> Senior Research Scientist, TCG Lifesciences </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-kamarudin.png" alt="Muhumad Noor Alfarizal Kamarudin thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Jess allows me to reduce the preparation time, save on reagents, samples, and antibodies, while allowing easier optimization of target proteins/antibodies. Jess’s ability to detect multiple proteins per sample further saves time compared to conventional Western blot."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/jess-finds-novel-therapeutics-for-glioma">Conducting Novel Therapeutics Research of Human Gliomas with Jess</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Muhumad Noor Alfarizal Kamarudin, </span> Research Fellow, Brain Research Institute Monash Sunway (BRIMS) </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-nicolas-g-bazan.png" alt="Nicolas Bazan thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"It saves so much time! It takes away so much of the pipetting and manipulation error. You just load your samples and wait for results, and in the meantime, you can dedicate yourself to other activities."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/unraveling-neurodegenerative-diseases-with-jess">Unraveling Neurodegenerative Diseases with Jess</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Nicolas G. Bazan, MD, PhD, </span> Director of Neuroscience Center of Excellence, School of Medicine, Louisiana State University Health New Orleans, New Orleans </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-joe-klebba.png" alt="Joe Klebba Thumbnail" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"By switching my assays over to Simple Western, I am able to increase my throughput, allowing for full dose responses to be generated in each experiment. Utilizing the RePlex module even further increases this throughput as I often probe for 5-6 proteins per capillary in a single run including phosphorylated/total protein pairs in the same lane."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/targeting-once-inaccessible-phospho-proteins-with-replex">Detect Phosphorylation of Proteins with RePlex</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Joseph Klebba, Ph.D., </span> Scientist, Vividion Therapeutics </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-elena-navarro-guerrero.jpg" alt="Elena Navarro-Guerrero" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"I used Simple Western because the protein samples I had were small. We only had a small number of [iPSC-derived macrophages] because the cell line does not proliferate."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/elena-unlocks-immune-cell-potential-simple-western-crispr-cas9">Elena Unlocks Immune Cell Potential with Simple Western and CRISPR/Cas9</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Elena Navarro-Guerrero, Ph.D., </span> Senior Postdoctoral Researcher. Nuffield Department of Medicine, Target Discovery Institute </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-muhammad-dain-yazid.png" alt="Muhammad Dain Yazid" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Protein analysis using [Simple Western] can be performed faster, eliminating tedious work and saving time."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/muhammad-simple-western-chart-signaling-pathways-stem-cells">Muhammad Uses Simple Western to Chart Stem Cell Signaling Pathways</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Muhammad Dain Yazid, Ph.D., </span> Centre for Tissue Engineering & Regenerative Medicine, University Kebangsaan Malaysia (UKM) Medical Centre </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-kristine-nolling-jensen.png" alt="Kirstine Nolling Jensen, PhD Student" loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Jess is a user-friendly, multi-purpose platform that gives me the possibility to perform Western blot analysis on precious samples, as well as quantifying proteins expressed in the cells at the same time."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/kirstine-nolling-jensen-Investigating-resolution-inflammation-jess">Investigating Resolution of Inflammation with Jess</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Kirstine Nolling Jensen, PhD Student, </span> Department of Immunology, Landspitali, the National University Hospital Iceland </p> </section> </div> </div> </article> <article role="article" class="contextual-region"> <div class="testimonial py-4"> <div class="row"> <aside class="col-lg-2 m___testimonial-aside-search text-center"> <article class="contextual-region"> <img src="https://resources.bio-techne.com/bio-techne-assets/images/testimonials/simple-western-nageswara-karolinska.png" alt="Nageswara Rao Boggavarapu, PhD." loading="lazy"> </article> </aside> <section class="col-lg-10"> <div class="testimonial__quote"> <p>"Our lab came across ProteinSimple’s Simple Western platforms in 2014 and was surprised by the sensitivity and specificity of the technology, as well as its minimal usage of consumables."