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'' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); 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document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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class="parsys"><div class="text parbase section"><div class="table-component-wrapper"><table style="width: 100%;" id="table-1479254237"><tbody><tr><td style="vertical-align: middle; height: 250px;"><h1 style="color: #fff;">PCR Enzymes and Master Mixes</h1> </td></tr></tbody></table></div></div></div></div></div></div></div></div></div></div></div><div class="container page-header-hidden"><div class="row"><div class="span12"><div class="pageheading"></div></div></div></div><div class="container-wrap template-2-column"><div class="container"><div class="row"><script> var showHideText = { 'seeRelatedText':'See Navigation', 'hideRelatedText':'Hide Navigation' }; </script><div class="span3 sidebar" role="complementary"><div id="link-leftnav-toggle-container"><a class='link-leftnav-toggle' href='#'>See Navigation</a></div><div class="container-leftnav"><div class="railInheritParsys iparsys parsys"><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> &nbsp <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes.html"><b>PCR Enzymes and Master Mixes</b></a></li><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/pcr-enzymes-selection-tool.html">Enzyme selection tool</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Hot-start PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html">Platinum II <i>Taq</i> Hot-Start DNA polymerase</a></li><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/amplitaq-gold-dna-polymerases.html">AmpliTaq Gold DNA polymerase</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> High-fidelity PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase</a></li><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/accuprime-dna-polymerases.html">AccuPrime <i>Taq</i> DNA Polymerase High Fidelity</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Long-range PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase </a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> GC-rich PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase</a></li><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html">Platinum II <i>Taq</i> DNA Polymerase</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Multiplex PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase </a></li><li><a href="/order/catalog/product/4464270">Platinum Multiplex PCR Master Mix</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Direct PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html">Platinum Direct PCR Universal Master Mix</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Standard PCR enzymes <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/invitrogen-taq-dna-polymerases.html"><i>Taq</i> DNA polymerase</a></li><li><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/amplitaq-dna-polymerases.html">AmpliTaq DNA polymerase</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Resources <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/pcr-education.html">PCR education</a></li><li><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/tm-calculator.html">T<sub>m</sub> calculator</a></li><li><a href="/sg/en/home/brands/invitrogen/molecular-biology-technologies/pcr-video-library.html#pcr.html">PCR videos</a></li><li><a href="/sg/en/home/brands/invitrogen/molecular-biology-technologies/pcr-literature-resource-library.html?open=pcr#.html">PCR app notes</a></li><li><a href="/sg/en/home/brands/invitrogen/molecular-biology-technologies/pcr-webinars.html?open=pcr#.html">PCR webinars</a></li><li><a href="/sg/en/home/about-us/partnering-licensing/oem-commercial-supply/life-science-tools.html">OEM & customized PCR solutions</a></li></ul></li></ul></div></div><div class="leftnav section"><div class="leftnav-manual"><ul class="nav nav-list"><li class="item-header nav-header"> Related products <span class="item-icon icon-plus pull-right visible-sm visible-xs"></span></li><li class="item-content collapse" ><ul><li><a href="/sg/en/home/life-science/oligonucleotides-primers-probes-genes/custom-dna-oligos.html">PCR primers</a></li><li><a href="/sg/en/home/life-science/oligonucleotides-primers-probes-genes/nucleotides.html">dNTPs</a></li><li><a href="/sg/en/home/life-science/pcr/thermal-cyclers-realtime-instruments.html">Thermal cyclers & real-time PCR instruments</a></li><li><a href="/sg/en/home/life-science/pcr/reverse-transcription.html">Reverse transcription</a></li><li><a href="/sg/en/home/life-science/pcr/real-time-pcr.html">Real-Time PCR (qPCR)</a></li><li><a href="/sg/en/home/life-science/pcr/digital-pcr.html">Digital PCR</a></li><li><a href="/sg/en/home/life-science/pcr/pcr-plastics.html">PCR tubes, plates & accessories</a></li></ul></li></ul></div></div><div class="section"><div class="new"></div></div><div class="iparys_inherited"><div class="railInheritParsys iparsys parsys"></div></div></div><div class="railParsys parsys"></div></div></div><div class="span9 main" role="main"><div class="MainParsys parsys"><div class="text parbase section"><p>Choose from a variety of stand-alone PCR enzymes and master mixes optimized for your applications and experimental needs. </p></div><div class="heading section"><h2 id="top"> PCR master mix </h2></div><div class="text parbase section"><p>Our new generation of Platinum DNA polymerases come with novel buffers enabling universal <a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">primer annealing at a temperature of 60°C</a>. The universal primer annealing allows all primers to anneal to the template DNA at 60°C, eliminating T<sub>m</sub> calculations and enhancing convenience. A combination of the innovative buffers, high-performing enzymes, and reliable hot-start technology offers exceptional PCR results, even in the toughest applications.</p><p>A PCR master mix is a pre-mixed solution that contains a thermostable DNA polymerase, dNTPs (deoxynucleotide triphosphates), buffer, and other additives. The optimized master mix formulation helps ensure consistent and reliable results, save time, and reduce the potential for errors during reaction set up. In addition, PCR master mixes can be an economical choice compared to purchasing individual enzymes and reagents.</p></div><div class="text parbase section"><div class="alert alert-info"><b><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/pcr-enzymes-selection-tool.html#!/">Interactive enzyme selection tool</a></b>: Quickly find the DNA polymerase you need for your PCR! </div></div><div class="heading section"><h2 id="compare"> Compare PCR enzyme and master mix by PCR type </h2></div><div class="tabworkflowcontainer section"><div class="tabbable plain-tab"><ul class="nav nav-tabs" id="myTab"><li class="active"><a data-toggle="tab" href="#container-6cb12e16-4623-4d38-97b2-f8822de98181">Hot-start PCR</a></li><li><a data-toggle="tab" href="#container-a0c70367-ad37-4d5c-9ce2-dd1bd1f0a536">High-fidelity PCR</a></li><li><a data-toggle="tab" href="#container-bf65cc05-e4a5-49fe-8385-fefb0c8d52b9">Long-range PCR</a></li><li><a data-toggle="tab" href="#container-9f90d0a8-fc44-47ed-92d9-9e83f7c3b7b0">Multiplex PCR</a></li><li><a data-toggle="tab" href="#container-e7a1f667-1499-47ad-a376-041f895c39b0">GC-rich PCR</a></li><li><a data-toggle="tab" href="#container-7cac2fd4-b87f-43fd-88a3-3d461299747d">Direct PCR</a></li></ul><div class="tab-content" id="myTabContent"><div id="container-6cb12e16-4623-4d38-97b2-f8822de98181" class="tab-pane active"><div class="container-6cb12e16-4623-4d38-97b2-f8822de98181 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Hot-start PCR </h3></div><div class="text parbase section"><p>Hot-start PCR is a type of PCR where DNA polymerase activity is inhibited until the initial denaturation step. This inhibition is achieved through various methods, such as antibodies, affibodies, or chemical modifications of the polymerase. The benefits of hot-start PCR include increased specificity and yield.