Frequently Asked Questions | Cell Signaling Technology

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<li>Frequently Asked Questions</li> </ol> </nav> <div data-template="content-page-header"> <h1>Frequently Asked Questions</h1> </div> </div> </div> <div class="row" data-template="subcategory"> <div class="col-md-8"> <div data-template="content-html-block"> Here are answers to the most frequently asked questions</p> <p>If for any reason you can't find the answer you're looking for, please call us at <span class="hidden" data-country="cn">4006-473287</span><span class="hidden" data-country="hk">(852) 2896-6308</span><span class="hidden" data-country="be">0800 71640</span><span class="hidden" data-country="de">+49 (0)69 9675 9070</span><span class="hidden" data-country="at">+43 (0)720 880 536</span><span class="hidden" data-country="gb">+44 1223 93 1164</span><span class="hidden" data-country="jp">03 (3295) 1632</span><span class="hidden" data-country="eu">+31 (0)71 7200 200</span><span class="hidden" data-country="default">+1-877-678-8324</span>.<div class="showMoreWrapper"> <a 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Each <a href="/about-us/our-approach-process/stability-test-results">CST antibody is stability tested</a> by exposure to room temperature and 37°C for one week followed by western blot testing to ensure no change in antibody performance. If you received an antibody at room temperature, rest assured that it has <a href="/about-us/our-approach-process/stability-test-results">passed stability testing</a> and that the antibody performance is still optimal. Upon receipt, please store the antibody as indicated on the product datasheet. </div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading1"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse1" aria-expanded="false" aria-controls="collapse1"> Q: How should I store my antibody tube? How much does temperature affect stability?</a> </h4> </div> <div id="collapse1" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading1"> <div class="panel-body"> A: Please store the antibodies in the original tube at -20°C and do not aliquot as this may introduce variables and waste antibody. The antibodies are provided in a buffer that contains 50% glycerol and do not freeze under the recommended storage conditions. This prevents degradation of the antibody due to repeated freeze-thaw cycles. Conjugated antibodies may indicate storage at 4°C, please store as suggested on the product datasheet. </div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading2"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse2" aria-expanded="false" aria-controls="collapse2"> Q: Have species been tested if they are not listed as cross-reactive with a CST antibody on the datasheet?</a> </h4> </div> <div id="collapse2" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading2"> <div class="panel-body"> A: Possibly. CST scientists test all CST antibodies on multiple species, if possible (this depends on the target and the experimental systems available). If tested species do not cross-react, we do not list that species as cross-reactive. However, it is possible that the model we test may contain a low level of particular protein or the inductions we used are not ideal in that particular model system. If you need additional information regarding cross-reactivity with a particular species, please feel free to <a href="/learn-and-support/order-support#tech-support">contact the CST technical support team</a> for assistance.</div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading3"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse3" aria-expanded="false" aria-controls="collapse3"> Q: Why can't I use my own western blotting protocol with CST antibodies?</a> </h4> </div> <div id="collapse3" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading3"> <div class="panel-body"> A: CST antibodies are optimized to provide the strongest signal with the least background, using our standard western blotting protocol. Using the recommended, optimized CST protocol saves you costly time and materials.</div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading4"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse4" aria-expanded="false" aria-controls="collapse4"> Q: Does this antibody work for my application (western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, flow cytometry, chromatin immunoprecipitation)?</a> </h4> </div> <div id="collapse4" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading4"> <div class="panel-body"> A: CST routinely tests antibodies in relevant applications. Those that pass rigorous application-specific validation standards are recommended. We only recommend applications that have passed rigorous validation standards, if performance data is ambiguous we do not recommend the application. Please <a href="/learn-and-support/order-support#tech-support">contact the CST technical support team</a> for more information about a particular antibody, or for a recommendation on which antibody will perform best in your desired application.</div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading5"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse5" aria-expanded="false" aria-controls="collapse5"> Q: Does this antibody work for fluorescent western blotting?</a> </h4> </div> <div id="collapse5" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading5"> <div class="panel-body"> <p> A: CST does not routinely test antibodies for fluorescent western blotting, though many work well in this application. Performing a fluorescent western blot using CST antibodies requires a modified immunoblotting protocol. Full details can be found in our <a href="/learn-and-support/protocols/protocol-western-fluorescent">Western Blotting Protocol (Fluorescent)</a>. </p></div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading6"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse6" aria-expanded="false" aria-controls="collapse6"> Q: When there are multiple antibody choices, which will work optimally for my application?</a> </h4> </div> <div id="collapse6" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading6"> <div class="panel-body"> A: Often, CST offers more than one antibody against a target. To choose the antibody that will perform the best in your desired application, please <a href="/learn-and-support/order-support#tech-support">contact CST technical support</a> for assistance in selecting the optimal antibody for your application.