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{"title":"In situ Observation of the State and Stability of Hemoglobin Adsorbed onto Glass Surface by Slab Optical Waveguide (SOWG) Spectroscopy","authors":"Masayoshi Matsui, Akiko Nakahara, Akiko Takatsu, Kenji Kato, Naoki Matsuda","volume":11,"journal":"International Journal of Biomedical and Biological Engineering","pagesStart":160,"pagesEnd":164,"ISSN":"1307-6892","URL":"https:\/\/publications.waset.org\/pdf\/15169","abstract":"The state and stability of hemoglobin adsorbed on the\nglass surface was investigated using slab optical waveguide (SOWG)\nspectroscopy. The peak position of the absorption band of hemoglobin\nadsorbed on the glass surface was same as that of the hemoglobin in\nsolution. This result suggests that no significant denaturation occurred\nby adsorption. The adsorption of hemoglobin is relatively strong that\nthe hemoglobin molecules even remained adsorbed after rinsing the\ncell with buffer solution. The peak shift caused by the reduction of\nadsorbed hemoglobin was also observed.","references":"[1] K. Ito, A. Fujishima, \"An application of optical waveguides to\nelectrochemistry,\" in J. Phys. Chem. Vol. 92, 1988, pp. 7043-7045.\n[2] K. Kato, A. Takatsu, N. Matsuda, R. Azumi, M. Matsumoto, \"A\nslab-optical-waveguide absorption spectroscopy of Langmuir-Blodgett\nfilms with a white light excitation source,\" in Chem. Lett., Vol. 24, 1995,\npp. 437-438.\n[3] D. R. Dunphy, S. B. Mendes, S. S. Saavedra, N. R. Armstrong, \"The\nelectroactive integrated optical waveguide: ultrasensitive\nspectroelectrochemistry of submonolayer adsorbates,\" in Anal. Chem.,\nVol. 69, 1997, pp. 3086-3094.\n[4] K. Tsunoda, T. Umemura, H. Ueno, E. Okuno, H. Akaiwa, \"Adsorption of\nmethylene blue onto silylated silica surfaces, studied using visible\nattenuated total reflection spectroscopy with a slab optical waveguide,\" in\nAppl. Spectrosc. Vol. 57, 2003, 1273-1277.\n[5] N. Matsuda, A. Takatsu, K. Kato, \"Absorption spectra of rhodamine 6G\nby slab optical waveguide spectroscopy,\" in Chem. Lett., Vol. 25, 1996,\npp. 105-106.\n[6] K. Kato, A. Takatsu, N. Matsuda, \"UV-visible slab optical waveguide\nspectroscopy of cytochrome c adsorbed on a liquid-solid interface,\" in\nChem. Lett., Vol. 28, 1999, pp. 31-32.\n[7] Z.-M. Qi, N. Matsuda, A. Takatsu, K. Kato, \"A kinetic study of\ncytochrome c adsorption to hydrophilic glass by broad-band,\ntime-resolved optical waveguide spectroscopy,\" in J. Phys. Chem. B. Vol.\n107, 2003, pp. 6873-6875.\n[8] T. Yoshida, H. Asano, J.H. Santos, Z.-M. Qi, A Takatsu, K. Kato, N.\nMatsuda, \"Studies on adsorption behavior of hemoglobin onto\nhydrophobic surface by using slab optical waveguide spectroscopy,\" in\nElectronics and Communications in Japan, vol. J85-C, No. 12, 2003, pp.\n1070-1074.\n[9] Z.-M. Qi, N. Matsuda, T. Yoshida, H. Asano, A. Takatsu, K. Kato,\n\"Optical waveguide spectrometer based on thin-film glass plates,\" in Opt.\nLett. Vol. 27, No. 22, 2002, pp. 2001-2003.\n[10] J.H. Santos, N. Matsuda, Z.-M. Qi, T. Yoshida, A. Takatsu, K. Kato,\n\"Adsorption behavior of cytochrome c, myoglobin and hemoglobin in a\nquartz surface probed using slab optical waveguide (SOWG)\nspectroscopy ,\" in Anal. Sci. Vol. 19, 2003, pp. 199-204.\n[11] N. Matsuda, J.H. Santos, A. Takatsu, K. Kato, \"Spectroelectrochemical\nstudies on surface immobilized cytochrome c on ITO electrode by slab\noptical waveguide spectroscopy,\" in Thin Solid Films Vol. 438-439, 2003,\npp. 403-406.