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--700275886"><tbody><tr><th width="15%"> </th><th style="text-align: center;" width="25%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-II-Taq-Hot-Start-Polymerase.jpg" width="70%" title="Platinum II Taq Hot-start DNA Polymerase" alt="product package and three tubes of reagents"><br></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html">Platinum II <i>Taq </i>Hot-Start DNA Polymerase</a><br></p></th><th style="text-align: center;" width="25%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/amplitaq-gold-dna-polymerases.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/AmpliTaq-Gold-360.jpg" width="70%" title="AmpliTaq Gold 360 DNA Polymerase" alt="product package and four tubes of reagents"><br></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/amplitaq-gold-dna-polymerases.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/amplitaq-gold-dna-polymerases.html">AmpliTaq Gold 360 DNA Polymerase</a><br></p></th></tr><tr><td><span class="side"><b>Hot-start technology</b></span></td><td style="text-align: center;">Antibody</td><td style="text-align: center;">Chemical</td></tr><tr><td><span class="side"><b>Enzyme activation time</b></span></td><td style="text-align: center;">2 min</td><td style="text-align: center;">10 min</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>DNA synthesis speed</b></td><td style="text-align: center;">15 sec/kb</td><td style="text-align: center;">60 sec/kb</td></tr><tr><td><b>Flexible extension step*</b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>Inhibitor tolerance</b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;">Up to 5 kb</td><td style="text-align: center;">Up to 5 kb</td></tr><tr><td><span class="side"><b>GC-rich format</b></span></td><td class="check-cvc" style="text-align: center;">Yes</td><td class="check-cvc" style="text-align: center;">Yes</td></tr><tr><td><span class="side"><b>Stand-alone enzyme</b></span></td><td style="text-align: center;">Colorless†<br><a href="/order/catalog/product/14966001"><span class="btn btn-primary">Order now</span></a></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/4398813"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td><span class="side"><b>Master mix format</b></span></td><td style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/14000012"><span class="btn btn-primary">Order now</span></a></p><p>Green**<br><a href="/order/catalog/product/14001012"><span class="btn btn-primary">Order now</span></a></p></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/4398881"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td colspan="3"><small>*The extension step can be extended up to 60 sec/kb without the effect of specificity.<br> **Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.<br> †<a href="/order/catalog/product/14966123">Green buffer</a> available as separate item for use with stand-alone enzyme.</small></td></tr></tbody></table></div><div class="alert alert-info"><p><b>Note</b>: The original Platinum <i>Taq</i> DNA Polymerase is available in the formats of <a href="/order/catalog/product/10966018">stand alone</a>, <a href="/order/catalog/product/11966034">stand alone with gel-loading dyes</a>, <a href="/order/catalog/product/13000012">master mix</a>, and <a href="/order/catalog/product/13001012">master mix with gel-loading dyes</a>.<br></p></div></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainercontainer-6cb12e16-4623-4d38-97b2-f8822de98181tabwell_a5f2uipartextimage_929c"><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/platinum-dna-polymerases-brochure.pdf" target="_blank"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/tabworkflowcontainer/container-6cb12e16-4623-4d38-97b2-f8822de98181/tab/well_a5f2/uipar/textimage_929c/image.img.full.high.jpg/1730321144922.jpg' media="(min-width: 1px)"><img 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alt='Platinum DNA polymerases brochure thumbnail' title='A hot start towards your PCR destinations'></picture></div><style> #cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainercontainer-6cb12e16-4623-4d38-97b2-f8822de98181tabwell_a5f2uipartextimage_929c { max-width: 100%; } #cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainercontainer-6cb12e16-4623-4d38-97b2-f8822de98181tabwell_a5f2uipartextimage_929c img { width: 100%; max-width: 100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h2>A hot start towards your PCR destinations</h2><p>Find comparison of technical specifications between Platinum II Taq Hot-Start and Platinum Taq DNA Polymerases</p><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/platinum-dna-polymerases-brochure.pdf" target="_blank" class="btn btn-primary" adhocenable="false">Download the brochure</a></p></div></div><div class="clearfix"></div></div></div></div></div></div></div></div><div class="end"></div></div></div><div id="container-a0c70367-ad37-4d5c-9ce2-dd1bd1f0a536" class="tab-pane"><div class="container-a0c70367-ad37-4d5c-9ce2-dd1bd1f0a536 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> High-fidelity PCR </h3></div><div class="text parbase section"><p>High fidelity PCR, also known as proofreading PCR, is a technique that utilizes DNA polymerases with a built-in proofreading capability to ensure accurate sequence amplification with no errors introduced in the sequence. </p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--510242724"><tbody><tr><th width="15%"> </th><th style="text-align: center;" width="33%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-SuperFi-II.jpg" width="70%" title="Platinum SuperFi II DNA Polymerase" alt="Product package and three tubes of reagents"></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><span>Platinum SuperFi II DNA Polymerase</span></a><br></p></th><th style="text-align: center;" width="33%"><p><a href="/order/catalog/product/11304011"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-II-Taq-Hot-Start-DNA-Polymerase.jpg" width="70%" title="Platinum II Taq Hot-start DNA Polymerase" alt="Product package and three tubes of reagents"></a></p><a href="/order/catalog/product/11304011"></a><p><a href="/order/catalog/product/11304011"><span>Platinum <i>Taq</i> DNA Polymerase High Fidelity</span></a><br></p></th></tr><tr><td><span class="side"><b>Fidelity vs. <i>Taq</i> enzyme</b></span></td><td style="text-align: center;">>300x</td><td style="text-align: center;">6x</td></tr><tr><td><span class="side"><b>Hot start for enhanced specificity</b></span></td><td class="check-cvc" style="text-align: center;">Yes</td><td class="check-cvc" style="text-align: center;">Yes</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;">Up to 20 kb*</td><td style="text-align: center;">Up to 20 kb</td></tr><tr><td><b>Amplicon overhangs</b></td><td class="check-cvc" style="text-align: center;">Blunt</td><td style="text-align: center;">3′ A/Blunt<br></td></tr><tr><td><span class="side"><b>GC-rich amplification</b></span></td><td class="check-cvc" style="text-align: center;">Yes<br></td><td style="text-align: center;">No</td></tr><tr><td><span class="side"><b>Stand-alone enzyme</b></span></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/12361010"><span class="btn btn-primary">Order now</span></a></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/11304011"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td><span class="side"><b>Master mix format</b></span></td><td style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/12368010"><span class="btn btn-primary">Order now</span></a></p><p>Green**<br><a href="/order/catalog/product/12369010"><span class="btn btn-primary">Order now</span></a></p></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/12532024"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td colspan="3"><small>*Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.