</p> </div> <div class="testimonial-link"> <a href="/resources/testimonials/nageswara-complexities-of-endometrial-receptivity-with-simple-western">Delving into the Complexities of Endometrial Receptivity with Simple Western</a> </div> <p class="testimonial__profile mt-3"> <span class="font-weight-bold"> Nageswara Rao Boggavarapu, PhD, </span> Assistant Professor, Women’s and Children’s Health, Karolinska Institute, Stockholm, Sweden </p> </section> </div> </div> </article> </span> <div id="testimonial_counts" data-max="32" data-min="5"> <div class="testimonials_showing pp_filters_showing row col-md-12 mb-2"> <span class="less">Showing  <span class="testimonials_show_min font-weight-bold">1</span> <span class="font-weight-bold">-</span> <span class="testimonials_show_max font-weight-bold">5</span> of <span class="testimonials_count font-weight-bold">32</span> testimonials </span> <span class="more font-weight-bold">Showing All</span> </div> </div> <div class="text-center"> <button id="testimonials-view-more" class="btn-secondary">View More</button> </div> </div> </article> </div> </div> </div> </div> <div><div id="accordion" class="border mb-4"> <div class="rounded-top bg-white"> <h3 class="m-0 p-3">More Information About Leo System</h3> </div> <div class="vtab-section" data-section="How Does Leo System Work?" id="how-does-leo-system-work"> <div class="card-header rounded-top"> <a id="how-does-leo-system-work-link" class="collapsed" data-toggle="collapse" data-target="#collapse_region-5781592502255" aria-expanded="false" aria-controls="collapse_region-5781592502255"> <h5 class="mb-0"> How Does Leo System Work? </h5> </a> </div> <div id="collapse_region-5781592502255" class="collapse " aria-labelledby="heading_region-5781592502255" data-parent="#accordion"> <div class="card-body"> <div id="how-does-leo-system-work-vtab" data-tab-label="How Does Leo System Work?" role="tabpanel" aria-labelledby="how-does-leo-system-work-vtab-tab"><div id="instrument_how_work_content"> <div class="paragraph 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href="/search?keywords=Simple%20Western%20Assay%20Modules&sub_category=Simple%20Western%20Assay%20Modules&compatible_instrument=Leo&view=condensed">Simple Western Assay Modules</a></li> <li class="py-2"><a href="/search?keywords=Simple%20Western%20Consumables%20for%20Lot%20Controlled%20Orders&sub_category=Simple%20Western%20Consumables%20for%20Lot%20Controlled%20Orders&compatible_instrument=Leo&view=condensed">Simple Western Consumables for Lot Controlled Orders</a></li> <li class="py-2"><a href="/search?keywords=simple%20western&compatible_instrument=Leo&view=condensed">View All Products Compatible with Leo</a></li> </ul> </article> </div> </div> </div> </div> </div> <div class="vtab-section" data-section="Specifications" id="specifications"> <div class="card-header rounded-top"> <a id="specifications-link" class="collapsed" data-toggle="collapse" data-target="#collapse_region-9701592502350" aria-expanded="false" aria-controls="collapse_region-9701592502350"> <h5 class="mb-0"> Specifications </h5> </a> </div> <div id="collapse_region-9701592502350" class="collapse " aria-labelledby="heading_region-9701592502350" data-parent="#accordion"> <div class="card-body"> <div id="specifications-vtab" data-tab-label="Specifications" role="tabpanel" aria-labelledby="specifications-vtab-tab"><div id="instrument_specifications"><table class="tablesaw tablesaw-stack app_table my-3 table-bordered" data-tablesaw-mode="stack"> <thead class="ds_table_head bg-light-gray"> <th class="ds_table_head_cell w-25 p-3">Description</th> <th class="ds_table_head_cell w-25 p-3">Leo System </th> </thead> <tbody class="bg-white"> <tr class="ds_table_row"> <td>Max samples per run </td> <td colspan="1" class="text-left"> 100 </td> </tr> <tr class="ds_table_row"> <td>Run time </td> <td colspan="1" class="text-left"> 3 hours <br /> or <br /> 5 hours with RePlex </td> </tr> <tr class="ds_table_row"> <td>Minimal sample volume required </td> <td colspan="1" class="text-left"> 3-5 μL/well </td> </tr> <tr class="ds_table_row"> <td>Detection </td> <td colspan="1" class="text-left"> Chemiluminescence </td> </tr> <tr class="ds_table_row"> <td>RePlex™ (Strip & ReProbe Replacement) </td> <td colspan="1" class="text-left"> Yes </td> </tr> <tr class="ds_table_row"> <td>Standard Fluorescence (NIR/IR) </td> <td colspan="1" class="text-left"> No </td> </tr> <tr class="ds_table_row"> <td>Stellar™ (NIR / IR fluorescence detection) </td> <td colspan="1" class="text-left"> No </td> </tr> <tr class="ds_table_row"> <td>Dimensions (H x W x D) </td> <td colspan="1" class="text-left"> 72 x 74 x 74 cm / 28.3 x 29.1 x 29.1 inch </td> </tr> <tr class="ds_table_row"> <td>Weight </td> <td colspan="1" class="text-left"> 136 kg / 300 lbs </td> </tr> </tbody> </table> </div> </div> </div> </div> </div> </div> </div> <div id="product-documents"> </div> <div id="product-page-details" data-time-Total="0" data-time-ToCompile="0" data-time-ToBuild="0" data-time-Datapoints="0" data-time-Template="0" 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