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'' : value; }, getCookie: function (key) { var result = ""; var cookies = document.cookie ? document.cookie.split('; ') : []; for (var i = 0; i < cookies.length; i++) { var parts = cookies[i].split('='); var name = decodeURIComponent(parts.shift()); var cookie = parts ? parts.join('=') : []; // Prevent storing or parsing of a cookie that we couldn't decode. if (cookie && key === name) { // removes surrounding quotes if (cookie.indexOf('"') === 0) { // This is a quoted cookie as according to RFC2068, unescape... cookie = cookie.slice(1, -1).replace(/\\"/g, '"').replace(/\\\\/g, '\\'); } // parse utf8 characters try { cookie = decodeURIComponent(cookie); } catch (e) {} result = cookie; // we found it, don't bother looking at other cookies break; } } return result; }, setCookie : function(name, value, numDays){ if ( !numDays ) numDays = 31; var expires = new Date(); expires.setTime(expires.getTime() + (1000 * 60 * 60 * 24 * numDays)); document.cookie = name+ '=' + escape(value) + '; path=/; expires=' + expires.toGMTString() + '; domain=.' + this.getMasterDomain() + ';'; }, /** * get the master domain based on business rules */ getMasterDomain : function () { // default the master domain var masterDomain = 'thermofisher.com'; // check for alternate domain portion of URL var regexMatch = document.location.hostname.match(/\.(thermofisher)\.(cn)/i); if (regexMatch !== null) { masterDomain = regexMatch[1] + '.' + regexMatch[2]; } return masterDomain; }, /** * Check if init has been initiated once * @type {Boolean} */ initted: false, /** * @return {Array} method returns an array of a given object's key properties */ init: function () { this.page.country = this.getCookie("CK_ISO_CODE") || 'us'; this.page.language = this.getCookie('CK_LANG_CODE') || 'en'; this.page.siteName = 'thermo'; //hard coded this.initMerchandisingData(); this.initUserData(); this.displayDigitalData(); this.formSubmitEvent(); }, //specific function to set type of the page // need specific function to keep page type separate from other page attributes setPageType: function (type) { this.setPageAttribute('type', type); }, // generic function to set page attributes setPageAttribute: function (key, value) { this.page[key] = value; }, // display digitalData object displayDigitalData: function () { }, // specific function to add an object to digitalData addDigitDataObject: function (key, object) { this[key] = object; }, addChildObjectAttribute: function(child, key, value){ this[child][key] = value; }, initUserData: function(){ var gigyaCookie = this.getCookie("identity_uid"); this.user.gigyaId = ""; this.user.loginStatus = "anonymous"; if( gigyaCookie != ""){ this.user.gigyaId = gigyaCookie; this.user.loginStatus = "logged in"; } }, /** * Check if init has been initiated once * @type {Boolean} */ merchandisingDataInitted: false, initMerchandisingData: function () { // read cid and icid value from session cookie this.merchandising.icid = this.getCookie('s_ev21_tc'); this.merchandising.cid = this.getCookie('s_cmp_tc'); // EMID value this.merchandising.emid = this.getQueryParam("emid"); // referrer by domain if (typeof document.referrer !== 'undefined' && document.referrer.length > 0) { var masterDomain = this.getMasterDomain(); 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document.dispatchEvent(customClickEvent); } } } })(); window.digitalData.init(); </script>  <script type="text/javascript"> window.performance && window.performance.mark && window.performance.mark("header_25"); </script> <script type="text/javascript"> var localizedStrings = { "ACCEPTANCE": "Acceptance of Terms of Use", "BTN_AGREE": "Agree", "BTN_DISAGREE": "Disagree", "PROCESSING_MESSAGE" :"processing ... please wait ...", "ADD_TO_CART_QUANTITY_ERROR":"You must provide a quantity to add an item to the cart", "ADD_TO_CART_VALID_QUANTITY_ERROR":"You must provide a valid quantity to add an item to the cart", "VIEW_CART" :"View cart & checkout", "VIEW_CONTACT_DETAIL" :"View Contact Details", "ADD_TO_CART_NOTIFICATION":"Add to Cart Notification", "UPDATE_CART" :"Update Cart", "PRINCIPAL_INVESTIGATOR":"Principal Investigator", "PRINCIPAL_INVESTIGATOR_TEAM_LEADER":"Principal Investigator or Team Leader", "PRINCIPAL_INVESTIGATOR_CHIEF":"Principal Investigator or Chief", "EMAIL_ERROR_1" :"Please Provide Collegues Email", "EMAIL_ERROR_2" :"Please enter a valid colleague email address", "EMAIL_ERROR_3" :"Please Provide Your Email", "EMAIL_ERROR_4" :"Please enter a valid email address", "ERROR_OCCURED_MESSAGE" :"The following errors occured", "NEXT" :"Next", "PREVIOUS" :"Prev", "IMAGE" :"Image", "SAVE_TO_LIST_ERROR_1" :"Please enter a list name.", "SAVE_TO_LIST_ERROR_2" :"Please enter a different list name that does not include the following: ?,/,\\,%,^,!", "MINICART_ERROR_1" :"Unable to connect to eCommerce system. 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src="/content/dam/LifeTech/Thermo-Scientific/CMD/Banners/swoosh-background-ms-1600x390.jpg" alt="" height="390px" width="1600px" class="well-background-image"/><div class="well-content"><div class="parsys"><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><div class="hero-banner-content"><h1>What is mass spectrometry?</h1><p><a href="#menu5"><span class="btn btn-featured">Contact us</span></a>  <a href="/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/mass-spectrometry-resource-library/lc-ms-resources.html"><span class="btn btn-primary">Resource library</span></a></p></div></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"></div></div><div style="clear:both"></div></div></div></div></div></div></div></div></div></div><div class="container page-header-hidden"><div class="row"><div class="span12"><div class="pageheading"></div></div></div></div><div class="container-wrap template-1-column"><div class="container"><div class="row"><div class="span12 main" role="main"><div class="MainParsys parsys"><div class="text parbase section"><div class="breakout nav-shadow"><div class="container"><div class="row-fluid gds-nav sticky"><div class="span3 gds-nav-item"><a href="#menu1" class="gds-nav-link"><span class="gds-nav-text" id="jump-links-nav1">Overview</span></a></div><div class="span2 gds-nav-item"><a href="#menu2" class="gds-nav-link"><span class="gds-nav-text" id="jump-links-nav2">Ionization</span></a></div><div class="span3 gds-nav-item"><a href="#menu3" class="gds-nav-link"><span class="gds-nav-text" id="jump-links-nav3">Mass analyzers</span></a></div><div class="span3 gds-nav-item"><a href="#menu4" class="gds-nav-link"><span class="gds-nav-text" id="jump-links-nav4">Molecular dissociation</span></a></div><div class="span3 gds-nav-item"><a href="#menu5" class="gds-nav-link"><span class="gds-nav-text" id="jump-links-nav5">Contact us</span></a></div></div></div></div></div><div class="text parbase section"><h2 style="text-align: center;" id="menu1"> <br>  <br>  <br> What is mass spectrometry?