<br> **Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.</small></td></tr></tbody></table></div></div></div><div class="end"></div></div></div><div id="container-bf65cc05-e4a5-49fe-8385-fefb0c8d52b9" class="tab-pane"><div class="container-bf65cc05-e4a5-49fe-8385-fefb0c8d52b9 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Long-range PCR </h3></div><div class="text parbase section"><p>Long-range PCR is a technique used to amplify DNA fragments that are longer than 5 kb, which is larger than the typical limit of standard PCR. It involves modifying the PCR conditions and utilizing specialized DNA polymerases to overcome the challenges associated with amplifying longer DNA sequences. </p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" style="width: 100%;" id="table-648623537"><tbody><tr><th width="33%"> </th><th style="text-align: center;"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-SuperFi-II.jpg" width="40%" title="Platinum SuperFi II DNA Polymerase" alt="Product photo of package and three tubes of reagents. "></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase</a></p></th></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;">Up to 20 kb*</td></tr><tr><td><span class="side"><b>Fidelity vs. <i>Taq</i> enzyme</b></span></td><td style="text-align: center;">>300x</td></tr><tr><td><span class="side"><b>Hot start for enhanced specificity</b></span></td><td style="text-align: center;">Yes</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td></tr><tr><td><b>GC-rich amplification</b></td><td style="text-align: center;">Yes<br></td></tr><tr><td><b>DNA synthesis speed</b></td><td style="text-align: center;">15–30 sec/kb</td></tr><tr><td><span class="side"><b>Stand-alone enzyme</b></span></td><td class="check-cvc" style="text-align: center;">Colorless<br><a href="/order/catalog/product/12361010"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td><span class="side"><b>Master mix format</b></span></td><td style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/12368010"><span class="btn btn-primary">Order now</span></a></p><p>Green**<br><a href="/order/catalog/product/12369010"><span class="btn btn-primary">Order now</span></a></p></td></tr><tr><td colspan="2"><small>*Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.<br> **Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.</small></td></tr></tbody></table></div><p><b>White paper</b>: <span class="icon-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/gibson-assembly-ex-cloning-large-complex-assemblies-white-paper.pdf" target="_blank">Assembly of large PCR amplicons by the Gibson Assembly method</a></p><p> </p></div></div><div class="end"></div></div></div><div id="container-9f90d0a8-fc44-47ed-92d9-9e83f7c3b7b0" class="tab-pane"><div class="container-9f90d0a8-fc44-47ed-92d9-9e83f7c3b7b0 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Multiplex PCR </h3></div><div class="text parbase section"><p>Multiplex PCR is a technique that allows for the concurrent amplification of multiple target DNA sequences within a single PCR reaction. It involves the use of multiple primer sets, each specific to a different target sequence, but amplified in a single reaction mixture.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--423345922"><tbody><tr><th width="25%"> </th><th style="text-align: center;" width="25%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-SuperFi-II.jpg" width="70%" title="Platinum SuperFi II DNA Polymerase" alt="Product package and three tubes of reagents"></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase</a></p></th><th style="text-align: center;" width="25%"><p><a href="/order/catalog/product/4464268"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-multiplex-PCR-MM.jpg" width="70%" title="Platinum Multiplex PCR Master Mix" alt="Product package and two tubes of reagents"></a></p><a href="/order/catalog/product/4464268"></a><p><a href="/order/catalog/product/4464268">Platinum Multiplex PCR Master Mix</a><br></p></th></tr><tr><td><span class="side"><b>No. of amplicons in single reaction </b></span></td><td style="text-align: center;">Up to 15-plex</td><td style="text-align: center;">Up to 20-plex</td></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;">Up to 2.5 kb</td><td style="text-align: center;">Up to 2.5 kb</td></tr><tr><td><span class="side"><b>Hot start for enhanced specificity</b></span></td><td style="text-align: center;">Yes</td><td class="check-cvc" style="text-align: center;">Yes</td></tr><tr><td><b>Fidelity vs. <i>Taq </i>enzyme</b></td><td style="text-align: center;">>300x</td><td style="text-align: center;">1x</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>GC-rich amplification</b></td><td style="text-align: center;">Yes<br></td><td style="text-align: center;">No</td></tr><tr><td><span class="side"><b>Stand-alone enzyme</b></span></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/12361010"><span class="btn btn-primary">Order now</span></a></td><td style="text-align: center;">N/A</td></tr><tr><td><span class="side"><b>Master mix format </b></span></td><td class="check-cvc" style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/12368010"><span class="btn btn-primary">Order now</span></a></p><p>Green*<br><a href="/order/catalog/product/12369010"><span class="btn btn-primary">Order now</span></a></p></td><td class="check-cvc" style="text-align: center;">Colorless<br><a href="/order/catalog/product/4464268"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td colspan="3"><small>*Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.</small></td></tr></tbody></table></div></div><div class="text parbase section"><p><b>Application note</b>: <span class="icon-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/multiplex-pcr-platinum-superfi-app-note.pdf" target="_blank">Multiplex PCR with Platinum SuperFi II DNA</a></p></div></div><div class="end"></div></div></div><div id="container-e7a1f667-1499-47ad-a376-041f895c39b0" class="tab-pane"><div class="container-e7a1f667-1499-47ad-a376-041f895c39b0 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><h3>GC-rich PCR</h3><p>GC-rich PCR is amplification of DNA fragments with high GC content. GC-rich regions of DNA often form stable secondary structures, such as hairpins or G-quadruplexes, that pose a challenge to efficient DNA amplification. Our enzymes are designed to amplify DNA sequences with >65% GC content.</p><div class="table-component-wrapper"><table class="rte-table" id="table--1928350812"><tbody><tr><th width="25%"> </th><th style="text-align: center;" width="33%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-SuperFi-II.jpg" width="70%" title="Platinum SuperFi II DNA Polymerase" alt="Product photo of package and three tubes of reagents."