</div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading7"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse7" aria-expanded="false" aria-controls="collapse7"> Q: How does CST ensure the quality of their antibodies?</a> </h4> </div> <div id="collapse7" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading7"> <div class="panel-body"> A: CST scientists test all our products in relevant applications such as western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, flow cytometry, and chromatin immunoprecipitation. When an antibody is recommend for a particular application, it indicates that the antibody has passed rigorous application-specific testing standards. Additionally, our scientists create specialized (optimized) immunostaining protocols for individual products, saving you time and reagents. We routinely test our antibodies on multiple species including human, monkey, mouse, and rat. The high quality of our products is the most important consideration at CST. If you are interested in a particular application not listed on the product webpage, please <a href="/learn-and-support/order-support#tech-support">contact CST technical support</a> for further information. </div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading8"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse8" aria-expanded="false" aria-controls="collapse8"> Q: How does CST quality control for different lots?</a> </h4> </div> <div id="collapse8" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading8"> <div class="panel-body"> A: CST scientists strive to achieve product consistency from lot to lot. As a standard, we routinely test the new lot side-by-side with the old lot in multiple assays, to ensure equivalent activity. The recommended dilution ranges for each application are optimized for each product following our protocols.</div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading9"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse9" aria-expanded="false" aria-controls="collapse9"> Q: What is the concentration of my antibody?</a> </h4> </div> <div id="collapse9" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading9"> <div class="panel-body"> A: At CST, we formulate antibodies based on activity to eliminate the necessity for you to make adjustments. Concentrations between lots of antibodies are sometimes adjusted to optimize results. Please visit our <a href="/learn-and-support/technical-support/technical-documentation">Technical Documentation page</a> if you are looking for the concentration of a recently purchased antibody, Certificates of Analysis for the current shipping lot of many of our products and more. For the concentration of a previous antibody lot, please <a href="/learn-and-support/order-support#tech-support">contact CST technical support</a>. </div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading10"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse10" aria-expanded="false" aria-controls="collapse10"> Q: What is the shelf-life of my CST product?</a> </h4> </div> <div id="collapse10" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading10"> <div class="panel-body"> <p> A: When stored under the recommended conditions, CST products can be expected to perform at optimal levels during the specified shelf-life period. </p> <p> The product you receive will have one of two dates listed on its packaging. If the date is preceded by “Ref:”, then it is a “Reference Date”. This date indicates the date (month and year) that the product was filled or packaged. For single component products such as individual antibodies, the starting point for the expected shelf-life is indicated by the reference date printed on the vial label. For multi-component kit products the reference date printed on the barcoded kit box label indicates the starting point for the expected shelf-life. </p> <p> If the date is preceded by “Best:”, then it is a “Best By” date. This date indicates the end point of the shelf life of the product. Please expect the product to perform at optimal levels until this date, as long as it is stored under the recommended conditions. View our <a href="/learn-and-support/technical-support/product-shelf-life">list of shelf-life information by product type</a>. </p></div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading11"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse11" aria-expanded="false" aria-controls="collapse11"> Q: Can I order a custom formulation of this antibody?</a> </h4> </div> <div id="collapse11" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading11"> <div class="panel-body"> A: For custom (carrier-free) orders, many antibodies prepared formulated in PBS without BSA or glycerol are in stock. This formulation is amenable to conjugation and can be conjugated to fluorophores, beads, or dyes. In order to meet the requirements of your assay or your laboratory, we are also happy to provide you with bulk sales. Learn more about <a href="/services/carrier-free-and-customized-formulations">custom carrier-free and customized formulations</a> at CST.</div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading12"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse12" aria-expanded="false" aria-controls="collapse12"> Q: Could CST develop an antibody for me?</a> </h4> </div> <div id="collapse12" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading12"> <div class="panel-body"> A: CST does not develop custom antibodies, but we are happy to accept your target suggestions. Please <a href="/learn-and-support/order-support#product-suggestion">contact us with your product suggestion</a> and a CST scientist will contact you shortly.</div> </div> </div> <div class="panel panel-default" data-template="content-center-accordians"> <div class="panel-heading" role="tab" id="heading13"> <h4 class="panel-title"> <a role="button" data-toggle="collapse" href="#collapse13" aria-expanded="false" aria-controls="collapse13"> Q: Need further help?</a> </h4> </div> <div id="collapse13" class="panel-collapse collapse" role="tabpanel" aria-labelledby="heading13"> <div class="panel-body"> A: Please choose the appropriate form on our <a href="/learn-and-support/order-support">Order Support page</a>.</div> </div> </div> </div> </div> <div class="col-md-4"> <div data-template="content-related-pages"> <h2>Resources</h2> <ul class="subcategory-related-links" data-elastic-exclude> <li> <a href="/learn-and-support/protocols">Protocols</a> </li> <li> <a 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