CINXE.COM

<!DOCTYPE html> <html class="client-nojs" lang="en" dir="ltr"> <head> <meta charset="UTF-8"/> <title>Acts upstream of or within, negative effect - GO Wiki</title> <script>document.documentElement.className="client-js";RLCONF={"wgBreakFrames":false,"wgSeparatorTransformTable":["",""],"wgDigitTransformTable":["",""],"wgDefaultDateFormat":"dmy","wgMonthNames":["","January","February","March","April","May","June","July","August","September","October","November","December"],"wgRequestId":"Z0gTk_z7pIVC0Vva38I8VwAAEaE","wgCSPNonce":false,"wgCanonicalNamespace":"","wgCanonicalSpecialPageName":false,"wgNamespaceNumber":0,"wgPageName":"Acts_upstream_of_or_within,_negative_effect","wgTitle":"Acts upstream of or within, negative effect","wgCurRevisionId":84281,"wgRevisionId":84281,"wgArticleId":8603,"wgIsArticle":true,"wgIsRedirect":false,"wgAction":"view","wgUserName":null,"wgUserGroups":["*"],"wgCategories":["Gene Product to Term Relations"],"wgPageContentLanguage":"en","wgPageContentModel":"wikitext","wgRelevantPageName":"Acts_upstream_of_or_within,_negative_effect","wgRelevantArticleId":8603,"wgIsProbablyEditable":false,"wgRelevantPageIsProbablyEditable":false, "wgRestrictionEdit":[],"wgRestrictionMove":[],"wgVector2022PreviewPages":[]};RLSTATE={"site.styles":"ready","user.styles":"ready","user":"ready","user.options":"loading","skins.vector.styles.legacy":"ready"};RLPAGEMODULES=["site","mediawiki.page.ready","mediawiki.toc","skins.vector.legacy.js"];</script> <script>(RLQ=window.RLQ||[]).push(function(){mw.loader.implement("user.options@12s5i",function($,jQuery,require,module){mw.user.tokens.set({"patrolToken":"+\\","watchToken":"+\\","csrfToken":"+\\"});});});</script> <link rel="stylesheet" href="/load.php?lang=en&modules=skins.vector.styles.legacy&only=styles&skin=vector"/> <script async="" src="/load.php?lang=en&modules=startup&only=scripts&raw=1&skin=vector"></script> <meta name="ResourceLoaderDynamicStyles" content=""/> <link rel="stylesheet" href="/load.php?lang=en&modules=site.styles&only=styles&skin=vector"/> <meta name="generator" content="MediaWiki 1.40.0"/> <meta name="robots" content="max-image-preview:standard"/> <meta name="format-detection" content="telephone=no"/> <meta name="viewport" content="width=1000"/> <link rel="icon" href="/favicon.ico"/> <link rel="search" type="application/opensearchdescription+xml" href="/opensearch_desc.php" title="GO Wiki (en)"/> <link rel="EditURI" type="application/rsd+xml" href="https://wiki.geneontology.org/api.php?action=rsd"/> <link rel="alternate" type="application/atom+xml" title="GO Wiki Atom feed" href="/index.php?title=Special:RecentChanges&feed=atom"/> </head> <body class="skin-vector-legacy mediawiki ltr sitedir-ltr mw-hide-empty-elt ns-0 ns-subject page-Acts_upstream_of_or_within_negative_effect rootpage-Acts_upstream_of_or_within_negative_effect skin-vector action-view"><div id="mw-page-base" class="noprint"></div> <div id="mw-head-base" class="noprint"></div> <div id="content" class="mw-body" role="main"> <a id="top"></a> <div id="siteNotice"></div> <div class="mw-indicators"> </div> <h1 id="firstHeading" class="firstHeading mw-first-heading"><span class="mw-page-title-main">Acts upstream of or within, negative effect</span></h1> <div id="bodyContent" class="vector-body"> <div id="siteSub" class="noprint">From GO Wiki</div> <div id="contentSub"><div id="mw-content-subtitle"></div></div> <div id="contentSub2"></div> <div id="jump-to-nav"></div> <a class="mw-jump-link" href="#mw-head">Jump to navigation</a> <a class="mw-jump-link" href="#searchInput">Jump to search</a> <div id="mw-content-text" class="mw-body-content mw-content-ltr" lang="en" dir="ltr"><div class="mw-parser-output"><div id="toc" class="toc" role="navigation" aria-labelledby="mw-toc-heading"><input type="checkbox" role="button" id="toctogglecheckbox" class="toctogglecheckbox" style="display:none" /><div