></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase</a><br></p></th><th style="text-align: center;" width="33%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-II-Taq-Hot-Start-Polymerase.jpg" width="70%" title="Platinum II Taq Hot-start DNA Polymerase" alt="product package and three tubes of reagents"></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html">Platinum II <i>Taq</i> DNA Polymerase</a><br></p></th></tr><tr><td><b>Fidelity vs. <i>Taq</i> enzyme</b></td><td style="text-align: center;">>300x</td><td style="text-align: center;">1x</td></tr><tr><td><b>Hot start for enhanced specificity</b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">Yes</td></tr><tr><td><b>Efficient amplification of >65% GC sequences</b></td><td style="text-align: center;">Yes<br></td><td style="text-align: center;">Yes</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">Yes</td></tr><tr><td><b>Speed</b></td><td style="text-align: center;">15–30 sec/kb</td><td style="text-align: center;">15 sec/kb</td></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;">Up to 20 kb</td><td style="text-align: center;">Up to 5 kb</td></tr><tr><td><b>Amplicon overhangs</b></td><td style="text-align: center;">Blunt</td><td style="text-align: center;">3′ A</td></tr><tr><td><b>Stand-alone enzyme</b></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/12361010"><span class="btn btn-primary">Order now</span></a></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/14966001"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td><b>Master mix format</b></td><td style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/12368010"><span class="btn btn-primary">Order now</span></a></p><p>Green*<br><a href="/order/catalog/product/12369010"><span class="btn btn-primary">Order now</span></a></p></td><td style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/14000012"><span class="btn btn-primary">Order now</span></a></p><p>Green*<br><a href="/order/catalog/product/14001012"><span class="btn btn-primary">Order now</span></a></p></td></tr><tr><td colspan="3"><small>*Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.<br></small></td></tr></tbody></table></div></div><div class="text parbase section"><p><b>Application note</b>: <span class="icon-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/qpcr-platinum-direct-app-note.pdf" target="_blank">Detection of target DNA in cell samples by direct PCR</a></p></div></div><div class="end"></div></div></div><div id="container-7cac2fd4-b87f-43fd-88a3-3d461299747d" class="tab-pane"><div class="container-7cac2fd4-b87f-43fd-88a3-3d461299747d tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Direct PCR </h3></div><div class="text parbase section"><p>Direct PCR is a streamlined technique that allows for DNA amplification directly from various biological samples without DNA extraction or purification steps.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" style="width: 100%;" id="table-372925325"><tbody><tr><th width="33%"> </th><th style="text-align: center;"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-Direct-MM.jpg" width="40%"" title="Platinum Direct PCR Master Mix" alt="Product photo of package, four tubes of reagents, and one vial of lysis buffer"></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html">Platinum Direct PCR Universal Master Mix</a></p></th></tr><tr><td><span class="side"><b>Works across samples of various origins</b></span></td><td style="text-align: center;">Yes</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td></tr><tr><td><b>Hot start for enhanced specificity</b></td><td style="text-align: center;">Yes</td></tr><tr><td><span class="side"><b>Fidelity vs. <i>Taq</i> enzyme</b></span></td><td style="text-align: center;">1x</td></tr><tr><td><b>GC-rich amplification</b></td><td style="text-align: center;">Yes</td></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;"> Up to 8 kb*</td></tr><tr><td><b>Speed</b></td><td style="text-align: center;">20 sec/kb</td></tr><tr><td><span class="side"><b>Stand-alone enzyme</b></span></td><td class="check-cvc" style="text-align: center;">N/A</td></tr><tr><td><span class="side"><b>Master mix format</b></span></td><td style="text-align: center;">Green**<br><a href="/order/catalog/product/a44647100"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td colspan="2"><small>*Using the <a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html#s2a3">lysis protocol</a>.<br> **Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.</small></td></tr></tbody></table></div><p><b>Application note</b>: <span class="icon-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/qpcr-platinum-direct-app-note.pdf" target="_blank">Detection of target DNA in cell samples by direct PCR</a></p></div></div><div class="end"></div></div></div></div></div><div class="workflow-accordion accordion" id="accordion-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainer"><div class="accordion-group"><div class="accordion-heading active"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainer" href="#collapse1"><span class="caret"></span><strong> Hot-start PCR </strong></a></div><div id="collapse1" class="accordion-body collapse in "><div class="accordion-inner container-6cb12e16-4623-4d38-97b2-f8822de98181"><div class="container-6cb12e16-4623-4d38-97b2-f8822de98181 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Hot-start PCR </h3></div><div class="text parbase section"><p>Hot-start PCR is a type of PCR where DNA polymerase activity is inhibited until the initial denaturation step. This inhibition is achieved through various methods, such as antibodies, affibodies, or chemical modifications of the polymerase. The benefits of hot-start PCR include increased specificity and yield.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--390942496"><tbody><tr><th width="15%"> </th><th style="text-align: center;" width="25%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-II-Taq-Hot-Start-Polymerase.jpg" width="70%" title="Platinum II Taq Hot-start DNA Polymerase" alt="product package and three tubes of reagents"><br></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html">Platinum II <i>Taq </i>Hot-Start DNA Polymerase</a><br></p></th><th style="text-align: center;" width="25%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/amplitaq-gold-dna-polymerases.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/AmpliTaq-Gold-360.jpg" width="70%" title="AmpliTaq Gold 360 DNA Polymerase" alt="product package and four tubes of reagents"><br></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/amplitaq-gold-dna-polymerases.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/amplitaq-gold-dna-polymerases.html">AmpliTaq Gold 360 DNA Polymerase</a><br></p></th></tr><tr><td><span class="side"><b>Hot-start technology</b></span></td><td style="text-align: center;">Antibody</td><td style="text-align: center;">Chemical</td></tr><tr><td><span class="side"><b>Enzyme activation time</b></span></td><td style="text-align: center;">2 min</td><td style="text-align: center;">10 min</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>DNA synthesis speed</b></td><td style="text-align: center;">15 sec/kb</td><td style="text-align: center;">60 sec/kb</td></tr><tr><td><b>Flexible extension step*</b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>Inhibitor tolerance</b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;">Up to 5 kb</td><td style="text-align: center;">Up to 5 kb</td></tr><tr><td><span class="side"><b>GC-rich format</b></span></td><td class="check-cvc" style="text-align: center;">Yes</td><td class="check-cvc" style="text-align: center;">Yes</td></tr><tr><td><span class="side"><b>Stand-alone enzyme</b></span></td><td style="text-align: center;">Colorless†<br><a href="/order/catalog/product/14966001"><span class="btn btn-primary">Order now</span></a></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/4398813"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td><span class="side"><b>Master mix format</b></span></td><td style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/14000012"><span class="btn btn-primary">Order now</span></a></p><p>Green**<br><a href="/order/catalog/product/14001012"><span class="btn btn-primary">Order now</span></a></p></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/4398881"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td colspan="3"><small>*The extension step can be extended up to 60 sec/kb without the effect of specificity.