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Search results for: Loulwah Bokhari
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text-center" style="font-size:1.6rem;">Search results for: Loulwah Bokhari</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">10</span> Alexa (Machine Learning) in Artificial Intelligence</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Loulwah%20Bokhari">Loulwah Bokhari</a>, <a href="https://publications.waset.org/abstracts/search?q=Jori%20Nazer"> Jori Nazer</a>, <a href="https://publications.waset.org/abstracts/search?q=Hala%20Sultan"> Hala Sultan</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Nowadays, artificial intelligence (AI) is used as a foundation for many activities in modern computing applications at home, in vehicles, and in businesses. Many modern machines are built to carry out a specific activity or purpose. This is where the Amazon Alexa application comes in, as it is used as a virtual assistant. The purpose of this paper is to explore the use of Amazon Alexa among people and how it has improved and made simple daily tasks easier for many people. We gave our participants several questions regarding Amazon Alexa and if they had recently used or heard of it, as well as the different tasks it provides and whether it successfully satisfied their needs. Overall, we found that participants who have recently used Alexa have found it to be helpful in their daily tasks. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=artificial%20intelligence" title="artificial intelligence">artificial intelligence</a>, <a href="https://publications.waset.org/abstracts/search?q=Echo%20system" title=" Echo system"> Echo system</a>, <a href="https://publications.waset.org/abstracts/search?q=machine%20learning" title=" machine learning"> machine learning</a>, <a href="https://publications.waset.org/abstracts/search?q=feature%20for%20feature%20match" title=" feature for feature match"> feature for feature match</a> </p> <a href="https://publications.waset.org/abstracts/151249/alexa-machine-learning-in-artificial-intelligence" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/151249.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">121</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">9</span> Software Architecture Optimization Using Swarm Intelligence Techniques</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Arslan%20Ellahi">Arslan Ellahi</a>, <a href="https://publications.waset.org/abstracts/search?q=Syed%20Amjad%20Hussain"> Syed Amjad Hussain</a>, <a href="https://publications.waset.org/abstracts/search?q=Fawaz%20Saleem%20Bokhari"> Fawaz Saleem Bokhari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Optimization of software architecture can be done with respect to a quality attributes (QA). In this paper, there is an analysis of multiple research papers from different dimensions that have been used to classify those attributes. We have proposed a technique of swarm intelligence Meta heuristic ant colony optimization algorithm as a contribution to solve this critical optimization problem of software architecture. We have ranked quality attributes and run our algorithm on every QA, and then we will rank those on the basis of accuracy. At the end, we have selected the most accurate quality attributes. Ant colony algorithm is an effective algorithm and will perform best in optimizing the QA’s and ranking them. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=complexity" title="complexity">complexity</a>, <a href="https://publications.waset.org/abstracts/search?q=rapid%20evolution" title=" rapid evolution"> rapid evolution</a>, <a href="https://publications.waset.org/abstracts/search?q=swarm%20intelligence" title=" swarm intelligence"> swarm intelligence</a>, <a href="https://publications.waset.org/abstracts/search?q=dimensions" title=" dimensions"> dimensions</a> </p> <a href="https://publications.waset.org/abstracts/94992/software-architecture-optimization-using-swarm-intelligence-techniques" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/94992.