class="toctitle" lang="en" dir="ltr"><h2 id="mw-toc-heading">Contents</h2><span class="toctogglespan"><label class="toctogglelabel" for="toctogglecheckbox"></label></span></div> <ul> <li class="toclevel-1 tocsection-1"><a href="#Overview"><span class="tocnumber">1</span> <span class="toctext">Overview</span></a></li> <li class="toclevel-1 tocsection-2"><a href="#Examples_of_Usage"><span class="tocnumber">2</span> <span class="toctext">Examples of Usage</span></a></li> <li class="toclevel-1 tocsection-3"><a href="#Quality_Control_Checks"><span class="tocnumber">3</span> <span class="toctext">Quality Control Checks</span></a></li> <li class="toclevel-1 tocsection-4"><a href="#Relations_Ontology"><span class="tocnumber">4</span> <span class="toctext">Relations Ontology</span></a></li> <li class="toclevel-1 tocsection-5"><a href="#Review_Status"><span class="tocnumber">5</span> <span class="toctext">Review Status</span></a></li> </ul> </div> <h2><span class="mw-headline" id="Overview">Overview</span></h2> <p>The <i>acts upstream of or within, negative effect</i> annotation relation is used when the mechanism relating the gene product's activity to the Biological Process is not known. However, the observed experimental outcome indicates that, ultimately, the gene product's activity has a negative effect on the process. Annotations using this relation often come from mutant phenotypes for which further characterization has not been performed, but the authors wish to report the effect that the gene has on a process. </p> <h2><span class="mw-headline" id="Examples_of_Usage">Examples of Usage</span></h2> <ul><li><a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/?term=26265472">The zebrafish miR-462/miR-731 cluster is induced under hypoxic stress via hypoxia-inducible factor 1伪 and functions in cellular adaptations.</a> <ul><li>Overexpression of miR-462 and miR-731 represses cell proliferation through blocking cell cycle progress of DNA replication, and induces apoptosis.</li> <li>Summary of the paper:</li></ul></li></ul> <p>INTRO </p> <ul><li>The transcription factor HIF1-alpha is hydroxylated at proline residues under normal oxygen conditions ("normoxia"), which targets it for proteosomal degradation. Hence under normal conditions its half life is very short.</li> <li>Under limiting O2 conditions, the prolyl hydroxylase activity is reduced, and HIF1-alpha is stabilized, and interacts with HIF1-beta. <ul><li>The HIF1-alpha/beta dimer activates hundreds of genes involved in hypoxia adaptation via their DNA binding transcription activator activity, binding to HRE (hypoxia-responsive elements)</li></ul></li> <li>Hundreds of genes are also down-regulated in a HIF1-dependent manner, but without direct HIF1-promoter binding</li></ul> <p>RESULTS </p> <ul><li>Deep sequencing of small RNA libraries identified 129 and and 212 novel miRNAs under normoxia and hypoxia conditions, 102 of which in common to both sets (NOTE: these miRNAs were not further studied).</li> <li>To identify known miRNA, the authors looked into the miRBase, that contained 247 known miRNAs. Seven differentially regulated miRNAs were identified: 5 upregulated and 2 downregulated (Table 3).</li> <li>dre-miR-462 and dre-miR-731 are both on a cluster on Chr8 and were shown to be co-upregulated in response to hypoxia (Fig 1) and upon overexpression of HIF1 (Fig 2A), and decreased by knockdown of HIF1</li> <li>A HIF1 binding site was identified 22 nt upstream from the Chr 8 cluster</li> <li><b>Targets</b>: 2050 putative targets of the 8 miRNAs that belong to the same Chr 8 cluster were predicted - involved in multiple pathways and processes. Two of these targets were selected: ddx5 (miR-462 target) and ppmda (miR-731 target)</li> <li>Luciferase assay with the 3'UTR sequence of the ddx5 and ppmda mRNAs showed that these 3'UTR are sufficient