<br> **Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.<br> †<a href="/order/catalog/product/14966123">Green buffer</a> available as separate item for use with stand-alone enzyme.</small></td></tr></tbody></table></div><div class="alert alert-info"><p><b>Note</b>: The original Platinum <i>Taq</i> DNA Polymerase is available in the formats of <a href="/order/catalog/product/10966018">stand alone</a>, <a href="/order/catalog/product/11966034">stand alone with gel-loading dyes</a>, <a href="/order/catalog/product/13000012">master mix</a>, and <a href="/order/catalog/product/13001012">master mix with gel-loading dyes</a>.<br></p></div></div><div class="well-cmp section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainercontainer-6cb12e16-4623-4d38-97b2-f8822de98181tabwell_a5f2uipartextimage_929c"><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/platinum-dna-polymerases-brochure.pdf" target="_blank"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/tabworkflowcontainer/container-6cb12e16-4623-4d38-97b2-f8822de98181/tab/well_a5f2/uipar/textimage_929c/image.img.full.high.jpg/1730321144922.jpg' media="(min-width: 1px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/tabworkflowcontainer/container-6cb12e16-4623-4d38-97b2-f8822de98181/tab/well_a5f2/uipar/textimage_929c/image.img.full.high.jpg/1730321144922.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/tabworkflowcontainer/container-6cb12e16-4623-4d38-97b2-f8822de98181/tab/well_a5f2/uipar/textimage_929c/image.img.320.high.jpg/1730321144922.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/tabworkflowcontainer/container-6cb12e16-4623-4d38-97b2-f8822de98181/tab/well_a5f2/uipar/textimage_929c/image.img.480.high.jpg/1730321144922.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/tabworkflowcontainer/container-6cb12e16-4623-4d38-97b2-f8822de98181/tab/well_a5f2/uipar/textimage_929c/image.img.320.medium.jpg/1730321144922.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/tabworkflowcontainer/container-6cb12e16-4623-4d38-97b2-f8822de98181/tab/well_a5f2/uipar/textimage_929c/image.img.620.high.jpg/1730321144922.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/tabworkflowcontainer/container-6cb12e16-4623-4d38-97b2-f8822de98181/tab/well_a5f2/uipar/textimage_929c/image.img.320.low.jpg/1730321144922.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/tabworkflowcontainer/container-6cb12e16-4623-4d38-97b2-f8822de98181/tab/well_a5f2/uipar/textimage_929c/image.img.480.medium.jpg/1730321144922.jpg" alt='Platinum DNA polymerases brochure thumbnail' title='A hot start towards your PCR destinations'></picture></div><style> #cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainercontainer-6cb12e16-4623-4d38-97b2-f8822de98181tabwell_a5f2uipartextimage_929c { max-width: 100%; } #cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainercontainer-6cb12e16-4623-4d38-97b2-f8822de98181tabwell_a5f2uipartextimage_929c img { width: 100%; max-width: 100px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><h2>A hot start towards your PCR destinations</h2><p>Find comparison of technical specifications between Platinum II Taq Hot-Start and Platinum Taq DNA Polymerases</p><p><a href="https://assets.thermofisher.com/TFS-Assets/BID/brochures/platinum-dna-polymerases-brochure.pdf" target="_blank" class="btn btn-primary" adhocenable="false">Download the brochure</a></p></div></div><div class="clearfix"></div></div></div></div></div></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainer" href="#collapse2"><span class="caret"></span><strong> High-fidelity PCR </strong></a></div><div id="collapse2" class="accordion-body collapse "><div class="accordion-inner container-a0c70367-ad37-4d5c-9ce2-dd1bd1f0a536"><div class="container-a0c70367-ad37-4d5c-9ce2-dd1bd1f0a536 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> High-fidelity PCR </h3></div><div class="text parbase section"><p>High fidelity PCR, also known as proofreading PCR, is a technique that utilizes DNA polymerases with a built-in proofreading capability to ensure accurate sequence amplification with no errors introduced in the sequence. </p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table--1387186044"><tbody><tr><th width="15%"> </th><th style="text-align: center;" width="33%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-SuperFi-II.jpg" width="70%" title="Platinum SuperFi II DNA Polymerase" alt="Product package and three tubes of reagents"></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><span>Platinum SuperFi II DNA Polymerase</span></a><br></p></th><th style="text-align: center;" width="33%"><p><a href="/order/catalog/product/11304011"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-II-Taq-Hot-Start-DNA-Polymerase.jpg" width="70%" title="Platinum II Taq Hot-start DNA Polymerase" alt="Product package and three tubes of reagents"></a></p><a href="/order/catalog/product/11304011"></a><p><a href="/order/catalog/product/11304011"><span>Platinum <i>Taq</i> DNA Polymerase High Fidelity</span></a><br></p></th></tr><tr><td><span class="side"><b>Fidelity vs. <i>Taq</i> enzyme</b></span></td><td style="text-align: center;">>300x</td><td style="text-align: center;">6x</td></tr><tr><td><span class="side"><b>Hot start for enhanced specificity</b></span></td><td class="check-cvc" style="text-align: center;">Yes</td><td class="check-cvc" style="text-align: center;">Yes</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;">Up to 20 kb*</td><td style="text-align: center;">Up to 20 kb</td></tr><tr><td><b>Amplicon overhangs</b></td><td class="check-cvc" style="text-align: center;">Blunt</td><td style="text-align: center;">3′ A/Blunt<br></td></tr><tr><td><span class="side"><b>GC-rich amplification</b></span></td><td class="check-cvc" style="text-align: center;">Yes<br></td><td style="text-align: center;">No</td></tr><tr><td><span class="side"><b>Stand-alone enzyme</b></span></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/12361010"><span class="btn btn-primary">Order now</span></a></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/11304011"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td><span class="side"><b>Master mix format</b></span></td><td style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/12368010"><span class="btn btn-primary">Order now</span></a></p><p>Green**<br><a href="/order/catalog/product/12369010"><span class="btn btn-primary">Order now</span></a></p></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/12532024"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td colspan="3"><small>*Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.<br> **Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.