Targeted Sequencing | GENEWIZ from Azenta

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interest. Whether you want to pinpoint a few genes or a few hundred, our custom targeted sequencing panels offer high sensitivity and specificity, providing in-depth coverage and high-quality data to discover point mutations, insertions/deletions (INDELs), copy number variations (CNVs), and gene rearrangements.</p> <p>To investigate the location and frequency of vector integration in the host genome following transduction, use our <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Services/Clinical-Services/CLIA-Integration-Site-Analysis" target="_blank">integration site analysis (ISA) solution</a> for pre-clinical and clinical cell and gene therapy research. </p> <p> </p> <p> </p> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://web.genewiz.com/ngs-inquiry?utm_source=targeted-reseq" target="_blank"><button>Request Quote</button></a> </div> <div class="ngs-content-box" id="ngs-content-box-right"> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://hubs.ly/Q02l4q0D0" target="_blank"><img alt="" src="/-/media/10013-D_Customer-Referral_WebBanRight1.ashx?la=en&hash=2F3AE4702AA3E83AD20D27ED70E249A396EBDECA" /></a> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .question { width: 1170px; display: inline-block; } .question p { width: 90%; text-align: left; } .question ul { width: 90%; text-align: left; } @media screen and (max-width: 1170px) { .question { width: auto; } } </style> <div class="question"> <h2>What is targeted sequencing?</h2> <p>Targeted sequencing is a technique that uses NGS to target specific genes, coding regions of the genome, or chromosomal segments for rapid identification and analysis of genetic mutations.</p> <p> </p> <h2>Why use targeted sequencing?</h2> <p>Targeted sequencing is useful for studying gene variants in selected genes or specific regions of the genome, since a large diversity of organisms is targeted rapidly and cost-effectively. Targeted sequencing is used to examine gene interactions in specific pathways and is faster and more cost effective than whole genome sequencing (WGS). This is because a smaller set of nucleotides are used, rather than broadly sequencing the entire genome.</p> <p> </p> </div> </div> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".SingleIcon").find("img").addClass("img-responsive center-block"); }); </script> <div class="process-v1 icon-nav Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center"> <h2>Targeted sequencing Gene Panels</h2> <p><p>GENEWIZ from Azenta provides a variety of targeted resequencing panels, either pre-made or custom, that can cover any genes of interest or cancer-implicated genes. </p> <p>Visit these pages to learn more about these services:</p></p> </div> <ul class="list-unstyled row "> <li class='col-sm-4'> <a href='/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels/cancer-panels' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/cancer-panels_110x210_Purple.ashx?h=110&la=en&w=210&hash=E537716E3E4F7BE24FA61EA53808F7F07944DF0C" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Cancer Panels</h3> <br> </div> <div class="row"> Including commercially available hotspot cancer panels, as well as Azenta’s pan-cancer and OncoGxOne™ discovery cancer panels.<br /> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='/Public/Services/Next-Generation-Sequencing/Targeted-Resequencing-Panels/Custom-Targeted-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/targeted-resequencing_110x210_Purple.ashx?h=110&la=en&w=210&hash=8989CF2EA77919B6CB23CDDF96B918523189B338" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>TargetGxOne™ (Custom Targeted Sequencing)</h3> <br> </div> <div class="row"> Our custom panels can be designed to target any genes of your choosing.<br> </div> </div> </a> </li>  <li class='col-sm-4'> <a href='/Public/Services/Next-Generation-Sequencing/Exome-Sequencing' target=''> <div class='SingleIcon headline-center Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="row"> <img src="/-/media/Images/Home/icons/Purple-Icons/exome-sequencing_110x210_Purple.ashx?h=110&la=en&w=210&hash=5739882864EC24919A7AE3FA7A7DADAC994A3029" alt="Building" width="210" height="110" /> </div> <div class="row"> <h3>Whole Exome Sequencing</h3> <br> </div> <div class="row"> A cost-effective alternative to whole genome sequencing, which allows genetic analysis of genomic coding regions. </div> </div> </a> </li>  </ul> <div class="row headline-center"> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <style type="text/css"> .body1 { text-align: center; display: inline-block; } .banner-background1 { width: 1170px; background-image: url('/-/media/13070-D_NGS-Platforms_WebBanFull-Lrg_Background.ashx'); background-size: cover; text-align: center; display: flex; padding: 10px; } .banner img1 { text-align: center; display: block; margin-left: auto; margin-right: auto; } #banner-left1 { width: 40%; padding: 30px; } #banner-right1 { width: 59%; padding: 30px; } #banner-right1 h2 { text-transform: none; letter-spacing: 1px; line-height: 40px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 40px; margin: 0; margin-bottom: 0px; color: #FFFFFF; } #banner-right1 h3 { text-transform: none; letter-spacing: 1px; line-height: 50px; border-bottom: none; font-family: 'Roboto', sans-serif; font-weight: 400; font-size: 20px; margin: 0; margin-bottom: 5px; color: #FFFFFF; } #banner-right1 button { padding: 8px 19px; color: 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height: auto; } #banner-right1 img { width: 90%; height: auto; } @media only screen and (max-width: 700px) { #banner-left1 img { width: 60%; } #banner-right1 button { font-size: 14px; } } </style> <div class="body1"> <div class="banner-background1"> <div id="banner-left1"> <img alt="" src="/-/media/13070-D_NGS-Platforms_WebBanFull-Lrg_Thumbnail.ashx?la=en&hash=2267956154B8A11D571A104DAF0356024C1845F7" /> </div> <div id="banner-right1"> <div id="banner-right1 h2"> <h3></h3> <h2>Take advantage of the latest long-read and short-read sequencing technology to accelerate your research</h2> <div id="banner-right1 h3"> <h3 style="color:#FFFFFF;"></h3> <br /> <a rel="noopener noreferrer" rel="noopener noreferrer" href="https://www.genewiz.com/en/Public/Resources/NGS-Platforms" target="_blank"><button>LEARN MORE</button></a> </div> </div> </div> </div> </div> </div> <script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Targeted Sequencing Applications</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Discover genetic biomarkers associated with disease state<br> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Characterize genetic associations to a particular phenotype, such as drug response<br> </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Genotype and classify eukaryotic and/or prokaryotic cell lines<br> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><i class="BlueCheck icon-custom icon-bg-light icon-4x fa fa-check"></i></div> <div class="content-boxes-in-v3"> Design genetic tests, such as for pharmacogenomics or oncology<br> </div> </div> </div> </div> </div> </div><script type="text/javascript" src="/assets/plugins/counter/jquery.counterup.min.js"></script> <script language="javascript" type="text/javascript"> jQuery(document).ready(function ($) { $(".row .category").find("img").addClass("img-responsive"); }); </script> <div class='Component-Frame pull-center Multi-column-TextBullet-Layout Component-BackGround-Default margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10'> <div class="container"> <div class="headline-center"> <h2>Targeted Sequencing Features & Benefits</h2> </div> <div class="headline-left"> </div> <div class="row category"> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> Extensive multiplexing flexibility enables a more cost-effective assay<br /> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> Dedicated, on-going support from experienced Azenta scientists<br> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> <strong>In-house pipeline</strong> for optional Sanger-based <a href="https://web.genewiz.com/confirm-your-ngs-data ">confirmatory sequencing</a> of final targets utilizing your NGS samples </div> </div> </div> <div class="col-md-6 col-sm-6"> <div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> Comprehensive bioinformatics options for robust downstream analysis<br /> </div> </div><div class="content-boxes-v3 margin-bottom-20"> <div class="col-md-2"><em style="font-size: 50px;" class="BlueCheck icon-custom icon-bg-gray icon-4x fa fa-check"></em><br /></div> <div class="content-boxes-in-v3"> Availability of the most cutting-edge sequencing platforms<br /> </div> </div> </div> </div> </div> </div><div class="process-v1 Image-Slider Component-BackGround-Gray margin-top-0 margin-bottom-30 padding-top-10 padding-bottom-10"> <div class="container"> <div class="headline-center margin-bottom-20"> <div class="row"> <h2> Workflow for Targeted Resequencing Panels </h2> </div> <div class="row"> Extensive Quality Control is Performed at the Conclusion of Each Step of the Process<br /> </div> </div> <ul class="list-inline list-st row