to decrease expression of luciferase (Fig 3)</li> <li>ddx5 and mRNA and protein were downregulated by miR-462 mimics and upregulated by miR-462 inhibitors, indicating that the miRNA does contribute to the regulation of expression level of ddx5</li> <li>Assayed effect of miR-462 and miR-731 on proliferation and apoptosis. <ul><li>Both miRNAs significantly repressed proliferation (Fig 4A), blocking cell cycle progression at S phase (Fig 4B,C)</li> <li>Overexpression of miR-462 and miR-731 increased apoptosis (Fig 4E) - in a caspase-3 independent manner</li></ul></li></ul> <p>ANNOTATIONS </p> <ul><li>miR-462 'acts upstream of or within, negative effect' cell proliferation <ul><li>See <a rel="nofollow" class="external free" href="http://noctua.berkeleybop.org/editor/graph/gomodel:5a7e68a100001507">http://noctua.berkeleybop.org/editor/graph/gomodel:5a7e68a100001507</a></li></ul></li></ul> <h2><span class="mw-headline" id="Quality_Control_Checks">Quality Control Checks</span></h2> <h2><span class="mw-headline" id="Relations_Ontology">Relations Ontology</span></h2> <p><a rel="nofollow" class="external text" href="http://purl.obolibrary.org/obo/RO_0004033">acts upstream of or within, negative effect</a> </p> <h2><span class="mw-headline" id="Review_Status">Review Status</span></h2> <p>Last reviewed: March 9, 2018 </p><p><a rel="nofollow" class="external text" href="http://wiki.geneontology.org/index.php/Annotation_Relations">Back to: Annotation Relations</a> </p>    </div> <div class="printfooter" data-nosnippet="">Retrieved from "<a dir="ltr" href="https://wiki.geneontology.org/index.php?title=Acts_upstream_of_or_within,_negative_effect&oldid=84281">https://wiki.geneontology.org/index.php?title=Acts_upstream_of_or_within,_negative_effect&oldid=84281</a>"</div></div> <div id="catlinks" class="catlinks" data-mw="interface"><div id="mw-normal-catlinks" class="mw-normal-catlinks"><a href="/Special:Categories" title="Special:Categories">Category</a>: <ul><li><a href="/Category:Gene_Product_to_Term_Relations" title="Category:Gene Product to Term Relations">Gene Product to Term Relations</a></li></ul></div></div> </div> </div> <div id="mw-navigation"> <h2>Navigation menu</h2> <div id="mw-head"> <nav id="p-personal" class="vector-menu mw-portlet mw-portlet-personal vector-user-menu-legacy" aria-labelledby="p-personal-label" role="navigation" > <h3 id="p-personal-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">Personal tools</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"><li id="pt-login" class="mw-list-item"><a href="/index.php?title=Special:UserLogin&returnto=Acts+upstream+of+or+within%2C+negative+effect" title="You are encouraged to log in; however, it is not mandatory [o]" accesskey="o"><span>Log in</span></a></li></ul> </div> </nav> <div id="left-navigation"> <nav id="p-namespaces" class="vector-menu mw-portlet mw-portlet-namespaces vector-menu-tabs vector-menu-tabs-legacy" aria-labelledby="p-namespaces-label" role="navigation" > <h3 id="p-namespaces-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">Namespaces</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"><li id="ca-nstab-main" class="selected mw-list-item"><a href="/Acts_upstream_of_or_within,_negative_effect" title="View the content page [c]" accesskey="c"><span>Page</span></a></li><li id="ca-talk" class="new mw-list-item"><a href="/index.php?title=Talk:Acts_upstream_of_or_within,_negative_effect&action=edit&redlink=1" rel="discussion" title="Discussion about the content page (page does not exist) [t]" accesskey="t"><span>Discussion</span></a></li></ul> </div> </nav> <nav id="p-variants" class="vector-menu mw-portlet mw-portlet-variants emptyPortlet vector-menu-dropdown" aria-labelledby="p-variants-label" role="navigation" > <input type="checkbox" id="p-variants-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-p-variants" class="vector-menu-checkbox" aria-labelledby="p-variants-label" /> <label