</h2></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt7"><div class="parsys_column cq-colctrl-lt7-c0"><div container-id="cq-colctrl-lt7" comp-id="A1"><div class="text parbase section"><p>Mass spectrometry (MS) involves four basic steps: ionization, ion separation by a mass analyzer, detection, and data processing and analysis. In modern mass spectrometers, these steps are managed by computer software. Following ionization, analytes are transmitted into the vacuum chamber of the mass spectrometer. Here, the mass analyzer separates the ions based on their mass-to-charge ratios (<i>m/z</i>). The The detector generates a signal representative of ion abundance at specific values of <i>m/z</i>, and this signal is processed and recorded by computer software by a detector and the resulting signal is measured and recorded by computer software.</p><p>Using certain MS technologies, tandem MS can be performed by isolating and imparting precursor ions with internal energy to cause dissociation, resulting in fragment ions which are then measured as a MS/MS fragment ion spectrum. Tandem MS is often abbreviated as MS/MS or MS2.  Additionally, specific MS/MS product ions can be isolated and dissociated into a higher order product ion spectrum (referred to as MS3). This process can be repeated to obtain additional levels of MS (referred to as MS<sup>n</sup>).  When combined with chromatography, such as liquid, ion, or gas chromatography, MS and MS<sup>n</sup> are powerful analytical tools for compound identification and quantitation.</p></div></div></div><div class="parsys_column cq-colctrl-lt7-c1"><div container-id="cq-colctrl-lt7" comp-id="B1"><div class="well-cmp well-default well-blue-light section"><div class="well well-blue-light well-default"><div class="well-container" style=""><div class="well-content"><div class="parsys"><div class="heading section"><h3> Topics in mass spectrometry </h3></div><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/gas-chromatography-mass-spectrometry-gc-ms-information.html" title="Gas Chromatography Mass Spectrometry (GC-MS) Information">Gas Chromatography Mass Spectrometry (GC-MS) Information</a></li><li><a href="/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/liquid-chromatography-mass-spectrometry-lc-ms-information.html" title="Liquid Chromatography Mass Spectrometry (LC-MS) Information">Liquid Chromatography Mass Spectrometry (LC-MS) Information</a></li><li><a href="/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/mass-spectrometry-applications-area.html" title="Mass Spectrometry Applications Areas">Mass Spectrometry Applications Areas</a></li><li><a href="/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/mass-spectrometry-technology-overview.html" title="Mass Spectrometry Technology Overview">Mass Spectrometry Technology Overview</a></li></ul></div><div class="heading section"><h3> Related resources </h3></div><div class="text parbase section"><ul class="the-icons clearfix"><li><a href="/sg/en/home/life-science/protein-biology/protein-mass-spectrometry-analysis/mass-spectrometry-analysis-technical-handbooks.html">Mass Spec Analysis Technical Handbooks</a></li><li><a href="/sg/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-mass-spectrometry.html">Overview of Protein Mass Spectrometry</a></li><li><span class="icon-lt-technical-documentation icon-lt-blue"></span><a href="/sg/en/home/technical-resources.html">Technical support</a></li><li><span class="icon-lt-cart icon-lt-blue"></span><a href="/sg/en/home/industrial/mass-spectrometry.html">Learn about products</a></li><li><span class="icon-lt-resources icon-lt-blue"></span><a href="/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/mass-spectrometry-resource-library.html">Mass Spectrometry Resource Library</a></li></ul></div></div></div></div></div></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><h2 id="menu2" style="text-align: center;"> <br>  <br>  <br> Ionization</h2></div><div class="text parbase section"><p>Atoms and molecules must be ionized before they can be separated in the mass analyzer and detected by their mass-to-charge ratio (<i>m/z</i>). Ionization methods include <a href="/order/catalog/product/IQLAAEGABBFAGOMZZZ">atmospheric pressure chemical ionization (APCI)</a>, atmospheric pressure photo ionization (APPI), electron ionization (EI), <a href="/order/catalog/product/IQLAAEGABBFACTMAJI">electrospray ionization (ESI)</a>, chemical ionization (CI), inductively coupled plasma (ICP) ionization, and matrix-assisted laser desorption/ionization (MALDI). Different ionization methods are used depending on the sample characteristics and experimental goals.</p></div><div class="text parbase section"><h2 id="menu3" style="text-align: center;"> <br>  <br>  <br> Types of mass analyzers</h2></div><div class="text parbase section"><p>Examples of mass analyzers include single <a href="/sg/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/single-quadrupole-lc-ms.html">quadrupole</a>, <a href="/sg/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/triple-quadrupole-lc-ms.html">triple quadrupole</a>, <a href="/sg/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/single-quadrupole-lc-ms.html">quadrupole ion trap</a>, orbital electrostatic ion trap (e.g., the Thermo Scientific Orbitrap mass analyzer), Fourier transform ion cyclotron resonance (FT-ICR), magnetic sector, and time-of-flight (TOF). Different mass analyzers excel at different types of experiments. For example, quadrupoles are often used in quantitative studies, while ion traps are chosen for qualitative and structural work. Mass analyzers can also be combined to create tandem, <a href="/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/single-quadrupole-lc-ms.html">hybrid</a> or Thermo Scientific <a href="/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/single-quadrupole-lc-ms.html">Tribrid</a> mass spectrometers that perform advanced experiments or improve experimental results and throughput.</p></div><div class="accordion section"><div class="accordion" id="14eb984d726e4860989cb7e98c3fc879" style='margin-bottom: 0;'><div class="parsys"><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#14eb984d726e4860989cb7e98c3fc879" href="#massquad"><span class="caret"></span> What is a quadrupole mass analyzer? </a></div><div class="accordion-body collapse " id="massquad"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>A quadrupole mass analyzer uses DC and RF voltages applied to four parallel rod electrodes to create electrical fields that selectively stabilize or destabilize the path of particular ions though the mass filter based on their <i>m/z</i>.