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">261</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">8</span> Electrochemical Performance of Carbon Nanotube Based Supercapacitor </h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Jafar%20Khan%20Kasi">Jafar Khan Kasi</a>, <a href="https://publications.waset.org/abstracts/search?q=Ajab%20Khan%20Kasi"> Ajab Khan Kasi</a>, <a href="https://publications.waset.org/abstracts/search?q=Muzamil%20Bokhari"> Muzamil Bokhari </a> </p> <p class="card-text"><strong>Abstract:</strong></p> Carbon nanotube is one of the most attractive materials for the potential applications of nanotechnology due to its excellent mechanical, thermal, electrical and optical properties. In this paper we report a supercapacitor made of nickel foil electrodes, coated with multiwall carbon nanotubes (MWCNTs) thin film using electrophoretic deposition (EPD) method. Chemical vapor deposition method was used for the growth of MWCNTs and ethanol was used as a hydrocarbon source. High graphitic multiwall carbon nanotube was found at 750 C analyzing by Raman spectroscopy. We observed the electrochemical performance of supercapacitor by cyclic voltammetry. The electrodes of supercapacitor fabricated from MWCNTs exhibit considerably small equivalent series resistance (ESR), and a high specific power density. Electrophoretic deposition is an easy method in fabricating MWCNT electrodes for high performance supercapacitor. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=carbon%20nanotube" title="carbon nanotube">carbon nanotube</a>, <a href="https://publications.waset.org/abstracts/search?q=chemical%20vapor%20deposition" title=" chemical vapor deposition"> chemical vapor deposition</a>, <a href="https://publications.waset.org/abstracts/search?q=catalyst" title=" catalyst"> catalyst</a>, <a href="https://publications.waset.org/abstracts/search?q=charge" title=" charge"> charge</a>, <a href="https://publications.waset.org/abstracts/search?q=cyclic%20voltammetry" title=" cyclic voltammetry "> cyclic voltammetry </a> </p> <a href="https://publications.waset.org/abstracts/20355/electrochemical-performance-of-carbon-nanotube-based-supercapacitor" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/20355.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">563</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">7</span> SPR Immunosensor for the Detection of Staphylococcus aureus</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Muhammad%20Ali%20Syed">Muhammad Ali Syed</a>, <a href="https://publications.waset.org/abstracts/search?q=Arshad%20Saleem%20Bhatti"> Arshad Saleem Bhatti</a>, <a href="https://publications.waset.org/abstracts/search?q=Chen-zhong%20Li"> Chen-zhong Li</a>, <a href="https://publications.waset.org/abstracts/search?q=Habib%20Ali%20Bokhari"> Habib Ali Bokhari</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Surface plasmon resonance (SPR) biosensors have emerged as a promising technique for bioanalysis as well as microbial detection and identification. Real time, sensitive, cost effective, and label free detection of biomolecules from complex samples is required for early and accurate diagnosis of infectious diseases. Like many other types of optical techniques, SPR biosensors may also be successfully utilized for microbial detection for accurate, point of care, and rapid results. In the present study, we have utilized a commercially available automated SPR biosensor of BI company to study the microbial detection form water samples spiked with different concentration of Staphylococcus aureus bacterial cells. The gold thin film sensor surface was functionalized to react with proteins such as protein G, which was used for directed immobilization of monoclonal antibodies against Staphylococcus aureus. The results of our work reveal that this immunosensor can be used to detect very small number of bacterial cells with higher sensitivity and specificity. In our case 10^3 cells/ml of water have been successfully detected. Therefore, it may be concluded that this technique has a strong potential to be used in microbial detection and identification. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=surface%20plasmon%20resonance%20%28SPR%29" title="surface plasmon resonance (SPR)">surface plasmon resonance (SPR)</a>, <a href="https://publications.waset.org/abstracts/search?q=Staphylococcus%20aureus" title=" Staphylococcus aureus"> Staphylococcus aureus</a>, <a href="https://publications.waset.org/abstracts/search?q=biosensors" title=" biosensors"> biosensors</a>, <a href="https://publications.waset.org/abstracts/search?q=microbial%20detection" title=" microbial detection "> microbial detection </a> </p> <a href="https://publications.waset.org/abstracts/12570/spr-immunosensor-for-the-detection-of-staphylococcus-aureus" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/12570.