</small></td></tr></tbody></table></div></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainer" href="#collapse3"><span class="caret"></span><strong> Long-range PCR </strong></a></div><div id="collapse3" class="accordion-body collapse "><div class="accordion-inner container-bf65cc05-e4a5-49fe-8385-fefb0c8d52b9"><div class="container-bf65cc05-e4a5-49fe-8385-fefb0c8d52b9 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Long-range PCR </h3></div><div class="text parbase section"><p>Long-range PCR is a technique used to amplify DNA fragments that are longer than 5 kb, which is larger than the typical limit of standard PCR. It involves modifying the PCR conditions and utilizing specialized DNA polymerases to overcome the challenges associated with amplifying longer DNA sequences. </p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" style="width: 100%;" id="table-296207287"><tbody><tr><th width="33%"> </th><th style="text-align: center;"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-SuperFi-II.jpg" width="40%" title="Platinum SuperFi II DNA Polymerase" alt="Product photo of package and three tubes of reagents. "></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase</a></p></th></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;">Up to 20 kb*</td></tr><tr><td><span class="side"><b>Fidelity vs. <i>Taq</i> enzyme</b></span></td><td style="text-align: center;">>300x</td></tr><tr><td><span class="side"><b>Hot start for enhanced specificity</b></span></td><td style="text-align: center;">Yes</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td></tr><tr><td><b>GC-rich amplification</b></td><td style="text-align: center;">Yes<br></td></tr><tr><td><b>DNA synthesis speed</b></td><td style="text-align: center;">15–30 sec/kb</td></tr><tr><td><span class="side"><b>Stand-alone enzyme</b></span></td><td class="check-cvc" style="text-align: center;">Colorless<br><a href="/order/catalog/product/12361010"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td><span class="side"><b>Master mix format</b></span></td><td style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/12368010"><span class="btn btn-primary">Order now</span></a></p><p>Green**<br><a href="/order/catalog/product/12369010"><span class="btn btn-primary">Order now</span></a></p></td></tr><tr><td colspan="2"><small>*Amplification of >20 kb fragment sizes is possible (up to 40 kb), but may require additional optimization of reaction conditions and primer design.<br> **Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.</small></td></tr></tbody></table></div><p><b>White paper</b>: <span class="icon-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/gibson-assembly-ex-cloning-large-complex-assemblies-white-paper.pdf" target="_blank">Assembly of large PCR amplicons by the Gibson Assembly method</a></p><p> </p></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainer" href="#collapse4"><span class="caret"></span><strong> Multiplex PCR </strong></a></div><div id="collapse4" class="accordion-body collapse "><div class="accordion-inner container-9f90d0a8-fc44-47ed-92d9-9e83f7c3b7b0"><div class="container-9f90d0a8-fc44-47ed-92d9-9e83f7c3b7b0 tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Multiplex PCR </h3></div><div class="text parbase section"><p>Multiplex PCR is a technique that allows for the concurrent amplification of multiple target DNA sequences within a single PCR reaction. It involves the use of multiple primer sets, each specific to a different target sequence, but amplified in a single reaction mixture.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" id="table-829738739"><tbody><tr><th width="25%"> </th><th style="text-align: center;" width="25%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-SuperFi-II.jpg" width="70%" title="Platinum SuperFi II DNA Polymerase" alt="Product package and three tubes of reagents"></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase</a></p></th><th style="text-align: center;" width="25%"><p><a href="/order/catalog/product/4464268"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-multiplex-PCR-MM.jpg" width="70%" title="Platinum Multiplex PCR Master Mix" alt="Product package and two tubes of reagents"></a></p><a href="/order/catalog/product/4464268"></a><p><a href="/order/catalog/product/4464268">Platinum Multiplex PCR Master Mix</a><br></p></th></tr><tr><td><span class="side"><b>No. of amplicons in single reaction </b></span></td><td style="text-align: center;">Up to 15-plex</td><td style="text-align: center;">Up to 20-plex</td></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;">Up to 2.5 kb</td><td style="text-align: center;">Up to 2.5 kb</td></tr><tr><td><span class="side"><b>Hot start for enhanced specificity</b></span></td><td style="text-align: center;">Yes</td><td class="check-cvc" style="text-align: center;">Yes</td></tr><tr><td><b>Fidelity vs. <i>Taq </i>enzyme</b></td><td style="text-align: center;">>300x</td><td style="text-align: center;">1x</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">No</td></tr><tr><td><b>GC-rich amplification</b></td><td style="text-align: center;">Yes<br></td><td style="text-align: center;">No</td></tr><tr><td><span class="side"><b>Stand-alone enzyme</b></span></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/12361010"><span class="btn btn-primary">Order now</span></a></td><td style="text-align: center;">N/A</td></tr><tr><td><span class="side"><b>Master mix format </b></span></td><td class="check-cvc" style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/12368010"><span class="btn btn-primary">Order now</span></a></p><p>Green*<br><a href="/order/catalog/product/12369010"><span class="btn btn-primary">Order now</span></a></p></td><td class="check-cvc" style="text-align: center;">Colorless<br><a href="/order/catalog/product/4464268"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td colspan="3"><small>*Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.</small></td></tr></tbody></table></div></div><div class="text parbase section"><p><b>Application note</b>: <span class="icon-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/multiplex-pcr-platinum-superfi-app-note.pdf" target="_blank">Multiplex PCR with Platinum SuperFi II DNA</a></p></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainer" href="#collapse5"><span class="caret"></span><strong> GC-rich PCR </strong></a></div><div id="collapse5" class="accordion-body collapse "><div class="accordion-inner container-e7a1f667-1499-47ad-a376-041f895c39b0"><div class="container-e7a1f667-1499-47ad-a376-041f895c39b0 tabstep"><div class="start"></div><div class="tab parsys"><div class="text parbase section"><h3>GC-rich PCR</h3><p>GC-rich PCR is amplification of DNA fragments with high GC content. GC-rich regions of DNA often form stable secondary structures, such as hairpins or G-quadruplexes, that pose a challenge to efficient DNA amplification. Our enzymes are designed to amplify DNA sequences with >65% GC content.</p><div class="table-component-wrapper"><table class="rte-table" id="table--460743826"><tbody><tr><th width="25%"> </th><th style="text-align: center;" width="33%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-SuperFi-II.jpg" width="70%" title="Platinum SuperFi II DNA Polymerase" alt="Product photo of package and three tubes of reagents."