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#14eb984d726e4860989cb7e98c3fc879" href="#triple"><span class="caret"></span> What is a triple quadrupole mass analyzer? </a></div><div class="accordion-body collapse " id="triple"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>When a linear series of three quadrupoles is used, the resulting triple stage quadrupole analyzer is able to both filter and fragment the ion stream. In a typical mode of operation, the first (Q1) and third (Q3) quadrupoles act as mass filters, while the second (Q2) quadrupole dissociates ions by collision with a neutral gas such as argon, helium or nitrogen.</p><p>In this configuration, triple quadrupole MS systems can be operated to perform Selected Reaction Monitoring (SRM). SRM is a highly selective data acquisition mode where the set of DC and RF voltages applied to Q1 selectively transmit a narrow <i>m/z </i>range centered on the target precursor <i>m/z</i> value. Selected precursor ions exit Q1 into Q2 where the precursor ions collide with the neutral gas molecules, producing a series of product ions in the process known as collision-induced dissociation (CID). The product ions exit Q2 into Q3, where the narrow <i>m/z</i> ranges associated with a specific fragment ion are filtered for subsequent detection. The dwell time is the amount of time the triple quadrupole mass spectrometer measures ion intensity for this SRM transition and is typically around 10 milliseconds.</p><p>For targeted screening and quantitation, performing tandem MS using SRM significantly improves selectivity, sensitivity and, provided the product ions monitored are diagnostic of the target compound, the specificity needed for accurate and robust targeted quantitation. The additional selectivity obtained reduces the need for extensive sample preparation, increasing experimental productivity.</p><p>The level of selectivity offered by triple quadrupole systems is valuable for the analysis of target compounds in the fields of drug testing, food safety, environmental analysis, and clinical and forensic toxicology studies.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#14eb984d726e4860989cb7e98c3fc879" href="#iontrap"><span class="caret"></span> What is a quadrupole ion trap mass analyzer? </a></div><div class="accordion-body collapse " id="iontrap"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>Historically, most quadrupole ion traps used in mass spectrometry are based on the model invented by Wolfgang Paul (who shared in the 1989 Nobel Prize). Known as a three-dimensional ion trap, the Paul-based ion trap uses frequency (RF) electrical fields to trap ions for analysis. These Ion traps utilize three electrodes with hyperbolic surfaces function to trap ions: two endcap electrodes and a central ring electrode. Ions may be scanned out of the trap in order of their <i>m/z</i>'s by varying the amplitude of the trapping voltage over time, which causes ions of a particular <i>m/z</i> to develop unstable trajectories or to be resonantly ejected via use of a supplemental oscillatory field. </p><p>Quadrupole ion traps may alternatively be constructed with a two-dimensional geometry. Such traps, often referred to as "linear" ion traps, employ four rod electrodes arranged in parallel, and confine ions to an elongated central channel through the application of RF voltages to the rod electrodes (which provides confinement in the radial dimensions) and the application of DC voltages to end lenses or outer segments of the rod electrodes (which provides confinement in the axial dimension).</p><p>The ion trap’s stability offers high sensitivity and enables the selection and study of individual ion/molecule reactions through MS<sup>n</sup>. As a result, ion traps are well-suited for the discovery and targeted analysis of molecules and molecular reactions in fields such as metabolomics and lipidomics, as well as post-translational modification analysis.</p></div></div></div></div></div></div></div><div class="accordionitem section"><div class='accordion-group' ><div class='accordion-heading '><a class="accordion-toggle" data-toggle="collapse" data-parent="#14eb984d726e4860989cb7e98c3fc879" href="#orbitrap"><span class="caret"></span> What is an Orbitrap mass analyzer? </a></div><div class="accordion-body collapse " id="orbitrap"><div class="accordion-inner"><div class="accordion-content"><div class="itemParsys parsys"><div class="text parbase section"><p>The Orbitrap mass analyzer is an orbital electrostatic ion trap based on a modified Kingdon ion trap design. It consists of a central spindle-like electrode surrounded by an outer barrel-shaped electrode.  The outer electrode is laterally split into two symmetrical halves. Electrostatic voltages are applied to either or both of the inner and outer electrodes, producing an electric field that causes ions injected into the annular space between the inner and outer electrodes to undergo orbital motion around the central electrode and harmonic "back and forth" motion along the dimension defined by the central axis. The frequency of harmonic motion is characteristic of the ions' <i>m/z</i>.</p><p>The back and forth motion of the trapped ions within the Orbitrap analyzer produces an image current on the split halves of the outer electrode, which is processed to determine the frequencies and abundances of the ons. The presence of hundreds or thousands of different ions of different <i>m/z</i> induces a very complex image current (sometimes referred to as a "transient"), which is resolved and converted into mass spectra using a mathematical process called Fourier transformation. The Orbitrap mass analyzer’s high-resolution capability is due to accurate measurement of frequencies at MHz rates combined with precise time measurements.</p><p>Orbitrap-based mass spectrometers are used for targeted and non-targeted analysis of a wide variety of applications that benefit from high-resolution/accurate mass analysis, including unknown compounds in areas of biomarker discovery, proteomics, metabolism, food safety and environmental testing, clinical research, and forensic toxicology.