Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus

<!doctype html> <html lang="en"> <head> <meta charset="UTF-8" /> <title>Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus</title> <meta name="keywords" content="archaeal genetics, CRISPR‐Cas, CRISPR escape mutations, dual plasmid vectors, <i>Saccharolobus–E. coli</i> shuttle vectors" /> <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1.0, user-scalable=0" /> <meta http-equiv="X-UA-Compatible" content="IE=edge,Chrome=1" /> <meta name="robots" content="noarchive" /> <meta property="og:title" content="Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for <i>Saccharolobus islandicus</i>" /> <meta property="og:type" content="Article" /> <meta property="og:url" content="https://www.sciopen.com/article/10.1002/mlf2.12107" /> <meta property="og:site_name" content="SciOpen" /> <meta property="og:description" content="<p><i>Saccharolobus islandicus</i> REY15A represents one of the very few archaeal models with versatile genetic tools, which include efficient genome editing, gene silencing, and robust protein expression systems. However, plasmid vectors constructed for this crenarchaeon thus far are based solely on the pRN2 cryptic plasmid. Although this plasmid coexists with pRN1 in its original host, early attempts to test pRN1‐based vectors consistently failed to yield any stable host–vector system for <i>Sa. islandicus</i>. We hypothesized that this failure could be due to the occurrence of CRISPR immunity against pRN1 in this archaeon. We identified a putative target sequence in <i>orf904</i> encoding a putative replicase on pRN1 (target N1). Mutated targets (N1a, N1b, and N1c) were then designed and tested for their capability to escape the host CRISPR immunity by using a plasmid interference assay. The results revealed that the original target triggered CRISPR immunity in this archaeon, whereas all three mutated targets did not, indicating that all the designed target mutations evaded host immunity. These mutated targets were then incorporated into <i>orf904</i> individually, yielding corresponding mutated pRN1 backbones with which shuttle plasmids were constructed (pN1aSD, pN1bSD, and pN1cSD). <i>Sa. islandicus</i> transformation revealed that pN1aSD and pN1bSD were functional shuttle vectors, but pN1cSD lost the capability for replication. These results indicate that the missense mutations in the conserved helicase domain in pN1c inactivated the replicase. We further showed that pRN1‐based and pRN2‐based vectors were stably maintained in the archaeal cells either alone or in combination, and this yielded a dual plasmid system for genetic study with this important archaeal model.</p>" /> <meta name="citation_doi" content="10.1002/mlf2.12107" /> <meta name="dc.identifier" content="10.1002/mlf2.12107" /> <meta name="citation_journal_title" content="mLife" /> <meta name="citation_title" content="Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for <i>Saccharolobus islandicus</i>" /> <meta content="archaeal genetics" name="citation_keywords" /><meta content="CRISPR‐Cas" name="citation_keywords" /><meta content="CRISPR escape mutations" name="citation_keywords" /><meta content="dual plasmid vectors" name="citation_keywords" /><meta content="<i>Saccharolobus–E. coli</i> shuttle vectors" name="citation_keywords" /> <meta name="citation_volume" content="3" /> <meta name="citation_issue" content="1" /> <meta name="citation_firstpage" content="119" /> <meta name="citation_lastpage" content="128" /> <meta name="citation_publication_date" content="2024/3/1" /> <meta name="citation_online_date" content="2024/3/20" /> <meta name="citation_issn" content="2097-1699" /> <meta name="citation_language" content="en" /> <meta name="citation_author" content="Pengpeng Zhao" /> <meta name="citation_author_institution" content=" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. " /> <meta name="citation_author" content="Xiaonan Bi" /> <meta name="citation_author_institution" content=" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. " /> <meta name="citation_author" content="Xiaoning Wang" /> <meta name="citation_author_institution" content=" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. " /> <meta name="citation_author" content="Xu Feng" /> <meta name="citation_author_institution" content=" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. " /> <meta name="citation_author" content="Yulong Shen" /> <meta name="citation_author_institution" content=" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. " /> <meta name="citation_author" content="Guanhua Yuan" /> <meta name="citation_author_institution" content=" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. " /> <meta name="citation_author" content="Qunxin She" /> <meta name="citation_author_institution" content=" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. " /> <meta name="citation_abstract_html_url" content="https://www.sciopen.com/article/10.1002/mlf2.12107" /> <meta name="citation_fulltext_html_url" content="https://www.sciopen.com/article/10.1002/mlf2.12107" /> <meta name="citation_date" content="2024/03/01" /> <meta name="citation_pdf_url" content="https://sciopen.com/article_pdf/1776880491481874433.pdf" /> <meta name="citation_publisher" content="中国科学院微生物研究所" /> <meta name="citation_fulltext_world_readable" content="" /> <meta name="Description" content="<p><i>Saccharolobus islandicus</i> REY15A represents one of the very few archaeal models with versatile genetic tools, which include efficient genome editing, gene silencing, and robust protein expression systems. However, plasmid vectors constructed for this crenarchaeon thus far are based solely on the pRN2 cryptic plasmid. Although this plasmid coexists with pRN1 in its original host, early attempts to test pRN1‐based vectors consistently failed to yield any stable host–vector system for <i>Sa. islandicus</i>. We hypothesized that this failure could be due to the occurrence of CRISPR immunity against pRN1 in this archaeon. We identified a putative target sequence in <i>orf904</i> encoding a putative replicase on pRN1 (target N1). Mutated targets (N1a, N1b, and N1c) were then designed and tested for their capability to escape the host CRISPR immunity by using a plasmid interference assay. The results revealed that the original target triggered CRISPR immunity in this archaeon, whereas all three mutated targets did not, indicating that all the designed target mutations evaded host immunity. These mutated targets were then incorporated into <i>orf904</i> individually, yielding corresponding mutated pRN1 backbones with which shuttle plasmids were constructed (pN1aSD, pN1bSD, and pN1cSD). <i>Sa. islandicus</i> transformation revealed that pN1aSD and pN1bSD were functional shuttle vectors, but pN1cSD lost the capability for replication. These results indicate that the missense mutations in the conserved helicase domain in pN1c inactivated the replicase. We further showed that pRN1‐based and pRN2‐based vectors were stably maintained in the archaeal cells either alone or in combination, and this yielded a dual plasmid system for genetic study with this important archaeal model.