></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase</a><br></p></th><th style="text-align: center;" width="33%"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-II-Taq-Hot-Start-Polymerase.jpg" width="70%" title="Platinum II Taq Hot-start DNA Polymerase" alt="product package and three tubes of reagents"></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html">Platinum II <i>Taq</i> DNA Polymerase</a><br></p></th></tr><tr><td><b>Fidelity vs. <i>Taq</i> enzyme</b></td><td style="text-align: center;">>300x</td><td style="text-align: center;">1x</td></tr><tr><td><b>Hot start for enhanced specificity</b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">Yes</td></tr><tr><td><b>Efficient amplification of >65% GC sequences</b></td><td style="text-align: center;">Yes<br></td><td style="text-align: center;">Yes</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td><td style="text-align: center;">Yes</td></tr><tr><td><b>Speed</b></td><td style="text-align: center;">15–30 sec/kb</td><td style="text-align: center;">15 sec/kb</td></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;">Up to 20 kb</td><td style="text-align: center;">Up to 5 kb</td></tr><tr><td><b>Amplicon overhangs</b></td><td style="text-align: center;">Blunt</td><td style="text-align: center;">3′ A</td></tr><tr><td><b>Stand-alone enzyme</b></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/12361010"><span class="btn btn-primary">Order now</span></a></td><td style="text-align: center;">Colorless<br><a href="/order/catalog/product/14966001"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td><b>Master mix format</b></td><td style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/12368010"><span class="btn btn-primary">Order now</span></a></p><p>Green*<br><a href="/order/catalog/product/12369010"><span class="btn btn-primary">Order now</span></a></p></td><td style="text-align: center;"><p>Colorless<br><a href="/order/catalog/product/14000012"><span class="btn btn-primary">Order now</span></a></p><p>Green*<br><a href="/order/catalog/product/14001012"><span class="btn btn-primary">Order now</span></a></p></td></tr><tr><td colspan="3"><small>*Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.<br></small></td></tr></tbody></table></div></div><div class="text parbase section"><p><b>Application note</b>: <span class="icon-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/qpcr-platinum-direct-app-note.pdf" target="_blank">Detection of target DNA in cell samples by direct PCR</a></p></div></div><div class="end"></div></div></div></div></div><div class="accordion-group"><div class="accordion-heading"><a class="accordion-toggle" data-toggle="collapse" data-parent="#accordion-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystabworkflowcontainer" href="#collapse6"><span class="caret"></span><strong> Direct PCR </strong></a></div><div id="collapse6" class="accordion-body collapse "><div class="accordion-inner container-7cac2fd4-b87f-43fd-88a3-3d461299747d"><div class="container-7cac2fd4-b87f-43fd-88a3-3d461299747d tabstep"><div class="start"></div><div class="tab parsys"><div class="heading section"><h3> Direct PCR </h3></div><div class="text parbase section"><p>Direct PCR is a streamlined technique that allows for DNA amplification directly from various biological samples without DNA extraction or purification steps.</p></div><div class="text parbase section"><div class="table-component-wrapper"><table class="rte-table" style="width: 100%;" id="table-522392479"><tbody><tr><th width="33%"> </th><th style="text-align: center;"><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html"><img src="/content/dam/LifeTech/global/life-sciences/bid/sample-prep/2024/08/Platinum-Direct-MM.jpg" width="40%"" title="Platinum Direct PCR Master Mix" alt="Product photo of package, four tubes of reagents, and one vial of lysis buffer"></a></p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html"></a><p><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html">Platinum Direct PCR Universal Master Mix</a></p></th></tr><tr><td><span class="side"><b>Works across samples of various origins</b></span></td><td style="text-align: center;">Yes</td></tr><tr><td><b><a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">Universal annealing temperature of 60°C</a></b></td><td style="text-align: center;">Yes</td></tr><tr><td><b>Hot start for enhanced specificity</b></td><td style="text-align: center;">Yes</td></tr><tr><td><span class="side"><b>Fidelity vs. <i>Taq</i> enzyme</b></span></td><td style="text-align: center;">1x</td></tr><tr><td><b>GC-rich amplification</b></td><td style="text-align: center;">Yes</td></tr><tr><td><b>Amplification length</b></td><td style="text-align: center;"> Up to 8 kb*</td></tr><tr><td><b>Speed</b></td><td style="text-align: center;">20 sec/kb</td></tr><tr><td><span class="side"><b>Stand-alone enzyme</b></span></td><td class="check-cvc" style="text-align: center;">N/A</td></tr><tr><td><span class="side"><b>Master mix format</b></span></td><td style="text-align: center;">Green**<br><a href="/order/catalog/product/a44647100"><span class="btn btn-primary">Order now</span></a></td></tr><tr><td colspan="2"><small>*Using the <a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html#s2a3">lysis protocol</a>.<br> **Contains electrophoresis tracking dyes and a density reagent for direct gel loading of PCR products.</small></td></tr></tbody></table></div><p><b>Application note</b>: <span class="icon-pdf"></span> <a class="cta-link" href="https://assets.thermofisher.com/TFS-Assets/BID/Application-Notes/qpcr-platinum-direct-app-note.pdf" target="_blank">Detection of target DNA in cell samples by direct PCR</a></p></div></div><div class="end"></div></div></div></div></div></div></div><div class="heading section"><h2><br><i>Taq</i> DNA polymerases: Native and recombinant enzymes </h2></div><div class="text parbase section"><p>Native DNA polymerases are naturally occurring enzymes found in various organisms such as bacteria and archaea. <i>Taq</i> DNA polymerase is one of the best-known thermostable DNA polymerases used in PCR amplification of DNA targets. The native enzyme is purified from <i>Thermus aquaticus </i>YT1. The native <i>Taq </i>DNA polymerase is often preferred for amplification of bacterial DNA sequences homologous to <i>E. coli</i> sequences. The recombinant enzyme is a cloned version of native <i>Taq</i> polymerases with improved properties and functionalities.</p></div><div class="divider section"><hr /></div><div class="heading section"><div class="h2" id="highlights"> Platinum DNA polymerases for excellent results </div></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div container-id="cq-colctrl-lt3" comp-id="A1"><div class="heading section"><h3><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html">Platinum SuperFi II DNA Polymerase for high fidelity PCR</a></h3></div></div><div container-id="cq-colctrl-lt3" comp-id="A2"><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystextimage_3f29"><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-high-fidelity-pcr-enzyme.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_3f29/image.img.320.medium.jpg/1730321145361.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_3f29/image.img.480.high.jpg/1730321145361.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_3f29/image.img.620.high.jpg/1730321145361.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_3f29/image.img.full.high.jpg/1730321145361.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_3f29/image.img.320.high.jpg/1730321145361.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_3f29/image.img.480.high.jpg/1730321145361.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_3f29/image.img.320.medium.jpg/1730321145361.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_3f29/image.img.620.high.jpg/1730321145361.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_3f29/image.img.320.low.jpg/1730321145361.