</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt4"><div class="parsys_column cq-colctrl-lt4-c0"><div container-id="cq-colctrl-lt4" comp-id="A1"><div class="image parbase section"><div id="cq-image-jsp-9c377a2d-dbb4-409b-9f12-e4baaf64060aMainParsysaccordion_9613itemsparaccordionitem_582citemParsysimage_b5f2"><a href="/order/catalog/product/BRE725539"><picture><source srcset='https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.320.low.jpg/1704312831236.jpg' media="(min-width: 1px)"><source srcset='https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.320.medium.jpg/1704312831236.jpg' media="(min-width: 320px)"><source srcset='https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.480.medium.jpg/1704312831236.jpg' media="(min-width: 490px)"><source srcset='https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.620.high.jpg/1704312831236.jpg' media="(min-width: 630px)"><source srcset='https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.full.high.jpg/1704312831236.jpg' media="(min-width: 1025px)"><img srcset="https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.full.high.jpg/1704312831236.jpg,https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.320.high.jpg/1704312831236.jpg,https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.480.high.jpg/1704312831236.jpg,https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.320.medium.jpg/1704312831236.jpg,https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.620.high.jpg/1704312831236.jpg,https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.320.low.jpg/1704312831236.jpg,https://www.thermofisher.com/sg/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/jcr:content/MainParsys/accordion_9613/itemspar/accordionitem_582c/itemParsys/image_b5f2/foregroundimg.img.480.medium.jpg/1704312831236.jpg" alt='Orbitrap Exploris 480 Mass Spectrometer' title='Orbitrap Exploris 480 Mass Spectrometer'></picture><style> #cq-image-jsp-9c377a2d-dbb4-409b-9f12-e4baaf64060aMainParsysaccordion_9613itemsparaccordionitem_582citemParsysimage_b5f2 img{ width:100%; 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max-width: 1160px; } </style><script type="text/javascript"> $( document ).ready(function() { // apply fillIt plugin var fillClasses = "#cq-image-jsp-"+"9c377a2d-dbb4-409b-9f12-e4baaf64060aMainParsysaccordion_9613itemsparaccordionitem_582citemParsysimage_360 .image-contain" + "," + "#cq-image-jsp-"+"9c377a2d-dbb4-409b-9f12-e4baaf64060aMainParsysaccordion_9613itemsparaccordionitem_582citemParsysimage_360 .image-cover" ; // Due to the issue explained below, we are binding 'one' instead of 'on' and we need it only once. $(fillClasses).load(function() { $(this).fillIt(); }); // Force load image to avoid cache issue, a bug which makes image reset height when having more than one image/image-text components on the same page and rendering after edit. $(fillClasses).each(function(){ if(this.complete){ $(this).load(); } }); if(!!window.picturefill){ window.picturefill(); } }); </script></a></div></div></div><div container-id="cq-colctrl-lt4" comp-id="D2"><div class="text parbase section"><h4 style="text-align: center;"><a href="/sg/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-tribrid-mass-spectrometers/orbitrap-ascend-tribrid-mass-spectrometer.html">Orbitrap Ascend Tribrid Mass Spectrometer</a><br></h4></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p>Now a portfolio of three instruments designed to meet the needs of everyday research and analytical laboratories with next-level insights for scientific exploration and targeted quantitation. With performance and operational consistency, and market-leading resolving power, our next-generation, advanced high-resolution accurate-mass (HRAM) quadrupole-Orbitrap mass spectrometers deliver ease of use without sacrificing maximum performance.</p><p><a href="/sg/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms.html">Learn more ›</a></p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><h4>What is a TOF mass analyzer?</h4><p>A time-of-flight (TOF) mass analyzer separates ions of different masses based on their flight times over a known distance. Ions are created and then pulsed out with equal kinetic energy by an optical element into a flight tube. Because lighter ions travel faster than heavier ions of the same kinetic energy, the time of their arrival at the detector is used to determine <i>m/z</i>.</p><p>The quadrupole-TOF mass spectrometer combines a quadrupole mass filter (Q1) and a collision cell (Q2) with a TOF mass analyzer to produce MS/MS spectra. Q1 can be operated to transmit all ions or as a mass filter to select specific ions based on <i>m/z</i>. After leaving the collision cell, the ions are analyzed by TOF as described above.</p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><h4>What is a hybrid mass analyzer?</h4><p>The various mass analyzers can be combined in one mass spectrometer to perform advanced experiments with different separation, resolution, and detection goals. For example, the Thermo Scientific Orbitrap Exploris 480 mass spectrometer includes a quadrupole mass filter to better select precursor ions prior to their injection into and HRAM detection by an Orbitrap mass analyzer. The Orbitrap mass analyzer can resolve spectral peaks having almost the same <i>m/z</i> values. Furthermore, this instrument contains an HCD collision cell to effectively fragment ions for unambiguous confirmation of molecular identities.</p></div></div></div></div><div style="clear:both"></div><div class="text parbase section"><p>There are many other hybrid mass spectrometers that combine different mass analyzers, including the following:</p><ul><li>Q-TOF: quadrupole + time of flight (TOF)</li><li>Q-Trap: quadrupole + ion trap</li><li>Q Exactive mass spectrometer: quadrupole + Orbitrap mass analyzer<br></li></ul><p>These hybrid mass spectrometers combine the best features of each mass analyzer, providing additional analytical performance and versatility.</p><p>Tribrid mass spectrometers like the Thermo Scientific Orbitrap Eclipse mass spectrometer combine quadrupole, linear ion trap, and Orbitrap mass analyzers for MS, MS2, MS<sup>n</sup> analyses which offer more fragmentation capabilities for deeper analysis of samples</p></div></div></div></div></div></div></div></div></div><script> $j(document).ready(function(){ $j('#14eb984d726e4860989cb7e98c3fc879').on('hide', function(e) { $(e.target).find(".accordion-content").hide(); }); $j('#14eb984d726e4860989cb7e98c3fc879').on('show', function(e) { $(e.target).find(".accordion-content").show(); }); }); </script></div><div class="text parbase section"><h2 id="menu4" style="text-align: center;"> <br>  <br>  <br> Molecular dissociation</h2></div><div class="text parbase section"><p>To better understand the structural characteristics of a molecule, a dissociation technique may be used to break it up into fragments prior to MS analysis. The additional fragment ion peaks provide a molecular "fingerprint" that can help a scientist to determine its molecular structure.  