</p>" /> <meta name="article_references" content="Zhao P, Bi X, Wang X, et al. . 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/> </svg> <span>Cite</span> <div class="download-boxs"> <a href="/article/download_ris?tag=2&id=1776880491481874433" target="_blank" class="item-box"> EndNote(RIS) </a> <a href="/article/download_ris?tag=3&id=1776880491481874433" target="_blank" class="item-box"> BibTeX </a> </div> </div>  <div class="v4-art-top-item" id="collection_not_1776880491481874433" onclick="openCheck("1776880491481874433")"> <svg class="vt-art-top-item-img" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" width="22" height="22" viewBox="0 0 200 200"> <image id="图层_1" data-name="图层 1" x="18" y="18" width="164" height="164" 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/> </svg> <span>Share</span> <div class="baidu-share" id="yx_share"></div> </div>  <a class="v4-art-top-item" href="https://mc.manuscriptcentral.com/mlife" target="_blank"> <svg class="vt-art-top-item-img" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink" width="22" height="22" viewBox="0 0 200 200"> <image id="图层_1" data-name="图层 1" x="18" y="14" width="164" height="173" 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/> </svg> <span>Submit Manuscript</span> </a>  <el-button type="text" class="v4-art-top-item" style="padding: 4px 0" :disabled="aiChat.aiDrawerShow" @click="openAiChatDrawer"> <img src="/assets/img/article/AM_Full.svg" /> <span>AI Chat Paper</span> </el-button> </div> <div v-if="treeDataReady && treeData.length > 0 || imgMenuList.length>0 || tableMenuList.length>0" @click="drawer = true" class="v4-art-main-left-btn" id="v4_art_main_left_btn"> Show Outline </div> </div> </div> <el-drawer :size="300" class="v4-art-main-left-btn" :withHeader="false" :direction="'ltr'" :visible.sync="drawer"> <div class="v4-art-main-left" style="padding-top: 0"> <div v-if="treeDataReady && treeData.length > 0" style="border-bottom: 1px solid #eef"> <div class="v4-art-main-left-title">Outline</div> <el-tree ref="tree" node-key="id" :empty-text="''" :data="treeData" :indent="10" :highlight-current="true" :expand-on-click-node="false" :props="defaultProps" @node-click="handleNodeClick" > <span 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class="v4-show-hide-icon-out is-not-active v4_art_outline_show" @click="showOutline(1)">Show full outline <i class="el-icon-arrow-right"></i></div> <div class="v4-show-hide-icon-out is-active v4_art_outline_hide" @click="showOutline(2)">Hide outline <i class="el-icon-arrow-up"></i></div> </div> </div> <div class="v4-art-main-center"> <div class="art-html-center"> <div class="art-html-top"> <div class="v4-art-info">  <span>Original Research</span> <span style="font-family: auto" >|</span >   <span class="v4-art-info-oa">Open Access </span>  </div>  <h1 class="v4-ahc-title">Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for <i>Saccharolobus islandicus</i></h1>  <div> <span class="art-authors" ><span>Pengpeng Zhao</span><sup></sup ><span>, </span></span ><span class="art-authors" ><span>Xiaonan Bi</span><sup></sup ><span>, </span></span ><span class="art-authors" ><span>Xiaoning Wang</span><sup></sup ><span>, </span></span ><span class="art-authors" ><span>Xu Feng</span><sup></sup ><span>, </span></span ><span class="art-authors" ><span>Yulong Shen</span><sup></sup ><span>, </span></span ><span class="art-authors" ><span><a href='/scholar/info?id=1776880492916326402'>Guanhua Yuan</a></span><sup></sup ><a href="https://orcid.org/0000-0002-4448-6669" target="_blank" > <img style="width: 14px; display: inline-block" src="/assets/img/article/orcid.svg" /></a ><span class="art-authors-email" style="padding-left: 3px" >(<a onclick="send_email("1776880492916326402","1776880491481874433")"> <el-tooltip popper-class="v4-el-tooltip" effect="dark" content="yuangh@sdu.edu.cn" placement="top-start"> <img src="/assets/img/article/article-email.svg" style="width: 20px" /> </el-tooltip> </a >)</span ><span>, </span></span ><span class="art-authors" ><span><a href='/scholar/info?id=1776880492916326402'>Qunxin She</a></span><sup></sup ><a href="https://orcid.org/0000-0002-4448-6669" target="_blank" > <img style="width: 14px; display: inline-block" src="/assets/img/article/orcid.svg" /></a ><span class="art-authors-email" style="padding-left: 3px" >(<a onclick="send_email("1776880492916326402","1776880491481874433")"> <el-tooltip popper-class="v4-el-tooltip" effect="dark" content="yuangh@sdu.edu.cn" placement="top-start"> <img src="/assets/img/article/article-email.svg" style="width: 20px" /> </el-tooltip> </a >)</span ></span > </div> <div style="clear: both"></div> <div class="art-authors-unit art-authors-unit2"> <div class="art-authors-unit-inner art-authors-unit-inner2"> <div class="art-authors-unit-item"><addr-line>CRISPR and Archaea Biology Research Center, State Key Laboratory of Microbial Technology and Microbial Technology Institute, Shandong University, Qingdao, China</addr-line></div> <div class="art-authors-unit-item"><p><bold>Editor:</bold> Hua Xiang, Institute of Microbiology, Chinese Academy of Sciences, China</p></div> <div class="art-authors-unit-item"><p><sup>#</sup>Pengpeng Zhao and Xiaonan Bi contributed equally to this study.</p></div> </div> </div> <div> <span class="art-authors art-authors-more art-authors-more2" onclick="showAuthUnit(3)"> Show Author Information <i class="el-icon-caret-bottom"></i> </span> <span class="art-authors art-authors-less art-authors-less2" style="display: none" onclick="showAuthUnit(4)"> Hide Author Information <i class="el-icon-caret-top"></i> </span> </div>  <div style="border-top: 1px solid #eef; margin-top: 20px; padding-bottom: 8px" ></div>  <div class="v4-art-content-p"> <h2></h2> </div>   <div class="v4-art-content-p v4-art-abstract" id="title_-2"> <h2>Abstract</h2> <div><p><i>Saccharolobus islandicus</i> REY15A represents one of the very few archaeal models with versatile genetic tools, which include efficient genome editing, gene silencing, and robust protein expression systems. However, plasmid vectors constructed for this crenarchaeon thus far are based solely on the pRN2 cryptic plasmid. Although this plasmid coexists with pRN1 in its original host, early attempts to test pRN1‐based vectors consistently failed to yield any stable host–vector system for <i>Sa. islandicus</i>. We hypothesized that this failure could be due to the occurrence of CRISPR immunity against pRN1 in this archaeon. We identified a putative target sequence in <i>orf904</i> encoding a putative replicase on pRN1 (target N1). Mutated targets (N1a, N1b, and N1c) were then designed and tested for their capability to escape the host CRISPR immunity by using a plasmid interference assay. The results revealed that the original target triggered CRISPR immunity in this archaeon, whereas all three mutated targets did not, indicating that all the designed target mutations evaded host immunity. These mutated targets were then incorporated into <i>orf904</i> individually, yielding corresponding mutated pRN1 backbones with which shuttle plasmids were constructed (pN1aSD, pN1bSD, and pN1cSD). <i>Sa. islandicus</i> transformation revealed that pN1aSD and pN1bSD were functional shuttle vectors, but pN1cSD lost the capability for replication. These results indicate that the missense mutations in the conserved helicase domain in pN1c inactivated the replicase. We further showed that pRN1‐based and pRN2‐based vectors were stably maintained in the archaeal cells either alone or in combination, and this yielded a dual plasmid system for genetic study with this important archaeal model.