id="p-variants-label" aria-label="Change language variant" class="vector-menu-heading " > <span class="vector-menu-heading-label">English</span> </label> <div class="vector-menu-content"> <ul class="vector-menu-content-list"></ul> </div> </nav> </div> <div id="right-navigation"> <nav id="p-views" class="vector-menu mw-portlet mw-portlet-views vector-menu-tabs vector-menu-tabs-legacy" aria-labelledby="p-views-label" role="navigation" > <h3 id="p-views-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">Views</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"><li id="ca-view" class="selected mw-list-item"><a href="/Acts_upstream_of_or_within,_negative_effect"><span>Read</span></a></li><li id="ca-viewsource" class="mw-list-item"><a href="/index.php?title=Acts_upstream_of_or_within,_negative_effect&action=edit" title="This page is protected.
You can view its source [e]" accesskey="e"><span>View source</span></a></li><li id="ca-history" class="mw-list-item"><a href="/index.php?title=Acts_upstream_of_or_within,_negative_effect&action=history" title="Past revisions of this page [h]" accesskey="h"><span>View history</span></a></li></ul> </div> </nav> <nav id="p-cactions" class="vector-menu mw-portlet mw-portlet-cactions emptyPortlet vector-menu-dropdown" aria-labelledby="p-cactions-label" role="navigation" title="More options" > <input type="checkbox" id="p-cactions-checkbox" role="button" aria-haspopup="true" data-event-name="ui.dropdown-p-cactions" class="vector-menu-checkbox" aria-labelledby="p-cactions-label" /> <label id="p-cactions-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">More</span> </label> <div class="vector-menu-content"> <ul class="vector-menu-content-list"></ul> </div> </nav> <div id="p-search" role="search" class="vector-search-box-vue vector-search-box-show-thumbnail vector-search-box-auto-expand-width vector-search-box"> <div> <form action="/index.php" id="searchform" class="vector-search-box-form"> <div id="simpleSearch" class="vector-search-box-inner" data-search-loc="header-navigation"> <input class="vector-search-box-input" type="search" name="search" placeholder="Search GO Wiki" aria-label="Search GO Wiki" autocapitalize="sentences" title="Search GO Wiki [f]" accesskey="f" id="searchInput" > <input type="hidden" name="title" value="Special:Search"> <input id="mw-searchButton" class="searchButton mw-fallbackSearchButton" type="submit" name="fulltext" title="Search the pages for this text" value="Search"> <input id="searchButton" class="searchButton" type="submit" name="go" title="Go to a page with this exact name if it exists" value="Go"> </div> </form> </div> </div> </div> </div> <div id="mw-panel" class="vector-legacy-sidebar"> <div id="p-logo" role="banner"> <a class="mw-wiki-logo" href="/Main_Page" title="Visit the main page"></a> </div> <nav id="p-navigation" class="vector-menu mw-portlet mw-portlet-navigation vector-menu-portal portal" aria-labelledby="p-navigation-label" role="navigation" > <h3 id="p-navigation-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">Navigation</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"><li id="n-Wiki-Main-Page" class="mw-list-item"><a href="/Main_Page"><span>Wiki Main Page</span></a></li></ul> </div> </nav> <nav id="p-GOC_Resources" class="vector-menu mw-portlet mw-portlet-GOC_Resources vector-menu-portal portal" aria-labelledby="p-GOC_Resources-label" role="navigation" > <h3 id="p-GOC_Resources-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">GOC Resources</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"><li id="n-Project-organization" class="mw-list-item"><a href="/Project_organization"><span>Project organization</span></a></li><li id="n-New-member-onboarding" class="mw-list-item"><a href="/New_member_onboarding"><span>New member onboarding</span></a></li><li