In diagnostics and applied laboratories, several fragmentation stages may be used to confirm the identity of a compound. Learn more about molecular dissociation techniques.</p></div><div comp-order="bycol" class="parsys_column cq-colctrl-lt0"><div class="parsys_column cq-colctrl-lt0-c0"><div container-id="cq-colctrl-lt0" comp-id="A1"><div class="text parbase section"><h4>Detection</h4><p>After m/z-based analyzer in the mass filter, ions can be detected using a variety of detectors including electron multipliers, scintillators, conversion dynodes, or Faraday cups. In these cases, the detectors multiply the signal produced by the incident ions by producing elections and photons.</p><p>In addition, Orbitrap and certain other mass analyzers, ion detection is performed using an image current induced on the electrodes of the mass analyzer by ion motion. The complex image current is resolved and converted into mass spectra using Fourier transformation. This approach takes multiplexed measurements rather than direct measurement to provide an improvement in resolution and in signal to noise ratio (known as Fellgett's advantage).</p></div></div></div><div class="parsys_column cq-colctrl-lt0-c1"><div container-id="cq-colctrl-lt0" comp-id="B1"><div class="text parbase section"><h4>Data processing and analysis</h4><p>In addition to providing control of complex MS instrument and method parameters, computing software is used to perform data processing and to produce useful results from the vast amounts of raw MS data generated. There are numerous software tools available. 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container-id="cq-colctrl-lt3" comp-id="A1"><div class="customtext section"><script type="text/javascript" src="/etc/designs/komodo/clientlibs/form.min.699c139eae6c5da24b611b0bcb433e33.js"></script><div class="form_row clearfix"><div class="form_leftcol"><div class="form_leftcollabel"><label for="SID_Eloqua_Form_C_EmailAddress">Email</label></div><div class="form_leftcolmark"> *</div></div><div class="form_rightcol " id="C_EmailAddress_rightcol"><div id="C_EmailAddress_0_wrapper" class="form_rightcol_wrapper"><input maxlength="" onclick="errorCheck('true', 'FormIntent')" class="form_field form_field_text" id="SID_Eloqua_Form_C_EmailAddress" name="C_EmailAddress" value="" size="35"></div></div></div></div></div><div container-id="cq-colctrl-lt3" comp-id="A2"><div class="customtext section"><div class="form_row clearfix"><div class="form_leftcol"><div class="form_leftcollabel"><label for="SID_Eloqua_Form_C_FirstName">First Name</label></div><div class="form_leftcolmark"> *</div></div><div class="form_rightcol " id="C_FirstName_rightcol"><div id="C_FirstName_0_wrapper" class="form_rightcol_wrapper"><input maxlength="" onclick="errorCheck('true', 'FormIntent')" class="form_field form_field_text" id="SID_Eloqua_Form_C_FirstName" name="C_FirstName" value="" size="35"></div></div></div></div></div><div container-id="cq-colctrl-lt3" comp-id="A3"><div class="customtext section"><div class="form_row clearfix"><div class="form_leftcol"><div class="form_leftcollabel"><label for="SID_Eloqua_Form_C_LastName">Last Name</label></div><div class="form_leftcolmark"> *</div></div><div class="form_rightcol " id="C_LastName_rightcol"><div id="C_LastName_0_wrapper" class="form_rightcol_wrapper"><input maxlength="" onclick="errorCheck('true', 'FormIntent')" class="form_field form_field_text" id="SID_Eloqua_Form_C_LastName" name="C_LastName" value="" size="35"></div></div></div></div></div><div container-id="cq-colctrl-lt3" comp-id="A4"><div class="customtext section"><div class="form_row 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id="SID_Eloqua_Form_C_BusPhone" name="C_BusPhone" value="" size="35"></div></div></div></div></div></div><div class="parsys_column cq-colctrl-lt3-c1"><div container-id="cq-colctrl-lt3" comp-id="B1"><div class="customtext section"><div class="form_row clearfix"><div class="form_leftcol"><div class="form_leftcollabel"><label for="SID_Eloqua_Form_C_Address1">Street Address</label></div><div class="form_leftcolmark"> *</div></div><div class="form_rightcol " id="C_Address1_rightcol"><div id="C_Address1_0_wrapper" class="form_rightcol_wrapper"><input maxlength="" onclick="errorCheck('true', 'FormIntent')" class="form_field form_field_text" id="SID_Eloqua_Form_C_Address1" name="C_Address1" value="" size="35"></div></div></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B2"><div class="dropdown section"><script type="text/javascript" src="/apps/foundation/components/form/dropdown/widgets.min.6726155f9a47032731f55d62c7614758.js"></script><div class="form_row clearfix"><div class="form_leftcol"><div class="form_leftcollabel"><label for="SID_Eloqua_Form_C_Country">Country/Region</label></div><div class="form_leftcolmark"> *</div></div><div class="form_rightcol " id="C_Country_rightcol"><select class="form_field form_field_select" id="SID_Eloqua_Form_C_Country" name="C_Country" ><option disabled selected value="- Select -">- Select -</option><option value="US">United States</option><option value="GB">United Kingdom</option><option value="CA">Canada</option><option value="IN">India</option><option value="NL">Netherlands</option><option value="AU">Australia</option><option value="ZA">South Africa</option><option value="FR">France</option><option value="DE">Germany</option><option value="SG">Singapore</option><option value="SE">Sweden</option><option value="BR">Brazil</option><option value="blank">----------------</option><option value="AF">Afghanistan</option><option value="AX">Åland Islands</option><option value="AL">Albania</option><option value="DZ">Algeria</option><option value="AS">American Somoa</option><option value="AD">Andorra</option><option value="AO">Angola</option><option value="AI">Anguilla</option><option value="AQ">Antartica</option><option value="AG">Antigua and Barbuda</option><option value="AR">Argentina</option><option value="AM">Armenia</option><option value="AW">Aruba</option><option value="AT">Austria</option><option value="AZ">Azerbaijan</option><option value="BS">Bahamas</option><option value="BH">Bahrain</option><option value="BD">Bangladesh</option><option value="BB">Barbados</option><option value="BY">Belarus</option><option value="BE">Belgium</option><option value="BZ">Belize</option><option value="BJ">Benin</option><option value="BM">Bermuda</option><option value="BT">Bhutan</option><option value="BO">Bolivia</option><option value="BA">Bosnia and Herzegovina</option><option value="BW">Botswana</option><option value="BV">Bouvet Island</option><option value="IO">Brit/Indian Ocean Terr.