</p></div> </div>  <div class="v4-art-content-p" id="title_-3"> <h2>Keywords</h2> <div class="v4-art-keyword"> <a class="v4-art-keyword-item" onclick="jumpKeyword("archaeal genetics")" >archaeal genetics</a ><a class="v4-art-keyword-item" onclick="jumpKeyword("CRISPR\u2010Cas")" >CRISPR‐Cas</a ><a class="v4-art-keyword-item" onclick="jumpKeyword("CRISPR escape mutations")" >CRISPR escape mutations</a ><a class="v4-art-keyword-item" onclick="jumpKeyword("dual plasmid vectors")" >dual plasmid vectors</a ><a class="v4-art-keyword-item" onclick="jumpKeyword("<i>Saccharolobus\u2013E. coli<\/i> shuttle vectors")" ><i>Saccharolobus–E. coli</i> shuttle vectors</a > </div> </div>   <div id="title_-4" class="v4-art-content-p" style="display: none"></div> <div style="border-top: 1px solid #eef; margin-top: 20px"> <div id="article_no_data" style="padding-top: 30px; display: none"> <p>No abstract is available for this article. 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NIH Image to ImageJ: 25 years of image analysis. <i>Nat Methods</i>. 2012;9:671–5.</p></span> </div> </div> <div class="v4-art-reference-item-pos"> <a target="_blank" href="https://doi.org/10.1038/nmeth.2089">Crossref</a> <a target="_blank" style="margin-left: 10px" href="https://scholar.google.com/scholar_lookup?title=NIH Image to ImageJ: 25 years of image analysis&journal=Nat Methods&volume=9&pages=671-5&publication_year=2012&author=Schneider CA&author=Rasband WS&author=Eliceiri KW">Google Scholar</a> </div> </div> </div> </div> </div> </div> </div> </div> <div class="v4-art-main-right"> <img class="v4-article-journal-img" src="/journal/get_journal_cover?flag=2&status=0&id=10.1002/mlf2.12107" /> <a class="v4-article-journal-title" href="/journal/2097-1699">mLife</a> <div class="v4-art-main-right-content"> <a href="/journal/join_journal/stage_page?stage=5&id=1685823922959323138&issueIndex=1776880604019245057&issn=2097-1699" >Volume 3 Issue 1,</br>March 2024</a> </div> <div class="v4-art-main-right-content"> <span>Pages 119-128</span> </div> <div class="v4-art-main-right-content"> DOI: <a href="https://doi.org/10.1002/mlf2.12107" target="_blank" style="word-break: break-all">10.1002/mlf2.12107</a> </div> <div class="v4-art-main-right-title">Cite this article:</div> <div class="v4-art-main-right-content"> <span>Zhao P, Bi X, Wang X, et al. </span> <span>Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for <i>Saccharolobus islandicus</i>. </span> <span><span style = 'font-style: italic;'>mLife</span>, </span> <span> 2024, 3(1): 119-128. </span> <span style="word-break: break-all">https://doi.org/10.1002/mlf2.12107</span> </div> <div class="v4-art-main-right-content"> </div> <el-collapse style="margin-top: 15px"> <el-collapse-item title="Metrics & Citations" name="4"> <template slot="title"> Metrics & Citations   <el-tooltip popper-class="v4-el-tooltip" effect="dark" content="Citation counts are pulled independently from Crossref, Web of Science, Scopus and CSCD. 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var authors = {"authors":[{"id":null,"name":"Pengpeng Zhao","email":null,"type":2,"companyId":null,"orcid":null,"firstAuthor":null,"sub":""},{"id":null,"name":"Xiaonan Bi","email":null,"type":2,"companyId":null,"orcid":null,"firstAuthor":null,"sub":""},{"id":null,"name":"Xiaoning Wang","email":null,"type":2,"companyId":null,"orcid":null,"firstAuthor":null,"sub":""},{"id":null,"name":"Xu Feng","email":null,"type":2,"companyId":null,"orcid":null,"firstAuthor":null,"sub":""},{"id":null,"name":"Yulong Shen","email":null,"type":2,"companyId":null,"orcid":null,"firstAuthor":null,"sub":""},{"id":"1776880492916326402","name":"<a href='\/scholar\/info?id=1776880492916326402'>Guanhua Yuan<\/a>","email":"yuangh@sdu.edu.cn","type":1,"companyId":null,"orcid":"0000-0002-4448-6669","firstAuthor":null,"sub":""},{"id":"1776880492916326402","name":"<a href='\/scholar\/info?id=1776880492916326402'>Qunxin She<\/a>","email":"yuangh@sdu.edu.cn","type":1,"companyId":null,"orcid":"0000-0002-4448-6669","firstAuthor":null,"sub":""}],"companys":["<addr-line>CRISPR and Archaea Biology Research Center, State Key Laboratory of Microbial Technology and Microbial Technology Institute, Shandong University, Qingdao, China<\/addr-line>","<p><bold>Editor:<\/bold> Hua Xiang, Institute of Microbiology, Chinese Academy of Sciences, China<\/p>","<p><sup>#<\/sup>Pengpeng Zhao and Xiaonan Bi contributed equally to this study.<\/p>"]}; var esmSize = 0; var articleId = "1776880491481874433"; var article = {"id":"1776880491481874433","title":"Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for <i>Saccharolobus islandicus<\/i>","doi":"10.1002\/mlf2.12107","type":"1407605528143134722","journalId":"1685823922959323138","isOa":1,"clickNum":167,"downloadNum":2,"collectionNum":null,"cscdNum":0,"quoteNum":1,"articleNo":"mlife-3-1-119","cover":null,"receivedTime":"2023-09-07T00:00:00.000+08:00","strReceivedTime":"07 September 2023","acceptedTime":"2023-11-25T00:00:00.000+08:00","strAcceptedTime":"25 November 2023","erratumId":null,"issueInfo":null,"issueInfoUrl":"<a href='\/journal\/join_journal\/archive?journalId=1685823922959323138\u0026volume=2024\u0026issn2097-1699'>2024, 3<\/a>(<a href='\/journal\/join_journal\/stage_page?stage=5\u0026id=1685823922959323138\u0026issueIndex=1776880604019245057\u0026issn2097-1699'>1<\/a>): 119-128","stageNum":5,"strType":"Original Research","strStage":"Issue","pubTime":"2024-03-20T00:00:01.000+08:00","pubYear":2024,"strPubTime":"20 March 2024","citationDate":"2024\/03\/01","erratum":null,"abstraction":"<p><i>Saccharolobus islandicus<\/i> REY15A represents one of the very few archaeal models with versatile genetic tools, which include efficient genome editing, gene silencing, and robust protein expression systems. However, plasmid vectors constructed for this crenarchaeon thus far are based solely on the pRN2 cryptic plasmid. Although this plasmid coexists with pRN1 in its original host, early attempts to test pRN1\u2010based vectors consistently failed to yield any stable host\u2013vector system for <i>Sa. islandicus<\/i>. We hypothesized that this failure could be due to the occurrence of CRISPR immunity against pRN1 in this archaeon. We identified a putative target sequence in <i>orf904<\/i> encoding a putative replicase on pRN1 (target N1). Mutated targets (N1a, N1b, and N1c) were then designed and tested for their capability to escape the host CRISPR immunity by using a plasmid interference assay. The results revealed that the original target triggered CRISPR immunity in this archaeon, whereas all three mutated targets did not, indicating that all the designed target mutations evaded host immunity. These mutated targets were then incorporated into <i>orf904<\/i> individually, yielding corresponding mutated pRN1 backbones with which shuttle plasmids were constructed (pN1aSD, pN1bSD, and pN1cSD). <i>Sa. islandicus<\/i> transformation revealed that pN1aSD and pN1bSD were functional shuttle vectors, but pN1cSD lost the capability for replication. These results indicate that the missense mutations in the conserved helicase domain in pN1c inactivated the replicase. We further showed that pRN1\u2010based and pRN2\u2010based vectors were stably maintained in the archaeal cells either alone or in combination, and this yielded a dual plasmid system for genetic study with this important archaeal model.