id="n-Meetings" class="mw-list-item"><a href="/Consortium_Meetings_and_Workshops"><span>Meetings</span></a></li><li id="n-Data-release-pipeline" class="mw-list-item"><a href="/Release_Pipeline"><span>Data release pipeline</span></a></li><li id="n-Publications,-talks-and-posters" class="mw-list-item"><a href="/Publications,_tutorials,_talks,_posters"><span>Publications, talks and posters</span></a></li><li id="n-Internal-email-lists---Subscribe-and-unsubscribe" class="mw-list-item"><a href="/Internal_email_lists"><span>Internal email lists - Subscribe and unsubscribe</span></a></li><li id="n-Outreach" class="mw-list-item"><a href="/Outreach"><span>Outreach</span></a></li><li id="n-Member-offboarding" class="mw-list-item"><a href="/Member_Offboarding"><span>Member offboarding</span></a></li></ul> </div> </nav> <nav id="p-Annotation" class="vector-menu mw-portlet mw-portlet-Annotation vector-menu-portal portal" aria-labelledby="p-Annotation-label" role="navigation" > <h3 id="p-Annotation-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">Annotation</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"><li id="n-Annotation-Group" class="mw-list-item"><a href="/Annotation"><span>Annotation Group</span></a></li><li id="n-Annotation-Guidelines" class="mw-list-item"><a href="/Annotation_guidelines"><span>Annotation Guidelines</span></a></li><li id="n-Phylogenetic-Annotation" class="mw-list-item"><a href="/Phylogenetic_Annotation_Project"><span>Phylogenetic Annotation</span></a></li><li id="n-Quality-Control" class="mw-list-item"><a href="/Quality_Control"><span>Quality Control</span></a></li></ul> </div> </nav> <nav id="p-Ontology" class="vector-menu mw-portlet mw-portlet-Ontology vector-menu-portal portal" aria-labelledby="p-Ontology-label" role="navigation" > <h3 id="p-Ontology-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">Ontology</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"><li id="n-Ontology-Group" class="mw-list-item"><a href="/Ontology_Development"><span>Ontology Group</span></a></li><li id="n-Ontology-editors'-manual" class="mw-list-item"><a href="/Ontology_editors%27_manual"><span>Ontology editors' manual</span></a></li><li id="n-Ontology-weekly-calls" class="mw-list-item"><a href="/Ontology_Development#Agenda_and_Minutes"><span>Ontology weekly calls</span></a></li></ul> </div> </nav> <nav id="p-Software_and_Utilities|Software_and_Utilities" class="vector-menu mw-portlet mw-portlet-Software_and_Utilities_Software_and_Utilities emptyPortlet vector-menu-portal portal" aria-labelledby="p-Software_and_Utilities|Software_and_Utilities-label" role="navigation" > <h3 id="p-Software_and_Utilities|Software_and_Utilities-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">Software and Utilities|Software and Utilities</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"></ul> </div> </nav> <nav id="p-Management" class="vector-menu mw-portlet mw-portlet-Management vector-menu-portal portal" aria-labelledby="p-Management-label" role="navigation" > <h3 id="p-Management-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">Management</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"><li id="n-Project-management" class="mw-list-item"><a href="/Project_organization#GO_Managers"><span>Project management</span></a></li><li id="n-Project-stand-up-meetings" class="mw-list-item"><a href="/Project_stand_up_meetings"><span>Project stand up meetings</span></a></li><li id="n-Progress-Reports" class="mw-list-item"><a href="/Progress_Reports"><span>Progress Reports</span></a></li></ul> </div> </nav> <nav id="p-Best_Practices" class="vector-menu mw-portlet mw-portlet-Best_Practices vector-menu-portal portal" aria-labelledby="p-Best_Practices-label" role="navigation" > <h3 id="p-Best_Practices-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">Best Practices</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"><li