</option><option value="BN">Brunei Darussalam</option><option value="BG">Bulgaria</option><option value="BF">Burkina Faso</option><option value="BI">Burundi</option><option value="KH">Cambodia</option><option value="CM">Cameroon</option><option value="CV">Cape Verde</option><option value="KY">Cayman Islands</option><option value="CF">Central African Republic</option><option value="TD">Chad</option><option value="CL">Chile</option><option value="CN">China</option><option value="CX">Christmas Island</option><option value="CC">Cocos (Keeling) Islands</option><option value="CO">Columbia</option><option value="KM">Comoros</option><option value="CG">Congo</option><option value="CD">Congo, The Dem. Republic Of</option><option value="CK">Cook Islands</option><option value="CR">Costa Rica</option><option value="CI">Côte D'Ivore</option><option value="HR">Croatia</option><option value="CU">Cuba</option><option value="CY">Cyprus</option><option value="CZ">Czech Republic</option><option value="DK">Denmark</option><option value="DJ">Djibouti</option><option value="DM">Dominica</option><option value="DO">Dominican Republic</option><option value="EC">Ecuador</option><option value="EG">Egypt</option><option value="SV">El Salvador</option><option value="GQ">Equatorial Guinea</option><option value="ER">Eritrea</option><option value="EE">Estonia</option><option value="ET">Ethiopia</option><option value="FK">Falkland Islands</option><option value="FO">Faroe Islands</option><option value="FJ">Fiji</option><option value="FI">Finland</option><option value="GF">French Guiana</option><option value="PF">French Polynesia</option><option value="TF">French Southern Terr.</option><option value="GA">Gabon</option><option value="GM">Gambia</option><option value="GE">Georgia</option><option value="GH">Ghana</option><option value="GI">Gibraltar</option><option value="GR">Greece</option><option value="GL">Greenland</option><option value="GD">Grenada</option><option value="GP">Guadeloupe</option><option value="GU">Guam</option><option value="GT">Guatemala</option><option value="GN">Guinea</option><option value="GW">Guinea-Bissau</option><option value="GY">Guyana</option><option value="HT">Haiti</option><option value="HM">Heard/McDonald Isls.</option><option value="HN">Honduras</option><option value="HK">Hong Kong</option><option value="HU">Hungary</option><option value="IS">Iceland</option><option value="ID">Indonesia</option><option value="IR">Iran</option><option value="IQ">Iraq</option><option value="IE">Ireland</option><option value="IL">Israel</option><option value="IT">Italy</option><option value="JM">Jamaica</option><option value="JP">Japan</option><option value="JO">Jordan</option><option value="KZ">Kazakhstan</option><option value="KE">Kenya</option><option value="KI">Kiribati</option><option value="KP">Korea (North)</option><option value="KR">Korea (South)</option><option value="KW">Kuwait</option><option value="KG">Kyrgyzstan</option><option value="LA">Laos</option><option value="LV">Latvia</option><option value="LB">Lebanon</option><option value="LS">Lesotho</option><option value="LR">Liberia</option><option value="LY">Libya</option><option value="LI">Liechtenstein</option><option value="LT">Lithuania</option><option value="LU">Luxembourg</option><option value="MO">Macau</option><option value="MK">Macedonia</option><option value="MG">Madagascar</option><option value="MW">Malawi</option><option value="MY">Malaysia</option><option value="MV">Maldives</option><option value="ML">Mali</option><option value="MT">Malta</option><option value="MH">Marshall Islands</option><option value="MQ">Martinique</option><option value="MR">Mauritania</option><option value="MU">Mauritius</option><option value="YT">Mayotte</option><option value="MX">Mexico</option><option value="FM">Micronesia</option><option value="MD">Moldova</option><option value="MC">Monaco</option><option value="MN">Mongolia</option><option value="MS">Montserrat</option><option value="MA">Morocco</option><option value="MZ">Mozambique</option><option value="MM">Myanmar</option><option value="MP">N. Mariana Isls.</option><option value="NA">Namibia</option><option value="NR">Nauru</option><option value="NP">Nepal</option><option value="AN">Netherlands Antilles</option><option value="NC">New Caledonia</option><option value="NZ">New Zealand</option><option value="NI">Nicarugua</option><option value="NE">Nigeria</option><option value="NU">Niue</option><option value="NF">Norfolk Island</option><option value="NO">Norway</option><option value="OM">Oman</option><option value="PK">Pakistan</option><option value="PW">Palau</option><option value="PS">Palestinian Territory, Occupied</option><option value="PA">Panama</option><option value="PG">Papua New Guinea</option><option value="PY">Paraguay</option><option value="PE">Peru</option><option value="PH">Philippines</option><option value="PN">Pitcairn</option><option value="PL">Poland</option><option value="PT">Portugal</option><option value="PR">Puerto Rico</option><option value="QA">Qatar</option><option value="RE">Reunion</option><option value="RO">Romania</option><option value="RU">Russian Federation</option><option value="RW">Rwanda</option><option value="KN">Saint Kitts and Nevis</option><option value="LC">Saint Lucia</option><option value="WS">Samoa</option><option value="SM">San Marino</option><option value="ST">Sao Tome/Principe</option><option value="SA">Saudi Arabia</option><option value="SN">Senegal</option><option value="CS">Serbia and Montenegro</option><option value="SC">Seychelles</option><option value="SL">Sierra Leone</option><option value="SK">Slovak Republic</option><option value="SI">Slovenia</option><option value="SB">Solomon Islands</option><option value="SO">Somalia</option><option value="ES">Spain</option><option value="LK">Sri Lanka</option><option value="SH">St. Helena</option><option value="PM">St. Pierre and Miquelon</option><option value="VC">St. Vincent and Grenadines</option><option value="SD">Sudan</option><option value="SR">Suriname</option><option value="SJ">Svalbard/Jana Mayen Isls.</option><option value="SZ">Swaziland</option><option value="CH">Switzerland</option><option value="SY">Syria</option><option value="TW">Taiwan</option><option value="TJ">Tajikistan</option><option value="TZ">Tanzania</option><option value="TH">Thailand</option><option value="TL">Timor-Leste</option><option value="TG">Togo</option><option value="TK">Tokelau</option><option value="TO">Tonga</option><option value="TT">Trinidad and Tobago</option><option value="TN">Tunisia</option><option value="TR">Turkey</option><option value="TM">Turkmenistan</option><option value="TC">Turks/Caicos Isls.