<\/p>","abstractImage":null,"abstractToc":null,"acknowledgement":"<title>ACKNOWLEDGMENTS<\/title> <p>We thank members of CRISPR and Archaea Biology Research Center at Shandong University for helpful discussions. This research was funded by the National Key R\u0026D Program of China (Grant No. 2020YFA0906800 to Q. S.) and the National Natural Science Foundation of China (Nos. 32270040 to Q. S., 32001022 to X. F., and 32370033 to Y. S.).<\/p>","copyright":"\u00A9 2024 The Authors. mLife published by John Wiley \u0026 Sons Australia, Ltd on behalf of Institute of Microbiology, Chinese Academy of Sciences.","pdfSize":"(1.4 MB)","isCollection":false,"journalIssn":"2097-1699","issueId":"1776880604019245057","revised":null,"issueDate":" March 2024","serviceAddress":null,"price":0.0,"subject":null,"subjects":null,"license":"<p>This is an open access article under the terms of the <a ext-link-type=\"uri\" href=\"http:\/\/creativecommons.org\/licenses\/by\/4.0\/\">Creative Commons Attribution<\/a> License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.<\/p>","ifErratum":false,"webOfScienceNum":1,"scopusNum":1,"trendMd":null,"trendMdHtml":null,"articleRecommendVos":null,"issue":"1","volume":"3","page":"Pages 119-128","citationPublicationDate":"2024\/3\/20","issueTime":"2024\/3\/1","authorAndCompanyVOS":[{"author":"Pengpeng Zhao","company":" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. "},{"author":"Xiaonan Bi","company":" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. "},{"author":"Xiaoning Wang","company":" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. "},{"author":"Xu Feng","company":" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. "},{"author":"Yulong Shen","company":" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. "},{"author":"Guanhua Yuan","company":" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. "},{"author":"Qunxin She","company":" CRISPR and Archaea Biology Research Center,CHINA. State Key Laboratory of Microbial Technology and Microbial Technology Institute,CHINA. Shandong University, 266237,CHINA. "}],"citationAuthorInstitution":null,"journalOrgan":"\u4E2D\u56FD\u79D1\u5B66\u9662\u5FAE\u751F\u7269\u7814\u7A76\u6240","altmetricTwo":null,"keypointsAbstracted":null,"isMerge":null,"articleCiteVO":{"author":"Zhao P, Bi X, Wang X, et al. ","title":"Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for <i>Saccharolobus islandicus<\/i>. ","journal":"<span style = 'font-style: italic;'>mLife<\/span>, ","volume":" 2024, 3(1): 119-128. ","doi":"https:\/\/doi.org\/10.1002\/mlf2.12107","allStr":"Zhao P, Bi X, Wang X, et al. . Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for <i>Saccharolobus islandicus<\/i>. . <span style = 'font-style: italic;'>mLife<\/span>, . 2024, 3(1): 119-128. . https:\/\/doi.org\/10.1002\/mlf2.12107"},"frontNotesVO":{"title":null,"content":null},"articleCode":"","articleCodeId":null,"clcNumber":null,"documentsCode":null,"jumpIssueId":"1776880604019245057","jel":null,"mergeId":null,"fpage":"119","lpage":"128"}; var uuid = "qk_088bd8f43d5d4441b80504ca0800f19f"; var referenceList = [{"id":"1777168973469257730","articleId":null,"reference":"<p>Woese CR, Fox GE. 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Diverse CRISPR\u2010Cas responses and dramatic cellular DNA changes and cell death in pKEF9\u2010conjugated <i>Sulfolobus<\/i> species. <i>Nucleic Acids Res<\/i>. 2016;44:4233\u201342.<\/p>","keyStr":"b65","label":"65","content":null,"num":null,"doi":"10.1093\/nar\/gkw286","link":"https:\/\/scholar.google.com\/scholar_lookup?title=Diverse CRISPR\u2010Cas responses and dramatic cellular DNA changes and cell death in pKEF9\u2010conjugated Sulfolobus species\u0026journal=Nucleic Acids Res\u0026volume=44\u0026pages=4233-42\u0026publication_year=2016\u0026author=Liu G\u0026author=She Q\u0026author=Garrett RA"},{"id":"1777168973670584321","articleId":null,"reference":"<p>Manica A, Zebec Z, Steinkellner J, Schleper C. Unexpectedly broad target recognition of the CRISPR\u2010mediated virus defence system in the archaeon <i>Sulfolobus solfataricus<\/i>. <i>Nucleic Acids Res<\/i>. 2013;41:10509\u201317.<\/p>","keyStr":"b66","label":"66","content":null,"num":null,"doi":"10.1093\/nar\/gkt767","link":"https:\/\/scholar.google.com\/scholar_lookup?title=Unexpectedly broad target recognition of the CRISPR\u2010mediated virus defence system in the archaeon Sulfolobus solfataricus\u0026journal=Nucleic Acids Res\u0026volume=41\u0026pages=10509-17\u0026publication_year=2013\u0026author=Manica A\u0026author=Zebec Z\u0026author=Steinkellner J\u0026author=Schleper C"},{"id":"1777168973674778625","articleId":null,"reference":"<p>Schleper C, Kubo K, Zillig W. The particle SSV1 from the extremely thermophilic archaeon <i>Sulfolobus<\/i> is a virus: demonstration of infectivity and of transfection with viral DNA. <i>Proc Natl Acad Sci USA<\/i>. 1992;89:7645\u20139.<\/p>","keyStr":"b67","label":"67","content":null,"num":null,"doi":"10.1073\/pnas.89.16.7645","link":"https:\/\/scholar.google.com\/scholar_lookup?title=The particle SSV1 from the extremely thermophilic archaeon Sulfolobus is a virus: demonstration of infectivity and of transfection with viral DNA\u0026journal=Proc Natl Acad Sci USA\u0026volume=89\u0026pages=7645-9\u0026publication_year=1992\u0026author=Schleper C\u0026author=Kubo K\u0026author=Zillig W"},{"id":"1777168973678972929","articleId":null,"reference":"<p>Berkner S, Hinojosa MP, Prangishvili D, Lipps G. 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The emerging role of recombineering in microbiology. <i>Eng Microbiol<\/i>. 2023;3:100097.<\/p>","keyStr":"b70","label":"70","content":null,"num":null,"doi":"10.1016\/j.engmic.2023.100097","link":"https:\/\/scholar.google.com\/scholar_lookup?title=The emerging role of recombineering in microbiology\u0026journal=Eng Microbiol\u0026volume=3\u0026pages=100097\u0026publication_year=2023\u0026author=Li R\u0026author=Li A\u0026author=Zhang Y\u0026author=Fu J"},{"id":"1777168973687361538","articleId":null,"reference":"<p>She Q, Singh RK, Confalonieri F, Zivanovic Y, Allard G, Awayez MJ, et al. The complete genome of the crenarchaeon <i>Sulfolobus solfataricus<\/i> P2. <i>Proc Natl Acad Sci USA<\/i>. 2001;98:7835\u201340.