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_3f29/image.img.480.medium.jpg/1730321145361.jpg" alt='Product photo of package and three tubes of reagents' title='Platinum SuperFi II DNA Polymerase'></picture></div><style> #cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystextimage_3f29 { max-width: 100%; } #cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystextimage_3f29 img { width: 100%; max-width: 651px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><ul><li><b>Less downstream troubleshooting</b>—ultralow sequence errors due to >300x fidelity of <i>Taq</i> enzyme</li><li><b>Best suited for:</b> cloning, sequencing, mutagenesis</li></ul></div></div><div class="clearfix"></div></div></div><div container-id="cq-colctrl-lt3" comp-id="A3"><div class="text parbase section"><p><a href="/order/catalog/product/12361010"><span class="btn btn-primary">Order now</span></a></p></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="heading section"><h3><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html">Platinum II <i>Taq</i> Hot-Start DNA Polymerase for hot-start PCR</a></h3></div></div><div container-id="cq-colctrl-lt3" comp-id="B2"><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystextimage_f1b8"><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-hot-start-pcr-enzyme.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_f1b8/image.img.320.medium.jpg/1730321145429.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_f1b8/image.img.480.high.jpg/1730321145429.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_f1b8/image.img.620.high.jpg/1730321145429.jpg' media="(min-width: 630px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_f1b8/image.img.full.high.jpg/1730321145429.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_f1b8/image.img.320.high.jpg/1730321145429.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_f1b8/image.img.480.high.jpg/1730321145429.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_f1b8/image.img.320.medium.jpg/1730321145429.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_f1b8/image.img.620.high.jpg/1730321145429.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_f1b8/image.img.320.low.jpg/1730321145429.jpg,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_f1b8/image.img.480.medium.jpg/1730321145429.jpg" alt='Photo of product package and three tubes of reagents' title='Platinum II Taq Hot-start DNA Polymerase'></picture></div><style> #cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystextimage_f1b8 { max-width: 100%; } #cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystextimage_f1b8 img { width: 100%; max-width: 651px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><ul><li><b>Short PCR cycling time</b>—DNA synthesis at 15 sec/kb</li><li><b>Best suited for:</b> gene detection, genotyping, low copy number amplification</li></ul></div></div><div class="clearfix"></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B3"><div class="text parbase section"><p><a href="/order/catalog/product/14966001"><span class="btn btn-primary">Order now</span></a></p></div></div></div><div class="parsys_column cq-colctrl-lt3-c2"><div container-id="cq-colctrl-lt3" comp-id="C1"><div class="heading section"><h3><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html">Platinum Direct PCR Universal Master Mix for direct PCR</a></h3></div></div><div container-id="cq-colctrl-lt3" comp-id="C2"><div class="textimage parbase section"><div class="image" id="cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystextimage_afc1"><a href="/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/platinum-direct-pcr-universal-master-mix.html"><div class="image-container"><picture><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_afc1/image.img.320.high.png/1730321145491.png' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_afc1/image.img.480.high.png/1730321145491.png' media="(min-width: 490px)"><img srcset="https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_afc1/image.img.full.high.png/1730321145491.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_afc1/image.img.320.high.png/1730321145491.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_afc1/image.img.480.high.png/1730321145491.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_afc1/image.img.320.medium.png/1730321145491.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_afc1/image.img.620.high.png/1730321145491.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_afc1/image.img.320.low.png/1730321145491.png,https://www.thermofisher.com/sg/en/home/life-science/pcr/pcr-enzymes-master-mixes/jcr:content/MainParsys/textimage_afc1/image.img.480.medium.png/1730321145491.png" alt='Product photo of package, four tubes of reagents, and one vial of lysis buffer' title='Platinum Direct PCR Master Mix'></picture></div><style> #cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystextimage_afc1 { max-width: 100%; } #cq-textimage-jsp-92d174f3-115d-4166-b9dc-a308f203c2efMainParsystextimage_afc1 img { width: 100%; max-width: 600px; } </style><script type="text/javascript"> $( document ).ready(function() { if(!!window.picturefill){ window.picturefill(); } }); </script></a></div><div class="seo-event-text" data-seo-schema='' data-seo-element='description'><div class="text"><ul><li><b>Simplified workflow</b>—no DNA purification prior to PCR</li><li><b>Best suited for:</b> genotyping, colony screening</li></ul></div></div><div class="clearfix"></div></div></div><div container-id="cq-colctrl-lt3" comp-id="C3"><div class="text parbase section"><p><a href="/order/catalog/product/A44647100"><span class="btn btn-primary">Order now</span></a></p></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p>The new generation of Platinum DNA polymerases come with novel buffers, enabling <a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html">primer annealing at a universal temperature of 60°C</a>. A combination of the innovative buffers, high-performing enzymes, and reliable hot-start technology offers exceptional PCR results even for challenging applications.<a href="/sg/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/pcr-annealing-optimization-universal-annealing.html"></a></p></div><div class="heading section"><h2 id="benefits"><br>Benefits of platinum PCR enzymes </h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><ul><li><b>Enhanced PCR specificity—</b>Antibody-mediated hot-start enzymes with Platinum technology</li><li><b>Circumvent multiple PCR runs</b>—Ability to co-cycle different PCR assays</li><li><b>Detecting multiple targets from one template</b>—Ability to multiplex up to 15 targets</li><li><b>Fewer steps for pipetting and reagent setup</b>—Necessary reaction components and direct gel-loading dyes included in the green master mixes</li></ul></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><ul><li><b>Simplify PCR optimization steps</b>—Protocol with a universal annealing temperature</li><li><b>Robust PCR with difficult targets</b>—High tolerance to inhibitor-containing samples and efficient amplification of GC-rich sequences</li><li><b>Enabling automation or robotic setup</b>—Assembled reactions stable on the benchtop for 8–24 hours</li></ul></div></div></div></div><div style="clear:both"></div><div class="reference parbase section"><div class="cq-dd-paragraph"><div class="well-cmp"><div class="well well-white well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt3"><div class="parsys_column cq-colctrl-lt3-c0"><div 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