</option><option value="TV">Tuvalu</option><option value="UG">Uganda</option><option value="UA">Ukraine</option><option value="AE">United Arab Emirates</option><option value="UM">United Minor Outlying Is.</option><option value="UY">Uruguay</option><option value="UZ">Uzbekistan</option><option value="VU">Vanuatu</option><option value="VA">Vatican City</option><option value="VE">Venezuela</option><option value="VN">Vietnam</option><option value="VG">Virgin Islands (British)</option><option value="VI">Virgin Islands (U.S.)</option><option value="WF">Wallis/Futuna Isls.</option><option value="EH">Western Sahara</option><option value="YE">Yemen</option><option value="ZM">Zambia</option><option value="ZW">Zimbabwe</option></select></div></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B3"><div class="dropdown section"><div class="form_row clearfix"><div class="form_leftcol"><div class="form_leftcollabel"><label for="SID_Eloqua_Form_C_State_Prov">State/Province</label></div><div class="form_leftcolmark"> *</div></div><div class="form_rightcol " id="C_State_Prov_rightcol"><select class="form_field form_field_select" id="SID_Eloqua_Form_C_State_Prov" name="C_State_Prov" ><option value="">- Select -</option><option value="AK">Alaska</option><option value="AL">Alabama</option><option value="AR">Arkansas</option><option value="AS">American Samoa</option><option value="AZ">Arizona</option><option value="CA">California</option><option value="CO">Colorado</option><option value="CT">Connecticut</option><option value="DC">D.C.</option><option value="DE">Delaware</option><option value="FL">Florida</option><option value="FM">Micronesia</option><option value="GA">Georgia</option><option value="GU">Guam</option><option value="Hi">Hawaii</option><option value="IA">Iowa</option><option value="ID">Idaho</option><option value="IL">Illinois</option><option value="IN">Indiana</option><option value="KS">Kansas</option><option value="KY">Kentucky</option><option value="LA">Louisiana</option><option value="MA">Massachusetts</option><option value="MD">Maryland</option><option value="ME">Maine</option><option value="MH">Marshall Islands</option><option value="MI">Michigan</option><option value="MN">Minnesota</option><option value="MO">Missouri</option><option value="MP">Marianas</option><option value="MS">Mississippi</option><option value="MT">Montana</option><option value="NC">North Carolina</option><option value="ND">North Dakota</option><option value="NE">Nebraska</option><option value="NH">New Hampshire</option><option value="NJ">New Jersey</option><option value="NM">New Mexico</option><option value="NV">Nevada</option><option value="NY">New York</option><option value="OH">Ohio</option><option value="OK">Oklahoma</option><option value="OR">Oregon</option><option value="PA">Pennsylvania</option><option value="PR">Puerto Rico</option><option value="PW">Palau</option><option value="RI">Rhode Island</option><option value="SC">South Carolina</option><option value="SD">South Dakota</option><option value="TN">Tennessee</option><option value="TX">Texas</option><option value="UT">Utah</option><option value="VA">Virginia</option><option value="VI">Virgin Islands</option><option value="VT">Vermont</option><option value="WA">Washington</option><option value="WI">Wisconsin</option><option value="WV">West Virginia</option><option value="WY">Wyoming</option><option value="AA">Military Americas</option><option value="AE">Military Europe</option><option value="AP">Military Pacific</option><option value="AB">Alberta</option><option value="MB">Manitoba</option><option value="BC">British Columbia</option><option value="NB">New Brunswick</option><option value="NL">Newfoundland and Labrador</option><option value="NS">Nova Scotia</option><option value="NT">Northwest Territories</option><option value="NU">Nunavut</option><option value="ON">Ontario</option><option value="PE">Prince Edward Island</option><option value="QC">Quebec</option><option value="SK">Saskatchewan</option><option value="YT">Yukon Territory</option></select></div></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B4"><div class="customtext section"><div class="form_row clearfix"><div class="form_leftcol"><div class="form_leftcollabel"><label for="SID_Eloqua_Form_C_City">City</label></div><div class="form_leftcolmark"> *</div></div><div class="form_rightcol " id="C_City_rightcol"><div id="C_City_0_wrapper" class="form_rightcol_wrapper"><input maxlength="" onclick="errorCheck('true', 'FormIntent')" class="form_field form_field_text" id="SID_Eloqua_Form_C_City" name="C_City" value="" size="35"></div></div></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B5"><div class="customtext section"><div class="form_row clearfix"><div class="form_leftcol"><div class="form_leftcollabel"><label for="SID_Eloqua_Form_C_Zip_Postal">Postal Code/Zip</label></div><div class="form_leftcolmark"> *</div></div><div class="form_rightcol " id="C_Zip_Postal_rightcol"><div id="C_Zip_Postal_0_wrapper" class="form_rightcol_wrapper"><input maxlength="" onclick="errorCheck('true', 'FormIntent')" class="form_field form_field_text" id="SID_Eloqua_Form_C_Zip_Postal" name="C_Zip_Postal" value="" size="35"></div></div></div></div></div><div container-id="cq-colctrl-lt3" comp-id="B6"><div class="LTRawHTML section"><style type="text/css"> input[type="radio"] { line-height: 22px; margin-bottom: 6px; margin-left: 5px; margin-right: 3px; } .errors { border: 1px solid #EE3134; background: #fce7e7; color: red; } form .form_field_text { margin-bottom: 2px; width: 98%; max-width: 334px; border: #ddd 1px solid; } form div.section { padding-bottom: 2px; } label { margin-bottom: 2px; } form .form_field_select { width: 100%; max-width: 340px; } form .form_leftcollabel, form .form_leftcollabel label, form .form_leftcolmark { display: inline-block; } form .form_leftcolmark { padding-left: 5px; } form .radio, form .checkbox { padding-left: 0px; } select { margin: 0; padding: 0; border-radius 2px; } .submit input { width: auto; max-width: 100%; } .cq-colctrl-bordered-lt0-c0 { width: 47.055555555556%; padding-right: 2.8749999999995%; } .cq-colctrl-bordered-lt0-c1 { width: 47.055555555556%; margin-left: 2.8749999999995%; } div.brightcoveplayer.section { margin-bottom: 0px; } .modal-backdrop { background: none; } .brightcoveplayer .btn-link { text-align: left; padding: 0px; } div.brightcoveplayer button.btn-link:nth-child(2) { white-space: normal; } div.textimage { margin-bottom: 0px; } div.brightcoveplayer a.link-overlay-play { margin-bottom: 8px; } ul.the-icons.clearfix li b button.btn-link { padding: 0 !important; font-weight: bold; } </style><script> // SID_Eloqua_Form Code v3.0 - Mikal Krall $(document).ready(function () { // set referral source $('[name="Referral_Source"]').val(document.referrer) // set opt-in date $('[name="C_Opt_In_Date1"]').val(Date().toLocaleString()); // set opt-in source $('[name="C_Opt_In_Source1"]').val(location.href); // Querystring function - TC/EMail/CEID/IQL var query = window.location.search.substring(1); var parms = query.split('&'); for (var i = 0; i < parms.length; i++) { var pos = parms[i].indexOf('='); if (pos > 0) { var key = parms[i].substring(0, pos); var val = parms[i].substring(pos + 1); if (key == "tc") { $('[name="Target_Community"]').val(val); } if (key == "en") { $('[name="Email_Name"]').val(val); } if (key == "ce") { $('[name="C_Campaign_Element_ID1"]').val(val); } if (key == "cid") { if ($('[name="C_Campaign_Element_ID1"]').val() == "") { $('[name="C_Campaign_Element_ID1"]').val(val); } } if (key == "iq") { $('[name="C_CRM_IQ_1"]').val(val); } } } // trap form submit to perform client-side validation $("#SID_Eloqua_Form").submit(function () { // check to see if the visitor selected a state - default value selected if ($CQ('select[name="C_State_Prov"]').val() == "selected") { $CQ('select[name="C_State_Prov"] option[value="selected"]').remove();// have to remove this item to place a blank in its place $CQ('select[name="C_State_Prov"]').append('<option selected=selected value="">- Select if applicable -</option>'); // return a blank value }; // gather comments from fields if ($CQ('select[name="C_Lead_Comments1"]').length) { // if lead comments are in dropdown var leadComments = $CQ('select[name="C_Lead_Comments1"] option:checked').val() + ' Comment: ' + $CQ('[name="Addtl_Lead_Comments"]').val(); $CQ('select[name="C_Lead_Comments1"] option:checked').val('') // clear out old comments $CQ('select[name="C_Lead_Comments1"] option:checked').val(leadComments) // set new comments } else if ($CQ('input[name="C_Lead_Comments1"]').length) { // if lead comments are in hidden field var leadComments = $CQ('input[name="C_Lead_Comments1"]').val() + ' Comment: ' + $CQ('[name="Addtl_Lead_Comments"]').val() + ' URL: ' + window.location.href; $CQ('input[name="C_Lead_Comments1"]').val('') // clear out old comments $CQ('input[name="C_Lead_Comments1"]').val(leadComments) // set new comments } var industry_TC = ""; industry_TC = $CQ('#SID_Eloqua_Form_C_Industry').val(); if ($CQ('#SID_Eloqua_Form_C_CMA_KL_Target_Community1').val() == 'EN') { industry_TC = "Environment - " + $CQ('#SID_Eloqua_Form_Environment_Industry').val(); } else if 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.nav-tabs>li>a:hover { background: #54545c; border: 0px solid #d8d8d8; } .nav-tabs>li>a:hover, .nav-tabs>li>a:focus { margin-bottom: 0px; padding-bottom: 0px; color: #fff; border-bottom: red 1px solid; padding: 15px 15px 19px 0px; } .tabworkflowcontainer { background-color: #54545c; } .breakout { margin: 0 calc(50% - 50vw) } .plain-tab { display: block; margin: auto; width: 1200px; } .tab .well-cmp .well { padding: 0px; min-height: 0px; margin-bottom: 0px; background-color: #f3f3f300; } .well-container { background-size: cover; position: relative; z-index: 10; } .tab .well-cmp .well-default { padding: 0px; } .tab-content { margin-bottom: 0px; border: 0; border-bottom: 0px solid #d8d8d8; overflow: auto; /* background-color: #54545c; */ color: #fff; height: 440px; margin-top: 0px; overflow: hidden; } .tab-content a:link, .tab-content a:visited { color: #fff; } .tab-content>.tab-pane { padding: 0px; } .accordion-inner { padding: 9px 10px; color: #fff; } .accordion-inner .accordion-content { padding: 9px 10px; color: #000; } .well .textimage-left .text { padding: 9em 0em 1em 2em; width: 90%; color: #fff; } .tab .well-cmp .textimage-left { height: 440px; background: rgb(0,0,0); background: linear-gradient(0deg, rgba(0,0,0,0.8757878151260504) 0%, rgba(0,0,0,0.17270658263305327) 100%); } .textimage-left p { display: -webkit-box; -webkit-line-clamp: 4; -webkit-box-orient: vertical; overflow: hidden; } .tab .cq-colctrl-lt0 .text p { display: -webkit-box; -webkit-line-clamp: 5; -webkit-box-orient: vertical; overflow: hidden; } .tab .cq-colctrl-lt3 .text p { display: -webkit-box; -webkit-line-clamp: 7; -webkit-box-orient: vertical; overflow: hidden; } .tab .cq-colctrl-lt3 .h3, .tab .cq-colctrl-lt3 h3, .tab .cq-colctrl-lt3 .h4, .tab .cq-colctrl-lt3 h4, .tab .cq-colctrl-lt3 .h5, .tab .cq-colctrl-lt3 h5 { display: -webkit-box; -webkit-line-clamp: 2; -webkit-box-orient: vertical; overflow: hidden; } @media (min-width: 1200px) { .well .textimage-left .text { padding: 9em 0em 1em 2em; width: 70%; color: #fff; } .tab .cq-colctrl-lt3, .tab .cq-colctrl-lt0 { width: 75%; padding-top: 30px; padding-left: 30px; } } @media (max-width: 1199px) and (min-width: 980px) { .plain-tab { display: block; margin: auto; width: 100%; } } @media (max-width: 979px) and (min-width: 768px) { .plain-tab { display: block; margin: auto; width: 100%; } .content-techniques { padding: 36% 3% 0%; } } @media (max-width: 767px) { .plain-tab { display: none; } .tabworkflowcontainer { background-color: #fff; } .content-container { padding: 5%; background-color: #ffffffba; height: 370px; } .well-container { background-size: cover; position: relative; z-index: 10; background-repeat: no-repeat; } } @media screen and (max-width: 479px) { .cq-colctrl-lt3 .text, .cq-colctrl-lt0 .text { color: #000; } } @media screen and (max-width: 1366px) { .nav-tabs { border-bottom: 0px solid #d8d8d8; margin-bottom: 0px; width: calc(43% - 9vw) !important; float: left; height: 390px; background-color: #54545c; padding: 50px 34px 0px 25px; overflow: auto; } } @media (min-width:1200px) { .plain-tab { display: block; margin: auto; width: 1200px; } .content-techniques { padding: 12em 0em 1em 2em; width: 55%; } .nav-tabs { border-bottom: 0px solid #d8d8d8; margin-bottom: 0px; width: calc(43% - 9.5vw); float: left; height: 390px; background-color: #54545c; padding: 50px 34px 0px 25px; overflow: auto; } .tab-content { left: 39%; margin-bottom: 0px; border: 0; border-bottom: 0px solid #d8d8d8; overflow: auto; color: #fff; height: 440px; overflow: auto; position: absolute; width: 61%; } } </style><script> $( document ).ready(function() { /* $( "#myTab" ).prepend( "<a name='techniques'></a><h2 class='techniques-header'>Techniques</h2>" ); */ $( ".tabworkflowcontainer" ).addClass( "breakout dark-gray" ); }); </script><div class="ux-tool-label ">Style Sheet for Komodo Tabs</div></div></div></div><div class="LTRawHTML section"><style> .banner_close{ position: absolute; top: 30%; right: 70px; font-weight: 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