<\/p>","keyStr":"b71","label":"71","content":null,"num":null,"doi":"10.1073\/pnas.141222098","link":"https:\/\/scholar.google.com\/scholar_lookup?title=The complete genome of the crenarchaeon Sulfolobus solfataricus P2\u0026journal=Proc Natl Acad Sci USA\u0026volume=98\u0026pages=7835-40\u0026publication_year=2001\u0026author=She Q\u0026author=Singh RK\u0026author=Confalonieri F\u0026author=Zivanovic Y\u0026author=Allard G\u0026author=Awayez MJ"},{"id":"1777168973687361539","articleId":null,"reference":"<p>Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. <i>Nat Methods<\/i>. 2012;9:671\u20135.<\/p>","keyStr":"b72","label":"72","content":null,"num":null,"doi":"10.1038\/nmeth.2089","link":"https:\/\/scholar.google.com\/scholar_lookup?title=NIH Image to ImageJ: 25 years of image analysis\u0026journal=Nat Methods\u0026volume=9\u0026pages=671-5\u0026publication_year=2012\u0026author=Schneider CA\u0026author=Rasband WS\u0026author=Eliceiri KW"}]; var ifShowFullText = false; var galleryTopTable; var galleryThumbsTable; //设置预览全局变量 var ifPreview = 0 || 0; // 在《纳米研究》2024年Issue2中35篇文章的下载按钮上增加Google下载事件代码 var ifNeedGoogleDownload = 1; // 是否需要显示show all outline按钮 var allOutlineShow = false; // 在《纳米研究》2024年Issue2中35篇文章的下载按钮上增加Google下载事件代码 function googleDownload() { if (ifNeedGoogleDownload == 0) { gtag('event', 'conversion', { 'send_to': 'AW-10902746976/k8LBCKGMg5YZEODu6s4o' }); } } // 正文左侧导航的节点 var domArr = []; var treeDataO = []; var articleApp = new Vue({ el: '#article-app', data: { 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generators were installed on sloping buildings such as houses to achieve large-scale raindrop energy harvesting easily. Reprinted with permission from <i>iEnergy</i>, 2(2): 93–99, 2023 © 2023 The Author(s)</p>\n</fig>", // "contentHtml": "<img id=\"image-iEng-2023-s-0024-1\" onclick=\"showImage(event,this)\" 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original-src=\"https://wqketang.oss-cn-beijing.aliyuncs.com/zip-unzip/zip-7f9b22ae-5865-4143-a681-f77a8724f8dd/iEng-2023-s-0024/files/iEng-2023-s-0024-1.jpg?Expires=1719580654&OSSAccessKeyId=STS.NUbZf2DD6Ue3FqTmGvgkvUbF1&Signature=CaKJgAJ16yzHNf6wFEX2G1f1LT0%3D&security-token=CAISywJ1q6Ft5B2yfSjIr5bXEdyGqZsX4qfYRFfliUcja%2BRauqft0zz2IHtKenhsBOsbtfk1mG5W5%2FgZlqJ9SptIAEfJa9d99Mz%2Bf8BIjdCT1fau5Jko1beHewHKeTOZsebWZ%2BLmNqC%2FHt6md1HDkAJq3LL%2Bbk%2FMdle5MJqP%2B%2FUFB5ZtKWveVzddA8pMLQZPsdITMWCrVcygKRn3mGHdfiEK00he8TouufTinpHMskGA1Aell7Mvyt6vcsT%2BXa5FJ4xiVtq55utye5fa3TRYgxowr%2Fwo0v0YpGya5YzHXwcPskvdKZbo78UqLQlla6w%2BGqFJqvPxr%2Fp8t%2Fx5fWJKAezhVgs8cVM8JOjIqKOscIsiZmYk4pM4wgzSyCaJL7f%2FhREKa7znWGyxgyLY25K9yOXNh%2FA7x25WFZknm%2BbJoNLmr0pOOvEupyi0697UTzDnGoABihsmGXKWpSd6XDZIstjWbPKKorqYDJSMThEt%2F%2F9EPJXR4F4eCQ9BJV3gUyzfY08MnHKQ4H7mG8WnZ8%2FjZiwrfF7NSWXWD8SUf46hbaSsKbigUEcuiHoLO8%2BpSp%2BiEOlDNxxej3LE4vOpbeaqnNSNZFXt3nMTNC1HaeHqNJS7lYcgAA%3D%3D\">\n<p style=\"text-align:center\"><label>Fig. 1</label></p>", // "htmlWithNoLabel": "<img src=\"zip-unzip/zip-7f9b22ae-5865-4143-a681-f77a8724f8dd/iEng-2023-s-0024/files/iEng-2023-s-0024-1.jpg\" />", // "parentHtml": "<fig id=\"Figure1\">\n <img src=\"https://wqketang.oss-cn-beijing.aliyuncs.com/zip-unzip/zip-7f9b22ae-5865-4143-a681-f77a8724f8dd/iEng-2023-s-0024/files/iEng-2023-s-0024-1.jpg?Expires=1719580654&OSSAccessKeyId=STS.NUbZf2DD6Ue3FqTmGvgkvUbF1&Signature=AJ%2BQ6vjArPRI%2FXGsFthpTKeluio%3D&x-oss-process=image%2Fresize%2Cm_fixed%2Cw_217&security-token=CAISywJ1q6Ft5B2yfSjIr5bXEdyGqZsX4qfYRFfliUcja%2BRauqft0zz2IHtKenhsBOsbtfk1mG5W5%2FgZlqJ9SptIAEfJa9d99Mz%2Bf8BIjdCT1fau5Jko1beHewHKeTOZsebWZ%2BLmNqC%2FHt6md1HDkAJq3LL%2Bbk%2FMdle5MJqP%2B%2FUFB5ZtKWveVzddA8pMLQZPsdITMWCrVcygKRn3mGHdfiEK00he8TouufTinpHMskGA1Aell7Mvyt6vcsT%2BXa5FJ4xiVtq55utye5fa3TRYgxowr%2Fwo0v0YpGya5YzHXwcPskvdKZbo78UqLQlla6w%2BGqFJqvPxr%2Fp8t%2Fx5fWJKAezhVgs8cVM8JOjIqKOscIsiZmYk4pM4wgzSyCaJL7f%2FhREKa7znWGyxgyLY25K9yOXNh%2FA7x25WFZknm%2BbJoNLmr0pOOvEupyi0697UTzDnGoABihsmGXKWpSd6XDZIstjWbPKKorqYDJSMThEt%2F%2F9EPJXR4F4eCQ9BJV3gUyzfY08MnHKQ4H7mG8WnZ8%2FjZiwrfF7NSWXWD8SUf46hbaSsKbigUEcuiHoLO8%2BpSp%2BiEOlDNxxej3LE4vOpbeaqnNSNZFXt3nMTNC1HaeHqNJS7lYcgAA%3D%3D\" original-src=\"https://wqketang.oss-cn-beijing.aliyuncs.com/zip-unzip/zip-7f9b22ae-5865-4143-a681-f77a8724f8dd/iEng-2023-s-0024/files/iEng-2023-s-0024-1.jpg?Expires=1719580654&OSSAccessKeyId=STS.NUbZf2DD6Ue3FqTmGvgkvUbF1&Signature=CaKJgAJ16yzHNf6wFEX2G1f1LT0%3D&security-token=CAISywJ1q6Ft5B2yfSjIr5bXEdyGqZsX4qfYRFfliUcja%2BRauqft0zz2IHtKenhsBOsbtfk1mG5W5%2FgZlqJ9SptIAEfJa9d99Mz%2Bf8BIjdCT1fau5Jko1beHewHKeTOZsebWZ%2BLmNqC%2FHt6md1HDkAJq3LL%2Bbk%2FMdle5MJqP%2B%2FUFB5ZtKWveVzddA8pMLQZPsdITMWCrVcygKRn3mGHdfiEK00he8TouufTinpHMskGA1Aell7Mvyt6vcsT%2BXa5FJ4xiVtq55utye5fa3TRYgxowr%2Fwo0v0YpGya5YzHXwcPskvdKZbo78UqLQlla6w%2BGqFJqvPxr%2Fp8t%2Fx5fWJKAezhVgs8cVM8JOjIqKOscIsiZmYk4pM4wgzSyCaJL7f%2FhREKa7znWGyxgyLY25K9yOXNh%2FA7x25WFZknm%2BbJoNLmr0pOOvEupyi0697UTzDnGoABihsmGXKWpSd6XDZIstjWbPKKorqYDJSMThEt%2F%2F9EPJXR4F4eCQ9BJV3gUyzfY08MnHKQ4H7mG8WnZ8%2FjZiwrfF7NSWXWD8SUf46hbaSsKbigUEcuiHoLO8%2BpSp%2BiEOlDNxxej3LE4vOpbeaqnNSNZFXt3nMTNC1HaeHqNJS7lYcgAA%3D%3D\">\n <p><label>Fig. 1</label>Bridge array generators were installed on sloping buildings such as houses to achieve large-scale raindrop energy harvesting easily. Reprinted with permission from <i>iEnergy</i>, 2(2): 93–99, 2023 © 2023 The Author(s)</p>\n</fig>", // "contentHtml": "<img id=\"image-iEng-2023-s-0024-1\" onclick=\"showImage(event,this)\" src=\"https://wqketang.oss-cn-beijing.aliyuncs.com/zip-unzip/zip-7f9b22ae-5865-4143-a681-f77a8724f8dd/iEng-2023-s-0024/files/iEng-2023-s-0024-1.jpg?Expires=1719580654&OSSAccessKeyId=STS.NUbZf2DD6Ue3FqTmGvgkvUbF1&Signature=%2BhRmDGIUtD9EkRxdDzgF%2FWU9dDE%3D&x-oss-process=image%2Fresize%2Cm_fixed%2Cw_600&security-token=CAISywJ1q6Ft5B2yfSjIr5bXEdyGqZsX4qfYRFfliUcja%2BRauqft0zz2IHtKenhsBOsbtfk1mG5W5%2FgZlqJ9SptIAEfJa9d99Mz%2Bf8BIjdCT1fau5Jko1beHewHKeTOZsebWZ%2BLmNqC%2FHt6md1HDkAJq3LL%2Bbk%2FMdle5MJqP%2B%2FUFB5ZtKWveVzddA8pMLQZPsdITMWCrVcygKRn3mGHdfiEK00he8TouufTinpHMskGA1Aell7Mvyt6vcsT%2BXa5FJ4xiVtq55utye5fa3TRYgxowr%2Fwo0v0YpGya5YzHXwcPskvdKZbo78UqLQlla6w%2BGqFJqvPxr%2Fp8t%2Fx5fWJKAezhVgs8cVM8JOjIqKOscIsiZmYk4pM4wgzSyCaJL7f%2FhREKa7znWGyxgyLY25K9yOXNh%2FA7x25WFZknm%2BbJoNLmr0pOOvEupyi0697UTzDnGoABihsmGXKWpSd6XDZIstjWbPKKorqYDJSMThEt%2F%2F9EPJXR4F4eCQ9BJV3gUyzfY08MnHKQ4H7mG8WnZ8%2FjZiwrfF7NSWXWD8SUf46hbaSsKbigUEcuiHoLO8%2BpSp%2BiEOlDNxxej3LE4vOpbeaqnNSNZFXt3nMTNC1HaeHqNJS7lYcgAA%3D%3D\">", // "name": "graphic" // } // ], // "imgKeyList": [ // "image-iEng-2023-s-0024-1" // ], // "tabKeyList": [], // "tables": [], // "fullTextUrl": "https://wqketang.oss-cn-beijing.aliyuncs.com/other/journal_prod/2024-06-28/3098b740-7b52-44b7-88a9-92fedc8988db.gif?Expires=1719560854&OSSAccessKeyId=STS.NUbZf2DD6Ue3FqTmGvgkvUbF1&Signature=1K7ZtLBP2mEeczlPMrOHw4aoGus%3D&security-token=CAISywJ1q6Ft5B2yfSjIr5bXEdyGqZsX4qfYRFfliUcja%2BRauqft0zz2IHtKenhsBOsbtfk1mG5W5%2FgZlqJ9SptIAEfJa9d99Mz%2Bf8BIjdCT1fau5Jko1beHewHKeTOZsebWZ%2BLmNqC%2FHt6md1HDkAJq3LL%2Bbk%2FMdle5MJqP%2B%2FUFB5ZtKWveVzddA8pMLQZPsdITMWCrVcygKRn3mGHdfiEK00he8TouufTinpHMskGA1Aell7Mvyt6vcsT%2BXa5FJ4xiVtq55utye5fa3TRYgxowr%2Fwo0v0YpGya5YzHXwcPskvdKZbo78UqLQlla6w%2BGqFJqvPxr%2Fp8t%2Fx5fWJKAezhVgs8cVM8JOjIqKOscIsiZmYk4pM4wgzSyCaJL7f%2FhREKa7znWGyxgyLY25K9yOXNh%2FA7x25WFZknm%2BbJoNLmr0pOOvEupyi0697UTzDnGoABihsmGXKWpSd6XDZIstjWbPKKorqYDJSMThEt%2F%2F9EPJXR4F4eCQ9BJV3gUyzfY08MnHKQ4H7mG8WnZ8%2FjZiwrfF7NSWXWD8SUf46hbaSsKbigUEcuiHoLO8%2BpSp%2BiEOlDNxxej3LE4vOpbeaqnNSNZFXt3nMTNC1HaeHqNJS7lYcgAA%3D%3D", // "glossary": [], // "notes": [], // "fundingStatements": [], // "app": [], // "fn": [], // "bio": [] // }; if (fullTextInfo['glossary']['length'] > 0) { for (var gl = 0; gl < fullTextInfo['glossary']['length']; gl++) { treeDataO.push({ id: 'content_title_-4_' + gl, label: fullTextInfo['glossary'][gl]['title'] }); } } // 正文 if (fullTextInfo['trees'].length > 0) { fullTextInfo['trees'].forEach(item => { if (item.key !