id="n-How-to-edit-this-wiki" class="mw-list-item"><a href="/Wiki_best_practice"><span>How to edit this wiki</span></a></li><li id="n-Authorship-guidelines" class="mw-list-item"><a href="/Authorship_guidelines"><span>Authorship guidelines</span></a></li><li id="n-Reviewing-manuscripts-using-GO" class="mw-list-item"><a href="/Reviewing_papers"><span>Reviewing manuscripts using GO</span></a></li><li id="n-Identifiers-format" class="mw-list-item"><a href="/Identifiers"><span>Identifiers format</span></a></li></ul> </div> </nav> <nav id="p-tb" class="vector-menu mw-portlet mw-portlet-tb vector-menu-portal portal" aria-labelledby="p-tb-label" role="navigation" > <h3 id="p-tb-label" class="vector-menu-heading " > <span class="vector-menu-heading-label">Tools</span> </h3> <div class="vector-menu-content"> <ul class="vector-menu-content-list"><li id="t-whatlinkshere" class="mw-list-item"><a href="/Special:WhatLinksHere/Acts_upstream_of_or_within,_negative_effect" title="A list of all wiki pages that link here [j]" accesskey="j"><span>What links here</span></a></li><li id="t-recentchangeslinked" class="mw-list-item"><a href="/Special:RecentChangesLinked/Acts_upstream_of_or_within,_negative_effect" rel="nofollow" title="Recent changes in pages linked from this page [k]" accesskey="k"><span>Related changes</span></a></li><li id="t-specialpages" class="mw-list-item"><a href="/Special:SpecialPages" title="A list of all special pages [q]" accesskey="q"><span>Special pages</span></a></li><li id="t-print" class="mw-list-item"><a href="javascript:print();" rel="alternate" title="Printable version of this page [p]" accesskey="p"><span>Printable version</span></a></li><li id="t-permalink" class="mw-list-item"><a href="/index.php?title=Acts_upstream_of_or_within,_negative_effect&oldid=84281" title="Permanent link to this revision of this page"><span>Permanent link</span></a></li><li id="t-info" class="mw-list-item"><a href="/index.php?title=Acts_upstream_of_or_within,_negative_effect&action=info" title="More information about this page"><span>Page information</span></a></li></ul> </div> </nav> </div> </div> <footer id="footer" class="mw-footer" role="contentinfo" > <ul id="footer-info"> <li id="footer-info-lastmod"> This page was last edited on 9 February 2023, at 05:42.</li> </ul> <ul id="footer-places"> <li id="footer-places-privacy"><a href="/GO_Wiki:Privacy_policy">Privacy policy</a></li> <li id="footer-places-about"><a href="/GO_Wiki:About">About GO Wiki</a></li> <li id="footer-places-disclaimers"><a href="/GO_Wiki:General_disclaimer">Disclaimers</a></li> </ul> <ul id="footer-icons" class="noprint"> <li id="footer-poweredbyico"><a href="https://www.mediawiki.org/"><img src="/resources/assets/poweredby_mediawiki_88x31.png" alt="Powered by MediaWiki" srcset="/resources/assets/poweredby_mediawiki_132x47.png 1.5x, /resources/assets/poweredby_mediawiki_176x62.png 2x" width="88" height="31" loading="lazy"/></a></li> </ul> </footer> <script src="https://www.googletagmanager.com/gtag/js?id=G-C69T3LCEZ3" async=""></script><script> window.dataLayer = window.dataLayer || []; function gtag(){dataLayer.push(arguments);} gtag('js', new Date()); gtag('config', 'G-C69T3LCEZ3', {}); </script> <script>(RLQ=window.RLQ||[]).push(function(){mw.config.set({"wgBackendResponseTime":209,"wgPageParseReport":{"limitreport":{"cputime":"0.006","walltime":"0.006","ppvisitednodes":{"value":14,"limit":1000000},"postexpandincludesize":{"value":0,"limit":2097152},"templateargumentsize":{"value":0,"limit":2097152},"expansiondepth":{"value":2,"limit":100},"expensivefunctioncount":{"value":0,"limit":100},"unstrip-depth":{"value":0,"limit":20},"unstrip-size":{"value":0,"limit":5000000},"timingprofile":["100.00% 0.000 1 -total"]},"cachereport":{"timestamp":"20241128065411","ttl":86400,"transientcontent":false}}});});</script> </body> </html>