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">475</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">6</span> Comparative Acaricidal Efficacy of Fluralaner vs Oral Ivermectin Against Tick Infestation in Dogs</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Tayyaba%20Zahra">Tayyaba Zahra</a>, <a href="https://publications.waset.org/abstracts/search?q=Shehla%20Gul%20Bokhari"> Shehla Gul Bokhari</a>, <a href="https://publications.waset.org/abstracts/search?q=Asim%20Khalid%20Mahmood"> Asim Khalid Mahmood</a>, <a href="https://publications.waset.org/abstracts/search?q=Raheela%20Akhtar"> Raheela Akhtar</a>, <a href="https://publications.waset.org/abstracts/search?q=Khizar%20Matloob"> Khizar Matloob</a> </p> <p class="card-text"><strong>Abstract:</strong></p> In Pakistan, dogs are commonly infested with ticks, especially in summers, causing not only dermatological issues but also systemic problems. Persistence of tick infestation often leads to heavy losses. Different acaricides are locally available with variable efficacy; however, recurrence of infestation is commonly reported. The present study was thus designed to compare the efficacy of a novel drug Fluralaner and conventionally used Ivermectin against tick infestation. Dogs positive for tick infestation were randomly divided into 2 groups viz, Groups A and B having 8 dogs each. Ticks were enumerated manually from the whole body of dogs at day 0 before the administration of drugs Dogs in Group A were treated with Fluralaner at day 0, and dogs in Group B were treated with Ivermectin. Post-treatment, ticks were counted again at days 7, 14, 21, 28, and 35. At day 07 of the study, no tick was found on the dogs treated with Fluralaner, while many ticks were present on the dogs treated with Ivermectin showing an efficacy up to 50%. On the consecutive follow-up evaluations, similar results were found for Fluralaner while the efficacy of Ivermectin was further reduced to less than 50%. Furthermore, Fluralaner treated dogs had better RBC counts, PCV, Hgb concentration, LFTs, RFTs post-treatment than the dogs treated with Ivermectin. Statistically, oral Fluralaner proved a more effective drug (P≤0.05)than oral Ivermectin against tick infestation in dogs. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=fluralaner" title="fluralaner">fluralaner</a>, <a href="https://publications.waset.org/abstracts/search?q=ivermectin" title=" ivermectin"> ivermectin</a>, <a href="https://publications.waset.org/abstracts/search?q=dogs" title=" dogs"> dogs</a>, <a href="https://publications.waset.org/abstracts/search?q=tick%20infestations" title=" tick infestations"> tick infestations</a> </p> <a href="https://publications.waset.org/abstracts/142770/comparative-acaricidal-efficacy-of-fluralaner-vs-oral-ivermectin-against-tick-infestation-in-dogs" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/142770.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">198</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">5</span> Investigations on the Cytotoxicity and Antimicrobial Activities of Terezine E and 14-Hydroxyterezine D</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Mariam%20Mojally">Mariam Mojally</a>, <a href="https://publications.waset.org/abstracts/search?q=Randa%20Abdou"> Randa Abdou</a>, <a href="https://publications.waset.org/abstracts/search?q=Wisal%20Bokhari"> Wisal Bokhari</a>, <a href="https://publications.waset.org/abstracts/search?q=Sultan%20Sab"> Sultan Sab</a>, <a href="https://publications.waset.org/abstracts/search?q=Mohammed%20Dawoud"> Mohammed Dawoud</a>, <a href="https://publications.waset.org/abstracts/search?q=Amjad%20Albohy"> Amjad Albohy</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Secondary metabolites produced by endophytes are an excellent source of biologically active compounds. In our current study, we evaluated terezine E and 14-hydroxyterezine D for binding to the active site of histone deacetylase (PDB ID: 4CBT) and matrix metalloproteinase 9 (PDB ID: 4H3X) by molecular docking using AutoDock Vina software after having tested their cytotoxic activities on three cell lines (human ductal breast epithelial tumor cells (T47D)-HCC1937), human hepatocarcinoma cell line (HepG2)-HB8065), and human colorectal carcinoma cells (HCT-116)-TCP1006, purchased from ATCC, USA)). Additionally, their antimicrobial activities were investigated, and their minimum inhibitory concentration (MIC) values were determined against P. notatum and S. aureus by the broth microdilution method. Higher cytotoxicity was observed for terezine E against all tested cell lines compared to 14-hydroxyterezine D. Molecular docking results supported the high cytotoxicity of terezine E and showed higher binding affinity with 4CBT with an energy score of 9 kcal/mol. Terezine E showed higher antibacterial and antifungal activities than 14-hydroxyrerezine D: MIC values were 15.45 and 21.73 mg/mL against S. aureus and 8.61 and 11.54 mg/mL against P. notatum, respectively <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Terezine%20E" title="Terezine E">Terezine E</a>, <a href="https://publications.waset.org/abstracts/search?q=14-Hydroxyterezine%20D" title=" 14-Hydroxyterezine D"> 14-Hydroxyterezine D</a>, <a href="https://publications.waset.org/abstracts/search?q=cytotoxicity" title=" cytotoxicity"> cytotoxicity</a>, <a href="https://publications.waset.org/abstracts/search?q=antimicrobial%20activity" title=" antimicrobial activity"> antimicrobial activity</a>, <a href="https://publications.waset.org/abstracts/search?q=molecular%20docking" title=" molecular docking"> molecular docking</a> </p> <a href="https://publications.waset.org/abstracts/179873/investigations-on-the-cytotoxicity-and-antimicrobial-activities-of-terezine-e-and-14-hydroxyterezine-d" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/179873.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">73</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">4</span> Risk Factors for Fall in Elderly with Diabetes Mellitus Type 2 in Jeddah Saudi Arabia 2022: A Cross-Sectional Study</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Rami%20S.%20Alasmari">Rami S. Alasmari</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdullah%20Al%20Zahrani"> Abdullah Al Zahrani</a>, <a href="https://publications.waset.org/abstracts/search?q=Hattan%20A.%20Hassani"> Hattan A. Hassani</a>, <a href="https://publications.waset.org/abstracts/search?q=Hattan%20A.%20Hassani"> Hattan A. Hassani</a>, <a href="https://publications.waset.org/abstracts/search?q=Nawwaf%20A.%20Almalky"> Nawwaf A. Almalky</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdullah%20F.%20Bokhari"> Abdullah F. Bokhari</a>, <a href="https://publications.waset.org/abstracts/search?q=Alwalied%20A.%20Hafez"> Alwalied A. Hafez</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Diabetes mellitus type 2 (DMT2) is a major chronic condition that is considered common among elderly people, with multiple potential complications that could contribute to falls. However, this concept is not well understood, thus, the aim of this study is to determine whether diabetes is an independent risk factor for falls in elderly. In this observational cross-sectional study, 309 diabetic patients aged 60 or more who visited the primary healthcare centers of the Ministry of National Guard Health Affairs in Jeddah were chosen via convenience sampling method. To collect the data, Semi-structured Fall Risk Assessment questionnaire and Fall Efficacy Score scale were used. The mean age of the participants was estimated to be 68.5 (SD:7.4) years. Among the participants, 48.2% experienced falling before, and 63.1% of them suffered falls in the past 12-months. The results showed that gait problems were independently associated with a higher likelihood of fall among the elderly patients (OR = 1.98, 95%CI, 1.08 to 3.62, p = 0.026. This paper suggests that diabetes mellitus is an independent fall risk factor among elderly. Therefore, identifying such patients as being at higher risk and prompt referral to a specialist falls clinic is recommended. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diabetes" title="diabetes">diabetes</a>, <a href="https://publications.waset.org/abstracts/search?q=fall" title=" fall"> fall</a>, <a href="https://publications.waset.org/abstracts/search?q=elderly" title=" elderly"> elderly</a>, <a href="https://publications.waset.org/abstracts/search?q=risk%20factors" title=" risk factors"> risk factors</a> </p> <a href="https://publications.waset.org/abstracts/159194/risk-factors-for-fall-in-elderly-with-diabetes-mellitus-type-2-in-jeddah-saudi-arabia-2022-a-cross-sectional-study" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/159194.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">105</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">3</span> Effect of Supplemental Bacterial Phytase at Different Dietary Levels of Phosphorus on Tibial Bone Characteristics and Body Weight Gain in Broilers</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Saqib%20Saleem%20Abdullah">Saqib Saleem Abdullah</a>, <a href="https://publications.waset.org/abstracts/search?q=Saima%20Masood"> Saima Masood</a>, <a href="https://publications.waset.org/abstracts/search?q=Hafsa%20Zaneb"> Hafsa Zaneb</a>, <a href="https://publications.waset.org/abstracts/search?q=Shela%20Gul%20Bokhari"> Shela Gul Bokhari</a>, <a href="https://publications.waset.org/abstracts/search?q=Muti%20Ur%20Rehman"> Muti Ur Rehman</a>, <a href="https://publications.waset.org/abstracts/search?q=Jamil%20Akbar"> Jamil Akbar</a> </p> <p class="card-text"><strong>Abstract:</strong></p> A 5- weeks feeding trial was carried out to determine the effectiveness of Bacterial Phytase (Phyzyme®) in broilers, at different dietary levels of Phosphorous. 140 d-old broilers (Hubbard) were randomly divided into 4 groups (n=4). Birds were fed corn-based basal diet or the same diet supplemented with 3 different levels of non Phytate Phosphorous (NPP) (0.45 %, 0.30 % and 0.15 %). Furthermore, the diets were supplemented with bacterial Phytase. Birds were fed ad libitum and kept under thermo neutral conditions. The parameters studied were; body weight gain (BWG), tibial bone characteristics (TBC), serum Calcium (Ca), Phosphorus (P) and Alkaline Phosphatase (AP) levels and tibia ash percentage (TAP). BWG of the broilers was calculated at weekly interval and remaining parameters were calculated after slaughtering the birds at 35thday. Results suggested that Phytase supplementation at 0.30% NPP (Non Phytate Phosphorus + Bacterial Phytase) increased (P < 0.05) the BWG, bone length, bone weight, tibiotarsal index, medullary canal diameter and diaphysis diameter however, rubosticity index was reduced to minimum (P < 0.05) at this dietary level of phosphorous when compared with other groups. Maximum (P < 0.05) rubosticity index was observed in control group with 0% Phytase. Furthermore, Phytase addition at 0.30 % NPP also improved (P < 0.05) Ca, P and AP levels in the blood. Phytase supplementation at lower phosphorus level (0.30%NPP) improved BWG and TBC including bone density and bone quality in broilers hence it can be concluded that addition of Phytase at 0.30% NPP may prove beneficial for bone and overall performance in broilers. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=diaphysis%20diameter" title="diaphysis diameter">diaphysis diameter</a>, <a href="https://publications.waset.org/abstracts/search?q=phytase" title=" phytase"> phytase</a>, <a href="https://publications.waset.org/abstracts/search?q=rubosticity%20index" title=" rubosticity index"> rubosticity index</a>, <a href="https://publications.waset.org/abstracts/search?q=tibia" title=" tibia"> tibia</a> </p> <a href="https://publications.waset.org/abstracts/66841/effect-of-supplemental-bacterial-phytase-at-different-dietary-levels-of-phosphorus-on-tibial-bone-characteristics-and-body-weight-gain-in-broilers" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/66841.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">395</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">2</span> Molecular Characterization of Two Thermoplastic Biopolymer-Degrading Fungi Utilizing rRNA-Based Technology</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Nuha%20Mansour%20Alhazmi">Nuha Mansour Alhazmi</a>, <a href="https://publications.waset.org/abstracts/search?q=Magda%20Mohamed%20Aly"> Magda Mohamed Aly</a>, <a href="https://publications.waset.org/abstracts/search?q=Fardus%20M.%20Bokhari"> Fardus M. Bokhari</a>, <a href="https://publications.waset.org/abstracts/search?q=Ahmed%20Bahieldin"> Ahmed Bahieldin</a>, <a href="https://publications.waset.org/abstracts/search?q=Sherif%20Edris"> Sherif Edris</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Out of 30 fungal isolates, 2 new isolates were proven to degrade poly-β-hydroxybutyrate (PHB). Enzyme assay for these isolates indicated the optimal environmental conditions required for depolymerase enzyme to induce the highest level of biopolymer degradation. The two isolates were basically characterized at the morphological level as Trichoderma asperellum (isolate S1), and Aspergillus fumigates (isolate S2) using standard approaches. The aim of the present study was to characterize these two isolates at the molecular level based on the highly diverged rRNA gene(s). Within this gene, two domains of the ribosome large subunit (LSU) namely internal transcribed spacer (ITS) and 26S were utilized in the analysis. The first domain comprises the ITS1/5.8S/ITS2 regions ( > 500 bp), while the second domain comprises the D1/D2/D3 regions ( > 1200 bp). Sanger sequencing was conducted at Macrogen (Inc.) for the two isolates using primers ITS1/ITS4 for the first domain, while primers LROR/LR7 for the second domain. Sizes of the first domain ranged between 594-602 bp for S1 isolate and 581-594 bp for S2 isolate, while those of the second domain ranged between 1228-1238 bp for S1 isolate and 1156-1291 for S2 isolate. BLAST analysis indicated 99% identities of the first domain of S1 isolate with T. asperellum isolates XP22 (ID: KX664456.1), CTCCSJ-G-HB40564 (ID: KY750349.1), CTCCSJ-F-ZY40590 (ID: KY750362.1) and TV (ID: KU341015.1). BLAST of the first domain of S2 isolate indicated 100% identities with A. fumigatus isolate YNCA0338 (ID: KP068684.1) and strain MEF-Cr-6 (ID: KU597198.1), while 99% identities with A. fumigatus isolate CCA101 (ID: KT877346.1) and strain CD1621 (ID: JX092088.1). Large numbers of other T. asperellum and A. fumigatus isolates and strains showed high level of identities with S1 and S2 isolates, respectively, based on the diversity of the first domain. BLAST of the second domain of S1 isolate indicated 99 and 100% identities with only two strains of T. asperellum namely TR 3 (ID: HM466685.1) and G (ID: KF723005.1), respectively. However, other T. species (ex., atroviride, hamatum, deliquescens, harzianum, etc.) also showed high level of identities. BLAST of the second domain of S2 isolate indicated 100% identities with A. fumigatus isolate YNCA0338 (ID: KP068684.1) and strain MEF-Cr-6 (ID: KU597198.1), while 99% identities with A. fumigatus isolate CCA101 (ID: KT877346.1) and strain CD1621 (ID: JX092088.1). Large numbers of other A. fumigatus isolates and strains showed high level of identities with S2 isolate. Overall, the results of molecular characterization based on rRNA diversity for the two isolates of T. asperellum and A. fumigatus matched those obtained by morphological characterization. In addition, ITS domain proved to be more sensitive than 26S domain in diversity profiling of fungi at the species level. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=Aspergillus%20fumigates" title="Aspergillus fumigates">Aspergillus fumigates</a>, <a href="https://publications.waset.org/abstracts/search?q=Trichoderma%20asperellum" title=" Trichoderma asperellum"> Trichoderma asperellum</a>, <a href="https://publications.waset.org/abstracts/search?q=PHB" title=" PHB"> PHB</a>, <a href="https://publications.waset.org/abstracts/search?q=degradation" title=" degradation"> degradation</a>, <a href="https://publications.waset.org/abstracts/search?q=BLAST" title=" BLAST"> BLAST</a>, <a href="https://publications.waset.org/abstracts/search?q=ITS" title=" ITS"> ITS</a>, <a href="https://publications.waset.org/abstracts/search?q=26S" title=" 26S"> 26S</a>, <a href="https://publications.waset.org/abstracts/search?q=rRNA" title=" rRNA"> rRNA</a> </p> <a href="https://publications.waset.org/abstracts/87955/molecular-characterization-of-two-thermoplastic-biopolymer-degrading-fungi-utilizing-rrna-based-technology" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/87955.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">159</span> </span> </div> </div> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Medical Dressing Induced Digital Ischemia in Patient with Congenital Insensitivity to Pain and Anhidrosis</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=Abdulwhab%20Alotaibi">Abdulwhab Alotaibi</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdullah%20Alzahrani"> Abdullah Alzahrani</a>, <a href="https://publications.waset.org/abstracts/search?q=Ziyad%20Bokhari"> Ziyad Bokhari</a>, <a href="https://publications.waset.org/abstracts/search?q=Abdulelah%20Alghamdi"> Abdulelah Alghamdi</a> </p> <p class="card-text"><strong>Abstract:</strong></p> First described in 1975 by Dr. Miller, Medical dressings are uncommon but possible cause of hand digital ischemia due the tourniquet-like effect. The incident of this complication has been reported across wide range of age-groups, yet it seems like that the pediatric population are specifically vulnerable. Multiple dressing types were reported to have caused ischemic injury, such as elastic wrap, tubular gauze, and self-adherent dressings. We present a case of medical dressing induced digital ischemia in patient with Congenital insensitivity to pain and anhidrosis (CIPA), which further challenge the discovery of the condition. An 8-year-old girl known case of CIPA. Brought by her mother to the ER after nail bed injury, which she managed by application of elastic wrap that was left for 24 hours. When the mother found out she immediately removed the elastic band, and noticed the fingertip was black and cold with tense bullae. The color then changed later when she arrived to the ER to dark purple with bluish discoloration on the tip. On examination there was well demarcated tense bullae on the distal right fifth finger. Neurovascular intact, pulse oximetry on distal digit 100%, capillary refill time was delayed. She was seen under Plastic surgery and conservative management recommended, and patient was discharged with safety netting. Two days later the patient came as follow-up visit at which her condition demonstrated significant improvement, the bullae has since ruptured leaving behind sloughed skin, capillary refill and pulse oximetry were both within normal limits, sensory function couldn’t be assessed but her motor function and ROM were normal, topical bacitracin and bandage dressings were applied for the eroded skin. Patient was scheduled for a follow-up in 2 weeks. Preventatively it’s advisable to avoid the commonly implicated dressings such as elastic, tubular gauze or self-adherent wraps in hand or digital injuries when possible, but in cases where the use of these dressings is of necessity the appropriate precautions must be taken, Dr. Makarewich proposed the following 5 measures to help minimize the incidence of the injury: 1-Unwrapping 12 inches of the dressing before rolling the injured finger. 2-Wrapping from distal to proximal with minimal tension to avoid vascular embarrassment. 3-The use of 5-25 inch to overlap the entire wrap. 4-Maintaining light pressure over the wrap to allow adherence of the dressing. 5-Minimization of the number of layers used to wrap the affected digit. Also assessing the capillary refill after the application can help in determining the patency of the supplying blood vessels. It’s also important to selectively determine if the patient is a candidate for conservative management, as tailored approach can help in maximizing the positive outcomes for our patients. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=congenital%20insensitivity%20to%20pain" title="congenital insensitivity to pain">congenital insensitivity to pain</a>, <a href="https://publications.waset.org/abstracts/search?q=digital%20ischemia" title=" digital ischemia"> digital ischemia</a>, <a href="https://publications.waset.org/abstracts/search?q=medical%20dressing" title=" medical dressing"> medical dressing</a>, <a href="https://publications.waset.org/abstracts/search?q=conservative%20management" title=" conservative management"> conservative management</a> </p> <a href="https://publications.waset.org/abstracts/171931/medical-dressing-induced-digital-ischemia-in-patient-with-congenital-insensitivity-to-pain-and-anhidrosis" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/171931.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">63</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div class="col-md-2"> 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