== 'esm1') { var nodeC = { id: 'content_' + item.key, label: item.title, children: [] }; if (item.children && item.children.length > 0) { allOutlineShow = true; item.children.forEach(child => { if (child.key !== 'esm1') { var nodeS = { id: 'content_' + child.key, label: child.title, children: [] }; if (child.children && child.children.length > 0) { child.children.forEach(son => { if (son.key !== 'esm1') { nodeS.children.push({ id: 'content_' + son.key, label: son.title, children: [] }); } }); } nodeC.children.push(nodeS); } }); } treeDataO.push(nodeC); } }); } // app 附录 if (fullTextInfo['app']['length'] > 0) { for (var ap = 0; ap < fullTextInfo['app']['length']; ap++) { treeDataO.push({ id: 'content_title_-5_' + ap, label: fullTextInfo['app'][ap]['title'] }); } } // fn 脚注 if (fullTextInfo['fn']['length'] > 0) { treeDataO.push({ id: 'content_title_-6', label: 'Annotation' }); } // esm if (article.strType !== 'Issue Information') { treeDataO.push({ id: 'content_title_-11', label: 'Electronic Supplementary Material' }); } // ack 致谢 if (article['acknowledgement']) { treeDataO.push({ id: 'content_title_-7', label: 'Acknowledgements' }); } // funding-statement 基金 if (fullTextInfo['fundingStatements']['length'] > 0) { treeDataO.push({ id: 'content_title_-8', label: 'Funding' }); } // notes [ 伦理声明,知情同意书, 利益声明,作者声明,数据声明] if (fullTextInfo['notes']['length'] > 0) { for (var no = 0; no < fullTextInfo['notes'].length; no++) { treeDataO.push({ id: 'content_title_-10_' + no, label: fullTextInfo['notes'][no]['title'] }); } } // 参考文献 if (referenceList && referenceList['length'] > 0) { treeDataO.push({ id: 'content_title_-12', label: 'References' }); } // bio 作者简介 if (fullTextInfo['bio']['length'] > 0) { treeDataO.push({ id: 'content_title_-13', label: 'Author biography' }); } // 大纲hover时显示title var reg = /<\/?.+?\/?>/g; treeDataO.forEach(item => { item.labelWord = item['label'].replace(reg, ''); if (item.children && item.children.length > 0) { item.children.forEach(child => { child.labelWord = child.label.replace(reg, ''); if (child.children && child.children.length > 0) { child.children.forEach(son => { son.labelWord = son.label.replace(reg, ''); }); } }); } }); articleApp.treeData = treeDataO; articleApp.imgMenuList = fullTextInfo['figImages']; articleApp.tableMenuList = fullTextInfo['tabKeyList']; //所有fig图片/大纲信息 imgKeyListInfo = fullTextInfo['imgKeyList']; // 正文所有的图片 imgListInfo = fullTextInfo['images']; // 可以点击看大图的图片 imgShowListInfo = []; if (imgListInfo.length > 0) { imgListInfo.forEach(item => { if (item['parentHtml'].indexOf('class="inline-graphic"') < 0) { imgShowListInfo.push(item); } }); // 缩略图大图查看 var imageBigHtml = ''; if (imgShowListInfo.length > 0) { for (var m = 0; m < imgShowListInfo.length; m++) { imageBigHtml = imageBigHtml + '<li>' + '<div>' + imgShowListInfo[m]['parentHtml'] + '</div>' + '</li>'; } } $('#content_img').html(imageBigHtml); } //表格大纲信息 tableListInfo = fullTextInfo['tables']; if (tableListInfo.length > 0) { // 表格查看大图 var tableBig1Html = ''; var tableBig2Html = ''; if (tableListInfo.length > 0) { for (var n = 0; n < tableListInfo.length; n++) { tableBig1Html = '<div class="swiper-slide" style="background-color: #fff">' + '<div class="ahc-content" style="max-height: 100%; width: 100%; padding: 20px; overflow: auto">' + tableListInfo[n]['parentHtml'] + '</div>' + '</div>'; tableBig2Html = '<div class="swiper-slide table-big-view" style="cursor: pointer">' + tableListInfo[n]['html'] + '</div>'; $('#insert_table_big_1').append(tableBig1Html); $('#insert_table_big_2').append(tableBig2Html); } } } // 正文信息 $.ajax({ url: fullTextInfo['fullTextUrl'], // url: 'http://localhost:8733/assets/fig-test.gif', type: 'get', success: function(data) { fullTextInfo['contentList'] = JSON.parse(data); // glossary if (fullTextInfo['glossary']['length'] > 0) { $('#title_-4').css('display', 'block'); var dom = ''; for (var g = 0; g < fullTextInfo['glossary']['length']; g++) { dom = dom + '<div class="v4-art-content-p">' + '<div class="glossary-item" id="title_-4_' + g + '">' + '<h2>' + fullTextInfo['glossary'][g]['title'] + '</h2>' + '<div>' + fullTextInfo['glossary'][g]['content'] + '</div>' + '</div>' + '</div>'; } dom = dom + '<div style="clear: both;"></div>'; $('#title_-4').html(dom); } // 正文 var contentHtml = ''; var contentHtml2 = ''; esmTextInfo = ''; esmTextTitle = ''; if (fullTextInfo['contentList'] && fullTextInfo['contentList']['length'] > 0) { for (var s = 0; s < fullTextInfo['contentList'].length; s++) { // 处理图片 for (var i = 0; i < imgListInfo['length']; i++) { const mergImg = new RegExp(`<img[^>]*id="${imgListInfo[i]['key']}"[^>]*>`, 'i'); if (imgListInfo[i]['contentHtml'].indexOf('class="inline-graphic"') < 0) { // 是否是fig图片 var ifFig = false; for (var ii = 0; ii < imgKeyListInfo.length; ii++) { if (imgListInfo[i]['contentHtml'].indexOf(imgKeyListInfo[ii]) > 0) { ifFig = true; } } if (ifFig) { fullTextInfo['contentList'][s]['content'] = fullTextInfo['contentList'][s]['content'].replace(mergImg, imgListInfo[i]['contentHtml'] + '<div class="v4-art-image">\n' + ' <span onclick="showImageBtn(event, \'' + imgListInfo[i]['key'] + '\')">View original image</span>\n' + ' <span onclick="downloadImageBtn(event, \'' + imgListInfo[i]['key'] + '\')">Download original image</span>\n' + ' </div>'); } else { fullTextInfo['contentList'][s]['content'] = fullTextInfo['contentList'][s]['content'].replace(mergImg, imgListInfo[i]['contentHtml']); } } else { fullTextInfo['contentList'][s]['content'] = fullTextInfo['contentList'][s]['content'].replace(mergImg, imgListInfo[i]['contentHtml']); } } // 处理正文的脚注内容，实现定位 const xrefFn = new RegExp(`ref-type="fn"`, 'gi'); fullTextInfo['contentList'][s]['content'] = fullTextInfo['contentList'][s]['content'].replace(xrefFn, 'ref-type="fn" onclick="findFnById(event)"'); // 正文内出现esm说明文字内容 if (fullTextInfo['contentList'][s]['key'] === 'esm1') { esmTextInfo = fullTextInfo['contentList'][s]['content']; esmTextTitle = fullTextInfo['contentList'][s]['title']; } else { contentHtml = contentHtml + '<div class="v4-art-content-p" id="' + fullTextInfo['contentList'][s]['key'] + '">' + '<h2 >' + fullTextInfo['contentList'][s]['title'] + '</h2>' + '<div style="overflow-x: auto; overflow-y: hidden;">' + fullTextInfo['contentList'][s]['content'] + '</div>' + '</div>'; } } } // app if (fullTextInfo['app']['length'] > 0) { for (var a = 0; a < fullTextInfo['app']['length']; a++) { contentHtml = contentHtml + '<div class="v4-art-content-p" id="title_-5_' + a + '">' + '<h2>' + fullTextInfo['app'][a]['label'] + fullTextInfo['app'][a]['title'] + '</h2>' + '<div style="overflow-x: auto; overflow-y: hidden;">' + fullTextInfo['app'][a]['content'] + '</div>' + '</div>'; } } // fn if (fullTextInfo['fn']['length'] > 0) { contentHtml = contentHtml + '<div class="v4-art-content-p" id="title_-6">' + '<h2 class="content-title">' + 'Annotation' + '</h2>'; for (var f = 0; f < fullTextInfo['fn']['length']; f++) { contentHtml = contentHtml + '<div class="fn-item">' + '<span style="cursor: pointer;" id="f_' + fullTextInfo['fn'][f]['key'] + '" onclick="findFnInContentById(this)">' + fullTextInfo['fn'][f]['title'] + '</span>' + '<div>' + fullTextInfo['fn'][f]['content'] + '</div>' + '</div>'; } contentHtml = contentHtml + '</div>'; } // 正文内的esmText放到esm位置 if (esmTextInfo) { if (esmSize > 0) { $('#esm_text').html(esmTextInfo); $('#esm_text').css('display', 'block'); $('#esm_title').html(esmTextTitle); } else if (esmSize < 0) { contentHtml = contentHtml + '<div class="v4-art-content-p" id="title_-11">' + '<h2 class="content-title">' + esmTextTitle + '</h2>' + '<div style="overflow-x: auto; overflow-y: hidden;">' + esmTextInfo + '</div>' + '</div>'; } treeDataO.forEach((item) => { if (item.id === 'content_title_-11') { item.label = esmTextTitle; item.labelWord = esmTextTitle; articleApp.treeData = treeDataO; } }); } else { if (esmSize === 0) { treeDataO.forEach((item, index) => { if (item.id === 'content_title_-11') { treeDataO.splice(index, 1); articleApp.treeData = treeDataO; } }); } } articleApp.treeDataReady = true; // ack if (article['acknowledgement']) { contentHtml2 = contentHtml2 + '<div class="v4-art-content-p" id="title_-7">' + '<h2 class="content-title">' + 'Acknowledgements' + '</h2>' + '<div style="overflow-x: auto; overflow-y: hidden;">' + article['acknowledgement'] + '</div>' + '</div>'; } // funding-statement if (fullTextInfo['fundingStatements']['length'] > 0) { contentHtml2 = contentHtml2 + '<div class="v4-art-content-p" id="title_-8">' + '<h2 class="content-title">' + 'Funding' + '</h2>'; for (var fs = 0; fs < fullTextInfo['fundingStatements']['length']; fs++) { contentHtml2 = contentHtml2 + '<div class="fs-item"><p>' + fullTextInfo['fundingStatements'][fs] + '</p></div>'; } contentHtml2 = contentHtml2 + '</div>'; } // notes if (fullTextInfo['notes']['length'] > 0) { contentHtml2 = contentHtml2 + '<div class="v4-art-content-p" id="title_-10">'; for (var n = 0; n < fullTextInfo['notes']['length']; n++) { contentHtml2 = contentHtml2 + '<div class="notes-item" id="title_-10_' + n + '">' + '<h2>' + fullTextInfo['notes'][n]['title'] + '</h2>' + '<div>' + fullTextInfo['notes'][n]['content'] + '</div>' + '</div>'; } contentHtml2 = contentHtml2 + '</div>'; } // bio var contentHtmlBio = ''; if (fullTextInfo['bio']['length'] > 0) { contentHtmlBio = contentHtmlBio + '<div class="v4-art-content-p" id="title_-13" style="min-height: 200px;">' + '<h2>' + 'Author biography' + '</h2>'; for (var b = 0; b < fullTextInfo['bio']['length']; b++) { contentHtmlBio = contentHtmlBio + '<div class="bio-item">' + (fullTextInfo['bio'][b]['path'] ? '<img src="' + fullTextInfo['bio'][b]['path'] + '">' : '') + '<div>' + fullTextInfo['bio'][b]['content'] + '</div>' + '</div>'; } contentHtmlBio = contentHtmlBio + '<div style="clear: both"></div></div>'; } $('#insert_content_one').prepend(contentHtml); $('#insert_content_two').prepend(contentHtml2); $('#insert_content').append(contentHtmlBio); setTimeout(function() { if ($('#insert_content .v4-art-content-p').last().height() < 500) { $('#insert_content .v4-art-content-p').last().css('padding-bottom', '500px'); } }, 1000); MathJax.Hub.Queue(['Typeset', MathJax.Hub]); // 渲染好数据之后，； setTimeout(function() { // 图片大图模态初始化 if (document.getElementById('content_img')) { preViewImage = new Viewer(document.getElementById('content_img'), { url: 'original-src', }); } // 表格大图查看初始化 initSwiper(); initTagClickHandler(); // 插入查看表格按钮如果是移动端去掉表格模态查看 => 屏幕小于600 去掉表格查看 if ($(window).width() > 600) { var tableDom = $('#insert_content table'); for (var ta = 0; ta < tableDom.length; ta++) { if ($($('#insert_content table')[ta]).attr('id')) { // 判断 if (tableListInfo.length > 0) { let haveT = false; for (let tt = 0; tt < tableListInfo.length; tt++) { if ($($('#insert_content table')[ta]).attr('id') == tableListInfo[tt]['key']) { haveT = true; } } if (haveT) { var b = document.createElement('div'); b.setAttribute('onclick', 'showTableBtn(this)'); b.setAttribute('class', 'v4-art-table'); b.innerText = 'Show table'; $($('#insert_content table')[ta]).parent().parent().append(b); } } } } } // 左侧大纲节点 domArr = $('.v4-art-content-p'); // 是否显示show outline setTimeout(() => { if (allOutlineShow) { $(".v4_art_outline_show").css('display', 'block'); } }, 300); }, 10); if ( fullTextInfo['glossary']['length'] === 0 && (!fullTextInfo['contentList'] || fullTextInfo['contentList']['length'] === 0) && fullTextInfo['app']['length'] === 0 && fullTextInfo['fn']['length'] === 0 && (!esmTextInfo && esmSize === 0) && !article['acknowledgement'] && fullTextInfo['fundingStatements']['length'] === 0 && fullTextInfo['notes']['length'] === 0 && fullTextInfo['bio']['length'] === 0 && referenceList['length'] === 0 && !article.clcNumber && !article.documentsCode && !article.articleCodeId && !article.abstraction && !article.abstractToc && !article.abstractImage && !article['keypointsAbstracted'] && !article.erratum && articleKeyword.length === 0 ) { $('#article_no_data').css('display', 'block'); } }, error: function(err) { alert(err.toString()); }, }); }, error: function(err) { alert(err.toString()); }, }); } // 进入页面时执行 // 组装大纲 // key-points Highlights keypointsAbstracted if (article['keypointsAbstracted'] != null) { treeDataO.push({ id: 'content_title_-1', label: article['keypointsAbstracted']['title'] }); } // 摘要 article.abstractImage != null if ((article.abstractImage != null && article.abstractImage != '') || (article.abstractToc != null && article.abstractToc != '')) { treeDataO.push({ id: 'content_title_-2-1', label: 'Graphical Abstract' }); } // 摘要 article.abstraction != null || (article.abstractToc != null && article.abstractToc != '') if (article.abstraction != null && article.abstraction != '') { treeDataO.push({ id: 'content_title_-2', label: 'Abstract' }); } // 关键词 if (articleKeyword.length>0) { treeDataO.push({ id: 'content_title_-3', label: 'Keywords' }); } // CLC number 2024-7-8 大纲中去掉CLC Number显示 // if ((article.clcNumber != null && article.clcNumber != '') || (article.documentsCode != null && article.documentsCode != '') // || (article.articleCodeId != null && article.articleCodeId != '')) { // treeDataO.push({ id: 'content_title_clc', label: 'CLC number' }); // } // 处理摘要图片 if (article.abstractImage) { var abstractImgStrBig = '<img ' + 'original-src=' + $('#v4_art_abstract_img img').attr('src'); $('#content_img_abstract').html('<li>' + article.abstractImage.replace('<img', abstractImgStrBig) + '</li>'); var abstractImgStr = '<img id="v4-art-abstract-img" onclick="showImageBtn(event, \'v4-art-abstract-img\')" ' + 'original-src=' + $('#v4_art_abstract_img img').attr('src'); $('#v4_art_abstract_img').html(article.abstractImage.replace('<img', abstractImgStr)); var dom = '<div class="v4-art-image">\n' + ' <span onclick="showImageBtn(event, \'v4-art-abstract-img\')">View original image</span>\n' + ' <span onclick="downloadImageBtn(event, \'v4-art-abstract-img\')">Download original image</span>\n' + ' </div>'; $('#v4_art_abstract_img').parent().append(dom); setTimeout(() => { // 图片大图模态初始化 if (document.getElementById('content_img_abstract')) { preViewImage = new Viewer(document.getElementById('content_img_abstract'), { url: 'original-src', }); } }, 10); } // 如果显示正文内容加载正文，不显示正文时，拼接显示增强出版资源、参考文献 if (ifShowFullText && article.strType !