<?xml version="1.0" encoding="UTF-8"?> <article key="pdf/10008143" mdate="2017-09-01 00:00:00"> <author>Ahmad Manbohi and Seyyed Hamid Ahmadi</author> <title>Microfluidic PaperBased Electrochemical Biosensor</title> <pages>1781 - 1784</pages> <year>2017</year> <volume>11</volume> <number>11</number> <journal>International Journal of Mechanical and Mechatronics Engineering</journal> <ee>https://publications.waset.org/pdf/10008143</ee> <url>https://publications.waset.org/vol/131</url> <publisher>World Academy of Science, Engineering and Technology</publisher> <abstract>A lowcost paperbased microfluidic device (PAD) for the multiplex electrochemical determination of glucose, uric acid, and dopamine in biological fluids was developed. Using wax printing, PAD containing a central zone, six channels, and six detection zones was fabricated, and the electrodes were printed on detection zones using premade electrodes template. For each analyte, two detection zones were used. The carbon working electrode was coated with chitosanBSA (and enzymes for glucose and uric acid). To detect glucose and uric acid, enzymatic reactions were employed. These reactions involve enzymecatalyzed redox reactions of the analytes and produce free electrons for electrochemical measurement. Calibration curves were linear (R&amp;amp;sup2; &amp;amp;gt; 0.980) in the range of 080 mM for glucose, 0.09&amp;amp;ndash;0.9 mM for dopamine, and 0&amp;amp;ndash;50 mM for uric acid, respectively. Blood samples were successfully analyzed by the proposed method. </abstract> <index>Open Science Index 131, 2017</index> </article>