\n[12] K. Fukuda, H. Ohno, \"Electron transfer reaction of cytochrome c at the\nelectrode surface analyzed with non-contact optical waveguide\nspectroscopy,\" in Electroanalysis Vol. 14, 2002, pp. 605-610.\n[13] K. Fujita, H. Ohno, \"Redox reaction of PEO-modified cytochrome c\nadsorbed on the electrode in ion conductive PEO matrix analyzed with\nnon-contact optical waveguide spectroscopy,\" in Polym. Adv. Technol.\nVol. 14, 2003, pp. 486-491.\n[14] L. Bedouet, H. Adenier, S. Pulvin, C. Bedel-Cloutour, D. Thomas,\n\"Recovery of the oxidative activity of caged bovine haemoglobin after\nUV photolysis,\" in Biochem. Biophys. Res. Commun. Vol. 320, 2004, pp.\n939-944.\n[15] X. Liu, L. Shang, Z. Sun, G. Li, \"Direct electrochemistry of hemoglobin\nin dimethyldioctadecyl ammonium bromide film and its electrocatalysis\nto nitric oxide,\" in J. Biochem. Biophys. Methods, Vol. 62, 2005, pp.\n143-151.\n[16] R. Deslauriers, D. J. Moffatt, I. C. P. Smith, \"Oxygen consumption in\nPlasmodium berghei-infected murine red cells: a direct spectrophotomeric\nassay in intact erythrocytes,\" in Biochimica et Biophysica Acta Vol. 886,\n1986, pp. 319-326.\n[17] C. Causserand Y. Kara, P. Aimar, \"Protein fractionation using selective\nadsorption on clay surface before filtration,\" in J. Membr. Sci. Vol. 186,\n2001, pp. 165-181.\n[18] A.-E. F. Nassar, J. F. Rusling, N. Nakashima, \"Electron transfer between\nelectrodes and heme proteins in protein-DNA films,\" in J. Am. Chem. Soc.\nVol. 118, 1996, pp. 3043-3044.\n[19] Y. Liu, H. Liu, N. Hu \"Core-shell nanocluster films of hemoglobin and\nclay nanoparticle: direct electrochemistry and electrocatalysis,\" in\nBiophys. Chemist. Vol. 117,, 2005, pp. 27-37.\n[20] Z. Dai, S. Liu, H. Ju, H. Chen, \"Direct electron transfer and enzymatic\nactivity of hemoglobin in a hexagonal mesoporous silica matrix,\" in\nBiosens. Bioelectron. Vol. 19, 2004, pp. 861-867.\n[21] H.-Y. Gu, A.-M. Yu, H.-Y. Chen, \"Direct electron transfer and\ncharacterization of hemoglobin immobilized on a Au\ncolloid-cysteamine-modified gold electrode,\" in J. Electroanal. Chem.\nVol. 516, 2001, pp. 119-126.\n[22] A. Kondo, S. Oku, F. Murakami, K. Higashitani \"Conformational changes\nin protein molecules upon adsorption on ultrafine particles,\" in Colloids\nSurf. B: Biointerf. Vol. 1, 1993, pp. 197-201.\n[23] A. Kondo, J. Mihara \"Comparison of adsorption and conformation of\nhemoglobin and myoglobin on various inorganic ultrafine particles,\" in J.\nColloid Interface Sci. Vol. 177, 1996, pp. 214-221.\n[24] B.A. McCool, R. Cashon, G. Karles, W.J. DeSisto, \"Silica encapsulated\nhemoglobin and myoglobin powders prepared by an aqueous\nfast-freezing technique ,\" in J. Non-Cryst. solids Vol. 333, 2004, pp.\n143-149.\n[25] J. Chen, J.D. Andrade, R.A. VanWagenen, \"Oxy-and deoxyhaemoglobin\nadsorption onto glass and polymer surfaces,\" in Biomaterials Vol. 6,\n1985, pp. 231-236.\n[26] R. F H. FREEMAN, \"Haemoglobin in the digenetic trematode proctoeces\nsubtenuis (linton),\" in Comp. Biochem. Physiol. Vol. 10, 1963, pp.\n253-256.\n[27] H-Y. Chen, Y-T. Long, \"Study of biomolecules by combining\nelectrochemistry with UV\/Vis, IR and surface enhanced Raman scattering\nspectroscopy by a novel flow miccrocell,\" in Anal. Chem. Acta Vol. 382,\n1999, pp. 171-177.","publisher":"World Academy of Science, Engineering and Technology","index":"Open Science Index 11, 2007"}