process-in headline-center"> <li style="text-align:center;cursor:pointer;" class="ImgSlili_1"> <div class="row headline-center"> <img src="/-/media/Images/Services/NGS/Workflows/custom-data-analysis-deliverables_110x210-2.ashx?h=110&la=en&w=210&hash=61D5504AE9203DBD544CA5C4333B8C118D50D573" alt="" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Experimental Design</h3> <br /> </div> <div class="SeqIcon SeqIcon_1"> <span class="SeqIcon_text">1</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_1"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_2"> <div class="row headline-center"> <img src="/-/media/Images/Services/NGS/Workflows/library-prep-workflow_110x210.ashx?h=110&la=en&w=210&hash=72F52244BFEA9979481F926CC83C560EBF45FE87" alt="" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Targeting and Library Preparation</h3> <br /> </div> <div class="SeqIcon SeqIcon_2"> <span class="SeqIcon_text">2</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_2"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_3"> <div class="row headline-center"> <img src="/-/media/Images/Services/NGS/Workflows/ngs-platforms-workflow_110x210.ashx?h=110&la=en&w=210&hash=27F9E928B371FC12AC849DA70E9730E9D92C323F" alt="Building" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Sequencing</h3> <br> </div> <div class="SeqIcon SeqIcon_3"> <span class="SeqIcon_text">3</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_3"> <img src="/-/media/Gwz/arrow.ashx?h=50&la=en&w=70&hash=E9EE43042F03F59D57F7E4676486569A4310720A" alt="arrow" width="70" height="50" /> </li><li style="text-align:center;cursor:pointer;" class="ImgSlili_4"> <div class="row headline-center"> <img src="/-/media/Images/Services/NGS/Workflows/bioinformatics-workflow_110x210.ashx?h=110&la=en&w=210&hash=7F9DB8582A3653A987CDCC7A32AC04C489F24DB2" alt="" width="210" height="110" /> </div> <div class="row headline-center"> <h3>Bioinformatics</h3> <br /> </div> <div class="SeqIcon SeqIcon_4"> <span class="SeqIcon_text">4</span> </div> </li> <li style="vertical-align:bottom;text-align:center;opacity:1" class="Img-arror Img_4"> </li> </ul> </div> <script language="javascript" type="text/javascript"> var i = 1; var iconCount = 0; var icons; var iconContent; var Flag; jQuery(document).ready(function ($) { icons = $(".SeqIcon"); iconContent = $(".icon-content"); //hide the content if (iconContent != null && jQuery(".content_1").length > 0) { iconContent.each(function (i) { if (i > 0) { $(this).hide(); } }); } //change the icon status if (icons != null) { iconCount = icons.length; $(icons[0]).addClass("SeqIcon_Sel"); Flag = setInterval(function () { if (i == iconCount) { i = 0; } SwcRCA(i); i++; }, 2000); } $("li[class^=ImgSlili]").bind("mousemove", function () { clearInterval(Flag); jQuery("div[class^='row icon-content content_']").hide(); jQuery(".Img-arror").find("img").attr("src", "/assets/img/gwz/arrow.png"); jQuery(icons).removeClass("SeqIcon_Sel"); jQuery(".content_" + $(this).attr("class").split("_")[1]).show(); jQuery(icons[$(this).attr("class").split("_")[1] - 1]).addClass("SeqIcon_Sel"); jQuery(".Img_" + $(this).attr("class").split("_")[1]).find("img").attr("src", "/assets/img/gwz/arrow_sel.png"); }); $("li[class^=ImgSlili]").bind("mouseleave", function () { i = 0; Flag = setInterval(function () { SwcRCA(i); i++; }, 2000); jQuery(icons[$(this).attr("class").split("_")[1] - 1]).removeClass("SeqIcon_Sel"); jQuery(".Img_" + $(this).attr("class").split("_")[1]).find("img").attr("src", "/assets/img/gwz/arrow.png"); }); }); function SwcRCA(i) { if (i == 0) { jQuery(icons[icons.length - 1]).removeClass("SeqIcon_Sel"); } else { jQuery(icons[i - 1]).removeClass("SeqIcon_Sel"); } jQuery("div[class^='row icon-content content_']").hide(); jQuery(icons[i]).addClass("SeqIcon_Sel"); jQuery(".content_" + (i + 1)).show(); //change the arrow color jQuery(".Img_" + (i)).find("img").attr("src", "/assets/img/gwz/arrow.png"); if (i != (icons.length - 1)) { jQuery(".Img_" + (i + 1)).find("img").attr("src", "/assets/img/gwz/arrow_sel.png"); } } </script> </div><div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <h2>Sample Submission Guidelines</h2> <p>Azenta accepts the following sample types for targeted resequencing projects:</p> <div class="center-block" style="width: 80%;"> <ul style="float: left;"> <li style="text-align: left;">Purified genomic DNA</li> <li style="text-align: left;">Frozen cell pellets (mammalian or bacterial)</li> <li style="text-align: left;">PCR amplicons</li> <li style="text-align: left;">Bacterial colonies</li> </ul> <ul style="float: right;"> <li style="text-align: left;">Formalin-fixed, paraffin-embedded (FFPE) thin sections (slides)</li> <li style="text-align: left;">Select tissues</li> <li style="text-align: left;">Blood</li> <li style="text-align: left;">Swab</li> </ul> </div> </div> <br> <br> </div> <div class="headline-center Rich-Text-Edit-Area"> <link 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style="width: 100%; background-color: #f1f0f0; padding: 0; margin: 0;margin-top: -20px;"> <div class="tech-resources-container"> <h2> Technical Resources  </h2> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/MG_qPCR_Blog_US_Web-Tech-Thumb_Blue.ashx?la=en&hash=2A4957D1C2685CDC902644346D94E592804711C4" /> </div> <div class="resource resource-right"> <h3>Blog | NGS, PCR, or Sanger Sequencing: An Assay Selection Guide</h3> <p>This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making. <br /> <a href="https://blog.genewiz.com/ngs-qpcr-or-sanger-sequencing-an-assay-selection-guide?hs_preview=oBDChQvP-61477530694&utm_campaign=2022-03 GEN MG-qPCR&utm_source=Targeted Sequencing&utm_medium=technical resources&utm_content=US" target="_blank"> <button>Read Blog</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/13062-D_EU-Customer-Webinar_TechResource-Thumb.ashx?la=en&hash=74618789C1F38480E447D08C9D580DFDB40EFE6C" /> </div> <div class="resource resource-right"> <h3>Webinar| Non-Classical Sources of Tumour-Specific Antigen in Checkpoint Inhibitor Response</h3> <p>Developing immunotherapies for cancer can be difficult due to the variation of immune response from patient to patient. In this webinar, Dr. Litchfield from UCL Cancer Institute presents his team’s exploratory research using a multiomics approach to better understand the diversity of immune response to cancer and highlights their findings of an alternative source of a tumour-specific antigen in checkpoint inhibitor (CPI) response. <br /> <a href="https://www.azenta.com/resources/non-classical-sources-tumour-specific-antigen-checkpoint-inhibitor-response" target="_blank"> <button>Watch Now</button> </a> </p> </div> </div> <div class="tech-resource"> <div class="resource resource-left"> <img alt="" src="/-/media/Images/13063-D_Twist-Webinar_TechResource-Thumb.ashx?la=en&hash=C69D7D9DE09E6DEAC0A28A37C25C97DE56311C51" /> </div> <div class="resource resource-right"> <h3>Webinar | Demystifying End-to-End DNA Sequencing Solutions</h3> <p>In this co-hosted webinar with Twist Bioscience, learn how researchers design custom panels to sequence deeply and efficiently into regions, genes, and organisms of interest, as well as gain a greater understanding on how targeted sequencing can be combined with the latest transcriptomics and proteomics tools to reveal even more insights. <br /> <a href="https://www.azenta.com/resources/demystifying-end-end-dna-sequencing-solutions" target="_blank"> <button>Watch Now </button> </a> </p> </div> </div> </div> </div> </div> <div class="headline-center Rich-Text-Edit-Area"> <div class="container"> <div class="row"> <h2>NGS PLATFORMS</h2> <p>For information on our NGS platforms as well as recommended configurations of your projects, please visit the <a href="https://www.genewiz.com/en/Public/Resources/NGS-Platforms">NGS Platforms</a> page. <em>Azenta does not guarantee data output or quality for sequencing-only projects</em>.</p> </div> <div class="row row-centered margin-top-30"> <div class="col-sm-3 col-centered"><a href="#Illumina"><img alt="Illumina" src="/-/media/hires-illumina-logo-rgb_new.ashx?w=200&la=en&hash=2DE7778E5877DA9F3D063A9FAF021DA575ECF223" style="width: 200px;" /></a> </div> <div class="col-sm-3 col-centered"><a href="#PacBio"><img alt="PacBio" src="/-/media/PacBio-CSP_logo.ashx?w=200&la=en&hash=1568DC13B370C032463997ED7BC5591C1FECF672" style="width: 200px;" /></a> </div> <div class="col-sm-3 col-centered"><a href="#10x"><img alt="10x Genomics" src="/-/media/10x-Genomics-CSP_logo.ashx?w=200&la=en&hash=2584354CF0810D46E980E04C8C03461D332EE516" style="width: 200px;" /></a> </div> <div class="col-sm-3 col-centered"><a href="#Nanostring"><img alt="NanoString" src="/-/media/Images/Resources/NGS-Platforms-and-Specifications/NanoString_resized_logo.ashx?w=200&la=en&hash=A22CDFCF6A16A9884C633E8C2EF98B468325D130" style="width: 200px;" /></a> </div> </div> <div class="row row-centered margin-top-30"> <div class="col-sm-3 col-centered"><a href="#Oxford-Nanopore"><img alt="Nanopore" src="/-/media/ONT_ServiceCertified_logo-cut.ashx?w=200&la=en&hash=4140BDC9974099FF45B0BBB29FCC746FEDCFBCC7" style="width: 200px;" /></a> </div> <div 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