== 'Issue Information') { // 加载文章内容 loadFullTextInfo(); } else { // esm if (esmSize > 0) { treeDataO.push({ id: 'content_title_-11', label: 'Electronic Supplementary Material', labelWord: 'Electronic Supplementary Material', }); } // 参考文献 if (referenceList && referenceList['length'] > 0) { treeDataO.push({ id: 'content_title_-12', label: 'References' }); } articleApp.treeData = treeDataO; articleApp.treeDataReady = true; if ( esmSize === 0 && referenceList['length'] === 0 && !article.clcNumber && !article.documentsCode && !article.articleCodeId && !article.abstraction && !article.abstractToc && !article.abstractImage && !article['keypointsAbstracted'] && !article.erratum && articleKeyword === 0 ) { $('#article_no_data').css('display', 'block'); } } // 初始化图片表格轮播 function initSwiper() { // 初始化正文表格模态显示 galleryTopTable = new Swiper('.gallery-top-table', { nextButton: '.swiper-button-next-table', prevButton: '.swiper-button-prev-table', spaceBetween: 10, }); galleryThumbsTable = new Swiper('.gallery-thumbs-table', { spaceBetween: 10, centeredSlides: true, slidesPerView: 'auto', touchRatio: 0.2, slideToClickedSlide: true, }); galleryTopTable.params.control = galleryThumbsTable; galleryThumbsTable.params.control = galleryTopTable; } // 加载Altmetrics 是否存在 function isAltmetrics() { $.ajax({ // url: contextPath + '/article/altmetric?doi=' + doi, url: 'https://api.altmetric.com/v1/doi/' + doi, type: 'get', dataType: 'json', contentType: 'application/json', success: function(data) { if (data.score > 0) { $('#article_altmetric').css('display', 'block'); } }, error: function(err) { // alert(err.toString()); console.log(err); }, }); } setTimeout(function() { isAltmetrics(); }, 1000); // 点击图片显示大图 function showImage(event, dom) { if ($(dom).attr('class') !== 'inline-graphic') { event.stopPropagation(); var id = $(dom).attr('id'); var index = 0; for (var i = 0; i < imgShowListInfo.length; i++) { if (imgShowListInfo[i]['key'] === id) { index = i; $($('#content_img').children()[index]).find('img').click(); } } } } function showImageBtn(event, id) { event.stopPropagation(); if (id === 'v4-art-abstract-img') { $($('#content_img_abstract').children()[0]).find('img').click(); } else { var index = 0; for (var i = 0; i < imgShowListInfo.length; i++) { if (imgShowListInfo[i]['key'] === id) { index = i; $($('#content_img').children()[index]).find('img').click(); } } } } // 下载原图 function downloadImageBtn(event, id) { event.stopPropagation(); $.ajax({ url: contextPath + '/article/img/download?path=' + $('#' + id).attr('src'), type: 'get', dataType: 'json', contentType: 'application/json', success: function(data) { if ($(window).width() < 800) { window.location.href = data.object; } else { window.open(data.object); } }, error: function(err) { alert(err.toString()); }, }); } // 点击表格模态显示表格 function showTable(event) { return; // 如果是移动端去掉表格模态查看 => 屏幕小于600 去掉表格查看 if ($(window).width() < 600) { return; } var id = $(event).attr('id'); var index = 0; for (var i = 0; i < tableListInfo.length; i++) { if (tableListInfo[i]['key'] === id) { index = i; galleryTopTable.slideTo(index, 100, false); galleryThumbsTable.slideTo(index, 100, false); setTimeout(function() { $('.article-html-table-modal').css('margin-left', 0); }, 200); } } } function showTableBtn(event) { var id = $($(event).prev().children()[0]).attr('id'); var index = 0; for (var i = 0; i < tableListInfo.length; i++) { if (tableListInfo[i]['key'] === id) { index = i; galleryTopTable.slideTo(index, 100, false); galleryThumbsTable.slideTo(index, 100, false); setTimeout(function() { $('.article-html-table-modal').css('margin-left', 0); }, 200); } } } // 关闭模态 function closeModal() { $('.article-html-table-modal').css('margin-left', '-100%'); } // 新增轮询检测用户停留时间 $(document).ready(function() { setInterval(function() { $.ajax({ url: '/article/statistical_duration?id=' + articleId + '&uuid=' + uuid, type: 'get', }); }, 5000); }); // 统计下载量 function countDownLoadNum(id, ifPreview) { $.ajax({ url: contextPath + '/article/stat?articleId=' + id + '&ifPreview=' + ifPreview, type: 'get', dataType: 'json', contentType: 'application/json', }); } var user = null; function send_email(authorId, articleId) { if (user != null) { location.href = contextPath + '/user/user/article/send_email?authorId=' + authorId + '&articleId=' + articleId; } else { showYxAlert('warning', 'You are not currently logged in, please log in and operate!'); var code = window.location.pathname; var param = window.location.search; location.href = '/user/user/login_forward?tag=5&code=' + encodeURIComponent(code + param); } } // 点击xref标签跳转 function initTagClickHandler() { $('xref').click(function(event) { event = event || window.event; event.stopPropagation ? event.stopPropagation() : (event.cancelBubble = true); var refType = $(this).attr('ref-type'); var id = $(this).attr('rid'); switch (refType) { // 表格 case 'table': { var dom = $('#' + id).parent(); break; } // 图片 case 'fig': // 章节 case 'sec': // 公式 case 'disp-formula': { var dom = $('#' + id); break; } default: { } } jumpToId(dom); }); $('.html-table-content label').click(function() { var tableDom = $(this).closest('.html-table-content'); jumpToId(tableDom); }); $('fig label').click(function() { var figDom = $(this).closest('fig'); jumpToId(figDom); }); } function jumpToId(dom) { if (!dom) { return; } $(window).scrollTop(dom.offset().top - 100); dom.css('backgroundColor', '#eee'); setTimeout(function() { dom.css('backgroundColor', 'initial'); }, 1000); } // 正文节点滚动到可视范围内，选中展开对应的节点 $(window).scroll(function() { // 正文节点滚动到可视范围内，选中展开对应的节点 for (var d = 0; d < domArr.length; d++) { const domRect = domArr[d].getBoundingClientRect(); if (domRect.top < 150 && domRect.top > 0) { articleApp.setSelectedKey('content_' + $(domArr[d]).attr('id')); return; } if (domRect.bottom > 500 && domRect.top < 0) { articleApp.setSelectedKey('content_' + $(domArr[d]).attr('id')); return; } } }); window.addEventListener('scroll', function() { // 不显示正文的时候不统计浏览量 if (!ifShowFullText) { return } // 滚动到正文部分的时候掉接口 if (time > 1000 * 60 * 5 || num === 0) { if (document.getElementById('insert_content').getBoundingClientRect().top <= 150) { time = 0; num = num + 1; countDownLoadNum(articleId, ifPreview); } } }); var time = 0; var num = 0; var interval = setInterval(function() { time = time + 1000; }, 1000); window.addEventListener('beforeunload', (event) => { // 在这里执行你的清理工作 clearInterval(interval); }); </script> </html>

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Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus