Search results for: M. Hoelker

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Hoelker</title> <meta name="description" content="Search results for: M. Hoelker"> <meta name="keywords" content="M. Hoelker"> <meta name="viewport" content="width=device-width, initial-scale=1, minimum-scale=1, maximum-scale=1, user-scalable=no"> <meta charset="utf-8"> <link href="https://cdn.waset.org/favicon.ico" type="image/x-icon" rel="shortcut icon"> <link href="https://cdn.waset.org/static/plugins/bootstrap-4.2.1/css/bootstrap.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/plugins/fontawesome/css/all.min.css" rel="stylesheet"> <link href="https://cdn.waset.org/static/css/site.css?v=150220211555" rel="stylesheet"> </head> <body> <header> <div class="container"> <nav class="navbar navbar-expand-lg navbar-light"> <a class="navbar-brand" href="https://waset.org"> <img src="https://cdn.waset.org/static/images/wasetc.png" alt="Open Science Research Excellence" title="Open Science Research Excellence" /> </a> <button class="d-block d-lg-none navbar-toggler ml-auto" type="button" data-toggle="collapse" data-target="#navbarMenu" aria-controls="navbarMenu" aria-expanded="false" aria-label="Toggle navigation"> <span class="navbar-toggler-icon"></span> </button> <div class="w-100"> <div class="d-none d-lg-flex flex-row-reverse"> <form method="get" action="https://waset.org/search" class="form-inline my-2 my-lg-0"> <input class="form-control mr-sm-2" type="search" placeholder="Search Conferences" value="M. 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Hoelker"> <input type="submit" class="btn_search" value="Search"> </div> </div> </form> </div> </div> <div class="row mt-3"> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Commenced</strong> in January 2007</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Frequency:</strong> Monthly</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Edition:</strong> International</div> </div> </div> <div class="col-sm-3"> <div class="card"> <div class="card-body"><strong>Paper Count:</strong> 1</div> </div> </div> </div> <h1 class="mt-3 mb-3 text-center" style="font-size:1.6rem;">Search results for: M. Hoelker</h1> <div class="card paper-listing mb-3 mt-3"> <h5 class="card-header" style="font-size:.9rem"><span class="badge badge-info">1</span> Let-7 Mirnas Regulate Inflammatory Cytokine Production in Bovine Endometrial Cells after Lipopolysaccharide Challenge by Targeting TNF伪</h5> <div class="card-body"> <p class="card-text"><strong>Authors:</strong> <a href="https://publications.waset.org/abstracts/search?q=S.%20Ibrahim">S. Ibrahim</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Salilew-Wondim"> D. Salilew-Wondim</a>, <a href="https://publications.waset.org/abstracts/search?q=M.%20Hoelker"> M. Hoelker</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Looft"> C. Looft</a>, <a href="https://publications.waset.org/abstracts/search?q=E.%20Tholen"> E. Tholen</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Grosse-Brinkhaus"> C. Grosse-Brinkhaus</a>, <a href="https://publications.waset.org/abstracts/search?q=K.%20Schellander"> K. Schellander</a>, <a href="https://publications.waset.org/abstracts/search?q=C.%20Neuhoff"> C. Neuhoff</a>, <a href="https://publications.waset.org/abstracts/search?q=D.%20Tesfaye"> D. Tesfaye</a> </p> <p class="card-text"><strong>Abstract:</strong></p> Bovine endometrial cells appear to have a key role in innate immune defense of the female genital tract. A better understanding of molecular changes in microRNAs (miRNAs) and their target genes expression may identify reliable prognostic indicators for cows that will resolve inflammation and resume cyclicity. In the current study, we hypothesized that let-7 miRNAs family has a primary role in the innate immune defence of the endometrium tissue against bacterial infection, which is partly achieved via regulating mRNA stability of pro-inflammatory cytokines at the post-transcriptional level. Therefore, we conducted two experiments. In the first experiment, primary bovine endometrial cells were challenged with clinical (3.0 渭g/ml) and sub-clinical (0.5 渭g/ml) doses of lipopolysaccharide (LPS) for 24h. In the 2nd experiment, we have investigated the potential role of let-7 miRNAs (let-7a and let-7f) using gain and loss of function approaches. Additionally, tumor necrosis factor alpha (TNF伪), transforming growth factor beta 1 induced transcript 1 (TGFB1I1) and serum deprivation response (SDPR) genes were validated using reporter assay. Here we addressed for the first time that let-7 miRNAs have a precise role in bovine endometrium, where LPS dysregulated let-7 miRNAs family expression was associated with an increased pro-inflammatory cytokine level by directly/indirectly targeting the TNF伪, interleukin 6 (IL6), nuclear factor kappa-light-chain enhancer of activated B cells (NF魏B), TGF尾1I1 and SDPR genes. To our knowledge, this is the first study showing that TNF伪, TGF尾1I1 and SDPR were identified and validated as novel let-7 miRNAs targets and could have a distinct role in inflammatory immune response of LPS challenged bovine endometrial cells. Our data represent a new finding by which uterine homeostasis is maintained through functional regulation of let-7a by down-regulation of pro-inflammatory cytokines expression (TNF伪 and IL6) at the mRNA and protein levels. These findings suggest that LPS serves as a negative regulator of let-7 miRNAs expression and provides a mechanism for the persistent pro-inflammatory phenotype, which is a hallmark of bovine subclinical endometritis. <p class="card-text"><strong>Keywords:</strong> <a href="https://publications.waset.org/abstracts/search?q=bovine%20endometrial%20cells" title="bovine endometrial cells">bovine endometrial cells</a>, <a href="https://publications.waset.org/abstracts/search?q=let-7" title=" let-7"> let-7</a>, <a href="https://publications.waset.org/abstracts/search?q=lipopolysaccharide" title=" lipopolysaccharide"> lipopolysaccharide</a>, <a href="https://publications.waset.org/abstracts/search?q=pro-inflammatory%20cytokines" title=" pro-inflammatory cytokines"> pro-inflammatory cytokines</a> </p> <a href="https://publications.waset.org/abstracts/38494/let-7-mirnas-regulate-inflammatory-cytokine-production-in-bovine-endometrial-cells-after-lipopolysaccharide-challenge-by-targeting-tnfa" class="btn btn-primary btn-sm">Procedia</a> <a href="https://publications.waset.org/abstracts/38494.pdf" target="_blank" class="btn btn-primary btn-sm">PDF</a> <span class="bg-info text-light px-1 py-1 float-right rounded"> Downloads <span class="badge badge-light">380</span> </span> </div> </div> </div> </main> <footer> <div id="infolinks" class="pt-3 pb-2"> <div class="container"> <div style="background-color:#f5f5f5;" class="p-3"> <div class="row"> <div class="col-md-2"> <ul class="list-unstyled"> About <li><a href="https://waset.org/page/support">About Us</a></li> <li><a href="https://waset.org/page/support#legal-information">Legal</a></li> <li><a target="_blank" rel="nofollow" href="https://publications.waset.org/static/files/WASET-16th-foundational-anniversary.pdf">WASET celebrates its 16th foundational anniversary</a></li> </ul> </div> <div class="col-md-2"> <ul class="list-unstyled"> Account <li><a href="https://waset.org/profile">My Account</a></li> </ul> </div> <div 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Search results for: M. Hoelker